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Protein

Sodium-dependent dopamine transporter

Gene

Slc6a3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Amine transporter. Terminates the action of dopamine by its high affinity sodium-dependent reuptake into presynaptic terminals.Curated2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi75Sodium 1; via carbonyl oxygenBy similarity1
Metal bindingi77Sodium 2; via carbonyl oxygenBy similarity1
Metal bindingi78Sodium 1; via carbonyl oxygenBy similarity1
Metal bindingi82Sodium 2By similarity1
Sitei105Contributes to high-affinity binding to cocaine1 Publication1
Metal bindingi320Sodium 2By similarity1
Metal bindingi352Sodium 2By similarity1
Metal bindingi417Sodium 1; via carbonyl oxygenBy similarity1
Metal bindingi420Sodium 1By similarity1
Metal bindingi421Sodium 1By similarity1

GO - Molecular functioni

  • dopamine:sodium symporter activity Source: GO_Central
  • dopamine binding Source: MGI
  • dopamine transmembrane transporter activity Source: MGI
  • drug binding Source: Ensembl
  • metal ion binding Source: UniProtKB-KW
  • monoamine transmembrane transporter activity Source: MGI
  • protein N-terminus binding Source: UniProtKB

GO - Biological processi

  • adenohypophysis development Source: MGI
  • aging Source: Ensembl
  • dopamine biosynthetic process Source: MGI
  • dopamine catabolic process Source: MGI
  • dopamine transport Source: MGI
  • dopamine uptake Source: MGI
  • dopamine uptake involved in synaptic transmission Source: GO_Central
  • lactation Source: MGI
  • locomotory behavior Source: MGI
  • monoamine transport Source: MGI
  • positive regulation of multicellular organism growth Source: MGI
  • prepulse inhibition Source: MGI
  • regulation of dopamine metabolic process Source: MGI
  • response to cAMP Source: Ensembl
  • response to cocaine Source: MGI
  • response to drug Source: Ensembl
  • response to ethanol Source: Ensembl
  • response to iron ion Source: Ensembl
  • response to nicotine Source: Ensembl
  • sensory perception of smell Source: MGI
Complete GO annotation...

Keywords - Biological processi

Neurotransmitter transport, Symport, Transport

Keywords - Ligandi

Metal-binding, Sodium

Enzyme and pathway databases

ReactomeiR-MMU-379401. Dopamine clearance from the synaptic cleft.
R-MMU-442660. Na+/Cl- dependent neurotransmitter transporters.

Names & Taxonomyi

Protein namesi
Recommended name:
Sodium-dependent dopamine transporter
Short name:
DA transporter
Short name:
DAT
Alternative name(s):
Solute carrier family 6 member 3
Gene namesi
Name:Slc6a3
Synonyms:Dat, Dat1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 13

Organism-specific databases

MGIiMGI:94862. Slc6a3.

Subcellular locationi

  • Cell membrane 2 Publications; Multi-pass membrane protein By similarity

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 68CytoplasmicSequence analysisAdd BLAST68
Transmembranei69 – 89Helical; Name=1Sequence analysisAdd BLAST21
Transmembranei96 – 116Helical; Name=2Sequence analysisAdd BLAST21
Transmembranei140 – 160Helical; Name=3Sequence analysisAdd BLAST21
Topological domaini161 – 236ExtracellularSequence analysisAdd BLAST76
Transmembranei237 – 255Helical; Name=4Sequence analysisAdd BLAST19
Transmembranei264 – 281Helical; Name=5Sequence analysisAdd BLAST18
Transmembranei317 – 334Helical; Name=6Sequence analysisAdd BLAST18
Transmembranei346 – 367Helical; Name=7Sequence analysisAdd BLAST22
Transmembranei400 – 419Helical; Name=8Sequence analysisAdd BLAST20
Transmembranei446 – 464Helical; Name=9Sequence analysisAdd BLAST19
Transmembranei480 – 500Helical; Name=10Sequence analysisAdd BLAST21
Transmembranei521 – 540Helical; Name=11Sequence analysisAdd BLAST20
Transmembranei559 – 577Helical; Name=12Sequence analysisAdd BLAST19
Topological domaini578 – 619CytoplasmicSequence analysisAdd BLAST42

GO - Cellular componenti

  • axon Source: MGI
  • cell surface Source: UniProtKB
  • flotillin complex Source: MGI
  • integral component of plasma membrane Source: GO_Central
  • membrane raft Source: MGI
  • neuronal cell body Source: MGI
  • neuron projection Source: MGI
  • plasma membrane Source: MGI
  • presynapse Source: GOC
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi105F → A, I, L, N, Q, S or T: Very low transporter activity. 1 Publication1
Mutagenesisi105F → C: Reduced sensitivity to cocaine. 1 Publication1
Mutagenesisi105F → M: Functional transporter with 4-fold decrease in cocaine sensitivity. 1 Publication1
Mutagenesisi105F → W or Y: Retention of transporter activity and high sensitivity to cocaine. 1 Publication1

Chemistry databases

ChEMBLiCHEMBL2799.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002147531 – 619Sodium-dependent dopamine transporterAdd BLAST619

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi180 ↔ 189By similarity
Glycosylationi181N-linked (GlcNAc...)Sequence analysis1
Glycosylationi188N-linked (GlcNAc...)Sequence analysis1
Glycosylationi196N-linked (GlcNAc...)Sequence analysis1
Glycosylationi204N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi305InterchainBy similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiQ61327.
PaxDbiQ61327.
PRIDEiQ61327.

PTM databases

iPTMnetiQ61327.
PhosphoSitePlusiQ61327.

Expressioni

Tissue specificityi

Found in the substantia nigra and ventral tegmental dopamine neurons, in fibers of the medial forebrain bundle ascending into the striatum, and within dense fiber networks and varicosities in the dorsal and ventral striatum. Lower expression in the cortex. Absent from the corpus callosum.1 Publication

Gene expression databases

BgeeiENSMUSG00000021609.
GenevisibleiQ61327. MM.

Interactioni

Subunit structurei

Homooligomer; disulfide-linked (By similarity). Interacts with PRKCABP and TGFB1I1. Interacts (via N-terminus) with SYNGR3 (via N-terminus). Interacts with SLC18A2. Interacts with TOR1A (ATP-bound); TOR1A regulates SLC6A3 subcellular location.By similarity2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
SNCAP378405EBI-7839708,EBI-985879From a different organism.
SncaO550425EBI-7839708,EBI-2310271

GO - Molecular functioni

  • protein N-terminus binding Source: UniProtKB

Protein-protein interaction databases

BioGridi199053. 2 interactors.
DIPiDIP-41828N.
IntActiQ61327. 2 interactors.
MINTiMINT-576974.
STRINGi10090.ENSMUSP00000022100.

Chemistry databases

BindingDBiQ61327.

Structurei

3D structure databases

ProteinModelPortaliQ61327.
SMRiQ61327.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni560 – 589Interaction with TGFB1I1By similarityAdd BLAST30

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3659. Eukaryota.
COG0733. LUCA.
GeneTreeiENSGT00760000118857.
HOGENOMiHOG000116406.
HOVERGENiHBG071421.
InParanoidiQ61327.
KOiK05036.
OMAiYAAYKFC.
OrthoDBiEOG091G08PX.
PhylomeDBiQ61327.
TreeFamiTF343812.

Family and domain databases

InterProiIPR000175. Na/ntran_symport.
IPR002436. Na/ntran_symport_dopamine.
[Graphical view]
PANTHERiPTHR11616. PTHR11616. 1 hit.
PfamiPF00209. SNF. 1 hit.
[Graphical view]
PRINTSiPR01202. DOPTRANSPORT.
PR00176. NANEUSMPORT.
PROSITEiPS00610. NA_NEUROTRAN_SYMP_1. 1 hit.
PS00754. NA_NEUROTRAN_SYMP_2. 1 hit.
PS50267. NA_NEUROTRAN_SYMP_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q61327-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKSKCSVGP MSSVVAPAKE PNAVGPREVE LILVKEQNGV QLTNSTLINP
60 70 80 90 100
PQTPVEVQER ETWSKKIDFL LSVIGFAVDL ANVWRFPYLC YKNGGGAFLV
110 120 130 140 150
PYLLFMVIAG MPLFYMELAL GQFNREGAAG VWKICPVLKG VGFTVILISF
160 170 180 190 200
YVGFFYNVII AWALHYFFSS FTMDLPWIHC NNTWNSPNCS DAHSSNSSDG
210 220 230 240 250
LGLNDTFGTT PAAEYFERGV LHLHQSRGID DLGPPRWQLT ACLVLVIVLL
260 270 280 290 300
YFSLWKGVKT SGKVVWITAT MPYVVLTALL LRGVTLPGAM DGIRAYLSVD
310 320 330 340 350
FYRLCEASVW IDAATQVCFS LGVGFGVLIA FSSYNKFTNN CYRDAIITTS
360 370 380 390 400
INSLTSFSSG FVVFSFLGYM AQKHNVPIRD VATDGPGLIF IIYPEAIATL
410 420 430 440 450
PLSSAWAAVF FLMLLTLGID SAMGGMESVI TGLVDEFQLL HRHRELFTLG
460 470 480 490 500
IVLATFLLSL FCVTNGGIYV FTLLDHFAAG TSILFGVLIE AIGVAWFYGV
510 520 530 540 550
QQFSDDIKQM TGQRPNLYWR LCWKLVSPCF LLYVVVVSIV TFRPPHYGAY
560 570 580 590 600
IFPDWANALG WIIATSSMAM VPIYATYKFC SLPGSFREKL AYAITPEKDR
610
QLVDRGEVRQ FTLRHWLLV
Length:619
Mass (Da):68,805
Last modified:May 27, 2002 - v2
Checksum:iF436137BCE461E9B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF109072 mRNA. Translation: AAD19643.1.
BC054119 mRNA. Translation: AAH54119.1.
AH003328 Genomic DNA. Translation: AAA86462.2.
U16265 mRNA. Translation: AAC52283.1.
CCDSiCCDS26632.1.
RefSeqiNP_034150.1. NM_010020.3.
UniGeneiMm.41993.

Genome annotation databases

EnsembliENSMUST00000022100; ENSMUSP00000022100; ENSMUSG00000021609.
GeneIDi13162.
KEGGimmu:13162.
UCSCiuc007rdn.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF109072 mRNA. Translation: AAD19643.1.
BC054119 mRNA. Translation: AAH54119.1.
AH003328 Genomic DNA. Translation: AAA86462.2.
U16265 mRNA. Translation: AAC52283.1.
CCDSiCCDS26632.1.
RefSeqiNP_034150.1. NM_010020.3.
UniGeneiMm.41993.

3D structure databases

ProteinModelPortaliQ61327.
SMRiQ61327.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi199053. 2 interactors.
DIPiDIP-41828N.
IntActiQ61327. 2 interactors.
MINTiMINT-576974.
STRINGi10090.ENSMUSP00000022100.

Chemistry databases

BindingDBiQ61327.
ChEMBLiCHEMBL2799.

PTM databases

iPTMnetiQ61327.
PhosphoSitePlusiQ61327.

Proteomic databases

MaxQBiQ61327.
PaxDbiQ61327.
PRIDEiQ61327.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000022100; ENSMUSP00000022100; ENSMUSG00000021609.
GeneIDi13162.
KEGGimmu:13162.
UCSCiuc007rdn.1. mouse.

Organism-specific databases

CTDi6531.
MGIiMGI:94862. Slc6a3.

Phylogenomic databases

eggNOGiKOG3659. Eukaryota.
COG0733. LUCA.
GeneTreeiENSGT00760000118857.
HOGENOMiHOG000116406.
HOVERGENiHBG071421.
InParanoidiQ61327.
KOiK05036.
OMAiYAAYKFC.
OrthoDBiEOG091G08PX.
PhylomeDBiQ61327.
TreeFamiTF343812.

Enzyme and pathway databases

ReactomeiR-MMU-379401. Dopamine clearance from the synaptic cleft.
R-MMU-442660. Na+/Cl- dependent neurotransmitter transporters.

Miscellaneous databases

PROiQ61327.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000021609.
GenevisibleiQ61327. MM.

Family and domain databases

InterProiIPR000175. Na/ntran_symport.
IPR002436. Na/ntran_symport_dopamine.
[Graphical view]
PANTHERiPTHR11616. PTHR11616. 1 hit.
PfamiPF00209. SNF. 1 hit.
[Graphical view]
PRINTSiPR01202. DOPTRANSPORT.
PR00176. NANEUSMPORT.
PROSITEiPS00610. NA_NEUROTRAN_SYMP_1. 1 hit.
PS00754. NA_NEUROTRAN_SYMP_2. 1 hit.
PS50267. NA_NEUROTRAN_SYMP_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSC6A3_MOUSE
AccessioniPrimary (citable) accession number: Q61327
Secondary accession number(s): Q60719, Q9R1I2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: May 27, 2002
Last modified: November 2, 2016
This is version 150 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

This protein is the target of psychomotor stimulants such as amphetamines and cocaine.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.