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Protein

Sodium-dependent dopamine transporter

Gene

Slc6a3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Amine transporter. Terminates the action of dopamine by its high affinity sodium-dependent reuptake into presynaptic terminals.Curated2 Publications

Miscellaneous

This protein is the target of psychomotor stimulants such as amphetamines and cocaine.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi75Sodium 1; via carbonyl oxygenBy similarity1
Metal bindingi77Sodium 2; via carbonyl oxygenBy similarity1
Metal bindingi78Sodium 1; via carbonyl oxygenBy similarity1
Metal bindingi82Sodium 2By similarity1
Sitei105Contributes to high-affinity binding to cocaine1 Publication1
Metal bindingi320Sodium 2By similarity1
Metal bindingi352Sodium 2By similarity1
Metal bindingi417Sodium 1; via carbonyl oxygenBy similarity1
Metal bindingi420Sodium 1By similarity1
Metal bindingi421Sodium 1By similarity1

GO - Molecular functioni

  • dopamine:sodium symporter activity Source: GO_Central
  • dopamine binding Source: MGI
  • dopamine transmembrane transporter activity Source: MGI
  • metal ion binding Source: UniProtKB-KW
  • monoamine transmembrane transporter activity Source: MGI
  • neurotransmitter:sodium symporter activity Source: InterPro
  • protein-containing complex binding Source: Ensembl
  • protein N-terminus binding Source: UniProtKB

GO - Biological processi

  • adenohypophysis development Source: MGI
  • aging Source: Ensembl
  • dopamine biosynthetic process Source: MGI
  • dopamine catabolic process Source: MGI
  • dopamine transport Source: MGI
  • dopamine uptake Source: MGI
  • dopamine uptake involved in synaptic transmission Source: GO_Central
  • lactation Source: MGI
  • locomotory behavior Source: MGI
  • monoamine transport Source: MGI
  • positive regulation of multicellular organism growth Source: MGI
  • prepulse inhibition Source: MGI
  • regulation of dopamine metabolic process Source: MGI
  • response to cAMP Source: Ensembl
  • response to cocaine Source: MGI
  • response to ethanol Source: Ensembl
  • response to iron ion Source: Ensembl
  • response to nicotine Source: Ensembl
  • sensory perception of smell Source: MGI

Keywordsi

Biological processNeurotransmitter transport, Symport, Transport
LigandMetal-binding, Sodium

Enzyme and pathway databases

ReactomeiR-MMU-379401 Dopamine clearance from the synaptic cleft
R-MMU-442660 Na+/Cl- dependent neurotransmitter transporters

Names & Taxonomyi

Protein namesi
Recommended name:
Sodium-dependent dopamine transporter
Short name:
DA transporter
Short name:
DAT
Alternative name(s):
Solute carrier family 6 member 3
Gene namesi
Name:Slc6a3
Synonyms:Dat, Dat1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 13

Organism-specific databases

MGIiMGI:94862 Slc6a3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 68CytoplasmicSequence analysisAdd BLAST68
Transmembranei69 – 89Helical; Name=1Sequence analysisAdd BLAST21
Transmembranei96 – 116Helical; Name=2Sequence analysisAdd BLAST21
Transmembranei140 – 160Helical; Name=3Sequence analysisAdd BLAST21
Topological domaini161 – 236ExtracellularSequence analysisAdd BLAST76
Transmembranei237 – 255Helical; Name=4Sequence analysisAdd BLAST19
Transmembranei264 – 281Helical; Name=5Sequence analysisAdd BLAST18
Transmembranei317 – 334Helical; Name=6Sequence analysisAdd BLAST18
Transmembranei346 – 367Helical; Name=7Sequence analysisAdd BLAST22
Transmembranei400 – 419Helical; Name=8Sequence analysisAdd BLAST20
Transmembranei446 – 464Helical; Name=9Sequence analysisAdd BLAST19
Transmembranei480 – 500Helical; Name=10Sequence analysisAdd BLAST21
Transmembranei521 – 540Helical; Name=11Sequence analysisAdd BLAST20
Transmembranei559 – 577Helical; Name=12Sequence analysisAdd BLAST19
Topological domaini578 – 619CytoplasmicSequence analysisAdd BLAST42

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi105F → A, I, L, N, Q, S or T: Very low transporter activity. 1 Publication1
Mutagenesisi105F → C: Reduced sensitivity to cocaine. 1 Publication1
Mutagenesisi105F → M: Functional transporter with 4-fold decrease in cocaine sensitivity. 1 Publication1
Mutagenesisi105F → W or Y: Retention of transporter activity and high sensitivity to cocaine. 1 Publication1

Chemistry databases

ChEMBLiCHEMBL2799

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002147531 – 619Sodium-dependent dopamine transporterAdd BLAST619

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi180 ↔ 189By similarity
Glycosylationi181N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi188N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi196N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi204N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi305InterchainBy similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiQ61327
PaxDbiQ61327
PRIDEiQ61327

PTM databases

iPTMnetiQ61327
PhosphoSitePlusiQ61327
SwissPalmiQ61327

Expressioni

Tissue specificityi

Found in the substantia nigra and ventral tegmental dopamine neurons, in fibers of the medial forebrain bundle ascending into the striatum, and within dense fiber networks and varicosities in the dorsal and ventral striatum. Lower expression in the cortex. Absent from the corpus callosum.1 Publication

Gene expression databases

BgeeiENSMUSG00000021609
GenevisibleiQ61327 MM

Interactioni

Subunit structurei

Homooligomer; disulfide-linked (By similarity). Interacts with PRKCABP and TGFB1I1. Interacts (via N-terminus) with SYNGR3 (via N-terminus). Interacts with SLC18A2. Interacts with TOR1A (ATP-bound); TOR1A regulates SLC6A3 subcellular location.By similarity2 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

  • protein N-terminus binding Source: UniProtKB

Protein-protein interaction databases

BioGridi199053, 2 interactors
CORUMiQ61327
DIPiDIP-41828N
IntActiQ61327, 5 interactors
MINTiQ61327
STRINGi10090.ENSMUSP00000022100

Chemistry databases

BindingDBiQ61327

Structurei

3D structure databases

ProteinModelPortaliQ61327
SMRiQ61327
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni560 – 589Interaction with TGFB1I1By similarityAdd BLAST30

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3659 Eukaryota
COG0733 LUCA
GeneTreeiENSGT00760000118857
HOGENOMiHOG000116406
HOVERGENiHBG071421
InParanoidiQ61327
KOiK05036
OMAiGMATFIF
OrthoDBiEOG091G08PX
PhylomeDBiQ61327
TreeFamiTF343812

Family and domain databases

InterProiView protein in InterPro
IPR000175 Na/ntran_symport
IPR002436 Na/ntran_symport_dopamine
IPR037272 SNS_sf
PANTHERiPTHR11616 PTHR11616, 1 hit
PfamiView protein in Pfam
PF00209 SNF, 1 hit
PRINTSiPR01202 DOPTRANSPORT
PR00176 NANEUSMPORT
SUPFAMiSSF161070 SSF161070, 1 hit
PROSITEiView protein in PROSITE
PS00610 NA_NEUROTRAN_SYMP_1, 1 hit
PS00754 NA_NEUROTRAN_SYMP_2, 1 hit
PS50267 NA_NEUROTRAN_SYMP_3, 1 hit

Sequencei

Sequence statusi: Complete.

Q61327-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKSKCSVGP MSSVVAPAKE PNAVGPREVE LILVKEQNGV QLTNSTLINP
60 70 80 90 100
PQTPVEVQER ETWSKKIDFL LSVIGFAVDL ANVWRFPYLC YKNGGGAFLV
110 120 130 140 150
PYLLFMVIAG MPLFYMELAL GQFNREGAAG VWKICPVLKG VGFTVILISF
160 170 180 190 200
YVGFFYNVII AWALHYFFSS FTMDLPWIHC NNTWNSPNCS DAHSSNSSDG
210 220 230 240 250
LGLNDTFGTT PAAEYFERGV LHLHQSRGID DLGPPRWQLT ACLVLVIVLL
260 270 280 290 300
YFSLWKGVKT SGKVVWITAT MPYVVLTALL LRGVTLPGAM DGIRAYLSVD
310 320 330 340 350
FYRLCEASVW IDAATQVCFS LGVGFGVLIA FSSYNKFTNN CYRDAIITTS
360 370 380 390 400
INSLTSFSSG FVVFSFLGYM AQKHNVPIRD VATDGPGLIF IIYPEAIATL
410 420 430 440 450
PLSSAWAAVF FLMLLTLGID SAMGGMESVI TGLVDEFQLL HRHRELFTLG
460 470 480 490 500
IVLATFLLSL FCVTNGGIYV FTLLDHFAAG TSILFGVLIE AIGVAWFYGV
510 520 530 540 550
QQFSDDIKQM TGQRPNLYWR LCWKLVSPCF LLYVVVVSIV TFRPPHYGAY
560 570 580 590 600
IFPDWANALG WIIATSSMAM VPIYATYKFC SLPGSFREKL AYAITPEKDR
610
QLVDRGEVRQ FTLRHWLLV
Length:619
Mass (Da):68,805
Last modified:May 27, 2002 - v2
Checksum:iF436137BCE461E9B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF109072 mRNA Translation: AAD19643.1
BC054119 mRNA Translation: AAH54119.1
AH003328 Genomic DNA Translation: AAA86462.2
U16265 mRNA Translation: AAC52283.1
CCDSiCCDS26632.1
RefSeqiNP_034150.1, NM_010020.3
UniGeneiMm.41993

Genome annotation databases

EnsembliENSMUST00000022100; ENSMUSP00000022100; ENSMUSG00000021609
GeneIDi13162
KEGGimmu:13162
UCSCiuc007rdn.1 mouse

Similar proteinsi

Entry informationi

Entry nameiSC6A3_MOUSE
AccessioniPrimary (citable) accession number: Q61327
Secondary accession number(s): Q60719, Q9R1I2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: May 27, 2002
Last modified: May 23, 2018
This is version 160 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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