Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q61327 (SC6A3_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 125. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Sodium-dependent dopamine transporter

Short name=DA transporter
Short name=DAT
Alternative name(s):
Solute carrier family 6 member 3
Gene names
Name:Slc6a3
Synonyms:Dat, Dat1
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length619 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Amine transporter. Terminates the action of dopamine by its high affinity sodium-dependent reuptake into presynaptic terminals By similarity.

Subunit structure

Homooligomer; disulfide-linked. Interacts with PRKCABP By similarity. Interacts with TGFB1I1. Interacts (via N-terminus) with SYNGR3 (via N-terminus). Interacts with SLC18A2. Ref.4 Ref.5

Subcellular location

Membrane; Multi-pass membrane protein By similarity.

Tissue specificity

Found in the substantia nigra and ventral tegmental dopamine neurons, in fibers of the medial forebrain bundle ascending into the striatum, and within dense fiber networks and varicosities in the dorsal and ventral striatum. Lower expression in the cortex. Absent from the corpus callosum. Ref.5

Miscellaneous

This protein is the target of psychomotor stimulants such as amphetamines and cocaine.

Sequence similarities

Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family. SLC6A3 subfamily. [View classification]

Ontologies

Keywords
   Biological processNeurotransmitter transport
Symport
Transport
   Cellular componentMembrane
   DomainTransmembrane
Transmembrane helix
   PTMDisulfide bond
Glycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processadenohypophysis development

Inferred from mutant phenotype PubMed 9247269. Source: MGI

aging

Inferred from electronic annotation. Source: Ensembl

dopamine biosynthetic process

Inferred from mutant phenotype PubMed 9520487. Source: MGI

dopamine catabolic process

Inferred from mutant phenotype PubMed 9520487. Source: MGI

dopamine transport

Inferred from mutant phenotype PubMed 9520487. Source: MGI

lactation

Inferred from mutant phenotype PubMed 9247269. Source: MGI

locomotory behavior

Inferred from mutant phenotype PubMed 11150348. Source: MGI

monoamine transport

Inferred from sequence orthology PubMed 16024787. Source: MGI

positive regulation of multicellular organism growth

Inferred from mutant phenotype PubMed 9247269. Source: MGI

prepulse inhibition

Inferred from mutant phenotype PubMed 11150348. Source: MGI

regulation of dopamine metabolic process

Inferred from mutant phenotype PubMed 9247269PubMed 9520487. Source: MGI

response to cAMP

Inferred from electronic annotation. Source: Ensembl

response to cocaine

Inferred from direct assay PubMed 18671743. Source: MGI

response to drug

Inferred from electronic annotation. Source: Ensembl

response to ethanol

Inferred from electronic annotation. Source: Ensembl

response to iron ion

Inferred from electronic annotation. Source: Ensembl

response to nicotine

Inferred from electronic annotation. Source: Ensembl

sensory perception of smell

Inferred from mutant phenotype PubMed 16765459. Source: MGI

   Cellular_componentaxon

Inferred from direct assay PubMed 17761882. Source: MGI

integral component of plasma membrane

Inferred from electronic annotation. Source: InterPro

neuronal cell body

Inferred from electronic annotation. Source: Ensembl

   Molecular_functiondopamine binding

Inferred from direct assay PubMed 18671743. Source: MGI

dopamine transmembrane transporter activity

Inferred from mutant phenotype PubMed 9520487. Source: MGI

dopamine:sodium symporter activity

Inferred from electronic annotation. Source: Ensembl

drug binding

Inferred from electronic annotation. Source: Ensembl

monoamine transmembrane transporter activity

Inferred from sequence orthology PubMed 16024787. Source: MGI

protein N-terminus binding

Inferred from physical interaction Ref.5. Source: UniProtKB

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

SNCAP378405EBI-7839708,EBI-985879From a different organism.
SncaO550425EBI-7839708,EBI-2310271

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 619619Sodium-dependent dopamine transporter
PRO_0000214753

Regions

Topological domain1 – 6868Cytoplasmic Potential
Transmembrane69 – 8921Helical; Name=1; Potential
Transmembrane96 – 11621Helical; Name=2; Potential
Transmembrane140 – 16021Helical; Name=3; Potential
Topological domain161 – 23676Extracellular Potential
Transmembrane237 – 25519Helical; Name=4; Potential
Transmembrane264 – 28118Helical; Name=5; Potential
Transmembrane317 – 33418Helical; Name=6; Potential
Transmembrane346 – 36722Helical; Name=7; Potential
Transmembrane400 – 41920Helical; Name=8; Potential
Transmembrane446 – 46419Helical; Name=9; Potential
Transmembrane480 – 50021Helical; Name=10; Potential
Transmembrane521 – 54020Helical; Name=11; Potential
Transmembrane559 – 57719Helical; Name=12; Potential
Topological domain578 – 61942Cytoplasmic Potential
Region560 – 58930Interaction with TGFB1I1 By similarity

Amino acid modifications

Glycosylation1811N-linked (GlcNAc...) Potential
Glycosylation1881N-linked (GlcNAc...) Potential
Glycosylation1961N-linked (GlcNAc...) Potential
Glycosylation2041N-linked (GlcNAc...) Potential
Disulfide bond305Interchain By similarity

Sequences

Sequence LengthMass (Da)Tools
Q61327 [UniParc].

Last modified May 27, 2002. Version 2.
Checksum: F436137BCE461E9B

FASTA61968,805
        10         20         30         40         50         60 
MSKSKCSVGP MSSVVAPAKE PNAVGPREVE LILVKEQNGV QLTNSTLINP PQTPVEVQER 

        70         80         90        100        110        120 
ETWSKKIDFL LSVIGFAVDL ANVWRFPYLC YKNGGGAFLV PYLLFMVIAG MPLFYMELAL 

       130        140        150        160        170        180 
GQFNREGAAG VWKICPVLKG VGFTVILISF YVGFFYNVII AWALHYFFSS FTMDLPWIHC 

       190        200        210        220        230        240 
NNTWNSPNCS DAHSSNSSDG LGLNDTFGTT PAAEYFERGV LHLHQSRGID DLGPPRWQLT 

       250        260        270        280        290        300 
ACLVLVIVLL YFSLWKGVKT SGKVVWITAT MPYVVLTALL LRGVTLPGAM DGIRAYLSVD 

       310        320        330        340        350        360 
FYRLCEASVW IDAATQVCFS LGVGFGVLIA FSSYNKFTNN CYRDAIITTS INSLTSFSSG 

       370        380        390        400        410        420 
FVVFSFLGYM AQKHNVPIRD VATDGPGLIF IIYPEAIATL PLSSAWAAVF FLMLLTLGID 

       430        440        450        460        470        480 
SAMGGMESVI TGLVDEFQLL HRHRELFTLG IVLATFLLSL FCVTNGGIYV FTLLDHFAAG 

       490        500        510        520        530        540 
TSILFGVLIE AIGVAWFYGV QQFSDDIKQM TGQRPNLYWR LCWKLVSPCF LLYVVVVSIV 

       550        560        570        580        590        600 
TFRPPHYGAY IFPDWANALG WIIATSSMAM VPIYATYKFC SLPGSFREKL AYAITPEKDR 

       610 
QLVDRGEVRQ FTLRHWLLV 

« Hide

References

« Hide 'large scale' references
[1]"Molecular cloning of the mouse dopamine transporter and pharmacological comparison with the human homologue."
Wu X., Gu H.H.
Gene 233:163-170(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: C57BL/6J.
Tissue: Brain.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Brain.
[3]"Human and mouse dopamine transporter genes: conservation of 5'-flanking sequence elements and gene structures."
Donovan D.M., Vandenbergh D.J., Perry M.P., Bird G.S., Ingersoll R., Nanthakumar E., Uhl G.R.
Brain Res. Mol. Brain Res. 30:327-335(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-343.
Strain: BALB/c.
Tissue: Brain.
[4]"The multiple LIM domain-containing adaptor protein Hic-5 synaptically colocalizes and interacts with the dopamine transporter."
Carneiro A.M.D., Ingram S.L., Beaulieu J.-M., Sweeney A., Amara S.G., Thomas S.M., Caron M.G., Torres G.E.
J. Neurosci. 22:7045-7054(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH TGFB1I1.
[5]"Physical and functional interaction between the dopamine transporter and the synaptic vesicle protein synaptogyrin-3."
Egana L.A., Cuevas R.A., Baust T.B., Parra L.A., Leak R.K., Hochendoner S., Pena K., Quiroz M., Hong W.C., Dorostkar M.M., Janz R., Sitte H.H., Torres G.E.
J. Neurosci. 29:4592-4604(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH SYNGR3 AND SLC18A2, TISSUE SPECIFICITY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF109072 mRNA. Translation: AAD19643.1.
BC054119 mRNA. Translation: AAH54119.1.
AH003328 Genomic DNA. Translation: AAA86462.2.
U16265 mRNA. Translation: AAC52283.1.
RefSeqNP_034150.1. NM_010020.3.
XP_006517145.1. XM_006517082.1.
UniGeneMm.41993.

3D structure databases

ProteinModelPortalQ61327.
SMRQ61327. Positions 59-599.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid199053. 2 interactions.
IntActQ61327. 2 interactions.
MINTMINT-576974.

Chemistry

BindingDBQ61327.
ChEMBLCHEMBL2799.

PTM databases

PhosphoSiteQ61327.

Proteomic databases

PaxDbQ61327.
PRIDEQ61327.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000022100; ENSMUSP00000022100; ENSMUSG00000021609.
GeneID13162.
KEGGmmu:13162.
UCSCuc007rdn.1. mouse.

Organism-specific databases

CTD6531.
MGIMGI:94862. Slc6a3.

Phylogenomic databases

eggNOGCOG0733.
GeneTreeENSGT00740000115174.
HOGENOMHOG000116406.
HOVERGENHBG071421.
InParanoidQ61327.
KOK05036.
OMASPNCSDA.
OrthoDBEOG793B71.
PhylomeDBQ61327.
TreeFamTF343812.

Gene expression databases

BgeeQ61327.
GenevestigatorQ61327.

Family and domain databases

InterProIPR000175. Na/ntran_symport.
IPR002436. Na/ntran_symport_dopamine.
[Graphical view]
PANTHERPTHR11616. PTHR11616. 1 hit.
PfamPF00209. SNF. 1 hit.
[Graphical view]
PRINTSPR01202. DOPTRANSPORT.
PR00176. NANEUSMPORT.
PROSITEPS00610. NA_NEUROTRAN_SYMP_1. 1 hit.
PS00754. NA_NEUROTRAN_SYMP_2. 1 hit.
PS50267. NA_NEUROTRAN_SYMP_3. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio283234.
PROQ61327.
SOURCESearch...

Entry information

Entry nameSC6A3_MOUSE
AccessionPrimary (citable) accession number: Q61327
Secondary accession number(s): Q60719, Q9R1I2
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: May 27, 2002
Last modified: April 16, 2014
This is version 125 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot