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Protein

Sodium-dependent dopamine transporter

Gene

Slc6a3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Amine transporter. Terminates the action of dopamine by its high affinity sodium-dependent reuptake into presynaptic terminals (By similarity).By similarity

GO - Molecular functioni

  1. dopamine:sodium symporter activity Source: Ensembl
  2. dopamine binding Source: MGI
  3. dopamine transmembrane transporter activity Source: MGI
  4. drug binding Source: Ensembl
  5. monoamine transmembrane transporter activity Source: MGI
  6. protein N-terminus binding Source: UniProtKB

GO - Biological processi

  1. adenohypophysis development Source: MGI
  2. aging Source: Ensembl
  3. ammonium transmembrane transport Source: MGI
  4. cation transmembrane transport Source: MGI
  5. dopamine biosynthetic process Source: MGI
  6. dopamine catabolic process Source: MGI
  7. dopamine transport Source: MGI
  8. lactation Source: MGI
  9. locomotory behavior Source: MGI
  10. monoamine transport Source: MGI
  11. positive regulation of multicellular organism growth Source: MGI
  12. prepulse inhibition Source: MGI
  13. regulation of dopamine metabolic process Source: MGI
  14. response to cAMP Source: Ensembl
  15. response to cocaine Source: MGI
  16. response to drug Source: Ensembl
  17. response to ethanol Source: Ensembl
  18. response to iron ion Source: Ensembl
  19. response to nicotine Source: Ensembl
  20. sensory perception of smell Source: MGI
Complete GO annotation...

Keywords - Biological processi

Neurotransmitter transport, Symport, Transport

Enzyme and pathway databases

ReactomeiREACT_307956. Na+/Cl- dependent neurotransmitter transporters.
REACT_330874. Dopamine clearance from the synaptic cleft.

Names & Taxonomyi

Protein namesi
Recommended name:
Sodium-dependent dopamine transporter
Short name:
DA transporter
Short name:
DAT
Alternative name(s):
Solute carrier family 6 member 3
Gene namesi
Name:Slc6a3
Synonyms:Dat, Dat1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 13

Organism-specific databases

MGIiMGI:94862. Slc6a3.

Subcellular locationi

Cell membrane By similarity; Multi-pass membrane protein By similarity

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 6868CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei69 – 8921Helical; Name=1Sequence AnalysisAdd
BLAST
Transmembranei96 – 11621Helical; Name=2Sequence AnalysisAdd
BLAST
Transmembranei140 – 16021Helical; Name=3Sequence AnalysisAdd
BLAST
Topological domaini161 – 23676ExtracellularSequence AnalysisAdd
BLAST
Transmembranei237 – 25519Helical; Name=4Sequence AnalysisAdd
BLAST
Transmembranei264 – 28118Helical; Name=5Sequence AnalysisAdd
BLAST
Transmembranei317 – 33418Helical; Name=6Sequence AnalysisAdd
BLAST
Transmembranei346 – 36722Helical; Name=7Sequence AnalysisAdd
BLAST
Transmembranei400 – 41920Helical; Name=8Sequence AnalysisAdd
BLAST
Transmembranei446 – 46419Helical; Name=9Sequence AnalysisAdd
BLAST
Transmembranei480 – 50021Helical; Name=10Sequence AnalysisAdd
BLAST
Transmembranei521 – 54020Helical; Name=11Sequence AnalysisAdd
BLAST
Transmembranei559 – 57719Helical; Name=12Sequence AnalysisAdd
BLAST
Topological domaini578 – 61942CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. axon Source: MGI
  2. integral component of plasma membrane Source: InterPro
  3. neuronal cell body Source: MGI
  4. plasma membrane Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 619619Sodium-dependent dopamine transporterPRO_0000214753Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi181 – 1811N-linked (GlcNAc...)Sequence Analysis
Glycosylationi188 – 1881N-linked (GlcNAc...)Sequence Analysis
Glycosylationi196 – 1961N-linked (GlcNAc...)Sequence Analysis
Glycosylationi204 – 2041N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi305 – 305InterchainBy similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiQ61327.
PaxDbiQ61327.
PRIDEiQ61327.

PTM databases

PhosphoSiteiQ61327.

Expressioni

Tissue specificityi

Found in the substantia nigra and ventral tegmental dopamine neurons, in fibers of the medial forebrain bundle ascending into the striatum, and within dense fiber networks and varicosities in the dorsal and ventral striatum. Lower expression in the cortex. Absent from the corpus callosum.1 Publication

Gene expression databases

BgeeiQ61327.
GenevestigatoriQ61327.

Interactioni

Subunit structurei

Homooligomer; disulfide-linked. Interacts with PRKCABP and TGFB1I1. Interacts (via N-terminus) with SYNGR3 (via N-terminus). Interacts with SLC18A2. Interacts with TOR1A (ATP-bound); TOR1A regulates SLC6A3 subcellular location.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
SNCAP378405EBI-7839708,EBI-985879From a different organism.
SncaO550425EBI-7839708,EBI-2310271

Protein-protein interaction databases

BioGridi199053. 2 interactions.
DIPiDIP-41828N.
IntActiQ61327. 2 interactions.
MINTiMINT-576974.

Structurei

3D structure databases

ProteinModelPortaliQ61327.
SMRiQ61327. Positions 59-599.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni560 – 58930Interaction with TGFB1I1By similarityAdd
BLAST

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0733.
GeneTreeiENSGT00760000118857.
HOGENOMiHOG000116406.
HOVERGENiHBG071421.
InParanoidiQ61327.
KOiK05036.
OMAiMTLESMP.
OrthoDBiEOG793B71.
PhylomeDBiQ61327.
TreeFamiTF343812.

Family and domain databases

InterProiIPR000175. Na/ntran_symport.
IPR002436. Na/ntran_symport_dopamine.
[Graphical view]
PANTHERiPTHR11616. PTHR11616. 1 hit.
PfamiPF00209. SNF. 1 hit.
[Graphical view]
PRINTSiPR01202. DOPTRANSPORT.
PR00176. NANEUSMPORT.
PROSITEiPS00610. NA_NEUROTRAN_SYMP_1. 1 hit.
PS00754. NA_NEUROTRAN_SYMP_2. 1 hit.
PS50267. NA_NEUROTRAN_SYMP_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q61327-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKSKCSVGP MSSVVAPAKE PNAVGPREVE LILVKEQNGV QLTNSTLINP
60 70 80 90 100
PQTPVEVQER ETWSKKIDFL LSVIGFAVDL ANVWRFPYLC YKNGGGAFLV
110 120 130 140 150
PYLLFMVIAG MPLFYMELAL GQFNREGAAG VWKICPVLKG VGFTVILISF
160 170 180 190 200
YVGFFYNVII AWALHYFFSS FTMDLPWIHC NNTWNSPNCS DAHSSNSSDG
210 220 230 240 250
LGLNDTFGTT PAAEYFERGV LHLHQSRGID DLGPPRWQLT ACLVLVIVLL
260 270 280 290 300
YFSLWKGVKT SGKVVWITAT MPYVVLTALL LRGVTLPGAM DGIRAYLSVD
310 320 330 340 350
FYRLCEASVW IDAATQVCFS LGVGFGVLIA FSSYNKFTNN CYRDAIITTS
360 370 380 390 400
INSLTSFSSG FVVFSFLGYM AQKHNVPIRD VATDGPGLIF IIYPEAIATL
410 420 430 440 450
PLSSAWAAVF FLMLLTLGID SAMGGMESVI TGLVDEFQLL HRHRELFTLG
460 470 480 490 500
IVLATFLLSL FCVTNGGIYV FTLLDHFAAG TSILFGVLIE AIGVAWFYGV
510 520 530 540 550
QQFSDDIKQM TGQRPNLYWR LCWKLVSPCF LLYVVVVSIV TFRPPHYGAY
560 570 580 590 600
IFPDWANALG WIIATSSMAM VPIYATYKFC SLPGSFREKL AYAITPEKDR
610
QLVDRGEVRQ FTLRHWLLV
Length:619
Mass (Da):68,805
Last modified:May 26, 2002 - v2
Checksum:iF436137BCE461E9B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF109072 mRNA. Translation: AAD19643.1.
BC054119 mRNA. Translation: AAH54119.1.
AH003328 Genomic DNA. Translation: AAA86462.2.
U16265 mRNA. Translation: AAC52283.1.
CCDSiCCDS26632.1.
RefSeqiNP_034150.1. NM_010020.3.
XP_006517145.1. XM_006517082.2.
UniGeneiMm.41993.

Genome annotation databases

EnsembliENSMUST00000022100; ENSMUSP00000022100; ENSMUSG00000021609.
GeneIDi13162.
KEGGimmu:13162.
UCSCiuc007rdn.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF109072 mRNA. Translation: AAD19643.1.
BC054119 mRNA. Translation: AAH54119.1.
AH003328 Genomic DNA. Translation: AAA86462.2.
U16265 mRNA. Translation: AAC52283.1.
CCDSiCCDS26632.1.
RefSeqiNP_034150.1. NM_010020.3.
XP_006517145.1. XM_006517082.2.
UniGeneiMm.41993.

3D structure databases

ProteinModelPortaliQ61327.
SMRiQ61327. Positions 59-599.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi199053. 2 interactions.
DIPiDIP-41828N.
IntActiQ61327. 2 interactions.
MINTiMINT-576974.

Chemistry

BindingDBiQ61327.
ChEMBLiCHEMBL2799.

PTM databases

PhosphoSiteiQ61327.

Proteomic databases

MaxQBiQ61327.
PaxDbiQ61327.
PRIDEiQ61327.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000022100; ENSMUSP00000022100; ENSMUSG00000021609.
GeneIDi13162.
KEGGimmu:13162.
UCSCiuc007rdn.1. mouse.

Organism-specific databases

CTDi6531.
MGIiMGI:94862. Slc6a3.

Phylogenomic databases

eggNOGiCOG0733.
GeneTreeiENSGT00760000118857.
HOGENOMiHOG000116406.
HOVERGENiHBG071421.
InParanoidiQ61327.
KOiK05036.
OMAiMTLESMP.
OrthoDBiEOG793B71.
PhylomeDBiQ61327.
TreeFamiTF343812.

Enzyme and pathway databases

ReactomeiREACT_307956. Na+/Cl- dependent neurotransmitter transporters.
REACT_330874. Dopamine clearance from the synaptic cleft.

Miscellaneous databases

NextBioi283234.
PROiQ61327.
SOURCEiSearch...

Gene expression databases

BgeeiQ61327.
GenevestigatoriQ61327.

Family and domain databases

InterProiIPR000175. Na/ntran_symport.
IPR002436. Na/ntran_symport_dopamine.
[Graphical view]
PANTHERiPTHR11616. PTHR11616. 1 hit.
PfamiPF00209. SNF. 1 hit.
[Graphical view]
PRINTSiPR01202. DOPTRANSPORT.
PR00176. NANEUSMPORT.
PROSITEiPS00610. NA_NEUROTRAN_SYMP_1. 1 hit.
PS00754. NA_NEUROTRAN_SYMP_2. 1 hit.
PS50267. NA_NEUROTRAN_SYMP_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning of the mouse dopamine transporter and pharmacological comparison with the human homologue."
    Wu X., Gu H.H.
    Gene 233:163-170(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: C57BL/6J.
    Tissue: Brain.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain.
  3. "Human and mouse dopamine transporter genes: conservation of 5'-flanking sequence elements and gene structures."
    Donovan D.M., Vandenbergh D.J., Perry M.P., Bird G.S., Ingersoll R., Nanthakumar E., Uhl G.R.
    Brain Res. Mol. Brain Res. 30:327-335(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-343.
    Strain: BALB/c.
    Tissue: Brain.
  4. "The multiple LIM domain-containing adaptor protein Hic-5 synaptically colocalizes and interacts with the dopamine transporter."
    Carneiro A.M.D., Ingram S.L., Beaulieu J.-M., Sweeney A., Amara S.G., Thomas S.M., Caron M.G., Torres G.E.
    J. Neurosci. 22:7045-7054(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH TGFB1I1.
  5. "Physical and functional interaction between the dopamine transporter and the synaptic vesicle protein synaptogyrin-3."
    Egana L.A., Cuevas R.A., Baust T.B., Parra L.A., Leak R.K., Hochendoner S., Pena K., Quiroz M., Hong W.C., Dorostkar M.M., Janz R., Sitte H.H., Torres G.E.
    J. Neurosci. 29:4592-4604(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH SYNGR3 AND SLC18A2, TISSUE SPECIFICITY.

Entry informationi

Entry nameiSC6A3_MOUSE
AccessioniPrimary (citable) accession number: Q61327
Secondary accession number(s): Q60719, Q9R1I2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 31, 1997
Last sequence update: May 26, 2002
Last modified: March 31, 2015
This is version 135 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

This protein is the target of psychomotor stimulants such as amphetamines and cocaine.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.