Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q61327

- SC6A3_MOUSE

UniProt

Q61327 - SC6A3_MOUSE

Protein

Sodium-dependent dopamine transporter

Gene

Slc6a3

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 129 (01 Oct 2014)
      Sequence version 2 (27 May 2002)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Amine transporter. Terminates the action of dopamine by its high affinity sodium-dependent reuptake into presynaptic terminals By similarity.By similarity

    GO - Molecular functioni

    1. dopamine:sodium symporter activity Source: Ensembl
    2. dopamine binding Source: MGI
    3. dopamine transmembrane transporter activity Source: MGI
    4. drug binding Source: Ensembl
    5. monoamine transmembrane transporter activity Source: MGI
    6. protein binding Source: IntAct
    7. protein N-terminus binding Source: UniProtKB

    GO - Biological processi

    1. adenohypophysis development Source: MGI
    2. aging Source: Ensembl
    3. dopamine biosynthetic process Source: MGI
    4. dopamine catabolic process Source: MGI
    5. dopamine transport Source: MGI
    6. lactation Source: MGI
    7. locomotory behavior Source: MGI
    8. monoamine transport Source: MGI
    9. positive regulation of multicellular organism growth Source: MGI
    10. prepulse inhibition Source: MGI
    11. regulation of dopamine metabolic process Source: MGI
    12. response to cAMP Source: Ensembl
    13. response to cocaine Source: MGI
    14. response to drug Source: Ensembl
    15. response to ethanol Source: Ensembl
    16. response to iron ion Source: Ensembl
    17. response to nicotine Source: Ensembl
    18. sensory perception of smell Source: MGI

    Keywords - Biological processi

    Neurotransmitter transport, Symport, Transport

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Sodium-dependent dopamine transporter
    Short name:
    DA transporter
    Short name:
    DAT
    Alternative name(s):
    Solute carrier family 6 member 3
    Gene namesi
    Name:Slc6a3
    Synonyms:Dat, Dat1
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 13

    Organism-specific databases

    MGIiMGI:94862. Slc6a3.

    Subcellular locationi

    Cell membrane By similarity; Multi-pass membrane protein By similarity

    GO - Cellular componenti

    1. axon Source: MGI
    2. integral component of plasma membrane Source: InterPro
    3. neuronal cell body Source: Ensembl

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 619619Sodium-dependent dopamine transporterPRO_0000214753Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi181 – 1811N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi188 – 1881N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi196 – 1961N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi204 – 2041N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi305 – 305InterchainBy similarity

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    PaxDbiQ61327.
    PRIDEiQ61327.

    PTM databases

    PhosphoSiteiQ61327.

    Expressioni

    Tissue specificityi

    Found in the substantia nigra and ventral tegmental dopamine neurons, in fibers of the medial forebrain bundle ascending into the striatum, and within dense fiber networks and varicosities in the dorsal and ventral striatum. Lower expression in the cortex. Absent from the corpus callosum.1 Publication

    Gene expression databases

    BgeeiQ61327.
    GenevestigatoriQ61327.

    Interactioni

    Subunit structurei

    Homooligomer; disulfide-linked. Interacts with PRKCABP and TGFB1I1. Interacts (via N-terminus) with SYNGR3 (via N-terminus). Interacts with SLC18A2. Interacts with TOR1A (ATP-bound); TOR1A regulates SLC6A3 subcellular location.2 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    SNCAP378405EBI-7839708,EBI-985879From a different organism.
    SncaO550425EBI-7839708,EBI-2310271

    Protein-protein interaction databases

    BioGridi199053. 2 interactions.
    DIPiDIP-41828N.
    IntActiQ61327. 2 interactions.
    MINTiMINT-576974.

    Structurei

    3D structure databases

    ProteinModelPortaliQ61327.
    SMRiQ61327. Positions 59-599.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 6868CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini161 – 23676ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini578 – 61942CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei69 – 8921Helical; Name=1Sequence AnalysisAdd
    BLAST
    Transmembranei96 – 11621Helical; Name=2Sequence AnalysisAdd
    BLAST
    Transmembranei140 – 16021Helical; Name=3Sequence AnalysisAdd
    BLAST
    Transmembranei237 – 25519Helical; Name=4Sequence AnalysisAdd
    BLAST
    Transmembranei264 – 28118Helical; Name=5Sequence AnalysisAdd
    BLAST
    Transmembranei317 – 33418Helical; Name=6Sequence AnalysisAdd
    BLAST
    Transmembranei346 – 36722Helical; Name=7Sequence AnalysisAdd
    BLAST
    Transmembranei400 – 41920Helical; Name=8Sequence AnalysisAdd
    BLAST
    Transmembranei446 – 46419Helical; Name=9Sequence AnalysisAdd
    BLAST
    Transmembranei480 – 50021Helical; Name=10Sequence AnalysisAdd
    BLAST
    Transmembranei521 – 54020Helical; Name=11Sequence AnalysisAdd
    BLAST
    Transmembranei559 – 57719Helical; Name=12Sequence AnalysisAdd
    BLAST

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni560 – 58930Interaction with TGFB1I1By similarityAdd
    BLAST

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG0733.
    GeneTreeiENSGT00740000115174.
    HOGENOMiHOG000116406.
    HOVERGENiHBG071421.
    InParanoidiQ61327.
    KOiK05036.
    OMAiIFPDWAN.
    OrthoDBiEOG793B71.
    PhylomeDBiQ61327.
    TreeFamiTF343812.

    Family and domain databases

    InterProiIPR000175. Na/ntran_symport.
    IPR002436. Na/ntran_symport_dopamine.
    [Graphical view]
    PANTHERiPTHR11616. PTHR11616. 1 hit.
    PfamiPF00209. SNF. 1 hit.
    [Graphical view]
    PRINTSiPR01202. DOPTRANSPORT.
    PR00176. NANEUSMPORT.
    PROSITEiPS00610. NA_NEUROTRAN_SYMP_1. 1 hit.
    PS00754. NA_NEUROTRAN_SYMP_2. 1 hit.
    PS50267. NA_NEUROTRAN_SYMP_3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q61327-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSKSKCSVGP MSSVVAPAKE PNAVGPREVE LILVKEQNGV QLTNSTLINP    50
    PQTPVEVQER ETWSKKIDFL LSVIGFAVDL ANVWRFPYLC YKNGGGAFLV 100
    PYLLFMVIAG MPLFYMELAL GQFNREGAAG VWKICPVLKG VGFTVILISF 150
    YVGFFYNVII AWALHYFFSS FTMDLPWIHC NNTWNSPNCS DAHSSNSSDG 200
    LGLNDTFGTT PAAEYFERGV LHLHQSRGID DLGPPRWQLT ACLVLVIVLL 250
    YFSLWKGVKT SGKVVWITAT MPYVVLTALL LRGVTLPGAM DGIRAYLSVD 300
    FYRLCEASVW IDAATQVCFS LGVGFGVLIA FSSYNKFTNN CYRDAIITTS 350
    INSLTSFSSG FVVFSFLGYM AQKHNVPIRD VATDGPGLIF IIYPEAIATL 400
    PLSSAWAAVF FLMLLTLGID SAMGGMESVI TGLVDEFQLL HRHRELFTLG 450
    IVLATFLLSL FCVTNGGIYV FTLLDHFAAG TSILFGVLIE AIGVAWFYGV 500
    QQFSDDIKQM TGQRPNLYWR LCWKLVSPCF LLYVVVVSIV TFRPPHYGAY 550
    IFPDWANALG WIIATSSMAM VPIYATYKFC SLPGSFREKL AYAITPEKDR 600
    QLVDRGEVRQ FTLRHWLLV 619
    Length:619
    Mass (Da):68,805
    Last modified:May 27, 2002 - v2
    Checksum:iF436137BCE461E9B
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF109072 mRNA. Translation: AAD19643.1.
    BC054119 mRNA. Translation: AAH54119.1.
    AH003328 Genomic DNA. Translation: AAA86462.2.
    U16265 mRNA. Translation: AAC52283.1.
    CCDSiCCDS26632.1.
    RefSeqiNP_034150.1. NM_010020.3.
    XP_006517145.1. XM_006517082.1.
    UniGeneiMm.41993.

    Genome annotation databases

    EnsembliENSMUST00000022100; ENSMUSP00000022100; ENSMUSG00000021609.
    GeneIDi13162.
    KEGGimmu:13162.
    UCSCiuc007rdn.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF109072 mRNA. Translation: AAD19643.1 .
    BC054119 mRNA. Translation: AAH54119.1 .
    AH003328 Genomic DNA. Translation: AAA86462.2 .
    U16265 mRNA. Translation: AAC52283.1 .
    CCDSi CCDS26632.1.
    RefSeqi NP_034150.1. NM_010020.3.
    XP_006517145.1. XM_006517082.1.
    UniGenei Mm.41993.

    3D structure databases

    ProteinModelPortali Q61327.
    SMRi Q61327. Positions 59-599.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 199053. 2 interactions.
    DIPi DIP-41828N.
    IntActi Q61327. 2 interactions.
    MINTi MINT-576974.

    Chemistry

    BindingDBi Q61327.
    ChEMBLi CHEMBL2799.

    PTM databases

    PhosphoSitei Q61327.

    Proteomic databases

    PaxDbi Q61327.
    PRIDEi Q61327.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000022100 ; ENSMUSP00000022100 ; ENSMUSG00000021609 .
    GeneIDi 13162.
    KEGGi mmu:13162.
    UCSCi uc007rdn.1. mouse.

    Organism-specific databases

    CTDi 6531.
    MGIi MGI:94862. Slc6a3.

    Phylogenomic databases

    eggNOGi COG0733.
    GeneTreei ENSGT00740000115174.
    HOGENOMi HOG000116406.
    HOVERGENi HBG071421.
    InParanoidi Q61327.
    KOi K05036.
    OMAi IFPDWAN.
    OrthoDBi EOG793B71.
    PhylomeDBi Q61327.
    TreeFami TF343812.

    Miscellaneous databases

    NextBioi 283234.
    PROi Q61327.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q61327.
    Genevestigatori Q61327.

    Family and domain databases

    InterProi IPR000175. Na/ntran_symport.
    IPR002436. Na/ntran_symport_dopamine.
    [Graphical view ]
    PANTHERi PTHR11616. PTHR11616. 1 hit.
    Pfami PF00209. SNF. 1 hit.
    [Graphical view ]
    PRINTSi PR01202. DOPTRANSPORT.
    PR00176. NANEUSMPORT.
    PROSITEi PS00610. NA_NEUROTRAN_SYMP_1. 1 hit.
    PS00754. NA_NEUROTRAN_SYMP_2. 1 hit.
    PS50267. NA_NEUROTRAN_SYMP_3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning of the mouse dopamine transporter and pharmacological comparison with the human homologue."
      Wu X., Gu H.H.
      Gene 233:163-170(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: C57BL/6J.
      Tissue: Brain.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Brain.
    3. "Human and mouse dopamine transporter genes: conservation of 5'-flanking sequence elements and gene structures."
      Donovan D.M., Vandenbergh D.J., Perry M.P., Bird G.S., Ingersoll R., Nanthakumar E., Uhl G.R.
      Brain Res. Mol. Brain Res. 30:327-335(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-343.
      Strain: BALB/c.
      Tissue: Brain.
    4. "The multiple LIM domain-containing adaptor protein Hic-5 synaptically colocalizes and interacts with the dopamine transporter."
      Carneiro A.M.D., Ingram S.L., Beaulieu J.-M., Sweeney A., Amara S.G., Thomas S.M., Caron M.G., Torres G.E.
      J. Neurosci. 22:7045-7054(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH TGFB1I1.
    5. "Physical and functional interaction between the dopamine transporter and the synaptic vesicle protein synaptogyrin-3."
      Egana L.A., Cuevas R.A., Baust T.B., Parra L.A., Leak R.K., Hochendoner S., Pena K., Quiroz M., Hong W.C., Dorostkar M.M., Janz R., Sitte H.H., Torres G.E.
      J. Neurosci. 29:4592-4604(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH SYNGR3 AND SLC18A2, TISSUE SPECIFICITY.

    Entry informationi

    Entry nameiSC6A3_MOUSE
    AccessioniPrimary (citable) accession number: Q61327
    Secondary accession number(s): Q60719, Q9R1I2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: May 27, 2002
    Last modified: October 1, 2014
    This is version 129 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    This protein is the target of psychomotor stimulants such as amphetamines and cocaine.

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3