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Q61321 (SIX4_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 131. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Homeobox protein SIX4
Alternative name(s):
Sine oculis homeobox homolog 4
Skeletal muscle-specific ARE-binding protein AREC3
Gene names
Name:Six4
Synonyms:Arec3
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length775 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in skeletal muscle development. Also implicated in retina and kidney development.

Subcellular location

Cytoplasm. Nucleus.

Tissue specificity

Mainly expressed in the skeletal muscle (isoform 1 and isoform 2 but not isoform 3) and weakly in the heart. Also found in the retina and the distal tube of kidney.

Sequence similarities

Belongs to the SIX/Sine oculis homeobox family.

Contains 1 homeobox DNA-binding domain.

Ontologies

Keywords
   Cellular componentCytoplasm
Nucleus
   Coding sequence diversityAlternative splicing
   DomainHomeobox
   LigandDNA-binding
   Molecular functionDevelopmental protein
   PTMAcetylation
Phosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processanatomical structure development

Inferred from genetic interaction PubMed 16530750. Source: MGI

embryonic cranial skeleton morphogenesis

Inferred from genetic interaction PubMed 20515681. Source: MGI

embryonic skeletal system morphogenesis

Inferred from genetic interaction PubMed 15788460. Source: MGI

generation of neurons

Inferred from mutant phenotype PubMed 16938278. Source: UniProtKB

inner ear morphogenesis

Inferred from genetic interaction PubMed 15788460. Source: MGI

metanephric mesenchyme development

Inferred from mutant phenotype PubMed 17300925. Source: UniProtKB

myoblast migration

Inferred from genetic interaction PubMed 15788460. Source: MGI

negative regulation of neuron apoptotic process

Inferred from mutant phenotype PubMed 16938278. Source: UniProtKB

positive regulation of branching involved in ureteric bud morphogenesis

Inferred from mutant phenotype PubMed 17300925. Source: UniProtKB

positive regulation of transcription from RNA polymerase II promoter

Inferred from direct assay PubMed 15955062. Source: MGI

positive regulation of transcription, DNA-templated

Inferred from mutant phenotype PubMed 17300925. Source: UniProtKB

positive regulation of ureteric bud formation

Inferred from mutant phenotype PubMed 17300925. Source: UniProtKB

regulation of branch elongation involved in ureteric bud branching

Inferred from mutant phenotype PubMed 17300925. Source: UniProtKB

regulation of gene expression

Inferred from genetic interaction PubMed 21884692. Source: MGI

regulation of protein localization

Inferred from genetic interaction PubMed 21884692. Source: MGI

regulation of synaptic growth at neuromuscular junction

Inferred from genetic interaction PubMed 21884692. Source: MGI

skeletal muscle tissue development

Inferred from genetic interaction PubMed 15788460. Source: MGI

thymus development

Inferred from genetic interaction PubMed 16530750. Source: MGI

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

nucleus

Inferred from direct assay PubMed 8814301PubMed 9826681. Source: MGI

   Molecular_functionDNA binding

Inferred from direct assay PubMed 14966291. Source: MGI

protein binding

Inferred from physical interaction PubMed 20300060. Source: IntAct

sequence-specific DNA binding

Inferred from direct assay PubMed 8814301PubMed 9826681. Source: MGI

sequence-specific DNA binding transcription factor activity

Inferred from direct assay PubMed 14966291PubMed 15955062. Source: MGI

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Kdm6aO705462EBI-986524,EBI-1573712

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Note: Additional isoforms seem to exist.
Isoform 1 (identifier: Q61321-1)

Also known as: SM;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q61321-2)

Also known as: M18;

The sequence of this isoform differs from the canonical sequence as follows:
     2-9: SSSSPTGQ → QKAAIRLHYFALAAILM
     37-100: Missing.
Note: Incomplete sequence.
Isoform 3 (identifier: Q61321-3)

Also known as: M8;

The sequence of this isoform differs from the canonical sequence as follows:
     2-9: SSSSPTGQ → QKAAIRLHYFALAAILM
     188-319: ERARGRPLGA...DGVTNLSLSS → AGNSPCPAPS...CNKLEMLRYH
     320-775: Missing.
Note: Incomplete sequence.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 775774Homeobox protein SIX4
PRO_0000049304

Regions

DNA binding216 – 27560Homeobox
Region582 – 775194Transactivation domain
Compositional bias2 – 54Poly-Ser
Compositional bias58 – 614Poly-Ala
Compositional bias70 – 767Poly-Ala
Compositional bias92 – 954Poly-Ala

Amino acid modifications

Modified residue21N-acetylserine By similarity
Modified residue6341Phosphoserine By similarity

Natural variations

Alternative sequence2 – 98SSSSPTGQ → QKAAIRLHYFALAAILM in isoform 2 and isoform 3.
VSP_002293
Alternative sequence37 – 10064Missing in isoform 2.
VSP_002294
Alternative sequence188 – 319132ERARG…LSLSS → AGNSPCPAPSGTARRRCIVS RRSRATRSRSSTSRIATPRR LRSGTWPRSPASPSPRSATG SRTGGSVTETPPRPSPKANR MATPVPRMNPARDMRICLLI HFQAHLMASPTSASLATWSQ YICNKLEMLRYH in isoform 3.
VSP_002295
Alternative sequence320 – 775456Missing in isoform 3.
VSP_002296

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 (SM) [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: B06EBB64E04E5061

FASTA77582,263
        10         20         30         40         50         60 
MSSSSPTGQI ASAADIKQEN GMESASEGQE AHREVAGGAA AGLSPPAPAP FPLEPGDAAA 

        70         80         90        100        110        120 
ASRVSREEGA AAAGAADQVQ LHSELLGRHQ HAAAAQPPLA FSPDHVACVC EALQQGGNLD 

       130        140        150        160        170        180 
RLARFLWSLP QSDLLRGNES LLKARALVAF HQGIYPELYS ILESHSFESA NHPLLQQLWY 

       190        200        210        220        230        240 
KARYTEAERA RGRPLGAVDK YRLRRKFPLP RTIWDGEETV YCFKEKSRNA LKELYKQNRY 

       250        260        270        280        290        300 
PSPAEKRHLA KITGLSLTQV SNWFKNRRQR DRNPSETQSK SESDGNPSTE DESSKGHEDL 

       310        320        330        340        350        360 
SPHPLSGASD GVTNLSLSSH VEPVYMQQIG NAKISLSSSG VLLNGSLVPA STSPVFLNGN 

       370        380        390        400        410        420 
SFIQGHNGVI LNGLNVGNTQ TVSLNPPKMS SNIVGNGIAM TDILGSTSQD VKEFKVLQSS 

       430        440        450        460        470        480 
AVNSAATTSY SPSAPVSFPG LIPCTEVKRE GIQTVASQDG GSVVTFTTPV QINQYGIVQI 

       490        500        510        520        530        540 
PNSGANGQFL NGSIGFSPLQ LPPVSVAASQ GNLSVTPSTS DGSTFTSEPA TVQHGKLFLS 

       550        560        570        580        590        600 
PLTPSAVVYT VPNSGQTVGA VKQEGLERGL VFSQLMPVNH SAQVNASLSS ENLSGSGLHP 

       610        620        630        640        650        660 
LTSSLVNVSA AHGFSLTPPT LLNPTELNPD LAESQPVSAP VASKCTVSSV SNTNYATLQN 

       670        680        690        700        710        720 
CSLIPGQDLL SGPMTQAALG EIVPTAEEQV SHASTAVHQD FVREQRLVLQ SVPNIKENFL 

       730        740        750        760        770 
QNSENKATNN LMMLDSKSKY VLDGMVEAGC EDLGTDKKEL AKLQTVQLDE DMQDL 

« Hide

Isoform 2 (M18) [UniParc].

Checksum: F893580411E05927
Show »

FASTA72077,334
Isoform 3 (M8) [UniParc].

Checksum: EE7E9E97952C03EB
Show »

FASTA32835,344

References

« Hide 'large scale' references
[1]"Structure, function and expression of a murine homeobox protein AREC3, a homologue of Drosophila sine oculis gene product, and implication in development."
Kawakami K., Ohto H., Ikeda K., Roeder R.G.
Nucleic Acids Res. 24:303-310(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), PARTIAL NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 2 AND 3).
Strain: BALB/c.
Tissue: Myoblast and Skeletal muscle.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Brain.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D50416 mRNA. Translation: BAA08915.1.
D50417 mRNA. Translation: BAA08916.1.
D50418 mRNA. Translation: BAA08917.1.
BC137931 mRNA. Translation: AAI37932.1.
BC137934 mRNA. Translation: AAI37935.1.
CCDSCCDS25974.1. [Q61321-1]
PIRS63626.
S63628.
S63629.
RefSeqNP_035512.1. NM_011382.2. [Q61321-1]
UniGeneMm.249575.

3D structure databases

ProteinModelPortalQ61321.
SMRQ61321. Positions 97-272.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActQ61321. 2 interactions.
MINTMINT-7949316.

PTM databases

PhosphoSiteQ61321.

Proteomic databases

PRIDEQ61321.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000043208; ENSMUSP00000036150; ENSMUSG00000034460. [Q61321-1]
GeneID20474.
KEGGmmu:20474.
UCSCuc007nwb.1. mouse. [Q61321-1]

Organism-specific databases

CTD51804.
MGIMGI:106034. Six4.

Phylogenomic databases

eggNOGNOG244874.
GeneTreeENSGT00540000070251.
HOGENOMHOG000261651.
HOVERGENHBG017802.
InParanoidB2RQH3.
KOK15615.
OMAIKQENGM.
OrthoDBEOG7C5M8Z.
PhylomeDBQ61321.
TreeFamTF315545.

Gene expression databases

BgeeQ61321.
GenevestigatorQ61321.

Family and domain databases

Gene3D1.10.10.60. 1 hit.
InterProIPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR009057. Homeodomain-like.
[Graphical view]
PfamPF00046. Homeobox. 1 hit.
[Graphical view]
SMARTSM00389. HOX. 1 hit.
[Graphical view]
SUPFAMSSF46689. SSF46689. 1 hit.
PROSITEPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio298591.
PROQ61321.
SOURCESearch...

Entry information

Entry nameSIX4_MOUSE
AccessionPrimary (citable) accession number: Q61321
Secondary accession number(s): B2RQH3, Q61322, Q61323
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: July 9, 2014
This is version 131 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot