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Q61321

- SIX4_MOUSE

UniProt

Q61321 - SIX4_MOUSE

Protein

Homeobox protein SIX4

Gene

Six4

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 132 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Involved in skeletal muscle development. Also implicated in retina and kidney development.

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    DNA bindingi216 – 27560HomeoboxPROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. DNA binding Source: MGI
    2. protein binding Source: IntAct
    3. sequence-specific DNA binding Source: MGI
    4. sequence-specific DNA binding transcription factor activity Source: MGI

    GO - Biological processi

    1. anatomical structure development Source: MGI
    2. embryonic cranial skeleton morphogenesis Source: MGI
    3. embryonic skeletal system morphogenesis Source: MGI
    4. generation of neurons Source: UniProtKB
    5. inner ear morphogenesis Source: MGI
    6. metanephric mesenchyme development Source: UniProtKB
    7. myoblast migration Source: MGI
    8. negative regulation of neuron apoptotic process Source: UniProtKB
    9. positive regulation of branching involved in ureteric bud morphogenesis Source: UniProtKB
    10. positive regulation of transcription, DNA-templated Source: UniProtKB
    11. positive regulation of transcription from RNA polymerase II promoter Source: MGI
    12. positive regulation of ureteric bud formation Source: UniProtKB
    13. regulation of branch elongation involved in ureteric bud branching Source: UniProtKB
    14. regulation of gene expression Source: MGI
    15. regulation of protein localization Source: MGI
    16. regulation of synaptic growth at neuromuscular junction Source: MGI
    17. skeletal muscle tissue development Source: MGI
    18. thymus development Source: MGI

    Keywords - Molecular functioni

    Developmental protein

    Keywords - Ligandi

    DNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Homeobox protein SIX4
    Alternative name(s):
    Sine oculis homeobox homolog 4
    Skeletal muscle-specific ARE-binding protein AREC3
    Gene namesi
    Name:Six4
    Synonyms:Arec3
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 12

    Organism-specific databases

    MGIiMGI:106034. Six4.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell
    2. nucleus Source: MGI

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 775774Homeobox protein SIX4PRO_0000049304Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylserineBy similarity
    Modified residuei634 – 6341PhosphoserineBy similarity

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    PRIDEiQ61321.

    PTM databases

    PhosphoSiteiQ61321.

    Expressioni

    Tissue specificityi

    Mainly expressed in the skeletal muscle (isoform 1 and isoform 2 but not isoform 3), and weakly in the heart. Also found in the retina and the distal tube of kidney.

    Gene expression databases

    BgeeiQ61321.
    GenevestigatoriQ61321.

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    Kdm6aO705462EBI-986524,EBI-1573712

    Protein-protein interaction databases

    IntActiQ61321. 2 interactions.
    MINTiMINT-7949316.

    Structurei

    3D structure databases

    ProteinModelPortaliQ61321.
    SMRiQ61321. Positions 97-272.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni582 – 775194Transactivation domainAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi2 – 54Poly-Ser
    Compositional biasi58 – 614Poly-Ala
    Compositional biasi70 – 767Poly-Ala
    Compositional biasi92 – 954Poly-Ala

    Sequence similaritiesi

    Belongs to the SIX/Sine oculis homeobox family.Curated
    Contains 1 homeobox DNA-binding domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Homeobox

    Phylogenomic databases

    eggNOGiNOG244874.
    GeneTreeiENSGT00540000070251.
    HOGENOMiHOG000261651.
    HOVERGENiHBG017802.
    InParanoidiB2RQH3.
    KOiK15615.
    OMAiIKQENGM.
    OrthoDBiEOG7C5M8Z.
    PhylomeDBiQ61321.
    TreeFamiTF315545.

    Family and domain databases

    Gene3Di1.10.10.60. 1 hit.
    InterProiIPR017970. Homeobox_CS.
    IPR001356. Homeobox_dom.
    IPR009057. Homeodomain-like.
    [Graphical view]
    PfamiPF00046. Homeobox. 1 hit.
    [Graphical view]
    SMARTiSM00389. HOX. 1 hit.
    [Graphical view]
    SUPFAMiSSF46689. SSF46689. 1 hit.
    PROSITEiPS00027. HOMEOBOX_1. 1 hit.
    PS50071. HOMEOBOX_2. 1 hit.
    [Graphical view]

    Sequences (3)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 3 isoformsi produced by alternative splicing. Align

    Note: Additional isoforms seem to exist.

    Isoform 1 (identifier: Q61321-1) [UniParc]FASTAAdd to Basket

    Also known as: SM

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MSSSSPTGQI ASAADIKQEN GMESASEGQE AHREVAGGAA AGLSPPAPAP    50
    FPLEPGDAAA ASRVSREEGA AAAGAADQVQ LHSELLGRHQ HAAAAQPPLA 100
    FSPDHVACVC EALQQGGNLD RLARFLWSLP QSDLLRGNES LLKARALVAF 150
    HQGIYPELYS ILESHSFESA NHPLLQQLWY KARYTEAERA RGRPLGAVDK 200
    YRLRRKFPLP RTIWDGEETV YCFKEKSRNA LKELYKQNRY PSPAEKRHLA 250
    KITGLSLTQV SNWFKNRRQR DRNPSETQSK SESDGNPSTE DESSKGHEDL 300
    SPHPLSGASD GVTNLSLSSH VEPVYMQQIG NAKISLSSSG VLLNGSLVPA 350
    STSPVFLNGN SFIQGHNGVI LNGLNVGNTQ TVSLNPPKMS SNIVGNGIAM 400
    TDILGSTSQD VKEFKVLQSS AVNSAATTSY SPSAPVSFPG LIPCTEVKRE 450
    GIQTVASQDG GSVVTFTTPV QINQYGIVQI PNSGANGQFL NGSIGFSPLQ 500
    LPPVSVAASQ GNLSVTPSTS DGSTFTSEPA TVQHGKLFLS PLTPSAVVYT 550
    VPNSGQTVGA VKQEGLERGL VFSQLMPVNH SAQVNASLSS ENLSGSGLHP 600
    LTSSLVNVSA AHGFSLTPPT LLNPTELNPD LAESQPVSAP VASKCTVSSV 650
    SNTNYATLQN CSLIPGQDLL SGPMTQAALG EIVPTAEEQV SHASTAVHQD 700
    FVREQRLVLQ SVPNIKENFL QNSENKATNN LMMLDSKSKY VLDGMVEAGC 750
    EDLGTDKKEL AKLQTVQLDE DMQDL 775
    Length:775
    Mass (Da):82,263
    Last modified:November 1, 1996 - v1
    Checksum:iB06EBB64E04E5061
    GO
    Isoform 2 (identifier: Q61321-2) [UniParc]FASTAAdd to Basket

    Also known as: M18

    The sequence of this isoform differs from the canonical sequence as follows:
         2-9: SSSSPTGQ → QKAAIRLHYFALAAILM
         37-100: Missing.

    Note: Incomplete sequence.

    Show »
    Length:720
    Mass (Da):77,334
    Checksum:iF893580411E05927
    GO
    Isoform 3 (identifier: Q61321-3) [UniParc]FASTAAdd to Basket

    Also known as: M8

    The sequence of this isoform differs from the canonical sequence as follows:
         2-9: SSSSPTGQ → QKAAIRLHYFALAAILM
         188-319: ERARGRPLGA...DGVTNLSLSS → AGNSPCPAPS...CNKLEMLRYH
         320-775: Missing.

    Note: Incomplete sequence.

    Show »
    Length:328
    Mass (Da):35,344
    Checksum:iEE7E9E97952C03EB
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei2 – 98SSSSPTGQ → QKAAIRLHYFALAAILM in isoform 2 and isoform 3. CuratedVSP_002293
    Alternative sequencei37 – 10064Missing in isoform 2. CuratedVSP_002294Add
    BLAST
    Alternative sequencei188 – 319132ERARG…LSLSS → AGNSPCPAPSGTARRRCIVS RRSRATRSRSSTSRIATPRR LRSGTWPRSPASPSPRSATG SRTGGSVTETPPRPSPKANR MATPVPRMNPARDMRICLLI HFQAHLMASPTSASLATWSQ YICNKLEMLRYH in isoform 3. CuratedVSP_002295Add
    BLAST
    Alternative sequencei320 – 775456Missing in isoform 3. CuratedVSP_002296Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D50416 mRNA. Translation: BAA08915.1.
    D50417 mRNA. Translation: BAA08916.1.
    D50418 mRNA. Translation: BAA08917.1.
    BC137931 mRNA. Translation: AAI37932.1.
    BC137934 mRNA. Translation: AAI37935.1.
    CCDSiCCDS25974.1. [Q61321-1]
    PIRiS63626.
    S63628.
    S63629.
    RefSeqiNP_035512.1. NM_011382.2. [Q61321-1]
    UniGeneiMm.249575.

    Genome annotation databases

    EnsembliENSMUST00000043208; ENSMUSP00000036150; ENSMUSG00000034460. [Q61321-1]
    GeneIDi20474.
    KEGGimmu:20474.
    UCSCiuc007nwb.1. mouse. [Q61321-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D50416 mRNA. Translation: BAA08915.1 .
    D50417 mRNA. Translation: BAA08916.1 .
    D50418 mRNA. Translation: BAA08917.1 .
    BC137931 mRNA. Translation: AAI37932.1 .
    BC137934 mRNA. Translation: AAI37935.1 .
    CCDSi CCDS25974.1. [Q61321-1 ]
    PIRi S63626.
    S63628.
    S63629.
    RefSeqi NP_035512.1. NM_011382.2. [Q61321-1 ]
    UniGenei Mm.249575.

    3D structure databases

    ProteinModelPortali Q61321.
    SMRi Q61321. Positions 97-272.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q61321. 2 interactions.
    MINTi MINT-7949316.

    PTM databases

    PhosphoSitei Q61321.

    Proteomic databases

    PRIDEi Q61321.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000043208 ; ENSMUSP00000036150 ; ENSMUSG00000034460 . [Q61321-1 ]
    GeneIDi 20474.
    KEGGi mmu:20474.
    UCSCi uc007nwb.1. mouse. [Q61321-1 ]

    Organism-specific databases

    CTDi 51804.
    MGIi MGI:106034. Six4.

    Phylogenomic databases

    eggNOGi NOG244874.
    GeneTreei ENSGT00540000070251.
    HOGENOMi HOG000261651.
    HOVERGENi HBG017802.
    InParanoidi B2RQH3.
    KOi K15615.
    OMAi IKQENGM.
    OrthoDBi EOG7C5M8Z.
    PhylomeDBi Q61321.
    TreeFami TF315545.

    Miscellaneous databases

    NextBioi 298591.
    PROi Q61321.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q61321.
    Genevestigatori Q61321.

    Family and domain databases

    Gene3Di 1.10.10.60. 1 hit.
    InterProi IPR017970. Homeobox_CS.
    IPR001356. Homeobox_dom.
    IPR009057. Homeodomain-like.
    [Graphical view ]
    Pfami PF00046. Homeobox. 1 hit.
    [Graphical view ]
    SMARTi SM00389. HOX. 1 hit.
    [Graphical view ]
    SUPFAMi SSF46689. SSF46689. 1 hit.
    PROSITEi PS00027. HOMEOBOX_1. 1 hit.
    PS50071. HOMEOBOX_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Structure, function and expression of a murine homeobox protein AREC3, a homologue of Drosophila sine oculis gene product, and implication in development."
      Kawakami K., Ohto H., Ikeda K., Roeder R.G.
      Nucleic Acids Res. 24:303-310(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), PARTIAL NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 2 AND 3).
      Strain: BALB/c.
      Tissue: Myoblast and Skeletal muscle.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Tissue: Brain.

    Entry informationi

    Entry nameiSIX4_MOUSE
    AccessioniPrimary (citable) accession number: Q61321
    Secondary accession number(s): B2RQH3, Q61322, Q61323
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 132 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3