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Q61321

- SIX4_MOUSE

UniProt

Q61321 - SIX4_MOUSE

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Protein

Homeobox protein SIX4

Gene

Six4

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Involved in skeletal muscle development. Also implicated in retina and kidney development.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi216 – 27560HomeoboxPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. DNA binding Source: MGI
  2. sequence-specific DNA binding Source: MGI
  3. sequence-specific DNA binding transcription factor activity Source: MGI

GO - Biological processi

  1. anatomical structure development Source: MGI
  2. embryonic cranial skeleton morphogenesis Source: MGI
  3. embryonic skeletal system morphogenesis Source: MGI
  4. generation of neurons Source: UniProtKB
  5. inner ear morphogenesis Source: MGI
  6. metanephric mesenchyme development Source: UniProtKB
  7. myoblast migration Source: MGI
  8. negative regulation of neuron apoptotic process Source: UniProtKB
  9. positive regulation of branching involved in ureteric bud morphogenesis Source: UniProtKB
  10. positive regulation of transcription, DNA-templated Source: UniProtKB
  11. positive regulation of transcription from RNA polymerase II promoter Source: MGI
  12. positive regulation of ureteric bud formation Source: UniProtKB
  13. regulation of branch elongation involved in ureteric bud branching Source: UniProtKB
  14. regulation of gene expression Source: MGI
  15. regulation of protein localization Source: MGI
  16. regulation of synaptic growth at neuromuscular junction Source: MGI
  17. skeletal muscle tissue development Source: MGI
  18. thymus development Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Homeobox protein SIX4
Alternative name(s):
Sine oculis homeobox homolog 4
Skeletal muscle-specific ARE-binding protein AREC3
Gene namesi
Name:Six4
Synonyms:Arec3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 12

Organism-specific databases

MGIiMGI:106034. Six4.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
  2. nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 775774Homeobox protein SIX4PRO_0000049304Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserineBy similarity
Modified residuei634 – 6341PhosphoserineBy similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiQ61321.
PRIDEiQ61321.

PTM databases

PhosphoSiteiQ61321.

Expressioni

Tissue specificityi

Mainly expressed in the skeletal muscle (isoform 1 and isoform 2 but not isoform 3), and weakly in the heart. Also found in the retina and the distal tube of kidney.

Gene expression databases

BgeeiQ61321.
ExpressionAtlasiQ61321. baseline and differential.
GenevestigatoriQ61321.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
Kdm6aO705462EBI-986524,EBI-1573712

Protein-protein interaction databases

IntActiQ61321. 2 interactions.
MINTiMINT-7949316.

Structurei

3D structure databases

ProteinModelPortaliQ61321.
SMRiQ61321. Positions 97-272.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni582 – 775194Transactivation domainAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi2 – 54Poly-Ser
Compositional biasi58 – 614Poly-Ala
Compositional biasi70 – 767Poly-Ala
Compositional biasi92 – 954Poly-Ala

Sequence similaritiesi

Belongs to the SIX/Sine oculis homeobox family.Curated
Contains 1 homeobox DNA-binding domain.PROSITE-ProRule annotation

Keywords - Domaini

Homeobox

Phylogenomic databases

eggNOGiNOG244874.
GeneTreeiENSGT00540000070251.
HOGENOMiHOG000261651.
HOVERGENiHBG017802.
InParanoidiQ61321.
KOiK15615.
OMAiIKQENGM.
OrthoDBiEOG7C5M8Z.
PhylomeDBiQ61321.
TreeFamiTF315545.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
InterProiIPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR009057. Homeodomain-like.
[Graphical view]
PfamiPF00046. Homeobox. 1 hit.
[Graphical view]
SMARTiSM00389. HOX. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
PROSITEiPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. Align

Note: Additional isoforms seem to exist.

Isoform 1 (identifier: Q61321-1) [UniParc]FASTAAdd to Basket

Also known as: SM

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSSSSPTGQI ASAADIKQEN GMESASEGQE AHREVAGGAA AGLSPPAPAP
60 70 80 90 100
FPLEPGDAAA ASRVSREEGA AAAGAADQVQ LHSELLGRHQ HAAAAQPPLA
110 120 130 140 150
FSPDHVACVC EALQQGGNLD RLARFLWSLP QSDLLRGNES LLKARALVAF
160 170 180 190 200
HQGIYPELYS ILESHSFESA NHPLLQQLWY KARYTEAERA RGRPLGAVDK
210 220 230 240 250
YRLRRKFPLP RTIWDGEETV YCFKEKSRNA LKELYKQNRY PSPAEKRHLA
260 270 280 290 300
KITGLSLTQV SNWFKNRRQR DRNPSETQSK SESDGNPSTE DESSKGHEDL
310 320 330 340 350
SPHPLSGASD GVTNLSLSSH VEPVYMQQIG NAKISLSSSG VLLNGSLVPA
360 370 380 390 400
STSPVFLNGN SFIQGHNGVI LNGLNVGNTQ TVSLNPPKMS SNIVGNGIAM
410 420 430 440 450
TDILGSTSQD VKEFKVLQSS AVNSAATTSY SPSAPVSFPG LIPCTEVKRE
460 470 480 490 500
GIQTVASQDG GSVVTFTTPV QINQYGIVQI PNSGANGQFL NGSIGFSPLQ
510 520 530 540 550
LPPVSVAASQ GNLSVTPSTS DGSTFTSEPA TVQHGKLFLS PLTPSAVVYT
560 570 580 590 600
VPNSGQTVGA VKQEGLERGL VFSQLMPVNH SAQVNASLSS ENLSGSGLHP
610 620 630 640 650
LTSSLVNVSA AHGFSLTPPT LLNPTELNPD LAESQPVSAP VASKCTVSSV
660 670 680 690 700
SNTNYATLQN CSLIPGQDLL SGPMTQAALG EIVPTAEEQV SHASTAVHQD
710 720 730 740 750
FVREQRLVLQ SVPNIKENFL QNSENKATNN LMMLDSKSKY VLDGMVEAGC
760 770
EDLGTDKKEL AKLQTVQLDE DMQDL
Length:775
Mass (Da):82,263
Last modified:November 1, 1996 - v1
Checksum:iB06EBB64E04E5061
GO
Isoform 2 (identifier: Q61321-2) [UniParc]FASTAAdd to Basket

Also known as: M18

The sequence of this isoform differs from the canonical sequence as follows:
     2-9: SSSSPTGQ → QKAAIRLHYFALAAILM
     37-100: Missing.

Note: Incomplete sequence.

Show »
Length:720
Mass (Da):77,334
Checksum:iF893580411E05927
GO
Isoform 3 (identifier: Q61321-3) [UniParc]FASTAAdd to Basket

Also known as: M8

The sequence of this isoform differs from the canonical sequence as follows:
     2-9: SSSSPTGQ → QKAAIRLHYFALAAILM
     188-319: ERARGRPLGA...DGVTNLSLSS → AGNSPCPAPS...CNKLEMLRYH
     320-775: Missing.

Note: Incomplete sequence.

Show »
Length:328
Mass (Da):35,344
Checksum:iEE7E9E97952C03EB
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei2 – 98SSSSPTGQ → QKAAIRLHYFALAAILM in isoform 2 and isoform 3. CuratedVSP_002293
Alternative sequencei37 – 10064Missing in isoform 2. CuratedVSP_002294Add
BLAST
Alternative sequencei188 – 319132ERARG…LSLSS → AGNSPCPAPSGTARRRCIVS RRSRATRSRSSTSRIATPRR LRSGTWPRSPASPSPRSATG SRTGGSVTETPPRPSPKANR MATPVPRMNPARDMRICLLI HFQAHLMASPTSASLATWSQ YICNKLEMLRYH in isoform 3. CuratedVSP_002295Add
BLAST
Alternative sequencei320 – 775456Missing in isoform 3. CuratedVSP_002296Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D50416 mRNA. Translation: BAA08915.1.
D50417 mRNA. Translation: BAA08916.1.
D50418 mRNA. Translation: BAA08917.1.
BC137931 mRNA. Translation: AAI37932.1.
BC137934 mRNA. Translation: AAI37935.1.
CCDSiCCDS25974.1. [Q61321-1]
PIRiS63626.
S63628.
S63629.
RefSeqiNP_035512.1. NM_011382.2. [Q61321-1]
UniGeneiMm.249575.

Genome annotation databases

EnsembliENSMUST00000043208; ENSMUSP00000036150; ENSMUSG00000034460. [Q61321-1]
GeneIDi20474.
KEGGimmu:20474.
UCSCiuc007nwb.1. mouse. [Q61321-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D50416 mRNA. Translation: BAA08915.1 .
D50417 mRNA. Translation: BAA08916.1 .
D50418 mRNA. Translation: BAA08917.1 .
BC137931 mRNA. Translation: AAI37932.1 .
BC137934 mRNA. Translation: AAI37935.1 .
CCDSi CCDS25974.1. [Q61321-1 ]
PIRi S63626.
S63628.
S63629.
RefSeqi NP_035512.1. NM_011382.2. [Q61321-1 ]
UniGenei Mm.249575.

3D structure databases

ProteinModelPortali Q61321.
SMRi Q61321. Positions 97-272.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi Q61321. 2 interactions.
MINTi MINT-7949316.

PTM databases

PhosphoSitei Q61321.

Proteomic databases

MaxQBi Q61321.
PRIDEi Q61321.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000043208 ; ENSMUSP00000036150 ; ENSMUSG00000034460 . [Q61321-1 ]
GeneIDi 20474.
KEGGi mmu:20474.
UCSCi uc007nwb.1. mouse. [Q61321-1 ]

Organism-specific databases

CTDi 51804.
MGIi MGI:106034. Six4.

Phylogenomic databases

eggNOGi NOG244874.
GeneTreei ENSGT00540000070251.
HOGENOMi HOG000261651.
HOVERGENi HBG017802.
InParanoidi Q61321.
KOi K15615.
OMAi IKQENGM.
OrthoDBi EOG7C5M8Z.
PhylomeDBi Q61321.
TreeFami TF315545.

Miscellaneous databases

NextBioi 298591.
PROi Q61321.
SOURCEi Search...

Gene expression databases

Bgeei Q61321.
ExpressionAtlasi Q61321. baseline and differential.
Genevestigatori Q61321.

Family and domain databases

Gene3Di 1.10.10.60. 1 hit.
InterProi IPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR009057. Homeodomain-like.
[Graphical view ]
Pfami PF00046. Homeobox. 1 hit.
[Graphical view ]
SMARTi SM00389. HOX. 1 hit.
[Graphical view ]
SUPFAMi SSF46689. SSF46689. 1 hit.
PROSITEi PS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Structure, function and expression of a murine homeobox protein AREC3, a homologue of Drosophila sine oculis gene product, and implication in development."
    Kawakami K., Ohto H., Ikeda K., Roeder R.G.
    Nucleic Acids Res. 24:303-310(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), PARTIAL NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 2 AND 3).
    Strain: BALB/c.
    Tissue: Myoblast and Skeletal muscle.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Brain.

Entry informationi

Entry nameiSIX4_MOUSE
AccessioniPrimary (citable) accession number: Q61321
Secondary accession number(s): B2RQH3, Q61322, Q61323
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: October 29, 2014
This is version 133 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3