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Q61316 (HSP74_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 104. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Heat shock 70 kDa protein 4
Alternative name(s):
Heat shock 70-related protein APG-2
Gene names
Name:Hspa4
Synonyms:Apg2, Hsp110
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length841 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Subunit structure

Interacts with TJP1/ZO-1 By similarity.

Subcellular location

Cytoplasm Probable.

Sequence similarities

Belongs to the heat shock protein 70 family.

Ontologies

Keywords
   Biological processStress response
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   PTMAcetylation
Phosphoprotein
   Technical termComplete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Biological_processresponse to stress

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

protein binding

Inferred from physical interaction PubMed 23055941. Source: MGI

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 841841Heat shock 70 kDa protein 4
PRO_0000078263

Amino acid modifications

Modified residue531N6-acetyllysine Ref.6
Modified residue761Phosphoserine By similarity
Modified residue891Phosphotyrosine By similarity
Modified residue3361Phosphotyrosine Ref.3 Ref.5
Modified residue4301N6-acetyllysine By similarity
Modified residue5401Phosphothreonine By similarity
Modified residue5481Phosphoserine By similarity
Modified residue6611Phosphotyrosine Ref.3
Modified residue6801N6-acetyllysine By similarity

Sequences

Sequence LengthMass (Da)Tools
Q61316 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: E7FF19F555C115ED

FASTA84194,133
        10         20         30         40         50         60 
MSVVGIDLGF QSCYVAVARA GGIETIANEY SDRCTPACVS FGPKNRSIGA AAKSQVISNA 

        70         80         90        100        110        120 
KNTVQGFKRF HGRAFSDPFV EAEKSNLAYD IVQLPTGLTG IKVTYMEEER NFTTEQVTAM 

       130        140        150        160        170        180 
LLSKLKETAE SVLKKPVVDC VVSVPSFYTD AERRSVMDAT QIAGLNCLRL MNETTAVALA 

       190        200        210        220        230        240 
YGIYKQDLPA LEEKPRNVVF VDMGHSAYQV SVCAFNKGKL KVLATAFDTT LGGRKFDEVL 

       250        260        270        280        290        300 
VNHFCEEFGK KYKLDIKSKI RALLRLSQEC EKLKKLMSAN ASDLPLSIEC FMNDIDVSGT 

       310        320        330        340        350        360 
MNRGKFLEMC DDLLARVEPP LRSVLEQSKL KKEDIYAVEI VGGATRIPAV KEKISKFFGK 

       370        380        390        400        410        420 
ELSTTLNADE AVTRGCALQC AILSPAFKVR EFSITDVVPY PISLRWNSPA EEGLSDCEVF 

       430        440        450        460        470        480 
PKNHAAPFSK VLTFYRKEPF TLEAYYSSPQ DLPYPDPAIA QFSVQKVTPQ SDGSSSKVKV 

       490        500        510        520        530        540 
KVRVNVHGIF SVSSAALVEV HKSEESEEPM ETDQNAKEEE KMQVDQEEPH TEEQQQQPQT 

       550        560        570        580        590        600 
PAENKAESEE METSQAGSKD KKTDQPPQAK KAKVKTSTVD LPIEHTLWQL DREMLALYTE 

       610        620        630        640        650        660 
NEGKMIMQDK LEKERNDAKN AVEEYVYEMR DKLSGEYEKF VSEDDRNTFT LKLEDTENWL 

       670        680        690        700        710        720 
YEDGEDQPKQ VYVDKLAELK SLGQPIKTRF QESEERPKLF EELGKQIQQY MKVISSFKNK 

       730        740        750        760        770        780 
EDQYEHLDAA DVTKVEKSTN EAMEWMNSKL NLQNKQSLTV DPVVKTKEIE AKIKELTSIC 

       790        800        810        820        830        840 
SPIISKPKPK VEPPKEEPKH AEQNGPVDGQ GDNPGSQAAE HGADTAVPSD GDKKLPEMDI 


D 

« Hide

References

« Hide 'large scale' references
[1]"Cloning of apg-2 encoding a novel member of heat shock protein 110 family."
Kaneko Y., Kimura T., Kishishita M., Noda Y., Fujita J.
Gene 189:19-24(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: DDY/STD.
Tissue: Testis.
[2]Lubec G., Kang S.U., Sunyer B., Chen W.-Q.
Submitted (JAN-2009) to UniProtKB
Cited for: PROTEIN SEQUENCE OF 74-124; 155-169; 186-196; 220-234; 330-346; 361-374; 391-405; 699-705 AND 719-734, IDENTIFICATION BY MASS SPECTROMETRY.
Strain: C57BL/6 and OF1.
Tissue: Brain and Hippocampus.
[3]"Quantitative time-resolved phosphoproteomic analysis of mast cell signaling."
Cao L., Yu K., Banh C., Nguyen V., Ritz A., Raphael B.J., Kawakami Y., Kawakami T., Salomon A.R.
J. Immunol. 179:5864-5876(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-336 AND TYR-661, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Mast cell.
[4]"Large-scale phosphorylation analysis of mouse liver."
Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Liver.
[5]"Large-scale identification and evolution indexing of tyrosine phosphorylation sites from murine brain."
Ballif B.A., Carey G.R., Sunyaev S.R., Gygi S.P.
J. Proteome Res. 7:311-318(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-336, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Brain.
[6]"SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways."
Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.
Mol. Cell 50:919-930(2013) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-53, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Embryonic fibroblast.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D85904 mRNA. Translation: BAA12914.1.
UniGeneMm.239865.

3D structure databases

ProteinModelPortalQ61316.
SMRQ61316. Positions 5-698.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

DIPDIP-46965N.
IntActQ61316. 3 interactions.
MINTMINT-1869188.
STRING10090.ENSMUSP00000020630.

PTM databases

PhosphoSiteQ61316.

2D gel databases

REPRODUCTION-2DPAGEQ61316.

Proteomic databases

MaxQBQ61316.
PaxDbQ61316.
PRIDEQ61316.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Organism-specific databases

MGIMGI:1342292. Hspa4.

Phylogenomic databases

eggNOGCOG0443.
HOVERGENHBG047955.
InParanoidQ61316.

Gene expression databases

CleanExMM_HSPA4.
GenevestigatorQ61316.

Family and domain databases

Gene3D1.20.1270.10. 2 hits.
2.60.34.10. 2 hits.
InterProIPR018181. Heat_shock_70_CS.
IPR029048. HSP70_C.
IPR029047. HSP70_peptide-bd.
IPR013126. Hsp_70_fam.
[Graphical view]
PfamPF00012. HSP70. 2 hits.
[Graphical view]
PRINTSPR00301. HEATSHOCK70.
SUPFAMSSF100920. SSF100920. 1 hit.
SSF100934. SSF100934. 2 hits.
PROSITEPS00329. HSP70_2. 1 hit.
PS01036. HSP70_3. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSHSPA4. mouse.
PROQ61316.
SOURCESearch...

Entry information

Entry nameHSP74_MOUSE
AccessionPrimary (citable) accession number: Q61316
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: July 9, 2014
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot