Reviewed, UniProtKB/Swiss-Prot Q61312 (AP2C_MOUSE)

Names and origin

Protein names

Recommended name:
    Transcription factor AP-2 gamma
      Short name=AP2-gamma
Alternative name(s):
    Activating enhancer-binding protein 2 gamma
    AP-2.2

Gene names

Name:	Tfap2c
Synonyms:	Tcfap2c

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	449 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Sequence-specific DNA-binding protein that interacts with inducible viral and cellular enhancer elements to regulate transcription of selected genes. AP-2 factors bind to the consensus sequence 5'-GCCNNNGGC-3' and activate genes involved in a large spectrum of important biological functions including proper eye, face, body wall, limb and neural tube development. They also suppress a number of genes including MCAM/MUC18, C/EBP alpha and MYC.
Subunit structure	Binds DNA as a dimer. Can form homodimers or heterodimers with other AP-2 family members. Interacts with WWOX. Interacts with UBE2I By similarity. Interacts with CITED4.
Subcellular location	Nucleus Probable.
Tissue specificity	Expressed in lung, ovary and testis. Expressed in most squamous epithelia. Also, detected in several exocrine glands including the prostate, the preputial and salivary glands, serous glands of the tongue and ocular harderian glands.
Induction	During retinoic acid-mediated differentiation.
Domain	The WW-binding motif mediates interaction with WWOX By similarity.
Post-translational modification	Sumoylated on Lys-10; which inhibits transcriptional activity By similarity.
Sequence similarities	Belongs to the AP-2 family.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Ligand	DNA-binding
Molecular function	Activator
PTM	Isopeptide bond Phosphoprotein Ubl conjugation
Gene Ontology (GO)
Biological process	cell differentiation Inferred from mutant phenotype. Source: MGI regulation of transcription from RNA polymerase II promoter Inferred from direct assay. Source: MGI transcription Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	nucleus Inferred from direct assay. Source: MGI
Molecular function	protein binding Inferred from physical interaction. Source: MGI transcription factor activity Inferred from direct assay. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 449	449	Transcription factor AP-2 gamma		PRO_0000184804
Regions
	Region	292 – 423	132	H-S-H (helix-span-helix), dimerization
	Motif	59 – 64	6	WW-binding
	Compositional bias	30 – 109	80	Gln/Pro-rich (transactivation domain)
Amino acid modifications
	Modified residue	251	1	Phosphoserine; by PKA By similarity
	Modified residue	433	1	Phosphoserine By similarity
	Cross-link	10		Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q61312-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: DA5CAC4316EDF933	FASTA	449	49,151
	10 20 30 40 50 60 MLWKITDNVK YEEDCEDRHD SSSNGNPRIP HLSSPGQHLY SPAPPLSHTG VAEYQPPPYF 70 80 90 100 110 120 PPPYQQVVYS QSADHYSHLG EAYAAAMNPL HQPAATGSQQ QAWPGRQSQE GSSLASHHSR 130 140 150 160 170 180 SASLIPHISG LEGGSVSARR EVYRRSDLLL PHAHALEAGL AENLGLHEMA HPIEEVQNVD 190 200 210 220 230 240 DAHLLLHDQT VIRKGPISMT KNPLGLPCQK DLVGVVMNPS EVFCSVPGRL SLLSSTSKYK 250 260 270 280 290 300 VTVAEVQRRL SPPECLNASL LGGVLRRAKS KNGGRSLREK LDKIGLNLPA GRRKAAHVTL 310 320 330 340 350 360 LTSLVEGEAV HLARDFAYVC EAEFPSKAVA DYLTRPHLGG RNEMATRKSM LLAAQQVCKE 370 380 390 400 410 420 FTDLLHQDRT PNGNNRPAQV LEPNIQNCLS HFSLITHGFG SQAICAAVSA VQNYIKEALI 430 440 AIDKSYMNPG DQSPADSSKT MEKMEKHRK

References

[1]	"AP-2.2: a novel AP-2-related transcription factor induced by retinoic acid during differentiation of P19 embryonal carcinoma cells." Oulad-Abdelghani M., Bouillet P., Chazaud C., Dolle P., Chambon P. Exp. Cell Res. 225:338-347(1996) [PubMed: 8660922] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Embryonic carcinoma.
[2]	"Cloning of mouse Cited4, a member of the CITED family p300/CBP-binding transcriptional coactivators: induced expression in mammary epithelial cells." Yahata T., Takedatsu H., Dunwoodie S.L., Braganca J., Swingler T., Withington S.L., Hur J., Coser K.R., Isselbacher K.J., Bhattacharya S., Shioda T. Genomics 80:601-613(2002) [PubMed: 12504852] [Abstract] Cited for: INTERACTION WITH CITED4.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X94694 mRNA. Translation: CAA64357.1.
IPI	IPI00119908.
RefSeq	NP_033361.1.
UniGene	Mm.3629
3D structure databases
ModBase	Search...
PTM databases
PhosphoSite	Q61312.
Genome annotation databases
Ensembl	ENSMUSG00000028640. Mus musculus. [Contig view]
GeneID	21420.
KEGG	mmu:21420.
Organism-specific databases
MGI	MGI:106032. Tcfap2c.
Phylogenomic databases
HOVERGEN	Q61312.
Gene expression databases
ArrayExpress	Q61312.
Bgee	Q61312.
CleanEx	MM_TCFAP2C.
GermOnline	ENSMUSG00000028640. Mus musculus.
Family and domain databases
InterPro	IPR004979. TF_AP2. IPR013854. TF_AP2_C. IPR008123. TF_AP2_gamma. [Graphical view]
PANTHER	PTHR10812. TF_AP2. 1 hit.
Pfam	PF03299. TF_AP-2. 1 hit. [Graphical view]
PRINTS	PR01751. AP2CTNSCPFCT. PR01748. AP2TNSCPFCT.
ProtoNet	Search...
Other Resources
NextBio	300734.
SOURCE	Search...

Entry information

Entry name

AP2C_MOUSE

Accession

Primary (citable) accession number: Q61312

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 24, 2001
Last sequence update:	November 1, 1996
Last modified:	June 16, 2009
This is version 75 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)