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Q61290

- CAC1E_MOUSE

UniProt

Q61290 - CAC1E_MOUSE

Protein

Voltage-dependent R-type calcium channel subunit alpha-1E

Gene

Cacna1e

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 130 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Voltage-sensitive calcium channels (VSCC) mediate the entry of calcium ions into excitable cells and are also involved in a variety of calcium-dependent processes, including muscle contraction, hormone or neurotransmitter release, gene expression, cell motility, cell division and cell death. The isoform alpha-1E gives rise to R-type calcium currents. R-type calcium channels belong to the 'high-voltage activated' (HVA) group and are blocked by nickel, and partially by omega-agatoxin-IIIA (omega-Aga-IIIA). They are however insensitive to dihydropyridines (DHP), omega-conotoxin-GVIA (omega-CTx-GVIA), and omega-agatoxin-IVA (omega-Aga-IVA). Calcium channels containing alpha-1E subunit could be involved in the modulation of firing patterns of neurons which is important for information processing.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei310 – 3101Calcium ion selectivity and permeabilityBy similarity
    Sitei658 – 6581Calcium ion selectivity and permeabilityBy similarity
    Sitei1375 – 13751Calcium ion selectivity and permeabilityBy similarity
    Sitei1666 – 16661Calcium ion selectivity and permeabilityBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Calcium bindingi427 – 43812PROSITE-ProRule annotationAdd
    BLAST
    Calcium bindingi1755 – 176612PROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. calcium ion binding Source: InterPro
    2. high voltage-gated calcium channel activity Source: RefGenome
    3. protein N-terminus binding Source: UniProtKB
    4. voltage-gated calcium channel activity Source: MGI

    GO - Biological processi

    1. behavioral fear response Source: MGI
    2. behavioral response to pain Source: MGI
    3. calcium ion import Source: RefGenome
    4. calcium ion transmembrane transport Source: MGI
    5. calcium ion transport Source: MGI
    6. fear response Source: MGI
    7. glucose homeostasis Source: MGI
    8. locomotory behavior Source: MGI
    9. membrane depolarization during action potential Source: RefGenome
    10. neurological system process Source: MGI
    11. regulation of heart rate Source: MGI
    12. regulation of insulin secretion involved in cellular response to glucose stimulus Source: MGI
    13. regulation of somatostatin secretion Source: MGI
    14. response to pain Source: MGI
    15. sensory perception of pain Source: MGI
    16. sperm motility Source: MGI
    17. synaptic transmission Source: RefGenome
    18. transmission of nerve impulse Source: MGI
    19. visual learning Source: MGI

    Keywords - Molecular functioni

    Calcium channel, Ion channel, Voltage-gated channel

    Keywords - Biological processi

    Calcium transport, Ion transport, Transport

    Keywords - Ligandi

    Calcium, Metal-binding

    Protein family/group databases

    TCDBi1.A.1.11.3. the voltage-gated ion channel (vic) superfamily.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Voltage-dependent R-type calcium channel subunit alpha-1E
    Alternative name(s):
    Brain calcium channel II
    Short name:
    BII
    Calcium channel, L type, alpha-1 polypeptide, isoform 6
    Voltage-gated calcium channel subunit alpha Cav2.3
    Gene namesi
    Name:Cacna1e
    Synonyms:Cach6, Cacnl1a6, Cchra1
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Unplaced

    Organism-specific databases

    MGIiMGI:106217. Cacna1e.

    Subcellular locationi

    GO - Cellular componenti

    1. plasma membrane Source: RefGenome
    2. voltage-gated calcium channel complex Source: MGI

    Keywords - Cellular componenti

    Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 22722272Voltage-dependent R-type calcium channel subunit alpha-1EPRO_0000053939Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi255 – 2551N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi1569 – 15691N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi1692 – 16921N-linked (GlcNAc...)Sequence Analysis
    Modified residuei1737 – 17371Phosphoserine; by PKASequence Analysis

    Keywords - PTMi

    Disulfide bond, Glycoprotein, Phosphoprotein

    Proteomic databases

    PaxDbiQ61290.
    PRIDEiQ61290.

    PTM databases

    PhosphoSiteiQ61290.

    Expressioni

    Tissue specificityi

    Expressed in neuronal tissues, retina, spleen, and pancreatic islet cells.

    Gene expression databases

    GenevestigatoriQ61290.

    Interactioni

    Subunit structurei

    Interacts with EFHC1. Voltage-dependent calcium channels are multisubunit complexes, consisting of alpha-1, alpha-2, beta and delta subunits in a 1:1:1:1 ratio. The channel activity is directed by the pore-forming and voltage-sensitive alpha-1 subunit. In many cases, this subunit is sufficient to generate voltage-sensitive calcium channel activity. The auxiliary subunits beta and alpha-2/delta linked by a disulfide bridge regulate the channel activity.

    Protein-protein interaction databases

    IntActiQ61290. 2 interactions.

    Structurei

    3D structure databases

    ProteinModelPortaliQ61290.
    SMRiQ61290. Positions 359-403, 1252-1279.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 9090CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini110 – 12819ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini148 – 15912CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini175 – 18612ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini207 – 22418CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini246 – 32782ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini352 – 477126CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini498 – 51013ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini531 – 5399CytoplasmicSequence Analysis
    Topological domaini559 – 56810ExtracellularSequence Analysis
    Topological domaini588 – 60619CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini627 – 67953ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini705 – 1150446CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini1168 – 119124ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini1212 – 12198CytoplasmicSequence Analysis
    Topological domaini1243 – 125614ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini1275 – 129319CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini1314 – 140087ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini1425 – 148157CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini1501 – 151515ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini1536 – 15438CytoplasmicSequence Analysis
    Topological domaini1563 – 157311ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini1593 – 161119CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini1632 – 170069ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini1727 – 2272546CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei91 – 10919Helical; Name=S1 of repeat ISequence AnalysisAdd
    BLAST
    Transmembranei129 – 14719Helical; Name=S2 of repeat ISequence AnalysisAdd
    BLAST
    Transmembranei160 – 17415Helical; Name=S3 of repeat ISequence AnalysisAdd
    BLAST
    Transmembranei187 – 20620Helical; Name=S4 of repeat ISequence AnalysisAdd
    BLAST
    Transmembranei225 – 24521Helical; Name=S5 of repeat ISequence AnalysisAdd
    BLAST
    Transmembranei328 – 35124Helical; Name=S6 of repeat ISequence AnalysisAdd
    BLAST
    Transmembranei478 – 49720Helical; Name=S1 of repeat IISequence AnalysisAdd
    BLAST
    Transmembranei511 – 53020Helical; Name=S2 of repeat IISequence AnalysisAdd
    BLAST
    Transmembranei540 – 55819Helical; Name=S3 of repeat IISequence AnalysisAdd
    BLAST
    Transmembranei569 – 58719Helical; Name=S4 of repeat IISequence AnalysisAdd
    BLAST
    Transmembranei607 – 62620Helical; Name=S5 of repeat IISequence AnalysisAdd
    BLAST
    Transmembranei680 – 70425Helical; Name=S6 of repeat IISequence AnalysisAdd
    BLAST
    Transmembranei1151 – 116717Helical; Name=S1 of repeat IIISequence AnalysisAdd
    BLAST
    Transmembranei1192 – 121120Helical; Name=S2 of repeat IIISequence AnalysisAdd
    BLAST
    Transmembranei1220 – 124223Helical; Name=S3 of repeat IIISequence AnalysisAdd
    BLAST
    Transmembranei1257 – 127418Helical; Name=S4 of repeat IIISequence AnalysisAdd
    BLAST
    Transmembranei1294 – 131320Helical; Name=S5 of repeat IIISequence AnalysisAdd
    BLAST
    Transmembranei1401 – 142424Helical; Name=S6 of repeat IIISequence AnalysisAdd
    BLAST
    Transmembranei1482 – 150019Helical; Name=S1 of repeat IVSequence AnalysisAdd
    BLAST
    Transmembranei1516 – 153520Helical; Name=S2 of repeat IVSequence AnalysisAdd
    BLAST
    Transmembranei1544 – 156219Helical; Name=S3 of repeat IVSequence AnalysisAdd
    BLAST
    Transmembranei1574 – 159219Helical; Name=S4 of repeat IVSequence AnalysisAdd
    BLAST
    Transmembranei1612 – 163120Helical; Name=S5 of repeat IVSequence AnalysisAdd
    BLAST
    Transmembranei1701 – 172626Helical; Name=S6 of repeat IVSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati77 – 355279IAdd
    BLAST
    Repeati463 – 707245IIAdd
    BLAST
    Repeati1143 – 1429287IIIAdd
    BLAST
    Repeati1466 – 1729264IVAdd
    BLAST
    Domaini1742 – 177736EF-handPROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni375 – 39218Binding to the beta subunitBy similarityAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi717 – 7226Poly-Glu
    Compositional biasi751 – 7544Poly-Arg
    Compositional biasi770 – 7734Poly-Arg
    Compositional biasi1108 – 11125Poly-Glu
    Compositional biasi1115 – 11184Poly-Lys
    Compositional biasi1231 – 12344Poly-Val
    Compositional biasi2244 – 22474Poly-Arg

    Domaini

    Each of the four internal repeats contains five hydrophobic transmembrane segments (S1, S2, S3, S5, S6) and one positively charged transmembrane segment (S4). S4 segments probably represent the voltage-sensor and are characterized by a series of positively charged amino acids at every third position.

    Sequence similaritiesi

    Contains 1 EF-hand domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG1226.
    HOVERGENiHBG050763.
    InParanoidiQ61290.
    PhylomeDBiQ61290.

    Family and domain databases

    Gene3Di1.20.120.350. 4 hits.
    InterProiIPR027359. Channel_four-helix_dom.
    IPR002048. EF_hand_dom.
    IPR005821. Ion_trans_dom.
    IPR014873. VDCC_a1su_IQ.
    IPR005449. VDCC_R_a1su.
    IPR002077. VDCCAlpha1.
    [Graphical view]
    PANTHERiPTHR10037:SF57. PTHR10037:SF57. 1 hit.
    PfamiPF08763. Ca_chan_IQ. 1 hit.
    PF00520. Ion_trans. 4 hits.
    [Graphical view]
    PRINTSiPR00167. CACHANNEL.
    PR01633. RVDCCALPHA1.
    SMARTiSM01062. Ca_chan_IQ. 1 hit.
    [Graphical view]
    PROSITEiPS50222. EF_HAND_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q61290-1 [UniParc]FASTAAdd to Basket

    « Hide

    MARFGEAVVV GRPGSGDGDS DQSRNRQGTP VPASGPAAAY KQSKAQRART     50
    MALYNPIPVR QNCFTVNRSL FIFGEDNIVR KYAKKLIDWP PFEYMILATI 100
    IANCIVLALE QHLPEDDKTP MSRRLEKTEP YFIGIFCFEA GIKIVALGFI 150
    FHKGSYLRNG WNVMDFIVVL SGILATAGTH FNTHVDLRAL RAVRVLRPLK 200
    LVSGIPSLQI VLKSIMKAMV PLLQIGLLLF FAILMFAIIG LEFYSGKLHR 250
    ACFMNNSGIL EGFDPPHPCG VQGCPAGYEC KDWIGPNDGI TQFDNILFAV 300
    LTVFQCITME GWTTVLYNTN DALGATWNWL YFIPLIIIGS FFVLNLVLGV 350
    LSGEFAKERE RVENRRAFMK LRRQQQIERE LNGYRAWIDK AEEVMLAEEN 400
    KNSGTSALEV LRRATIKRSR TEAMTRDSSD EHCVDISSVG TPLARASIKS 450
    TKVDGASYFR HKERLLRISI RHMVKSQVFY WIVLSVVALN TACVAIVHHN 500
    QPQWLTHLLY YAEFLFLGLF LLEMSLKMYG MGPRLYFHSS FNCFDFGVTV 550
    GSIFEVVWAI FRPGTSFGIS VLRALRLLRI FKITKYWASL RNLVVSLMSS 600
    MKSIISLLFL LFLFIVVFAL LGMQLFGGRF NFNDGTPSAN FDTFPAAIMT 650
    VFQILTGEDW NEVMYNGIRS QGGVSSGMWS AIYFIVLTLF GNYTLLNVFL 700
    AIAVDNLANA QELTKDEQEE EEAFNQKHAL QKAKEVSPMS APNMPSIERD 750
    RRRRHHMSMW EPRSSHLRER RRRHHMSVWE QRTSQLRRHM QMSSQEALNK 800
    EEAPPMNPLN PLNPLSPLNP LNAHPSLYRR PRPIEGLALG LGLEKCEEER 850
    ISRGGSLKGD IGGLTSALDN QRSPLSLGKR EPPWLPRSCH GNCDPIQQEA 900
    GGGETVVTFE DRARHRQSQR RSRHRRVRTE GKDSASASRS RSASQERSLD 950
    EGVSVEGEKE HEPHSSHRSK EPTIHEEERT QDLRRTNSLM VPRGSGLVGA 1000
    LDEAETPLVQ PQPELEVGKD AALTEQEAEG SSEQALLGDV QLDVGRGISQ 1050
    SEPDLSCMTA NMDKATTEST SVTVAIPDVD PLVDSTVVNI SNKTDGEASP 1100
    LKEAETKEEE EEVEKKKKQK KEKRETGKAM VPHSSMFIFS TTNPIRRACH 1150
    YIVNLRYFEM CILLVIAASS IALAAEDPVL TNSERNKVLR YFDYVFTGVF 1200
    TFEMVIKMID QGLILQDGSY FRDLWNILDF VVVVGALVAF ALANALGTNK 1250
    GRDIKTIKSL RVLRVLRPLK TIKRLPKLKA VFDCVVTSLK NVFNILIVYK 1300
    LFMFIFAVIA VQLFKGKFFY CTDSSKDTEK ECIGNYVDHE KNKMEVKGRE 1350
    WKRHEFHYDN IIWALLTLFT VSTGEGWPQV LQHSVDVTEE DRGPSRSNRM 1400
    EMSIFYVVYF VVFPFFFVNI FVALIIITFQ EQGDKMMEEC SLEKNERACI 1450
    DFAISAKPLT RYMPQNRHTF QYRVWHFVVS PSFEYTIMAM IALNTVVLMM 1500
    KYYTAPCTYE LALKYLNIAF TMVFSLECVL KVIAFGFLNY FRDTWNIFDF 1550
    ITVIGSITEI ILTDSKLVNT SGFNMSFLKL FRAARLIKLL RQGYTIRILL 1600
    WTFVQSFKAL PYVCLLIAML FFIYAIIGMQ VFGNIKLDEE SHINRHNNFR 1650
    SFFGSLMLLF RSATGEAWQE IMLSCLGEKG CEPDTTAPSG QNESERCGTD 1700
    LAYVYFVSFI FFCSFLMLNL FVAVIMDNFE YLTRDSSILG PHHLDEFVRV 1750
    WAEYDRAACG RIHYTEMYEM LTLMSPPLGL GKRCPSKVAY KRLVLMNMPV 1800
    AEDMTVHFTS TLMALIRTAL DIKIAKGGAD RQQLDSELQK ETLAIWPHLS 1850
    QKMLDLLVPM PKASDLTVGK IYAAMMIMDY YKQSKVKKQR QQLEEQKNAP 1900
    MFQRMEPSSL PQEIIANAKA LPYLQQDPVS GLSGRSGYPS MSPLSPQEIF 1950
    QLACMDPADD GQFQEQQSLV VTDPSSMRRS FSTIRDKRSN SSWLEEFSME 2000
    RSSENTYKSR RRSYHSSLRL SAHRLNSDSG HKSDTHRSGG RERGRSKERK 2050
    HLLSPDVSRC NSEERGTQAD WESPERRQSR SPSEGRSQTP NRQGTGSLSE 2100
    SSIPSISDTS TPRRSRRQLP PVPPKPRPLL SYSSLMRHTG GISPPPDGSE 2150
    GGSPLASQAL ESNSACLTES SNSLHPQQGQ HPSPQHYISE PYLALHEDSH 2200
    ASDCGEEETL TFEAAVATSL GRSNTIGSAP PLRHSWQMPN GHYRRRRWGA 2250
    WAGMMCGAVS DLLSDTEEDD KC 2272
    Length:2,272
    Mass (Da):257,236
    Last modified:November 1, 1996 - v1
    Checksum:i70D9200B9E0C87A1
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L29346 mRNA. Translation: AAA59206.1.
    PIRiC54972.
    UniGeneiMm.267517.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L29346 mRNA. Translation: AAA59206.1 .
    PIRi C54972.
    UniGenei Mm.267517.

    3D structure databases

    ProteinModelPortali Q61290.
    SMRi Q61290. Positions 359-403, 1252-1279.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q61290. 2 interactions.

    Protein family/group databases

    TCDBi 1.A.1.11.3. the voltage-gated ion channel (vic) superfamily.

    PTM databases

    PhosphoSitei Q61290.

    Proteomic databases

    PaxDbi Q61290.
    PRIDEi Q61290.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Organism-specific databases

    MGIi MGI:106217. Cacna1e.

    Phylogenomic databases

    eggNOGi COG1226.
    HOVERGENi HBG050763.
    InParanoidi Q61290.
    PhylomeDBi Q61290.

    Miscellaneous databases

    PROi Q61290.
    SOURCEi Search...

    Gene expression databases

    Genevestigatori Q61290.

    Family and domain databases

    Gene3Di 1.20.120.350. 4 hits.
    InterProi IPR027359. Channel_four-helix_dom.
    IPR002048. EF_hand_dom.
    IPR005821. Ion_trans_dom.
    IPR014873. VDCC_a1su_IQ.
    IPR005449. VDCC_R_a1su.
    IPR002077. VDCCAlpha1.
    [Graphical view ]
    PANTHERi PTHR10037:SF57. PTHR10037:SF57. 1 hit.
    Pfami PF08763. Ca_chan_IQ. 1 hit.
    PF00520. Ion_trans. 4 hits.
    [Graphical view ]
    PRINTSi PR00167. CACHANNEL.
    PR01633. RVDCCALPHA1.
    SMARTi SM01062. Ca_chan_IQ. 1 hit.
    [Graphical view ]
    PROSITEi PS50222. EF_HAND_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Structure and functional characterization of neuronal alpha 1E calcium channel subtypes."
      Williams M.E., Marubio L.M., Deal C.R., Hans M., Brust P.F., Philipson L.H., Miller R.J., Johnson E.C., Harpold M.M., Ellis S.B.
      J. Biol. Chem. 269:22347-22357(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: BALB/c.
      Tissue: Brain.
    2. "Qualitative and quantitative analyses of protein phosphorylation in naive and stimulated mouse synaptosomal preparations."
      Munton R.P., Tweedie-Cullen R., Livingstone-Zatchej M., Weinandy F., Waidelich M., Longo D., Gehrig P., Potthast F., Rutishauser D., Gerrits B., Panse C., Schlapbach R., Mansuy I.M.
      Mol. Cell. Proteomics 6:283-293(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Brain cortex.

    Entry informationi

    Entry nameiCAC1E_MOUSE
    AccessioniPrimary (citable) accession number: Q61290
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 15, 1999
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 130 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3