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Q61290

- CAC1E_MOUSE

UniProt

Q61290 - CAC1E_MOUSE

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Protein

Voltage-dependent R-type calcium channel subunit alpha-1E

Gene
Cacna1e, Cach6, Cacnl1a6, Cchra1
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Voltage-sensitive calcium channels (VSCC) mediate the entry of calcium ions into excitable cells and are also involved in a variety of calcium-dependent processes, including muscle contraction, hormone or neurotransmitter release, gene expression, cell motility, cell division and cell death. The isoform alpha-1E gives rise to R-type calcium currents. R-type calcium channels belong to the 'high-voltage activated' (HVA) group and are blocked by nickel, and partially by omega-agatoxin-IIIA (omega-Aga-IIIA). They are however insensitive to dihydropyridines (DHP), omega-conotoxin-GVIA (omega-CTx-GVIA), and omega-agatoxin-IVA (omega-Aga-IVA). Calcium channels containing alpha-1E subunit could be involved in the modulation of firing patterns of neurons which is important for information processing.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei310 – 3101Calcium ion selectivity and permeability By similarity
Sitei658 – 6581Calcium ion selectivity and permeability By similarity
Sitei1375 – 13751Calcium ion selectivity and permeability By similarity
Sitei1666 – 16661Calcium ion selectivity and permeability By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Calcium bindingi427 – 43812 By similarityAdd
BLAST
Calcium bindingi1755 – 176612 By similarityAdd
BLAST

GO - Molecular functioni

  1. calcium ion binding Source: InterPro
  2. high voltage-gated calcium channel activity Source: RefGenome
  3. protein N-terminus binding Source: UniProtKB
  4. voltage-gated calcium channel activity Source: MGI

GO - Biological processi

  1. behavioral fear response Source: MGI
  2. behavioral response to pain Source: MGI
  3. calcium ion import Source: RefGenome
  4. calcium ion transmembrane transport Source: MGI
  5. calcium ion transport Source: MGI
  6. fear response Source: MGI
  7. glucose homeostasis Source: MGI
  8. locomotory behavior Source: MGI
  9. membrane depolarization during action potential Source: RefGenome
  10. neurological system process Source: MGI
  11. regulation of heart rate Source: MGI
  12. regulation of insulin secretion involved in cellular response to glucose stimulus Source: MGI
  13. regulation of somatostatin secretion Source: MGI
  14. response to pain Source: MGI
  15. sensory perception of pain Source: MGI
  16. sperm motility Source: MGI
  17. synaptic transmission Source: RefGenome
  18. transmission of nerve impulse Source: MGI
  19. visual learning Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Calcium channel, Ion channel, Voltage-gated channel

Keywords - Biological processi

Calcium transport, Ion transport, Transport

Keywords - Ligandi

Calcium, Metal-binding

Protein family/group databases

TCDBi1.A.1.11.3. the voltage-gated ion channel (vic) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
Voltage-dependent R-type calcium channel subunit alpha-1E
Alternative name(s):
Brain calcium channel II
Short name:
BII
Calcium channel, L type, alpha-1 polypeptide, isoform 6
Voltage-gated calcium channel subunit alpha Cav2.3
Gene namesi
Name:Cacna1e
Synonyms:Cach6, Cacnl1a6, Cchra1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Unplaced

Organism-specific databases

MGIiMGI:106217. Cacna1e.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 9090Cytoplasmic Reviewed predictionAdd
BLAST
Transmembranei91 – 10919Helical; Name=S1 of repeat I; Reviewed predictionAdd
BLAST
Topological domaini110 – 12819Extracellular Reviewed predictionAdd
BLAST
Transmembranei129 – 14719Helical; Name=S2 of repeat I; Reviewed predictionAdd
BLAST
Topological domaini148 – 15912Cytoplasmic Reviewed predictionAdd
BLAST
Transmembranei160 – 17415Helical; Name=S3 of repeat I; Reviewed predictionAdd
BLAST
Topological domaini175 – 18612Extracellular Reviewed predictionAdd
BLAST
Transmembranei187 – 20620Helical; Name=S4 of repeat I; Reviewed predictionAdd
BLAST
Topological domaini207 – 22418Cytoplasmic Reviewed predictionAdd
BLAST
Transmembranei225 – 24521Helical; Name=S5 of repeat I; Reviewed predictionAdd
BLAST
Topological domaini246 – 32782Extracellular Reviewed predictionAdd
BLAST
Transmembranei328 – 35124Helical; Name=S6 of repeat I; Reviewed predictionAdd
BLAST
Topological domaini352 – 477126Cytoplasmic Reviewed predictionAdd
BLAST
Transmembranei478 – 49720Helical; Name=S1 of repeat II; Reviewed predictionAdd
BLAST
Topological domaini498 – 51013Extracellular Reviewed predictionAdd
BLAST
Transmembranei511 – 53020Helical; Name=S2 of repeat II; Reviewed predictionAdd
BLAST
Topological domaini531 – 5399Cytoplasmic Reviewed prediction
Transmembranei540 – 55819Helical; Name=S3 of repeat II; Reviewed predictionAdd
BLAST
Topological domaini559 – 56810Extracellular Reviewed prediction
Transmembranei569 – 58719Helical; Name=S4 of repeat II; Reviewed predictionAdd
BLAST
Topological domaini588 – 60619Cytoplasmic Reviewed predictionAdd
BLAST
Transmembranei607 – 62620Helical; Name=S5 of repeat II; Reviewed predictionAdd
BLAST
Topological domaini627 – 67953Extracellular Reviewed predictionAdd
BLAST
Transmembranei680 – 70425Helical; Name=S6 of repeat II; Reviewed predictionAdd
BLAST
Topological domaini705 – 1150446Cytoplasmic Reviewed predictionAdd
BLAST
Transmembranei1151 – 116717Helical; Name=S1 of repeat III; Reviewed predictionAdd
BLAST
Topological domaini1168 – 119124Extracellular Reviewed predictionAdd
BLAST
Transmembranei1192 – 121120Helical; Name=S2 of repeat III; Reviewed predictionAdd
BLAST
Topological domaini1212 – 12198Cytoplasmic Reviewed prediction
Transmembranei1220 – 124223Helical; Name=S3 of repeat III; Reviewed predictionAdd
BLAST
Topological domaini1243 – 125614Extracellular Reviewed predictionAdd
BLAST
Transmembranei1257 – 127418Helical; Name=S4 of repeat III; Reviewed predictionAdd
BLAST
Topological domaini1275 – 129319Cytoplasmic Reviewed predictionAdd
BLAST
Transmembranei1294 – 131320Helical; Name=S5 of repeat III; Reviewed predictionAdd
BLAST
Topological domaini1314 – 140087Extracellular Reviewed predictionAdd
BLAST
Transmembranei1401 – 142424Helical; Name=S6 of repeat III; Reviewed predictionAdd
BLAST
Topological domaini1425 – 148157Cytoplasmic Reviewed predictionAdd
BLAST
Transmembranei1482 – 150019Helical; Name=S1 of repeat IV; Reviewed predictionAdd
BLAST
Topological domaini1501 – 151515Extracellular Reviewed predictionAdd
BLAST
Transmembranei1516 – 153520Helical; Name=S2 of repeat IV; Reviewed predictionAdd
BLAST
Topological domaini1536 – 15438Cytoplasmic Reviewed prediction
Transmembranei1544 – 156219Helical; Name=S3 of repeat IV; Reviewed predictionAdd
BLAST
Topological domaini1563 – 157311Extracellular Reviewed predictionAdd
BLAST
Transmembranei1574 – 159219Helical; Name=S4 of repeat IV; Reviewed predictionAdd
BLAST
Topological domaini1593 – 161119Cytoplasmic Reviewed predictionAdd
BLAST
Transmembranei1612 – 163120Helical; Name=S5 of repeat IV; Reviewed predictionAdd
BLAST
Topological domaini1632 – 170069Extracellular Reviewed predictionAdd
BLAST
Transmembranei1701 – 172626Helical; Name=S6 of repeat IV; Reviewed predictionAdd
BLAST
Topological domaini1727 – 2272546Cytoplasmic Reviewed predictionAdd
BLAST

GO - Cellular componenti

  1. plasma membrane Source: RefGenome
  2. voltage-gated calcium channel complex Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 22722272Voltage-dependent R-type calcium channel subunit alpha-1EPRO_0000053939Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi255 – 2551N-linked (GlcNAc...) Reviewed prediction
Glycosylationi1569 – 15691N-linked (GlcNAc...) Reviewed prediction
Glycosylationi1692 – 16921N-linked (GlcNAc...) Reviewed prediction
Modified residuei1737 – 17371Phosphoserine; by PKA Reviewed prediction

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiQ61290.
PRIDEiQ61290.

PTM databases

PhosphoSiteiQ61290.

Expressioni

Tissue specificityi

Expressed in neuronal tissues, retina, spleen, and pancreatic islet cells.

Gene expression databases

GenevestigatoriQ61290.

Interactioni

Subunit structurei

Interacts with EFHC1. Voltage-dependent calcium channels are multisubunit complexes, consisting of alpha-1, alpha-2, beta and delta subunits in a 1:1:1:1 ratio. The channel activity is directed by the pore-forming and voltage-sensitive alpha-1 subunit. In many cases, this subunit is sufficient to generate voltage-sensitive calcium channel activity. The auxiliary subunits beta and alpha-2/delta linked by a disulfide bridge regulate the channel activity.

Protein-protein interaction databases

IntActiQ61290. 2 interactions.

Structurei

3D structure databases

ProteinModelPortaliQ61290.
SMRiQ61290. Positions 359-403, 1252-1279.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati77 – 355279IAdd
BLAST
Repeati463 – 707245IIAdd
BLAST
Repeati1143 – 1429287IIIAdd
BLAST
Repeati1466 – 1729264IVAdd
BLAST
Domaini1742 – 177736EF-handAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni375 – 39218Binding to the beta subunit By similarityAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi717 – 7226Poly-Glu
Compositional biasi751 – 7544Poly-Arg
Compositional biasi770 – 7734Poly-Arg
Compositional biasi1108 – 11125Poly-Glu
Compositional biasi1115 – 11184Poly-Lys
Compositional biasi1231 – 12344Poly-Val
Compositional biasi2244 – 22474Poly-Arg

Domaini

Each of the four internal repeats contains five hydrophobic transmembrane segments (S1, S2, S3, S5, S6) and one positively charged transmembrane segment (S4). S4 segments probably represent the voltage-sensor and are characterized by a series of positively charged amino acids at every third position.

Sequence similaritiesi

Contains 1 EF-hand domain.

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG1226.
HOVERGENiHBG050763.
InParanoidiQ61290.
PhylomeDBiQ61290.

Family and domain databases

Gene3Di1.20.120.350. 4 hits.
InterProiIPR027359. Channel_four-helix_dom.
IPR002048. EF_hand_dom.
IPR005821. Ion_trans_dom.
IPR014873. VDCC_a1su_IQ.
IPR005449. VDCC_R_a1su.
IPR002077. VDCCAlpha1.
[Graphical view]
PANTHERiPTHR10037:SF57. PTHR10037:SF57. 1 hit.
PfamiPF08763. Ca_chan_IQ. 1 hit.
PF00520. Ion_trans. 4 hits.
[Graphical view]
PRINTSiPR00167. CACHANNEL.
PR01633. RVDCCALPHA1.
SMARTiSM01062. Ca_chan_IQ. 1 hit.
[Graphical view]
PROSITEiPS50222. EF_HAND_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q61290-1 [UniParc]FASTAAdd to Basket

« Hide

MARFGEAVVV GRPGSGDGDS DQSRNRQGTP VPASGPAAAY KQSKAQRART     50
MALYNPIPVR QNCFTVNRSL FIFGEDNIVR KYAKKLIDWP PFEYMILATI 100
IANCIVLALE QHLPEDDKTP MSRRLEKTEP YFIGIFCFEA GIKIVALGFI 150
FHKGSYLRNG WNVMDFIVVL SGILATAGTH FNTHVDLRAL RAVRVLRPLK 200
LVSGIPSLQI VLKSIMKAMV PLLQIGLLLF FAILMFAIIG LEFYSGKLHR 250
ACFMNNSGIL EGFDPPHPCG VQGCPAGYEC KDWIGPNDGI TQFDNILFAV 300
LTVFQCITME GWTTVLYNTN DALGATWNWL YFIPLIIIGS FFVLNLVLGV 350
LSGEFAKERE RVENRRAFMK LRRQQQIERE LNGYRAWIDK AEEVMLAEEN 400
KNSGTSALEV LRRATIKRSR TEAMTRDSSD EHCVDISSVG TPLARASIKS 450
TKVDGASYFR HKERLLRISI RHMVKSQVFY WIVLSVVALN TACVAIVHHN 500
QPQWLTHLLY YAEFLFLGLF LLEMSLKMYG MGPRLYFHSS FNCFDFGVTV 550
GSIFEVVWAI FRPGTSFGIS VLRALRLLRI FKITKYWASL RNLVVSLMSS 600
MKSIISLLFL LFLFIVVFAL LGMQLFGGRF NFNDGTPSAN FDTFPAAIMT 650
VFQILTGEDW NEVMYNGIRS QGGVSSGMWS AIYFIVLTLF GNYTLLNVFL 700
AIAVDNLANA QELTKDEQEE EEAFNQKHAL QKAKEVSPMS APNMPSIERD 750
RRRRHHMSMW EPRSSHLRER RRRHHMSVWE QRTSQLRRHM QMSSQEALNK 800
EEAPPMNPLN PLNPLSPLNP LNAHPSLYRR PRPIEGLALG LGLEKCEEER 850
ISRGGSLKGD IGGLTSALDN QRSPLSLGKR EPPWLPRSCH GNCDPIQQEA 900
GGGETVVTFE DRARHRQSQR RSRHRRVRTE GKDSASASRS RSASQERSLD 950
EGVSVEGEKE HEPHSSHRSK EPTIHEEERT QDLRRTNSLM VPRGSGLVGA 1000
LDEAETPLVQ PQPELEVGKD AALTEQEAEG SSEQALLGDV QLDVGRGISQ 1050
SEPDLSCMTA NMDKATTEST SVTVAIPDVD PLVDSTVVNI SNKTDGEASP 1100
LKEAETKEEE EEVEKKKKQK KEKRETGKAM VPHSSMFIFS TTNPIRRACH 1150
YIVNLRYFEM CILLVIAASS IALAAEDPVL TNSERNKVLR YFDYVFTGVF 1200
TFEMVIKMID QGLILQDGSY FRDLWNILDF VVVVGALVAF ALANALGTNK 1250
GRDIKTIKSL RVLRVLRPLK TIKRLPKLKA VFDCVVTSLK NVFNILIVYK 1300
LFMFIFAVIA VQLFKGKFFY CTDSSKDTEK ECIGNYVDHE KNKMEVKGRE 1350
WKRHEFHYDN IIWALLTLFT VSTGEGWPQV LQHSVDVTEE DRGPSRSNRM 1400
EMSIFYVVYF VVFPFFFVNI FVALIIITFQ EQGDKMMEEC SLEKNERACI 1450
DFAISAKPLT RYMPQNRHTF QYRVWHFVVS PSFEYTIMAM IALNTVVLMM 1500
KYYTAPCTYE LALKYLNIAF TMVFSLECVL KVIAFGFLNY FRDTWNIFDF 1550
ITVIGSITEI ILTDSKLVNT SGFNMSFLKL FRAARLIKLL RQGYTIRILL 1600
WTFVQSFKAL PYVCLLIAML FFIYAIIGMQ VFGNIKLDEE SHINRHNNFR 1650
SFFGSLMLLF RSATGEAWQE IMLSCLGEKG CEPDTTAPSG QNESERCGTD 1700
LAYVYFVSFI FFCSFLMLNL FVAVIMDNFE YLTRDSSILG PHHLDEFVRV 1750
WAEYDRAACG RIHYTEMYEM LTLMSPPLGL GKRCPSKVAY KRLVLMNMPV 1800
AEDMTVHFTS TLMALIRTAL DIKIAKGGAD RQQLDSELQK ETLAIWPHLS 1850
QKMLDLLVPM PKASDLTVGK IYAAMMIMDY YKQSKVKKQR QQLEEQKNAP 1900
MFQRMEPSSL PQEIIANAKA LPYLQQDPVS GLSGRSGYPS MSPLSPQEIF 1950
QLACMDPADD GQFQEQQSLV VTDPSSMRRS FSTIRDKRSN SSWLEEFSME 2000
RSSENTYKSR RRSYHSSLRL SAHRLNSDSG HKSDTHRSGG RERGRSKERK 2050
HLLSPDVSRC NSEERGTQAD WESPERRQSR SPSEGRSQTP NRQGTGSLSE 2100
SSIPSISDTS TPRRSRRQLP PVPPKPRPLL SYSSLMRHTG GISPPPDGSE 2150
GGSPLASQAL ESNSACLTES SNSLHPQQGQ HPSPQHYISE PYLALHEDSH 2200
ASDCGEEETL TFEAAVATSL GRSNTIGSAP PLRHSWQMPN GHYRRRRWGA 2250
WAGMMCGAVS DLLSDTEEDD KC 2272
Length:2,272
Mass (Da):257,236
Last modified:November 1, 1996 - v1
Checksum:i70D9200B9E0C87A1
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L29346 mRNA. Translation: AAA59206.1.
PIRiC54972.
UniGeneiMm.267517.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L29346 mRNA. Translation: AAA59206.1 .
PIRi C54972.
UniGenei Mm.267517.

3D structure databases

ProteinModelPortali Q61290.
SMRi Q61290. Positions 359-403, 1252-1279.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi Q61290. 2 interactions.

Protein family/group databases

TCDBi 1.A.1.11.3. the voltage-gated ion channel (vic) superfamily.

PTM databases

PhosphoSitei Q61290.

Proteomic databases

PaxDbi Q61290.
PRIDEi Q61290.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Organism-specific databases

MGIi MGI:106217. Cacna1e.

Phylogenomic databases

eggNOGi COG1226.
HOVERGENi HBG050763.
InParanoidi Q61290.
PhylomeDBi Q61290.

Miscellaneous databases

PROi Q61290.
SOURCEi Search...

Gene expression databases

Genevestigatori Q61290.

Family and domain databases

Gene3Di 1.20.120.350. 4 hits.
InterProi IPR027359. Channel_four-helix_dom.
IPR002048. EF_hand_dom.
IPR005821. Ion_trans_dom.
IPR014873. VDCC_a1su_IQ.
IPR005449. VDCC_R_a1su.
IPR002077. VDCCAlpha1.
[Graphical view ]
PANTHERi PTHR10037:SF57. PTHR10037:SF57. 1 hit.
Pfami PF08763. Ca_chan_IQ. 1 hit.
PF00520. Ion_trans. 4 hits.
[Graphical view ]
PRINTSi PR00167. CACHANNEL.
PR01633. RVDCCALPHA1.
SMARTi SM01062. Ca_chan_IQ. 1 hit.
[Graphical view ]
PROSITEi PS50222. EF_HAND_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Structure and functional characterization of neuronal alpha 1E calcium channel subtypes."
    Williams M.E., Marubio L.M., Deal C.R., Hans M., Brust P.F., Philipson L.H., Miller R.J., Johnson E.C., Harpold M.M., Ellis S.B.
    J. Biol. Chem. 269:22347-22357(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: BALB/c.
    Tissue: Brain.
  2. "Qualitative and quantitative analyses of protein phosphorylation in naive and stimulated mouse synaptosomal preparations."
    Munton R.P., Tweedie-Cullen R., Livingstone-Zatchej M., Weinandy F., Waidelich M., Longo D., Gehrig P., Potthast F., Rutishauser D., Gerrits B., Panse C., Schlapbach R., Mansuy I.M.
    Mol. Cell. Proteomics 6:283-293(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain cortex.

Entry informationi

Entry nameiCAC1E_MOUSE
AccessioniPrimary (citable) accession number: Q61290
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: November 1, 1996
Last modified: June 11, 2014
This is version 129 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi