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Protein

Transcription factor 12

Gene

Tcf12

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcriptional regulator. Involved in the initiation of neuronal differentiation. Activates transcription by binding to the E box (5'-CANNTG-3').1 Publication

GO - Molecular functioni

GO - Biological processi

  • positive regulation of gene expression Source: MGI
  • positive regulation of neuron differentiation Source: UniProtKB
  • positive regulation of transcription, DNA-templated Source: MGI
  • positive regulation of transcription from RNA polymerase II promoter Source: MGI

Keywordsi

Molecular functionDevelopmental protein, DNA-binding
Biological processDifferentiation, Neurogenesis, Transcription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-MMU-8939236. RUNX1 regulates transcription of genes involved in differentiation of HSCs.

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription factor 12
Short name:
TCF-12
Alternative name(s):
Class A helix-loop-helix transcription factor ME1
DNA-binding protein HTF4
E-box-binding protein
Transcription factor HTF-4
Gene namesi
Name:Tcf12
Synonyms:Alf1, Meb
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 9

Organism-specific databases

MGIiMGI:101877. Tcf12.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Mice are smaller than their wild-type littermates and fail to thrive 14 days after birth.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001272301 – 706Transcription factor 12Add BLAST706

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei47PhosphoserineBy similarity1
Modified residuei67PhosphoserineBy similarity1
Modified residuei79PhosphoserineBy similarity1
Modified residuei98PhosphoserineCombined sources1
Cross-linki110Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei116PhosphoserineBy similarity1
Modified residuei124PhosphoserineCombined sources1
Cross-linki181Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei313PhosphothreonineBy similarity1
Modified residuei333PhosphoserineBy similarity1
Cross-linki543Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei564PhosphoserineBy similarity1
Cross-linki574Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei581PhosphothreonineBy similarity1
Modified residuei582PhosphoserineBy similarity1
Modified residuei583PhosphoserineBy similarity1
Cross-linki633Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki677Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Isoform ALF1A (identifier: Q61286-2)
Modified residuei392PhosphoserineCombined sources1

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ61286.
PeptideAtlasiQ61286.
PRIDEiQ61286.

PTM databases

iPTMnetiQ61286.
PhosphoSitePlusiQ61286.

Expressioni

Tissue specificityi

Widely expressed.

Developmental stagei

Abundantly expressed during development of the central nervous system, in particular in proliferating neuroblasts and in cells at the initial stages of differentiation. Also expressed at high levels in morphogenetically active regions such as limb buds, somites and mesonephric tubules. Expression decreases once cellular differentiation is over.1 Publication

Gene expression databases

BgeeiENSMUSG00000032228.
CleanExiMM_TCF12.
ExpressionAtlasiQ61286. baseline and differential.
GenevisibleiQ61286. MM.

Interactioni

Subunit structurei

Efficient DNA binding requires dimerization with another bHLH protein. Forms homo- or heterooligomers with myogenin, E12 and ITF2 proteins and RUNX1T1 (By similarity). Interacts with PTF1A. Interacts with NEUROD2. Interacts with BHLHA9.By similarity2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Tal1P220912EBI-8006499,EBI-8006437

GO - Molecular functioni

Protein-protein interaction databases

BioGridi204000. 16 interactors.
CORUMiQ61286.
DIPiDIP-42840N.
IntActiQ61286. 4 interactors.
MINTiMINT-2567849.
STRINGi10090.ENSMUSP00000034755.

Structurei

3D structure databases

ProteinModelPortaliQ61286.
SMRiQ61286.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini601 – 654bHLHPROSITE-ProRule annotationAdd BLAST54

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni119 – 140Leucine-zipperAdd BLAST22
Regioni656 – 679Class A specific domainAdd BLAST24

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi181 – 188Nuclear localization signalSequence analysis8

Phylogenomic databases

eggNOGiKOG3910. Eukaryota.
ENOG410XYUA. LUCA.
GeneTreeiENSGT00510000046438.
HOGENOMiHOG000234180.
HOVERGENiHBG003854.
InParanoidiQ61286.
KOiK15603.
OMAiYYSFSAT.
OrthoDBiEOG091G07E2.
TreeFamiTF321672.

Family and domain databases

CDDicd00083. HLH. 1 hit.
Gene3Di4.10.280.10. 1 hit.
InterProiView protein in InterPro
IPR011598. bHLH_dom.
IPR036638. HLH_DNA-bd_sf.
PfamiView protein in Pfam
PF00010. HLH. 1 hit.
SMARTiView protein in SMART
SM00353. HLH. 1 hit.
SUPFAMiSSF47459. SSF47459. 1 hit.
PROSITEiView protein in PROSITE
PS50888. BHLH. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform ALF1B (identifier: Q61286-1) [UniParc]FASTAAdd to basket
Also known as: ME1A

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MNPQQQRMAA IGTDKELSDL LDFSAMFSPP VNSGKTRPTT LGSSQFSGSG
60 70 80 90 100
MDERGGTTSW GTSGQPSPSY DSSRGFTDSP HYSDHLNDSR LGTHEGLSPT
110 120 130 140 150
PFMNSNLIGK TSERGSFSLY SRDSGLSGCQ SSLLRQDLGL GSPAQLSSSG
160 170 180 190 200
KPGTPYYSFS ATSSRRRPLH DSVALDPLQA KKVRKVPPGL PSSVYAPSPN
210 220 230 240 250
SDDFNRESPS YPSPKPPTSM FASTFFMQDG THSSSDLWSS SNGMSQPGFG
260 270 280 290 300
GILGTSTSHM SQSSSYGSLH SHDRLSYPPH SVSPTDINTS LPPMSSFHRG
310 320 330 340 350
STSSSPYVAA SHTPPINGSD SILGTRGNAA GSSQTGDALG KALASIYSPD
360 370 380 390 400
HTSSSFPSNP STPVGSPSPL TGTSQWPRAG GQAPSSPSYE NSLHSLKNRV
410 420 430 440 450
EQQLHEHLQD AMSFLKDVCE QSRMEDRLDR LDDAIHVLRN HAVGPSTSLP
460 470 480 490 500
TSHSDIHSLL GPSHNASIGN LNSNYGGSSL VTNSRSASMV GTHREDSVSL
510 520 530 540 550
NGNHSVLSST VAASNTELNH KTPENFRGGV QNQSGSVVPT EIKTENKEKD
560 570 580 590 600
ENLHEPPSSD DMKSDDESSQ KDIKVSSRGR TSSTNEDEDL NPEQKIEREK
610 620 630 640 650
ERRMANNARE RLRVRDINEA FKELGRMCQL HLKSEKPQTK LLILHQAVAV
660 670 680 690 700
ILSLEQQVRE RNLNPKAACL KRREEEKVSA ASAEPPNTLP GAHPGLSEST

NPMGHL
Length:706
Mass (Da):75,811
Last modified:July 27, 2011 - v2
Checksum:i4394FE95A297F68C
GO
Isoform ALF1A (identifier: Q61286-2) [UniParc]FASTAAdd to basket
Also known as: ME1B

The sequence of this isoform differs from the canonical sequence as follows:
     397-420: Missing.

Show »
Length:682
Mass (Da):72,931
Checksum:iAE86072FA32586AA
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti416K → E in M97635 (PubMed:8261111).Curated1
Sequence conflicti537V → F in M97635 (PubMed:8261111).Curated1
Sequence conflicti537V → F in M97636 (PubMed:8261111).Curated1
Sequence conflicti687N → S in CAA46052 (PubMed:1321336).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_002105397 – 420Missing in isoform ALF1A. 1 PublicationAdd BLAST24

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X64840 mRNA. Translation: CAA46052.1.
M97635 mRNA. No translation available.
M97636 mRNA. No translation available.
AC093483 Genomic DNA. No translation available.
AC157575 Genomic DNA. No translation available.
AC159001 Genomic DNA. No translation available.
CCDSiCCDS23329.1. [Q61286-1]
CCDS72272.1. [Q61286-2]
PIRiC45020.
S19958.
RefSeqiNP_001240794.1. NM_001253865.1.
NP_035674.2. NM_011544.3. [Q61286-1]
XP_006511057.1. XM_006510994.3. [Q61286-1]
XP_006511060.1. XM_006510997.2. [Q61286-2]
UniGeneiMm.171615.
Mm.480117.

Genome annotation databases

EnsembliENSMUST00000034755; ENSMUSP00000034755; ENSMUSG00000032228. [Q61286-2]
ENSMUST00000183404; ENSMUSP00000139365; ENSMUSG00000032228. [Q61286-1]
ENSMUST00000184783; ENSMUSP00000139364; ENSMUSG00000032228. [Q61286-1]
ENSMUST00000185117; ENSMUSP00000138925; ENSMUSG00000032228. [Q61286-2]
GeneIDi21406.
KEGGimmu:21406.
UCSCiuc009qpf.2. mouse. [Q61286-1]
uc009qpg.2. mouse. [Q61286-2]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiHTF4_MOUSE
AccessioniPrimary (citable) accession number: Q61286
Secondary accession number(s): E9QPN4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: July 27, 2011
Last modified: October 25, 2017
This is version 152 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot