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Q61286

- HTF4_MOUSE

UniProt

Q61286 - HTF4_MOUSE

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Protein
Transcription factor 12
Gene
Tcf12, Alf1, Meb
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Transcriptional regulator. Involved in the initiation of neuronal differentiation. Activates transcription by binding to the E box (5'-CANNTG-3').1 Publication

GO - Molecular functioni

  1. DNA binding Source: MGI
  2. E-box binding Source: UniProtKB
  3. protein binding Source: UniProtKB
  4. protein heterodimerization activity Source: UniProtKB

GO - Biological processi

  1. positive regulation of gene expression Source: MGI
  2. positive regulation of neuron differentiation Source: UniProtKB
  3. positive regulation of transcription, DNA-templated Source: MGI
  4. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Differentiation, Neurogenesis, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription factor 12
Short name:
TCF-12
Alternative name(s):
Class A helix-loop-helix transcription factor ME1
DNA-binding protein HTF4
E-box-binding protein
Transcription factor HTF-4
Gene namesi
Name:Tcf12
Synonyms:Alf1, Meb
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 9

Organism-specific databases

MGIiMGI:101877. Tcf12.

Subcellular locationi

GO - Cellular componenti

  1. nucleus Source: UniProtKB-SubCell
  2. transcription factor complex Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Mice are smaller than their wild-type littermates and fail to thrive 14 days after birth.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 706706Transcription factor 12
PRO_0000127230Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei67 – 671Phosphoserine By similarity
Modified residuei313 – 3131Phosphothreonine By similarity
Modified residuei333 – 3331Phosphoserine By similarity
Modified residuei581 – 5811Phosphothreonine By similarity
Modified residuei583 – 5831Phosphoserine By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ61286.
PRIDEiQ61286.

PTM databases

PhosphoSiteiQ61286.

Expressioni

Tissue specificityi

Widely expressed.

Developmental stagei

Abundantly expressed during development of the central nervous system, in particular in proliferating neuroblasts and in cells at the initial stages of differentiation. Also expressed at high levels in morphogenetically active regions such as limb buds, somites and mesonephric tubules. Expression decreases once cellular differentiation is over.1 Publication

Gene expression databases

BgeeiQ61286.
CleanExiMM_TCF12.
GenevestigatoriQ61286.

Interactioni

Subunit structurei

Efficient DNA binding requires dimerization with another bHLH protein. Forms homo- or heterooligomers with myogenin, E12 and ITF2 proteins and RUNX1T1 By similarity. Interacts with PTF1A. Interacts with NEUROD2.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Tal1P220912EBI-8006499,EBI-8006437

Protein-protein interaction databases

BioGridi204000. 16 interactions.
DIPiDIP-42840N.
IntActiQ61286. 3 interactions.
MINTiMINT-2567849.

Structurei

3D structure databases

ProteinModelPortaliQ61286.
SMRiQ61286. Positions 602-661.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini601 – 65454bHLH
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni119 – 14022Leucine-zipper
Add
BLAST
Regioni656 – 67924Class A specific domain
Add
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi181 – 1888Nuclear localization signal Reviewed prediction

Sequence similaritiesi

Phylogenomic databases

eggNOGiNOG282899.
GeneTreeiENSGT00510000046438.
HOGENOMiHOG000234180.
HOVERGENiHBG003854.
InParanoidiQ61286.
KOiK15603.
OMAiYYSFSAT.
OrthoDBiEOG72G16Q.
TreeFamiTF321672.

Family and domain databases

Gene3Di4.10.280.10. 1 hit.
InterProiIPR011598. bHLH_dom.
[Graphical view]
PfamiPF00010. HLH. 1 hit.
[Graphical view]
SMARTiSM00353. HLH. 1 hit.
[Graphical view]
SUPFAMiSSF47459. SSF47459. 1 hit.
PROSITEiPS50888. BHLH. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform ALF1B (identifier: Q61286-1) [UniParc]FASTAAdd to Basket

Also known as: ME1A

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MNPQQQRMAA IGTDKELSDL LDFSAMFSPP VNSGKTRPTT LGSSQFSGSG    50
MDERGGTTSW GTSGQPSPSY DSSRGFTDSP HYSDHLNDSR LGTHEGLSPT 100
PFMNSNLIGK TSERGSFSLY SRDSGLSGCQ SSLLRQDLGL GSPAQLSSSG 150
KPGTPYYSFS ATSSRRRPLH DSVALDPLQA KKVRKVPPGL PSSVYAPSPN 200
SDDFNRESPS YPSPKPPTSM FASTFFMQDG THSSSDLWSS SNGMSQPGFG 250
GILGTSTSHM SQSSSYGSLH SHDRLSYPPH SVSPTDINTS LPPMSSFHRG 300
STSSSPYVAA SHTPPINGSD SILGTRGNAA GSSQTGDALG KALASIYSPD 350
HTSSSFPSNP STPVGSPSPL TGTSQWPRAG GQAPSSPSYE NSLHSLKNRV 400
EQQLHEHLQD AMSFLKDVCE QSRMEDRLDR LDDAIHVLRN HAVGPSTSLP 450
TSHSDIHSLL GPSHNASIGN LNSNYGGSSL VTNSRSASMV GTHREDSVSL 500
NGNHSVLSST VAASNTELNH KTPENFRGGV QNQSGSVVPT EIKTENKEKD 550
ENLHEPPSSD DMKSDDESSQ KDIKVSSRGR TSSTNEDEDL NPEQKIEREK 600
ERRMANNARE RLRVRDINEA FKELGRMCQL HLKSEKPQTK LLILHQAVAV 650
ILSLEQQVRE RNLNPKAACL KRREEEKVSA ASAEPPNTLP GAHPGLSEST 700
NPMGHL 706
Length:706
Mass (Da):75,811
Last modified:July 27, 2011 - v2
Checksum:i4394FE95A297F68C
GO
Isoform ALF1A (identifier: Q61286-2) [UniParc]FASTAAdd to Basket

Also known as: ME1B

The sequence of this isoform differs from the canonical sequence as follows:
     397-420: Missing.

Show »
Length:682
Mass (Da):72,931
Checksum:iAE86072FA32586AA
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei397 – 42024Missing in isoform ALF1A.
VSP_002105Add
BLAST

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti416 – 4161K → E in M97635. 1 Publication
Sequence conflicti537 – 5371V → F in M97635. 1 Publication
Sequence conflicti537 – 5371V → F in M97636. 1 Publication
Sequence conflicti687 – 6871N → S in CAA46052. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X64840 mRNA. Translation: CAA46052.1.
M97635 mRNA. No translation available.
M97636 mRNA. No translation available.
AC093483 Genomic DNA. No translation available.
AC157575 Genomic DNA. No translation available.
AC159001 Genomic DNA. No translation available.
CCDSiCCDS23329.1. [Q61286-1]
CCDS72272.1. [Q61286-2]
PIRiC45020.
S19958.
RefSeqiNP_001240794.1. NM_001253865.1.
NP_035674.2. NM_011544.3. [Q61286-1]
XP_006511057.1. XM_006510994.1. [Q61286-1]
XP_006511060.1. XM_006510997.1. [Q61286-2]
UniGeneiMm.171615.
Mm.480117.

Genome annotation databases

EnsembliENSMUST00000034755; ENSMUSP00000034755; ENSMUSG00000032228. [Q61286-2]
ENSMUST00000183404; ENSMUSP00000139365; ENSMUSG00000032228. [Q61286-1]
ENSMUST00000184783; ENSMUSP00000139364; ENSMUSG00000032228. [Q61286-1]
ENSMUST00000185117; ENSMUSP00000138925; ENSMUSG00000032228. [Q61286-2]
GeneIDi21406.
KEGGimmu:21406.
UCSCiuc009qpf.2. mouse. [Q61286-1]
uc009qpi.2. mouse. [Q61286-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X64840 mRNA. Translation: CAA46052.1 .
M97635 mRNA. No translation available.
M97636 mRNA. No translation available.
AC093483 Genomic DNA. No translation available.
AC157575 Genomic DNA. No translation available.
AC159001 Genomic DNA. No translation available.
CCDSi CCDS23329.1. [Q61286-1 ]
CCDS72272.1. [Q61286-2 ]
PIRi C45020.
S19958.
RefSeqi NP_001240794.1. NM_001253865.1.
NP_035674.2. NM_011544.3. [Q61286-1 ]
XP_006511057.1. XM_006510994.1. [Q61286-1 ]
XP_006511060.1. XM_006510997.1. [Q61286-2 ]
UniGenei Mm.171615.
Mm.480117.

3D structure databases

ProteinModelPortali Q61286.
SMRi Q61286. Positions 602-661.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 204000. 16 interactions.
DIPi DIP-42840N.
IntActi Q61286. 3 interactions.
MINTi MINT-2567849.

PTM databases

PhosphoSitei Q61286.

Proteomic databases

PaxDbi Q61286.
PRIDEi Q61286.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000034755 ; ENSMUSP00000034755 ; ENSMUSG00000032228 . [Q61286-2 ]
ENSMUST00000183404 ; ENSMUSP00000139365 ; ENSMUSG00000032228 . [Q61286-1 ]
ENSMUST00000184783 ; ENSMUSP00000139364 ; ENSMUSG00000032228 . [Q61286-1 ]
ENSMUST00000185117 ; ENSMUSP00000138925 ; ENSMUSG00000032228 . [Q61286-2 ]
GeneIDi 21406.
KEGGi mmu:21406.
UCSCi uc009qpf.2. mouse. [Q61286-1 ]
uc009qpi.2. mouse. [Q61286-2 ]

Organism-specific databases

CTDi 6938.
MGIi MGI:101877. Tcf12.

Phylogenomic databases

eggNOGi NOG282899.
GeneTreei ENSGT00510000046438.
HOGENOMi HOG000234180.
HOVERGENi HBG003854.
InParanoidi Q61286.
KOi K15603.
OMAi YYSFSAT.
OrthoDBi EOG72G16Q.
TreeFami TF321672.

Miscellaneous databases

ChiTaRSi TCF12. mouse.
NextBioi 300676.
PROi Q61286.
SOURCEi Search...

Gene expression databases

Bgeei Q61286.
CleanExi MM_TCF12.
Genevestigatori Q61286.

Family and domain databases

Gene3Di 4.10.280.10. 1 hit.
InterProi IPR011598. bHLH_dom.
[Graphical view ]
Pfami PF00010. HLH. 1 hit.
[Graphical view ]
SMARTi SM00353. HLH. 1 hit.
[Graphical view ]
SUPFAMi SSF47459. SSF47459. 1 hit.
PROSITEi PS50888. BHLH. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Murine helix-loop-helix transcriptional activator proteins binding to the E-box motif of the Akv murine leukemia virus enhancer identified by cDNA cloning."
    Nielsen A.L., Pallisgaard N., Pedersen F.S., Joergensen P.
    Mol. Cell. Biol. 12:3449-3459(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS ALF1A AND ALF1B).
  2. "ME1 and GE1: basic helix-loop-helix transcription factors expressed at high levels in the developing nervous system and in morphogenetically active regions."
    Neuman T., Keen A., Knapik E., Shain D., Ross M., Nornes H.O., Zuber M.X.
    Eur. J. Neurosci. 5:311-318(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: BALB/c.
    Tissue: Cerebellum.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  4. "The role of PTF1-P48 in pancreatic acinar gene expression."
    Rose S.D., Swift G.H., Peyton M.J., Hammer R.E., MacDonald R.J.
    J. Biol. Chem. 276:44018-44026(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH PTF1A.
  5. "E protein dosage influences brain development more than family member identity."
    Ravanpay A.C., Olson J.M.
    J. Neurosci. Res. 86:1472-1481(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH NEUROD2, DNA-BINDING, DISRUPTION PHENOTYPE, DEVELOPMENTAL STAGE.

Entry informationi

Entry nameiHTF4_MOUSE
AccessioniPrimary (citable) accession number: Q61286
Secondary accession number(s): E9QPN4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: July 27, 2011
Last modified: July 9, 2014
This is version 125 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi