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Q61286

- HTF4_MOUSE

UniProt

Q61286 - HTF4_MOUSE

Protein

Transcription factor 12

Gene

Tcf12

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 126 (01 Oct 2014)
      Sequence version 2 (27 Jul 2011)
      Previous versions | rss
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    Functioni

    Transcriptional regulator. Involved in the initiation of neuronal differentiation. Activates transcription by binding to the E box (5'-CANNTG-3').1 Publication

    GO - Molecular functioni

    1. DNA binding Source: MGI
    2. E-box binding Source: UniProtKB
    3. protein binding Source: UniProtKB
    4. protein heterodimerization activity Source: UniProtKB

    GO - Biological processi

    1. positive regulation of gene expression Source: MGI
    2. positive regulation of neuron differentiation Source: UniProtKB
    3. positive regulation of transcription, DNA-templated Source: MGI
    4. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Molecular functioni

    Developmental protein

    Keywords - Biological processi

    Differentiation, Neurogenesis, Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Transcription factor 12
    Short name:
    TCF-12
    Alternative name(s):
    Class A helix-loop-helix transcription factor ME1
    DNA-binding protein HTF4
    E-box-binding protein
    Transcription factor HTF-4
    Gene namesi
    Name:Tcf12
    Synonyms:Alf1, Meb
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 9

    Organism-specific databases

    MGIiMGI:101877. Tcf12.

    Subcellular locationi

    GO - Cellular componenti

    1. nucleus Source: UniProtKB-SubCell
    2. transcription factor complex Source: MGI

    Keywords - Cellular componenti

    Nucleus

    Pathology & Biotechi

    Disruption phenotypei

    Mice are smaller than their wild-type littermates and fail to thrive 14 days after birth.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 706706Transcription factor 12PRO_0000127230Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei67 – 671PhosphoserineBy similarity
    Modified residuei313 – 3131PhosphothreonineBy similarity
    Modified residuei333 – 3331PhosphoserineBy similarity
    Modified residuei581 – 5811PhosphothreonineBy similarity
    Modified residuei583 – 5831PhosphoserineBy similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiQ61286.
    PRIDEiQ61286.

    PTM databases

    PhosphoSiteiQ61286.

    Expressioni

    Tissue specificityi

    Widely expressed.

    Developmental stagei

    Abundantly expressed during development of the central nervous system, in particular in proliferating neuroblasts and in cells at the initial stages of differentiation. Also expressed at high levels in morphogenetically active regions such as limb buds, somites and mesonephric tubules. Expression decreases once cellular differentiation is over.1 Publication

    Gene expression databases

    BgeeiQ61286.
    CleanExiMM_TCF12.
    GenevestigatoriQ61286.

    Interactioni

    Subunit structurei

    Efficient DNA binding requires dimerization with another bHLH protein. Forms homo- or heterooligomers with myogenin, E12 and ITF2 proteins and RUNX1T1 By similarity. Interacts with PTF1A. Interacts with NEUROD2.By similarity2 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    Tal1P220912EBI-8006499,EBI-8006437

    Protein-protein interaction databases

    BioGridi204000. 16 interactions.
    DIPiDIP-42840N.
    IntActiQ61286. 3 interactions.
    MINTiMINT-2567849.

    Structurei

    3D structure databases

    ProteinModelPortaliQ61286.
    SMRiQ61286. Positions 602-661.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini601 – 65454bHLHPROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni119 – 14022Leucine-zipperAdd
    BLAST
    Regioni656 – 67924Class A specific domainAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi181 – 1888Nuclear localization signalSequence Analysis

    Sequence similaritiesi

    Contains 1 bHLH (basic helix-loop-helix) domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiNOG282899.
    GeneTreeiENSGT00510000046438.
    HOGENOMiHOG000234180.
    HOVERGENiHBG003854.
    InParanoidiQ61286.
    KOiK15603.
    OMAiYYSFSAT.
    OrthoDBiEOG72G16Q.
    TreeFamiTF321672.

    Family and domain databases

    Gene3Di4.10.280.10. 1 hit.
    InterProiIPR011598. bHLH_dom.
    [Graphical view]
    PfamiPF00010. HLH. 1 hit.
    [Graphical view]
    SMARTiSM00353. HLH. 1 hit.
    [Graphical view]
    SUPFAMiSSF47459. SSF47459. 1 hit.
    PROSITEiPS50888. BHLH. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform ALF1B (identifier: Q61286-1) [UniParc]FASTAAdd to Basket

    Also known as: ME1A

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MNPQQQRMAA IGTDKELSDL LDFSAMFSPP VNSGKTRPTT LGSSQFSGSG    50
    MDERGGTTSW GTSGQPSPSY DSSRGFTDSP HYSDHLNDSR LGTHEGLSPT 100
    PFMNSNLIGK TSERGSFSLY SRDSGLSGCQ SSLLRQDLGL GSPAQLSSSG 150
    KPGTPYYSFS ATSSRRRPLH DSVALDPLQA KKVRKVPPGL PSSVYAPSPN 200
    SDDFNRESPS YPSPKPPTSM FASTFFMQDG THSSSDLWSS SNGMSQPGFG 250
    GILGTSTSHM SQSSSYGSLH SHDRLSYPPH SVSPTDINTS LPPMSSFHRG 300
    STSSSPYVAA SHTPPINGSD SILGTRGNAA GSSQTGDALG KALASIYSPD 350
    HTSSSFPSNP STPVGSPSPL TGTSQWPRAG GQAPSSPSYE NSLHSLKNRV 400
    EQQLHEHLQD AMSFLKDVCE QSRMEDRLDR LDDAIHVLRN HAVGPSTSLP 450
    TSHSDIHSLL GPSHNASIGN LNSNYGGSSL VTNSRSASMV GTHREDSVSL 500
    NGNHSVLSST VAASNTELNH KTPENFRGGV QNQSGSVVPT EIKTENKEKD 550
    ENLHEPPSSD DMKSDDESSQ KDIKVSSRGR TSSTNEDEDL NPEQKIEREK 600
    ERRMANNARE RLRVRDINEA FKELGRMCQL HLKSEKPQTK LLILHQAVAV 650
    ILSLEQQVRE RNLNPKAACL KRREEEKVSA ASAEPPNTLP GAHPGLSEST 700
    NPMGHL 706
    Length:706
    Mass (Da):75,811
    Last modified:July 27, 2011 - v2
    Checksum:i4394FE95A297F68C
    GO
    Isoform ALF1A (identifier: Q61286-2) [UniParc]FASTAAdd to Basket

    Also known as: ME1B

    The sequence of this isoform differs from the canonical sequence as follows:
         397-420: Missing.

    Show »
    Length:682
    Mass (Da):72,931
    Checksum:iAE86072FA32586AA
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti416 – 4161K → E in M97635. (PubMed:8261111)Curated
    Sequence conflicti537 – 5371V → F in M97635. (PubMed:8261111)Curated
    Sequence conflicti537 – 5371V → F in M97636. (PubMed:8261111)Curated
    Sequence conflicti687 – 6871N → S in CAA46052. (PubMed:1321336)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei397 – 42024Missing in isoform ALF1A. 1 PublicationVSP_002105Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X64840 mRNA. Translation: CAA46052.1.
    M97635 mRNA. No translation available.
    M97636 mRNA. No translation available.
    AC093483 Genomic DNA. No translation available.
    AC157575 Genomic DNA. No translation available.
    AC159001 Genomic DNA. No translation available.
    CCDSiCCDS23329.1. [Q61286-1]
    CCDS72272.1. [Q61286-2]
    PIRiC45020.
    S19958.
    RefSeqiNP_001240794.1. NM_001253865.1.
    NP_035674.2. NM_011544.3. [Q61286-1]
    XP_006511057.1. XM_006510994.1. [Q61286-1]
    XP_006511060.1. XM_006510997.1. [Q61286-2]
    UniGeneiMm.171615.
    Mm.480117.

    Genome annotation databases

    EnsembliENSMUST00000034755; ENSMUSP00000034755; ENSMUSG00000032228. [Q61286-2]
    ENSMUST00000183404; ENSMUSP00000139365; ENSMUSG00000032228. [Q61286-1]
    ENSMUST00000184783; ENSMUSP00000139364; ENSMUSG00000032228. [Q61286-1]
    ENSMUST00000185117; ENSMUSP00000138925; ENSMUSG00000032228. [Q61286-2]
    GeneIDi21406.
    KEGGimmu:21406.
    UCSCiuc009qpf.2. mouse. [Q61286-1]
    uc009qpi.2. mouse. [Q61286-2]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X64840 mRNA. Translation: CAA46052.1 .
    M97635 mRNA. No translation available.
    M97636 mRNA. No translation available.
    AC093483 Genomic DNA. No translation available.
    AC157575 Genomic DNA. No translation available.
    AC159001 Genomic DNA. No translation available.
    CCDSi CCDS23329.1. [Q61286-1 ]
    CCDS72272.1. [Q61286-2 ]
    PIRi C45020.
    S19958.
    RefSeqi NP_001240794.1. NM_001253865.1.
    NP_035674.2. NM_011544.3. [Q61286-1 ]
    XP_006511057.1. XM_006510994.1. [Q61286-1 ]
    XP_006511060.1. XM_006510997.1. [Q61286-2 ]
    UniGenei Mm.171615.
    Mm.480117.

    3D structure databases

    ProteinModelPortali Q61286.
    SMRi Q61286. Positions 602-661.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 204000. 16 interactions.
    DIPi DIP-42840N.
    IntActi Q61286. 3 interactions.
    MINTi MINT-2567849.

    PTM databases

    PhosphoSitei Q61286.

    Proteomic databases

    PaxDbi Q61286.
    PRIDEi Q61286.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000034755 ; ENSMUSP00000034755 ; ENSMUSG00000032228 . [Q61286-2 ]
    ENSMUST00000183404 ; ENSMUSP00000139365 ; ENSMUSG00000032228 . [Q61286-1 ]
    ENSMUST00000184783 ; ENSMUSP00000139364 ; ENSMUSG00000032228 . [Q61286-1 ]
    ENSMUST00000185117 ; ENSMUSP00000138925 ; ENSMUSG00000032228 . [Q61286-2 ]
    GeneIDi 21406.
    KEGGi mmu:21406.
    UCSCi uc009qpf.2. mouse. [Q61286-1 ]
    uc009qpi.2. mouse. [Q61286-2 ]

    Organism-specific databases

    CTDi 6938.
    MGIi MGI:101877. Tcf12.

    Phylogenomic databases

    eggNOGi NOG282899.
    GeneTreei ENSGT00510000046438.
    HOGENOMi HOG000234180.
    HOVERGENi HBG003854.
    InParanoidi Q61286.
    KOi K15603.
    OMAi YYSFSAT.
    OrthoDBi EOG72G16Q.
    TreeFami TF321672.

    Miscellaneous databases

    ChiTaRSi TCF12. mouse.
    NextBioi 300676.
    PROi Q61286.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q61286.
    CleanExi MM_TCF12.
    Genevestigatori Q61286.

    Family and domain databases

    Gene3Di 4.10.280.10. 1 hit.
    InterProi IPR011598. bHLH_dom.
    [Graphical view ]
    Pfami PF00010. HLH. 1 hit.
    [Graphical view ]
    SMARTi SM00353. HLH. 1 hit.
    [Graphical view ]
    SUPFAMi SSF47459. SSF47459. 1 hit.
    PROSITEi PS50888. BHLH. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Murine helix-loop-helix transcriptional activator proteins binding to the E-box motif of the Akv murine leukemia virus enhancer identified by cDNA cloning."
      Nielsen A.L., Pallisgaard N., Pedersen F.S., Joergensen P.
      Mol. Cell. Biol. 12:3449-3459(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS ALF1A AND ALF1B).
    2. "ME1 and GE1: basic helix-loop-helix transcription factors expressed at high levels in the developing nervous system and in morphogenetically active regions."
      Neuman T., Keen A., Knapik E., Shain D., Ross M., Nornes H.O., Zuber M.X.
      Eur. J. Neurosci. 5:311-318(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: BALB/c.
      Tissue: Cerebellum.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    4. "The role of PTF1-P48 in pancreatic acinar gene expression."
      Rose S.D., Swift G.H., Peyton M.J., Hammer R.E., MacDonald R.J.
      J. Biol. Chem. 276:44018-44026(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH PTF1A.
    5. "E protein dosage influences brain development more than family member identity."
      Ravanpay A.C., Olson J.M.
      J. Neurosci. Res. 86:1472-1481(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH NEUROD2, DNA-BINDING, DISRUPTION PHENOTYPE, DEVELOPMENTAL STAGE.

    Entry informationi

    Entry nameiHTF4_MOUSE
    AccessioniPrimary (citable) accession number: Q61286
    Secondary accession number(s): E9QPN4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 30, 2000
    Last sequence update: July 27, 2011
    Last modified: October 1, 2014
    This is version 126 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3