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Q61286 (HTF4_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 122. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Transcription factor 12

Short name=TCF-12
Alternative name(s):
Class A helix-loop-helix transcription factor ME1
DNA-binding protein HTF4
E-box-binding protein
Transcription factor HTF-4
Gene names
Name:Tcf12
Synonyms:Alf1, Meb
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length706 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Transcriptional regulator. Involved in the initiation of neuronal differentiation. Activates transcription by binding to the E box (5'-CANNTG-3'). Ref.5

Subunit structure

Efficient DNA binding requires dimerization with another bHLH protein. Forms homo- or heterooligomers with myogenin, E12 and ITF2 proteins and RUNX1T1 By similarity. Interacts with PTF1A. Interacts with NEUROD2. Ref.4 Ref.5

Subcellular location

Nucleus.

Tissue specificity

Widely expressed.

Developmental stage

Abundantly expressed during development of the central nervous system, in particular in proliferating neuroblasts and in cells at the initial stages of differentiation. Also expressed at high levels in morphogenetically active regions such as limb buds, somites and mesonephric tubules. Expression decreases once cellular differentiation is over. Ref.5

Disruption phenotype

Mice are smaller than their wild-type littermates and fail to thrive 14 days after birth. Ref.5

Sequence similarities

Contains 1 bHLH (basic helix-loop-helix) domain.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Tal1P220912EBI-8006499,EBI-8006437

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform ALF1B (identifier: Q61286-1)

Also known as: ME1A;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform ALF1A (identifier: Q61286-2)

Also known as: ME1B;

The sequence of this isoform differs from the canonical sequence as follows:
     397-420: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 706706Transcription factor 12
PRO_0000127230

Regions

Domain601 – 65454bHLH
Region119 – 14022Leucine-zipper
Region656 – 67924Class A specific domain
Motif181 – 1888Nuclear localization signal Potential

Amino acid modifications

Modified residue671Phosphoserine By similarity
Modified residue3131Phosphothreonine By similarity
Modified residue3331Phosphoserine By similarity
Modified residue5811Phosphothreonine By similarity
Modified residue5831Phosphoserine By similarity

Natural variations

Alternative sequence397 – 42024Missing in isoform ALF1A.
VSP_002105

Experimental info

Sequence conflict4161K → E in M97635. Ref.2
Sequence conflict5371V → F in M97635. Ref.2
Sequence conflict5371V → F in M97636. Ref.2
Sequence conflict6871N → S in CAA46052. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform ALF1B (ME1A) [UniParc].

Last modified July 27, 2011. Version 2.
Checksum: 4394FE95A297F68C

FASTA70675,811
        10         20         30         40         50         60 
MNPQQQRMAA IGTDKELSDL LDFSAMFSPP VNSGKTRPTT LGSSQFSGSG MDERGGTTSW 

        70         80         90        100        110        120 
GTSGQPSPSY DSSRGFTDSP HYSDHLNDSR LGTHEGLSPT PFMNSNLIGK TSERGSFSLY 

       130        140        150        160        170        180 
SRDSGLSGCQ SSLLRQDLGL GSPAQLSSSG KPGTPYYSFS ATSSRRRPLH DSVALDPLQA 

       190        200        210        220        230        240 
KKVRKVPPGL PSSVYAPSPN SDDFNRESPS YPSPKPPTSM FASTFFMQDG THSSSDLWSS 

       250        260        270        280        290        300 
SNGMSQPGFG GILGTSTSHM SQSSSYGSLH SHDRLSYPPH SVSPTDINTS LPPMSSFHRG 

       310        320        330        340        350        360 
STSSSPYVAA SHTPPINGSD SILGTRGNAA GSSQTGDALG KALASIYSPD HTSSSFPSNP 

       370        380        390        400        410        420 
STPVGSPSPL TGTSQWPRAG GQAPSSPSYE NSLHSLKNRV EQQLHEHLQD AMSFLKDVCE 

       430        440        450        460        470        480 
QSRMEDRLDR LDDAIHVLRN HAVGPSTSLP TSHSDIHSLL GPSHNASIGN LNSNYGGSSL 

       490        500        510        520        530        540 
VTNSRSASMV GTHREDSVSL NGNHSVLSST VAASNTELNH KTPENFRGGV QNQSGSVVPT 

       550        560        570        580        590        600 
EIKTENKEKD ENLHEPPSSD DMKSDDESSQ KDIKVSSRGR TSSTNEDEDL NPEQKIEREK 

       610        620        630        640        650        660 
ERRMANNARE RLRVRDINEA FKELGRMCQL HLKSEKPQTK LLILHQAVAV ILSLEQQVRE 

       670        680        690        700 
RNLNPKAACL KRREEEKVSA ASAEPPNTLP GAHPGLSEST NPMGHL 

« Hide

Isoform ALF1A (ME1B) [UniParc].

Checksum: AE86072FA32586AA
Show »

FASTA68272,931

References

« Hide 'large scale' references
[1]"Murine helix-loop-helix transcriptional activator proteins binding to the E-box motif of the Akv murine leukemia virus enhancer identified by cDNA cloning."
Nielsen A.L., Pallisgaard N., Pedersen F.S., Joergensen P.
Mol. Cell. Biol. 12:3449-3459(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS ALF1A AND ALF1B).
[2]"ME1 and GE1: basic helix-loop-helix transcription factors expressed at high levels in the developing nervous system and in morphogenetically active regions."
Neuman T., Keen A., Knapik E., Shain D., Ross M., Nornes H.O., Zuber M.X.
Eur. J. Neurosci. 5:311-318(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: BALB/c.
Tissue: Cerebellum.
[3]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[4]"The role of PTF1-P48 in pancreatic acinar gene expression."
Rose S.D., Swift G.H., Peyton M.J., Hammer R.E., MacDonald R.J.
J. Biol. Chem. 276:44018-44026(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH PTF1A.
[5]"E protein dosage influences brain development more than family member identity."
Ravanpay A.C., Olson J.M.
J. Neurosci. Res. 86:1472-1481(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH NEUROD2, DNA-BINDING, DISRUPTION PHENOTYPE, DEVELOPMENTAL STAGE.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X64840 mRNA. Translation: CAA46052.1.
M97635 mRNA. No translation available.
M97636 mRNA. No translation available.
AC093483 Genomic DNA. No translation available.
AC157575 Genomic DNA. No translation available.
AC159001 Genomic DNA. No translation available.
PIRC45020.
S19958.
RefSeqNP_001240794.1. NM_001253865.1.
NP_035674.2. NM_011544.3.
XP_006511057.1. XM_006510994.1.
XP_006511060.1. XM_006510997.1.
UniGeneMm.171615.
Mm.480117.

3D structure databases

ProteinModelPortalQ61286.
SMRQ61286. Positions 602-661.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid204000. 16 interactions.
DIPDIP-42840N.
IntActQ61286. 3 interactions.
MINTMINT-2567849.

PTM databases

PhosphoSiteQ61286.

Proteomic databases

PaxDbQ61286.
PRIDEQ61286.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000034755; ENSMUSP00000034755; ENSMUSG00000032228. [Q61286-2]
ENSMUST00000183404; ENSMUSP00000139365; ENSMUSG00000032228. [Q61286-1]
ENSMUST00000184783; ENSMUSP00000139364; ENSMUSG00000032228. [Q61286-1]
ENSMUST00000185117; ENSMUSP00000138925; ENSMUSG00000032228. [Q61286-2]
GeneID21406.
KEGGmmu:21406.
UCSCuc009qpf.2. mouse. [Q61286-1]
uc009qpi.2. mouse. [Q61286-2]

Organism-specific databases

CTD6938.
MGIMGI:101877. Tcf12.

Phylogenomic databases

eggNOGNOG282899.
GeneTreeENSGT00510000046438.
HOGENOMHOG000234180.
HOVERGENHBG003854.
InParanoidQ61286.
KOK15603.
OMAYYSFSAT.
OrthoDBEOG72G16Q.
TreeFamTF321672.

Gene expression databases

BgeeQ61286.
CleanExMM_TCF12.
GenevestigatorQ61286.

Family and domain databases

Gene3D4.10.280.10. 1 hit.
InterProIPR011598. bHLH_dom.
[Graphical view]
PfamPF00010. HLH. 1 hit.
[Graphical view]
SMARTSM00353. HLH. 1 hit.
[Graphical view]
SUPFAMSSF47459. SSF47459. 1 hit.
PROSITEPS50888. BHLH. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSTCF12. mouse.
NextBio300676.
PROQ61286.
SOURCESearch...

Entry information

Entry nameHTF4_MOUSE
AccessionPrimary (citable) accession number: Q61286
Secondary accession number(s): E9QPN4
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: July 27, 2011
Last modified: April 16, 2014
This is version 122 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot