Reviewed,
UniProtKB/Swiss-Prot Q61286 (HTF4_MOUSE)
Last modified
June 16, 2009.
Version 76.
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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents
Names and origin
| Protein names | Recommended name: Transcription factor 12 Alternative name(s): Transcription factor HTF-4 E-box-binding protein DNA-binding protein HTF4 Class A helix-loop-helix transcription factor ME1 | ||||
| Gene names |
| ||||
| Organism | Mus musculus (Mouse) | ||||
| Taxonomic identifier | 10090 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus |
Protein attributes
| Sequence length | 706 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Binds specifically to oligomers of E-box motifs. May play important roles during development of the nervous system as well as in other organ systems. |
| Subunit structure | Efficient DNA binding requires dimerization with another bHLH protein. Forms homo- or heterooligomers with myogenin, E12 and ITF2 proteins and RUNX1T1 By similarity. Interacts with PTF1A. |
| Subcellular location | |
| Tissue specificity | Widely expressed. |
| Developmental stage | Abundantly expressed during development of the central nervous system, in particular in proliferating neuroblasts and in cells at the initial stages of differentiation. Also expressed at high levels in morphogenetically active regions such as limb buds, somites and mesonephric tubules. Expression decreases once cellular differentiation is over. |
| Post-translational modification | Phosphorylated upon DNA damage, probably by ATM or ATR By similarity. |
| Sequence similarities | Contains 1 basic helix-loop-helix (bHLH) domain. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Transcription Transcription regulation |
| Cellular component | Nucleus |
| Coding sequence diversity | Alternative splicing |
| Ligand | DNA-binding |
| Molecular function | Developmental protein |
| PTM | Phosphoprotein |
| Gene Ontology (GO) | |
| Biological process | multicellular organismal development Inferred from electronic annotation. Source: UniProtKB-KW regulation of transcription from RNA polymerase II promoter Ref.1Inferred from direct assay. Source: MGI transcriptionInferred from electronic annotation. Source: UniProtKB-KW |
| Cellular component | transcription factor complex Inferred from direct assay. Source: MGI |
| Molecular function | DNA binding Ref.1 Inferred from direct assay. Source: MGI protein bindingInferred from physical interaction. Source: UniProtKB transcription activator activity Ref.1Inferred from direct assay. Source: MGI |
| Complete GO annotation... | |
Alternative products
| This entry describes 2 isoforms produced by alternative splicing. [Align] [Select] | ||||||
| Isoform ALF1B (identifier: Q61286-1) Also known as: ME1A; This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. | ||||||
| Isoform ALF1A (identifier: Q61286-2) Also known as: ME1B; The sequence of this isoform differs from the canonical sequence as follows: 397-420: Missing. |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 706 | 706 | Transcription factor 12 | PRO_0000127230 | |||||
Regions | |||||||||
| Domain | 119 – 140 | 22 | Leucine-zipper Potential | ||||||
| Domain | 614 – 655 | 42 | Helix-loop-helix motif | ||||||
| DNA binding | 601 – 613 | 13 | Basic motif | ||||||
| Region | 656 – 679 | 24 | Class A specific domain | ||||||
| Motif | 181 – 188 | 8 | Nuclear localization signal Potential | ||||||
Amino acid modifications | |||||||||
| Modified residue | 44 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 47 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 305 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 313 | 1 | Phosphothreonine By similarity | ||||||
| Modified residue | 333 | 1 | Phosphoserine By similarity | ||||||
Natural variations | |||||||||
| Alternative sequence | 397 – 420 | 24 | Missing in isoform ALF1A. | VSP_002105 | |||||
Experimental info | |||||||||
| Sequence conflict | 416 | 1 | K → E in M97635. Ref.2 | ||||||
| Sequence conflict | 537 | 1 | V → F in M97635. Ref.2 | ||||||
| Sequence conflict | 537 | 1 | V → F in M97636. Ref.2 | ||||||
Sequences
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References
| [1] | "Murine helix-loop-helix transcriptional activator proteins binding to the E-box motif of the Akv murine leukemia virus enhancer identified by cDNA cloning." Nielsen A.L., Pallisgaard N., Pedersen F.S., Joergensen P. Mol. Cell. Biol. 12:3449-3459(1992) [PubMed: 1321336] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS ALF1A AND ALF1B). |
| [2] | "ME1 and GE1: basic helix-loop-helix transcription factors expressed at high levels in the developing nervous system and in morphogenetically active regions." Neuman T., Keen A., Knapik E., Shain D., Ross M., Nornes H.O., Zuber M.X. Eur. J. Neurosci. 5:311-318(1993) [PubMed: 8261111] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: BALB/c. Tissue: Cerebellum. |
| [3] | "The role of PTF1-P48 in pancreatic acinar gene expression." Rose S.D., Swift G.H., Peyton M.J., Hammer R.E., MacDonald R.J. J. Biol. Chem. 276:44018-44026(2001) [PubMed: 11562365] [Abstract] Cited for: INTERACTION WITH PTF1A. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| X64840 mRNA. Translation: CAA46052.1. M97635 mRNA. No translation available. M97636 mRNA. No translation available. | |
| IPI | IPI00119042. IPI00230668. |
| PIR | C45020. S19958. |
| UniGene | Mm.171615 |
3D structure databases | |
| ModBase | Search... |
PTM databases | |
| PhosphoSite | Q61286. |
Proteomic databases | |
| PRIDE | Q61286. |
Genome annotation databases | |
| Ensembl | ENSMUSG00000032228. Mus musculus. [Contig view] |
Organism-specific databases | |
| MGI | MGI:101877. Tcf12. |
Phylogenomic databases | |
| HOGENOM | Q61286. |
| HOVERGEN | Q61286. |
Gene expression databases | |
| ArrayExpress | Q61286. |
| Bgee | Q61286. |
| CleanEx | MM_TCF12. |
| GermOnline | ENSMUSG00000032228. Mus musculus. |
Family and domain databases | |
| InterPro | IPR001092. HLH_basic. IPR011598. HLH_DNA_bd. [Graphical view] |
| Gene3D | G3DSA:4.10.280.10. HLH_DNA_bd. 1 hit. |
| Pfam | PF00010. HLH. 1 hit. [Graphical view] |
| SMART | SM00353. HLH. 1 hit. [Graphical view] |
| PROSITE | PS50888. HLH. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other Resources | |
| SOURCE | Search... |
Entry information
| Entry name | HTF4_MOUSE | ||||||||
| Accession | Primary (citable) accession number: Q61286 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||
Relevant documents
| MGD cross-references Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot |
| SIMILARITY comments Index of protein domains and families |
