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Q61282

- PGCA_MOUSE

UniProt

Q61282 - PGCA_MOUSE

Protein

Aggrecan core protein

Gene

Acan

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 132 (01 Oct 2014)
      Sequence version 2 (27 Jul 2011)
      Previous versions | rss
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    Functioni

    This proteoglycan is a major component of extracellular matrix of cartilagenous tissues. A major function of this protein is to resist compression in cartilage. It binds avidly to hyaluronic acid via an N-terminal globular region. May play a regulatory role in the matrix assembly of the cartilage.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi1983 – 19831Calcium 1By similarity
    Metal bindingi1987 – 19871Calcium 1By similarity
    Metal bindingi1987 – 19871Calcium 3By similarity
    Metal bindingi2007 – 20071Calcium 2By similarity
    Metal bindingi2009 – 20091Calcium 2By similarity
    Metal bindingi2010 – 20101Calcium 1By similarity
    Metal bindingi2016 – 20161Calcium 1; via carbonyl oxygenBy similarity
    Metal bindingi2016 – 20161Calcium 2By similarity
    Metal bindingi2017 – 20171Calcium 1By similarity
    Metal bindingi2017 – 20171Calcium 3By similarity
    Metal bindingi2030 – 20301Calcium 2By similarity
    Metal bindingi2031 – 20311Calcium 2By similarity
    Metal bindingi2031 – 20311Calcium 2; via carbonyl oxygenBy similarity

    GO - Molecular functioni

    1. carbohydrate binding Source: InterPro
    2. hyaluronic acid binding Source: InterPro
    3. metal ion binding Source: UniProtKB-KW
    4. protein binding Source: UniProtKB

    GO - Biological processi

    1. cartilage condensation Source: MGI
    2. chondrocyte development Source: MGI
    3. collagen fibril organization Source: MGI
    4. proteoglycan biosynthetic process Source: MGI

    Keywords - Ligandi

    Calcium, Lectin, Metal-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Aggrecan core protein
    Alternative name(s):
    Cartilage-specific proteoglycan core protein
    Short name:
    CSPCP
    Gene namesi
    Name:Acan
    Synonyms:Agc, Agc1
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 7

    Organism-specific databases

    MGIiMGI:99602. Acan.

    Subcellular locationi

    GO - Cellular componenti

    1. basement membrane Source: MGI
    2. extracellular matrix Source: MGI
    3. proteinaceous extracellular matrix Source: MGI

    Keywords - Cellular componenti

    Extracellular matrix, Secreted

    Pathology & Biotechi

    Involvement in diseasei

    Defects in Acan are the cause of cartilage matrix deficiency (CMD). CMD is an autosomal recessive syndrome characterized by cleft palate, short limbs, tail and snout. Mutation in strain CMD causes absence of aggrecan by truncation of the protein (mutation in the G1 domain).

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1919Sequence AnalysisAdd
    BLAST
    Chaini20 – 21322113Aggrecan core proteinPRO_0000017507Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi51 ↔ 133By similarity
    Glycosylationi126 – 1261N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi175 ↔ 246By similarity
    Disulfide bondi199 ↔ 220By similarity
    Glycosylationi239 – 2391N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi273 ↔ 348By similarity
    Disulfide bondi297 ↔ 318By similarity
    Glycosylationi333 – 3331N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi371 – 3711O-linked (Xyl...) (keratan sulfate)By similarity
    Glycosylationi376 – 3761O-linked (Xyl...) (keratan sulfate)By similarity
    Glycosylationi387 – 3871N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi509 ↔ 580By similarity
    Disulfide bondi533 ↔ 554By similarity
    Disulfide bondi607 ↔ 682By similarity
    Glycosylationi611 – 6111N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi631 ↔ 652By similarity
    Glycosylationi667 – 6671N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi1675 – 16751N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi1922 ↔ 1933By similarity
    Disulfide bondi1950 ↔ 2042By similarity
    Disulfide bondi2018 ↔ 2034By similarity
    Disulfide bondi2049 ↔ 2092By similarity
    Disulfide bondi2078 ↔ 2105By similarity

    Post-translational modificationi

    Contains mostly chondroitin sulfate, but also keratan sulfate chains, N-linked and O-linked oligosaccharides.

    Keywords - PTMi

    Disulfide bond, Glycoprotein, Proteoglycan

    Proteomic databases

    PaxDbiQ61282.
    PRIDEiQ61282.

    PTM databases

    PhosphoSiteiQ61282.

    Expressioni

    Tissue specificityi

    Specifically expressed in cartilage tissues.1 Publication

    Developmental stagei

    Expressed in chondrocytes throughout the developing skeleton in a pattern very similar but not identical to those of type II and IX collagen. In the newborn mouse skeleton it is expressed essentially in a mutually exclusive manner with tenascin, which is expressed osteoblasts, periosteal and perichondrial cells, and in cells at articular surfaces.1 Publication

    Gene expression databases

    BgeeiQ61282.
    CleanExiMM_ACAN.
    GenevestigatoriQ61282.

    Interactioni

    Subunit structurei

    Interacts with COMP By similarity. Interacts with FBLN1.By similarity1 Publication

    Protein-protein interaction databases

    STRINGi10090.ENSMUSP00000032835.

    Structurei

    3D structure databases

    ProteinModelPortaliQ61282.
    SMRiQ61282. Positions 1920-2045.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini34 – 147114Ig-like V-typeAdd
    BLAST
    Domaini153 – 24896Link 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini254 – 35097Link 2PROSITE-ProRule annotationAdd
    BLAST
    Domaini487 – 58296Link 3PROSITE-ProRule annotationAdd
    BLAST
    Domaini588 – 68497Link 4PROSITE-ProRule annotationAdd
    BLAST
    Domaini1918 – 2044127C-type lectinPROSITE-ProRule annotationAdd
    BLAST
    Domaini2047 – 210761SushiPROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni48 – 14093G1-AAdd
    BLAST
    Regioni152 – 24796G1-BAdd
    BLAST
    Regioni253 – 34997G1-B'Add
    BLAST
    Regioni486 – 58095G2-BAdd
    BLAST
    Regioni587 – 68296G2-B'Add
    BLAST
    Regioni685 – 803119KSAdd
    BLAST
    Regioni805 – 1231427CS-1Add
    BLAST
    Regioni1232 – 1917686CS-2Add
    BLAST
    Regioni1917 – 2132216G3Add
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi1171 – 11733Cell attachment siteSequence Analysis

    Domaini

    Two globular domains, G1 and G2, comprise the N-terminus of the proteoglycan, while another globular region, G3, makes up the C-terminus. G1 contains Link domains and thus consists of three disulfide-bonded loop structures designated as the A, B, B' motifs. G2 is similar to G1. The keratan sulfate (KS) and the chondroitin sulfate (CS) attachment domains lie between G2 and G3.

    Sequence similaritiesi

    Contains 1 C-type lectin domain.PROSITE-ProRule annotation
    Contains 4 Link domains.PROSITE-ProRule annotation
    Contains 1 Sushi (CCP/SCR) domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Immunoglobulin domain, Repeat, Signal, Sushi

    Phylogenomic databases

    eggNOGiNOG12793.
    GeneTreeiENSGT00750000117329.
    HOGENOMiHOG000168421.
    HOVERGENiHBG007982.
    InParanoidiQ61282.
    KOiK06792.
    OMAiCESHNAT.
    OrthoDBiEOG7ZWD1D.
    TreeFamiTF332134.

    Family and domain databases

    Gene3Di2.60.40.10. 1 hit.
    3.10.100.10. 5 hits.
    InterProiIPR001304. C-type_lectin.
    IPR016186. C-type_lectin-like.
    IPR018378. C-type_lectin_CS.
    IPR016187. C-type_lectin_fold.
    IPR007110. Ig-like_dom.
    IPR013783. Ig-like_fold.
    IPR003006. Ig/MHC_CS.
    IPR013106. Ig_V-set.
    IPR003596. Ig_V-set_subgr.
    IPR000538. Link.
    IPR000436. Sushi_SCR_CCP.
    [Graphical view]
    PfamiPF00059. Lectin_C. 1 hit.
    PF00084. Sushi. 1 hit.
    PF07686. V-set. 1 hit.
    PF00193. Xlink. 4 hits.
    [Graphical view]
    PRINTSiPR01265. LINKMODULE.
    SMARTiSM00032. CCP. 1 hit.
    SM00034. CLECT. 1 hit.
    SM00406. IGv. 1 hit.
    SM00445. LINK. 4 hits.
    [Graphical view]
    SUPFAMiSSF56436. SSF56436. 5 hits.
    SSF57535. SSF57535. 1 hit.
    PROSITEiPS00615. C_TYPE_LECTIN_1. 1 hit.
    PS50041. C_TYPE_LECTIN_2. 1 hit.
    PS50835. IG_LIKE. 1 hit.
    PS00290. IG_MHC. 1 hit.
    PS01241. LINK_1. 4 hits.
    PS50963. LINK_2. 4 hits.
    PS50923. SUSHI. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q61282-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTTLLLVFVT LRVIAAVISE EVPDHDNSLS VSIPQPSPLK VLLGSSLTIP     50
    CYFIDPMHPV TTAPSTAPLT PRIKWSRVSK EKEVVLLVAT EGQVRVNSIY 100
    QDKVSLPNYP AIPSDATLEI QNLRSNDSGI YRCEVMHGIE DSEATLEVIV 150
    KGIVFHYRAI STRYTLDFDR AQRACLQNSA IIATPEQLQA AYEDGFHQCD 200
    AGWLADQTVR YPIHTPREGC YGDKDEFPGV RTYGIRDTNE TYDVYCFAEE 250
    MEGEVFYATS PEKFTFQEAA NECRRLGARL ATTGQLYLAW QGGMDMCSAG 300
    WLADRSVRYP ISKARPNCGG NLLGVRTVYL HANQTGYPDP SSRYDAICYT 350
    GEDFVDIPEN FFGVGGEDDI TIQTVTWPDL ELPLPRNVTE GEALGSVILT 400
    AKPIFDLSPT ISEPGEALTL APEVGSTAFP EAEERTGEAT RPWGFPAEVT 450
    RGPDSATAFA SEDLVVRVTI SPGAAEVPGQ PRLPGGVVFH YRPGSTRYSL 500
    TFEEAQQACM HTGAVIASPE QLQAAYEAGY EQCDAGWLQD QTVRYPIVSP 550
    RTPCVGDKDS SPGVRTYGVR PSSETYDVYC YVDKLEGEVF FATRLEQFTF 600
    QEARAFCAAQ NATLASTGQL YAAWSQGLDK CYAGWLADGT LRYPIITPRP 650
    ACGGDKPGVR TVYLYPNQTG LPDPLSKHHA FCFRGVSVAP SPGEEGGSTP 700
    TSPSDIEDWI VTQVGPGVDA VPLEPKTTEV PYFTTEPRKQ TEWEPAYTPV 750
    GTSPQPGIPP TWLPTLPAAE EHTESPSASE EPSASAVPST SEEPYTSSFA 800
    VPSMTELPGS GEASGAPDLS GDFTGSGDAS GRLDSSGQPS GGIESGLPSG 850
    DLDSSGLSPT VSSGLPVESG SASGDGEVPW SHTPTVGRLP SGGESPEGSA 900
    SASGTGDLSG LPSGGEITET STSGAEETSG LPSGGDGLET STSGVDDVSG 950
    IPTGREGLET SASGVEDLSG LPSGEEGSET STSGIEDISV LPTGGESLET 1000
    SASGVGDLSG LPSGGESLET SASGAEDVTQ LPTERGGLET SASGVEDITV 1050
    LPTGRESLET SASGVEDVSG LPSGREGLET SASGIEDISV FPTEAEGLDT 1100
    SASGGYVSGI PSGGDGTETS ASGVEDVSGL PSGGEGLETS ASGVEDLGPS 1150
    TRDSLETSAS GVDVTGFPSG RGDPETSVSG VGDDFSGLPS GKEGLETSAS 1200
    GAEDLSGLPS GKEDLVGSAS GALDFGKLPP GTLGSGQTPE VNGFPSGFSG 1250
    EYSGADIGSG PSSGLPDFSG LPSGFPTVSL VDSTLVEVIT ATTSSELEGR 1300
    GTIGISGSGE VSGLPLGELD SSADISGLPS GTELSGQASG SPDSSGETSG 1350
    FFDVSGQPFG SSGVSEETSG IPEISGQPSG TPDTTATSGV TELNELSSGQ 1400
    PDVSGDGSGI LFGSGQSSGI TSVSGETSGI SDLSGQPSGF PVFSGTATRT 1450
    PDLASGTISG SGESSGITFV DTSFVEVTPT TFREEEGLGS VELSGFPSGE 1500
    TELSGTSGTV DVSEQSSGAI DSSGLTSPTP EFSGLPSGVA EVSGEFSGVE 1550
    TGSSLPSGAF DGSGLVSGFP TVSLVDRTLV ESITQAPTAQ EAGEGPSGIL 1600
    EFSGAHSGTP DISGELSGSL DLSTLQSGQM ETSTETPSSP YFSGDFSSTT 1650
    DVSGESIAAT TGSGESSGLP EVTLNTSELV EGVTEPTVSQ ELGHGPSMTY 1700
    TPRLFEASGD ASASGDLGGA VTNFPGSGIE ASVPEASSDL SAYPEAGVGV 1750
    SAAPEASSKL SEFPDLHGIT SAFHETDLEM TTPSTEVNSN PWTFQEGTRE 1800
    GSAAPEVSGE SSTTSDIDTG TSGVPSATPM ASGDRTEISG EWSDHTSEVN 1850
    VAISSTITES EWAQPTRYPT ETLQEIESPN PSYSGEETQT AETTMSLTDA 1900
    PTLSSSEGSG ETESTVADQE QCEEGWTKFQ GHCYRHFHDR ETWVDAERRC 1950
    REQQSHLSSI VTPEEQEFVN KNAQDYQWIG LNDRTIEGDF RWSDGHSLQF 2000
    EKWRPNQPDN FFATGEDCVV MIWHERGEWN DVPCNYQLPF TCKKGTVACG 2050
    DPPVVEHART LGQKKDRYEI SSLVRYQCTE GFVQRHVPTI RCQPSGHWEE 2100
    PRITCTDPNT YKHRLQKRSM RPTRRSRPSM AH 2132
    Length:2,132
    Mass (Da):221,941
    Last modified:July 27, 2011 - v2
    Checksum:i17298662D95E1921
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti482 – 4821R → H in AAC37670. (PubMed:7806222)Curated
    Sequence conflicti515 – 5151V → I in AAC37670. (PubMed:7806222)Curated
    Sequence conflicti568 – 5681G → R in AAC37670. (PubMed:7806222)Curated
    Sequence conflicti696 – 6961G → E in AAC37670. (PubMed:7806222)Curated
    Sequence conflicti1701 – 17022TP → IS in AAC37670. (PubMed:7806222)Curated
    Sequence conflicti1705 – 17051F → S in AAC37670. (PubMed:7806222)Curated
    Sequence conflicti1729 – 17291I → V in AAC37670. (PubMed:7806222)Curated
    Sequence conflicti1938 – 19381H → P in AAC37670. (PubMed:7806222)Curated
    Sequence conflicti2119 – 21191S → T in AAC37670. (PubMed:7806222)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L07049 mRNA. Translation: AAC37670.1.
    AC110520 Genomic DNA. No translation available.
    S73722, S73721 Genomic DNA. Translation: AAB32160.1.
    CCDSiCCDS21377.1.
    PIRiA55182.
    RefSeqiNP_031450.2. NM_007424.2.
    UniGeneiMm.358571.

    Genome annotation databases

    EnsembliENSMUST00000032835; ENSMUSP00000032835; ENSMUSG00000030607.
    GeneIDi11595.
    KEGGimmu:11595.
    UCSCiuc009hxw.1. mouse.

    Cross-referencesi

    Web resourcesi

    Functional Glycomics Gateway - Glycan Binding

    Aggrecan

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L07049 mRNA. Translation: AAC37670.1 .
    AC110520 Genomic DNA. No translation available.
    S73722 , S73721 Genomic DNA. Translation: AAB32160.1 .
    CCDSi CCDS21377.1.
    PIRi A55182.
    RefSeqi NP_031450.2. NM_007424.2.
    UniGenei Mm.358571.

    3D structure databases

    ProteinModelPortali Q61282.
    SMRi Q61282. Positions 1920-2045.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10090.ENSMUSP00000032835.

    PTM databases

    PhosphoSitei Q61282.

    Proteomic databases

    PaxDbi Q61282.
    PRIDEi Q61282.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000032835 ; ENSMUSP00000032835 ; ENSMUSG00000030607 .
    GeneIDi 11595.
    KEGGi mmu:11595.
    UCSCi uc009hxw.1. mouse.

    Organism-specific databases

    CTDi 176.
    MGIi MGI:99602. Acan.

    Phylogenomic databases

    eggNOGi NOG12793.
    GeneTreei ENSGT00750000117329.
    HOGENOMi HOG000168421.
    HOVERGENi HBG007982.
    InParanoidi Q61282.
    KOi K06792.
    OMAi CESHNAT.
    OrthoDBi EOG7ZWD1D.
    TreeFami TF332134.

    Miscellaneous databases

    NextBioi 279108.
    PROi Q61282.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q61282.
    CleanExi MM_ACAN.
    Genevestigatori Q61282.

    Family and domain databases

    Gene3Di 2.60.40.10. 1 hit.
    3.10.100.10. 5 hits.
    InterProi IPR001304. C-type_lectin.
    IPR016186. C-type_lectin-like.
    IPR018378. C-type_lectin_CS.
    IPR016187. C-type_lectin_fold.
    IPR007110. Ig-like_dom.
    IPR013783. Ig-like_fold.
    IPR003006. Ig/MHC_CS.
    IPR013106. Ig_V-set.
    IPR003596. Ig_V-set_subgr.
    IPR000538. Link.
    IPR000436. Sushi_SCR_CCP.
    [Graphical view ]
    Pfami PF00059. Lectin_C. 1 hit.
    PF00084. Sushi. 1 hit.
    PF07686. V-set. 1 hit.
    PF00193. Xlink. 4 hits.
    [Graphical view ]
    PRINTSi PR01265. LINKMODULE.
    SMARTi SM00032. CCP. 1 hit.
    SM00034. CLECT. 1 hit.
    SM00406. IGv. 1 hit.
    SM00445. LINK. 4 hits.
    [Graphical view ]
    SUPFAMi SSF56436. SSF56436. 5 hits.
    SSF57535. SSF57535. 1 hit.
    PROSITEi PS00615. C_TYPE_LECTIN_1. 1 hit.
    PS50041. C_TYPE_LECTIN_2. 1 hit.
    PS50835. IG_LIKE. 1 hit.
    PS00290. IG_MHC. 1 hit.
    PS01241. LINK_1. 4 hits.
    PS50963. LINK_2. 4 hits.
    PS50923. SUSHI. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Complete coding sequence, deduced primary structure, chromosomal localization, and structural analysis of murine aggrecan."
      Walcz E., Deak F., Erhardt P., Coulter S.N., Fueloep C., Horvath P., Doege K.J., Glant T.T.
      Genomics 22:364-371(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: BALB/c.
      Tissue: Cartilage.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    3. "Mouse cartilage matrix deficiency (cmd) caused by a 7 bp deletion in the aggrecan gene."
      Watanabe H., Kimata K., Line S., Strong D., Gao L.-Y., Kozak C.A., Yamada Y.
      Nat. Genet. 7:154-157(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 211-326.
      Strain: 129/Sv.
    4. "Fibulin-1 is a ligand for the C-type lectin domains of aggrecan and versican."
      Aspberg A., Adam S., Kostka G., Timpl R., Heinegaard D.
      J. Biol. Chem. 274:20444-20449(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH FBLN1.
    5. "Analysis of aggrecan and tenascin gene expression in mouse skeletal tissues by northern and in situ hybridization using species specific cDNA probes."
      Glumoff V., Savontaus M., Vehanen J., Vuorio E.
      Biochim. Biophys. Acta 1219:613-622(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.

    Entry informationi

    Entry nameiPGCA_MOUSE
    AccessioniPrimary (citable) accession number: Q61282
    Secondary accession number(s): E9QLS9, Q64021
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: July 27, 2011
    Last modified: October 1, 2014
    This is version 132 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3