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Q61282

- PGCA_MOUSE

UniProt

Q61282 - PGCA_MOUSE

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Protein
Aggrecan core protein
Gene
Acan, Agc, Agc1
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

This proteoglycan is a major component of extracellular matrix of cartilagenous tissues. A major function of this protein is to resist compression in cartilage. It binds avidly to hyaluronic acid via an N-terminal globular region. May play a regulatory role in the matrix assembly of the cartilage.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi1983 – 19831Calcium 1 By similarity
Metal bindingi1987 – 19871Calcium 1 By similarity
Metal bindingi1987 – 19871Calcium 3 By similarity
Metal bindingi2007 – 20071Calcium 2 By similarity
Metal bindingi2009 – 20091Calcium 2 By similarity
Metal bindingi2010 – 20101Calcium 1 By similarity
Metal bindingi2016 – 20161Calcium 1; via carbonyl oxygen By similarity
Metal bindingi2016 – 20161Calcium 2 By similarity
Metal bindingi2017 – 20171Calcium 1 By similarity
Metal bindingi2017 – 20171Calcium 3 By similarity
Metal bindingi2030 – 20301Calcium 2 By similarity
Metal bindingi2031 – 20311Calcium 2 By similarity
Metal bindingi2031 – 20311Calcium 2; via carbonyl oxygen By similarity

GO - Molecular functioni

  1. carbohydrate binding Source: InterPro
  2. hyaluronic acid binding Source: InterPro
  3. metal ion binding Source: UniProtKB-KW
  4. protein binding Source: UniProtKB

GO - Biological processi

  1. cartilage condensation Source: MGI
  2. chondrocyte development Source: MGI
  3. collagen fibril organization Source: MGI
  4. proteoglycan biosynthetic process Source: MGI
Complete GO annotation...

Keywords - Ligandi

Calcium, Lectin, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Aggrecan core protein
Alternative name(s):
Cartilage-specific proteoglycan core protein
Short name:
CSPCP
Gene namesi
Name:Acan
Synonyms:Agc, Agc1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 7

Organism-specific databases

MGIiMGI:99602. Acan.

Subcellular locationi

GO - Cellular componenti

  1. basement membrane Source: MGI
  2. extracellular matrix Source: MGI
  3. proteinaceous extracellular matrix Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Extracellular matrix, Secreted

Pathology & Biotechi

Involvement in diseasei

Defects in Acan are the cause of cartilage matrix deficiency (CMD). CMD is an autosomal recessive syndrome characterized by cleft palate, short limbs, tail and snout. Mutation in strain CMD causes absence of aggrecan by truncation of the protein (mutation in the G1 domain).

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1919 Reviewed prediction
Add
BLAST
Chaini20 – 21322113Aggrecan core protein
PRO_0000017507Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi51 ↔ 133 By similarity
Glycosylationi126 – 1261N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi175 ↔ 246 By similarity
Disulfide bondi199 ↔ 220 By similarity
Glycosylationi239 – 2391N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi273 ↔ 348 By similarity
Disulfide bondi297 ↔ 318 By similarity
Glycosylationi333 – 3331N-linked (GlcNAc...) Reviewed prediction
Glycosylationi371 – 3711O-linked (Xyl...) (keratan sulfate) By similarity
Glycosylationi376 – 3761O-linked (Xyl...) (keratan sulfate) By similarity
Glycosylationi387 – 3871N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi509 ↔ 580 By similarity
Disulfide bondi533 ↔ 554 By similarity
Disulfide bondi607 ↔ 682 By similarity
Glycosylationi611 – 6111N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi631 ↔ 652 By similarity
Glycosylationi667 – 6671N-linked (GlcNAc...) Reviewed prediction
Glycosylationi1675 – 16751N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi1922 ↔ 1933 By similarity
Disulfide bondi1950 ↔ 2042 By similarity
Disulfide bondi2018 ↔ 2034 By similarity
Disulfide bondi2049 ↔ 2092 By similarity
Disulfide bondi2078 ↔ 2105 By similarity

Post-translational modificationi

Contains mostly chondroitin sulfate, but also keratan sulfate chains, N-linked and O-linked oligosaccharides.

Keywords - PTMi

Disulfide bond, Glycoprotein, Proteoglycan

Proteomic databases

PaxDbiQ61282.
PRIDEiQ61282.

PTM databases

PhosphoSiteiQ61282.

Expressioni

Tissue specificityi

Specifically expressed in cartilage tissues.1 Publication

Developmental stagei

Expressed in chondrocytes throughout the developing skeleton in a pattern very similar but not identical to those of type II and IX collagen. In the newborn mouse skeleton it is expressed essentially in a mutually exclusive manner with tenascin, which is expressed osteoblasts, periosteal and perichondrial cells, and in cells at articular surfaces.1 Publication

Gene expression databases

BgeeiQ61282.
CleanExiMM_ACAN.
GenevestigatoriQ61282.

Interactioni

Subunit structurei

Interacts with COMP By similarity. Interacts with FBLN1.1 Publication

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000032835.

Structurei

3D structure databases

ProteinModelPortaliQ61282.
SMRiQ61282. Positions 1920-2045.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini34 – 147114Ig-like V-type
Add
BLAST
Domaini153 – 24896Link 1
Add
BLAST
Domaini254 – 35097Link 2
Add
BLAST
Domaini487 – 58296Link 3
Add
BLAST
Domaini588 – 68497Link 4
Add
BLAST
Domaini1918 – 2044127C-type lectin
Add
BLAST
Domaini2047 – 210761Sushi
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni48 – 14093G1-A
Add
BLAST
Regioni152 – 24796G1-B
Add
BLAST
Regioni253 – 34997G1-B'
Add
BLAST
Regioni486 – 58095G2-B
Add
BLAST
Regioni587 – 68296G2-B'
Add
BLAST
Regioni685 – 803119KS
Add
BLAST
Regioni805 – 1231427CS-1
Add
BLAST
Regioni1232 – 1917686CS-2
Add
BLAST
Regioni1917 – 2132216G3
Add
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi1171 – 11733Cell attachment site Reviewed prediction

Domaini

Two globular domains, G1 and G2, comprise the N-terminus of the proteoglycan, while another globular region, G3, makes up the C-terminus. G1 contains Link domains and thus consists of three disulfide-bonded loop structures designated as the A, B, B' motifs. G2 is similar to G1. The keratan sulfate (KS) and the chondroitin sulfate (CS) attachment domains lie between G2 and G3.

Sequence similaritiesi

Contains 4 Link domains.

Keywords - Domaini

Immunoglobulin domain, Repeat, Signal, Sushi

Phylogenomic databases

eggNOGiNOG12793.
GeneTreeiENSGT00750000117329.
HOGENOMiHOG000168421.
HOVERGENiHBG007982.
InParanoidiQ61282.
KOiK06792.
OMAiCESHNAT.
OrthoDBiEOG7ZWD1D.
TreeFamiTF332134.

Family and domain databases

Gene3Di2.60.40.10. 1 hit.
3.10.100.10. 5 hits.
InterProiIPR001304. C-type_lectin.
IPR016186. C-type_lectin-like.
IPR018378. C-type_lectin_CS.
IPR016187. C-type_lectin_fold.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003006. Ig/MHC_CS.
IPR013106. Ig_V-set.
IPR003596. Ig_V-set_subgr.
IPR000538. Link.
IPR000436. Sushi_SCR_CCP.
[Graphical view]
PfamiPF00059. Lectin_C. 1 hit.
PF00084. Sushi. 1 hit.
PF07686. V-set. 1 hit.
PF00193. Xlink. 4 hits.
[Graphical view]
PRINTSiPR01265. LINKMODULE.
SMARTiSM00032. CCP. 1 hit.
SM00034. CLECT. 1 hit.
SM00406. IGv. 1 hit.
SM00445. LINK. 4 hits.
[Graphical view]
SUPFAMiSSF56436. SSF56436. 5 hits.
SSF57535. SSF57535. 1 hit.
PROSITEiPS00615. C_TYPE_LECTIN_1. 1 hit.
PS50041. C_TYPE_LECTIN_2. 1 hit.
PS50835. IG_LIKE. 1 hit.
PS00290. IG_MHC. 1 hit.
PS01241. LINK_1. 4 hits.
PS50963. LINK_2. 4 hits.
PS50923. SUSHI. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q61282-1 [UniParc]FASTAAdd to Basket

« Hide

MTTLLLVFVT LRVIAAVISE EVPDHDNSLS VSIPQPSPLK VLLGSSLTIP     50
CYFIDPMHPV TTAPSTAPLT PRIKWSRVSK EKEVVLLVAT EGQVRVNSIY 100
QDKVSLPNYP AIPSDATLEI QNLRSNDSGI YRCEVMHGIE DSEATLEVIV 150
KGIVFHYRAI STRYTLDFDR AQRACLQNSA IIATPEQLQA AYEDGFHQCD 200
AGWLADQTVR YPIHTPREGC YGDKDEFPGV RTYGIRDTNE TYDVYCFAEE 250
MEGEVFYATS PEKFTFQEAA NECRRLGARL ATTGQLYLAW QGGMDMCSAG 300
WLADRSVRYP ISKARPNCGG NLLGVRTVYL HANQTGYPDP SSRYDAICYT 350
GEDFVDIPEN FFGVGGEDDI TIQTVTWPDL ELPLPRNVTE GEALGSVILT 400
AKPIFDLSPT ISEPGEALTL APEVGSTAFP EAEERTGEAT RPWGFPAEVT 450
RGPDSATAFA SEDLVVRVTI SPGAAEVPGQ PRLPGGVVFH YRPGSTRYSL 500
TFEEAQQACM HTGAVIASPE QLQAAYEAGY EQCDAGWLQD QTVRYPIVSP 550
RTPCVGDKDS SPGVRTYGVR PSSETYDVYC YVDKLEGEVF FATRLEQFTF 600
QEARAFCAAQ NATLASTGQL YAAWSQGLDK CYAGWLADGT LRYPIITPRP 650
ACGGDKPGVR TVYLYPNQTG LPDPLSKHHA FCFRGVSVAP SPGEEGGSTP 700
TSPSDIEDWI VTQVGPGVDA VPLEPKTTEV PYFTTEPRKQ TEWEPAYTPV 750
GTSPQPGIPP TWLPTLPAAE EHTESPSASE EPSASAVPST SEEPYTSSFA 800
VPSMTELPGS GEASGAPDLS GDFTGSGDAS GRLDSSGQPS GGIESGLPSG 850
DLDSSGLSPT VSSGLPVESG SASGDGEVPW SHTPTVGRLP SGGESPEGSA 900
SASGTGDLSG LPSGGEITET STSGAEETSG LPSGGDGLET STSGVDDVSG 950
IPTGREGLET SASGVEDLSG LPSGEEGSET STSGIEDISV LPTGGESLET 1000
SASGVGDLSG LPSGGESLET SASGAEDVTQ LPTERGGLET SASGVEDITV 1050
LPTGRESLET SASGVEDVSG LPSGREGLET SASGIEDISV FPTEAEGLDT 1100
SASGGYVSGI PSGGDGTETS ASGVEDVSGL PSGGEGLETS ASGVEDLGPS 1150
TRDSLETSAS GVDVTGFPSG RGDPETSVSG VGDDFSGLPS GKEGLETSAS 1200
GAEDLSGLPS GKEDLVGSAS GALDFGKLPP GTLGSGQTPE VNGFPSGFSG 1250
EYSGADIGSG PSSGLPDFSG LPSGFPTVSL VDSTLVEVIT ATTSSELEGR 1300
GTIGISGSGE VSGLPLGELD SSADISGLPS GTELSGQASG SPDSSGETSG 1350
FFDVSGQPFG SSGVSEETSG IPEISGQPSG TPDTTATSGV TELNELSSGQ 1400
PDVSGDGSGI LFGSGQSSGI TSVSGETSGI SDLSGQPSGF PVFSGTATRT 1450
PDLASGTISG SGESSGITFV DTSFVEVTPT TFREEEGLGS VELSGFPSGE 1500
TELSGTSGTV DVSEQSSGAI DSSGLTSPTP EFSGLPSGVA EVSGEFSGVE 1550
TGSSLPSGAF DGSGLVSGFP TVSLVDRTLV ESITQAPTAQ EAGEGPSGIL 1600
EFSGAHSGTP DISGELSGSL DLSTLQSGQM ETSTETPSSP YFSGDFSSTT 1650
DVSGESIAAT TGSGESSGLP EVTLNTSELV EGVTEPTVSQ ELGHGPSMTY 1700
TPRLFEASGD ASASGDLGGA VTNFPGSGIE ASVPEASSDL SAYPEAGVGV 1750
SAAPEASSKL SEFPDLHGIT SAFHETDLEM TTPSTEVNSN PWTFQEGTRE 1800
GSAAPEVSGE SSTTSDIDTG TSGVPSATPM ASGDRTEISG EWSDHTSEVN 1850
VAISSTITES EWAQPTRYPT ETLQEIESPN PSYSGEETQT AETTMSLTDA 1900
PTLSSSEGSG ETESTVADQE QCEEGWTKFQ GHCYRHFHDR ETWVDAERRC 1950
REQQSHLSSI VTPEEQEFVN KNAQDYQWIG LNDRTIEGDF RWSDGHSLQF 2000
EKWRPNQPDN FFATGEDCVV MIWHERGEWN DVPCNYQLPF TCKKGTVACG 2050
DPPVVEHART LGQKKDRYEI SSLVRYQCTE GFVQRHVPTI RCQPSGHWEE 2100
PRITCTDPNT YKHRLQKRSM RPTRRSRPSM AH 2132
Length:2,132
Mass (Da):221,941
Last modified:July 27, 2011 - v2
Checksum:i17298662D95E1921
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti482 – 4821R → H in AAC37670. 1 Publication
Sequence conflicti515 – 5151V → I in AAC37670. 1 Publication
Sequence conflicti568 – 5681G → R in AAC37670. 1 Publication
Sequence conflicti696 – 6961G → E in AAC37670. 1 Publication
Sequence conflicti1701 – 17022TP → IS in AAC37670. 1 Publication
Sequence conflicti1705 – 17051F → S in AAC37670. 1 Publication
Sequence conflicti1729 – 17291I → V in AAC37670. 1 Publication
Sequence conflicti1938 – 19381H → P in AAC37670. 1 Publication
Sequence conflicti2119 – 21191S → T in AAC37670. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L07049 mRNA. Translation: AAC37670.1.
AC110520 Genomic DNA. No translation available.
S73722, S73721 Genomic DNA. Translation: AAB32160.1.
CCDSiCCDS21377.1.
PIRiA55182.
RefSeqiNP_031450.2. NM_007424.2.
UniGeneiMm.358571.

Genome annotation databases

EnsembliENSMUST00000032835; ENSMUSP00000032835; ENSMUSG00000030607.
GeneIDi11595.
KEGGimmu:11595.
UCSCiuc009hxw.1. mouse.

Cross-referencesi

Web resourcesi

Functional Glycomics Gateway - Glycan Binding

Aggrecan

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L07049 mRNA. Translation: AAC37670.1 .
AC110520 Genomic DNA. No translation available.
S73722 , S73721 Genomic DNA. Translation: AAB32160.1 .
CCDSi CCDS21377.1.
PIRi A55182.
RefSeqi NP_031450.2. NM_007424.2.
UniGenei Mm.358571.

3D structure databases

ProteinModelPortali Q61282.
SMRi Q61282. Positions 1920-2045.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 10090.ENSMUSP00000032835.

PTM databases

PhosphoSitei Q61282.

Proteomic databases

PaxDbi Q61282.
PRIDEi Q61282.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000032835 ; ENSMUSP00000032835 ; ENSMUSG00000030607 .
GeneIDi 11595.
KEGGi mmu:11595.
UCSCi uc009hxw.1. mouse.

Organism-specific databases

CTDi 176.
MGIi MGI:99602. Acan.

Phylogenomic databases

eggNOGi NOG12793.
GeneTreei ENSGT00750000117329.
HOGENOMi HOG000168421.
HOVERGENi HBG007982.
InParanoidi Q61282.
KOi K06792.
OMAi CESHNAT.
OrthoDBi EOG7ZWD1D.
TreeFami TF332134.

Miscellaneous databases

NextBioi 279108.
PROi Q61282.
SOURCEi Search...

Gene expression databases

Bgeei Q61282.
CleanExi MM_ACAN.
Genevestigatori Q61282.

Family and domain databases

Gene3Di 2.60.40.10. 1 hit.
3.10.100.10. 5 hits.
InterProi IPR001304. C-type_lectin.
IPR016186. C-type_lectin-like.
IPR018378. C-type_lectin_CS.
IPR016187. C-type_lectin_fold.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003006. Ig/MHC_CS.
IPR013106. Ig_V-set.
IPR003596. Ig_V-set_subgr.
IPR000538. Link.
IPR000436. Sushi_SCR_CCP.
[Graphical view ]
Pfami PF00059. Lectin_C. 1 hit.
PF00084. Sushi. 1 hit.
PF07686. V-set. 1 hit.
PF00193. Xlink. 4 hits.
[Graphical view ]
PRINTSi PR01265. LINKMODULE.
SMARTi SM00032. CCP. 1 hit.
SM00034. CLECT. 1 hit.
SM00406. IGv. 1 hit.
SM00445. LINK. 4 hits.
[Graphical view ]
SUPFAMi SSF56436. SSF56436. 5 hits.
SSF57535. SSF57535. 1 hit.
PROSITEi PS00615. C_TYPE_LECTIN_1. 1 hit.
PS50041. C_TYPE_LECTIN_2. 1 hit.
PS50835. IG_LIKE. 1 hit.
PS00290. IG_MHC. 1 hit.
PS01241. LINK_1. 4 hits.
PS50963. LINK_2. 4 hits.
PS50923. SUSHI. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Complete coding sequence, deduced primary structure, chromosomal localization, and structural analysis of murine aggrecan."
    Walcz E., Deak F., Erhardt P., Coulter S.N., Fueloep C., Horvath P., Doege K.J., Glant T.T.
    Genomics 22:364-371(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: BALB/c.
    Tissue: Cartilage.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. "Mouse cartilage matrix deficiency (cmd) caused by a 7 bp deletion in the aggrecan gene."
    Watanabe H., Kimata K., Line S., Strong D., Gao L.-Y., Kozak C.A., Yamada Y.
    Nat. Genet. 7:154-157(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 211-326.
    Strain: 129/Sv.
  4. "Fibulin-1 is a ligand for the C-type lectin domains of aggrecan and versican."
    Aspberg A., Adam S., Kostka G., Timpl R., Heinegaard D.
    J. Biol. Chem. 274:20444-20449(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH FBLN1.
  5. "Analysis of aggrecan and tenascin gene expression in mouse skeletal tissues by northern and in situ hybridization using species specific cDNA probes."
    Glumoff V., Savontaus M., Vehanen J., Vuorio E.
    Biochim. Biophys. Acta 1219:613-622(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.

Entry informationi

Entry nameiPGCA_MOUSE
AccessioniPrimary (citable) accession number: Q61282
Secondary accession number(s): E9QLS9, Q64021
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: July 27, 2011
Last modified: July 9, 2014
This is version 131 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi