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Protein

Sterol O-acyltransferase 1

Gene

Soat1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the formation of fatty acid-cholesterol esters, which are less soluble in membranes than cholesterol. Plays a role in lipoprotein assembly and dietary cholesterol absorption.

Catalytic activityi

Acyl-CoA + cholesterol = CoA + cholesterol ester.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei450 – 4501By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Keywords - Biological processi

Cholesterol metabolism, Lipid metabolism, Steroid metabolism, Sterol metabolism

Enzyme and pathway databases

BRENDAi2.3.1.26. 3474.

Names & Taxonomyi

Protein namesi
Recommended name:
Sterol O-acyltransferase 1 (EC:2.3.1.26By similarity)
Alternative name(s):
Acyl-coenzyme A:cholesterol acyltransferase 1
Short name:
ACAT-1
Cholesterol acyltransferase 1
Gene namesi
Name:Soat1
Synonyms:Acact
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:104665. Soat1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 140140CytoplasmicSequence analysisAdd
BLAST
Transmembranei141 – 15919HelicalSequence analysisAdd
BLAST
Topological domaini160 – 18122LumenalSequence analysisAdd
BLAST
Transmembranei182 – 19918HelicalSequence analysisAdd
BLAST
Topological domaini200 – 22425CytoplasmicSequence analysisAdd
BLAST
Transmembranei225 – 24319HelicalSequence analysisAdd
BLAST
Topological domaini244 – 2463LumenalSequence analysis
Transmembranei247 – 26418HelicalSequence analysisAdd
BLAST
Topological domaini265 – 501237CytoplasmicSequence analysisAdd
BLAST
Transmembranei502 – 51716HelicalSequence analysisAdd
BLAST
Topological domaini518 – 54023LumenalSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

Pathology & Biotechi

Chemistry

ChEMBLiCHEMBL4464.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 540540Sterol O-acyltransferase 1PRO_0000207642Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21PhosphoserineBy similarity
Disulfide bondi518 ↔ 536By similarity

Keywords - PTMi

Disulfide bond, Phosphoprotein

Proteomic databases

EPDiQ61263.
MaxQBiQ61263.
PaxDbiQ61263.
PRIDEiQ61263.

PTM databases

iPTMnetiQ61263.
PhosphoSiteiQ61263.
SwissPalmiQ61263.

Expressioni

Gene expression databases

BgeeiENSMUSG00000026600.
CleanExiMM_SOAT1.
ExpressionAtlasiQ61263. baseline and differential.
GenevisibleiQ61263. MM.

Interactioni

Subunit structurei

May form homo- or heterodimers. Interacts with UBIAD1.By similarity

Protein-protein interaction databases

BioGridi203385. 1 interaction.
IntActiQ61263. 1 interaction.
MINTiMINT-4135192.
STRINGi10090.ENSMUSP00000058344.

Chemistry

BindingDBiQ61263.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0380. Eukaryota.
COG5056. LUCA.
GeneTreeiENSGT00530000063122.
HOGENOMiHOG000020782.
HOVERGENiHBG058198.
InParanoidiQ61263.
KOiK00637.
OMAiWLNFTSE.
OrthoDBiEOG091G0991.
TreeFamiTF105767.

Family and domain databases

InterProiIPR004299. MBOAT_fam.
IPR014371. Oat_ACAT_DAG_ARE.
IPR030687. Sterol_acyltranf_meta.
[Graphical view]
PANTHERiPTHR10408. PTHR10408. 1 hit.
PfamiPF03062. MBOAT. 1 hit.
[Graphical view]
PIRSFiPIRSF000439. Oat_ACAT_DAG_ARE. 1 hit.
PIRSF500230. Sterol_acyltranf_ACAT. 1 hit.

Sequencei

Sequence statusi: Complete.

Q61263-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSLRNRLSKS GENPEQDEAQ KNFMDTYRNG HITMKQLIAK KRLLAAEAEE
60 70 80 90 100
LKPLFMKEVG CHFDDFVTNL IEKSASLDNG GCALTTFSIL EEMKKNHRAK
110 120 130 140 150
DLRAPPEQGK IFISRQSLLD ELFEVDHIRT IYHMFIALLI LFVLSTIVVD
160 170 180 190 200
YIDEGRLVLE FNLLAYAFGK FPTVIWTWWA MFLSTLSIPY FLFQRWAHGY
210 220 230 240 250
SKSSHPLIYS LVHGLLFLVF QLGVLGFVPT YVVLAYTLPP ASRFILILEQ
260 270 280 290 300
IRLIMKAHSF VRENIPRVLN AAKEKSSKDP LPTVNQYLYF LFAPTLIYRD
310 320 330 340 350
NYPRTPTVRW GYVAMQFLQV FGCLFYVYYI FERLCAPLFR NIKQEPFSAR
360 370 380 390 400
VLVLCVFNSI LPGVLILFLS FFAFLHCWLN AFAEMLRFGD RMFYKDWWNS
410 420 430 440 450
TSYSNYYRTW NVVVHDWLYY YVYKDLLWFF SKRFKSAAML AVFALSAVVH
460 470 480 490 500
EYALAICLSY FYPVLFVLFM FFGMAFNFIV NDSRKRPIWN IMVWASLFLG
510 520 530 540
YGLILCFYSQ EWYARQHCPL KNPTFLDYVR PRTWTCRYVF
Length:540
Mass (Da):63,799
Last modified:July 27, 2011 - v2
Checksum:iF2A6C91774762E23
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti195 – 1951R → P in AAC42075 (PubMed:7592824).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L42293 mRNA. Translation: AAC42075.1.
S81092 mRNA. Translation: AAB36050.1.
AK142486 mRNA. Translation: BAE25081.1.
AK159393 mRNA. Translation: BAE35047.1.
AK159529 mRNA. Translation: BAE35158.1.
AK160017 mRNA. Translation: BAE35563.1.
BC083092 mRNA. Translation: AAH83092.1.
CCDSiCCDS35743.1.
PIRiI49454.
RefSeqiNP_033256.2. NM_009230.3.
UniGeneiMm.28099.

Genome annotation databases

EnsembliENSMUST00000051396; ENSMUSP00000058344; ENSMUSG00000026600.
ENSMUST00000189661; ENSMUSP00000140721; ENSMUSG00000026600.
GeneIDi20652.
KEGGimmu:20652.
UCSCiuc007dcj.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L42293 mRNA. Translation: AAC42075.1.
S81092 mRNA. Translation: AAB36050.1.
AK142486 mRNA. Translation: BAE25081.1.
AK159393 mRNA. Translation: BAE35047.1.
AK159529 mRNA. Translation: BAE35158.1.
AK160017 mRNA. Translation: BAE35563.1.
BC083092 mRNA. Translation: AAH83092.1.
CCDSiCCDS35743.1.
PIRiI49454.
RefSeqiNP_033256.2. NM_009230.3.
UniGeneiMm.28099.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi203385. 1 interaction.
IntActiQ61263. 1 interaction.
MINTiMINT-4135192.
STRINGi10090.ENSMUSP00000058344.

Chemistry

BindingDBiQ61263.
ChEMBLiCHEMBL4464.

PTM databases

iPTMnetiQ61263.
PhosphoSiteiQ61263.
SwissPalmiQ61263.

Proteomic databases

EPDiQ61263.
MaxQBiQ61263.
PaxDbiQ61263.
PRIDEiQ61263.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000051396; ENSMUSP00000058344; ENSMUSG00000026600.
ENSMUST00000189661; ENSMUSP00000140721; ENSMUSG00000026600.
GeneIDi20652.
KEGGimmu:20652.
UCSCiuc007dcj.1. mouse.

Organism-specific databases

CTDi6646.
MGIiMGI:104665. Soat1.

Phylogenomic databases

eggNOGiKOG0380. Eukaryota.
COG5056. LUCA.
GeneTreeiENSGT00530000063122.
HOGENOMiHOG000020782.
HOVERGENiHBG058198.
InParanoidiQ61263.
KOiK00637.
OMAiWLNFTSE.
OrthoDBiEOG091G0991.
TreeFamiTF105767.

Enzyme and pathway databases

BRENDAi2.3.1.26. 3474.

Miscellaneous databases

ChiTaRSiSoat1. mouse.
PROiQ61263.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000026600.
CleanExiMM_SOAT1.
ExpressionAtlasiQ61263. baseline and differential.
GenevisibleiQ61263. MM.

Family and domain databases

InterProiIPR004299. MBOAT_fam.
IPR014371. Oat_ACAT_DAG_ARE.
IPR030687. Sterol_acyltranf_meta.
[Graphical view]
PANTHERiPTHR10408. PTHR10408. 1 hit.
PfamiPF03062. MBOAT. 1 hit.
[Graphical view]
PIRSFiPIRSF000439. Oat_ACAT_DAG_ARE. 1 hit.
PIRSF500230. Sterol_acyltranf_ACAT. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiSOAT1_MOUSE
AccessioniPrimary (citable) accession number: Q61263
Secondary accession number(s): Q5XK32, Q64180
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 18, 2001
Last sequence update: July 27, 2011
Last modified: September 7, 2016
This is version 124 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.