Reviewed, UniProtKB/Swiss-Prot Q61263 (SOAT1_MOUSE)

Names and origin

Protein names

Recommended name:
    Sterol O-acyltransferase 1
    EC=2.3.1.26
Alternative name(s):
    Cholesterol acyltransferase 1
    Acyl coenzyme A:cholesterol acyltransferase 1
      Short name=ACAT-1

Gene names

Name:	Soat1
Synonyms:	Acact

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	540 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the formation of fatty acid-cholesterol esters. Plays a role in lipoprotein assembly and dietary cholesterol absorption.
Catalytic activity	Acyl-CoA + cholesterol = CoA + cholesterol ester.
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the membrane-bound acyltransferase family. Sterol o-acyltransferase subfamily.

Ontologies

Keywords
Biological process	Cholesterol metabolism Lipid metabolism Steroid metabolism
Cellular component	Endoplasmic reticulum Membrane
Domain	Transmembrane
Molecular function	Acyltransferase Transferase
Gene Ontology (GO)
Biological process	cholesterol metabolic process Inferred from direct assay. Source: MGI
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell microsome Inferred from direct assay. Source: MGI
Molecular function	cholesterol O-acyltransferase activity Inferred from electronic annotation. Source: EC ergosterol O-acyltransferase activity Inferred from electronic annotation. Source: EC lanosterol O-acyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 540	540	Sterol O-acyltransferase 1		PRO_0000207642
Regions
	Transmembrane	132 – 152	21	Potential
	Transmembrane	311 – 331	21	Potential
	Transmembrane	352 – 372	21	Potential
	Transmembrane	460 – 480	21	Potential
	Transmembrane	488 – 508	21	Potential
Sites
	Active site	450	1	Potential
Experimental info
	Sequence conflict	195	1	P → R in AAB36050. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q61263-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 8EF900C8BCDF73C0	FASTA	540	63,740
	10 20 30 40 50 60 MSLRNRLSKS GENPEQDEAQ KNFMDTYRNG HITMKQLIAK KRLLAAEAEE LKPLFMKEVG 70 80 90 100 110 120 CHFDDFVTNL IEKSASLDNG GCALTTFSIL EEMKKNHRAK DLRAPPEQGK IFISRQSLLD 130 140 150 160 170 180 ELFEVDHIRT IYHMFIALLI LFVLSTIVVD YIDEGRLVLE FNLLAYAFGK FPTVIWTWWA 190 200 210 220 230 240 MFLSTLSIPY FLFQPWAHGY SKSSHPLIYS LVHGLLFLVF QLGVLGFVPT YVVLAYTLPP 250 260 270 280 290 300 ASRFILILEQ IRLIMKAHSF VRENIPRVLN AAKEKSSKDP LPTVNQYLYF LFAPTLIYRD 310 320 330 340 350 360 NYPRTPTVRW GYVAMQFLQV FGCLFYVYYI FERLCAPLFR NIKQEPFSAR VLVLCVFNSI 370 380 390 400 410 420 LPGVLILFLS FFAFLHCWLN AFAEMLRFGD RMFYKDWWNS TSYSNYYRTW NVVVHDWLYY 430 440 450 460 470 480 YVYKDLLWFF SKRFKSAAML AVFALSAVVH EYALAICLSY FYPVLFVLFM FFGMAFNFIV 490 500 510 520 530 540 NDSRKRPIWN IMVWASLFLG YGLILCFYSQ EWYARQHCPL KNPTFLDYVR PRTWTCRYVF

References

[1]	"Tissue-specific expression and cholesterol regulation of acylcoenzyme A:cholesterol acyltransferase (ACAT) in mice. Molecular cloning of mouse ACAT cDNA, chromosomal localization, and regulation of ACAT in vivo and in vitro." Uelmen P.J., Oka K., Sullivan M.C., Chang T.-Y., Chang C.C.Y., Chan L. J. Biol. Chem. 270:26192-26201(1995) [PubMed: 7592824] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Liver.
[2]	"Cloning and expression in Xenopus oocytes of a mouse homologue of the human acylcoenzyme A: cholesterol acyltransferase and its potential role in metabolism of oxidized LDL." Green S., Steinberg D., Quehenberger O. Biochem. Biophys. Res. Commun. 218:924-929(1996) [PubMed: 8579615] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L42293 mRNA. Translation: AAC42075.1. S81092 mRNA. Translation: AAB36050.1.
IPI	IPI00278153.
PIR	I49454.
UniGene	Mm.28099
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q61263.
Proteomic databases
PRIDE	Q61263.
Genome annotation databases
Ensembl	ENSMUST00000051396; ENSMUSP00000058344; ENSMUSG00000026600; Mus musculus. [Genome view]
Organism-specific databases
MGI	MGI:104665. Soat1.
Phylogenomic databases
HOGENOM	HBG446698.
HOVERGEN	Q61263.
InParanoid	Q61263.
Enzyme and pathway databases
BRENDA	2.3.1.26. 244.
Gene expression databases
ArrayExpress	Q61263.
Bgee	Q61263.
CleanEx	MM_SOAT1.
Genevestigator	Q61263.
GermOnline	ENSMUSG00000026600. Mus musculus.
Family and domain databases
InterPro	IPR004299. MBOAT_fam. [Graphical view]
Pfam	PF03062. MBOAT. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
SOURCE	Search...

Entry information

Entry name

SOAT1_MOUSE

Accession

Primary (citable) accession number: Q61263
Secondary accession number(s): Q64180

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 18, 2001
Last sequence update:	November 1, 1996
Last modified:	December 15, 2009
This is version 72 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)