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Protein

Immunoglobulin-binding protein 1

Gene

Igbp1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Associated to surface IgM-receptor; may be involved in signal transduction. Involved in regulation of the catalytic activity of the phosphatases PP2A, PP4 and PP6 by protecting their partially folded catalytic subunits from degradative polyubiquitination until they associate with regulatory subunits.

GO - Molecular functioni

  • mitogen-activated protein kinase kinase binding Source: UniProtKB
  • protein domain specific binding Source: UniProtKB
  • protein phosphatase 2A binding Source: GO_Central
  • protein phosphatase type 2A regulator activity Source: MGI

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Chaperone

Keywords - Biological processi

B-cell activation

Names & Taxonomyi

Protein namesi
Recommended name:
Immunoglobulin-binding protein 1
Alternative name(s):
Alpha4 phosphoprotein
CD79a-binding protein 1
Lymphocyte signal transduction molecule alpha 4
Protein phosphatase 2/4/6 regulatory subunit
p52
Gene namesi
Name:Igbp1
Synonyms:Pc52
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome X

Organism-specific databases

MGIiMGI:1346500. Igbp1.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB
  • cytosol Source: GO_Central
  • microtubule Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 340340Immunoglobulin-binding protein 1PRO_0000218619Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei242 – 2421N6-acetyllysineBy similarity

Post-translational modificationi

Phosphorylated.
Monoubiquitination by MID1 triggers calpain-mediated cleavage and switches IGBP1 activity from protective to destructive.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei256 – 2572Cleavage; by calpainBy similarity

Keywords - PTMi

Acetylation, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ61249.
MaxQBiQ61249.
PaxDbiQ61249.
PeptideAtlasiQ61249.
PRIDEiQ61249.

2D gel databases

REPRODUCTION-2DPAGEQ61249.

PTM databases

iPTMnetiQ61249.
PhosphoSiteiQ61249.

Expressioni

Tissue specificityi

Expressed in spleen, thymus, liver and brain. Ubiquitously expressed in B lineage cell lines.

Gene expression databases

BgeeiQ61249.
CleanExiMM_IGBP1.
GenevisibleiQ61249. MM.

Interactioni

Subunit structurei

Interacts with PPP2CB, and with PP4 and PP6. Interacts with MID2. Interacts with ubiquitin (By similarity). Interacts with partially folded PPP2CA, but not with the fully active protein. Interacts with MID1.By similarity1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
PPP2CAP677753EBI-7002233,EBI-712311From a different organism.
Ppp2caP633302EBI-7002233,EBI-397144

GO - Molecular functioni

  • mitogen-activated protein kinase kinase binding Source: UniProtKB
  • protein domain specific binding Source: UniProtKB
  • protein phosphatase 2A binding Source: GO_Central

Protein-protein interaction databases

BioGridi202041. 5 interactions.
IntActiQ61249. 11 interactions.
MINTiMINT-4597261.
STRINGi10090.ENSMUSP00000033570.

Structurei

Secondary structure

1
340
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi13 – 2917Combined sources
Helixi37 – 6024Combined sources
Turni61 – 633Combined sources
Beta strandi65 – 673Combined sources
Turni74 – 763Combined sources
Helixi77 – 815Combined sources
Helixi82 – 909Combined sources
Helixi96 – 983Combined sources
Helixi99 – 11416Combined sources
Helixi147 – 18135Combined sources
Helixi187 – 21933Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3QC1X-ray2.35A1-223[»]
ProteinModelPortaliQ61249.
SMRiQ61249. Positions 10-222.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ61249.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini47 – 6115UIMCuratedAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni99 – 203105Interaction with PPP2CABy similarityAdd
BLAST
Regioni226 – 29166Interaction with MID1By similarityAdd
BLAST

Domaini

The UIM domain is required for protective effect on PP2A.

Sequence similaritiesi

Belongs to the IGBP1/TAP42 family.Curated

Phylogenomic databases

eggNOGiKOG2830. Eukaryota.
ENOG41110HD. LUCA.
GeneTreeiENSGT00390000002414.
HOGENOMiHOG000038526.
HOVERGENiHBG052090.
InParanoidiQ61249.
KOiK17606.
OMAiNPKGWGN.
OrthoDBiEOG7992R2.
PhylomeDBiQ61249.
TreeFamiTF313433.

Family and domain databases

InterProiIPR007304. TAP42-like.
[Graphical view]
PANTHERiPTHR10933. PTHR10933. 1 hit.
PfamiPF04177. TAP42. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q61249-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAASEDELLL PRLPELFETS KKLLEDVEVA TEPTGSRTIQ DKVSKGLELL
60 70 80 90 100
EKAAGMLSQL DLFSRNEDLE EIASTDLKYL MVPALQGALT MKQVNPSKRL
110 120 130 140 150
DHLQRAREHF VHFLTQCHCY HVAEFQLPQT KTNSAENNTA SSSMAYPNLV
160 170 180 190 200
AMASQRQAKI ERYKQKKEVE HRLSALKSAV ESGQADDERV REYHLLHLRR
210 220 230 240 250
WIAVSLEELE SIDQEIKILK EKDSPREETA CHSSLPEKPP MKPFILTRNK
260 270 280 290 300
AQAKVFGTGY PSLATMTVSD WYEQHQKYGV LPDRGIAKPA SADFQRAAQQ
310 320 330 340
QEDQEQKDEE SEEKALHRMR EWDDWKDTHP RGYGNRQNMG
Length:340
Mass (Da):38,971
Last modified:November 1, 1996 - v1
Checksum:i9C6BEF7C2E56BAE4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L31652 mRNA. Translation: AAA67891.1.
AJ000633 Genomic DNA. Translation: CAA04190.1.
AJ223156
, AJ223157, AJ223158, AJ223159, AJ223160 Genomic DNA. Translation: CAA11135.1.
AJ000634 Genomic DNA. Translation: CAA04191.1.
CCDSiCCDS30302.1.
PIRiI49451.
RefSeqiNP_032810.4. NM_008784.3.
UniGeneiMm.7454.

Genome annotation databases

EnsembliENSMUST00000033570; ENSMUSP00000033570; ENSMUSG00000031221.
GeneIDi18518.
KEGGimmu:18518.
UCSCiuc009tvz.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L31652 mRNA. Translation: AAA67891.1.
AJ000633 Genomic DNA. Translation: CAA04190.1.
AJ223156
, AJ223157, AJ223158, AJ223159, AJ223160 Genomic DNA. Translation: CAA11135.1.
AJ000634 Genomic DNA. Translation: CAA04191.1.
CCDSiCCDS30302.1.
PIRiI49451.
RefSeqiNP_032810.4. NM_008784.3.
UniGeneiMm.7454.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3QC1X-ray2.35A1-223[»]
ProteinModelPortaliQ61249.
SMRiQ61249. Positions 10-222.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi202041. 5 interactions.
IntActiQ61249. 11 interactions.
MINTiMINT-4597261.
STRINGi10090.ENSMUSP00000033570.

PTM databases

iPTMnetiQ61249.
PhosphoSiteiQ61249.

2D gel databases

REPRODUCTION-2DPAGEQ61249.

Proteomic databases

EPDiQ61249.
MaxQBiQ61249.
PaxDbiQ61249.
PeptideAtlasiQ61249.
PRIDEiQ61249.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000033570; ENSMUSP00000033570; ENSMUSG00000031221.
GeneIDi18518.
KEGGimmu:18518.
UCSCiuc009tvz.2. mouse.

Organism-specific databases

CTDi3476.
MGIiMGI:1346500. Igbp1.

Phylogenomic databases

eggNOGiKOG2830. Eukaryota.
ENOG41110HD. LUCA.
GeneTreeiENSGT00390000002414.
HOGENOMiHOG000038526.
HOVERGENiHBG052090.
InParanoidiQ61249.
KOiK17606.
OMAiNPKGWGN.
OrthoDBiEOG7992R2.
PhylomeDBiQ61249.
TreeFamiTF313433.

Miscellaneous databases

ChiTaRSiIgbp1. mouse.
EvolutionaryTraceiQ61249.
PROiQ61249.
SOURCEiSearch...

Gene expression databases

BgeeiQ61249.
CleanExiMM_IGBP1.
GenevisibleiQ61249. MM.

Family and domain databases

InterProiIPR007304. TAP42-like.
[Graphical view]
PANTHERiPTHR10933. PTHR10933. 1 hit.
PfamiPF04177. TAP42. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning of a cDNA clone encoding a phosphoprotein component related to the Ig receptor-mediated signal transduction."
    Inui S., Kuwahara K., Mizutani J., Maeda K., Kawai T., Nakayasu H., Sakaguchi N.
    J. Immunol. 154:2714-2723(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: NZC.
    Tissue: B-cell.
  2. "The gene structure and promoter analysis of mouse lymphocyte signal transduction molecule alpha 4 that is related to the yeast TAP42 involved in a rapamycin-sensitive pathway."
    Maeda K., Inui S., Sanjo H., Sakaguchi N.
    Gene 210:287-295(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-63.
    Strain: BALB/cJ.
    Tissue: Liver.
  3. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Brown adipose tissue, Heart, Kidney, Liver, Lung, Pancreas and Spleen.
  4. "The E3 ubiquitin ligase- and protein phosphatase 2A (PP2A)-binding domains of the Alpha4 protein are both required for Alpha4 to inhibit PP2A degradation."
    LeNoue-Newton M., Watkins G.R., Zou P., Germane K.L., McCorvey L.R., Wadzinski B.E., Spiller B.W.
    J. Biol. Chem. 286:17665-17671(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.35 ANGSTROMS) OF 1-223, INTERACTION WITH MID1 AND PPP2CA.

Entry informationi

Entry nameiIGBP1_MOUSE
AccessioniPrimary (citable) accession number: Q61249
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 2001
Last sequence update: November 1, 1996
Last modified: July 6, 2016
This is version 121 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.