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Q61247 (A2AP_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 115. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Alpha-2-antiplasmin

Short name=Alpha-2-AP
Alternative name(s):
Alpha-2-plasmin inhibitor
Short name=Alpha-2-PI
Serpin F2
Gene names
Name:Serpinf2
Synonyms:Pli
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length491 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Serine protease inhibitor. The major targets of this inhibitor are plasmin and trypsin, but it also inactivates matriptase-3/TMPRSS7 and chymotrypsin By similarity.

Subunit structure

Forms protease inhibiting heterodimer with TMPRSS7 By similarity.

Subcellular location

Secreted.

Tissue specificity

Expressed by the liver and secreted in plasma.

Sequence similarities

Belongs to the serpin family.

Ontologies

Keywords
   Biological processAcute phase
   Cellular componentSecreted
   DomainSignal
   Molecular functionProtease inhibitor
Serine protease inhibitor
   PTMDisulfide bond
Glycoprotein
Sulfation
   Technical term3D-structure
Complete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Biological_processacute-phase response

Inferred from electronic annotation. Source: UniProtKB-KW

blood vessel morphogenesis

Inferred from mutant phenotype PubMed 18436805. Source: BHF-UCL

collagen fibril organization

Inferred from mutant phenotype PubMed 17958745. Source: BHF-UCL

negative regulation of endopeptidase activity

Inferred from Biological aspect of Ancestor. Source: RefGenome

negative regulation of fibrinolysis

Inferred from electronic annotation. Source: Ensembl

negative regulation of plasminogen activation

Inferred from electronic annotation. Source: Ensembl

positive regulation of ERK1 and ERK2 cascade

Inferred from electronic annotation. Source: Ensembl

positive regulation of JNK cascade

Inferred from electronic annotation. Source: Ensembl

positive regulation of cell differentiation

Inferred from mutant phenotype PubMed 20008146. Source: BHF-UCL

positive regulation of collagen biosynthetic process

Inferred from mutant phenotype PubMed 18436805. Source: BHF-UCL

positive regulation of smooth muscle cell proliferation

Inferred from mutant phenotype PubMed 18436805. Source: BHF-UCL

positive regulation of stress fiber assembly

Inferred from electronic annotation. Source: Ensembl

positive regulation of transcription from RNA polymerase II promoter

Inferred from mutant phenotype PubMed 17958745. Source: BHF-UCL

positive regulation of transforming growth factor beta production

Inferred from mutant phenotype PubMed 17958745. Source: BHF-UCL

regulation of blood vessel size by renin-angiotensin

Inferred from mutant phenotype PubMed 18436805. Source: BHF-UCL

regulation of proteolysis

Inferred from Biological aspect of Ancestor. Source: RefGenome

response to organic substance

Inferred from electronic annotation. Source: Ensembl

   Cellular_componentcell surface

Inferred from electronic annotation. Source: Ensembl

extracellular space

Inferred from Biological aspect of Ancestor. Source: RefGenome

fibrinogen complex

Inferred from electronic annotation. Source: Ensembl

   Molecular_functionserine-type endopeptidase inhibitor activity

Inferred from Biological aspect of Ancestor. Source: RefGenome

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2727 Ref.3
Chain28 – 491464Alpha-2-antiplasmin
PRO_0000032513

Sites

Site403 – 4042Reactive bond for plasmin
Site404 – 4052Reactive bond for chymotrypsin By similarity

Amino acid modifications

Modified residue4841Sulfotyrosine By similarity
Glycosylation1261N-linked (GlcNAc...) Potential
Glycosylation2951N-linked (GlcNAc...) Potential
Glycosylation3091N-linked (GlcNAc...) Potential
Glycosylation3161N-linked (GlcNAc...) Potential
Disulfide bond70 ↔ 143 By similarity

Secondary structure

..................................................... 491
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q61247 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: B828DECADF0BB5B4

FASTA49154,972
        10         20         30         40         50         60 
MALLRGLLVL SLSCLQGPCF TFSPVSAVDL PGQQPVSEQA QQKLPLPALF KLDNQDFGDH 

        70         80         90        100        110        120 
ATLKRSPGHC KSVPTAEETR RLAQAMMAFT TDLFSLVAQT STSSNLVLSP LSVALALSHL 

       130        140        150        160        170        180 
ALGAQNQTLH SLHRVLHMNT GSCLPHLLSH FYQNLGPGTI RLAARIYLQK GFPIKDDFLE 

       190        200        210        220        230        240 
QSERLFGAKP VKLTGKQEED LANINQWVKE ATEGKIEDFL SELPDSTVLL LLNAIHFHGF 

       250        260        270        280        290        300 
WRTKFDPSLT QKDFFHLDER FTVSVDMMHA VSYPLRWFLL EQPEIQVAHF PFKNNMSFVV 

       310        320        330        340        350        360 
VMPTYFEWNV SEVLANLTWD TLYHPSLQER PTKVWLPKLH LQQQLDLVAT LSQLGLQELF 

       370        380        390        400        410        420 
QGPDLRGISE QNLVVSSVQH QSTMELSEAG VEAAAATSVA MNRMSLSSFT VNRPFLFFIM 

       430        440        450        460        470        480 
EDTIGVPLFV GSVRNPNPSA LPQLQEQRDS PDNRLIGQND KADFHGGKTF GPDLKLAPRM 

       490 
EEDYPQFSSP K 

« Hide

References

« Hide 'large scale' references
[1]"The kidney is a major site of alpha(2)-antiplasmin production."
Menoud P.-A., Sappino N., Boudal-Khoshbeen M., Vassalli J.-D., Sappino A.P.
J. Clin. Invest. 97:2478-2484(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: C57BL/6 X CBA.
Tissue: Liver.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: FVB/N.
Tissue: Liver.
[3]"Characterization of the murine plasma fibrinolytic system."
Lijnen H.R., van Hoef B., Beelen V., Collen D.
Eur. J. Biochem. 224:863-871(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 28-33.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z36774 mRNA. Translation: CAA85350.1.
BC026756 mRNA. Translation: AAH26756.1.
PIRS47217.
RefSeqNP_032904.1. NM_008878.2.
UniGeneMm.279733.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2R9YX-ray2.65A71-491[»]
ProteinModelPortalQ61247.
SMRQ61247. Positions 33-446.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActQ61247. 1 interaction.
MINTMINT-4086385.

Protein family/group databases

MEROPSI04.023.

PTM databases

PhosphoSiteQ61247.

Proteomic databases

PaxDbQ61247.
PRIDEQ61247.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000043696; ENSMUSP00000048704; ENSMUSG00000038224.
ENSMUST00000108437; ENSMUSP00000104076; ENSMUSG00000038224.
GeneID18816.
KEGGmmu:18816.
UCSCuc007kdr.2. mouse.

Organism-specific databases

CTD5345.
MGIMGI:107173. Serpinf2.

Phylogenomic databases

eggNOGCOG4826.
GeneTreeENSGT00750000117478.
HOGENOMHOG000231761.
HOVERGENHBG000043.
InParanoidQ61247.
KOK03983.
OMARWFLLEQ.
OrthoDBEOG7WT41R.
PhylomeDBQ61247.
TreeFamTF317350.

Gene expression databases

ArrayExpressQ61247.
BgeeQ61247.
GenevestigatorQ61247.

Family and domain databases

InterProIPR023795. Serpin_CS.
IPR023796. Serpin_dom.
IPR000215. Serpin_fam.
[Graphical view]
PANTHERPTHR11461. PTHR11461. 1 hit.
PfamPF00079. Serpin. 1 hit.
[Graphical view]
SMARTSM00093. SERPIN. 1 hit.
[Graphical view]
SUPFAMSSF56574. SSF56574. 2 hits.
PROSITEPS00284. SERPIN. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceQ61247.
NextBio295170.
PROQ61247.
SOURCESearch...

Entry information

Entry nameA2AP_MOUSE
AccessionPrimary (citable) accession number: Q61247
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: April 16, 2014
This is version 115 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot