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Protein

Alpha-2-antiplasmin

Gene

Serpinf2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Serine protease inhibitor. The major targets of this inhibitor are plasmin and trypsin, but it also inactivates matriptase-3/TMPRSS7 and chymotrypsin (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei403 – 404Reactive bond for plasmin2
Sitei404 – 405Reactive bond for chymotrypsinBy similarity2

GO - Molecular functioni

GO - Biological processi

  • acute-phase response Source: UniProtKB-KW
  • blood vessel morphogenesis Source: BHF-UCL
  • collagen fibril organization Source: BHF-UCL
  • negative regulation of fibrinolysis Source: MGI
  • negative regulation of plasminogen activation Source: MGI
  • positive regulation of cell differentiation Source: BHF-UCL
  • positive regulation of collagen biosynthetic process Source: BHF-UCL
  • positive regulation of ERK1 and ERK2 cascade Source: MGI
  • positive regulation of JNK cascade Source: MGI
  • positive regulation of smooth muscle cell proliferation Source: BHF-UCL
  • positive regulation of stress fiber assembly Source: MGI
  • positive regulation of transcription from RNA polymerase II promoter Source: BHF-UCL
  • positive regulation of transforming growth factor beta production Source: BHF-UCL
  • regulation of blood vessel size by renin-angiotensin Source: BHF-UCL
  • response to organic substance Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Protease inhibitor, Serine protease inhibitor

Keywords - Biological processi

Acute phase

Enzyme and pathway databases

ReactomeiR-MMU-114608. Platelet degranulation.
R-MMU-75205. Dissolution of Fibrin Clot.

Protein family/group databases

MEROPSiI04.023.

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-2-antiplasmin
Short name:
Alpha-2-AP
Alternative name(s):
Alpha-2-plasmin inhibitor
Short name:
Alpha-2-PI
Serpin F2
Gene namesi
Name:Serpinf2
Synonyms:Pli
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:107173. Serpinf2.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 271 PublicationAdd BLAST27
PropeptideiPRO_000043066828 – 391 PublicationAdd BLAST12
ChainiPRO_000003251340 – 491Alpha-2-antiplasminAdd BLAST452

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi70 ↔ 143By similarity
Glycosylationi126N-linked (GlcNAc...)Sequence analysis1
Glycosylationi295N-linked (GlcNAc...)Sequence analysis1
Glycosylationi309N-linked (GlcNAc...)Sequence analysis1
Glycosylationi316N-linked (GlcNAc...)Sequence analysis1
Modified residuei484SulfotyrosineBy similarity1

Post-translational modificationi

Proteolytically cleaved at Pro-35 by both the prolyl endopeptidase FAP form and antiplasmin-cleaving enzyme FAP soluble form to generate mature alpha-2-antiplasmin.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei39 – 40Cleavage; by prolyl endopeptidase FAP, antiplasmin-cleaving enzyme FAP soluble formBy similarity2

Keywords - PTMi

Disulfide bond, Glycoprotein, Sulfation

Proteomic databases

MaxQBiQ61247.
PaxDbiQ61247.
PeptideAtlasiQ61247.
PRIDEiQ61247.

PTM databases

iPTMnetiQ61247.
PhosphoSitePlusiQ61247.

Expressioni

Tissue specificityi

Expressed by the liver and secreted in plasma.

Gene expression databases

BgeeiENSMUSG00000038224.
ExpressionAtlasiQ61247. baseline and differential.
GenevisibleiQ61247. MM.

Interactioni

Subunit structurei

Forms protease inhibiting heterodimer with TMPRSS7.By similarity

GO - Molecular functioni

Protein-protein interaction databases

IntActiQ61247. 1 interactor.
MINTiMINT-4086385.
STRINGi10090.ENSMUSP00000048704.

Structurei

Secondary structure

1491
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi76 – 100Combined sources25
Beta strandi103 – 108Combined sources6
Helixi110 – 123Combined sources14
Helixi126 – 135Combined sources10
Helixi144 – 153Combined sources10
Beta strandi157 – 168Combined sources12
Helixi176 – 186Combined sources11
Helixi197 – 211Combined sources15
Turni212 – 214Combined sources3
Beta strandi228 – 238Combined sources11
Beta strandi241 – 243Combined sources3
Helixi247 – 249Combined sources3
Beta strandi251 – 280Combined sources30
Turni281 – 284Combined sources4
Beta strandi285 – 306Combined sources22
Helixi310 – 314Combined sources5
Helixi319 – 322Combined sources4
Beta strandi330 – 337Combined sources8
Beta strandi339 – 346Combined sources8
Helixi348 – 354Combined sources7
Helixi357 – 360Combined sources4
Turni366 – 368Combined sources3
Beta strandi375 – 386Combined sources12
Beta strandi390 – 392Combined sources3
Beta strandi407 – 410Combined sources4
Beta strandi415 – 421Combined sources7
Turni422 – 424Combined sources3
Beta strandi427 – 434Combined sources8

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2R9YX-ray2.65A71-491[»]
ProteinModelPortaliQ61247.
SMRiQ61247.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ61247.

Family & Domainsi

Sequence similaritiesi

Belongs to the serpin family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG2392. Eukaryota.
COG4826. LUCA.
GeneTreeiENSGT00850000132312.
HOGENOMiHOG000231761.
HOVERGENiHBG000043.
InParanoidiQ61247.
KOiK03983.
OMAiWRSKFDP.
OrthoDBiEOG091G066U.
PhylomeDBiQ61247.
TreeFamiTF317350.

Family and domain databases

CDDicd02053. alpha2AP. 1 hit.
InterProiIPR033833. Alpha2AP.
IPR023795. Serpin_CS.
IPR023796. Serpin_dom.
IPR000215. Serpin_fam.
[Graphical view]
PANTHERiPTHR11461. PTHR11461. 1 hit.
PfamiPF00079. Serpin. 1 hit.
[Graphical view]
SMARTiSM00093. SERPIN. 1 hit.
[Graphical view]
SUPFAMiSSF56574. SSF56574. 2 hits.
PROSITEiPS00284. SERPIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q61247-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALLRGLLVL SLSCLQGPCF TFSPVSAVDL PGQQPVSEQA QQKLPLPALF
60 70 80 90 100
KLDNQDFGDH ATLKRSPGHC KSVPTAEETR RLAQAMMAFT TDLFSLVAQT
110 120 130 140 150
STSSNLVLSP LSVALALSHL ALGAQNQTLH SLHRVLHMNT GSCLPHLLSH
160 170 180 190 200
FYQNLGPGTI RLAARIYLQK GFPIKDDFLE QSERLFGAKP VKLTGKQEED
210 220 230 240 250
LANINQWVKE ATEGKIEDFL SELPDSTVLL LLNAIHFHGF WRTKFDPSLT
260 270 280 290 300
QKDFFHLDER FTVSVDMMHA VSYPLRWFLL EQPEIQVAHF PFKNNMSFVV
310 320 330 340 350
VMPTYFEWNV SEVLANLTWD TLYHPSLQER PTKVWLPKLH LQQQLDLVAT
360 370 380 390 400
LSQLGLQELF QGPDLRGISE QNLVVSSVQH QSTMELSEAG VEAAAATSVA
410 420 430 440 450
MNRMSLSSFT VNRPFLFFIM EDTIGVPLFV GSVRNPNPSA LPQLQEQRDS
460 470 480 490
PDNRLIGQND KADFHGGKTF GPDLKLAPRM EEDYPQFSSP K
Length:491
Mass (Da):54,972
Last modified:November 1, 1996 - v1
Checksum:iB828DECADF0BB5B4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z36774 mRNA. Translation: CAA85350.1.
BC026756 mRNA. Translation: AAH26756.1.
CCDSiCCDS25046.1.
PIRiS47217.
RefSeqiNP_032904.1. NM_008878.2.
UniGeneiMm.279733.

Genome annotation databases

EnsembliENSMUST00000043696; ENSMUSP00000048704; ENSMUSG00000038224.
ENSMUST00000108437; ENSMUSP00000104076; ENSMUSG00000038224.
GeneIDi18816.
KEGGimmu:18816.
UCSCiuc007kdr.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z36774 mRNA. Translation: CAA85350.1.
BC026756 mRNA. Translation: AAH26756.1.
CCDSiCCDS25046.1.
PIRiS47217.
RefSeqiNP_032904.1. NM_008878.2.
UniGeneiMm.279733.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2R9YX-ray2.65A71-491[»]
ProteinModelPortaliQ61247.
SMRiQ61247.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ61247. 1 interactor.
MINTiMINT-4086385.
STRINGi10090.ENSMUSP00000048704.

Protein family/group databases

MEROPSiI04.023.

PTM databases

iPTMnetiQ61247.
PhosphoSitePlusiQ61247.

Proteomic databases

MaxQBiQ61247.
PaxDbiQ61247.
PeptideAtlasiQ61247.
PRIDEiQ61247.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000043696; ENSMUSP00000048704; ENSMUSG00000038224.
ENSMUST00000108437; ENSMUSP00000104076; ENSMUSG00000038224.
GeneIDi18816.
KEGGimmu:18816.
UCSCiuc007kdr.2. mouse.

Organism-specific databases

CTDi5345.
MGIiMGI:107173. Serpinf2.

Phylogenomic databases

eggNOGiKOG2392. Eukaryota.
COG4826. LUCA.
GeneTreeiENSGT00850000132312.
HOGENOMiHOG000231761.
HOVERGENiHBG000043.
InParanoidiQ61247.
KOiK03983.
OMAiWRSKFDP.
OrthoDBiEOG091G066U.
PhylomeDBiQ61247.
TreeFamiTF317350.

Enzyme and pathway databases

ReactomeiR-MMU-114608. Platelet degranulation.
R-MMU-75205. Dissolution of Fibrin Clot.

Miscellaneous databases

ChiTaRSiSerpinf2. mouse.
EvolutionaryTraceiQ61247.
PROiQ61247.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000038224.
ExpressionAtlasiQ61247. baseline and differential.
GenevisibleiQ61247. MM.

Family and domain databases

CDDicd02053. alpha2AP. 1 hit.
InterProiIPR033833. Alpha2AP.
IPR023795. Serpin_CS.
IPR023796. Serpin_dom.
IPR000215. Serpin_fam.
[Graphical view]
PANTHERiPTHR11461. PTHR11461. 1 hit.
PfamiPF00079. Serpin. 1 hit.
[Graphical view]
SMARTiSM00093. SERPIN. 1 hit.
[Graphical view]
SUPFAMiSSF56574. SSF56574. 2 hits.
PROSITEiPS00284. SERPIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiA2AP_MOUSE
AccessioniPrimary (citable) accession number: Q61247
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: November 30, 2016
This is version 141 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.