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Protein

Alpha-2-antiplasmin

Gene

Serpinf2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Serine protease inhibitor. The major targets of this inhibitor are plasmin and trypsin, but it also inactivates matriptase-3/TMPRSS7 and chymotrypsin (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei39 – 402Cleavage; by prolyl endopeptidase FAP, antiplasmin-cleaving enzyme FAP soluble formBy similarity
Sitei403 – 4042Reactive bond for plasmin
Sitei404 – 4052Reactive bond for chymotrypsinBy similarity

GO - Molecular functioni

  1. protease binding Source: MGI
  2. protein homodimerization activity Source: MGI
  3. serine-type endopeptidase inhibitor activity Source: MGI

GO - Biological processi

  1. acute-phase response Source: UniProtKB-KW
  2. blood vessel morphogenesis Source: BHF-UCL
  3. collagen fibril organization Source: BHF-UCL
  4. negative regulation of endopeptidase activity Source: GO_Central
  5. negative regulation of fibrinolysis Source: MGI
  6. negative regulation of plasminogen activation Source: MGI
  7. positive regulation of cell differentiation Source: BHF-UCL
  8. positive regulation of collagen biosynthetic process Source: BHF-UCL
  9. positive regulation of ERK1 and ERK2 cascade Source: MGI
  10. positive regulation of JNK cascade Source: MGI
  11. positive regulation of smooth muscle cell proliferation Source: BHF-UCL
  12. positive regulation of stress fiber assembly Source: MGI
  13. positive regulation of transcription from RNA polymerase II promoter Source: BHF-UCL
  14. positive regulation of transforming growth factor beta production Source: BHF-UCL
  15. regulation of blood vessel size by renin-angiotensin Source: BHF-UCL
  16. regulation of proteolysis Source: GO_Central
  17. response to organic substance Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Protease inhibitor, Serine protease inhibitor

Keywords - Biological processi

Acute phase

Enzyme and pathway databases

ReactomeiREACT_235291. Dissolution of Fibrin Clot.

Protein family/group databases

MEROPSiI04.023.

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-2-antiplasmin
Short name:
Alpha-2-AP
Alternative name(s):
Alpha-2-plasmin inhibitor
Short name:
Alpha-2-PI
Serpin F2
Gene namesi
Name:Serpinf2
Synonyms:Pli
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 11

Organism-specific databases

MGIiMGI:107173. Serpinf2.

Subcellular locationi

GO - Cellular componenti

  1. blood microparticle Source: MGI
  2. cell surface Source: MGI
  3. extracellular space Source: MGI
  4. extracellular vesicular exosome Source: MGI
  5. fibrinogen complex Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 27271 PublicationAdd
BLAST
Propeptidei28 – 39121 PublicationPRO_0000430668Add
BLAST
Chaini40 – 491452Alpha-2-antiplasminPRO_0000032513Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi70 ↔ 143By similarity
Glycosylationi126 – 1261N-linked (GlcNAc...)Sequence Analysis
Glycosylationi295 – 2951N-linked (GlcNAc...)Sequence Analysis
Glycosylationi309 – 3091N-linked (GlcNAc...)Sequence Analysis
Glycosylationi316 – 3161N-linked (GlcNAc...)Sequence Analysis
Modified residuei484 – 4841SulfotyrosineBy similarity

Post-translational modificationi

Proteolytically cleaved at Pro-35 by both the prolyl endopeptidase FAP form and antiplasmin-cleaving enzyme FAP soluble form to generate mature alpha-2-antiplasmin.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Sulfation

Proteomic databases

MaxQBiQ61247.
PaxDbiQ61247.
PRIDEiQ61247.

PTM databases

PhosphoSiteiQ61247.

Expressioni

Tissue specificityi

Expressed by the liver and secreted in plasma.

Gene expression databases

BgeeiQ61247.
ExpressionAtlasiQ61247. baseline and differential.
GenevestigatoriQ61247.

Interactioni

Subunit structurei

Forms protease inhibiting heterodimer with TMPRSS7.By similarity

Protein-protein interaction databases

IntActiQ61247. 1 interaction.
MINTiMINT-4086385.

Structurei

Secondary structure

1
491
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi76 – 10025Combined sources
Beta strandi103 – 1086Combined sources
Helixi110 – 12314Combined sources
Helixi126 – 13510Combined sources
Helixi144 – 15310Combined sources
Beta strandi157 – 16812Combined sources
Helixi176 – 18611Combined sources
Helixi197 – 21115Combined sources
Turni212 – 2143Combined sources
Beta strandi228 – 23811Combined sources
Beta strandi241 – 2433Combined sources
Helixi247 – 2493Combined sources
Beta strandi251 – 28030Combined sources
Turni281 – 2844Combined sources
Beta strandi285 – 30622Combined sources
Helixi310 – 3145Combined sources
Helixi319 – 3224Combined sources
Beta strandi330 – 3378Combined sources
Beta strandi339 – 3468Combined sources
Helixi348 – 3547Combined sources
Helixi357 – 3604Combined sources
Turni366 – 3683Combined sources
Beta strandi375 – 38612Combined sources
Beta strandi390 – 3923Combined sources
Beta strandi407 – 4104Combined sources
Beta strandi415 – 4217Combined sources
Turni422 – 4243Combined sources
Beta strandi427 – 4348Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2R9YX-ray2.65A71-491[»]
ProteinModelPortaliQ61247.
SMRiQ61247. Positions 73-446.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ61247.

Family & Domainsi

Sequence similaritiesi

Belongs to the serpin family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG4826.
GeneTreeiENSGT00770000120524.
HOGENOMiHOG000231761.
HOVERGENiHBG000043.
InParanoidiQ61247.
KOiK03983.
OMAiWFLLEQP.
OrthoDBiEOG7WT41R.
PhylomeDBiQ61247.
TreeFamiTF317350.

Family and domain databases

InterProiIPR023795. Serpin_CS.
IPR023796. Serpin_dom.
IPR000215. Serpin_fam.
[Graphical view]
PANTHERiPTHR11461. PTHR11461. 1 hit.
PfamiPF00079. Serpin. 1 hit.
[Graphical view]
SMARTiSM00093. SERPIN. 1 hit.
[Graphical view]
SUPFAMiSSF56574. SSF56574. 2 hits.
PROSITEiPS00284. SERPIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q61247-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MALLRGLLVL SLSCLQGPCF TFSPVSAVDL PGQQPVSEQA QQKLPLPALF
60 70 80 90 100
KLDNQDFGDH ATLKRSPGHC KSVPTAEETR RLAQAMMAFT TDLFSLVAQT
110 120 130 140 150
STSSNLVLSP LSVALALSHL ALGAQNQTLH SLHRVLHMNT GSCLPHLLSH
160 170 180 190 200
FYQNLGPGTI RLAARIYLQK GFPIKDDFLE QSERLFGAKP VKLTGKQEED
210 220 230 240 250
LANINQWVKE ATEGKIEDFL SELPDSTVLL LLNAIHFHGF WRTKFDPSLT
260 270 280 290 300
QKDFFHLDER FTVSVDMMHA VSYPLRWFLL EQPEIQVAHF PFKNNMSFVV
310 320 330 340 350
VMPTYFEWNV SEVLANLTWD TLYHPSLQER PTKVWLPKLH LQQQLDLVAT
360 370 380 390 400
LSQLGLQELF QGPDLRGISE QNLVVSSVQH QSTMELSEAG VEAAAATSVA
410 420 430 440 450
MNRMSLSSFT VNRPFLFFIM EDTIGVPLFV GSVRNPNPSA LPQLQEQRDS
460 470 480 490
PDNRLIGQND KADFHGGKTF GPDLKLAPRM EEDYPQFSSP K
Length:491
Mass (Da):54,972
Last modified:November 1, 1996 - v1
Checksum:iB828DECADF0BB5B4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z36774 mRNA. Translation: CAA85350.1.
BC026756 mRNA. Translation: AAH26756.1.
CCDSiCCDS25046.1.
PIRiS47217.
RefSeqiNP_032904.1. NM_008878.2.
UniGeneiMm.279733.

Genome annotation databases

EnsembliENSMUST00000043696; ENSMUSP00000048704; ENSMUSG00000038224.
ENSMUST00000108437; ENSMUSP00000104076; ENSMUSG00000038224.
GeneIDi18816.
KEGGimmu:18816.
UCSCiuc007kdr.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z36774 mRNA. Translation: CAA85350.1.
BC026756 mRNA. Translation: AAH26756.1.
CCDSiCCDS25046.1.
PIRiS47217.
RefSeqiNP_032904.1. NM_008878.2.
UniGeneiMm.279733.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2R9YX-ray2.65A71-491[»]
ProteinModelPortaliQ61247.
SMRiQ61247. Positions 73-446.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ61247. 1 interaction.
MINTiMINT-4086385.

Protein family/group databases

MEROPSiI04.023.

PTM databases

PhosphoSiteiQ61247.

Proteomic databases

MaxQBiQ61247.
PaxDbiQ61247.
PRIDEiQ61247.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000043696; ENSMUSP00000048704; ENSMUSG00000038224.
ENSMUST00000108437; ENSMUSP00000104076; ENSMUSG00000038224.
GeneIDi18816.
KEGGimmu:18816.
UCSCiuc007kdr.2. mouse.

Organism-specific databases

CTDi5345.
MGIiMGI:107173. Serpinf2.

Phylogenomic databases

eggNOGiCOG4826.
GeneTreeiENSGT00770000120524.
HOGENOMiHOG000231761.
HOVERGENiHBG000043.
InParanoidiQ61247.
KOiK03983.
OMAiWFLLEQP.
OrthoDBiEOG7WT41R.
PhylomeDBiQ61247.
TreeFamiTF317350.

Enzyme and pathway databases

ReactomeiREACT_235291. Dissolution of Fibrin Clot.

Miscellaneous databases

ChiTaRSiSerpinf2. mouse.
EvolutionaryTraceiQ61247.
NextBioi295170.
PROiQ61247.
SOURCEiSearch...

Gene expression databases

BgeeiQ61247.
ExpressionAtlasiQ61247. baseline and differential.
GenevestigatoriQ61247.

Family and domain databases

InterProiIPR023795. Serpin_CS.
IPR023796. Serpin_dom.
IPR000215. Serpin_fam.
[Graphical view]
PANTHERiPTHR11461. PTHR11461. 1 hit.
PfamiPF00079. Serpin. 1 hit.
[Graphical view]
SMARTiSM00093. SERPIN. 1 hit.
[Graphical view]
SUPFAMiSSF56574. SSF56574. 2 hits.
PROSITEiPS00284. SERPIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: C57BL/6 X CBA.
    Tissue: Liver.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Liver.
  3. "Characterization of the murine plasma fibrinolytic system."
    Lijnen H.R., van Hoef B., Beelen V., Collen D.
    Eur. J. Biochem. 224:863-871(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 28-33.

Entry informationi

Entry nameiA2AP_MOUSE
AccessioniPrimary (citable) accession number: Q61247
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: February 4, 2015
This is version 123 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.