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Protein

Testis-specific serine/threonine-protein kinase 1

Gene

Tssk1b

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Testis-specific serine/threonine-protein kinase required during spermatid development. Phosphorylates 'Ser-281' of TSKS. Involved in the late stages of spermatogenesis, during the reconstruction of the cytoplasm. During spermatogenesis, required for the transformation of a ring-shaped structure around the base of the flagellum originating from the chromatoid body.1 Publication

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Cofactori

Mg2+By similarity

Enzyme regulationi

Activated by phosphorylation on Thr-174, potentially by autophosphorylation.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei41 – 411ATPPROSITE-ProRule annotation
Active sitei136 – 1361Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi18 – 269ATPPROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

  • multicellular organism development Source: UniProtKB-KW
  • protein phosphorylation Source: UniProtKB
  • spermatid development Source: UniProtKB
  • spermatogenesis Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Differentiation, Spermatogenesis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Testis-specific serine/threonine-protein kinase 1 (EC:2.7.11.1)
Short name:
TSK-1
Short name:
TSK1
Short name:
TSSK-1
Short name:
Testis-specific kinase 1
Alternative name(s):
Serine/threonine-protein kinase 22A
Gene namesi
Name:Tssk1b
Synonyms:Stk22a, Tssk1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 16

Organism-specific databases

MGIiMGI:1347557. Tssk1.

Subcellular locationi

GO - Cellular componenti

  • acrosomal vesicle Source: UniProtKB
  • motile cilium Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell projection, Cytoplasm, Cytoplasmic vesicle

Pathology & Biotechi

Disruption phenotypei

Male mice lacking Tssk1b and Tssk2 are sterile due to haploinsufficiency. chimeras show failure to form elongated spermatids, apoptosis of spermatocytes and spermatids, and the appearance of numerous round cells in the epididymal lumen. Elongating spermatids possess a collapsed mitochondrial sheath.2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 365365Testis-specific serine/threonine-protein kinase 1PRO_0000086767Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei174 – 1741PhosphothreonineBy similarity

Post-translational modificationi

Autophosphorylated.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ61241.
PRIDEiQ61241.

2D gel databases

REPRODUCTION-2DPAGEIPI00403935.

PTM databases

PhosphoSiteiQ61241.

Expressioni

Tissue specificityi

Testis-specific. Expressed only in postmeiotic spermatids at the final stages of cytodifferentiation in the seminiferous tubules (at protein level). Not detected in released sperms in the lumen of the seminiferous tubules and the epididymis.3 Publications

Gene expression databases

BgeeiQ61241.
CleanExiMM_TSSK1.
GenevisibleiQ61241. MM.

Interactioni

Subunit structurei

Interacts with TSSK2.1 Publication

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000040302.

Structurei

3D structure databases

ProteinModelPortaliQ61241.
SMRiQ61241. Positions 6-316.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini12 – 272261Protein kinasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0583. Eukaryota.
COG0515. LUCA.
GeneTreeiENSGT00770000120521.
HOGENOMiHOG000233016.
HOVERGENiHBG101110.
InParanoidiQ61241.
KOiK08811.
OMAiCWVQPKV.
OrthoDBiEOG7P8P8F.
PhylomeDBiQ61241.
TreeFamiTF352374.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q61241-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDDAAVLKRR GYIMGINLGE GSYAKVKSAY SERLKFNVAV KIIDRKKAPS
60 70 80 90 100
DFLEKFLPRE IEILAMLNHR SIVKTYEIFE TSDGKVYIVM ELGVQGDLLE
110 120 130 140 150
FIKTRGALQE DDARKKFHQL SSAIKYCHDL DVVHRDLKCE NLLLDKDFNI
160 170 180 190 200
KLSDFGFSKR CLRDDSGRLI LSKTFCGSAA YAAPEVLQGI PYQPKVYDIW
210 220 230 240 250
SLGVILYIMV CGSMPYDDSN IKKMLRIQKE HRVNFPRSKH LTGECKDLIY
260 270 280 290 300
RMLQPDVNRR LHIDEILNHC WVQPKARGLS SGAINKEGES SRATEPSWIP
310 320 330 340 350
EPGADKKSAT KLEPREEARS EARSESKPQE DTLQVVRQSE NVGLSSELNR
360
DTEEGHPQQP SETHT
Length:365
Mass (Da):41,589
Last modified:September 21, 2011 - v2
Checksum:i06CF52ABD9616A37
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti80 – 801E → A in AAA99535 (PubMed:8112611).Curated
Sequence conflicti139 – 1391C → S in AAA99535 (PubMed:8112611).Curated
Sequence conflicti224 – 2241Missing in AAA99535 (PubMed:8112611).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U01840 mRNA. Translation: AAA99535.1.
CH466521 Genomic DNA. Translation: EDK97490.1.
BC050772 mRNA. Translation: AAH50772.2.
CCDSiCCDS28011.1.
RefSeqiNP_033461.2. NM_009435.2.
UniGeneiMm.439725.

Genome annotation databases

EnsembliENSMUST00000046937; ENSMUSP00000040302; ENSMUSG00000041566.
GeneIDi22114.
KEGGimmu:22114.
UCSCiuc007ymk.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U01840 mRNA. Translation: AAA99535.1.
CH466521 Genomic DNA. Translation: EDK97490.1.
BC050772 mRNA. Translation: AAH50772.2.
CCDSiCCDS28011.1.
RefSeqiNP_033461.2. NM_009435.2.
UniGeneiMm.439725.

3D structure databases

ProteinModelPortaliQ61241.
SMRiQ61241. Positions 6-316.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000040302.

PTM databases

PhosphoSiteiQ61241.

2D gel databases

REPRODUCTION-2DPAGEIPI00403935.

Proteomic databases

PaxDbiQ61241.
PRIDEiQ61241.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000046937; ENSMUSP00000040302; ENSMUSG00000041566.
GeneIDi22114.
KEGGimmu:22114.
UCSCiuc007ymk.1. mouse.

Organism-specific databases

CTDi22114.
MGIiMGI:1347557. Tssk1.

Phylogenomic databases

eggNOGiKOG0583. Eukaryota.
COG0515. LUCA.
GeneTreeiENSGT00770000120521.
HOGENOMiHOG000233016.
HOVERGENiHBG101110.
InParanoidiQ61241.
KOiK08811.
OMAiCWVQPKV.
OrthoDBiEOG7P8P8F.
PhylomeDBiQ61241.
TreeFamiTF352374.

Miscellaneous databases

NextBioi301963.
PROiQ61241.
SOURCEiSearch...

Gene expression databases

BgeeiQ61241.
CleanExiMM_TSSK1.
GenevisibleiQ61241. MM.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Characterization of a novel murine testis-specific serine/threonine kinase."
    Bielke W., Blaschke R.J., Miescher G.C., Zurcher G., Andres A.-C., Ziemiecki A.
    Gene 139:235-239(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Testis.
  2. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Testis.
  4. "Immunohistochemical analysis of the expression of two serine-threonine kinases in the maturing mouse testis."
    Nayak S., Galili N., Buck C.A.
    Mech. Dev. 74:171-174(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
  5. "A novel family of serine/threonine kinases participating in spermiogenesis."
    Kueng P., Nikolova Z., Djonov V., Hemphill A., Rohrbach V., Boehlen D., Zuercher G., Andres A.-C., Ziemiecki A.
    J. Cell Biol. 139:1851-1859(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
  6. "A novel member of the testis specific serine kinase family, tssk-3, expressed in the Leydig cells of sexually mature mice."
    Zuercher G., Rohrbach V., Andres A.-C., Ziemiecki A.
    Mech. Dev. 93:175-177(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH TSSK2.
  7. "Targeted deletion of Tssk1 and 2 causes male infertility due to haploinsufficiency."
    Xu B., Hao Z., Jha K.N., Zhang Z., Urekar C., Digilio L., Pulido S., Strauss J.F. III, Flickinger C.J., Herr J.C.
    Dev. Biol. 319:211-222(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISRUPTION PHENOTYPE.
  8. "Functional transformation of the chromatoid body in mouse spermatids requires testis-specific serine/threonine kinases."
    Shang P., Baarends W.M., Hoogerbrugge J., Ooms M.P., van Cappellen W.A., de Jong A.A., Dohle G.R., van Eenennaam H., Gossen J.A., Grootegoed J.A.
    J. Cell Sci. 123:331-339(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISRUPTION PHENOTYPE, SUBCELLULAR LOCATION, FUNCTION.
  9. "Expression and localization of five members of the testis-specific serine kinase (Tssk) family in mouse and human sperm and testis."
    Li Y., Sosnik J., Brassard L., Reese M., Spiridonov N.A., Bates T.C., Johnson G.R., Anguita J., Visconti P.E., Salicioni A.M.
    Mol. Hum. Reprod. 17:42-56(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY.

Entry informationi

Entry nameiTSSK1_MOUSE
AccessioniPrimary (citable) accession number: Q61241
Secondary accession number(s): Q80YU1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 30, 2003
Last sequence update: September 21, 2011
Last modified: May 11, 2016
This is version 141 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.