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Protein

Plastin-2

Gene

Lcp1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Actin-binding protein. Plays a role in the activation of T-cells in response to costimulation through TCR/CD3 and CD2 or CD28. Modulates the cell surface expression of IL2RA/CD25 and CD69 (By similarity).By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Calcium bindingi22 – 331PROSITE-ProRule annotationAdd BLAST12
Calcium bindingi62 – 732PROSITE-ProRule annotationAdd BLAST12

GO - Molecular functioni

  • actin filament binding Source: MGI
  • calcium ion binding Source: InterPro
  • GTPase binding Source: MGI
  • identical protein binding Source: MGI

GO - Biological processi

Complete GO annotation...

Keywords - Ligandi

Actin-binding, Calcium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Plastin-2
Alternative name(s):
65 kDa macrophage protein
L-plastin
Lymphocyte cytosolic protein 1
Short name:
LCP-1
pp65
Gene namesi
Name:Lcp1
Synonyms:Pls2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 14

Organism-specific databases

MGIiMGI:104808. Lcp1.

Subcellular locationi

  • Cytoplasmcytoskeleton 1 Publication
  • Cell projection By similarity
  • Cell junction By similarity
  • Cell projectionruffle membrane 1 Publication; Peripheral membrane protein 1 Publication; Cytoplasmic side 1 Publication

  • Note: Relocalizes to the immunological synapse between peripheral blood T-lymphocytes and antibody-presenting cells in response to costimulation through TCR/CD3 and CD2 or CD28. Relocalizes to actin-rich cell projections upon serine phosphorylation (By similarity). Associated with the actin cytoskeleton at membrane ruffles.By similarity

GO - Cellular componenti

  • actin cytoskeleton Source: MGI
  • actin filament Source: MGI
  • actin filament bundle Source: MGI
  • cytoplasm Source: MGI
  • cytosol Source: UniProtKB
  • extracellular exosome Source: MGI
  • extracellular space Source: MGI
  • filopodium Source: MGI
  • focal adhesion Source: MGI
  • phagocytic cup Source: MGI
  • plasma membrane Source: MGI
  • podosome Source: MGI
  • ruffle Source: MGI
  • ruffle membrane Source: UniProtKB-SubCell
  • stress fiber Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Cell projection, Cytoplasm, Cytoskeleton, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00000737442 – 627Plastin-2Add BLAST626

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Modified residuei5PhosphoserineCombined sources1 Publication1
Modified residuei7Phosphoserine1 Publication1
Modified residuei28PhosphotyrosineBy similarity1
Modified residuei30PhosphoserineCombined sources1
Modified residuei76N6-acetyllysineBy similarity1
Modified residuei88N6-acetyllysineBy similarity1
Modified residuei124PhosphotyrosineBy similarity1
Modified residuei257PhosphoserineCombined sources1
Modified residuei290PhosphoserineBy similarity1
Modified residuei291PhosphothreonineBy similarity1
Modified residuei294N6-acetyllysineBy similarity1
Modified residuei297N6-acetyllysineBy similarity1
Modified residuei323PhosphoserineBy similarity1
Modified residuei353PhosphothreonineBy similarity1
Modified residuei361N6-acetyllysineBy similarity1
Modified residuei406PhosphoserineBy similarity1
Modified residuei472N6-acetyllysineBy similarity1
Modified residuei474PhosphoserineBy similarity1
Modified residuei542N6-acetyllysineBy similarity1
Modified residuei579N6-acetyllysineBy similarity1

Post-translational modificationi

Phosphorylated on a serine residue in response to costimulation through TCR/CD3 and CD2 or CD28. Serine phosphorylation promotes association with the actin cytoskeleton and targeting to peripheral cell projections (By similarity).By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ61233.
MaxQBiQ61233.
PaxDbiQ61233.
PeptideAtlasiQ61233.
PRIDEiQ61233.

2D gel databases

COMPLUYEAST-2DPAGEQ61233.
REPRODUCTION-2DPAGEQ61233.

PTM databases

iPTMnetiQ61233.
PhosphoSitePlusiQ61233.
SwissPalmiQ61233.

Expressioni

Gene expression databases

BgeeiENSMUSG00000021998.
CleanExiMM_LCP1.
ExpressionAtlasiQ61233. baseline and differential.
GenevisibleiQ61233. MM.

Interactioni

Subunit structurei

Monomer (By similarity). Interacts with AIF1.By similarity1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
Mapk14P478115EBI-309345,EBI-298727

GO - Molecular functioni

  • actin filament binding Source: MGI
  • GTPase binding Source: MGI
  • identical protein binding Source: MGI

Protein-protein interaction databases

BioGridi202256. 4 interactors.
IntActiQ61233. 7 interactors.
MINTiMINT-217293.
STRINGi10090.ENSMUSP00000116271.

Structurei

3D structure databases

ProteinModelPortaliQ61233.
SMRiQ61233.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini9 – 44EF-hand 1PROSITE-ProRule annotationAdd BLAST36
Domaini49 – 84EF-hand 2PROSITE-ProRule annotationAdd BLAST36
Domaini106 – 379Actin-binding 1Add BLAST274
Domaini120 – 236CH 1PROSITE-ProRule annotationAdd BLAST117
Domaini264 – 375CH 2PROSITE-ProRule annotationAdd BLAST112
Domaini380 – 624Actin-binding 2Add BLAST245
Domaini394 – 503CH 3PROSITE-ProRule annotationAdd BLAST110
Domaini515 – 624CH 4PROSITE-ProRule annotationAdd BLAST110

Sequence similaritiesi

Contains 2 actin-binding domains.Curated
Contains 4 CH (calponin-homology) domains.PROSITE-ProRule annotation
Contains 2 EF-hand domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0046. Eukaryota.
COG5069. LUCA.
GeneTreeiENSGT00390000005691.
HOGENOMiHOG000213447.
HOVERGENiHBG003082.
InParanoidiQ61233.
KOiK17276.
OMAiQPGSIKY.
OrthoDBiEOG091G03YP.
PhylomeDBiQ61233.
TreeFamiTF300680.

Family and domain databases

CDDicd00014. CH. 2 hits.
cd00051. EFh. 1 hit.
Gene3Di1.10.238.10. 1 hit.
1.10.418.10. 4 hits.
InterProiIPR001589. Actinin_actin-bd_CS.
IPR001715. CH-domain.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
[Graphical view]
PfamiPF00307. CH. 4 hits.
PF13499. EF-hand_7. 1 hit.
[Graphical view]
SMARTiSM00033. CH. 4 hits.
SM00054. EFh. 2 hits.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
SSF47576. SSF47576. 1 hit.
PROSITEiPS00019. ACTININ_1. 2 hits.
PS00020. ACTININ_2. 2 hits.
PS50021. CH. 4 hits.
PS00018. EF_HAND_1. 2 hits.
PS50222. EF_HAND_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q61233-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MARGSVSDEE MMELREAFAK VDTDGNGYIS CNELNDLFKA ACLPLPGYRV
60 70 80 90 100
REITENLMAT GDLDQDGKIS FDEFIKVFHG LKSTEVAKTF RKAINKKEGI
110 120 130 140 150
CAIGGTSEQS SVGTQHSYSE EEKYAFVNWI NKALENDPDC RHVIPMNPNT
160 170 180 190 200
DDLFNAVGDG IVLCKMINLS VPDTIDERTI NKKKLTPFTI QENLNLALNS
210 220 230 240 250
ASAIGCHVVN IGAEDLKEGK PYLVLGLLWQ VIKIGLFADI ELSRNEALIA
260 270 280 290 300
LLREGESLED LMKLSPEELL LRWANYHLEN AGCTKITNFS TDIKDSKAYY
310 320 330 340 350
HLLEQVAPKG DEEGIPAVVI DMSGLREKDD IQRAECMLQQ AERLGCRQFV
360 370 380 390 400
TATDVVRGNP KLNLAFIANL FNKYPALHKP ENQDIDWGAL EGETREERTF
410 420 430 440 450
RNWMNSLGVN PRVNHLYSDL SDALVIFQLY EKIKVPVDWN RVNKPPYPKL
460 470 480 490 500
GGNMKKLENC NYAVDLGKNQ AKFSLVGIAG QDLNEGNRTL TLALVWQLMR
510 520 530 540 550
RYTLNILEDI GGGQKVNDDI IVNWVNTTLK EAQKSSSIAS FKDPKISTSL
560 570 580 590 600
PVLDLIDAIQ PGSINYDLLK TENLDDEEKL NNAKYAISMA RKIGARVYAL
610 620
PEDLVEVNPK MVMTVFACLM GKGMKRV
Length:627
Mass (Da):70,149
Last modified:January 23, 2007 - v4
Checksum:iF0A1E6CA1C11A8E6
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti26N → K in BAC27208 (PubMed:16141072).Curated1
Sequence conflicti83S → T in BAA07085 (PubMed:7897227).Curated1
Sequence conflicti292D → H in BAA07085 (PubMed:7897227).Curated1
Sequence conflicti367 – 369IAN → ANL in BAA07085 (PubMed:7897227).Curated3
Sequence conflicti479A → G in BAA07085 (PubMed:7897227).Curated1
Sequence conflicti527T → M in BAA07085 (PubMed:7897227).Curated1
Sequence conflicti558A → S in BAB26141 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D37837 mRNA. Translation: BAA07085.1.
AK009211 mRNA. Translation: BAB26141.1.
AK030806 mRNA. Translation: BAE20456.1.
AK030987 mRNA. Translation: BAC27205.1.
AK030996 mRNA. Translation: BAC27208.1.
AK088202 mRNA. Translation: BAC40207.1.
AK149790 mRNA. Translation: BAE29087.1.
AK151155 mRNA. Translation: BAE30161.1.
AK152891 mRNA. Translation: BAE31574.1.
AK153082 mRNA. Translation: BAE31707.1.
AK153206 mRNA. Translation: BAE31806.1.
AK169728 mRNA. Translation: BAE41332.1.
AK169833 mRNA. Translation: BAE41398.1.
BC022943 mRNA. Translation: AAH22943.1.
CCDSiCCDS27276.1.
PIRiI49445.
RefSeqiNP_001234913.1. NM_001247984.1.
NP_032905.2. NM_008879.4.
XP_006518762.1. XM_006518699.1.
XP_006518764.1. XM_006518701.3.
XP_006518765.1. XM_006518702.3.
XP_006518766.1. XM_006518703.3.
XP_006518767.1. XM_006518704.1.
XP_017171409.1. XM_017315920.1.
UniGeneiMm.153911.
Mm.484415.

Genome annotation databases

EnsembliENSMUST00000124499; ENSMUSP00000121201; ENSMUSG00000021998.
ENSMUST00000131802; ENSMUSP00000117137; ENSMUSG00000021998.
ENSMUST00000145303; ENSMUSP00000116271; ENSMUSG00000021998.
GeneIDi18826.
KEGGimmu:18826.
UCSCiuc007uqm.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D37837 mRNA. Translation: BAA07085.1.
AK009211 mRNA. Translation: BAB26141.1.
AK030806 mRNA. Translation: BAE20456.1.
AK030987 mRNA. Translation: BAC27205.1.
AK030996 mRNA. Translation: BAC27208.1.
AK088202 mRNA. Translation: BAC40207.1.
AK149790 mRNA. Translation: BAE29087.1.
AK151155 mRNA. Translation: BAE30161.1.
AK152891 mRNA. Translation: BAE31574.1.
AK153082 mRNA. Translation: BAE31707.1.
AK153206 mRNA. Translation: BAE31806.1.
AK169728 mRNA. Translation: BAE41332.1.
AK169833 mRNA. Translation: BAE41398.1.
BC022943 mRNA. Translation: AAH22943.1.
CCDSiCCDS27276.1.
PIRiI49445.
RefSeqiNP_001234913.1. NM_001247984.1.
NP_032905.2. NM_008879.4.
XP_006518762.1. XM_006518699.1.
XP_006518764.1. XM_006518701.3.
XP_006518765.1. XM_006518702.3.
XP_006518766.1. XM_006518703.3.
XP_006518767.1. XM_006518704.1.
XP_017171409.1. XM_017315920.1.
UniGeneiMm.153911.
Mm.484415.

3D structure databases

ProteinModelPortaliQ61233.
SMRiQ61233.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi202256. 4 interactors.
IntActiQ61233. 7 interactors.
MINTiMINT-217293.
STRINGi10090.ENSMUSP00000116271.

PTM databases

iPTMnetiQ61233.
PhosphoSitePlusiQ61233.
SwissPalmiQ61233.

2D gel databases

COMPLUYEAST-2DPAGEQ61233.
REPRODUCTION-2DPAGEQ61233.

Proteomic databases

EPDiQ61233.
MaxQBiQ61233.
PaxDbiQ61233.
PeptideAtlasiQ61233.
PRIDEiQ61233.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000124499; ENSMUSP00000121201; ENSMUSG00000021998.
ENSMUST00000131802; ENSMUSP00000117137; ENSMUSG00000021998.
ENSMUST00000145303; ENSMUSP00000116271; ENSMUSG00000021998.
GeneIDi18826.
KEGGimmu:18826.
UCSCiuc007uqm.2. mouse.

Organism-specific databases

CTDi3936.
MGIiMGI:104808. Lcp1.

Phylogenomic databases

eggNOGiKOG0046. Eukaryota.
COG5069. LUCA.
GeneTreeiENSGT00390000005691.
HOGENOMiHOG000213447.
HOVERGENiHBG003082.
InParanoidiQ61233.
KOiK17276.
OMAiQPGSIKY.
OrthoDBiEOG091G03YP.
PhylomeDBiQ61233.
TreeFamiTF300680.

Miscellaneous databases

PROiQ61233.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000021998.
CleanExiMM_LCP1.
ExpressionAtlasiQ61233. baseline and differential.
GenevisibleiQ61233. MM.

Family and domain databases

CDDicd00014. CH. 2 hits.
cd00051. EFh. 1 hit.
Gene3Di1.10.238.10. 1 hit.
1.10.418.10. 4 hits.
InterProiIPR001589. Actinin_actin-bd_CS.
IPR001715. CH-domain.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
[Graphical view]
PfamiPF00307. CH. 4 hits.
PF13499. EF-hand_7. 1 hit.
[Graphical view]
SMARTiSM00033. CH. 4 hits.
SM00054. EFh. 2 hits.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
SSF47576. SSF47576. 1 hit.
PROSITEiPS00019. ACTININ_1. 2 hits.
PS00020. ACTININ_2. 2 hits.
PS50021. CH. 4 hits.
PS00018. EF_HAND_1. 2 hits.
PS50222. EF_HAND_2. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPLSL_MOUSE
AccessioniPrimary (citable) accession number: Q61233
Secondary accession number(s): Q3UE24, Q8R1X3, Q9CV77
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: January 23, 2007
Last modified: November 30, 2016
This is version 164 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.