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Protein

5-hydroxytryptamine receptor 1D

Gene

Htr1d

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

G-protein coupled receptor for 5-hydroxytryptamine (serotonin). Also functions as a receptor for various alkaloids and psychoactive substances. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors, such as adenylate cyclase. Signaling inhibits adenylate cyclase activity. Regulates the release of 5-hydroxytryptamine in the brain, and thereby affects neural activity. May also play a role in regulating the release of other neurotransmitters. May play a role in vasoconstriction.

GO - Molecular functioni

  • serotonin binding Source: MGI
  • serotonin receptor activity Source: MGI

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

G-protein coupled receptor, Receptor, Transducer

Enzyme and pathway databases

ReactomeiREACT_331048. G alpha (i) signalling events.
REACT_332615. Serotonin receptors.

Names & Taxonomyi

Protein namesi
Recommended name:
5-hydroxytryptamine receptor 1D
Short name:
5-HT-1D
Short name:
5-HT1D
Alternative name(s):
Serotonin receptor 1D
Gene namesi
Name:Htr1d
Synonyms:Gpcr14
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:96276. Htr1d.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 3535ExtracellularBy similarityAdd
BLAST
Transmembranei36 – 6126Helical; Name=1By similarityAdd
BLAST
Topological domaini62 – 7211CytoplasmicBy similarityAdd
BLAST
Transmembranei73 – 9523Helical; Name=2By similarityAdd
BLAST
Topological domaini96 – 10914ExtracellularBy similarityAdd
BLAST
Transmembranei110 – 13122Helical; Name=3By similarityAdd
BLAST
Topological domaini132 – 15120CytoplasmicBy similarityAdd
BLAST
Transmembranei152 – 17322Helical; Name=4By similarityAdd
BLAST
Topological domaini174 – 19118ExtracellularBy similarityAdd
BLAST
Transmembranei192 – 21423Helical; Name=5By similarityAdd
BLAST
Topological domaini215 – 29985CytoplasmicBy similarityAdd
BLAST
Transmembranei300 – 32324Helical; Name=6By similarityAdd
BLAST
Topological domaini324 – 3329ExtracellularBy similarity
Transmembranei333 – 35725Helical; Name=7By similarityAdd
BLAST
Topological domaini358 – 37417CytoplasmicBy similarityAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

No visible phenotype. Depending on the task, mutant mice show improved motor coordination, especially in avoiding hind limb slips when crossing a narrow beam and in climbing onto a horizontal hanging wire.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 3743745-hydroxytryptamine receptor 1DPRO_0000068928Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi5 – 51N-linked (GlcNAc...)Sequence Analysis
Glycosylationi17 – 171N-linked (GlcNAc...)Sequence Analysis
Glycosylationi21 – 211N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi108 ↔ 185PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PRIDEiQ61224.

PTM databases

PhosphoSiteiQ61224.

Expressioni

Tissue specificityi

Detected in the motor column in spinal cord, and in several cranial motor nuclei, including nucleus ambiguous, oculomotoris, trochelaris and abducens. Detected in gamma motor neurons in the lumbar spinal cord. Detected in proprioceptive sensory neurons in dorsal root ganglia.1 Publication

Gene expression databases

BgeeiQ61224.
CleanExiMM_HTR1D.
GenevestigatoriQ61224.

Interactioni

Subunit structurei

Homodimer. Heterodimer with HTR1B (By similarity).By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000112840.

Structurei

3D structure databases

ProteinModelPortaliQ61224.
SMRiQ61224. Positions 35-371.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni111 – 12010Agonist bindingBy similarity
Regioni311 – 3155Agonist bindingBy similarity

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi132 – 1343DRY motif; important for ligand-induced conformation changesBy similarity
Motifi349 – 3535NPxxY motif; important for ligand-induced conformation changes and signalingBy similarity

Sequence similaritiesi

Belongs to the G-protein coupled receptor 1 family.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG249628.
GeneTreeiENSGT00760000118795.
HOGENOMiHOG000239242.
HOVERGENiHBG106962.
InParanoidiQ61224.
KOiK04153.
OMAiCRDSCWI.
OrthoDBiEOG7NCV3Q.
TreeFamiTF316350.

Family and domain databases

InterProiIPR000505. 5HT1D_rcpt.
IPR002231. 5HT_rcpt.
IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
[Graphical view]
PANTHERiPTHR24247:SF17. PTHR24247:SF17. 1 hit.
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00514. 5HT1DRECEPTR.
PR01101. 5HTRECEPTOR.
PR00237. GPCRRHODOPSN.
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q61224-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSPPNQSLEG LPQEASNRSL NVTGAWDPEV LQALRISLVV VLSVITLATV
60 70 80 90 100
LSNAFVLTTI LLTKKLHTPA NYLIGSLATT DLLVSILVMP ISIAYTTTRT
110 120 130 140 150
WNFGQILCDI WVSSDITCCT ASILHLCVIA LDRYWAITDA LEYSKRRTAG
160 170 180 190 200
HAAAMIAAVW IISICISIPP LFWRQATAHE EMSDCLVNTS QISYTIYSTC
210 220 230 240 250
GAFYIPSILL IILYGRIYVA ARSRILNPPS LYGKRFTTAQ LITGSAGSSL
260 270 280 290 300
CSLNPSLHES HTHTVGSPLF FNQVKIKLAD SILERKRISA ARERKATKTL
310 320 330 340 350
GIILGAFIIC WLPFFVVSLV LPICRDSCWI HPALFDFFTW LGYLNSLINP
360 370
VIYTVFNEDF RQAFQKVVHF RKIS
Length:374
Mass (Da):41,594
Last modified:July 27, 2011 - v2
Checksum:i2FE947DF7DBEF14D
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti3 – 31P → L in CAA64395 (PubMed:8978753).Curated
Sequence conflicti22 – 221V → A in CAA64395 (PubMed:8978753).Curated
Sequence conflicti22 – 221V → A in EDL29938 (Ref. 4) Curated
Sequence conflicti22 – 221V → A in EDL29939 (Ref. 4) Curated
Sequence conflicti22 – 221V → A in AAI03533 (PubMed:15489334).Curated
Sequence conflicti22 – 221V → A in AAI03536 (PubMed:15489334).Curated
Sequence conflicti22 – 221V → A in AAI03537 (PubMed:15489334).Curated
Sequence conflicti373 – 3731I → A in CAA64395 (PubMed:8978753).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X94908 Genomic DNA. Translation: CAA64395.1.
AK082016 mRNA. Translation: BAC38393.1.
AL670673 Genomic DNA. Translation: CAM21107.1.
CH466552 Genomic DNA. Translation: EDL29938.1.
CH466552 Genomic DNA. Translation: EDL29939.1.
BC103532 mRNA. Translation: AAI03533.1.
BC103535 mRNA. Translation: AAI03536.1.
BC103536 mRNA. Translation: AAI03537.1.
L20335 mRNA. Translation: AAA16847.1.
CCDSiCCDS18806.1.
PIRiF48909.
RefSeqiNP_001272411.1. NM_001285482.1.
NP_001272412.1. NM_001285483.1.
NP_001272413.1. NM_001285484.1.
NP_032335.2. NM_008309.5.
XP_006538640.1. XM_006538577.2.
XP_006538641.1. XM_006538578.2.
UniGeneiMm.40573.

Genome annotation databases

EnsembliENSMUST00000088677; ENSMUSP00000086052; ENSMUSG00000070687.
ENSMUST00000117699; ENSMUSP00000112840; ENSMUSG00000070687.
ENSMUST00000121571; ENSMUSP00000112402; ENSMUSG00000070687.
GeneIDi15552.
KEGGimmu:15552.
UCSCiuc008vic.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X94908 Genomic DNA. Translation: CAA64395.1.
AK082016 mRNA. Translation: BAC38393.1.
AL670673 Genomic DNA. Translation: CAM21107.1.
CH466552 Genomic DNA. Translation: EDL29938.1.
CH466552 Genomic DNA. Translation: EDL29939.1.
BC103532 mRNA. Translation: AAI03533.1.
BC103535 mRNA. Translation: AAI03536.1.
BC103536 mRNA. Translation: AAI03537.1.
L20335 mRNA. Translation: AAA16847.1.
CCDSiCCDS18806.1.
PIRiF48909.
RefSeqiNP_001272411.1. NM_001285482.1.
NP_001272412.1. NM_001285483.1.
NP_001272413.1. NM_001285484.1.
NP_032335.2. NM_008309.5.
XP_006538640.1. XM_006538577.2.
XP_006538641.1. XM_006538578.2.
UniGeneiMm.40573.

3D structure databases

ProteinModelPortaliQ61224.
SMRiQ61224. Positions 35-371.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000112840.

Chemistry

ChEMBLiCHEMBL4297.
GuidetoPHARMACOLOGYi3.

Protein family/group databases

GPCRDBiSearch...

PTM databases

PhosphoSiteiQ61224.

Proteomic databases

PRIDEiQ61224.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000088677; ENSMUSP00000086052; ENSMUSG00000070687.
ENSMUST00000117699; ENSMUSP00000112840; ENSMUSG00000070687.
ENSMUST00000121571; ENSMUSP00000112402; ENSMUSG00000070687.
GeneIDi15552.
KEGGimmu:15552.
UCSCiuc008vic.2. mouse.

Organism-specific databases

CTDi3352.
MGIiMGI:96276. Htr1d.

Phylogenomic databases

eggNOGiNOG249628.
GeneTreeiENSGT00760000118795.
HOGENOMiHOG000239242.
HOVERGENiHBG106962.
InParanoidiQ61224.
KOiK04153.
OMAiCRDSCWI.
OrthoDBiEOG7NCV3Q.
TreeFamiTF316350.

Enzyme and pathway databases

ReactomeiREACT_331048. G alpha (i) signalling events.
REACT_332615. Serotonin receptors.

Miscellaneous databases

NextBioi288488.
PROiQ61224.
SOURCEiSearch...

Gene expression databases

BgeeiQ61224.
CleanExiMM_HTR1D.
GenevestigatoriQ61224.

Family and domain databases

InterProiIPR000505. 5HT1D_rcpt.
IPR002231. 5HT_rcpt.
IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
[Graphical view]
PANTHERiPTHR24247:SF17. PTHR24247:SF17. 1 hit.
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00514. 5HT1DRECEPTR.
PR01101. 5HTRECEPTOR.
PR00237. GPCRRHODOPSN.
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence and functional analysis of cloned guinea pig and rat serotonin 5-HT1D receptors: common pharmacological features within the 5-HT1D receptor subfamily."
    Wurch T., Palmier C., Colpaert F.C., Pauwels P.J.
    J. Neurochem. 68:410-418(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: NMRI.
    Tissue: Brain.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Head.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  4. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  6. "Identification, chromosomal location, and genome organization of mammalian G-protein-coupled receptors."
    Wilkie T.M., Chen Y., Gilbert D.J., Moore K.J., Yu L., Simon M.I., Copeland N.G., Jenkins N.A.
    Genomics 18:175-184(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 135-306.
    Tissue: Testis.
  7. "Sensorimotor function is modulated by the serotonin receptor 1d, a novel marker for gamma motor neurons."
    Enjin A., Leao K.E., Mikulovic S., Le Merre P., Tourtellotte W.G., Kullander K.
    Mol. Cell. Neurosci. 49:322-332(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISRUPTION PHENOTYPE, TISSUE SPECIFICITY.

Entry informationi

Entry namei5HT1D_MOUSE
AccessioniPrimary (citable) accession number: Q61224
Secondary accession number(s): Q3ZB48, Q61615, Q8BUW7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: July 27, 2011
Last modified: April 1, 2015
This is version 119 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.