Q61224 (5HT1D_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
5-hydroxytryptamine receptor 1D
Short name=5-HT-1D
Short name=5-HT1D

Alternative name(s):
Serotonin receptor 1D

Gene names

Name:	Htr1d
Synonyms:	Gpcr14

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	374 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	This is one of the several different receptors for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. The activity of this receptor is mediated by G proteins that inhibit adenylate cyclase activity.
Subcellular location	Cell membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the G-protein coupled receptor 1 family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	G-protein coupled receptor Receptor Transducer
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	response to toxin Inferred from direct assay. Source: MGI
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	serotonin binding Inferred from direct assay. Source: MGI serotonin receptor activity Inferred from direct assay. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 374	374	5-hydroxytryptamine receptor 1D		PRO_0000068928
Regions
	Topological domain	1 – 35	35	Extracellular By similarity
	Transmembrane	36 – 59	24	Helical; Name=1; By similarity
	Topological domain	60 – 72	13	Cytoplasmic By similarity
	Transmembrane	73 – 95	23	Helical; Name=2; By similarity
	Topological domain	96 – 105	10	Extracellular By similarity
	Transmembrane	106 – 131	26	Helical; Name=3; By similarity
	Topological domain	132 – 151	20	Cytoplasmic By similarity
	Transmembrane	152 – 173	22	Helical; Name=4; By similarity
	Topological domain	174 – 191	18	Extracellular By similarity
	Transmembrane	192 – 215	24	Helical; Name=5; By similarity
	Topological domain	216 – 299	84	Cytoplasmic By similarity
	Transmembrane	300 – 323	24	Helical; Name=6; By similarity
	Topological domain	324 – 332	9	Extracellular By similarity
	Transmembrane	333 – 357	25	Helical; Name=7; By similarity
	Topological domain	358 – 374	17	Cytoplasmic By similarity
Amino acid modifications
	Glycosylation	5	1	N-linked (GlcNAc...) Potential
	Glycosylation	17	1	N-linked (GlcNAc...) Potential
	Glycosylation	21	1	N-linked (GlcNAc...) Potential
	Disulfide bond	108 ↔ 185		By similarity
Experimental info
	Sequence conflict	3	1	P → L in CAA64395. Ref.1
	Sequence conflict	22	1	V → A in CAA64395. Ref.1
	Sequence conflict	373	1	I → A in CAA64395. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q61224 [UniParc]. Last modified July 27, 2011. Version 2. Checksum: 2FE947DF7DBEF14D	FASTA	374	41,594
	10 20 30 40 50 60 MSPPNQSLEG LPQEASNRSL NVTGAWDPEV LQALRISLVV VLSVITLATV LSNAFVLTTI 70 80 90 100 110 120 LLTKKLHTPA NYLIGSLATT DLLVSILVMP ISIAYTTTRT WNFGQILCDI WVSSDITCCT 130 140 150 160 170 180 ASILHLCVIA LDRYWAITDA LEYSKRRTAG HAAAMIAAVW IISICISIPP LFWRQATAHE 190 200 210 220 230 240 EMSDCLVNTS QISYTIYSTC GAFYIPSILL IILYGRIYVA ARSRILNPPS LYGKRFTTAQ 250 260 270 280 290 300 LITGSAGSSL CSLNPSLHES HTHTVGSPLF FNQVKIKLAD SILERKRISA ARERKATKTL 310 320 330 340 350 360 GIILGAFIIC WLPFFVVSLV LPICRDSCWI HPALFDFFTW LGYLNSLINP VIYTVFNEDF 370 RQAFQKVVHF RKIS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and functional analysis of cloned guinea pig and rat serotonin 5-HT1D receptors: common pharmacological features within the 5-HT1D receptor subfamily." Wurch T., Palmier C., Colpaert F.C., Pauwels P.J. J. Neurochem. 68:410-418(1997) [PubMed: 8978753] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: NMRI. Tissue: Brain.
[2]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Head.
[3]	"Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P. PLoS Biol. 7:E1000112-E1000112(2009) [PubMed: 19468303] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/6.
[4]	"Identification, chromosomal location, and genome organization of mammalian G-protein-coupled receptors." Wilkie T.M., Chen Y., Gilbert D.J., Moore K.J., Yu L., Simon M.I., Copeland N.G., Jenkins N.A. Genomics 18:175-184(1993) [PubMed: 8288218] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 135-306. Tissue: Testis.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X94908 Genomic DNA. Translation: CAA64395.1. AK082016 mRNA. Translation: BAC38393.1. AL670673 Genomic DNA. Translation: CAM21107.1. L20335 mRNA. Translation: AAA16847.1.
IPI	IPI00118873.
PIR	F48909.
RefSeq	NP_032335.2. NM_008309.4.
UniGene	Mm.40573.
3D structure databases
ProteinModelPortal	Q61224.
SMR	Q61224. Positions 31-370.
ModBase	Search...
Protein-protein interaction databases
STRING	Q61224.
Protein family/group databases
GPCRDB	Search...
Proteomic databases
PRIDE	Q61224.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000088677; ENSMUSP00000086052; ENSMUSG00000070687. ENSMUST00000117699; ENSMUSP00000112840; ENSMUSG00000070687. ENSMUST00000121571; ENSMUSP00000112402; ENSMUSG00000070687.
GeneID	15552.
KEGG	mmu:15552.
Organism-specific databases
CTD	3352.
MGI	MGI:96276. Htr1d.
Phylogenomic databases
GeneTree	ENSGT00590000082831.
HOGENOM	HBG445348.
HOVERGEN	HBG106962.
InParanoid	Q61224.
OrthoDB	EOG4R7VB6.
Gene expression databases
ArrayExpress	Q61224.
Bgee	Q61224.
CleanEx	MM_HTR1D.
Genevestigator	Q61224.
GermOnline	ENSMUSG00000070687. Mus musculus.
Family and domain databases
InterPro	IPR000505. 5HT1D_rcpt. IPR002231. 5HT_rcpt. IPR000276. 7TM_GPCR_Rhodpsn. IPR017452. GPCR_Rhodpsn_supfam. [Graphical view]
KO	K04153.
Pfam	PF00001. 7tm_1. 1 hit. [Graphical view]
PRINTS	PR00514. 5HT1DRECEPTR. PR01101. 5HTRECEPTOR. PR00237. GPCRRHODOPSN.
PROSITE	PS00237. G_PROTEIN_RECEP_F1_1. 1 hit. PS50262. G_PROTEIN_RECEP_F1_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	288488.
SOURCE	Search...

Entry information

Entry name

5HT1D_MOUSE

Accession

Primary (citable) accession number: Q61224
Secondary accession number(s): Q61615, Q8BUW7

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	July 27, 2011
Last modified:	December 14, 2011
This is version 93 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program