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Q61224 (5HT1D_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 113. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
5-hydroxytryptamine receptor 1D

Short name=5-HT-1D
Short name=5-HT1D
Alternative name(s):
Serotonin receptor 1D
Gene names
Name:Htr1d
Synonyms:Gpcr14
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length374 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

G-protein coupled receptor for 5-hydroxytryptamine (serotonin). Also functions as a receptor for various alkaloids and psychoactive substances. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors, such as adenylate cyclase. Signaling inhibits adenylate cyclase activity. Regulates the release of 5-hydroxytryptamine in the brain, and thereby affects neural activity. May also play a role in regulating the release of other neurotransmitters. May play a role in vasoconstriction.

Subunit structure

Homodimer. Heterodimer with HTR1B By similarity.

Subcellular location

Cell membrane; Multi-pass membrane protein.

Tissue specificity

Detected in the motor column in spinal cord, and in several cranial motor nuclei, including nucleus ambiguous, oculomotoris, trochelaris and abducens. Detected in gamma motor neurons in the lumbar spinal cord. Detected in proprioceptive sensory neurons in dorsal root ganglia. Ref.7

Disruption phenotype

No visible phenotype. Depending on the task, mutant mice show improved motor coordination, especially in avoiding hind limb slips when crossing a narrow beam and in climbing onto a horizontal hanging wire. Ref.7

Sequence similarities

Belongs to the G-protein coupled receptor 1 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 3743745-hydroxytryptamine receptor 1D
PRO_0000068928

Regions

Topological domain1 – 3535Extracellular By similarity
Transmembrane36 – 6126Helical; Name=1; By similarity
Topological domain62 – 7211Cytoplasmic By similarity
Transmembrane73 – 9523Helical; Name=2; By similarity
Topological domain96 – 10914Extracellular By similarity
Transmembrane110 – 13122Helical; Name=3; By similarity
Topological domain132 – 15120Cytoplasmic By similarity
Transmembrane152 – 17322Helical; Name=4; By similarity
Topological domain174 – 19118Extracellular By similarity
Transmembrane192 – 21423Helical; Name=5; By similarity
Topological domain215 – 29985Cytoplasmic By similarity
Transmembrane300 – 32324Helical; Name=6; By similarity
Topological domain324 – 3329Extracellular By similarity
Transmembrane333 – 35725Helical; Name=7; By similarity
Topological domain358 – 37417Cytoplasmic By similarity
Region111 – 12010Agonist binding By similarity
Region311 – 3155Agonist binding By similarity
Motif132 – 1343DRY motif; important for ligand-induced conformation changes By similarity
Motif349 – 3535NPxxY motif; important for ligand-induced conformation changes and signaling By similarity

Amino acid modifications

Glycosylation51N-linked (GlcNAc...) Potential
Glycosylation171N-linked (GlcNAc...) Potential
Glycosylation211N-linked (GlcNAc...) Potential
Disulfide bond108 ↔ 185 By similarity

Experimental info

Sequence conflict31P → L in CAA64395. Ref.1
Sequence conflict221V → A in CAA64395. Ref.1
Sequence conflict221V → A in EDL29938. Ref.4
Sequence conflict221V → A in EDL29939. Ref.4
Sequence conflict221V → A in AAI03533. Ref.5
Sequence conflict221V → A in AAI03536. Ref.5
Sequence conflict221V → A in AAI03537. Ref.5
Sequence conflict3731I → A in CAA64395. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q61224 [UniParc].

Last modified July 27, 2011. Version 2.
Checksum: 2FE947DF7DBEF14D

FASTA37441,594
        10         20         30         40         50         60 
MSPPNQSLEG LPQEASNRSL NVTGAWDPEV LQALRISLVV VLSVITLATV LSNAFVLTTI 

        70         80         90        100        110        120 
LLTKKLHTPA NYLIGSLATT DLLVSILVMP ISIAYTTTRT WNFGQILCDI WVSSDITCCT 

       130        140        150        160        170        180 
ASILHLCVIA LDRYWAITDA LEYSKRRTAG HAAAMIAAVW IISICISIPP LFWRQATAHE 

       190        200        210        220        230        240 
EMSDCLVNTS QISYTIYSTC GAFYIPSILL IILYGRIYVA ARSRILNPPS LYGKRFTTAQ 

       250        260        270        280        290        300 
LITGSAGSSL CSLNPSLHES HTHTVGSPLF FNQVKIKLAD SILERKRISA ARERKATKTL 

       310        320        330        340        350        360 
GIILGAFIIC WLPFFVVSLV LPICRDSCWI HPALFDFFTW LGYLNSLINP VIYTVFNEDF 

       370 
RQAFQKVVHF RKIS 

« Hide

References

« Hide 'large scale' references
[1]"Sequence and functional analysis of cloned guinea pig and rat serotonin 5-HT1D receptors: common pharmacological features within the 5-HT1D receptor subfamily."
Wurch T., Palmier C., Colpaert F.C., Pauwels P.J.
J. Neurochem. 68:410-418(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: NMRI.
Tissue: Brain.
[2]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6J.
Tissue: Head.
[3]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[4]Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[6]"Identification, chromosomal location, and genome organization of mammalian G-protein-coupled receptors."
Wilkie T.M., Chen Y., Gilbert D.J., Moore K.J., Yu L., Simon M.I., Copeland N.G., Jenkins N.A.
Genomics 18:175-184(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 135-306.
Tissue: Testis.
[7]"Sensorimotor function is modulated by the serotonin receptor 1d, a novel marker for gamma motor neurons."
Enjin A., Leao K.E., Mikulovic S., Le Merre P., Tourtellotte W.G., Kullander K.
Mol. Cell. Neurosci. 49:322-332(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: DISRUPTION PHENOTYPE, TISSUE SPECIFICITY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X94908 Genomic DNA. Translation: CAA64395.1.
AK082016 mRNA. Translation: BAC38393.1.
AL670673 Genomic DNA. Translation: CAM21107.1.
CH466552 Genomic DNA. Translation: EDL29938.1.
CH466552 Genomic DNA. Translation: EDL29939.1.
BC103532 mRNA. Translation: AAI03533.1.
BC103535 mRNA. Translation: AAI03536.1.
BC103536 mRNA. Translation: AAI03537.1.
L20335 mRNA. Translation: AAA16847.1.
CCDSCCDS18806.1.
PIRF48909.
RefSeqNP_001272411.1. NM_001285482.1.
NP_001272412.1. NM_001285483.1.
NP_001272413.1. NM_001285484.1.
NP_032335.2. NM_008309.5.
XP_006538639.1. XM_006538576.1.
XP_006538640.1. XM_006538577.1.
XP_006538641.1. XM_006538578.1.
UniGeneMm.40573.

3D structure databases

ProteinModelPortalQ61224.
SMRQ61224. Positions 35-371.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10090.ENSMUSP00000112840.

Chemistry

BindingDBQ61224.
ChEMBLCHEMBL4297.
GuidetoPHARMACOLOGY3.

Protein family/group databases

GPCRDBSearch...

PTM databases

PhosphoSiteQ61224.

Proteomic databases

PRIDEQ61224.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000088677; ENSMUSP00000086052; ENSMUSG00000070687.
ENSMUST00000117699; ENSMUSP00000112840; ENSMUSG00000070687.
ENSMUST00000121571; ENSMUSP00000112402; ENSMUSG00000070687.
GeneID15552.
KEGGmmu:15552.
UCSCuc008vic.2. mouse.

Organism-specific databases

CTD3352.
MGIMGI:96276. Htr1d.

Phylogenomic databases

eggNOGNOG249628.
GeneTreeENSGT00750000117301.
HOGENOMHOG000239242.
HOVERGENHBG106962.
InParanoidQ8BUW7.
KOK04153.
OMACRDSCWI.
OrthoDBEOG7NCV3Q.
TreeFamTF316350.

Gene expression databases

BgeeQ61224.
CleanExMM_HTR1D.
GenevestigatorQ61224.

Family and domain databases

Gene3D1.20.1070.10. 2 hits.
InterProIPR000505. 5HT1D_rcpt.
IPR002231. 5HT_rcpt.
IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
[Graphical view]
PANTHERPTHR24247:SF17. PTHR24247:SF17. 1 hit.
PfamPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSPR00514. 5HT1DRECEPTR.
PR01101. 5HTRECEPTOR.
PR00237. GPCRRHODOPSN.
PROSITEPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio288488.
PROQ61224.
SOURCESearch...

Entry information

Entry name5HT1D_MOUSE
AccessionPrimary (citable) accession number: Q61224
Secondary accession number(s): Q3ZB48, Q61615, Q8BUW7
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: July 27, 2011
Last modified: July 9, 2014
This is version 113 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

7-transmembrane G-linked receptors

List of 7-transmembrane G-linked receptor entries