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Q61216

- MRE11_MOUSE

UniProt

Q61216 - MRE11_MOUSE

Protein

Double-strand break repair protein MRE11A

Gene

Mre11a

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 116 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Component of the MRN complex, which plays a central role in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity and meiosis. The complex possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity, which are provided by MRE11A. RAD50 may be required to bind DNA ends and hold them in close proximity. This could facilitate searches for short or long regions of sequence homology in the recombining DNA templates, and may also stimulate the activity of DNA ligases and/or restrict the nuclease activity of MRE11A to prevent nucleolytic degradation past a given point. The complex may also be required for DNA damage signaling via activation of the ATM kinase. In telomeres the MRN complex may modulate t-loop formation By similarity.By similarity

    Cofactori

    Manganese.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei129 – 1291Proton donorBy similarity

    GO - Molecular functioni

    1. 3'-5' exonuclease activity Source: RefGenome
    2. ATP-dependent DNA helicase activity Source: Ensembl
    3. DNA binding Source: Ensembl
    4. endonuclease activity Source: RefGenome
    5. manganese ion binding Source: InterPro

    GO - Biological processi

    1. base-excision repair Source: RefGenome
    2. cell proliferation Source: MGI
    3. cellular response to DNA damage stimulus Source: MGI
    4. chromosome organization Source: MGI
    5. double-strand break repair Source: MGI
    6. intra-S DNA damage checkpoint Source: MGI
    7. mitotic G2 DNA damage checkpoint Source: MGI
    8. negative regulation of DNA endoreduplication Source: Ensembl
    9. nucleic acid phosphodiester bond hydrolysis Source: GOC
    10. nucleotide-excision repair Source: RefGenome
    11. positive regulation of kinase activity Source: Ensembl
    12. positive regulation of protein autophosphorylation Source: Ensembl
    13. sister chromatid cohesion Source: Ensembl
    14. synapsis Source: MGI
    15. telomere maintenance Source: RefGenome

    Keywords - Molecular functioni

    Endonuclease, Exonuclease, Hydrolase, Nuclease

    Keywords - Biological processi

    DNA damage, DNA repair, Meiosis

    Keywords - Ligandi

    Manganese

    Enzyme and pathway databases

    ReactomeiREACT_188819. DNA Damage/Telomere Stress Induced Senescence.
    REACT_196447. IRF3-mediated induction of type I IFN.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Double-strand break repair protein MRE11A
    Short name:
    MmMRE11A
    Alternative name(s):
    Meiotic recombination 11 homolog 1
    Short name:
    MRE11 homolog 1
    Meiotic recombination 11 homolog A
    Short name:
    MRE11 homolog A
    Gene namesi
    Name:Mre11a
    Synonyms:Mre11
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 9

    Organism-specific databases

    MGIiMGI:1100512. Mre11a.

    Subcellular locationi

    Nucleus By similarity
    Note: Localizes to discrete nuclear foci after treatment with genotoxic agents.By similarity

    GO - Cellular componenti

    1. chromatin Source: RefGenome
    2. chromosome, telomeric region Source: Ensembl
    3. Mre11 complex Source: RefGenome
    4. nucleus Source: MGI
    5. site of double-strand break Source: RefGenome

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 706705Double-strand break repair protein MRE11APRO_0000138674Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylserineBy similarity
    Modified residuei2 – 21Phosphoserine1 Publication
    Modified residuei648 – 6481PhosphoserineBy similarity
    Modified residuei686 – 6861PhosphoserineBy similarity
    Modified residuei699 – 6991PhosphoserineBy similarity

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    MaxQBiQ61216.
    PaxDbiQ61216.
    PRIDEiQ61216.

    PTM databases

    PhosphoSiteiQ61216.

    Miscellaneous databases

    PMAP-CutDBQ61216.

    Expressioni

    Gene expression databases

    ArrayExpressiQ61216.
    BgeeiQ61216.
    GenevestigatoriQ61216.

    Interactioni

    Subunit structurei

    Component of the MRN complex composed of two heterodimers RAD50/MRE11A associated with a single NBN. Component of the BASC complex, at least composed of BRCA1, MSH2, MSH6, MLH1, ATM, BLM, RAD50, MRE11A and NBN. Interacts with DCLRE1C/Artemis and DCLRE1B/Apollo By similarity. Interacts with ATF2 By similarity.By similarity

    Protein-protein interaction databases

    BioGridi201486. 4 interactions.
    DIPiDIP-46803N.
    IntActiQ61216. 4 interactions.

    Structurei

    3D structure databases

    ProteinModelPortaliQ61216.
    SMRiQ61216. Positions 8-400.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the MRE11/RAD32 family.Curated

    Phylogenomic databases

    eggNOGiCOG0420.
    GeneTreeiENSGT00390000017288.
    HOGENOMiHOG000216581.
    HOVERGENiHBG052508.
    InParanoidiQ61216.
    KOiK10865.
    OMAiQVTMLRP.
    OrthoDBiEOG7VB2F1.
    PhylomeDBiQ61216.
    TreeFamiTF101105.

    Family and domain databases

    Gene3Di3.60.21.10. 2 hits.
    InterProiIPR004843. Calcineurin-like_PHP_apaH.
    IPR003701. DNA_repair_Mre11.
    IPR029052. Metallo-depent_PP-like.
    IPR007281. Mre11_DNA-bd.
    [Graphical view]
    PANTHERiPTHR10139. PTHR10139. 1 hit.
    PfamiPF00149. Metallophos. 1 hit.
    PF04152. Mre11_DNA_bind. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000882. DSB_repair_MRE11. 1 hit.
    SUPFAMiSSF56300. SSF56300. 2 hits.
    TIGRFAMsiTIGR00583. mre11. 1 hit.

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q61216-1) [UniParc]FASTAAdd to Basket

    Also known as: A

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MSPTDPLDDE DTFKILVATD IHLGFMEKDA VRGNDTFVTF DEILRLALEN    50
    EVDFILLGGD LFHENKPSRK TLHSCLELLR KYCMGDRPVQ FEVISDQSVN 100
    FGFSKFPWVN YQDGNLNISI PVFSIHGNHD DPTGADALCA LDVLSCAGFV 150
    NHFGRSMSVE KVDISPVLLQ KGSTKLALYG LGSIPDERLY RMFVNKKVTM 200
    LRPKEDENSW FNLFVIHQNR SKHGNTNFIP EQFLDDFIDL VIWGHEHECK 250
    IGPIKNEQQL FYVSQPGSSV VTSLSPGEAV KKHVGLLRIK GRKMNMQKLP 300
    LRTVRRFFIE DVVLANHPNL FNPDNPKVTQ AIQSFCLEKI EEMLDSAERE 350
    RLGNPQQPGK PLIRLRVDYS GGFEPFNVLR FSQKFVDRVA NPKDVIHFFR 400
    HREQKGKTGE EINFGMLITK PASEGATLRV EDLVKQYFQT AEKNVQLSLL 450
    TERGMGEAVQ EFVDKEEKDA IEELVKYQLE KTQRFLKERH IDALEDKIDE 500
    EVRRFRESRQ RNTNEEDDEV REAMSRARAL RSQSETSTSA FSAEDLSFDT 550
    SEQTANDSDD SLSAVPSRGR GRGRGRRGAR GQSSAPRGGS QRGRDTGLEI 600
    TTRGRSSKAT SSTSRNMSII DAFRSTRQQP SRNVAPKNYS ETIEVDDSDE 650
    DDIFPTNSRA DQRWSGTTSS KRMSQSQTAK GVDFESDEDD DDDPFMSSSC 700
    PRRNRR 706
    Length:706
    Mass (Da):80,223
    Last modified:November 1, 1996 - v1
    Checksum:i0F12F51902FC179A
    GO
    Isoform 2 (identifier: Q61216-2) [UniParc]FASTAAdd to Basket

    Also known as: B

    The sequence of this isoform differs from the canonical sequence as follows:
         340-366: Missing.

    Show »
    Length:679
    Mass (Da):77,094
    Checksum:i3ACDE474E0425430
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei340 – 36627Missing in isoform 2. 1 PublicationVSP_003263Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U58987 mRNA. Translation: AAB04955.1.
    U60318 mRNA. Translation: AAB03664.1.
    BC065144 mRNA. Translation: AAH65144.1.
    CCDSiCCDS22827.1. [Q61216-1]
    RefSeqiNP_061206.1. NM_018736.2. [Q61216-1]
    XP_006510111.1. XM_006510048.1. [Q61216-1]
    XP_006510112.1. XM_006510049.1. [Q61216-2]
    XP_006510113.1. XM_006510050.1. [Q61216-2]
    UniGeneiMm.149071.

    Genome annotation databases

    EnsembliENSMUST00000034405; ENSMUSP00000034405; ENSMUSG00000031928. [Q61216-1]
    ENSMUST00000115632; ENSMUSP00000111295; ENSMUSG00000031928. [Q61216-2]
    GeneIDi17535.
    KEGGimmu:17535.
    UCSCiuc009ofc.1. mouse. [Q61216-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U58987 mRNA. Translation: AAB04955.1 .
    U60318 mRNA. Translation: AAB03664.1 .
    BC065144 mRNA. Translation: AAH65144.1 .
    CCDSi CCDS22827.1. [Q61216-1 ]
    RefSeqi NP_061206.1. NM_018736.2. [Q61216-1 ]
    XP_006510111.1. XM_006510048.1. [Q61216-1 ]
    XP_006510112.1. XM_006510049.1. [Q61216-2 ]
    XP_006510113.1. XM_006510050.1. [Q61216-2 ]
    UniGenei Mm.149071.

    3D structure databases

    ProteinModelPortali Q61216.
    SMRi Q61216. Positions 8-400.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 201486. 4 interactions.
    DIPi DIP-46803N.
    IntActi Q61216. 4 interactions.

    PTM databases

    PhosphoSitei Q61216.

    Proteomic databases

    MaxQBi Q61216.
    PaxDbi Q61216.
    PRIDEi Q61216.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000034405 ; ENSMUSP00000034405 ; ENSMUSG00000031928 . [Q61216-1 ]
    ENSMUST00000115632 ; ENSMUSP00000111295 ; ENSMUSG00000031928 . [Q61216-2 ]
    GeneIDi 17535.
    KEGGi mmu:17535.
    UCSCi uc009ofc.1. mouse. [Q61216-1 ]

    Organism-specific databases

    CTDi 4361.
    MGIi MGI:1100512. Mre11a.

    Phylogenomic databases

    eggNOGi COG0420.
    GeneTreei ENSGT00390000017288.
    HOGENOMi HOG000216581.
    HOVERGENi HBG052508.
    InParanoidi Q61216.
    KOi K10865.
    OMAi QVTMLRP.
    OrthoDBi EOG7VB2F1.
    PhylomeDBi Q61216.
    TreeFami TF101105.

    Enzyme and pathway databases

    Reactomei REACT_188819. DNA Damage/Telomere Stress Induced Senescence.
    REACT_196447. IRF3-mediated induction of type I IFN.

    Miscellaneous databases

    NextBioi 292160.
    PMAP-CutDB Q61216.
    PROi Q61216.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q61216.
    Bgeei Q61216.
    Genevestigatori Q61216.

    Family and domain databases

    Gene3Di 3.60.21.10. 2 hits.
    InterProi IPR004843. Calcineurin-like_PHP_apaH.
    IPR003701. DNA_repair_Mre11.
    IPR029052. Metallo-depent_PP-like.
    IPR007281. Mre11_DNA-bd.
    [Graphical view ]
    PANTHERi PTHR10139. PTHR10139. 1 hit.
    Pfami PF00149. Metallophos. 1 hit.
    PF04152. Mre11_DNA_bind. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000882. DSB_repair_MRE11. 1 hit.
    SUPFAMi SSF56300. SSF56300. 2 hits.
    TIGRFAMsi TIGR00583. mre11. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Oshiumi H., Shinohara A., Ogawa H.
      Submitted (MAY-1996) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2).
      Strain: CD-1.
      Tissue: Testis.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Strain: C57BL/6.
      Tissue: Retina.
    3. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Liver.

    Entry informationi

    Entry nameiMRE11_MOUSE
    AccessioniPrimary (citable) accession number: Q61216
    Secondary accession number(s): Q62430
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 26, 2001
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 116 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3