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Protein

Double-strand break repair protein MRE11A

Gene

Mre11a

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the MRN complex, which plays a central role in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity and meiosis. The complex possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity, which are provided by MRE11A. RAD50 may be required to bind DNA ends and hold them in close proximity. This could facilitate searches for short or long regions of sequence homology in the recombining DNA templates, and may also stimulate the activity of DNA ligases and/or restrict the nuclease activity of MRE11A to prevent nucleolytic degradation past a given point. The complex may also be required for DNA damage signaling via activation of the ATM kinase. In telomeres the MRN complex may modulate t-loop formation.By similarity

Cofactori

Mn2+By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei129 – 1291Proton donorBy similarity

GO - Molecular functioni

GO - Biological processi

  • cell proliferation Source: MGI
  • cellular response to DNA damage stimulus Source: MGI
  • chromosome organization Source: MGI
  • DNA duplex unwinding Source: MGI
  • double-strand break repair Source: MGI
  • double-strand break repair via nonhomologous end joining Source: MGI
  • heart development Source: Ensembl
  • intra-S DNA damage checkpoint Source: MGI
  • mitotic G2 DNA damage checkpoint Source: MGI
  • negative regulation of apoptotic process Source: MGI
  • negative regulation of DNA endoreduplication Source: MGI
  • negative regulation of viral entry into host cell Source: Ensembl
  • positive regulation of kinase activity Source: MGI
  • positive regulation of protein autophosphorylation Source: MGI
  • positive regulation of telomere maintenance Source: MGI
  • sister chromatid cohesion Source: MGI
  • synapsis Source: MGI
  • telomeric 3' overhang formation Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Exonuclease, Hydrolase, Nuclease

Keywords - Biological processi

DNA damage, DNA repair, Meiosis

Keywords - Ligandi

Manganese

Enzyme and pathway databases

ReactomeiR-MMU-1834949. Cytosolic sensors of pathogen-associated DNA.
R-MMU-2559586. DNA Damage/Telomere Stress Induced Senescence.
R-MMU-5685938. HDR through Single Strand Annealing (SSA).
R-MMU-5685939. HDR through MMEJ (alt-NHEJ).
R-MMU-5685942. HDR through Homologous Recombination (HRR).
R-MMU-5693548. Sensing of DNA Double Strand Breaks.
R-MMU-5693554. Resolution of D-loop Structures through Synthesis-Dependent Strand Annealing (SDSA).
R-MMU-5693565. Recruitment and ATM-mediated phosphorylation of repair and signaling proteins at DNA double strand breaks.
R-MMU-5693568. Resolution of D-loop Structures through Holliday Junction Intermediates.
R-MMU-5693571. Nonhomologous End-Joining (NHEJ).
R-MMU-5693579. Homologous DNA Pairing and Strand Exchange.
R-MMU-5693607. Processing of DNA double-strand break ends.
R-MMU-5693616. Presynaptic phase of homologous DNA pairing and strand exchange.
R-MMU-6804756. Regulation of TP53 Activity through Phosphorylation.
R-MMU-69473. G2/M DNA damage checkpoint.

Names & Taxonomyi

Protein namesi
Recommended name:
Double-strand break repair protein MRE11A
Short name:
MmMRE11A
Alternative name(s):
Meiotic recombination 11 homolog 1
Short name:
MRE11 homolog 1
Meiotic recombination 11 homolog A
Short name:
MRE11 homolog A
Gene namesi
Name:Mre11a
Synonyms:Mre11
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 9

Organism-specific databases

MGIiMGI:1100512. Mre11a.

Subcellular locationi

  • Nucleus By similarity
  • Chromosometelomere By similarity

  • Note: Localizes to discrete nuclear foci after treatment with genotoxic agents.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Chromosome, Nucleus, Telomere

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 706705Double-strand break repair protein MRE11APRO_0000138674Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserineBy similarity
Modified residuei2 – 21PhosphoserineCombined sources
Modified residuei275 – 2751PhosphoserineBy similarity
Modified residuei618 – 6181PhosphoserineBy similarity
Modified residuei640 – 6401PhosphoserineCombined sources
Modified residuei648 – 6481PhosphoserineCombined sources
Modified residuei676 – 6761PhosphoserineBy similarity
Modified residuei686 – 6861PhosphoserineCombined sources
Modified residuei699 – 6991PhosphoserineBy similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ61216.
MaxQBiQ61216.
PaxDbiQ61216.
PRIDEiQ61216.

PTM databases

iPTMnetiQ61216.
PhosphoSiteiQ61216.

Miscellaneous databases

PMAP-CutDBQ61216.

Expressioni

Gene expression databases

BgeeiENSMUSG00000031928.
ExpressionAtlasiQ61216. baseline and differential.
GenevisibleiQ61216. MM.

Interactioni

Subunit structurei

Component of the MRN complex composed of two heterodimers RAD50/MRE11A associated with a single NBN. Component of the BASC complex, at least composed of BRCA1, MSH2, MSH6, MLH1, ATM, BLM, RAD50, MRE11A and NBN. Found in a complex with TERF2. Interacts with DCLRE1C/Artemis and DCLRE1B/Apollo. Interacts with ATF2. Interacts with EXD2.By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi201486. 5 interactions.
DIPiDIP-46803N.
IntActiQ61216. 4 interactions.
STRINGi10090.ENSMUSP00000034405.

Structurei

3D structure databases

ProteinModelPortaliQ61216.
SMRiQ61216. Positions 8-400.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the MRE11/RAD32 family.Curated

Phylogenomic databases

eggNOGiKOG2310. Eukaryota.
COG0420. LUCA.
GeneTreeiENSGT00390000017288.
HOGENOMiHOG000216581.
HOVERGENiHBG052508.
InParanoidiQ61216.
KOiK10865.
OMAiNSRQPEK.
OrthoDBiEOG091G06P4.
PhylomeDBiQ61216.
TreeFamiTF101105.

Family and domain databases

Gene3Di3.60.21.10. 2 hits.
InterProiIPR004843. Calcineurin-like_PHP_apaH.
IPR029052. Metallo-depent_PP-like.
IPR003701. Mre11.
IPR007281. Mre11_DNA-bd.
[Graphical view]
PANTHERiPTHR10139:SF1. PTHR10139:SF1. 1 hit.
PfamiPF00149. Metallophos. 1 hit.
PF04152. Mre11_DNA_bind. 1 hit.
[Graphical view]
PIRSFiPIRSF000882. DSB_repair_MRE11. 1 hit.
SMARTiSM01347. Mre11_DNA_bind. 1 hit.
[Graphical view]
SUPFAMiSSF56300. SSF56300. 2 hits.
TIGRFAMsiTIGR00583. mre11. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q61216-1) [UniParc]FASTAAdd to basket
Also known as: A

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSPTDPLDDE DTFKILVATD IHLGFMEKDA VRGNDTFVTF DEILRLALEN
60 70 80 90 100
EVDFILLGGD LFHENKPSRK TLHSCLELLR KYCMGDRPVQ FEVISDQSVN
110 120 130 140 150
FGFSKFPWVN YQDGNLNISI PVFSIHGNHD DPTGADALCA LDVLSCAGFV
160 170 180 190 200
NHFGRSMSVE KVDISPVLLQ KGSTKLALYG LGSIPDERLY RMFVNKKVTM
210 220 230 240 250
LRPKEDENSW FNLFVIHQNR SKHGNTNFIP EQFLDDFIDL VIWGHEHECK
260 270 280 290 300
IGPIKNEQQL FYVSQPGSSV VTSLSPGEAV KKHVGLLRIK GRKMNMQKLP
310 320 330 340 350
LRTVRRFFIE DVVLANHPNL FNPDNPKVTQ AIQSFCLEKI EEMLDSAERE
360 370 380 390 400
RLGNPQQPGK PLIRLRVDYS GGFEPFNVLR FSQKFVDRVA NPKDVIHFFR
410 420 430 440 450
HREQKGKTGE EINFGMLITK PASEGATLRV EDLVKQYFQT AEKNVQLSLL
460 470 480 490 500
TERGMGEAVQ EFVDKEEKDA IEELVKYQLE KTQRFLKERH IDALEDKIDE
510 520 530 540 550
EVRRFRESRQ RNTNEEDDEV REAMSRARAL RSQSETSTSA FSAEDLSFDT
560 570 580 590 600
SEQTANDSDD SLSAVPSRGR GRGRGRRGAR GQSSAPRGGS QRGRDTGLEI
610 620 630 640 650
TTRGRSSKAT SSTSRNMSII DAFRSTRQQP SRNVAPKNYS ETIEVDDSDE
660 670 680 690 700
DDIFPTNSRA DQRWSGTTSS KRMSQSQTAK GVDFESDEDD DDDPFMSSSC

PRRNRR
Length:706
Mass (Da):80,223
Last modified:November 1, 1996 - v1
Checksum:i0F12F51902FC179A
GO
Isoform 2 (identifier: Q61216-2) [UniParc]FASTAAdd to basket
Also known as: B

The sequence of this isoform differs from the canonical sequence as follows:
     340-366: Missing.

Show »
Length:679
Mass (Da):77,094
Checksum:i3ACDE474E0425430
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei340 – 36627Missing in isoform 2. 1 PublicationVSP_003263Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U58987 mRNA. Translation: AAB04955.1.
U60318 mRNA. Translation: AAB03664.1.
BC065144 mRNA. Translation: AAH65144.1.
CCDSiCCDS22827.1. [Q61216-1]
CCDS80958.1. [Q61216-2]
RefSeqiNP_001297657.1. NM_001310728.1. [Q61216-2]
NP_061206.1. NM_018736.3. [Q61216-1]
XP_006510111.1. XM_006510048.2. [Q61216-1]
XP_006510112.1. XM_006510049.2. [Q61216-2]
UniGeneiMm.149071.

Genome annotation databases

EnsembliENSMUST00000034405; ENSMUSP00000034405; ENSMUSG00000031928. [Q61216-1]
ENSMUST00000115632; ENSMUSP00000111295; ENSMUSG00000031928. [Q61216-2]
GeneIDi17535.
KEGGimmu:17535.
UCSCiuc009ofc.1. mouse. [Q61216-1]
uc009ofd.1. mouse. [Q61216-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U58987 mRNA. Translation: AAB04955.1.
U60318 mRNA. Translation: AAB03664.1.
BC065144 mRNA. Translation: AAH65144.1.
CCDSiCCDS22827.1. [Q61216-1]
CCDS80958.1. [Q61216-2]
RefSeqiNP_001297657.1. NM_001310728.1. [Q61216-2]
NP_061206.1. NM_018736.3. [Q61216-1]
XP_006510111.1. XM_006510048.2. [Q61216-1]
XP_006510112.1. XM_006510049.2. [Q61216-2]
UniGeneiMm.149071.

3D structure databases

ProteinModelPortaliQ61216.
SMRiQ61216. Positions 8-400.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi201486. 5 interactions.
DIPiDIP-46803N.
IntActiQ61216. 4 interactions.
STRINGi10090.ENSMUSP00000034405.

PTM databases

iPTMnetiQ61216.
PhosphoSiteiQ61216.

Proteomic databases

EPDiQ61216.
MaxQBiQ61216.
PaxDbiQ61216.
PRIDEiQ61216.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000034405; ENSMUSP00000034405; ENSMUSG00000031928. [Q61216-1]
ENSMUST00000115632; ENSMUSP00000111295; ENSMUSG00000031928. [Q61216-2]
GeneIDi17535.
KEGGimmu:17535.
UCSCiuc009ofc.1. mouse. [Q61216-1]
uc009ofd.1. mouse. [Q61216-2]

Organism-specific databases

CTDi4361.
MGIiMGI:1100512. Mre11a.

Phylogenomic databases

eggNOGiKOG2310. Eukaryota.
COG0420. LUCA.
GeneTreeiENSGT00390000017288.
HOGENOMiHOG000216581.
HOVERGENiHBG052508.
InParanoidiQ61216.
KOiK10865.
OMAiNSRQPEK.
OrthoDBiEOG091G06P4.
PhylomeDBiQ61216.
TreeFamiTF101105.

Enzyme and pathway databases

ReactomeiR-MMU-1834949. Cytosolic sensors of pathogen-associated DNA.
R-MMU-2559586. DNA Damage/Telomere Stress Induced Senescence.
R-MMU-5685938. HDR through Single Strand Annealing (SSA).
R-MMU-5685939. HDR through MMEJ (alt-NHEJ).
R-MMU-5685942. HDR through Homologous Recombination (HRR).
R-MMU-5693548. Sensing of DNA Double Strand Breaks.
R-MMU-5693554. Resolution of D-loop Structures through Synthesis-Dependent Strand Annealing (SDSA).
R-MMU-5693565. Recruitment and ATM-mediated phosphorylation of repair and signaling proteins at DNA double strand breaks.
R-MMU-5693568. Resolution of D-loop Structures through Holliday Junction Intermediates.
R-MMU-5693571. Nonhomologous End-Joining (NHEJ).
R-MMU-5693579. Homologous DNA Pairing and Strand Exchange.
R-MMU-5693607. Processing of DNA double-strand break ends.
R-MMU-5693616. Presynaptic phase of homologous DNA pairing and strand exchange.
R-MMU-6804756. Regulation of TP53 Activity through Phosphorylation.
R-MMU-69473. G2/M DNA damage checkpoint.

Miscellaneous databases

PMAP-CutDBQ61216.
PROiQ61216.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000031928.
ExpressionAtlasiQ61216. baseline and differential.
GenevisibleiQ61216. MM.

Family and domain databases

Gene3Di3.60.21.10. 2 hits.
InterProiIPR004843. Calcineurin-like_PHP_apaH.
IPR029052. Metallo-depent_PP-like.
IPR003701. Mre11.
IPR007281. Mre11_DNA-bd.
[Graphical view]
PANTHERiPTHR10139:SF1. PTHR10139:SF1. 1 hit.
PfamiPF00149. Metallophos. 1 hit.
PF04152. Mre11_DNA_bind. 1 hit.
[Graphical view]
PIRSFiPIRSF000882. DSB_repair_MRE11. 1 hit.
SMARTiSM01347. Mre11_DNA_bind. 1 hit.
[Graphical view]
SUPFAMiSSF56300. SSF56300. 2 hits.
TIGRFAMsiTIGR00583. mre11. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMRE11_MOUSE
AccessioniPrimary (citable) accession number: Q61216
Secondary accession number(s): Q62430
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 26, 2001
Last sequence update: November 1, 1996
Last modified: September 7, 2016
This is version 136 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.