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Q61216

- MRE11_MOUSE

UniProt

Q61216 - MRE11_MOUSE

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Protein

Double-strand break repair protein MRE11A

Gene

Mre11a

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Component of the MRN complex, which plays a central role in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity and meiosis. The complex possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity, which are provided by MRE11A. RAD50 may be required to bind DNA ends and hold them in close proximity. This could facilitate searches for short or long regions of sequence homology in the recombining DNA templates, and may also stimulate the activity of DNA ligases and/or restrict the nuclease activity of MRE11A to prevent nucleolytic degradation past a given point. The complex may also be required for DNA damage signaling via activation of the ATM kinase. In telomeres the MRN complex may modulate t-loop formation (By similarity).By similarity

Cofactori

Mn2+By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei129 – 1291Proton donorBy similarity

GO - Molecular functioni

  1. 3'-5' exonuclease activity Source: RefGenome
  2. ATP-dependent DNA helicase activity Source: Ensembl
  3. DNA binding Source: Ensembl
  4. endonuclease activity Source: RefGenome
  5. manganese ion binding Source: InterPro

GO - Biological processi

  1. base-excision repair Source: RefGenome
  2. cell proliferation Source: MGI
  3. cellular response to DNA damage stimulus Source: MGI
  4. chromosome organization Source: MGI
  5. double-strand break repair Source: MGI
  6. intra-S DNA damage checkpoint Source: MGI
  7. mitotic G2 DNA damage checkpoint Source: MGI
  8. negative regulation of DNA endoreduplication Source: Ensembl
  9. nucleic acid phosphodiester bond hydrolysis Source: GOC
  10. nucleotide-excision repair Source: RefGenome
  11. positive regulation of kinase activity Source: Ensembl
  12. positive regulation of protein autophosphorylation Source: Ensembl
  13. sister chromatid cohesion Source: Ensembl
  14. synapsis Source: MGI
  15. telomere maintenance Source: RefGenome
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Exonuclease, Hydrolase, Nuclease

Keywords - Biological processi

DNA damage, DNA repair, Meiosis

Keywords - Ligandi

Manganese

Enzyme and pathway databases

ReactomeiREACT_188819. DNA Damage/Telomere Stress Induced Senescence.
REACT_196447. IRF3-mediated induction of type I IFN.
REACT_256555. Assembly of the RAD50-MRE11-NBS1 complex at DNA double-strand breaks.

Names & Taxonomyi

Protein namesi
Recommended name:
Double-strand break repair protein MRE11A
Short name:
MmMRE11A
Alternative name(s):
Meiotic recombination 11 homolog 1
Short name:
MRE11 homolog 1
Meiotic recombination 11 homolog A
Short name:
MRE11 homolog A
Gene namesi
Name:Mre11a
Synonyms:Mre11
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 9

Organism-specific databases

MGIiMGI:1100512. Mre11a.

Subcellular locationi

Nucleus By similarity
Note: Localizes to discrete nuclear foci after treatment with genotoxic agents.By similarity

GO - Cellular componenti

  1. chromatin Source: RefGenome
  2. chromosome, telomeric region Source: Ensembl
  3. Mre11 complex Source: RefGenome
  4. nucleus Source: MGI
  5. site of double-strand break Source: RefGenome
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 706705Double-strand break repair protein MRE11APRO_0000138674Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserineBy similarity
Modified residuei2 – 21Phosphoserine1 Publication
Modified residuei648 – 6481PhosphoserineBy similarity
Modified residuei686 – 6861PhosphoserineBy similarity
Modified residuei699 – 6991PhosphoserineBy similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiQ61216.
PaxDbiQ61216.
PRIDEiQ61216.

PTM databases

PhosphoSiteiQ61216.

Miscellaneous databases

PMAP-CutDBQ61216.

Expressioni

Gene expression databases

BgeeiQ61216.
ExpressionAtlasiQ61216. baseline and differential.
GenevestigatoriQ61216.

Interactioni

Subunit structurei

Component of the MRN complex composed of two heterodimers RAD50/MRE11A associated with a single NBN. Component of the BASC complex, at least composed of BRCA1, MSH2, MSH6, MLH1, ATM, BLM, RAD50, MRE11A and NBN. Interacts with DCLRE1C/Artemis and DCLRE1B/Apollo (By similarity). Interacts with ATF2 (By similarity).By similarity

Protein-protein interaction databases

BioGridi201486. 4 interactions.
DIPiDIP-46803N.
IntActiQ61216. 4 interactions.

Structurei

3D structure databases

ProteinModelPortaliQ61216.
SMRiQ61216. Positions 8-400.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the MRE11/RAD32 family.Curated

Phylogenomic databases

eggNOGiCOG0420.
GeneTreeiENSGT00390000017288.
HOGENOMiHOG000216581.
HOVERGENiHBG052508.
InParanoidiQ61216.
KOiK10865.
OMAiQVTMLRP.
OrthoDBiEOG7VB2F1.
PhylomeDBiQ61216.
TreeFamiTF101105.

Family and domain databases

Gene3Di3.60.21.10. 2 hits.
InterProiIPR004843. Calcineurin-like_PHP_apaH.
IPR003701. DNA_repair_Mre11.
IPR029052. Metallo-depent_PP-like.
IPR007281. Mre11_DNA-bd.
[Graphical view]
PANTHERiPTHR10139. PTHR10139. 1 hit.
PfamiPF00149. Metallophos. 1 hit.
PF04152. Mre11_DNA_bind. 1 hit.
[Graphical view]
PIRSFiPIRSF000882. DSB_repair_MRE11. 1 hit.
SUPFAMiSSF56300. SSF56300. 2 hits.
TIGRFAMsiTIGR00583. mre11. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q61216-1) [UniParc]FASTAAdd to Basket

Also known as: A

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSPTDPLDDE DTFKILVATD IHLGFMEKDA VRGNDTFVTF DEILRLALEN
60 70 80 90 100
EVDFILLGGD LFHENKPSRK TLHSCLELLR KYCMGDRPVQ FEVISDQSVN
110 120 130 140 150
FGFSKFPWVN YQDGNLNISI PVFSIHGNHD DPTGADALCA LDVLSCAGFV
160 170 180 190 200
NHFGRSMSVE KVDISPVLLQ KGSTKLALYG LGSIPDERLY RMFVNKKVTM
210 220 230 240 250
LRPKEDENSW FNLFVIHQNR SKHGNTNFIP EQFLDDFIDL VIWGHEHECK
260 270 280 290 300
IGPIKNEQQL FYVSQPGSSV VTSLSPGEAV KKHVGLLRIK GRKMNMQKLP
310 320 330 340 350
LRTVRRFFIE DVVLANHPNL FNPDNPKVTQ AIQSFCLEKI EEMLDSAERE
360 370 380 390 400
RLGNPQQPGK PLIRLRVDYS GGFEPFNVLR FSQKFVDRVA NPKDVIHFFR
410 420 430 440 450
HREQKGKTGE EINFGMLITK PASEGATLRV EDLVKQYFQT AEKNVQLSLL
460 470 480 490 500
TERGMGEAVQ EFVDKEEKDA IEELVKYQLE KTQRFLKERH IDALEDKIDE
510 520 530 540 550
EVRRFRESRQ RNTNEEDDEV REAMSRARAL RSQSETSTSA FSAEDLSFDT
560 570 580 590 600
SEQTANDSDD SLSAVPSRGR GRGRGRRGAR GQSSAPRGGS QRGRDTGLEI
610 620 630 640 650
TTRGRSSKAT SSTSRNMSII DAFRSTRQQP SRNVAPKNYS ETIEVDDSDE
660 670 680 690 700
DDIFPTNSRA DQRWSGTTSS KRMSQSQTAK GVDFESDEDD DDDPFMSSSC

PRRNRR
Length:706
Mass (Da):80,223
Last modified:November 1, 1996 - v1
Checksum:i0F12F51902FC179A
GO
Isoform 2 (identifier: Q61216-2) [UniParc]FASTAAdd to Basket

Also known as: B

The sequence of this isoform differs from the canonical sequence as follows:
     340-366: Missing.

Show »
Length:679
Mass (Da):77,094
Checksum:i3ACDE474E0425430
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei340 – 36627Missing in isoform 2. 1 PublicationVSP_003263Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U58987 mRNA. Translation: AAB04955.1.
U60318 mRNA. Translation: AAB03664.1.
BC065144 mRNA. Translation: AAH65144.1.
CCDSiCCDS22827.1. [Q61216-1]
RefSeqiNP_061206.1. NM_018736.2. [Q61216-1]
XP_006510111.1. XM_006510048.1. [Q61216-1]
XP_006510112.1. XM_006510049.1. [Q61216-2]
XP_006510113.1. XM_006510050.1. [Q61216-2]
UniGeneiMm.149071.

Genome annotation databases

EnsembliENSMUST00000034405; ENSMUSP00000034405; ENSMUSG00000031928. [Q61216-1]
ENSMUST00000115632; ENSMUSP00000111295; ENSMUSG00000031928. [Q61216-2]
GeneIDi17535.
KEGGimmu:17535.
UCSCiuc009ofc.1. mouse. [Q61216-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U58987 mRNA. Translation: AAB04955.1 .
U60318 mRNA. Translation: AAB03664.1 .
BC065144 mRNA. Translation: AAH65144.1 .
CCDSi CCDS22827.1. [Q61216-1 ]
RefSeqi NP_061206.1. NM_018736.2. [Q61216-1 ]
XP_006510111.1. XM_006510048.1. [Q61216-1 ]
XP_006510112.1. XM_006510049.1. [Q61216-2 ]
XP_006510113.1. XM_006510050.1. [Q61216-2 ]
UniGenei Mm.149071.

3D structure databases

ProteinModelPortali Q61216.
SMRi Q61216. Positions 8-400.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 201486. 4 interactions.
DIPi DIP-46803N.
IntActi Q61216. 4 interactions.

PTM databases

PhosphoSitei Q61216.

Proteomic databases

MaxQBi Q61216.
PaxDbi Q61216.
PRIDEi Q61216.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000034405 ; ENSMUSP00000034405 ; ENSMUSG00000031928 . [Q61216-1 ]
ENSMUST00000115632 ; ENSMUSP00000111295 ; ENSMUSG00000031928 . [Q61216-2 ]
GeneIDi 17535.
KEGGi mmu:17535.
UCSCi uc009ofc.1. mouse. [Q61216-1 ]

Organism-specific databases

CTDi 4361.
MGIi MGI:1100512. Mre11a.

Phylogenomic databases

eggNOGi COG0420.
GeneTreei ENSGT00390000017288.
HOGENOMi HOG000216581.
HOVERGENi HBG052508.
InParanoidi Q61216.
KOi K10865.
OMAi QVTMLRP.
OrthoDBi EOG7VB2F1.
PhylomeDBi Q61216.
TreeFami TF101105.

Enzyme and pathway databases

Reactomei REACT_188819. DNA Damage/Telomere Stress Induced Senescence.
REACT_196447. IRF3-mediated induction of type I IFN.
REACT_256555. Assembly of the RAD50-MRE11-NBS1 complex at DNA double-strand breaks.

Miscellaneous databases

NextBioi 292160.
PMAP-CutDB Q61216.
PROi Q61216.
SOURCEi Search...

Gene expression databases

Bgeei Q61216.
ExpressionAtlasi Q61216. baseline and differential.
Genevestigatori Q61216.

Family and domain databases

Gene3Di 3.60.21.10. 2 hits.
InterProi IPR004843. Calcineurin-like_PHP_apaH.
IPR003701. DNA_repair_Mre11.
IPR029052. Metallo-depent_PP-like.
IPR007281. Mre11_DNA-bd.
[Graphical view ]
PANTHERi PTHR10139. PTHR10139. 1 hit.
Pfami PF00149. Metallophos. 1 hit.
PF04152. Mre11_DNA_bind. 1 hit.
[Graphical view ]
PIRSFi PIRSF000882. DSB_repair_MRE11. 1 hit.
SUPFAMi SSF56300. SSF56300. 2 hits.
TIGRFAMsi TIGR00583. mre11. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Oshiumi H., Shinohara A., Ogawa H.
    Submitted (MAY-1996) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2).
    Strain: CD-1.
    Tissue: Testis.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: C57BL/6.
    Tissue: Retina.
  3. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.

Entry informationi

Entry nameiMRE11_MOUSE
AccessioniPrimary (citable) accession number: Q61216
Secondary accession number(s): Q62430
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 26, 2001
Last sequence update: November 1, 1996
Last modified: November 26, 2014
This is version 118 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3