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Q61216 (MRE11_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 114. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Double-strand break repair protein MRE11A

Short name=MmMRE11A
Alternative name(s):
Meiotic recombination 11 homolog 1
Short name=MRE11 homolog 1
Meiotic recombination 11 homolog A
Short name=MRE11 homolog A
Gene names
Name:Mre11a
Synonyms:Mre11
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length706 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Component of the MRN complex, which plays a central role in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity and meiosis. The complex possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity, which are provided by MRE11A. RAD50 may be required to bind DNA ends and hold them in close proximity. This could facilitate searches for short or long regions of sequence homology in the recombining DNA templates, and may also stimulate the activity of DNA ligases and/or restrict the nuclease activity of MRE11A to prevent nucleolytic degradation past a given point. The complex may also be required for DNA damage signaling via activation of the ATM kinase. In telomeres the MRN complex may modulate t-loop formation By similarity.

Cofactor

Manganese By similarity.

Subunit structure

Component of the MRN complex composed of two heterodimers RAD50/MRE11A associated with a single NBN. Component of the BASC complex, at least composed of BRCA1, MSH2, MSH6, MLH1, ATM, BLM, RAD50, MRE11A and NBN. Interacts with DCLRE1C/Artemis and DCLRE1B/Apollo By similarity. Interacts with ATF2 By similarity.

Subcellular location

Nucleus By similarity. Note: Localizes to discrete nuclear foci after treatment with genotoxic agents By similarity.

Sequence similarities

Belongs to the MRE11/RAD32 family.

Ontologies

Keywords
   Biological processDNA damage
DNA repair
Meiosis
   Cellular componentNucleus
   Coding sequence diversityAlternative splicing
   LigandManganese
   Molecular functionEndonuclease
Exonuclease
Hydrolase
Nuclease
   PTMAcetylation
Phosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcell proliferation

Inferred from mutant phenotype PubMed 9224597. Source: MGI

cellular response to DNA damage stimulus

Inferred from sequence orthology PubMed 17500065. Source: MGI

chromosome organization

Inferred from mutant phenotype PubMed 14690604. Source: MGI

double-strand break repair

Inferred from mutant phenotype PubMed 17291760. Source: MGI

intra-S DNA damage checkpoint

Inferred from mutant phenotype PubMed 14690604PubMed 18614044. Source: MGI

mitotic G2 DNA damage checkpoint

Inferred from mutant phenotype PubMed 14690604. Source: MGI

negative regulation of DNA endoreduplication

Inferred from electronic annotation. Source: Ensembl

positive regulation of kinase activity

Inferred from electronic annotation. Source: Ensembl

positive regulation of protein autophosphorylation

Inferred from electronic annotation. Source: Ensembl

sister chromatid cohesion

Inferred from electronic annotation. Source: Ensembl

synapsis

Inferred from mutant phenotype PubMed 17291760. Source: MGI

   Cellular_componentchromosome, telomeric region

Inferred from electronic annotation. Source: Ensembl

nucleus

Inferred from direct assay PubMed 12687013PubMed 15364958. Source: MGI

site of double-strand break

Inferred from electronic annotation. Source: Ensembl

   Molecular_functionATP-dependent DNA helicase activity

Inferred from electronic annotation. Source: Ensembl

DNA binding

Inferred from electronic annotation. Source: Ensembl

endonuclease activity

Inferred from electronic annotation. Source: UniProtKB-KW

exonuclease activity

Inferred from electronic annotation. Source: UniProtKB-KW

manganese ion binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q61216-1)

Also known as: A;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q61216-2)

Also known as: B;

The sequence of this isoform differs from the canonical sequence as follows:
     340-366: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 706705Double-strand break repair protein MRE11A
PRO_0000138674

Sites

Active site1291Proton donor By similarity

Amino acid modifications

Modified residue21N-acetylserine By similarity
Modified residue21Phosphoserine Ref.3
Modified residue6481Phosphoserine By similarity
Modified residue6861Phosphoserine By similarity
Modified residue6991Phosphoserine By similarity

Natural variations

Alternative sequence340 – 36627Missing in isoform 2.
VSP_003263

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 (A) [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 0F12F51902FC179A

FASTA70680,223
        10         20         30         40         50         60 
MSPTDPLDDE DTFKILVATD IHLGFMEKDA VRGNDTFVTF DEILRLALEN EVDFILLGGD 

        70         80         90        100        110        120 
LFHENKPSRK TLHSCLELLR KYCMGDRPVQ FEVISDQSVN FGFSKFPWVN YQDGNLNISI 

       130        140        150        160        170        180 
PVFSIHGNHD DPTGADALCA LDVLSCAGFV NHFGRSMSVE KVDISPVLLQ KGSTKLALYG 

       190        200        210        220        230        240 
LGSIPDERLY RMFVNKKVTM LRPKEDENSW FNLFVIHQNR SKHGNTNFIP EQFLDDFIDL 

       250        260        270        280        290        300 
VIWGHEHECK IGPIKNEQQL FYVSQPGSSV VTSLSPGEAV KKHVGLLRIK GRKMNMQKLP 

       310        320        330        340        350        360 
LRTVRRFFIE DVVLANHPNL FNPDNPKVTQ AIQSFCLEKI EEMLDSAERE RLGNPQQPGK 

       370        380        390        400        410        420 
PLIRLRVDYS GGFEPFNVLR FSQKFVDRVA NPKDVIHFFR HREQKGKTGE EINFGMLITK 

       430        440        450        460        470        480 
PASEGATLRV EDLVKQYFQT AEKNVQLSLL TERGMGEAVQ EFVDKEEKDA IEELVKYQLE 

       490        500        510        520        530        540 
KTQRFLKERH IDALEDKIDE EVRRFRESRQ RNTNEEDDEV REAMSRARAL RSQSETSTSA 

       550        560        570        580        590        600 
FSAEDLSFDT SEQTANDSDD SLSAVPSRGR GRGRGRRGAR GQSSAPRGGS QRGRDTGLEI 

       610        620        630        640        650        660 
TTRGRSSKAT SSTSRNMSII DAFRSTRQQP SRNVAPKNYS ETIEVDDSDE DDIFPTNSRA 

       670        680        690        700 
DQRWSGTTSS KRMSQSQTAK GVDFESDEDD DDDPFMSSSC PRRNRR 

« Hide

Isoform 2 (B) [UniParc].

Checksum: 3ACDE474E0425430
Show »

FASTA67977,094

References

« Hide 'large scale' references
[1]Oshiumi H., Shinohara A., Ogawa H.
Submitted (MAY-1996) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2).
Strain: CD-1.
Tissue: Testis.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Strain: C57BL/6.
Tissue: Retina.
[3]"Large-scale phosphorylation analysis of mouse liver."
Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Liver.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U58987 mRNA. Translation: AAB04955.1.
U60318 mRNA. Translation: AAB03664.1.
BC065144 mRNA. Translation: AAH65144.1.
CCDSCCDS22827.1. [Q61216-1]
RefSeqNP_061206.1. NM_018736.2. [Q61216-1]
XP_006510111.1. XM_006510048.1. [Q61216-1]
XP_006510112.1. XM_006510049.1. [Q61216-2]
XP_006510113.1. XM_006510050.1. [Q61216-2]
UniGeneMm.149071.

3D structure databases

ProteinModelPortalQ61216.
SMRQ61216. Positions 8-400.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid201486. 4 interactions.
DIPDIP-46803N.
IntActQ61216. 3 interactions.

PTM databases

PhosphoSiteQ61216.

Proteomic databases

MaxQBQ61216.
PaxDbQ61216.
PRIDEQ61216.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000034405; ENSMUSP00000034405; ENSMUSG00000031928. [Q61216-1]
ENSMUST00000115632; ENSMUSP00000111295; ENSMUSG00000031928. [Q61216-2]
GeneID17535.
KEGGmmu:17535.
UCSCuc009ofc.1. mouse. [Q61216-1]

Organism-specific databases

CTD4361.
MGIMGI:1100512. Mre11a.

Phylogenomic databases

eggNOGCOG0420.
GeneTreeENSGT00390000017288.
HOGENOMHOG000216581.
HOVERGENHBG052508.
InParanoidQ61216.
KOK10865.
OMAQVTMLRP.
OrthoDBEOG7VB2F1.
PhylomeDBQ61216.
TreeFamTF101105.

Gene expression databases

ArrayExpressQ61216.
BgeeQ61216.
GenevestigatorQ61216.

Family and domain databases

Gene3D3.60.21.10. 2 hits.
InterProIPR004843. Calcineurin-like_PHP_apaH.
IPR003701. DNA_repair_Mre11.
IPR029052. Metallo-depent_PP-like.
IPR007281. Mre11_DNA-bd.
[Graphical view]
PANTHERPTHR10139. PTHR10139. 1 hit.
PfamPF00149. Metallophos. 1 hit.
PF04152. Mre11_DNA_bind. 1 hit.
[Graphical view]
PIRSFPIRSF000882. DSB_repair_MRE11. 1 hit.
SUPFAMSSF56300. SSF56300. 2 hits.
TIGRFAMsTIGR00583. mre11. 1 hit.
ProtoNetSearch...

Other

NextBio292160.
PMAP-CutDBQ61216.
PROQ61216.
SOURCESearch...

Entry information

Entry nameMRE11_MOUSE
AccessionPrimary (citable) accession number: Q61216
Secondary accession number(s): Q62430
Entry history
Integrated into UniProtKB/Swiss-Prot: September 26, 2001
Last sequence update: November 1, 1996
Last modified: July 9, 2014
This is version 114 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot