Q61216 (MRE11_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Double-strand break repair protein MRE11A
Short name=MmMRE11A

Alternative name(s):
Meiotic recombination 11 homolog 1
Short name=MRE11 homolog 1
Meiotic recombination 11 homolog A
Short name=MRE11 homolog A

Gene names

Name:	Mre11a
Synonyms:	Mre11

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	706 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Component of the MRN complex, which plays a central role in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity and meiosis. The complex possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity, which are provided by MRE11A. RAD50 may be required to bind DNA ends and hold them in close proximity. This could facilitate searches for short or long regions of sequence homology in the recombining DNA templates, and may also stimulate the activity of DNA ligases and/or restrict the nuclease activity of MRE11A to prevent nucleolytic degradation past a given point. The complex may also be required for DNA damage signaling via activation of the ATM kinase. In telomeres the MRN complex may modulate t-loop formation By similarity.
Cofactor	Manganese By similarity.
Subunit structure	Component of the MRN complex composed of two heterodimers RAD50/MRE11A associated with a single NBN. Component of the BASC complex, at least composed of BRCA1, MSH2, MSH6, MLH1, ATM, BLM, RAD50, MRE11A and NBN. Interacts with DCLRE1C/Artemis and DCLRE1B/Apollo By similarity.
Subcellular location	Nucleus By similarity. Note: Localizes to discrete nuclear foci after treatment with genotoxic agents By similarity.
Sequence similarities	Belongs to the MRE11/RAD32 family.

Ontologies

Keywords
Biological process	DNA damage DNA repair Meiosis
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing
Ligand	Manganese
Molecular function	Endonuclease Exonuclease Hydrolase Nuclease
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell cycle checkpoint Inferred from mutant phenotype PubMed 14690604PubMed 18614044. Source: MGI cell proliferation Inferred from mutant phenotype PubMed 9224597. Source: MGI double-strand break repair Inferred from mutant phenotype PubMed 17291760. Source: MGI negative regulation of DNA endoreduplication Inferred from electronic annotation. Source: Compara nucleic acid phosphodiester bond hydrolysis Inferred from electronic annotation. Source: GOC positive regulation of kinase activity Inferred from electronic annotation. Source: Compara positive regulation of protein autophosphorylation Inferred from electronic annotation. Source: Compara sister chromatid cohesion Inferred from electronic annotation. Source: Compara synapsis Inferred from mutant phenotype PubMed 17291760. Source: MGI
Cellular_component	chromosome, telomeric region Inferred from electronic annotation. Source: Compara nucleus Inferred from direct assay PubMed 12687013PubMed 15364958. Source: MGI
Molecular_function	ATP-dependent DNA helicase activity Inferred from electronic annotation. Source: Compara DNA binding Inferred from electronic annotation. Source: Compara endonuclease activity Inferred from electronic annotation. Source: UniProtKB-KW exonuclease activity Inferred from electronic annotation. Source: UniProtKB-KW manganese ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q61216-1)

Also known as: A;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q61216-2)

Also known as: B;

The sequence of this isoform differs from the canonical sequence as follows:
     340-366: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 706	706	Double-strand break repair protein MRE11A		PRO_0000138674
Sites
	Active site	129	1	Proton donor By similarity
Amino acid modifications
	Modified residue	648	1	Phosphoserine By similarity
	Modified residue	686	1	Phosphoserine By similarity
	Modified residue	699	1	Phosphoserine By similarity
Natural variations
	Alternative sequence	340 – 366	27	Missing in isoform 2.		VSP_003263

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 (A) [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 0F12F51902FC179A	FASTA	706	80,223
	10 20 30 40 50 60 MSPTDPLDDE DTFKILVATD IHLGFMEKDA VRGNDTFVTF DEILRLALEN EVDFILLGGD 70 80 90 100 110 120 LFHENKPSRK TLHSCLELLR KYCMGDRPVQ FEVISDQSVN FGFSKFPWVN YQDGNLNISI 130 140 150 160 170 180 PVFSIHGNHD DPTGADALCA LDVLSCAGFV NHFGRSMSVE KVDISPVLLQ KGSTKLALYG 190 200 210 220 230 240 LGSIPDERLY RMFVNKKVTM LRPKEDENSW FNLFVIHQNR SKHGNTNFIP EQFLDDFIDL 250 260 270 280 290 300 VIWGHEHECK IGPIKNEQQL FYVSQPGSSV VTSLSPGEAV KKHVGLLRIK GRKMNMQKLP 310 320 330 340 350 360 LRTVRRFFIE DVVLANHPNL FNPDNPKVTQ AIQSFCLEKI EEMLDSAERE RLGNPQQPGK 370 380 390 400 410 420 PLIRLRVDYS GGFEPFNVLR FSQKFVDRVA NPKDVIHFFR HREQKGKTGE EINFGMLITK 430 440 450 460 470 480 PASEGATLRV EDLVKQYFQT AEKNVQLSLL TERGMGEAVQ EFVDKEEKDA IEELVKYQLE 490 500 510 520 530 540 KTQRFLKERH IDALEDKIDE EVRRFRESRQ RNTNEEDDEV REAMSRARAL RSQSETSTSA 550 560 570 580 590 600 FSAEDLSFDT SEQTANDSDD SLSAVPSRGR GRGRGRRGAR GQSSAPRGGS QRGRDTGLEI 610 620 630 640 650 660 TTRGRSSKAT SSTSRNMSII DAFRSTRQQP SRNVAPKNYS ETIEVDDSDE DDIFPTNSRA 670 680 690 700 DQRWSGTTSS KRMSQSQTAK GVDFESDEDD DDDPFMSSSC PRRNRR
	Isoform 2 (B) [UniParc]. Checksum: 3ACDE474E0425430	FASTA	679	77,094
	10 20 30 40 50 60 MSPTDPLDDE DTFKILVATD IHLGFMEKDA VRGNDTFVTF DEILRLALEN EVDFILLGGD 70 80 90 100 110 120 LFHENKPSRK TLHSCLELLR KYCMGDRPVQ FEVISDQSVN FGFSKFPWVN YQDGNLNISI 130 140 150 160 170 180 PVFSIHGNHD DPTGADALCA LDVLSCAGFV NHFGRSMSVE KVDISPVLLQ KGSTKLALYG 190 200 210 220 230 240 LGSIPDERLY RMFVNKKVTM LRPKEDENSW FNLFVIHQNR SKHGNTNFIP EQFLDDFIDL 250 260 270 280 290 300 VIWGHEHECK IGPIKNEQQL FYVSQPGSSV VTSLSPGEAV KKHVGLLRIK GRKMNMQKLP 310 320 330 340 350 360 LRTVRRFFIE DVVLANHPNL FNPDNPKVTQ AIQSFCLEKV DYSGGFEPFN VLRFSQKFVD 370 380 390 400 410 420 RVANPKDVIH FFRHREQKGK TGEEINFGML ITKPASEGAT LRVEDLVKQY FQTAEKNVQL 430 440 450 460 470 480 SLLTERGMGE AVQEFVDKEE KDAIEELVKY QLEKTQRFLK ERHIDALEDK IDEEVRRFRE 490 500 510 520 530 540 SRQRNTNEED DEVREAMSRA RALRSQSETS TSAFSAEDLS FDTSEQTAND SDDSLSAVPS 550 560 570 580 590 600 RGRGRGRGRR GARGQSSAPR GGSQRGRDTG LEITTRGRSS KATSSTSRNM SIIDAFRSTR 610 620 630 640 650 660 QQPSRNVAPK NYSETIEVDD SDEDDIFPTN SRADQRWSGT TSSKRMSQSQ TAKGVDFESD 670 EDDDDDPFMS SSCPRRNRR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Oshiumi H., Shinohara A., Ogawa H. Submitted (MAY-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2). Strain: CD-1. Tissue: Testis.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: C57BL/6. Tissue: Retina.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U58987 mRNA. Translation: AAB04955.1. U60318 mRNA. Translation: AAB03664.1. BC065144 mRNA. Translation: AAH65144.1.
IPI	IPI00118853. IPI00283379.
RefSeq	NP_061206.1. NM_018736.2.
UniGene	Mm.149071.
3D structure databases
ProteinModelPortal	Q61216.
SMR	Q61216. Positions 8-400.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q61216. 3 interactions.
PTM databases
PhosphoSite	Q61216.
Proteomic databases
PaxDb	Q61216.
PRIDE	Q61216.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000034405; ENSMUSP00000034405; ENSMUSG00000031928. ENSMUST00000115632; ENSMUSP00000111295; ENSMUSG00000031928.
GeneID	17535.
KEGG	mmu:17535.
Organism-specific databases
CTD	4361.
MGI	MGI:1100512. Mre11a.
Phylogenomic databases
eggNOG	COG0420.
GeneTree	ENSGT00390000017288.
HOGENOM	HOG000216581.
HOVERGEN	HBG052508.
InParanoid	Q61216.
KO	K10865.
OMA	FVNKQVT.
OrthoDB	EOG46WZ82.
Gene expression databases
ArrayExpress	Q61216.
Bgee	Q61216.
Genevestigator	Q61216.
GermOnline	ENSMUSG00000031928. Mus musculus.
Family and domain databases
InterPro	IPR003701. DNA_repair_Mre11. IPR004843. Metallo_PEstase_dom. IPR007281. Mre11_DNA-bd. [Graphical view]
PANTHER	PTHR10139. PTHR10139. 1 hit.
Pfam	PF00149. Metallophos. 1 hit. PF04152. Mre11_DNA_bind. 1 hit. [Graphical view]
PIRSF	PIRSF000882. DSB_repair_MRE11. 1 hit.
TIGRFAMs	TIGR00583. mre11. 1 hit.
ProtoNet	Search...
Other
NextBio	292160.
PMAP-CutDB	Q61216.
SOURCE	Search...

Entry information

Entry name

MRE11_MOUSE

Accession

Primary (citable) accession number: Q61216
Secondary accession number(s): Q62430

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 26, 2001
Last sequence update:	November 1, 1996
Last modified:	April 3, 2013
This is version 101 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program