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Protein

Rho guanine nucleotide exchange factor 1

Gene

Arhgef1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Seems to play a role in the regulation of RhoA GTPase by guanine nucleotide-binding alpha-12 (GNA12) and alpha-13 (GNA13) subunits. Acts as GTPase-activating protein (GAP) for GNA12 and GNA13, and as guanine nucleotide exchange factor (GEF) for RhoA GTPase. Activated G alpha 13/GNA13 stimulates the RhoGEF activity through interaction with the RGS-like domain. This GEF activity is inhibited by binding to activated GNA12. Mediates angiotensin-2-induced RhoA activation. Isoform 3 and isoform 4 do not homooligomerize and show an enhanced RhoGEF activity.2 Publications

Caution

Ref. 2 sequence was originally submitted as from rat origin.Curated

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGTPase activation, Guanine-nucleotide releasing factor

Enzyme and pathway databases

ReactomeiR-MMU-193648 NRAGE signals death through JNK
R-MMU-194840 Rho GTPase cycle
R-MMU-416482 G alpha (12/13) signalling events

Names & Taxonomyi

Protein namesi
Recommended name:
Rho guanine nucleotide exchange factor 1
Alternative name(s):
Lbc's second cousin
Lymphoid blast crisis-like 2
Gene namesi
Name:Arhgef1
Synonyms:Lbcl2, Lsc
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi

Organism-specific databases

MGIiMGI:1353510 Arhgef1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Membrane

Pathology & Biotechi

Disruption phenotypei

Mice have reduced response to angiotensin-2 and lowered RhoA signaling pathway.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000809071 – 920Rho guanine nucleotide exchange factor 1Add BLAST920

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei373PhosphoserineBy similarity1
Modified residuei408PhosphoserineBy similarity1
Modified residuei694PhosphothreonineBy similarity1
Modified residuei737Phosphotyrosine; by JAK21 Publication1
Modified residuei905PhosphoserineCombined sources1
Isoform 5 (identifier: Q61210-5)
Modified residuei386PhosphoserineCombined sources1
Modified residuei390PhosphoserineCombined sources1
Modified residuei412PhosphoserineCombined sources1
Modified residuei432PhosphothreonineCombined sources1

Post-translational modificationi

Phosphorylated by PKCA (By similarity). Angiotensin-2 induced Tyr-737 phosphorylation is mediated by JAK2. Isoform 5 is phosphorylated at 'Ser-390'.By similarity1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ61210
PeptideAtlasiQ61210
PRIDEiQ61210

PTM databases

iPTMnetiQ61210
PhosphoSitePlusiQ61210
SwissPalmiQ61210

Expressioni

Tissue specificityi

Ubiquitously expressed.1 Publication

Gene expression databases

BgeeiENSMUSG00000040940
CleanExiMM_ARHGEF1
ExpressionAtlasiQ61210 baseline and differential
GenevisibleiQ61210 MM

Interactioni

Subunit structurei

Interacts with RHOA, GNA12 and GNA13 (By similarity). Homooligomerizes through the coiled coil region. Interacts with CTNNAL1 (By similarity). May interact with CCPG1.By similarity2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
VclQ647273EBI-641821,EBI-432047

GO - Molecular functioni

Protein-protein interaction databases

BioGridi201115, 5 interactors
IntActiQ61210, 12 interactors
MINTiQ61210

Structurei

3D structure databases

ProteinModelPortaliQ61210
SMRiQ61210
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini39 – 230RGSLAdd BLAST192
Domaini415 – 604DHPROSITE-ProRule annotationAdd BLAST190
Domaini646 – 759PHPROSITE-ProRule annotationAdd BLAST114

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili865 – 894Sequence analysisAdd BLAST30

Domaini

The RGSL domain, also known as rgRGS domain, is necessary but not sufficient for full GAP activity.By similarity
The DH domain is involved in interaction with CCPG1.

Keywords - Domaini

Coiled coil

Phylogenomic databases

GeneTreeiENSGT00760000119193
HOGENOMiHOG000034043
HOVERGENiHBG050565
InParanoidiQ61210
KOiK12330
OMAiFYHSFLD
OrthoDBiEOG091G0CZH
PhylomeDBiQ61210
TreeFamiTF106495

Family and domain databases

CDDicd08755 RGS_p115RhoGEF, 1 hit
cd00160 RhoGEF, 1 hit
Gene3Di1.20.900.10, 1 hit
2.30.29.30, 1 hit
InterProiView protein in InterPro
IPR035899 DBL_dom_sf
IPR000219 DH-domain
IPR037887 p115RhoGEF_RGS
IPR011993 PH-like_dom_sf
IPR001849 PH_domain
IPR015212 RGS-like_dom
IPR036305 RGS_sf
PfamiView protein in Pfam
PF09128 RGS-like, 1 hit
PF00621 RhoGEF, 1 hit
SMARTiView protein in SMART
SM00233 PH, 1 hit
SM00325 RhoGEF, 1 hit
SUPFAMiSSF48065 SSF48065, 1 hit
SSF48097 SSF48097, 1 hit
PROSITEiView protein in PROSITE
PS50010 DH_2, 1 hit
PS50003 PH_DOMAIN, 1 hit

Sequences (5)i

Sequence statusi: Complete.

This entry describes 5 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q61210-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGEVAGGAAP GPPRSGLVSI IIGAEDEDFE NELEANSEDQ NSQFQSLEQV
60 70 80 90 100
KRRPAHLMAL LQHVALQFEP GPLLCCLHAD MLSSLGPKEA KKAFLDFYHS
110 120 130 140 150
FLEKTAVLRV PVPPSVAFEL DRTRPDLISE DVQRRFIQEV VQSQQAAVSR
160 170 180 190 200
QLEDFRSKRL MGMTPWEQEL SLLEPWIGKD RGNYEARERH VAERLLSHLE
210 220 230 240 250
ETQHTISTDE EKSAAVVTAI SLYMRHLGVR TKSGDKKSGR NFFRKKVMGN
260 270 280 290 300
RRSDEPPKTK KGLSSILDPA RWNRGEPSAP DCRHLKVEAD AEKPGPADRK
310 320 330 340 350
GGLGMSSRDR TVGTPGQDNP GVSLHPLSTD SVDSREPGVD TPQEPGDTPP
360 370 380 390 400
QGPTSLEPLA PPESTEDNGE TESPEPGDDG EPGRSGLELE PEEPPGWREL
410 420 430 440 450
VPPDTLLSLP KSQVKRQEVI SELLVTEAAH VRMLRVLHDL FYQPMADGGF
460 470 480 490 500
FPLDELQNIF PSLDELIEVH SLFLDRLMKR RQESGYLIEE IGDVLLARFD
510 520 530 540 550
GAEGSWFQKI SSRFCSRQSF ALEQLKAKQR KEPRFCAFVQ EAESRPRCRR
560 570 580 590 600
LQLKDMIPTE MQRLTKYPLL LQSIGQNTEE STERGKVELA AECCREILHH
610 620 630 640 650
VNQAVRDMED LLRLKDYQRR LDLTHLRQSS DPMLSEFKNL DITKKKLVHE
660 670 680 690 700
GPLTWRVTKD KAIEVHVLLL DDLLLLLQRQ DERLLLKSHS RTLTPTPDGK
710 720 730 740 750
TMLRPVLRLT SAMTREVATD HKAFYVIFTW DQEAQIYELV AQTSSERKNW
760 770 780 790 800
CNLITETAGS LKVPAPASRL KPRPSPSSIR EPLLSSSENG TGGAEMAPAD
810 820 830 840 850
ARTERLLNDL LPFCRPGPEG QLAATALQKV LSLKQILLST EEDSGAGPPR
860 870 880 890 900
DGDGVPGGRA PGPVHTQEIE ENLLSLEVAI RQLEELEEEF CRLRPLLSQL
910 920
GGTLSPNLAA PERSAQTGLS
Length:920
Mass (Da):102,805
Last modified:August 29, 2003 - v2
Checksum:i913A819972CBACC8
GO
Isoform 2 (identifier: Q61210-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     291-291: Missing.

Show »
Length:919
Mass (Da):102,733
Checksum:i751EBF35E31F38BB
GO
Isoform 3 (identifier: Q61210-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     780-791: REPLLSSSENGT → SHPRGLEQREIG
     792-920: Missing.

Show »
Length:791
Mass (Da):89,294
Checksum:i07126CD40D75A2C1
GO
Isoform 4 (identifier: Q61210-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     830-856: VLSLKQILLSTEEDSGAGPPRDGDGVP → GVGRGILSPKTPPVPAWGDSVPQPGCT
     857-920: Missing.

Show »
Length:856
Mass (Da):95,823
Checksum:i99B42F760C3435C6
GO
Isoform 5 (identifier: Q61210-5) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     291-291: Missing.
     372-373: ES → ERRWKRLSGRLGRSESLRVSDRRRPSRGSLGAKGRGGGRSRSDVDMDPGSATAVLGPTRRAT

Show »
Length:979
Mass (Da):109,249
Checksum:iFEF62EF944FFABD6
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti574I → M in AAC36527 (PubMed:9798653).Curated1
Sequence conflicti663I → T in AAC36527 (PubMed:9798653).Curated1
Sequence conflicti689H → Y in AAO91659 (PubMed:12773540).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_008126291Missing in isoform 2 and isoform 5. 3 Publications1
Alternative sequenceiVSP_026131372 – 373ES → ERRWKRLSGRLGRSESLRVS DRRRPSRGSLGAKGRGGGRS RSDVDMDPGSATAVLGPTRR AT in isoform 5. 1 Publication2
Alternative sequenceiVSP_008127780 – 791REPLL…SENGT → SHPRGLEQREIG in isoform 3. 1 PublicationAdd BLAST12
Alternative sequenceiVSP_008128792 – 920Missing in isoform 3. 1 PublicationAdd BLAST129
Alternative sequenceiVSP_008129830 – 856VLSLK…GDGVP → GVGRGILSPKTPPVPAWGDS VPQPGCT in isoform 4. 1 PublicationAdd BLAST27
Alternative sequenceiVSP_008130857 – 920Missing in isoform 4. 1 PublicationAdd BLAST64

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U58203 mRNA Translation: AAC52693.1
AF314539 mRNA Translation: AAG33860.1
AK157056 mRNA Translation: BAE33947.1
AK172354 mRNA Translation: BAE42963.1
BC012488 mRNA Translation: AAH12488.1
U89421 mRNA Translation: AAC36527.1
AY246272 mRNA Translation: AAO91658.1
AY246273 mRNA Translation: AAO91659.1
CCDSiCCDS52141.1 [Q61210-1]
CCDS52142.1 [Q61210-5]
CCDS85239.1 [Q61210-2]
RefSeqiNP_001123622.1, NM_001130150.1 [Q61210-5]
NP_001123623.1, NM_001130151.1
NP_001123624.1, NM_001130152.1 [Q61210-1]
NP_001123625.1, NM_001130153.1 [Q61210-1]
NP_032514.1, NM_008488.2 [Q61210-2]
UniGeneiMm.3181

Genome annotation databases

EnsembliENSMUST00000047873; ENSMUSP00000046469; ENSMUSG00000040940 [Q61210-1]
ENSMUST00000098683; ENSMUSP00000096280; ENSMUSG00000040940 [Q61210-5]
ENSMUST00000117419; ENSMUSP00000113366; ENSMUSG00000040940 [Q61210-1]
ENSMUST00000206508; ENSMUSP00000146314; ENSMUSG00000040940 [Q61210-2]
GeneIDi16801
KEGGimmu:16801
UCSCiuc009fqv.2 mouse [Q61210-2]
uc009fqw.2 mouse [Q61210-5]
uc009fqy.2 mouse [Q61210-1]

Keywords - Coding sequence diversityi

Alternative splicing

Entry informationi

Entry nameiARHG1_MOUSE
AccessioniPrimary (citable) accession number: Q61210
Secondary accession number(s): O89074
, Q3T9Q7, Q80YE8, Q80YE9, Q91VL3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 29, 2003
Last sequence update: August 29, 2003
Last modified: March 28, 2018
This is version 144 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

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