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Protein

Prosaposin

Gene

Psap

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Prosaposin: Behaves as a myelinotrophic and neurotrophic factor, these effects are mediated by its G-protein-coupled receptors, GPR37 and GPR37L1, undergoing ligand-mediated internalization followed by ERK phosphorylation signaling.1 Publication
Saposin-A and saposin-C stimulate the hydrolysis of glucosylceramide by beta-glucosylceramidase (EC 3.2.1.45) and galactosylceramide by beta-galactosylceramidase (EC 3.2.1.46). Saposin-C apparently acts by combining with the enzyme and acidic lipid to form an activated complex, rather than by solubilizing the substrate.By similarity
Saposin-B stimulates the hydrolysis of galacto-cerebroside sulfate by arylsulfatase A (EC 3.1.6.8), GM1 gangliosides by beta-galactosidase (EC 3.2.1.23) and globotriaosylceramide by alpha-galactosidase A (EC 3.2.1.22). Saposin-B forms a solubilizing complex with the substrates of the sphingolipid hydrolases.By similarity
Saposin-D is a specific sphingomyelin phosphodiesterase activator (EC 3.1.4.12).By similarity
Saposins are specific low-molecular mass non-enzymic proteins, they participate in the lysosomal degradation of sphingolipids, which takes place by the sequential action of specific hydrolases.By similarity

GO - Molecular functioni

GO - Biological processi

  • adenylate cyclase-inhibiting G-protein coupled receptor signaling pathway Source: ParkinsonsUK-UCL
  • cellular response to organic substance Source: MGI
  • developmental growth Source: MGI
  • epithelial cell differentiation involved in prostate gland development Source: MGI
  • ganglioside GM1 transport to membrane Source: MGI
  • negative regulation of hydrogen peroxide-induced cell death Source: ParkinsonsUK-UCL
  • positive regulation of MAPK cascade Source: ParkinsonsUK-UCL
  • prostate gland growth Source: MGI
  • regulation of lipid metabolic process Source: MGI
  • regulation of MAPK cascade Source: MGI
  • sphingolipid metabolic process Source: InterPro

Enzyme and pathway databases

ReactomeiR-MMU-114608 Platelet degranulation
R-MMU-1660662 Glycosphingolipid metabolism
R-MMU-375276 Peptide ligand-binding receptors
R-MMU-418594 G alpha (i) signalling events
R-MMU-6798695 Neutrophil degranulation

Names & Taxonomyi

Protein namesi
Recommended name:
Prosaposin
Alternative name(s):
Sulfated glycoprotein 1
Short name:
SGP-1
Cleaved into the following 5 chains:
Gene namesi
Name:Psap
Synonyms:Sgp1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 10

Organism-specific databases

MGIiMGI:97783 Psap

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Lysosome, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 16By similarityAdd BLAST16
ChainiPRO_000003162617 – 557ProsaposinAdd BLAST541
PropeptideiPRO_000043495317 – 59By similarityAdd BLAST43
ChainiPRO_000043495460 – 142Saposin-ABy similarityAdd BLAST83
PropeptideiPRO_0000434955143 – 193By similarityAdd BLAST51
ChainiPRO_0000434956194 – 276Saposin-B-ValBy similarityAdd BLAST83
ChainiPRO_0000434957194 – 275Saposin-BBy similarityAdd BLAST82
PropeptideiPRO_0000434958277 – 312By similarityAdd BLAST36
ChainiPRO_0000434959313 – ?392Saposin-CBy similarityAdd BLAST80
PropeptideiPRO_0000434960?393 – 437By similarityAdd BLAST45
ChainiPRO_0000434961438 – 519Saposin-DBy similarityAdd BLAST82
PropeptideiPRO_0000434962520 – 557By similarityAdd BLAST38

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi63 ↔ 138PROSITE-ProRule annotation
Disulfide bondi66 ↔ 132PROSITE-ProRule annotation
Glycosylationi80N-linked (GlcNAc...) asparaginePROSITE-ProRule annotation1 Publication1
Disulfide bondi94 ↔ 106PROSITE-ProRule annotation
Disulfide bondi197 ↔ 273PROSITE-ProRule annotation
Disulfide bondi200 ↔ 267PROSITE-ProRule annotation
Glycosylationi214N-linked (GlcNAc...) asparaginePROSITE-ProRule annotation1
Disulfide bondi229 ↔ 240PROSITE-ProRule annotation
Disulfide bondi317 ↔ 390PROSITE-ProRule annotation
Disulfide bondi320 ↔ 384PROSITE-ProRule annotation
Glycosylationi334N-linked (GlcNAc...) asparaginePROSITE-ProRule annotation1 Publication1
Disulfide bondi348 ↔ 359PROSITE-ProRule annotation
Disulfide bondi442 ↔ 515PROSITE-ProRule annotation
Disulfide bondi445 ↔ 509PROSITE-ProRule annotation
Glycosylationi459N-linked (GlcNAc...) asparaginePROSITE-ProRule annotation1
Disulfide bondi473 ↔ 484PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

EPDiQ61207
MaxQBiQ61207
PaxDbiQ61207
PeptideAtlasiQ61207
PRIDEiQ61207

PTM databases

iPTMnetiQ61207
PhosphoSitePlusiQ61207

Expressioni

Gene expression databases

BgeeiENSMUSG00000004207
CleanExiMM_PSAP
ExpressionAtlasiQ61207 baseline and differential
GenevisibleiQ61207 MM

Interactioni

Subunit structurei

Saposin-B is a homodimer. Prosaposin exists as a roughly half-half mixture of monomers and disulfide-linked dimers. Monomeric prosaposin interacts (via C-terminus) with sortilin/SORT1, the interaction is required for targeting to lysosomes.By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi202410, 2 interactors
IntActiQ61207, 6 interactors
MINTiQ61207
STRINGi10090.ENSMUSP00000137476

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5NXBX-ray3.60C/D59-143[»]
ProteinModelPortaliQ61207
SMRiQ61207
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini18 – 58Saposin A-type 1PROSITE-ProRule annotationAdd BLAST41
Domaini59 – 142Saposin B-type 1PROSITE-ProRule annotationAdd BLAST84
Domaini193 – 277Saposin B-type 2PROSITE-ProRule annotationAdd BLAST85
Domaini313 – 394Saposin B-type 3PROSITE-ProRule annotationAdd BLAST82
Domaini438 – 519Saposin B-type 4PROSITE-ProRule annotationAdd BLAST82
Domaini521 – 557Saposin A-type 2PROSITE-ProRule annotationAdd BLAST37

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiKOG1340 Eukaryota
ENOG410XSI5 LUCA
GeneTreeiENSGT00530000063434
HOGENOMiHOG000049216
HOVERGENiHBG002617
InParanoidiQ61207
KOiK12382
OMAiCKNYINQ
PhylomeDBiQ61207

Family and domain databases

InterProiView protein in InterPro
IPR003119 SAP_A
IPR007856 SapB_1
IPR008138 SapB_2
IPR008373 Saposin
IPR011001 Saposin-like
IPR021165 Saposin_chordata
IPR008139 SaposinB_dom
PfamiView protein in Pfam
PF02199 SapA, 2 hits
PF05184 SapB_1, 4 hits
PF03489 SapB_2, 4 hits
PIRSFiPIRSF002431 Saposin, 1 hit
PRINTSiPR01797 SAPOSIN
SMARTiView protein in SMART
SM00162 SAPA, 2 hits
SM00741 SapB, 4 hits
SUPFAMiSSF47862 SSF47862, 3 hits
PROSITEiView protein in PROSITE
PS51110 SAP_A, 2 hits
PS50015 SAP_B, 4 hits

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q61207-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MYALALFASL LATALTSPVQ DPKTCSGGSA VLCRDVKTAV DCGAVKHCQQ
60 70 80 90 100
MVWSKPTAKS LPCDICKTVV TEAGNLLKDN ATQEEILHYL EKTCEWIHDS
110 120 130 140 150
SLSASCKEVV DSYLPVILDM IKGEMSNPGE VCSALNLCQS LQEYLAEQNQ
160 170 180 190 200
KQLESNKIPE VDMARVVAPF MSNIPLLLYP QDHPRSQPQP KANEDVCQDC
210 220 230 240 250
MKLVSDVQTA VKTNSSFIQG FVDHVKEDCD RLGPGVSDIC KNYVDQYSEV
260 270 280 290 300
CVQMLMHMQD QQPKEICVLA GFCNEVKRVP MKTLVPATET IKNILPALEM
310 320 330 340 350
MDPYEQNLVQ AHNVILCQTC QFVMNKFSEL IVNNATEELL VKGLSNACAL
360 370 380 390 400
LPDPARTKCQ EVVGTFGPSL LDIFIHEVNP SSLCGVIGLC AARPELVEAL
410 420 430 440 450
EQPAPAIVSA LLKEPTPPKQ PAQPKQSALP AHVPPQKNGG FCEVCKKLVL
460 470 480 490 500
YLEHNLEKNS TKEEILAALE KGCSFLPDPY QKQCDDFVAE YEPLLLEILV
510 520 530 540 550
EVMDPGFVCS KIGVCPSAYK LLLGTEKCVW GPSYWCQNME TAARCNAVDH

CKRHVWN
Length:557
Mass (Da):61,422
Last modified:December 15, 1998 - v2
Checksum:i134593E20499E35E
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti83Q → E in AAB31059 (PubMed:8334382).Curated1
Sequence conflicti158I → V in AAA92567 (PubMed:8565332).Curated1
Sequence conflicti160Missing in AAB31059 (PubMed:8334382).Curated1
Sequence conflicti171 – 172MS → SA in AAA92567 (PubMed:8565332).Curated2
Sequence conflicti244V → L in AAB31059 (PubMed:8334382).Curated1
Sequence conflicti254M → I in AAA92567 (PubMed:8565332).Curated1
Sequence conflicti255L → W in AAB31059 (PubMed:8334382).Curated1
Sequence conflicti260 – 262Missing (PubMed:8565332).Curated3
Sequence conflicti307N → D in AAB31059 (PubMed:8334382).Curated1
Sequence conflicti322F → L in AAB31059 (PubMed:8334382).Curated1
Sequence conflicti349 – 350AL → GV in AAB22175 (PubMed:1590788).Curated2
Sequence conflicti367G → D in AAA92567 (PubMed:8565332).Curated1
Sequence conflicti370L → Q in AAB31059 (PubMed:8334382).Curated1
Sequence conflicti373I → D in AAA92567 (PubMed:8565332).Curated1
Sequence conflicti391A → T (PubMed:8565332).Curated1
Sequence conflicti393R → L (PubMed:8565332).Curated1
Sequence conflicti406A → R (PubMed:8334382).Curated1
Sequence conflicti406A → R (PubMed:8565332).Curated1
Sequence conflicti430P → R in AAB31059 (PubMed:8334382).Curated1
Sequence conflicti445C → F in AAA92567 (PubMed:8565332).Curated1
Sequence conflicti448L → P in AAB02695 (Ref. 4) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S36200 mRNA Translation: AAB22175.1
S71616 mRNA Translation: AAB31059.1
U27340 mRNA Translation: AAA92567.1
U57999 Genomic DNA Translation: AAB02695.1
CCDSiCCDS35911.1
PIRiJH0604
RefSeqiNP_001139592.1, NM_001146120.1
NP_001139593.1, NM_001146121.1
NP_001139594.1, NM_001146122.1
NP_001139595.1, NM_001146123.1
NP_001139596.1, NM_001146124.1
NP_035309.3, NM_011179.3
UniGeneiMm.277498
Mm.29978

Genome annotation databases

EnsembliENSMUST00000179238; ENSMUSP00000137476; ENSMUSG00000004207
GeneIDi19156
KEGGimmu:19156
UCSCiuc033fpi.1 mouse

Similar proteinsi

Entry informationi

Entry nameiSAP_MOUSE
AccessioniPrimary (citable) accession number: Q61207
Secondary accession number(s): Q60861, Q64006, Q64219
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: December 15, 1998
Last modified: May 23, 2018
This is version 166 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references

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