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Protein

Prosaposin

Gene

Psap

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Prosaposin: Behaves as a myelinotrophic and neurotrophic factor, these effects are mediated by its G-protein-coupled receptors, GPR37 and GPR37L1, undergoing ligand-mediated internalization followed by ERK phosphorylation signaling.1 Publication

GO - Molecular functioni

  • G-protein coupled receptor binding Source: ParkinsonsUK-UCL

GO - Biological processi

  • adenylate cyclase-inhibiting G-protein coupled receptor signaling pathway Source: ParkinsonsUK-UCL
  • cellular response to organic substance Source: MGI
  • developmental growth Source: MGI
  • epithelial cell differentiation involved in prostate gland development Source: MGI
  • negative regulation of hydrogen peroxide-induced cell death Source: ParkinsonsUK-UCL
  • positive regulation of MAPK cascade Source: ParkinsonsUK-UCL
  • prostate gland growth Source: MGI
  • regulation of lipid metabolic process Source: MGI
  • regulation of MAPK cascade Source: MGI
  • sphingolipid metabolic process Source: InterPro
Complete GO annotation...

Enzyme and pathway databases

ReactomeiREACT_307071. Platelet degranulation.
REACT_319210. Peptide ligand-binding receptors.
REACT_339900. Glycosphingolipid metabolism.

Names & Taxonomyi

Protein namesi
Recommended name:
Prosaposin
Alternative name(s):
Sulfated glycoprotein 1
Short name:
SGP-1
Gene namesi
Name:Psap
Synonyms:Sgp1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 10

Organism-specific databases

MGIiMGI:97783. Psap.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1616By similarityAdd
BLAST
Chaini17 – 557541ProsaposinPRO_0000031626Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi63 ↔ 138PROSITE-ProRule annotation
Disulfide bondi66 ↔ 132PROSITE-ProRule annotation
Glycosylationi80 – 801N-linked (GlcNAc...)PROSITE-ProRule annotation1 Publication
Disulfide bondi94 ↔ 106PROSITE-ProRule annotation
Disulfide bondi197 ↔ 273PROSITE-ProRule annotation
Disulfide bondi200 ↔ 267PROSITE-ProRule annotation
Glycosylationi214 – 2141N-linked (GlcNAc...)PROSITE-ProRule annotation
Disulfide bondi229 ↔ 240PROSITE-ProRule annotation
Disulfide bondi317 ↔ 390PROSITE-ProRule annotation
Disulfide bondi320 ↔ 384PROSITE-ProRule annotation
Glycosylationi334 – 3341N-linked (GlcNAc...)PROSITE-ProRule annotation1 Publication
Disulfide bondi348 ↔ 359PROSITE-ProRule annotation
Disulfide bondi442 ↔ 515PROSITE-ProRule annotation
Disulfide bondi445 ↔ 509PROSITE-ProRule annotation
Glycosylationi459 – 4591N-linked (GlcNAc...)PROSITE-ProRule annotation
Disulfide bondi473 ↔ 484PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiQ61207.
PaxDbiQ61207.
PRIDEiQ61207.

PTM databases

PhosphoSiteiQ61207.

Expressioni

Gene expression databases

BgeeiQ61207.
CleanExiMM_PSAP.
ExpressionAtlasiQ61207. baseline and differential.
GenevisibleiQ61207. MM.

Interactioni

Protein-protein interaction databases

BioGridi202410. 2 interactions.
IntActiQ61207. 5 interactions.
MINTiMINT-1734080.
STRINGi10090.ENSMUSP00000137476.

Structurei

3D structure databases

ProteinModelPortaliQ61207.
SMRiQ61207. Positions 60-139, 195-274, 315-391, 439-517.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini18 – 5841Saposin A-type 1PROSITE-ProRule annotationAdd
BLAST
Domaini59 – 14284Saposin B-type 1PROSITE-ProRule annotationAdd
BLAST
Domaini193 – 27785Saposin B-type 2PROSITE-ProRule annotationAdd
BLAST
Domaini313 – 39482Saposin B-type 3PROSITE-ProRule annotationAdd
BLAST
Domaini438 – 51982Saposin B-type 4PROSITE-ProRule annotationAdd
BLAST
Domaini521 – 55737Saposin A-type 2PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 2 saposin A-type domains.PROSITE-ProRule annotation
Contains 4 saposin B-type domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiNOG269151.
GeneTreeiENSGT00530000063434.
HOGENOMiHOG000049216.
HOVERGENiHBG002617.
InParanoidiQ61207.
KOiK12382.
PhylomeDBiQ61207.

Family and domain databases

Gene3Di1.10.225.10. 4 hits.
InterProiIPR003119. SapA.
IPR007856. SapB_1.
IPR008138. SapB_2.
IPR008373. Saposin.
IPR011001. Saposin-like.
IPR021165. Saposin_chordata.
IPR008139. SaposinB.
[Graphical view]
PfamiPF02199. SapA. 2 hits.
PF05184. SapB_1. 4 hits.
PF03489. SapB_2. 4 hits.
[Graphical view]
PIRSFiPIRSF002431. Saposin. 1 hit.
PRINTSiPR01797. SAPOSIN.
SMARTiSM00162. SAPA. 2 hits.
SM00741. SapB. 4 hits.
[Graphical view]
SUPFAMiSSF47862. SSF47862. 3 hits.
PROSITEiPS51110. SAP_A. 2 hits.
PS50015. SAP_B. 4 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q61207-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MYALALFASL LATALTSPVQ DPKTCSGGSA VLCRDVKTAV DCGAVKHCQQ
60 70 80 90 100
MVWSKPTAKS LPCDICKTVV TEAGNLLKDN ATQEEILHYL EKTCEWIHDS
110 120 130 140 150
SLSASCKEVV DSYLPVILDM IKGEMSNPGE VCSALNLCQS LQEYLAEQNQ
160 170 180 190 200
KQLESNKIPE VDMARVVAPF MSNIPLLLYP QDHPRSQPQP KANEDVCQDC
210 220 230 240 250
MKLVSDVQTA VKTNSSFIQG FVDHVKEDCD RLGPGVSDIC KNYVDQYSEV
260 270 280 290 300
CVQMLMHMQD QQPKEICVLA GFCNEVKRVP MKTLVPATET IKNILPALEM
310 320 330 340 350
MDPYEQNLVQ AHNVILCQTC QFVMNKFSEL IVNNATEELL VKGLSNACAL
360 370 380 390 400
LPDPARTKCQ EVVGTFGPSL LDIFIHEVNP SSLCGVIGLC AARPELVEAL
410 420 430 440 450
EQPAPAIVSA LLKEPTPPKQ PAQPKQSALP AHVPPQKNGG FCEVCKKLVL
460 470 480 490 500
YLEHNLEKNS TKEEILAALE KGCSFLPDPY QKQCDDFVAE YEPLLLEILV
510 520 530 540 550
EVMDPGFVCS KIGVCPSAYK LLLGTEKCVW GPSYWCQNME TAARCNAVDH

CKRHVWN
Length:557
Mass (Da):61,422
Last modified:December 15, 1998 - v2
Checksum:i134593E20499E35E
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti83 – 831Q → E in AAB31059 (PubMed:8334382).Curated
Sequence conflicti158 – 1581I → V in AAA92567 (PubMed:8565332).Curated
Sequence conflicti160 – 1601Missing in AAB31059 (PubMed:8334382).Curated
Sequence conflicti171 – 1722MS → SA in AAA92567 (PubMed:8565332).Curated
Sequence conflicti244 – 2441V → L in AAB31059 (PubMed:8334382).Curated
Sequence conflicti254 – 2541M → I in AAA92567 (PubMed:8565332).Curated
Sequence conflicti255 – 2551L → W in AAB31059 (PubMed:8334382).Curated
Sequence conflicti260 – 2623Missing (PubMed:8565332).Curated
Sequence conflicti307 – 3071N → D in AAB31059 (PubMed:8334382).Curated
Sequence conflicti322 – 3221F → L in AAB31059 (PubMed:8334382).Curated
Sequence conflicti349 – 3502AL → GV in AAB22175 (PubMed:1590788).Curated
Sequence conflicti367 – 3671G → D in AAA92567 (PubMed:8565332).Curated
Sequence conflicti370 – 3701L → Q in AAB31059 (PubMed:8334382).Curated
Sequence conflicti373 – 3731I → D in AAA92567 (PubMed:8565332).Curated
Sequence conflicti391 – 3911A → T (PubMed:8565332).Curated
Sequence conflicti393 – 3931R → L (PubMed:8565332).Curated
Sequence conflicti406 – 4061A → R (PubMed:8334382).Curated
Sequence conflicti406 – 4061A → R (PubMed:8565332).Curated
Sequence conflicti430 – 4301P → R in AAB31059 (PubMed:8334382).Curated
Sequence conflicti445 – 4451C → F in AAA92567 (PubMed:8565332).Curated
Sequence conflicti448 – 4481L → P in AAB02695 (Ref. 4) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S36200 mRNA. Translation: AAB22175.1.
S71616 mRNA. Translation: AAB31059.1.
U27340 mRNA. Translation: AAA92567.1.
U57999 Genomic DNA. Translation: AAB02695.1.
CCDSiCCDS35911.1.
PIRiJH0604.
RefSeqiNP_001139592.1. NM_001146120.1.
NP_001139593.1. NM_001146121.1.
NP_001139594.1. NM_001146122.1.
NP_001139595.1. NM_001146123.1.
NP_001139596.1. NM_001146124.1.
NP_035309.3. NM_011179.3.
UniGeneiMm.277498.

Genome annotation databases

EnsembliENSMUST00000179238; ENSMUSP00000137476; ENSMUSG00000004207.
GeneIDi19156.
KEGGimmu:19156.
UCSCiuc007fen.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S36200 mRNA. Translation: AAB22175.1.
S71616 mRNA. Translation: AAB31059.1.
U27340 mRNA. Translation: AAA92567.1.
U57999 Genomic DNA. Translation: AAB02695.1.
CCDSiCCDS35911.1.
PIRiJH0604.
RefSeqiNP_001139592.1. NM_001146120.1.
NP_001139593.1. NM_001146121.1.
NP_001139594.1. NM_001146122.1.
NP_001139595.1. NM_001146123.1.
NP_001139596.1. NM_001146124.1.
NP_035309.3. NM_011179.3.
UniGeneiMm.277498.

3D structure databases

ProteinModelPortaliQ61207.
SMRiQ61207. Positions 60-139, 195-274, 315-391, 439-517.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi202410. 2 interactions.
IntActiQ61207. 5 interactions.
MINTiMINT-1734080.
STRINGi10090.ENSMUSP00000137476.

PTM databases

PhosphoSiteiQ61207.

Proteomic databases

MaxQBiQ61207.
PaxDbiQ61207.
PRIDEiQ61207.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000179238; ENSMUSP00000137476; ENSMUSG00000004207.
GeneIDi19156.
KEGGimmu:19156.
UCSCiuc007fen.2. mouse.

Organism-specific databases

CTDi5660.
MGIiMGI:97783. Psap.

Phylogenomic databases

eggNOGiNOG269151.
GeneTreeiENSGT00530000063434.
HOGENOMiHOG000049216.
HOVERGENiHBG002617.
InParanoidiQ61207.
KOiK12382.
PhylomeDBiQ61207.

Enzyme and pathway databases

ReactomeiREACT_307071. Platelet degranulation.
REACT_319210. Peptide ligand-binding receptors.
REACT_339900. Glycosphingolipid metabolism.

Miscellaneous databases

ChiTaRSiPsap. mouse.
NextBioi295806.
PROiQ61207.
SOURCEiSearch...

Gene expression databases

BgeeiQ61207.
CleanExiMM_PSAP.
ExpressionAtlasiQ61207. baseline and differential.
GenevisibleiQ61207. MM.

Family and domain databases

Gene3Di1.10.225.10. 4 hits.
InterProiIPR003119. SapA.
IPR007856. SapB_1.
IPR008138. SapB_2.
IPR008373. Saposin.
IPR011001. Saposin-like.
IPR021165. Saposin_chordata.
IPR008139. SaposinB.
[Graphical view]
PfamiPF02199. SapA. 2 hits.
PF05184. SapB_1. 4 hits.
PF03489. SapB_2. 4 hits.
[Graphical view]
PIRSFiPIRSF002431. Saposin. 1 hit.
PRINTSiPR01797. SAPOSIN.
SMARTiSM00162. SAPA. 2 hits.
SM00741. SapB. 4 hits.
[Graphical view]
SUPFAMiSSF47862. SSF47862. 3 hits.
PROSITEiPS51110. SAP_A. 2 hits.
PS50015. SAP_B. 4 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Liver.
  2. "Murine prosaposin: expression in the reproductive system of a gene implicated in human genetic diseases."
    Sprecher-Levy H., Orr-Urtreger A., Lonai P., Horowitz M.
    Cell. Mol. Biol. 39:287-299(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
  3. Erratum
    Sprecher-Levy H., Orr-Urtreger A., Lonai P., Horowitz M.
    Cell. Mol. Biol. 40:233-233(1994) [PubMed] [Europe PMC] [Abstract]
  4. "Expression of SGP-1 mRNA in preimplantation mouse embryos."
    Cao Q.P., Crain W.R.
    Dev. Genet. 17:263-271(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  5. Zhao Q.Q., Hay N.N., Morales C.R.
    Submitted (MAY-1996) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: BALB/c.
    Tissue: Liver.
  6. Lubec G., Kang S.U.
    Submitted (APR-2007) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 68-78 AND 463-482, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: C57BL/6.
    Tissue: Brain.
  7. "Mass-spectrometric identification and relative quantification of N-linked cell surface glycoproteins."
    Wollscheid B., Bausch-Fluck D., Henderson C., O'Brien R., Bibel M., Schiess R., Aebersold R., Watts J.D.
    Nat. Biotechnol. 27:378-386(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-80 AND ASN-334.
  8. "GPR37 and GPR37L1 are receptors for the neuroprotective and glioprotective factors prosaptide and prosaposin."
    Meyer R.C., Giddens M.M., Schaefer S.A., Hall R.A.
    Proc. Natl. Acad. Sci. U.S.A. 110:9529-9534(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.

Entry informationi

Entry nameiSAP_MOUSE
AccessioniPrimary (citable) accession number: Q61207
Secondary accession number(s): Q60861, Q64006, Q64219
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: December 15, 1998
Last modified: June 24, 2015
This is version 141 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.