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Q61205 (PA1B3_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 106. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Platelet-activating factor acetylhydrolase IB subunit gamma

EC=3.1.1.47
Alternative name(s):
PAF acetylhydrolase 29 kDa subunit
Short name=PAF-AH 29 kDa subunit
PAF-AH subunit gamma
Short name=PAFAH subunit gamma
Gene names
Name:Pafah1b3
Synonyms:Pafahg
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length232 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Inactivates paf by removing the acetyl group at the sn-2 position. This is a catalytic subunit. Plays an important role during the development of brain.

Catalytic activity

1-alkyl-2-acetyl-sn-glycero-3-phosphocholine + H2O = 1-alkyl-sn-glycero-3-phosphocholine + acetate.

Subunit structure

Cytosolic PAF-AH IB is formed of three subunits of 45 kDa (alpha), 30 kDa (beta) and 29 kDa (gamma). The catalytic activity of the enzyme resides in the beta and gamma subunits, whereas the alpha subunit has regulatory activity. Trimer formation is not essential for the catalytic activity.

Subcellular location

Cytoplasm.

Developmental stage

Expressed already by the time of neurulation. By E10.5, expression is abundant in the developing central and peripheral nervous systems. Major sites include the neuroepithelium of the fore-, mid-, and hindbrain, the spinal cord, the dorsal root, and cranial ganglia. In adult brain, expression is greatly diminished.

Sequence similarities

Belongs to the 'GDSL' lipolytic enzyme family. Platelet-activating factor acetylhydrolase IB beta/gamma subunits subfamily.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Pafah1b1P630052EBI-1007637,EBI-917499

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 232231Platelet-activating factor acetylhydrolase IB subunit gamma
PRO_0000058156

Sites

Active site481 By similarity
Active site1931 By similarity
Active site1961 By similarity

Amino acid modifications

Modified residue21N-acetylserine By similarity

Sequences

Sequence LengthMass (Da)Tools
Q61205 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 5D3C63E2A611670B

FASTA23225,853
        10         20         30         40         50         60 
MSGEGENPAS KPTPVQDVQG DGRWMSLHHR FVADSKDKEP EVVFIGDSLV QLMHQCEIWR 

        70         80         90        100        110        120 
ELFSPLHALN FGIGGDSTQH VLWRLENGEL EHIRPKIVVV WVGTNNHSHT AEQVTGGIKA 

       130        140        150        160        170        180 
IVQLVNKLQP QARVVVLGLL PRGQHPNPLR EKNRQVNELV RAALAGYPRA HFLDADPGFV 

       190        200        210        220        230 
HSDGTISHHD MYDYLHLSRL GYTPVCRALH SLLLRLLAQD QGQGIPLPET AS 

« Hide

References

« Hide 'large scale' references
[1]"Platelet-activating factor acetylhydrolase expression and activity suggest a link between neuronal migration and platelet-activating factor."
Albrecht U., Abu-Issa R., Raetz B., Hattori M., Aoki J., Arai H., Inoue K., Eichele G.
Dev. Biol. 180:579-593(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: BALB/c.
Tissue: Brain.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6.
Tissue: Brain.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U57746 mRNA. Translation: AAC52996.1.
BC067015 mRNA. Translation: AAH67015.1.
CCDSCCDS20979.1.
RefSeqNP_032802.1. NM_008776.2.
UniGeneMm.597.

3D structure databases

ProteinModelPortalQ61205.
SMRQ61205. Positions 6-208.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid202017. 2 interactions.
IntActQ61205. 2 interactions.
MINTMINT-4106434.

PTM databases

PhosphoSiteQ61205.

2D gel databases

REPRODUCTION-2DPAGEQ61205.

Proteomic databases

MaxQBQ61205.
PaxDbQ61205.
PRIDEQ61205.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000005583; ENSMUSP00000005583; ENSMUSG00000005447.
GeneID18476.
KEGGmmu:18476.
UCSCuc009fsf.1. mouse.

Organism-specific databases

CTD5050.
MGIMGI:108414. Pafah1b3.

Phylogenomic databases

eggNOGNOG69837.
GeneTreeENSGT00390000016520.
HOGENOMHOG000232143.
HOVERGENHBG053477.
InParanoidQ61205.
KOK16795.
OMAFAPLHCL.
PhylomeDBQ61205.
TreeFamTF323955.

Gene expression databases

ArrayExpressQ61205.
BgeeQ61205.
GenevestigatorQ61205.

Family and domain databases

Gene3D3.40.50.1110. 1 hit.
InterProIPR013831. SGNH_hydro-type_esterase_dom.
[Graphical view]
ProtoNetSearch...

Other

NextBio294174.
PROQ61205.
SOURCESearch...

Entry information

Entry namePA1B3_MOUSE
AccessionPrimary (citable) accession number: Q61205
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: November 1, 1996
Last modified: July 9, 2014
This is version 106 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot