Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Ral guanine nucleotide dissociation stimulator-like 2

Gene

Rgl2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Probable guanine nucleotide exchange factor. Putative effector of Ras and/or Rap. Associates with the GTP-bound form of Rap 1A and H-Ras in vitro.

GO - Molecular functioni

  • Rho guanyl-nucleotide exchange factor activity Source: MGI

GO - Biological processi

  • negative regulation of cardiac muscle cell apoptotic process Source: MGI
  • positive regulation of GTPase activity Source: GOC
  • positive regulation of phosphatidylinositol 3-kinase signaling Source: MGI
  • regulation of Ral protein signal transduction Source: MGI
  • small GTPase mediated signal transduction Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Guanine-nucleotide releasing factor

Enzyme and pathway databases

BRENDAi6.2.1.3. 3474.

Names & Taxonomyi

Protein namesi
Recommended name:
Ral guanine nucleotide dissociation stimulator-like 2
Short name:
RalGDS-like 2
Alternative name(s):
RalGDS-like factor
Ras-associated protein RAB2L
Gene namesi
Name:Rgl2
Synonyms:Rab2l, Rlf
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 17

Organism-specific databases

MGIiMGI:107483. Rgl2.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 778778Ral guanine nucleotide dissociation stimulator-like 2PRO_0000068889Add
BLAST

Proteomic databases

EPDiQ61193.
MaxQBiQ61193.
PaxDbiQ61193.
PRIDEiQ61193.

PTM databases

iPTMnetiQ61193.
PhosphoSiteiQ61193.

Expressioni

Gene expression databases

BgeeiQ61193.
CleanExiMM_RLF.
ExpressionAtlasiQ61193. baseline and differential.
GenevisibleiQ61193. MM.

Interactioni

Subunit structurei

Interacts with SAMD9.By similarity

Protein-protein interaction databases

IntActiQ61193. 3 interactions.
MINTiMINT-1582342.
STRINGi10090.ENSMUSP00000041082.

Structurei

Secondary structure

1
778
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi52 – 6211Combined sources
Beta strandi65 – 7410Combined sources
Beta strandi87 – 959Combined sources
Helixi97 – 1048Combined sources
Helixi109 – 1124Combined sources
Helixi116 – 1238Combined sources
Helixi124 – 1263Combined sources
Helixi130 – 14213Combined sources
Turni143 – 1464Combined sources
Helixi149 – 16921Combined sources
Helixi171 – 1733Combined sources
Helixi176 – 1783Combined sources
Helixi179 – 19214Combined sources
Helixi205 – 2139Combined sources
Helixi236 – 2416Combined sources
Helixi244 – 26017Combined sources
Helixi264 – 2674Combined sources
Turni269 – 2713Combined sources
Turni273 – 2764Combined sources
Helixi285 – 30521Combined sources
Beta strandi308 – 3103Combined sources
Beta strandi312 – 3143Combined sources
Beta strandi318 – 3203Combined sources
Helixi325 – 34420Combined sources
Helixi348 – 35912Combined sources
Helixi361 – 3644Combined sources
Helixi367 – 3715Combined sources
Helixi375 – 38511Combined sources
Turni398 – 4003Combined sources
Helixi433 – 44513Combined sources
Helixi457 – 47721Combined sources
Helixi486 – 4949Combined sources
Helixi500 – 51011Combined sources
Beta strandi650 – 6578Combined sources
Beta strandi660 – 6623Combined sources
Beta strandi668 – 6725Combined sources
Helixi680 – 6878Combined sources
Turni688 – 6914Combined sources
Beta strandi698 – 7025Combined sources
Turni703 – 7064Combined sources
Helixi719 – 7224Combined sources
Beta strandi727 – 7337Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1RLFNMR-A646-735[»]
4JGWX-ray2.30A/B50-514[»]
5CM8X-ray2.60A50-514[»]
5CM9X-ray2.60A/B50-514[»]
ProteinModelPortaliQ61193.
SMRiQ61193. Positions 53-514, 646-735.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ61193.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini88 – 212125N-terminal Ras-GEFPROSITE-ProRule annotationAdd
BLAST
Domaini243 – 513271Ras-GEFPROSITE-ProRule annotationAdd
BLAST
Domaini649 – 73688Ras-associatingPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 N-terminal Ras-GEF domain.PROSITE-ProRule annotation
Contains 1 Ras-associating domain.PROSITE-ProRule annotation
Contains 1 Ras-GEF domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG410KDSG. Eukaryota.
ENOG4110VI4. LUCA.
GeneTreeiENSGT00810000125349.
HOGENOMiHOG000231592.
HOVERGENiHBG005864.
InParanoidiQ61193.
KOiK17636.
OMAiNLVPSQC.
OrthoDBiEOG76DTRS.
PhylomeDBiQ61193.
TreeFamiTF315204.

Family and domain databases

Gene3Di1.10.840.10. 1 hit.
InterProiIPR000159. RA_dom.
IPR000651. Ras-like_Gua-exchang_fac_N.
IPR019804. Ras_G-nucl-exch_fac_CS.
IPR023578. Ras_GEF_dom.
IPR001895. RASGEF_cat_dom.
IPR029071. Ubiquitin-rel_dom.
[Graphical view]
PfamiPF00788. RA. 1 hit.
PF00617. RasGEF. 1 hit.
PF00618. RasGEF_N. 1 hit.
[Graphical view]
SMARTiSM00314. RA. 1 hit.
SM00147. RasGEF. 1 hit.
SM00229. RasGEFN. 1 hit.
[Graphical view]
SUPFAMiSSF48366. SSF48366. 1 hit.
SSF54236. SSF54236. 1 hit.
PROSITEiPS50200. RA. 1 hit.
PS00720. RASGEF. 1 hit.
PS50009. RASGEF_CAT. 1 hit.
PS50212. RASGEF_NTER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q61193-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLPRPLRLLL DTTPPGGVVL SSFRSRDPEE GGDPGGRAVG GGQEEEDEEE
60 70 80 90 100
EEASVSVWDE EEDGATFTVT SRQYRPLDPL APLPPPRSSR RLRAGTLEAL
110 120 130 140 150
VRHLLDARTA GADMMFTPAL LATHRAFTST PALFGLVADR LEALESHPPG
160 170 180 190 200
ELERTTGVAI SVLSTWLASH PEDFGSEVKG QLDRLESFLL RTGYAAREGV
210 220 230 240 250
VGGSADLIRN LRARVDPRAP DLPKPLALPG DSPADPTDVL VFLADHLAEQ
260 270 280 290 300
LTLLDAELFL NLIPSQCLGG LWGHRDRPGH SHLCPSVRAT VTQFNKVAGA
310 320 330 340 350
VVSSVLGATS IGEGPREVTV RPLRPPQRAR LLEKWIRVAE ECRLLRNFSS
360 370 380 390 400
VYAVVSALQS SPIHRLRAAW GETTRDSLRV FSSLCQIFSE EDNYSQSREL
410 420 430 440 450
LMQEVKPQPP VEPHSKKAPR SGFRGGGVVP YLGTFLKDLV MLDAASKDEL
460 470 480 490 500
ENGYINFDKR RKEFAILSEL LRLQKECRGY DLRPNSDIQQ WLQGLQPLTE
510 520 530 540 550
AQSHRVSCEV EPPGTSDSPA ARTPRPTLVI TQWTEVLGSV GGPTPLVSWD
560 570 580 590 600
RPSVGGDEVP GTPAPLLTRL AQHMKWPSVS SLDSALESSP SLHSPADPGH
610 620 630 640 650
LSPPASSPRP SRGHRRSASC GSPLSGNTGE GTSRSAGCGG GVSGPGSSDC
660 670 680 690 700
RIIRVQMELG EDGSVYKSIL VTSQDKAPSV ISRVLKKNNR DSAVASEFEL
710 720 730 740 750
VQLLPGDREL TIPHSANVFY AMDGASHDFL LRQRRRPSAA TPGSHSGPSA
760 770
SGTPPSEGGG GSFPRIKATG RKIARALF
Length:778
Mass (Da):83,826
Last modified:June 20, 2001 - v2
Checksum:iB23CB882A5416564
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti147 – 1471H → Y in AAC52724 (PubMed:8710374).Curated
Sequence conflicti402 – 4021M → T in AAC52724 (PubMed:8710374).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U54639 mRNA. Translation: AAC52724.1.
AF110520 Genomic DNA. Translation: AAC97974.1.
AF100956 Genomic DNA. Translation: AAC69894.1.
BC068121 mRNA. Translation: AAH68121.1.
CCDSiCCDS28635.1.
PIRiPC4379.
RefSeqiNP_033085.2. NM_009059.2.
XP_011237362.1. XM_011239060.1.
XP_011249601.1. XM_011251299.1.
UniGeneiMm.43777.

Genome annotation databases

EnsembliENSMUST00000047503; ENSMUSP00000041082; ENSMUSG00000041354.
GeneIDi19732.
KEGGimmu:19732.
UCSCiuc008cag.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U54639 mRNA. Translation: AAC52724.1.
AF110520 Genomic DNA. Translation: AAC97974.1.
AF100956 Genomic DNA. Translation: AAC69894.1.
BC068121 mRNA. Translation: AAH68121.1.
CCDSiCCDS28635.1.
PIRiPC4379.
RefSeqiNP_033085.2. NM_009059.2.
XP_011237362.1. XM_011239060.1.
XP_011249601.1. XM_011251299.1.
UniGeneiMm.43777.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1RLFNMR-A646-735[»]
4JGWX-ray2.30A/B50-514[»]
5CM8X-ray2.60A50-514[»]
5CM9X-ray2.60A/B50-514[»]
ProteinModelPortaliQ61193.
SMRiQ61193. Positions 53-514, 646-735.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ61193. 3 interactions.
MINTiMINT-1582342.
STRINGi10090.ENSMUSP00000041082.

PTM databases

iPTMnetiQ61193.
PhosphoSiteiQ61193.

Proteomic databases

EPDiQ61193.
MaxQBiQ61193.
PaxDbiQ61193.
PRIDEiQ61193.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000047503; ENSMUSP00000041082; ENSMUSG00000041354.
GeneIDi19732.
KEGGimmu:19732.
UCSCiuc008cag.1. mouse.

Organism-specific databases

CTDi5863.
MGIiMGI:107483. Rgl2.

Phylogenomic databases

eggNOGiENOG410KDSG. Eukaryota.
ENOG4110VI4. LUCA.
GeneTreeiENSGT00810000125349.
HOGENOMiHOG000231592.
HOVERGENiHBG005864.
InParanoidiQ61193.
KOiK17636.
OMAiNLVPSQC.
OrthoDBiEOG76DTRS.
PhylomeDBiQ61193.
TreeFamiTF315204.

Enzyme and pathway databases

BRENDAi6.2.1.3. 3474.

Miscellaneous databases

EvolutionaryTraceiQ61193.
PROiQ61193.
SOURCEiSearch...

Gene expression databases

BgeeiQ61193.
CleanExiMM_RLF.
ExpressionAtlasiQ61193. baseline and differential.
GenevisibleiQ61193. MM.

Family and domain databases

Gene3Di1.10.840.10. 1 hit.
InterProiIPR000159. RA_dom.
IPR000651. Ras-like_Gua-exchang_fac_N.
IPR019804. Ras_G-nucl-exch_fac_CS.
IPR023578. Ras_GEF_dom.
IPR001895. RASGEF_cat_dom.
IPR029071. Ubiquitin-rel_dom.
[Graphical view]
PfamiPF00788. RA. 1 hit.
PF00617. RasGEF. 1 hit.
PF00618. RasGEF_N. 1 hit.
[Graphical view]
SMARTiSM00314. RA. 1 hit.
SM00147. RasGEF. 1 hit.
SM00229. RasGEFN. 1 hit.
[Graphical view]
SUPFAMiSSF48366. SSF48366. 1 hit.
SSF54236. SSF54236. 1 hit.
PROSITEiPS50200. RA. 1 hit.
PS00720. RASGEF. 1 hit.
PS50009. RASGEF_CAT. 1 hit.
PS50212. RASGEF_NTER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "RalGDS-like factor (Rlf) is a novel Ras and Rap 1A-associating protein."
    Wolthuis R.M.F., Bauer B., Van'T Veer L.J., de Vries-Smits A.M.M., Cool R.H., Spaargaren M., Wittinghofer A., Burgering B.M.T., Bos J.L.
    Oncogene 13:353-362(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Sequence of the mouse major histocompatibility complex class II region."
    Rowen L., Qin S., Madan A., Loretz C., Hall J., James R., Dors M., Shaffer T., Abbasi N., Ratcliffe A., Dickhoff R., Lasky S., Hood L.
    Submitted (DEC-1998) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 129/SvJ.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
  4. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brown adipose tissue, Kidney, Lung, Spleen and Testis.
  5. "Structure determination of the Ras-binding domain of the Ral-specific guanine nucleotide exchange factor Rlf."
    Esser D., Bauer B., Wolthuis R.M., Wittinghofer A., Cool R.H., Bayer P.
    Biochemistry 37:13453-13462(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR OF 646-735.

Entry informationi

Entry nameiRGL2_MOUSE
AccessioniPrimary (citable) accession number: Q61193
Secondary accession number(s): Q9QUJ2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: June 20, 2001
Last modified: June 8, 2016
This is version 144 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.