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Protein

Tumor susceptibility gene 101 protein

Gene

Tsg101

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the ESCRT-I complex, a regulator of vesicular trafficking process. Binds to ubiquitinated cargo proteins and is required for the sorting of endocytic ubiquitinated cargos into multivesicular bodies (MVBs). Mediates the association between the ESCRT-0 and ESCRT-I complex. Required for completion of cytokinesis; the function requires CEP55. May be involved in cell growth and differentiation. Acts as a negative growth regulator. Required for the exosomal release of SDCBP, CD63 and syndecan (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

  • cell cycle arrest Source: MGI
  • cell differentiation Source: MGI
  • cell division Source: UniProtKB-KW
  • endosome to lysosome transport Source: Ensembl
  • exosomal secretion Source: ParkinsonsUK-UCL
  • keratinocyte differentiation Source: MGI
  • negative regulation of cell proliferation Source: MGI
  • negative regulation of transcription, DNA-templated Source: MGI
  • positive regulation of exosomal secretion Source: MGI
  • positive regulation of ubiquitin-dependent endocytosis Source: UniProtKB
  • positive regulation of viral budding via host ESCRT complex Source: MGI
  • positive regulation of viral process Source: MGI
  • positive regulation of viral release from host cell Source: MGI
  • protein monoubiquitination Source: Ensembl
  • protein transport Source: UniProtKB-KW
  • regulation of cell growth Source: MGI
  • regulation of extracellular exosome assembly Source: MGI
  • regulation of MAP kinase activity Source: MGI
  • regulation of viral budding via host ESCRT complex Source: MGI
  • ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway Source: UniProtKB
  • viral budding Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division, Growth regulation, Host-virus interaction, Protein transport, Transport

Enzyme and pathway databases

ReactomeiR-MMU-917729. Endosomal Sorting Complex Required For Transport (ESCRT).

Names & Taxonomyi

Protein namesi
Recommended name:
Tumor susceptibility gene 101 protein
Alternative name(s):
ESCRT-I complex subunit TSG101
Gene namesi
Name:Tsg101
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:106581. Tsg101.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB
  • early endosome Source: MGI
  • endosome Source: MGI
  • ESCRT I complex Source: UniProtKB
  • extracellular exosome Source: MGI
  • late endosome Source: MGI
  • late endosome membrane Source: UniProtKB-SubCell
  • nucleolus Source: MGI
  • nucleus Source: MGI
  • plasma membrane Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Endosome, Membrane, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 391390Tumor susceptibility gene 101 proteinPRO_0000082607Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineBy similarity
Modified residuei221 – 2211PhosphothreonineBy similarity

Post-translational modificationi

Monoubiquitinated at multiple sites by LRSAM1 and by MGRN1. Ubiquitination inactivates it, possibly by regulating its shuttling between an active membrane-bound protein and an inactive soluble form. Ubiquitination by MGRN1 requires the presence of UBE2D1 (By similarity).By similarity

Keywords - PTMi

Acetylation, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ61187.
MaxQBiQ61187.
PaxDbiQ61187.
PRIDEiQ61187.

PTM databases

iPTMnetiQ61187.
PhosphoSiteiQ61187.

Expressioni

Tissue specificityi

Ubiquitous. Higher expression in brain and mammary gland. Lower expression in liver and tumoral tissues.

Developmental stagei

Expressed at all stages of mammary gland development, but at lower rate at early and mid pregnancy. Expressed in 1-cell and 2-cell stage embryos.

Gene expression databases

BgeeiQ61187.
CleanExiMM_TSG101.
ExpressionAtlasiQ61187. baseline and differential.
GenevisibleiQ61187. MM.

Interactioni

Subunit structurei

Component of the ESCRT-I complex (endosomal sorting complex required for transport I) which consists of TSG101, VPS28, a VPS37 protein (VPS37A to -D) and MVB12A or MVB12B in a 1:1:1:1 stoechiometry. Interacts with VPS37A, VPS37B and VPS37C. Component of an ESCRT-I complex (endosomal sorting complex required for transport I) which consists of TSG101, VPS28, VPS37A and UBAP1 in a 1:1:1:1 stoechiometry. Interacts with ubiquitin, stathmin, GMCL, DMAP1 and AATF (By similarity). Interacts with HGS; the interaction mediates the association with the ESCRT-0 complex. Interacts with GGA1 and GGA3. Interacts (via UEV domain) with PDCD6IP/AIP1. Interacts with VPS28, SNF8 and VPS36. Self-associates. Interacts with MVB12A; the association appears to be mediated by the TSG101-VPS37 binary subcomplex. Interacts with VPS37D. Interacts with LRSAM1. Interacts with CEP55; the interaction is required for cytokinesis (By similarity). Interacts with PDCD6 (By similarity). Interacts with murine leukemia virus Gag polyprotein (via PSAP motif). Interacts with murine leukemia virus Gag polyprotein (via PSAP motif). Interacts with MGRN1.By similarity5 Publications

GO - Molecular functioni

Protein-protein interaction databases

BioGridi204341. 29 interactions.
IntActiQ61187. 21 interactions.
MINTiMINT-235334.
STRINGi10090.ENSMUSP00000014546.

Structurei

3D structure databases

ProteinModelPortaliQ61187.
SMRiQ61187. Positions 3-145, 229-380.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini2 – 145144UEVPROSITE-ProRule annotationAdd
BLAST
Domaini323 – 39169SBPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni159 – 1635Interaction with CEP55By similarity

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili237 – 31781Sequence analysisAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi321 – 3244PTAP/PSAP motif

Domaini

The UEV domain is required for the interaction of the complex with ubiquitin.
The coiled coil domain may interact with stathmin.

Sequence similaritiesi

Contains 1 SB (steadiness box) domain.PROSITE-ProRule annotation
Contains 1 UEV (ubiquitin E2 variant) domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG2391. Eukaryota.
ENOG410XT2Q. LUCA.
GeneTreeiENSGT00530000064004.
HOGENOMiHOG000247008.
HOVERGENiHBG057450.
InParanoidiQ61187.
KOiK12183.
OMAiPVMDSYV.
OrthoDBiEOG7JT6WH.
PhylomeDBiQ61187.
TreeFamiTF312917.

Family and domain databases

Gene3Di3.10.110.10. 1 hit.
InterProiIPR017916. SB_dom.
IPR016135. UBQ-conjugating_enzyme/RWD.
IPR008883. UEV_N.
[Graphical view]
PfamiPF05743. UEV. 1 hit.
PF09454. Vps23_core. 1 hit.
[Graphical view]
SUPFAMiSSF54495. SSF54495. 1 hit.
PROSITEiPS51312. SB. 1 hit.
PS51322. UEV. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q61187-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAVSESQLKK MMSKYKYRDL TVRQTVNVIA MYKDLKPVLD SYVFNDGSSR
60 70 80 90 100
ELVNLTGTIP VRYRGNIYNI PICLWLLDTY PYNPPICFVK PTSSMTIKTG
110 120 130 140 150
KHVDANGKIY LPYLHDWKHP RSELLELIQI MIVIFGEEPP VFSRPTVSAS
160 170 180 190 200
YPPYTATGPP NTSYMPGMPS GISAYPSGYP PNPSGYPGCP YPPAGPYPAT
210 220 230 240 250
TSSQYPSQPP VTTVGPSRDG TISEDTIRAS LISAVSDKLR WRMKEEMDGA
260 270 280 290 300
QAELNALKRT EEDLKKGHQK LEEMVTRLDQ EVAEVDKNIE LLKKKDEELS
310 320 330 340 350
SALEKMENQS ENNDIDEVII PTAPLYKQIL NLYAEENAIE DTIFYLGEAL
360 370 380 390
RRGVIDLDVF LKHVRLLSRK QFQLRALMQK ARKTAGLSDL Y
Length:391
Mass (Da):44,124
Last modified:August 1, 1998 - v2
Checksum:i79558EC535093492
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U52945 mRNA. Translation: AAC53586.1.
AF060868 Genomic DNA. Translation: AAC83576.1.
BC005424 mRNA. Translation: AAH05424.1.
BC085308 mRNA. Translation: AAH85308.1.
CCDSiCCDS21291.1.
RefSeqiNP_068684.1. NM_021884.3.
UniGeneiMm.241334.

Genome annotation databases

EnsembliENSMUST00000014546; ENSMUSP00000014546; ENSMUSG00000014402.
GeneIDi22088.
KEGGimmu:22088.
UCSCiuc009gzq.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U52945 mRNA. Translation: AAC53586.1.
AF060868 Genomic DNA. Translation: AAC83576.1.
BC005424 mRNA. Translation: AAH05424.1.
BC085308 mRNA. Translation: AAH85308.1.
CCDSiCCDS21291.1.
RefSeqiNP_068684.1. NM_021884.3.
UniGeneiMm.241334.

3D structure databases

ProteinModelPortaliQ61187.
SMRiQ61187. Positions 3-145, 229-380.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi204341. 29 interactions.
IntActiQ61187. 21 interactions.
MINTiMINT-235334.
STRINGi10090.ENSMUSP00000014546.

PTM databases

iPTMnetiQ61187.
PhosphoSiteiQ61187.

Proteomic databases

EPDiQ61187.
MaxQBiQ61187.
PaxDbiQ61187.
PRIDEiQ61187.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000014546; ENSMUSP00000014546; ENSMUSG00000014402.
GeneIDi22088.
KEGGimmu:22088.
UCSCiuc009gzq.1. mouse.

Organism-specific databases

CTDi7251.
MGIiMGI:106581. Tsg101.

Phylogenomic databases

eggNOGiKOG2391. Eukaryota.
ENOG410XT2Q. LUCA.
GeneTreeiENSGT00530000064004.
HOGENOMiHOG000247008.
HOVERGENiHBG057450.
InParanoidiQ61187.
KOiK12183.
OMAiPVMDSYV.
OrthoDBiEOG7JT6WH.
PhylomeDBiQ61187.
TreeFamiTF312917.

Enzyme and pathway databases

ReactomeiR-MMU-917729. Endosomal Sorting Complex Required For Transport (ESCRT).

Miscellaneous databases

ChiTaRSiTsg101. mouse.
PROiQ61187.
SOURCEiSearch...

Gene expression databases

BgeeiQ61187.
CleanExiMM_TSG101.
ExpressionAtlasiQ61187. baseline and differential.
GenevisibleiQ61187. MM.

Family and domain databases

Gene3Di3.10.110.10. 1 hit.
InterProiIPR017916. SB_dom.
IPR016135. UBQ-conjugating_enzyme/RWD.
IPR008883. UEV_N.
[Graphical view]
PfamiPF05743. UEV. 1 hit.
PF09454. Vps23_core. 1 hit.
[Graphical view]
SUPFAMiSSF54495. SSF54495. 1 hit.
PROSITEiPS51312. SB. 1 hit.
PS51322. UEV. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Tsg101: a novel tumor susceptibility gene isolated by controlled homozygous functional knockout of allelic loci in mammalian cells."
    Li L., Cohen S.N.
    Cell 85:319-329(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Fibroblast.
  2. "Genomic architecture and transcriptional activation of the mouse and human tumor susceptibility gene TSG101: common types of shorter transcripts are true alternative splice variants."
    Wagner K.-U., Dierisseau P., Rucker E.B. III, Robinson G.W., Hennighausen L.
    Oncogene 17:2761-2770(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 129/SvJ.
    Tissue: Mammary gland.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J and FVB/N.
    Tissue: Colon and Mammary gland.
  4. "DNMT1 binds HDAC2 and a new co-repressor, DMAP1, to form a complex at replication foci."
    Rountree M.R., Bachman K.E., Baylin S.B.
    Nat. Genet. 25:269-277(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH DMAP1.
  5. "Enhanced degradation of MDM2 by a nuclear envelope component, mouse germ cell-less."
    Masuhara M., Nagao K., Nishikawa M., Kimura T., Nakano T.
    Biochem. Biophys. Res. Commun. 308:927-932(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH GMCL.
  6. "TSG101 interaction with HRS mediates endosomal trafficking and receptor down-regulation."
    Lu Q., Hope L.W., Brasch M., Reinhard C., Cohen S.N.
    Proc. Natl. Acad. Sci. U.S.A. 100:7626-7631(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH HGS.
  7. "Tsg101 and Alix interact with murine leukemia virus Gag and cooperate with Nedd4 ubiquitin ligases during budding."
    Segura-Morales C., Pescia C., Chatellard-Causse C., Sadoul R., Bertrand E., Basyuk E.
    J. Biol. Chem. 280:27004-27012(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH MURINE LEUKEMIA VIRUS GAG POLYPROTEIN.
  8. "Abnormal regulation of TSG101 in mice with spongiform neurodegeneration."
    Jiao J., Sun K., Walker W.P., Bagher P., Cota C.D., Gunn T.M.
    Biochim. Biophys. Acta 1792:1027-1035(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH MGRN1, UBIQUITINATION BY MGRN1.
  9. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Heart, Kidney, Lung, Pancreas, Spleen and Testis.

Entry informationi

Entry nameiTS101_MOUSE
AccessioniPrimary (citable) accession number: Q61187
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: August 1, 1998
Last modified: July 6, 2016
This is version 159 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.