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Protein

Tumor susceptibility gene 101 protein

Gene

Tsg101

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the ESCRT-I complex, a regulator of vesicular trafficking process. Binds to ubiquitinated cargo proteins and is required for the sorting of endocytic ubiquitinated cargos into multivesicular bodies (MVBs). Mediates the association between the ESCRT-0 and ESCRT-I complex. Required for completion of cytokinesis; the function requires CEP55. May be involved in cell growth and differentiation. Acts as a negative growth regulator. Required for the exosomal release of SDCBP, CD63 and syndecan (By similarity). It may also play a role in the extracellular release of microvesicles that differ from the exosomes (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processCell cycle, Cell division, Growth regulation, Host-virus interaction, Protein transport, Transport

Enzyme and pathway databases

ReactomeiR-MMU-917729 Endosomal Sorting Complex Required For Transport (ESCRT)

Names & Taxonomyi

Protein namesi
Recommended name:
Tumor susceptibility gene 101 protein
Alternative name(s):
ESCRT-I complex subunit TSG101
Gene namesi
Name:Tsg101
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:106581 Tsg101

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Endosome, Membrane, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00000826072 – 391Tumor susceptibility gene 101 proteinAdd BLAST390

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Modified residuei221PhosphothreonineBy similarity1

Post-translational modificationi

Monoubiquitinated at multiple sites by LRSAM1 and by MGRN1. Ubiquitination inactivates it, possibly by regulating its shuttling between an active membrane-bound protein and an inactive soluble form. Ubiquitination by MGRN1 requires the presence of UBE2D1.By similarity

Keywords - PTMi

Acetylation, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ61187
MaxQBiQ61187
PaxDbiQ61187
PRIDEiQ61187

PTM databases

iPTMnetiQ61187
PhosphoSitePlusiQ61187

Expressioni

Tissue specificityi

Ubiquitous. Higher expression in brain and mammary gland. Lower expression in liver and tumoral tissues.

Developmental stagei

Expressed at all stages of mammary gland development, but at lower rate at early and mid pregnancy. Expressed in 1-cell and 2-cell stage embryos.

Gene expression databases

BgeeiENSMUSG00000014402
CleanExiMM_TSG101
ExpressionAtlasiQ61187 baseline and differential
GenevisibleiQ61187 MM

Interactioni

Subunit structurei

Component of the ESCRT-I complex (endosomal sorting complex required for transport I) which consists of TSG101, VPS28, a VPS37 protein (VPS37A to -D) and MVB12A or MVB12B in a 1:1:1:1 stoichiometry. Interacts with VPS37A, VPS37B and VPS37C. Component of an ESCRT-I complex (endosomal sorting complex required for transport I) which consists of TSG101, VPS28, VPS37A and UBAP1 in a 1:1:1:1 stoichiometry. Interacts with DMAP1 (PubMed:10888872). Interacts with GMCL (PubMed:12927808). Interacts with ubiquitin, stathmin and AATF (By similarity). Interacts with HGS; the interaction mediates the association with the ESCRT-0 complex (PubMed:12802020). Interacts with GGA1 and GGA3. Interacts (via UEV domain) with PDCD6IP/AIP1. Interacts with VPS28, SNF8 and VPS36. Self-associates. Interacts with MVB12A; the association appears to be mediated by the TSG101-VPS37 binary subcomplex. Interacts with VPS37D. Interacts with LRSAM1. Interacts with CEP55; the interaction is required for cytokinesis (By similarity). Interacts with PDCD6. Interacts with LITAF (By similarity). Interacts with murine leukemia virus Gag polyprotein (via PSAP motif) (PubMed:15908698). Interacts with MGRN1 (PubMed:19703557). Interacts with ARRDC1; recruits TSG101 to the plasma membrane (By similarity).By similarity5 Publications

GO - Molecular functioni

Protein-protein interaction databases

BioGridi204341, 30 interactors
ELMiQ61187
IntActiQ61187, 21 interactors
MINTiQ61187
STRINGi10090.ENSMUSP00000014546

Structurei

3D structure databases

ProteinModelPortaliQ61187
SMRiQ61187
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini2 – 145UEVPROSITE-ProRule annotationAdd BLAST144
Domaini323 – 391SBPROSITE-ProRule annotationAdd BLAST69

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni159 – 163Interaction with CEP55By similarity5

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili237 – 317Sequence analysisAdd BLAST81

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi321 – 324PTAP/PSAP motif4

Domaini

The UEV domain is required for the interaction of the complex with ubiquitin.
The coiled coil domain may interact with stathmin.

Sequence similaritiesi

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG2391 Eukaryota
ENOG410XT2Q LUCA
GeneTreeiENSGT00530000064004
HOGENOMiHOG000247008
HOVERGENiHBG057450
InParanoidiQ61187
KOiK12183
OMAiDHNGKVY
OrthoDBiEOG091G0HRA
PhylomeDBiQ61187
TreeFamiTF312917

Family and domain databases

Gene3Di3.10.110.10, 1 hit
InterProiView protein in InterPro
IPR037202 ESCRT_assembly_dom
IPR017916 SB_dom
IPR016135 UBQ-conjugating_enzyme/RWD
IPR008883 UEV_N
PfamiView protein in Pfam
PF05743 UEV, 1 hit
PF09454 Vps23_core, 1 hit
SUPFAMiSSF140111 SSF140111, 1 hit
SSF54495 SSF54495, 1 hit
PROSITEiView protein in PROSITE
PS51312 SB, 1 hit
PS51322 UEV, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q61187-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAVSESQLKK MMSKYKYRDL TVRQTVNVIA MYKDLKPVLD SYVFNDGSSR
60 70 80 90 100
ELVNLTGTIP VRYRGNIYNI PICLWLLDTY PYNPPICFVK PTSSMTIKTG
110 120 130 140 150
KHVDANGKIY LPYLHDWKHP RSELLELIQI MIVIFGEEPP VFSRPTVSAS
160 170 180 190 200
YPPYTATGPP NTSYMPGMPS GISAYPSGYP PNPSGYPGCP YPPAGPYPAT
210 220 230 240 250
TSSQYPSQPP VTTVGPSRDG TISEDTIRAS LISAVSDKLR WRMKEEMDGA
260 270 280 290 300
QAELNALKRT EEDLKKGHQK LEEMVTRLDQ EVAEVDKNIE LLKKKDEELS
310 320 330 340 350
SALEKMENQS ENNDIDEVII PTAPLYKQIL NLYAEENAIE DTIFYLGEAL
360 370 380 390
RRGVIDLDVF LKHVRLLSRK QFQLRALMQK ARKTAGLSDL Y
Length:391
Mass (Da):44,124
Last modified:August 1, 1998 - v2
Checksum:i79558EC535093492
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U52945 mRNA Translation: AAC53586.1
AF060868 Genomic DNA Translation: AAC83576.1
BC005424 mRNA Translation: AAH05424.1
BC085308 mRNA Translation: AAH85308.1
CCDSiCCDS21291.1
RefSeqiNP_068684.1, NM_021884.4
UniGeneiMm.241334

Genome annotation databases

EnsembliENSMUST00000014546; ENSMUSP00000014546; ENSMUSG00000014402
GeneIDi22088
KEGGimmu:22088
UCSCiuc009gzq.1 mouse

Similar proteinsi

Entry informationi

Entry nameiTS101_MOUSE
AccessioniPrimary (citable) accession number: Q61187
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: August 1, 1998
Last modified: May 23, 2018
This is version 178 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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