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Protein

Poly(A) polymerase alpha

Gene

Papola

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Polymerase that creates the 3'-poly(A) tail of mRNA's. Also required for the endoribonucleolytic cleavage reaction at some polyadenylation sites. May acquire specificity through interaction with a cleavage and polyadenylation specificity factor (CPSF) at its C-terminus.By similarity2 Publications

Catalytic activityi

ATP + RNA(n) = diphosphate + RNA(n+1).

Cofactori

Mg2+By similarity, Mn2+By similarityNote: Binds 2 magnesium ions. Also active with manganese.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei109ATPBy similarity1
Metal bindingi113Magnesium 1; catalyticBy similarity1
Metal bindingi113Magnesium 2; catalyticBy similarity1
Metal bindingi115Magnesium 1; catalyticBy similarity1
Metal bindingi115Magnesium 2; catalyticBy similarity1
Metal bindingi167Magnesium 2; catalyticBy similarity1
Binding sitei167ATPBy similarity1
Binding sitei228ATPBy similarity1
Binding sitei237ATPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi100 – 102ATPBy similarity3
Nucleotide bindingi113 – 115ATPBy similarity3
Nucleotide bindingi246 – 247ATPBy similarity2

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

mRNA processing

Keywords - Ligandi

ATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding, RNA-binding

Enzyme and pathway databases

ReactomeiR-MMU-109688. Cleavage of Growing Transcript in the Termination Region.
R-MMU-72163. mRNA Splicing - Major Pathway.
R-MMU-72187. mRNA 3'-end processing.
R-MMU-77595. Processing of Intronless Pre-mRNAs.

Names & Taxonomyi

Protein namesi
Recommended name:
Poly(A) polymerase alpha (EC:2.7.7.19)
Short name:
PAP-alpha
Alternative name(s):
Polynucleotide adenylyltransferase
Gene namesi
Name:Papola
Synonyms:Pap, Plap
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 12

Organism-specific databases

MGIiMGI:109301. Papola.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi444 – 445KK → RR: Some loss of sumoylation. No change in nuclear localization. 1 Publication2
Mutagenesisi537S → A: Eliminates MAPK-mediated phosphorylation. 1 Publication1
Mutagenesisi656 – 657KK → RR: Some loss of sumoylation; Largely localized to the cytoplasm. 1 Publication2

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00000516132 – 739Poly(A) polymerase alphaAdd BLAST738

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei10PhosphoserineBy similarity1
Modified residuei24PhosphoserineCombined sources1
Cross-linki444Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)1 Publication
Cross-linki445Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)1 Publication
Cross-linki506Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)1 Publication
Cross-linki507Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)1 Publication
Modified residuei537Phosphoserine; by MAPK1 Publication1
Modified residuei558PhosphoserineBy similarity1
Modified residuei635N6-acetyllysineBy similarity1
Modified residuei644N6-acetyllysineBy similarity1
Modified residuei730N6-acetyllysine; alternateBy similarity1
Cross-linki730Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO); alternate
Modified residuei732PhosphoserineBy similarity1
Modified residuei734N6-acetyllysine; alternateBy similarity1
Cross-linki734Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO); alternate

Post-translational modificationi

Polysumoylated. Varying sumolyation depending on tissue- and cell-type. Highly sumoylated in bladder and NIH 3T3 cells. Sumoylation is required for nuclear localization and enhances PAP stability. Desumoylated by SENP1. Inhibits polymerase activity.1 Publication
Hyperphosphorylation on multiple CDK2 consensus and non-consensus sites in the C-terminal Ser/Thr-rich region represses PAP activity in late M-phase. Phosphorylation/dephosphorylation may regulate the interaction between PAP and CPSF (By similarity).By similarity
Acetylated in the C-terminus. Acetylation decreases interaction with NUDT21 and KPNB1, and inhibits nuclear localization through inhibiting binding to the importin alpha/beta complex (By similarity).By similarity

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ61183.
PaxDbiQ61183.
PeptideAtlasiQ61183.
PRIDEiQ61183.

PTM databases

iPTMnetiQ61183.
PhosphoSitePlusiQ61183.

Expressioni

Tissue specificityi

Expressed in brain, thymus, lung, kidney, bladder, testis and spleen.1 Publication

Gene expression databases

BgeeiENSMUSG00000021111.
CleanExiMM_PAPOLA.
ExpressionAtlasiQ61183. baseline and differential.
GenevisibleiQ61183. MM.

Interactioni

Subunit structurei

Monomer. Found in a complex with CPSF1, FIP1L1 and PAPOLA. Interacts with AHCYL1 and FIP1L1; the interaction with AHCYL1 seems to increase interaction with FIP1L1 (PubMed:19224921). Interacts with NUDT21; the interaction is diminished by acetylation. Interacts with KPNB1; the interaction promotes PAP nuclear import and is inhibited by acetylation of PAP (By similarity).By similarity1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei153Interaction with RNABy similarity1
Sitei158Interaction with RNABy similarity1
Sitei328Interaction with RNABy similarity1
Sitei399Interaction with RNABy similarity1
Sitei524Interaction with RNABy similarity1

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000021535.

Structurei

3D structure databases

ProteinModelPortaliQ61183.
SMRiQ61183.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni508 – 643Ser/Thr-richAdd BLAST136
Regioni671 – 739Required for interaction with NUDT211 PublicationAdd BLAST69

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi490 – 507Nuclear localization signal 1By similarityAdd BLAST18
Motifi644 – 659Nuclear localization signal 2By similarityAdd BLAST16

Sequence similaritiesi

Belongs to the poly(A) polymerase family.Curated

Phylogenomic databases

eggNOGiKOG2245. Eukaryota.
COG5186. LUCA.
GeneTreeiENSGT00390000017928.
HOGENOMiHOG000204376.
HOVERGENiHBG053502.
InParanoidiQ61183.
KOiK14376.
OMAiDMKIAAR.
OrthoDBiEOG091G0571.
PhylomeDBiQ61183.
TreeFamiTF300842.

Family and domain databases

Gene3Di3.30.70.590. 1 hit.
InterProiIPR011068. NuclTrfase_I_C.
IPR007012. PolA_pol_cen_dom.
IPR007010. PolA_pol_RNA-bd_dom.
IPR014492. PolyA_polymerase.
IPR002934. Polymerase_NTP_transf_dom.
[Graphical view]
PANTHERiPTHR10682. PTHR10682. 1 hit.
PfamiPF01909. NTP_transf_2. 1 hit.
PF04928. PAP_central. 1 hit.
PF04926. PAP_RNA-bind. 1 hit.
[Graphical view]
PIRSFiPIRSF018425. PolyA_polymerase. 1 hit.
SUPFAMiSSF55003. SSF55003. 1 hit.

Sequences (4)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q61183-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MPFPVTTQGS QQTQPPQRHY GITSPISLAA PKETDCLLTQ KLIETLKPFG
60 70 80 90 100
VFEEEEELQR RILILGKLNN LVKEWIREIS ESKNLPQSVI ENVGGKIFTF
110 120 130 140 150
GSYRLGVHTK GADIDALCVA PRHVDRSDFF TSFYDKLKLQ EEVKDLRAVE
160 170 180 190 200
EAFVPVIKLC FDGIEIDILF ARLALQTIPE DLDLRDDSLL KNLDIRCIRS
210 220 230 240 250
LNGCRVTDEI LHLVPNIDNF RLTLRAIKLW AKRHNIYSNI LGFLGGVSWA
260 270 280 290 300
MLVARTCQLY PNAIASTLVH KFFLVFSKWE WPNPVLLKQP EECNLNLPVW
310 320 330 340 350
DPRVNPSDRY HLMPIITPAY PQQNSTYNVS VSTRMVMVEE FKQGLAITDE
360 370 380 390 400
ILLSKAEWSK LFEAPNFFQK YKHYIVLLAS APTEKQRLEW VGLVESKIRI
410 420 430 440 450
LVGSLEKNEF ITLAHVNPQS FPAPKESPDR EEFRTMWVIG LVFKKTENSE
460 470 480 490 500
NLSVDLTYDI QSFTDTVYRQ AINSKMFELD MKIAAMHVKR KQLHQLLPSH
510 520 530 540 550
VLQKRKKHST EGVKLTALND SSLDLSMDSD NSMSVPSPTS AMKTSPLNSS
560 570 580 590 600
GSSQGRNSPA PAVTAASVTS IQASEVSVPQ ANSSESPGGP SSESIPQTAT
610 620 630 640 650
QPAISPPPKP TVSRVVSSTR LVNPSPRPSG NTATKVPNPI VGVKRTSSPN
660 670 680 690 700
KEESPKKTKT EEDETSEDAN CLALSGHDKT ETKEQVDLET SAVQSETVPA
710 720 730
SASLLASQKT SSTDLSDIPA LPANPIPVIK NSIKLRLNR
Length:739
Mass (Da):82,309
Last modified:January 23, 2007 - v4
Checksum:iBABE406BDF8EBEE9
GO
Isoform 2 (identifier: Q61183-2) [UniParc]FASTAAdd to basket
Also known as: III

The sequence of this isoform differs from the canonical sequence as follows:
     373-375: HYI → YVK
     376-739: Missing.

Show »
Length:375
Mass (Da):43,110
Checksum:i09C195A8E639A0B2
GO
Isoform 3 (identifier: Q61183-3) [UniParc]FASTAAdd to basket
Also known as: V

The sequence of this isoform differs from the canonical sequence as follows:
     305-317: NPSDRYHLMPIIT → SVLFFPLQIHTIQ
     318-375: Missing.

Show »
Length:681
Mass (Da):75,495
Checksum:iAA7913AC868F72CF
GO
Isoform 4 (identifier: Q61183-4) [UniParc]FASTAAdd to basket
Also known as: VI

The sequence of this isoform differs from the canonical sequence as follows:
     280-293: EWPNPVLLKQPEEC → YVFRLYYNKIDCRH
     294-375: Missing.

Show »
Length:657
Mass (Da):72,874
Checksum:i8EE01C27FBF14541
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti47K → L in AAC52586 (PubMed:8628305).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_004527280 – 293EWPNP…QPEEC → YVFRLYYNKIDCRH in isoform 4. 1 PublicationAdd BLAST14
Alternative sequenceiVSP_004528294 – 375Missing in isoform 4. 1 PublicationAdd BLAST82
Alternative sequenceiVSP_004529305 – 317NPSDR…MPIIT → SVLFFPLQIHTIQ in isoform 3. 1 PublicationAdd BLAST13
Alternative sequenceiVSP_004530318 – 375Missing in isoform 3. 1 PublicationAdd BLAST58
Alternative sequenceiVSP_004531373 – 375HYI → YVK in isoform 2. 1 Publication3
Alternative sequenceiVSP_004532376 – 739Missing in isoform 2. 1 PublicationAdd BLAST364

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U52197 mRNA. Translation: AAC52586.1.
U58134 mRNA. Translation: AAC52608.1.
U58135 mRNA. Translation: AAC52609.1.
AB086650 Genomic DNA. Translation: BAC00996.1.
CCDSiCCDS49163.1. [Q61183-1]
RefSeqiNP_035242.1. NM_011112.3. [Q61183-1]
UniGeneiMm.255877.

Genome annotation databases

EnsembliENSMUST00000021535; ENSMUSP00000021535; ENSMUSG00000021111. [Q61183-1]
ENSMUST00000109901; ENSMUSP00000105527; ENSMUSG00000021111. [Q61183-1]
GeneIDi18789.
KEGGimmu:18789.
UCSCiuc007oyv.1. mouse. [Q61183-2]
uc007oyz.2. mouse. [Q61183-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U52197 mRNA. Translation: AAC52586.1.
U58134 mRNA. Translation: AAC52608.1.
U58135 mRNA. Translation: AAC52609.1.
AB086650 Genomic DNA. Translation: BAC00996.1.
CCDSiCCDS49163.1. [Q61183-1]
RefSeqiNP_035242.1. NM_011112.3. [Q61183-1]
UniGeneiMm.255877.

3D structure databases

ProteinModelPortaliQ61183.
SMRiQ61183.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000021535.

PTM databases

iPTMnetiQ61183.
PhosphoSitePlusiQ61183.

Proteomic databases

EPDiQ61183.
PaxDbiQ61183.
PeptideAtlasiQ61183.
PRIDEiQ61183.

Protocols and materials databases

DNASUi18789.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000021535; ENSMUSP00000021535; ENSMUSG00000021111. [Q61183-1]
ENSMUST00000109901; ENSMUSP00000105527; ENSMUSG00000021111. [Q61183-1]
GeneIDi18789.
KEGGimmu:18789.
UCSCiuc007oyv.1. mouse. [Q61183-2]
uc007oyz.2. mouse. [Q61183-1]

Organism-specific databases

CTDi10914.
MGIiMGI:109301. Papola.

Phylogenomic databases

eggNOGiKOG2245. Eukaryota.
COG5186. LUCA.
GeneTreeiENSGT00390000017928.
HOGENOMiHOG000204376.
HOVERGENiHBG053502.
InParanoidiQ61183.
KOiK14376.
OMAiDMKIAAR.
OrthoDBiEOG091G0571.
PhylomeDBiQ61183.
TreeFamiTF300842.

Enzyme and pathway databases

ReactomeiR-MMU-109688. Cleavage of Growing Transcript in the Termination Region.
R-MMU-72163. mRNA Splicing - Major Pathway.
R-MMU-72187. mRNA 3'-end processing.
R-MMU-77595. Processing of Intronless Pre-mRNAs.

Miscellaneous databases

ChiTaRSiPapola. mouse.
PROiQ61183.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000021111.
CleanExiMM_PAPOLA.
ExpressionAtlasiQ61183. baseline and differential.
GenevisibleiQ61183. MM.

Family and domain databases

Gene3Di3.30.70.590. 1 hit.
InterProiIPR011068. NuclTrfase_I_C.
IPR007012. PolA_pol_cen_dom.
IPR007010. PolA_pol_RNA-bd_dom.
IPR014492. PolyA_polymerase.
IPR002934. Polymerase_NTP_transf_dom.
[Graphical view]
PANTHERiPTHR10682. PTHR10682. 1 hit.
PfamiPF01909. NTP_transf_2. 1 hit.
PF04928. PAP_central. 1 hit.
PF04926. PAP_RNA-bind. 1 hit.
[Graphical view]
PIRSFiPIRSF018425. PolyA_polymerase. 1 hit.
SUPFAMiSSF55003. SSF55003. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiPAPOA_MOUSE
AccessioniPrimary (citable) accession number: Q61183
Secondary accession number(s): Q61208, Q61209, Q8K4X2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 15, 2002
Last sequence update: January 23, 2007
Last modified: November 2, 2016
This is version 131 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.