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Protein

Interferon regulatory factor 9

Gene

Irf9

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcription factor that mediates signaling by type I IFNs (IFN-alpha and IFN-beta). Following type I IFN binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. IRF9/ISGF3G associateswith the phosphorylated STAT1:STAT2 dimer to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of interferon stimulated genes, which drive the cell in an antiviral state.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi9 – 116108IRF tryptophan pentad repeatPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. regulatory region DNA binding Source: InterPro
  2. sequence-specific DNA binding transcription factor activity Source: InterPro

GO - Biological processi

  1. transcription, DNA-templated Source: UniProtKB-KW
  2. type I interferon biosynthetic process Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

ReactomeiREACT_336467. Interferon alpha/beta signaling.
REACT_349056. Interferon gamma signaling.

Names & Taxonomyi

Protein namesi
Recommended name:
Interferon regulatory factor 9
Short name:
IRF-9
Alternative name(s):
IFN-alpha-responsive transcription factor subunit
ISGF3 p48 subunit
Interferon-stimulated gene factor 3 gamma
Short name:
ISGF-3 gamma
Transcriptional regulator ISGF3 subunit gamma
Gene namesi
Name:Irf9
Synonyms:Isgf3g
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 14

Organism-specific databases

MGIiMGI:107587. Irf9.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: MGI
  2. nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 399399Interferon regulatory factor 9PRO_0000154568Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei393 – 3931Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ61179.
PaxDbiQ61179.
PRIDEiQ61179.

PTM databases

PhosphoSiteiQ61179.

Expressioni

Inductioni

By IFN-alpha and IFN-beta. Upon stimulation the regulatory phosphorylated alpha and beta subunits assemble with the gamma subunit and translocate from the cytoplasm to the nucleus.

Gene expression databases

BgeeiQ61179.
CleanExiMM_IRF9.
ExpressionAtlasiQ61179. baseline and differential.
GenevestigatoriQ61179.

Interactioni

Subunit structurei

Interacts with STAT2 in the cytoplasm. Forms the interferon-stimulated gene factor 3 complex (ISGF3) with the heterodimer STAT1:STAT2; upon stimulation.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
Stat2Q9QXJ29EBI-646653,EBI-646643

Protein-protein interaction databases

BioGridi200811. 8 interactions.
IntActiQ61179. 2 interactions.
STRINGi10090.ENSMUSP00000120525.

Structurei

3D structure databases

ProteinModelPortaliQ61179.
SMRiQ61179. Positions 11-116, 204-379.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi165 – 1706Poly-Gly

Sequence similaritiesi

Belongs to the IRF family.PROSITE-ProRule annotation
Contains 1 IRF tryptophan pentad repeat DNA-binding domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiNOG43166.
GeneTreeiENSGT00760000119093.
HOGENOMiHOG000010107.
HOVERGENiHBG003072.
InParanoidiQ61179.
KOiK04693.
PhylomeDBiQ61179.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
2.60.200.10. 1 hit.
InterProiIPR019817. Interferon_reg_fac_CS.
IPR001346. Interferon_reg_fact_DNA-bd_dom.
IPR019471. Interferon_reg_factor-3.
IPR017855. SMAD_dom-like.
IPR008984. SMAD_FHA_domain.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00605. IRF. 1 hit.
PF10401. IRF-3. 1 hit.
[Graphical view]
PRINTSiPR00267. INTFRNREGFCT.
SMARTiSM00348. IRF. 1 hit.
[Graphical view]
SUPFAMiSSF49879. SSF49879. 1 hit.
PROSITEiPS00601. IRF_1. 1 hit.
PS51507. IRF_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q61179-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASGKVRCTR KLRSWIVEQV ESGHFPGVCW DDAAKTMFRI PWKHAGKQDF
60 70 80 90 100
REDQDAAIFK AWALFKEKHK DGDIGHPAVW KTRLRCALNK SSEFEEVPER
110 120 130 140 150
GRMDVAEPYK VYRILPAGTL PNQPRNQKSP CKRSISCVSP EREENMENGR
160 170 180 190 200
TNGVVNHSDS GSNIGGGGNG SNRSDSNSNC NSELEEGAGT TEATIREDPV
210 220 230 240 250
FLEHQLPLNS DYSLLLTFIY GGRVVGKTQV HSLDCRLVAE RSDSESSMEQ
260 270 280 290 300
VEFPKPDPLE PTQHLLNQLD RGVLVASNSR GLFVQRLCPI PISWNAPEAP
310 320 330 340 350
PGPGPHLLPS NKCVELFKTT YFCRDLAQYF QGQGPPPKFQ ATLHFWEESP
360 370 380 390
GSSHSQENLI TVQMEQAFAR HLLEKIPEEE KAALFLLQHT EQSPSALGH
Length:399
Mass (Da):44,610
Last modified:November 1, 1997 - v1
Checksum:i92F9481ACEB669BD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U51992 mRNA. Translation: AAC52494.1.
BC005435 mRNA. Translation: AAH05435.1.
BC012968 mRNA. Translation: AAH12968.1.
CCDSiCCDS27119.1.
PIRiJC4592.
RefSeqiNP_001152889.1. NM_001159417.1.
NP_001152890.1. NM_001159418.1.
NP_032420.1. NM_008394.3.
UniGeneiMm.2032.

Genome annotation databases

EnsembliENSMUST00000130697; ENSMUSP00000120359; ENSMUSG00000002325.
GeneIDi16391.
KEGGimmu:16391.
UCSCiuc007tzl.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U51992 mRNA. Translation: AAC52494.1.
BC005435 mRNA. Translation: AAH05435.1.
BC012968 mRNA. Translation: AAH12968.1.
CCDSiCCDS27119.1.
PIRiJC4592.
RefSeqiNP_001152889.1. NM_001159417.1.
NP_001152890.1. NM_001159418.1.
NP_032420.1. NM_008394.3.
UniGeneiMm.2032.

3D structure databases

ProteinModelPortaliQ61179.
SMRiQ61179. Positions 11-116, 204-379.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi200811. 8 interactions.
IntActiQ61179. 2 interactions.
STRINGi10090.ENSMUSP00000120525.

PTM databases

PhosphoSiteiQ61179.

Proteomic databases

MaxQBiQ61179.
PaxDbiQ61179.
PRIDEiQ61179.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000130697; ENSMUSP00000120359; ENSMUSG00000002325.
GeneIDi16391.
KEGGimmu:16391.
UCSCiuc007tzl.2. mouse.

Organism-specific databases

CTDi10379.
MGIiMGI:107587. Irf9.

Phylogenomic databases

eggNOGiNOG43166.
GeneTreeiENSGT00760000119093.
HOGENOMiHOG000010107.
HOVERGENiHBG003072.
InParanoidiQ61179.
KOiK04693.
PhylomeDBiQ61179.

Enzyme and pathway databases

ReactomeiREACT_336467. Interferon alpha/beta signaling.
REACT_349056. Interferon gamma signaling.

Miscellaneous databases

ChiTaRSiIrf9. mouse.
NextBioi289533.
PROiQ61179.
SOURCEiSearch...

Gene expression databases

BgeeiQ61179.
CleanExiMM_IRF9.
ExpressionAtlasiQ61179. baseline and differential.
GenevestigatoriQ61179.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
2.60.200.10. 1 hit.
InterProiIPR019817. Interferon_reg_fac_CS.
IPR001346. Interferon_reg_fact_DNA-bd_dom.
IPR019471. Interferon_reg_factor-3.
IPR017855. SMAD_dom-like.
IPR008984. SMAD_FHA_domain.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00605. IRF. 1 hit.
PF10401. IRF-3. 1 hit.
[Graphical view]
PRINTSiPR00267. INTFRNREGFCT.
SMARTiSM00348. IRF. 1 hit.
[Graphical view]
SUPFAMiSSF49879. SSF49879. 1 hit.
PROSITEiPS00601. IRF_1. 1 hit.
PS51507. IRF_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Structure of mouse interferon stimulated gene factor 3 gamma (ISGF3 gamma/p48) cDNA and chromosomal localization of the gene."
    Suhara W., Yoneyama M., Yonekawa H., Takashi F.
    J. Biochem. 119:231-234(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Mammary gland.
  3. "Possible involvement of the transcription factor ISGF3 gamma in virus-induced expression of the IFN-beta gene."
    Kawakami T., Matsumoto M., Sato M., Harada H., Taniguchi T., Kitagawa M.
    FEBS Lett. 358:225-229(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-87.
  4. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-393, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.
  5. "Multiple functions of the IKK-related kinase IKKepsilon in interferon-mediated antiviral immunity."
    Tenoever B.R., Ng S.L., Chua M.A., McWhirter S.M., Garcia-Sastre A., Maniatis T.
    Science 315:1274-1278(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN THE ISGF3 COMPLEX.

Entry informationi

Entry nameiIRF9_MOUSE
AccessioniPrimary (citable) accession number: Q61179
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: April 1, 2015
This is version 124 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.