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Q61179

- IRF9_MOUSE

UniProt

Q61179 - IRF9_MOUSE

Protein

Interferon regulatory factor 9

Gene

Irf9

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 119 (01 Oct 2014)
      Sequence version 1 (01 Nov 1997)
      Previous versions | rss
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    Functioni

    Transcription factor that mediates signaling by type I IFNs (IFN-alpha and IFN-beta). Following type I IFN binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. IRF9/ISGF3G associateswith the phosphorylated STAT1:STAT2 dimer to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of interferon stimulated genes, which drive the cell in an antiviral state.

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    DNA bindingi9 – 116108IRF tryptophan pentad repeatPROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. protein binding Source: IntAct
    2. regulatory region DNA binding Source: InterPro
    3. sequence-specific DNA binding transcription factor activity Source: InterPro

    GO - Biological processi

    1. transcription, DNA-templated Source: UniProtKB-KW
    2. type I interferon biosynthetic process Source: MGI

    Keywords - Molecular functioni

    Activator

    Keywords - Biological processi

    Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding

    Enzyme and pathway databases

    ReactomeiREACT_198622. Interferon alpha/beta signaling.
    REACT_198660. Interferon gamma signaling.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Interferon regulatory factor 9
    Short name:
    IRF-9
    Alternative name(s):
    IFN-alpha-responsive transcription factor subunit
    ISGF3 p48 subunit
    Interferon-stimulated gene factor 3 gamma
    Short name:
    ISGF-3 gamma
    Transcriptional regulator ISGF3 subunit gamma
    Gene namesi
    Name:Irf9
    Synonyms:Isgf3g
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 14

    Organism-specific databases

    MGIiMGI:107587. Irf9.

    Subcellular locationi

    GO - Cellular componenti

    1. nucleus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 399399Interferon regulatory factor 9PRO_0000154568Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei393 – 3931Phosphoserine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiQ61179.
    PRIDEiQ61179.

    PTM databases

    PhosphoSiteiQ61179.

    Expressioni

    Inductioni

    By IFN-alpha and IFN-beta. Upon stimulation the regulatory phosphorylated alpha and beta subunits assemble with the gamma subunit and translocate from the cytoplasm to the nucleus.

    Gene expression databases

    ArrayExpressiQ61179.
    BgeeiQ61179.
    CleanExiMM_IRF9.
    GenevestigatoriQ61179.

    Interactioni

    Subunit structurei

    Interacts with STAT2 in the cytoplasm. Forms the interferon-stimulated gene factor 3 complex (ISGF3) with the heterodimer STAT1:STAT2; upon stimulation.1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    Stat2Q9QXJ29EBI-646653,EBI-646643

    Protein-protein interaction databases

    BioGridi200811. 8 interactions.
    IntActiQ61179. 2 interactions.
    STRINGi10090.ENSMUSP00000120525.

    Structurei

    3D structure databases

    ProteinModelPortaliQ61179.
    SMRiQ61179. Positions 11-116, 204-379.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi165 – 1706Poly-Gly

    Sequence similaritiesi

    Belongs to the IRF family.PROSITE-ProRule annotation
    Contains 1 IRF tryptophan pentad repeat DNA-binding domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiNOG43166.
    GeneTreeiENSGT00740000114956.
    HOGENOMiHOG000010107.
    HOVERGENiHBG003072.
    InParanoidiQ61179.
    KOiK04693.
    PhylomeDBiQ61179.

    Family and domain databases

    Gene3Di1.10.10.10. 1 hit.
    2.60.200.10. 1 hit.
    InterProiIPR019817. Interferon_reg_fac_CS.
    IPR001346. Interferon_reg_fact_DNA-bd_dom.
    IPR019471. Interferon_reg_factor-3.
    IPR017855. SMAD_dom-like.
    IPR008984. SMAD_FHA_domain.
    IPR011991. WHTH_DNA-bd_dom.
    [Graphical view]
    PfamiPF00605. IRF. 1 hit.
    PF10401. IRF-3. 1 hit.
    [Graphical view]
    PRINTSiPR00267. INTFRNREGFCT.
    SMARTiSM00348. IRF. 1 hit.
    [Graphical view]
    SUPFAMiSSF49879. SSF49879. 1 hit.
    PROSITEiPS00601. IRF_1. 1 hit.
    PS51507. IRF_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q61179-1 [UniParc]FASTAAdd to Basket

    « Hide

    MASGKVRCTR KLRSWIVEQV ESGHFPGVCW DDAAKTMFRI PWKHAGKQDF    50
    REDQDAAIFK AWALFKEKHK DGDIGHPAVW KTRLRCALNK SSEFEEVPER 100
    GRMDVAEPYK VYRILPAGTL PNQPRNQKSP CKRSISCVSP EREENMENGR 150
    TNGVVNHSDS GSNIGGGGNG SNRSDSNSNC NSELEEGAGT TEATIREDPV 200
    FLEHQLPLNS DYSLLLTFIY GGRVVGKTQV HSLDCRLVAE RSDSESSMEQ 250
    VEFPKPDPLE PTQHLLNQLD RGVLVASNSR GLFVQRLCPI PISWNAPEAP 300
    PGPGPHLLPS NKCVELFKTT YFCRDLAQYF QGQGPPPKFQ ATLHFWEESP 350
    GSSHSQENLI TVQMEQAFAR HLLEKIPEEE KAALFLLQHT EQSPSALGH 399
    Length:399
    Mass (Da):44,610
    Last modified:November 1, 1997 - v1
    Checksum:i92F9481ACEB669BD
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U51992 mRNA. Translation: AAC52494.1.
    BC005435 mRNA. Translation: AAH05435.1.
    BC012968 mRNA. Translation: AAH12968.1.
    CCDSiCCDS27119.1.
    PIRiJC4592.
    RefSeqiNP_001152889.1. NM_001159417.1.
    NP_001152890.1. NM_001159418.1.
    NP_032420.1. NM_008394.3.
    UniGeneiMm.2032.

    Genome annotation databases

    EnsembliENSMUST00000130697; ENSMUSP00000120359; ENSMUSG00000002325.
    GeneIDi16391.
    KEGGimmu:16391.
    UCSCiuc007tzl.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U51992 mRNA. Translation: AAC52494.1 .
    BC005435 mRNA. Translation: AAH05435.1 .
    BC012968 mRNA. Translation: AAH12968.1 .
    CCDSi CCDS27119.1.
    PIRi JC4592.
    RefSeqi NP_001152889.1. NM_001159417.1.
    NP_001152890.1. NM_001159418.1.
    NP_032420.1. NM_008394.3.
    UniGenei Mm.2032.

    3D structure databases

    ProteinModelPortali Q61179.
    SMRi Q61179. Positions 11-116, 204-379.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 200811. 8 interactions.
    IntActi Q61179. 2 interactions.
    STRINGi 10090.ENSMUSP00000120525.

    PTM databases

    PhosphoSitei Q61179.

    Proteomic databases

    PaxDbi Q61179.
    PRIDEi Q61179.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000130697 ; ENSMUSP00000120359 ; ENSMUSG00000002325 .
    GeneIDi 16391.
    KEGGi mmu:16391.
    UCSCi uc007tzl.2. mouse.

    Organism-specific databases

    CTDi 10379.
    MGIi MGI:107587. Irf9.

    Phylogenomic databases

    eggNOGi NOG43166.
    GeneTreei ENSGT00740000114956.
    HOGENOMi HOG000010107.
    HOVERGENi HBG003072.
    InParanoidi Q61179.
    KOi K04693.
    PhylomeDBi Q61179.

    Enzyme and pathway databases

    Reactomei REACT_198622. Interferon alpha/beta signaling.
    REACT_198660. Interferon gamma signaling.

    Miscellaneous databases

    ChiTaRSi IRF9. mouse.
    NextBioi 289533.
    PROi Q61179.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q61179.
    Bgeei Q61179.
    CleanExi MM_IRF9.
    Genevestigatori Q61179.

    Family and domain databases

    Gene3Di 1.10.10.10. 1 hit.
    2.60.200.10. 1 hit.
    InterProi IPR019817. Interferon_reg_fac_CS.
    IPR001346. Interferon_reg_fact_DNA-bd_dom.
    IPR019471. Interferon_reg_factor-3.
    IPR017855. SMAD_dom-like.
    IPR008984. SMAD_FHA_domain.
    IPR011991. WHTH_DNA-bd_dom.
    [Graphical view ]
    Pfami PF00605. IRF. 1 hit.
    PF10401. IRF-3. 1 hit.
    [Graphical view ]
    PRINTSi PR00267. INTFRNREGFCT.
    SMARTi SM00348. IRF. 1 hit.
    [Graphical view ]
    SUPFAMi SSF49879. SSF49879. 1 hit.
    PROSITEi PS00601. IRF_1. 1 hit.
    PS51507. IRF_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Structure of mouse interferon stimulated gene factor 3 gamma (ISGF3 gamma/p48) cDNA and chromosomal localization of the gene."
      Suhara W., Yoneyama M., Yonekawa H., Takashi F.
      J. Biochem. 119:231-234(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: FVB/N.
      Tissue: Mammary gland.
    3. "Possible involvement of the transcription factor ISGF3 gamma in virus-induced expression of the IFN-beta gene."
      Kawakami T., Matsumoto M., Sato M., Harada H., Taniguchi T., Kitagawa M.
      FEBS Lett. 358:225-229(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-87.
    4. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-393, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Liver.
    5. "Multiple functions of the IKK-related kinase IKKepsilon in interferon-mediated antiviral immunity."
      Tenoever B.R., Ng S.L., Chua M.A., McWhirter S.M., Garcia-Sastre A., Maniatis T.
      Science 315:1274-1278(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION IN THE ISGF3 COMPLEX.

    Entry informationi

    Entry nameiIRF9_MOUSE
    AccessioniPrimary (citable) accession number: Q61179
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: November 1, 1997
    Last modified: October 1, 2014
    This is version 119 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3