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Q61179 (IRF9_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 115. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Interferon regulatory factor 9

Short name=IRF-9
Alternative name(s):
IFN-alpha-responsive transcription factor subunit
ISGF3 p48 subunit
Interferon-stimulated gene factor 3 gamma
Short name=ISGF-3 gamma
Transcriptional regulator ISGF3 subunit gamma
Gene names
Name:Irf9
Synonyms:Isgf3g
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length399 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Transcription factor that mediates signaling by type I IFNs (IFN-alpha and IFN-beta). Following type I IFN binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. IRF9/ISGF3G associateswith the phosphorylated STAT1:STAT2 dimer to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of interferon stimulated genes, which drive the cell in an antiviral state.

Subunit structure

Interacts with STAT2 in the cytoplasm. Forms the interferon-stimulated gene factor 3 complex (ISGF3) with the heterodimer STAT1:STAT2; upon stimulation. Ref.5

Subcellular location

Nucleus.

Induction

By IFN-alpha and IFN-beta. Upon stimulation the regulatory phosphorylated alpha and beta subunits assemble with the gamma subunit and translocate from the cytoplasm to the nucleus.

Sequence similarities

Belongs to the IRF family.

Contains 1 IRF tryptophan pentad repeat DNA-binding domain.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Stat2Q9QXJ29EBI-646653,EBI-646643

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 399399Interferon regulatory factor 9
PRO_0000154568

Regions

DNA binding9 – 116108IRF tryptophan pentad repeat
Compositional bias165 – 1706Poly-Gly

Amino acid modifications

Modified residue3931Phosphoserine Ref.4

Sequences

Sequence LengthMass (Da)Tools
Q61179 [UniParc].

Last modified November 1, 1997. Version 1.
Checksum: 92F9481ACEB669BD

FASTA39944,610
        10         20         30         40         50         60 
MASGKVRCTR KLRSWIVEQV ESGHFPGVCW DDAAKTMFRI PWKHAGKQDF REDQDAAIFK 

        70         80         90        100        110        120 
AWALFKEKHK DGDIGHPAVW KTRLRCALNK SSEFEEVPER GRMDVAEPYK VYRILPAGTL 

       130        140        150        160        170        180 
PNQPRNQKSP CKRSISCVSP EREENMENGR TNGVVNHSDS GSNIGGGGNG SNRSDSNSNC 

       190        200        210        220        230        240 
NSELEEGAGT TEATIREDPV FLEHQLPLNS DYSLLLTFIY GGRVVGKTQV HSLDCRLVAE 

       250        260        270        280        290        300 
RSDSESSMEQ VEFPKPDPLE PTQHLLNQLD RGVLVASNSR GLFVQRLCPI PISWNAPEAP 

       310        320        330        340        350        360 
PGPGPHLLPS NKCVELFKTT YFCRDLAQYF QGQGPPPKFQ ATLHFWEESP GSSHSQENLI 

       370        380        390 
TVQMEQAFAR HLLEKIPEEE KAALFLLQHT EQSPSALGH 

« Hide

References

« Hide 'large scale' references
[1]"Structure of mouse interferon stimulated gene factor 3 gamma (ISGF3 gamma/p48) cDNA and chromosomal localization of the gene."
Suhara W., Yoneyama M., Yonekawa H., Takashi F.
J. Biochem. 119:231-234(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: FVB/N.
Tissue: Mammary gland.
[3]"Possible involvement of the transcription factor ISGF3 gamma in virus-induced expression of the IFN-beta gene."
Kawakami T., Matsumoto M., Sato M., Harada H., Taniguchi T., Kitagawa M.
FEBS Lett. 358:225-229(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-87.
[4]"Large-scale phosphorylation analysis of mouse liver."
Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-393, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Liver.
[5]"Multiple functions of the IKK-related kinase IKKepsilon in interferon-mediated antiviral immunity."
Tenoever B.R., Ng S.L., Chua M.A., McWhirter S.M., Garcia-Sastre A., Maniatis T.
Science 315:1274-1278(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION IN THE ISGF3 COMPLEX.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U51992 mRNA. Translation: AAC52494.1.
BC005435 mRNA. Translation: AAH05435.1.
BC012968 mRNA. Translation: AAH12968.1.
PIRJC4592.
RefSeqNP_001152889.1. NM_001159417.1.
NP_001152890.1. NM_001159418.1.
NP_032420.1. NM_008394.3.
UniGeneMm.2032.

3D structure databases

ProteinModelPortalQ61179.
SMRQ61179. Positions 11-116, 204-379.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid200811. 8 interactions.
IntActQ61179. 2 interactions.
STRING10090.ENSMUSP00000120525.

PTM databases

PhosphoSiteQ61179.

Proteomic databases

PaxDbQ61179.
PRIDEQ61179.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000130697; ENSMUSP00000120359; ENSMUSG00000002325.
GeneID16391.
KEGGmmu:16391.
UCSCuc007tzl.2. mouse.

Organism-specific databases

CTD10379.
MGIMGI:107587. Irf9.

Phylogenomic databases

eggNOGNOG43166.
GeneTreeENSGT00740000114956.
HOGENOMHOG000010107.
HOVERGENHBG003072.
InParanoidQ61179.
KOK04693.
PhylomeDBQ61179.

Gene expression databases

ArrayExpressQ61179.
BgeeQ61179.
CleanExMM_IRF9.
GenevestigatorQ61179.

Family and domain databases

Gene3D1.10.10.10. 1 hit.
2.60.200.10. 1 hit.
InterProIPR019817. Interferon_reg_fac_CS.
IPR001346. Interferon_reg_fact_DNA-bd_dom.
IPR019471. Interferon_reg_factor-3.
IPR017855. SMAD_dom-like.
IPR008984. SMAD_FHA_domain.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamPF00605. IRF. 1 hit.
PF10401. IRF-3. 1 hit.
[Graphical view]
PRINTSPR00267. INTFRNREGFCT.
SMARTSM00348. IRF. 1 hit.
[Graphical view]
SUPFAMSSF49879. SSF49879. 1 hit.
PROSITEPS00601. IRF_1. 1 hit.
PS51507. IRF_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSIRF9. mouse.
NextBio289533.
PROQ61179.
SOURCESearch...

Entry information

Entry nameIRF9_MOUSE
AccessionPrimary (citable) accession number: Q61179
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: April 16, 2014
This is version 115 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot