Reviewed, UniProtKB/Swiss-Prot Q61176 (ARGI1_MOUSE)

Names and origin

Protein names

Recommended name:
    Arginase-1
    EC=3.5.3.1
Alternative name(s):
    Type I arginase
    Liver-type arginase

Gene names

Name:

Arg1

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	323 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	L-arginine + H2O = L-ornithine + urea.
Cofactor	Binds 2 manganese ions per subunit By similarity.
Pathway	Nitrogen metabolism; urea cycle; L-ornithine and urea from L-arginine: step 1/1.
Subunit structure	Homotrimer By similarity.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the arginase family.

Ontologies

Keywords
Biological process	Arginine metabolism Urea cycle
Cellular component	Cytoplasm
Ligand	Manganese Metal-binding
Molecular function	Hydrolase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	arginine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW urea cycle Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	arginase activity Inferred from direct assay. Source: MGI manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 323	323	Arginase-1		PRO_0000173694
Regions
	Region	126 – 130	5	Substrate binding By similarity
	Region	137 – 139	3	Substrate binding By similarity
Sites
	Metal binding	101	1	Manganese 1 By similarity
	Metal binding	124	1	Manganese 1 By similarity
	Metal binding	124	1	Manganese 2 By similarity
	Metal binding	126	1	Manganese 2 By similarity
	Metal binding	128	1	Manganese 1 By similarity
	Metal binding	232	1	Manganese 1 By similarity
	Metal binding	232	1	Manganese 2 By similarity
	Metal binding	234	1	Manganese 2 By similarity
	Binding site	183	1	Substrate By similarity
Amino acid modifications
	Modified residue	3	1	Phosphoserine Ref.6
	Modified residue	230	1	Phosphoserine Ref.5
Experimental info
	Sequence conflict	109	1	S → T in BAE28901. Ref.2
	Sequence conflict	306	1	T → A in CAJ18381. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Q61176-1 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: 6D0231978AC1B977	FASTA	323	34,808
	10 20 30 40 50 60 MSSKPKSLEI IGAPFSKGQP RGGVEKGPAA LRKAGLLEKL KETEYDVRDH GDLAFVDVPN 70 80 90 100 110 120 DSSFQIVKNP RSVGKANEEL AGVVAEVQKN GRVSVVLGGD HSLAVGSISG HARVHPDLCV 130 140 150 160 170 180 IWVDAHTDIN TPLTTSSGNL HGQPVSFLLK ELKGKFPDVP GFSWVTPCIS AKDIVYIGLR 190 200 210 220 230 240 DVDPGEHYII KTLGIKYFSM TEVDKLGIGK VMEETFSYLL GRKKRPIHLS FDVDGLDPAF 250 260 270 280 290 300 TPATGTPVLG GLSYREGLYI TEEIYKTGLL SGLDIMEVNP TLGKTAEEVK STVNTAVALT 310 320 LACFGTQREG NHKPGTDYLK PPK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Chieko H. Submitted (MAR-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: C57BL/6 X CBA. Tissue: Liver.
[2]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Liver.
[3]	"Cloning of mouse full open reading frames in Gateway(R) system entry vector (pDONR201)." Ebert L., Muenstermann E., Schatten R., Henze S., Bohn E., Mollenhauer J., Wiemann S., Schick M., Korn B. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: FVB/N. Tissue: Liver and Mammary gland.
[5]	"Protein phosphorylation and expression profiling by Yin-yang multidimensional liquid chromatography (Yin-yang MDLC) mass spectrometry." Dai J., Jin W.-H., Sheng Q.-H., Shieh C.-H., Wu J.-R., Zeng R. J. Proteome Res. 6:250-262(2007) [PubMed: 17203969] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-230, MASS SPECTROMETRY. Tissue: Liver.
[6]	"Large-scale phosphorylation analysis of mouse liver." Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed: 17242355] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-3, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U51805 mRNA. Translation: AAA98611.1. AK149471 mRNA. Translation: BAE28901.1. Different initiation. AK171417 mRNA. Translation: BAE42440.1. CT010173 mRNA. Translation: CAJ18381.1. BC013341 mRNA. Translation: AAH13341.1. BC050005 mRNA. Translation: AAH50005.2.
IPI	IPI00117914.
RefSeq	NP_031508.1.
UniGene	Mm.154144
3D structure databases
HSSP	HSSP built from PDB template 1D3V based on UniProtKB P07824.
SMR	Q61176. Positions 6-319.
ModBase	Search...
Protein-protein interaction databases
STRING	Q61176.
PTM databases
PhosphoSite	Q61176.
2-D gel databases
SWISS-2DPAGE	Q61176.
Proteomic databases
PRIDE	Q61176.
Genome annotation databases
Ensembl	ENSMUST00000020161; ENSMUSP00000020161; ENSMUSG00000019987; Mus musculus. [Genome view]
GeneID	11846.
KEGG	mmu:11846.
NMPDR	fig|10090.3.peg.13476.
UCSC	uc007erg.1. mouse.
Organism-specific databases
CTD	11846.
MGI	MGI:88070. Arg1.
Phylogenomic databases
HOVERGEN	Q61176.
Enzyme and pathway databases
BRENDA	3.5.3.1. 244.
Gene expression databases
ArrayExpress	Q61176.
Bgee	Q61176.
CleanEx	MM_ARG1.
Genevestigator	Q61176.
GermOnline	ENSMUSG00000019987. Mus musculus.
Family and domain databases
InterPro	IPR005924. Arginase. IPR014033. Arginase_sub. IPR006035. Ureohydrolase. [Graphical view]
Gene3D	G3DSA:3.40.800.10. Ureohydrolase. 1 hit.
PANTHER	PTHR11358:SF2. Arginase_sub. 1 hit. PTHR11358. Ureohydrolase. 1 hit.
Pfam	PF00491. Arginase. 1 hit. [Graphical view]
PRINTS	PR00116. ARGINASE.
TIGRFAMs	TIGR01229. rocF_arginase. 1 hit.
PROSITE	PS01053. ARGINASE_1. 1 hit. PS51409. ARGINASE_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	279807.
SOURCE	Search...

Entry information

Entry name

ARGI1_MOUSE

Accession

Primary (citable) accession number: Q61176
Secondary accession number(s): Q3TB74 , Q3UEL0, Q4FK78, Q80VI4

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	October 13, 2009
This is version 77 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)