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Q61169 (GATA6_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 120. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Transcription factor GATA-6
Alternative name(s):
GATA-binding factor 6
Gene names
Name:Gata6
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length589 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Transcriptional activator that regulates SEMA3C and PLXNA2. Involved in gene regulation specifically in the gastric epithelium By similarity. Ref.8

Subunit structure

Interacts with LMCD1. Ref.7

Subcellular location

Nucleus Ref.7.

Tissue specificity

Expressed in myocardium, vascular smooth muscle, gut epithelium, and osteoclasts.

Domain

The GATA-type zinc fingers mediate interaction with LMCD1. Ref.7

Sequence similarities

Contains 2 GATA-type zinc fingers.

Sequence caution

The sequence AAB37426.1 differs from that shown. Reason: Frameshift at positions 360 and 368.

The sequence AAC52841.1 differs from that shown. Reason: Frameshift at position 572.

Ontologies

Keywords
   Biological processTranscription
Transcription regulation
   Cellular componentNucleus
   Coding sequence diversityAlternative initiation
   DomainRepeat
Zinc-finger
   LigandDNA-binding
Metal-binding
Zinc
   Molecular functionActivator
   PTMPhosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processClara cell differentiation

Inferred from genetic interaction PubMed 17164424. Source: MGI

Type II pneumocyte differentiation

Inferred from genetic interaction PubMed 17164424. Source: MGI

cardiac muscle cell differentiation

Inferred from expression pattern PubMed 16137232. Source: BHF-UCL

cardiac muscle hypertrophy in response to stress

Inferred from mutant phenotype PubMed 20705924. Source: MGI

cardiac muscle tissue development

Inferred from genetic interaction PubMed 20705924. Source: MGI

cardiac vascular smooth muscle cell differentiation

Inferred from electronic annotation. Source: Ensembl

cellular response to BMP stimulus

Inferred from direct assay PubMed 20501701. Source: MGI

cellular response to gonadotropin stimulus

Inferred from direct assay PubMed 9231805. Source: MGI

cellular response to hypoxia

Inferred from electronic annotation. Source: Ensembl

endodermal cell differentiation

Inferred from mutant phenotype PubMed 9832509. Source: MGI

endodermal cell fate determination

Inferred from direct assay PubMed 20308546. Source: MGI

epithelial cell differentiation

Inferred from mutant phenotype PubMed 16887115. Source: MGI

in utero embryonic development

Inferred from mutant phenotype PubMed 20123909PubMed 9832509. Source: MGI

intestinal epithelial cell differentiation

Inferred from sequence orthology PubMed 9566909. Source: MGI

liver development

Inferred from mutant phenotype PubMed 15767668. Source: MGI

lung saccule development

Inferred from genetic interaction PubMed 17164424. Source: MGI

male gonad development

Inferred from electronic annotation. Source: Ensembl

negative regulation of apoptotic process

Inferred from electronic annotation. Source: Ensembl

negative regulation of transcription from RNA polymerase II promoter

Inferred from electronic annotation. Source: Ensembl

negative regulation of transforming growth factor beta1 production

Inferred from electronic annotation. Source: Ensembl

negative regulation of transforming growth factor beta2 production

Inferred from electronic annotation. Source: Ensembl

organ formation

Inferred from mutant phenotype PubMed 16887115. Source: MGI

outflow tract septum morphogenesis

Inferred from electronic annotation. Source: Ensembl

pancreas development

Inferred from mutant phenotype PubMed 16887115. Source: MGI

pancreatic A cell differentiation

Inferred from mutant phenotype PubMed 16887115. Source: MGI

phospholipid metabolic process

Inferred from genetic interaction PubMed 17164424. Source: MGI

positive regulation of angiogenesis

Inferred from electronic annotation. Source: Ensembl

positive regulation of cardiac muscle cell proliferation

Inferred from genetic interaction PubMed 19084512. Source: MGI

positive regulation of cardioblast differentiation

Inferred from expression pattern PubMed 16137232. Source: BHF-UCL

positive regulation of cell cycle arrest

Inferred from electronic annotation. Source: Ensembl

positive regulation of transcription from RNA polymerase II promoter

Inferred from mutant phenotype PubMed 16137232. Source: BHF-UCL

positive regulation of transcription, DNA-templated

Inferred from direct assay PubMed 11733512Ref.1PubMed 9738004. Source: MGI

regulation of gene expression

Inferred from genetic interaction PubMed 18400219. Source: MGI

response to drug

Inferred from electronic annotation. Source: Ensembl

response to estrogen

Inferred from direct assay PubMed 9231805. Source: MGI

tube morphogenesis

Inferred from genetic interaction PubMed 18400219. Source: MGI

type B pancreatic cell differentiation

Inferred from mutant phenotype PubMed 16887115. Source: MGI

   Cellular_componentnucleus

Inferred from direct assay PubMed 12130579Ref.7PubMed 16940177PubMed 20123909PubMed 20705924. Source: MGI

transcription factor complex

Inferred from direct assay PubMed 17164424. Source: MGI

   Molecular_functionDNA binding

Inferred from direct assay PubMed 11733512PubMed 15987774Ref.7PubMed 16621466PubMed 16912278. Source: MGI

RNA polymerase II core promoter sequence-specific DNA binding

Inferred from direct assay PubMed 21350014. Source: MGI

RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity

Inferred from direct assay Ref.1. Source: MGI

RNA polymerase II repressing transcription factor binding

Inferred from physical interaction PubMed 16293227. Source: BHF-UCL

chromatin binding

Inferred from direct assay Ref.7. Source: MGI

sequence-specific DNA binding

Inferred from direct assay PubMed 11914369. Source: MGI

sequence-specific DNA binding transcription factor activity

Inferred from direct assay PubMed 11914369PubMed 16621466PubMed 17164424. Source: MGI

transcription factor binding

Inferred from physical interaction PubMed 16887115. Source: MGI

zinc ion binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative initiation. [Align] [Select]
Isoform 1 (identifier: Q61169-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q61169-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-146: Missing.
Note: Produced by alternative initiation at Met-147 of isoform 1.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 589589Transcription factor GATA-6
PRO_0000083424

Regions

Zinc finger384 – 40825GATA-type 1
Zinc finger438 – 46225GATA-type 2
Compositional bias173 – 18311Poly-Ala
Compositional bias322 – 3287Poly-His
Compositional bias443 – 4475Poly-Thr

Amino acid modifications

Modified residue2661Phosphoserine By similarity

Natural variations

Alternative sequence1 – 146146Missing in isoform 2.
VSP_035779

Experimental info

Sequence conflict2971S → T in AAB37426. Ref.1
Sequence conflict4161G → A in AAC52841. Ref.5
Sequence conflict5361G → R in AAC52841. Ref.5
Sequence conflict5381Missing in AAC52841. Ref.5
Sequence conflict5461N → K in AAC52841. Ref.5
Sequence conflict5891A → V in AAB37426. Ref.1
Sequence conflict5891A → V in AAD55267. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified November 25, 2008. Version 3.
Checksum: 1A55474DA65EC6ED

FASTA58959,307
        10         20         30         40         50         60 
MALTDGGWCL PKRFGAAAAD AGDSGPFPAR EPSSPLSPIS SSSSSCSRGG DRGPCGASNC 

        70         80         90        100        110        120 
RTPQLDAEAV AGPPGRSLLL SPYASHPFAA AHGAAAPGVA GPGSALSTWE DLLLFTDLDQ 

       130        140        150        160        170        180 
AATASKLLWS SRGAKLSPFA AEQPEEMYQT LAALSSQGPA AYDGAPGGFV HSAAAAAAAA 

       190        200        210        220        230        240 
AAASSPVYVP TTRVGSMLSG LPYLQGAGSG PSNHAGGAGA HPGWSQASAD SPPYGGGGAA 

       250        260        270        280        290        300 
GGGAAGPGGA GSATAHASAR FPYSPSPPMA NGAARDPGGY VAAGGTGAGS VSGGGGSLAA 

       310        320        330        340        350        360 
MGGREHQYSS LSAARPLNGT YHHHHHHHPT YSPYMAAPLT PAWPAGPFET PVLHSLQGRA 

       370        380        390        400        410        420 
GAPLPVPRGP STDLLEDLSE SRECVNCGSI QTPLWRRDGT GHYLCNACGL YSKMNGLSRP 

       430        440        450        460        470        480 
LIKPQKRVPS SRRLGLSCAN CHTTTTTLWR RNAEGEPVCN ACGLYMKLHG VPRPLAMKKE 

       490        500        510        520        530        540 
GIQTRKRKPK NINKSKACSG NSSGSVPMTP TSSSSNSDDC TKNTSPSTQA TTSGVGASVM 

       550        560        570        580 
SAVGENANPE NSDLKYSGQD GLYIGVSLSS PAEVTSSVRQ DSWCALALA 

« Hide

Isoform 2 [UniParc].

Checksum: 27B98CD21D42D223
Show »

FASTA44344,702

References

« Hide 'large scale' references
[1]"GATA-6: a zinc finger transcription factor that is expressed in multiple cell lineages derived from lateral mesoderm."
Morrisey E.E., Ip H.S., Lu M.M., Parmacek M.S.
Dev. Biol. 177:309-322(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
Tissue: Heart.
[2]Katsuoka F., Motohashi H., Yamamoto M.
Submitted (AUG-1999) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
Strain: C57BL/6 X DBA/2.
Tissue: Heart.
[3]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Strain: C57BL/6J.
Tissue: Lung.
[4]Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]"The gene for transcription factor GATA-6 resides on mouse chromosome 18 and is expressed in myocardium and vascular smooth muscle."
Narita N., Heikinheimo M., Bielinska M., White R.A., Wilson D.B.
Genomics 36:345-348(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 384-589 (ISOFORM 1).
Strain: BALB/c.
Tissue: Heart.
[6]"The human and mouse GATA-6 genes utilize two promoters and two initiation codons."
Brewer A., Gove C., Davies A., McNulty C., Barrow D., Koutsourakis M., Farzaneh F., Pizzey J., Bomford A., Patient R.
J. Biol. Chem. 274:38004-38016(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: ALTERNATIVE INITIATION.
[7]"LMCD1/Dyxin is a novel transcriptional cofactor that restricts GATA6 function by inhibiting DNA binding."
Rath N., Wang Z., Lu M.M., Morrisey E.E.
Mol. Cell. Biol. 25:8864-8873(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION, INTERACTION WITH LMCD1, DOMAIN GATA-TYPE ZINC-FINGER.
[8]"GATA6 mutations cause human cardiac outflow tract defects by disrupting semaphorin-plexin signaling."
Kodo K., Nishizawa T., Furutani M., Arai S., Yamamura E., Joo K., Takahashi T., Matsuoka R., Yamagishi H.
Proc. Natl. Acad. Sci. U.S.A. 106:13933-13938(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
S82462 mRNA. Translation: AAB37426.1. Frameshift.
AF179425 mRNA. Translation: AAD55267.1.
AK142381 mRNA. Translation: BAE25049.1.
CH466592 Genomic DNA. Translation: EDL22985.1.
U51335 mRNA. Translation: AAC52841.1. Frameshift.
RefSeqNP_034388.2. NM_010258.3.
UniGeneMm.329287.

3D structure databases

ProteinModelPortalQ61169.
SMRQ61169. Positions 380-487.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid199843. 12 interactions.
IntActQ61169. 1 interaction.
MINTMINT-1782188.
STRING10090.ENSMUSP00000041774.

PTM databases

PhosphoSiteQ61169.

Proteomic databases

PRIDEQ61169.

Protocols and materials databases

DNASU14465.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000047762; ENSMUSP00000041774; ENSMUSG00000005836. [Q61169-1]
GeneID14465.
KEGGmmu:14465.
UCSCuc008ebj.1. mouse. [Q61169-1]

Organism-specific databases

CTD2627.
MGIMGI:107516. Gata6.

Phylogenomic databases

eggNOGCOG5641.
GeneTreeENSGT00750000117615.
HOGENOMHOG000047700.
HOVERGENHBG051703.
InParanoidQ3UQJ2.
KOK17897.
OMAGAHPGWP.
OrthoDBEOG7CCBRF.
PhylomeDBQ61169.
TreeFamTF315391.

Gene expression databases

ArrayExpressQ61169.
BgeeQ61169.
CleanExMM_GATA6.
GenevestigatorQ61169.

Family and domain databases

Gene3D3.30.50.10. 2 hits.
InterProIPR008013. GATA_N.
IPR028437. TF_GATA_6.
IPR000679. Znf_GATA.
IPR013088. Znf_NHR/GATA.
[Graphical view]
PANTHERPTHR10071:SF23. PTHR10071:SF23. 1 hit.
PfamPF00320. GATA. 2 hits.
PF05349. GATA-N. 1 hit.
[Graphical view]
PRINTSPR00619. GATAZNFINGER.
SMARTSM00401. ZnF_GATA. 2 hits.
[Graphical view]
PROSITEPS00344. GATA_ZN_FINGER_1. 2 hits.
PS50114. GATA_ZN_FINGER_2. 2 hits.
[Graphical view]
ProtoNetSearch...

Other

NextBio286112.
PROQ61169.
SOURCESearch...

Entry information

Entry nameGATA6_MOUSE
AccessionPrimary (citable) accession number: Q61169
Secondary accession number(s): P97729, Q3UQJ2, Q9QZK3
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 25, 2008
Last modified: April 16, 2014
This is version 120 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot