Q61169 (GATA6_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Transcription factor GATA-6

Alternative name(s):
GATA-binding factor 6

Gene names

Name:

Gata6

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	589 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Transcriptional activator that regulates SEMA3C and PLXNA2. Involved in gene regulation specifically in the gastric epithelium By similarity. Ref.8
Subunit structure	Interacts with LMCD1. Ref.7
Subcellular location	Nucleus Ref.7.
Tissue specificity	Expressed in myocardium, vascular smooth muscle, gut epithelium, and osteoclasts.
Domain	The GATA-type zinc fingers mediate interaction with LMCD1. Ref.7
Sequence similarities	Contains 2 GATA-type zinc fingers.
Sequence caution	The sequence AAB37426.1 differs from that shown. Reason: Frameshift at positions 360 and 368. The sequence AAC52841.1 differs from that shown. Reason: Frameshift at position 572.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Coding sequence diversity	Alternative initiation
Domain	Repeat Zinc-finger
Ligand	DNA-binding Metal-binding Zinc
Molecular function	Activator
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	Clara cell differentiation Inferred from genetic interaction PubMed 17164424. Source: MGI Type II pneumocyte differentiation Inferred from genetic interaction PubMed 17164424. Source: MGI apoptotic process Inferred from mutant phenotype PubMed 9832509. Source: MGI cardiac muscle cell differentiation Inferred from expression pattern PubMed 16137232. Source: BHF-UCL cardiac vascular smooth muscle cell differentiation Inferred from electronic annotation. Source: Compara cellular response to BMP stimulus Inferred from direct assay PubMed 20501701. Source: MGI cellular response to gonadotropin stimulus Inferred from direct assay PubMed 9231805. Source: MGI cellular response to hypoxia Inferred from electronic annotation. Source: Compara endodermal cell fate determination Inferred from direct assay PubMed 20308546. Source: MGI in utero embryonic development Inferred from mutant phenotype PubMed 20123909PubMed 9832509. Source: MGI intestinal epithelial cell differentiation Inferred from sequence orthology PubMed 9566909. Source: MGI liver development Inferred from mutant phenotype PubMed 15767668. Source: MGI lung saccule development Inferred from genetic interaction PubMed 17164424. Source: MGI male gonad development Inferred from electronic annotation. Source: Compara negative regulation of apoptotic process Inferred from electronic annotation. Source: Compara negative regulation of transcription from RNA polymerase II promoter Inferred from electronic annotation. Source: Compara negative regulation of transforming growth factor beta1 production Inferred from electronic annotation. Source: Compara negative regulation of transforming growth factor beta2 production Inferred from electronic annotation. Source: Compara organ formation Inferred from mutant phenotype PubMed 16887115. Source: MGI outflow tract septum morphogenesis Inferred from electronic annotation. Source: Compara pancreatic A cell differentiation Inferred from mutant phenotype PubMed 16887115. Source: MGI phospholipid metabolic process Inferred from genetic interaction PubMed 17164424. Source: MGI positive regulation of angiogenesis Inferred from electronic annotation. Source: Compara positive regulation of cardiac muscle cell proliferation Inferred from genetic interaction PubMed 19084512. Source: MGI positive regulation of cardioblast differentiation Inferred from expression pattern PubMed 16137232. Source: BHF-UCL positive regulation of cell cycle arrest Inferred from electronic annotation. Source: Compara positive regulation of transcription from RNA polymerase II promoter Inferred from mutant phenotype PubMed 16137232. Source: BHF-UCL response to drug Inferred from electronic annotation. Source: Compara response to estrogen stimulus Inferred from direct assay PubMed 9231805. Source: MGI tube morphogenesis Inferred from genetic interaction PubMed 18400219. Source: MGI type B pancreatic cell differentiation Inferred from mutant phenotype PubMed 16887115. Source: MGI
Cellular_component	transcription factor complex Inferred from direct assay PubMed 17164424. Source: MGI
Molecular_function	RNA polymerase II core promoter sequence-specific DNA binding Inferred from direct assay PubMed 21350014. Source: MGI RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity Inferred from direct assay Ref.1. Source: MGI chromatin binding Inferred from direct assay Ref.7. Source: MGI zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative initiation. [Align] [Select]

Isoform 1 (identifier: Q61169-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q61169-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-146: Missing.

Note: Produced by alternative initiation at Met-147 of isoform 1.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 589	589	Transcription factor GATA-6		PRO_0000083424
Regions
	Zinc finger	384 – 408	25	GATA-type 1
	Zinc finger	438 – 462	25	GATA-type 2
	Compositional bias	173 – 183	11	Poly-Ala
	Compositional bias	322 – 328	7	Poly-His
	Compositional bias	443 – 447	5	Poly-Thr
Amino acid modifications
	Modified residue	266	1	Phosphoserine By similarity
Natural variations
	Alternative sequence	1 – 146	146	Missing in isoform 2.		VSP_035779
Experimental info
	Sequence conflict	297	1	S → T in AAB37426. Ref.1
	Sequence conflict	416	1	G → A in AAC52841. Ref.5
	Sequence conflict	536	1	G → R in AAC52841. Ref.5
	Sequence conflict	538	1	Missing in AAC52841. Ref.5
	Sequence conflict	546	1	N → K in AAC52841. Ref.5
	Sequence conflict	589	1	A → V in AAB37426. Ref.1
	Sequence conflict	589	1	A → V in AAD55267. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified November 25, 2008. Version 3. Checksum: 1A55474DA65EC6ED	FASTA	589	59,307
	10 20 30 40 50 60 MALTDGGWCL PKRFGAAAAD AGDSGPFPAR EPSSPLSPIS SSSSSCSRGG DRGPCGASNC 70 80 90 100 110 120 RTPQLDAEAV AGPPGRSLLL SPYASHPFAA AHGAAAPGVA GPGSALSTWE DLLLFTDLDQ 130 140 150 160 170 180 AATASKLLWS SRGAKLSPFA AEQPEEMYQT LAALSSQGPA AYDGAPGGFV HSAAAAAAAA 190 200 210 220 230 240 AAASSPVYVP TTRVGSMLSG LPYLQGAGSG PSNHAGGAGA HPGWSQASAD SPPYGGGGAA 250 260 270 280 290 300 GGGAAGPGGA GSATAHASAR FPYSPSPPMA NGAARDPGGY VAAGGTGAGS VSGGGGSLAA 310 320 330 340 350 360 MGGREHQYSS LSAARPLNGT YHHHHHHHPT YSPYMAAPLT PAWPAGPFET PVLHSLQGRA 370 380 390 400 410 420 GAPLPVPRGP STDLLEDLSE SRECVNCGSI QTPLWRRDGT GHYLCNACGL YSKMNGLSRP 430 440 450 460 470 480 LIKPQKRVPS SRRLGLSCAN CHTTTTTLWR RNAEGEPVCN ACGLYMKLHG VPRPLAMKKE 490 500 510 520 530 540 GIQTRKRKPK NINKSKACSG NSSGSVPMTP TSSSSNSDDC TKNTSPSTQA TTSGVGASVM 550 560 570 580 SAVGENANPE NSDLKYSGQD GLYIGVSLSS PAEVTSSVRQ DSWCALALA
	Isoform 2 [UniParc]. Checksum: 27B98CD21D42D223	FASTA	443	44,702
	10 20 30 40 50 60 MYQTLAALSS QGPAAYDGAP GGFVHSAAAA AAAAAAASSP VYVPTTRVGS MLSGLPYLQG 70 80 90 100 110 120 AGSGPSNHAG GAGAHPGWSQ ASADSPPYGG GGAAGGGAAG PGGAGSATAH ASARFPYSPS 130 140 150 160 170 180 PPMANGAARD PGGYVAAGGT GAGSVSGGGG SLAAMGGREH QYSSLSAARP LNGTYHHHHH 190 200 210 220 230 240 HHPTYSPYMA APLTPAWPAG PFETPVLHSL QGRAGAPLPV PRGPSTDLLE DLSESRECVN 250 260 270 280 290 300 CGSIQTPLWR RDGTGHYLCN ACGLYSKMNG LSRPLIKPQK RVPSSRRLGL SCANCHTTTT 310 320 330 340 350 360 TLWRRNAEGE PVCNACGLYM KLHGVPRPLA MKKEGIQTRK RKPKNINKSK ACSGNSSGSV 370 380 390 400 410 420 PMTPTSSSSN SDDCTKNTSP STQATTSGVG ASVMSAVGEN ANPENSDLKY SGQDGLYIGV 430 440 SLSSPAEVTS SVRQDSWCAL ALA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"GATA-6: a zinc finger transcription factor that is expressed in multiple cell lineages derived from lateral mesoderm." Morrisey E.E., Ip H.S., Lu M.M., Parmacek M.S. Dev. Biol. 177:309-322(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2). Tissue: Heart.
[2]	Katsuoka F., Motohashi H., Yamamoto M. Submitted (AUG-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2). Strain: C57BL/6 X DBA/2. Tissue: Heart.
[3]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: C57BL/6J. Tissue: Lung.
[4]	Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The gene for transcription factor GATA-6 resides on mouse chromosome 18 and is expressed in myocardium and vascular smooth muscle." Narita N., Heikinheimo M., Bielinska M., White R.A., Wilson D.B. Genomics 36:345-348(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 384-589 (ISOFORM 1). Strain: BALB/c. Tissue: Heart.
[6]	"The human and mouse GATA-6 genes utilize two promoters and two initiation codons." Brewer A., Gove C., Davies A., McNulty C., Barrow D., Koutsourakis M., Farzaneh F., Pizzey J., Bomford A., Patient R. J. Biol. Chem. 274:38004-38016(1999) [PubMed] [Europe PMC] [Abstract] Cited for: ALTERNATIVE INITIATION.
[7]	"LMCD1/Dyxin is a novel transcriptional cofactor that restricts GATA6 function by inhibiting DNA binding." Rath N., Wang Z., Lu M.M., Morrisey E.E. Mol. Cell. Biol. 25:8864-8873(2005) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION, INTERACTION WITH LMCD1, DOMAIN GATA-TYPE ZINC FINGER.
[8]	"GATA6 mutations cause human cardiac outflow tract defects by disrupting semaphorin-plexin signaling." Kodo K., Nishizawa T., Furutani M., Arai S., Yamamura E., Joo K., Takahashi T., Matsuoka R., Yamagishi H. Proc. Natl. Acad. Sci. U.S.A. 106:13933-13938(2009) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	S82462 mRNA. Translation: AAB37426.1. Frameshift. AF179425 mRNA. Translation: AAD55267.1. AK142381 mRNA. Translation: BAE25049.1. CH466592 Genomic DNA. Translation: EDL22985.1. U51335 mRNA. Translation: AAC52841.1. Frameshift.
IPI	IPI00411001. IPI00915073.
RefSeq	NP_034388.2. NM_010258.3.
UniGene	Mm.329287.
3D structure databases
ProteinModelPortal	Q61169.
SMR	Q61169. Positions 380-487.
ModBase	Search...
Protein-protein interaction databases
MINT	MINT-1782188.
STRING	10090.ENSMUSP00000041774.
PTM databases
PhosphoSite	Q61169.
Proteomic databases
PRIDE	Q61169.
Protocols and materials databases
DNASU	14465.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000047762; ENSMUSP00000041774; ENSMUSG00000005836.
GeneID	14465.
KEGG	mmu:14465.
UCSC	uc008ebk.1. mouse.
Organism-specific databases
CTD	2627.
MGI	MGI:107516. Gata6.
Phylogenomic databases
eggNOG	COG5641.
GeneTree	ENSGT00550000074470.
HOGENOM	HOG000047700.
HOVERGEN	HBG051703.
InParanoid	Q3UQJ2.
KO	K09183.
OMA	GAHPGWP.
OrthoDB	EOG4TMR21.
Gene expression databases
ArrayExpress	Q61169.
Bgee	Q61169.
CleanEx	MM_GATA6.
Genevestigator	Q61169.
GermOnline	ENSMUSG00000005836. Mus musculus.
Family and domain databases
Gene3D	3.30.50.10. 2 hits.
InterPro	IPR008013. GATA_N. IPR000679. Znf_GATA. IPR013088. Znf_NHR/GATA. [Graphical view]
Pfam	PF00320. GATA. 2 hits. PF05349. GATA-N. 1 hit. [Graphical view]
PRINTS	PR00619. GATAZNFINGER.
SMART	SM00401. ZnF_GATA. 2 hits. [Graphical view]
PROSITE	PS00344. GATA_ZN_FINGER_1. 2 hits. PS50114. GATA_ZN_FINGER_2. 2 hits. [Graphical view]
ProtoNet	Search...
Other
NextBio	286112.
SOURCE	Search...

Entry information

Entry name

GATA6_MOUSE

Accession

Primary (citable) accession number: Q61169
Secondary accession number(s): P97729, Q3UQJ2, Q9QZK3

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 25, 2008
Last modified:	May 1, 2013
This is version 111 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program