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Q61165

- SL9A1_MOUSE

UniProt

Q61165 - SL9A1_MOUSE

Protein

Sodium/hydrogen exchanger 1

Gene

Slc9a1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 128 (01 Oct 2014)
      Sequence version 1 (01 Nov 1997)
      Previous versions | rss
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    Functioni

    Involved in pH regulation to eliminate acids generated by active metabolism or to counter adverse environmental conditions. Major proton extruding system driven by the inward sodium ion chemical gradient. Plays an important role in signal transduction.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei165 – 1651Channel pore-liningBy similarity

    GO - Molecular functioni

    1. sodium:proton antiporter activity Source: MGI

    GO - Biological processi

    1. cardiac muscle cell differentiation Source: MGI
    2. cell growth Source: Ensembl
    3. cellular response to acidic pH Source: UniProtKB
    4. negative regulation of apoptotic process Source: Ensembl
    5. neuron death Source: Ensembl
    6. positive regulation of action potential Source: Ensembl
    7. positive regulation of cell growth Source: Ensembl
    8. positive regulation of mitochondrial membrane permeability Source: Ensembl
    9. protein oligomerization Source: UniProtKB
    10. regulation of intracellular pH Source: Ensembl
    11. regulation of pH Source: MGI
    12. regulation of sensory perception of pain Source: Ensembl
    13. regulation of the force of heart contraction Source: Ensembl
    14. response to acidic pH Source: MGI
    15. response to drug Source: Ensembl
    16. response to organic cyclic compound Source: Ensembl
    17. sodium ion export Source: UniProtKB
    18. sodium ion transport Source: MGI

    Keywords - Biological processi

    Antiport, Ion transport, Sodium transport, Transport

    Keywords - Ligandi

    Calmodulin-binding, Sodium

    Enzyme and pathway databases

    ReactomeiREACT_196554. Hyaluronan uptake and degradation.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Sodium/hydrogen exchanger 1
    Alternative name(s):
    Na(+)/H(+) exchanger 1
    Short name:
    NHE-1
    Solute carrier family 9 member 1
    Gene namesi
    Name:Slc9a1
    Synonyms:Nhe1
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 4

    Organism-specific databases

    MGIiMGI:102462. Slc9a1.

    Subcellular locationi

    Membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane By similarity; Multi-pass membrane protein By similarity. Cell membrane; Multi-pass membrane protein
    Note: Colocalizes with CHP1 and CHP2 at the reticulum endoplasmic and plasma membrane.By similarity

    GO - Cellular componenti

    1. apical plasma membrane Source: MGI
    2. basolateral plasma membrane Source: MGI
    3. cytoplasm Source: UniProtKB
    4. endoplasmic reticulum Source: UniProtKB
    5. endoplasmic reticulum membrane Source: UniProtKB-SubCell
    6. integral component of membrane Source: UniProtKB-KW
    7. membrane Source: MGI
    8. membrane raft Source: Ensembl
    9. mitochondrion Source: Ensembl
    10. plasma membrane Source: UniProtKB

    Keywords - Cellular componenti

    Cell membrane, Endoplasmic reticulum, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 820820Sodium/hydrogen exchanger 1PRO_0000052348Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi374 – 3741N-linked (GlcNAc...)Sequence Analysis
    Modified residuei603 – 6031Phosphoserine1 Publication
    Modified residuei609 – 6091Phosphoserine1 Publication
    Modified residuei697 – 6971Phosphoserine2 Publications
    Modified residuei701 – 7011PhosphoserineBy similarity
    Modified residuei707 – 7071Phosphoserine2 Publications
    Modified residuei727 – 7271PhosphoserineBy similarity
    Modified residuei730 – 7301PhosphoserineBy similarity
    Modified residuei733 – 7331PhosphoserineBy similarity
    Modified residuei790 – 7901PhosphoserineBy similarity

    Post-translational modificationi

    O-glycosylated.By similarity
    Ubiquitinated, leading to its degradation by the proteasome. Ubiquitination is reduced by CHP1 By similarity.By similarity

    Keywords - PTMi

    Glycoprotein, Phosphoprotein, Ubl conjugation

    Proteomic databases

    MaxQBiQ61165.
    PaxDbiQ61165.
    PRIDEiQ61165.

    PTM databases

    PhosphoSiteiQ61165.

    Expressioni

    Gene expression databases

    ArrayExpressiQ61165.
    BgeeiQ61165.
    GenevestigatoriQ61165.

    Interactioni

    Subunit structurei

    Oligomer. Interacts with calmodulin and TESC. Interacts (via the juxtamembrane region of the cytoplasmic C-terminal domain) with CHP1; the interaction occurs at the plasma membrane in a calcium-dependent manner. Interacts with CHP2; the interaction occurs in a calcium-dependent manner By similarity.By similarity

    Protein-protein interaction databases

    BioGridi203321. 3 interactions.
    IntActiQ61165. 1 interaction.

    Structurei

    3D structure databases

    ProteinModelPortaliQ61165.
    SMRiQ61165. Positions 159-184, 342-369, 451-476, 507-549, 626-689.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 1212CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini34 – 10572ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini127 – 1337CytoplasmicSequence Analysis
    Topological domaini154 – 1585ExtracellularSequence Analysis
    Topological domaini179 – 19517CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini216 – 23116ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini252 – 2609CytoplasmicSequence Analysis
    Topological domaini281 – 29818ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini320 – 34223CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini363 – 38523ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini407 – 4148ExtracellularSequence Analysis
    Topological domaini435 – 45218CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini475 – 4839ExtracellularSequence Analysis
    Topological domaini504 – 820317CytoplasmicSequence AnalysisAdd
    BLAST

    Intramembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Intramembranei386 – 40621Name=H10By similarityAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei13 – 3321Helical; Name=M1Sequence AnalysisAdd
    BLAST
    Transmembranei106 – 12621Helical; Name=M2Sequence AnalysisAdd
    BLAST
    Transmembranei134 – 15320Helical; Name=M3Sequence AnalysisAdd
    BLAST
    Transmembranei159 – 17820Helical; Name=M4Sequence AnalysisAdd
    BLAST
    Transmembranei196 – 21520Helical; Name=M5Sequence AnalysisAdd
    BLAST
    Transmembranei232 – 25120Helical; Name=M6Sequence AnalysisAdd
    BLAST
    Transmembranei261 – 28020Helical; Name=M7Sequence AnalysisAdd
    BLAST
    Transmembranei299 – 31921Helical; Name=M8Sequence AnalysisAdd
    BLAST
    Transmembranei343 – 36220Helical; Name=M9Sequence AnalysisAdd
    BLAST
    Transmembranei415 – 43420Helical; Name=M10Sequence AnalysisAdd
    BLAST
    Transmembranei453 – 47422Helical; Name=M11Sequence AnalysisAdd
    BLAST
    Transmembranei484 – 50320Helical; Name=M12Sequence AnalysisAdd
    BLAST

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni520 – 54324Interaction with CHP2By similarityAdd
    BLAST

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG0025.
    GeneTreeiENSGT00560000077158.
    HOGENOMiHOG000247044.
    HOVERGENiHBG052615.
    KOiK05742.
    OMAiYDRVGIV.
    OrthoDBiEOG7XWPN4.
    TreeFamiTF317212.

    Family and domain databases

    InterProiIPR006153. Cation/H_exchanger.
    IPR018422. Cation/H_exchanger_CPA1.
    IPR001970. Na/H_exchanger_1.
    IPR004709. NaH_exchanger.
    [Graphical view]
    PANTHERiPTHR10110. PTHR10110. 1 hit.
    PfamiPF00999. Na_H_Exchanger. 1 hit.
    [Graphical view]
    PRINTSiPR01084. NAHEXCHNGR.
    PR01085. NAHEXCHNGR1.
    TIGRFAMsiTIGR00840. b_cpa1. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q61165-1 [UniParc]FASTAAdd to Basket

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    MMLRWSGVWG FHPPRIFPSL LVVVALVGLL PVLRSHGLQH SPTASTIRGS    50
    EPPRERSIGD VTTAPSEPLH RPDDHNLTNL IIEHGGKPSR KAFPVLDIDY 100
    PHVRTPFEIS LWILLACLMK IGFHVIPTIS SIVPESCLLI VVGLLVGGLI 150
    KGVGETPPFL QSDVFFLFLL PPIILDAGYF LPLRQFTENL GTILIFAVVG 200
    TLWNAFFLGG LLYAVCLVGG EQINNIGLLD TLLFGSIISA VDPVAVLAVF 250
    EEIHINELLH ILVFGESLLN DAVTVVLYHL FEEFASYDSV GISDIFLGFL 300
    SFFVVALGGV FVGVVYGVIA AFTSRFTSHI RVIEPLFVFL YSYMAYLSAE 350
    LFHLSGIMAL IASGVVMRPY VEANISHKSH TTIKYFLKMW SSVSETLIFI 400
    FLGVSTVAGS HQWNWTFVIS TLLFCLIARV LGVLVLTWFI NKFRIVKLTP 450
    KDQFIIAYGG LRGAIAFSLG YLLDKKHFPM CDLFLTAIIT VIFFTVFVQG 500
    MTIRPLVDLL AVKKKQETKR SINEEIHTQF LDHLLTGIED ICGHYGHHHW 550
    KDKLNRFNKK YVKKCLIAGE RSKEPQLIAF YHKMEMKQAI ELVESGGMGK 600
    IPSAVSTVSM QNIHPKAVTS DRILPALSKD KEEEIRKILR SNLQKTRQRL 650
    RSYNRHTLVA DPYEEAWNQM LLRRQKARQL EQKITNYLTV PAHKLDSPTL 700
    SRARIGSDPL AYEPKADLPV ITIDPASPQS PESVDLVNEE LKGKVLGLNR 750
    GPRVTPEEEE EDEDGIIMIR SKEPSSPGTD DVFTPGSSDS PSSQRIQRCL 800
    SDPGPHPEPG EGEPFIPKGQ 820
    Length:820
    Mass (Da):91,468
    Last modified:November 1, 1997 - v1
    Checksum:i0589C4D08DD348BE
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U51112 mRNA. Translation: AAA92976.1.
    CCDSiCCDS38903.1.
    RefSeqiNP_058677.1. NM_016981.2.
    UniGeneiMm.4312.

    Genome annotation databases

    EnsembliENSMUST00000030669; ENSMUSP00000030669; ENSMUSG00000028854.
    GeneIDi20544.
    KEGGimmu:20544.
    UCSCiuc008vcs.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U51112 mRNA. Translation: AAA92976.1 .
    CCDSi CCDS38903.1.
    RefSeqi NP_058677.1. NM_016981.2.
    UniGenei Mm.4312.

    3D structure databases

    ProteinModelPortali Q61165.
    SMRi Q61165. Positions 159-184, 342-369, 451-476, 507-549, 626-689.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 203321. 3 interactions.
    IntActi Q61165. 1 interaction.

    PTM databases

    PhosphoSitei Q61165.

    Proteomic databases

    MaxQBi Q61165.
    PaxDbi Q61165.
    PRIDEi Q61165.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000030669 ; ENSMUSP00000030669 ; ENSMUSG00000028854 .
    GeneIDi 20544.
    KEGGi mmu:20544.
    UCSCi uc008vcs.1. mouse.

    Organism-specific databases

    CTDi 6548.
    MGIi MGI:102462. Slc9a1.

    Phylogenomic databases

    eggNOGi COG0025.
    GeneTreei ENSGT00560000077158.
    HOGENOMi HOG000247044.
    HOVERGENi HBG052615.
    KOi K05742.
    OMAi YDRVGIV.
    OrthoDBi EOG7XWPN4.
    TreeFami TF317212.

    Enzyme and pathway databases

    Reactomei REACT_196554. Hyaluronan uptake and degradation.

    Miscellaneous databases

    NextBioi 298831.
    PROi Q61165.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q61165.
    Bgeei Q61165.
    Genevestigatori Q61165.

    Family and domain databases

    InterProi IPR006153. Cation/H_exchanger.
    IPR018422. Cation/H_exchanger_CPA1.
    IPR001970. Na/H_exchanger_1.
    IPR004709. NaH_exchanger.
    [Graphical view ]
    PANTHERi PTHR10110. PTHR10110. 1 hit.
    Pfami PF00999. Na_H_Exchanger. 1 hit.
    [Graphical view ]
    PRINTSi PR01084. NAHEXCHNGR.
    PR01085. NAHEXCHNGR1.
    TIGRFAMsi TIGR00840. b_cpa1. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "cDNA cloning and expression of the mouse Na/H antiporter (NHE-1) and a potential splice variant."
      Dewey M.J., Ennis T.M., Bowman L.H.
      Mol. Biol. Rep. 28:111-117(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: BALB/c.
    2. "Qualitative and quantitative analyses of protein phosphorylation in naive and stimulated mouse synaptosomal preparations."
      Munton R.P., Tweedie-Cullen R., Livingstone-Zatchej M., Weinandy F., Waidelich M., Longo D., Gehrig P., Potthast F., Rutishauser D., Gerrits B., Panse C., Schlapbach R., Mansuy I.M.
      Mol. Cell. Proteomics 6:283-293(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Brain cortex.
    3. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-603; SER-609 AND SER-707, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Liver.
    4. "The phagosomal proteome in interferon-gamma-activated macrophages."
      Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
      Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-697 AND SER-707, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    5. "Large scale localization of protein phosphorylation by use of electron capture dissociation mass spectrometry."
      Sweet S.M., Bailey C.M., Cunningham D.L., Heath J.K., Cooper H.J.
      Mol. Cell. Proteomics 8:904-912(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-697, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Embryonic fibroblast.

    Entry informationi

    Entry nameiSL9A1_MOUSE
    AccessioniPrimary (citable) accession number: Q61165
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: November 1, 1997
    Last modified: October 1, 2014
    This is version 128 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Caution

    The region between transmembrane regions M4 and M5 and between M6 and M7 (also termed intracellular loops IL2 and IL4, respectively) seem to be localized at least in part in the membrane. The hydrophobic region H10 is proposed to be located within the membrane.Curated

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3