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Protein

Sodium/hydrogen exchanger 1

Gene

Slc9a1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in pH regulation to eliminate acids generated by active metabolism or to counter adverse environmental conditions. Major proton extruding system driven by the inward sodium ion chemical gradient. Plays an important role in signal transduction.

Caution

The region between transmembrane regions M4 and M5 and between M6 and M7 (also termed intracellular loops IL2 and IL4, respectively) seem to be localized at least in part in the membrane. The hydrophobic region H10 is proposed to be located within the membrane.Curated

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei165Channel pore-liningBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionCalmodulin-binding
Biological processAntiport, Ion transport, Sodium transport, Transport
LigandSodium

Enzyme and pathway databases

ReactomeiR-MMU-2160916 Hyaluronan uptake and degradation
R-MMU-425986 Sodium/Proton exchangers

Names & Taxonomyi

Protein namesi
Recommended name:
Sodium/hydrogen exchanger 1
Alternative name(s):
Na(+)/H(+) exchanger 1
Short name:
NHE-1
Solute carrier family 9 member 1
Gene namesi
Name:Slc9a1
Synonyms:Nhe1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi

Organism-specific databases

MGIiMGI:102462 Slc9a1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 12CytoplasmicSequence analysisAdd BLAST12
Transmembranei13 – 33Helical; Name=M1Sequence analysisAdd BLAST21
Topological domaini34 – 105ExtracellularSequence analysisAdd BLAST72
Transmembranei106 – 126Helical; Name=M2Sequence analysisAdd BLAST21
Topological domaini127 – 133CytoplasmicSequence analysis7
Transmembranei134 – 153Helical; Name=M3Sequence analysisAdd BLAST20
Topological domaini154 – 158ExtracellularSequence analysis5
Transmembranei159 – 178Helical; Name=M4Sequence analysisAdd BLAST20
Topological domaini179 – 195CytoplasmicSequence analysisAdd BLAST17
Transmembranei196 – 215Helical; Name=M5Sequence analysisAdd BLAST20
Topological domaini216 – 231ExtracellularSequence analysisAdd BLAST16
Transmembranei232 – 251Helical; Name=M6Sequence analysisAdd BLAST20
Topological domaini252 – 260CytoplasmicSequence analysis9
Transmembranei261 – 280Helical; Name=M7Sequence analysisAdd BLAST20
Topological domaini281 – 298ExtracellularSequence analysisAdd BLAST18
Transmembranei299 – 319Helical; Name=M8Sequence analysisAdd BLAST21
Topological domaini320 – 342CytoplasmicSequence analysisAdd BLAST23
Transmembranei343 – 362Helical; Name=M9Sequence analysisAdd BLAST20
Topological domaini363 – 385ExtracellularSequence analysisAdd BLAST23
Intramembranei386 – 406Name=H10By similarityAdd BLAST21
Topological domaini407 – 414ExtracellularSequence analysis8
Transmembranei415 – 434Helical; Name=M10Sequence analysisAdd BLAST20
Topological domaini435 – 452CytoplasmicSequence analysisAdd BLAST18
Transmembranei453 – 474Helical; Name=M11Sequence analysisAdd BLAST22
Topological domaini475 – 483ExtracellularSequence analysis9
Transmembranei484 – 503Helical; Name=M12Sequence analysisAdd BLAST20
Topological domaini504 – 820CytoplasmicSequence analysisAdd BLAST317

Keywords - Cellular componenti

Cell membrane, Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000523481 – 820Sodium/hydrogen exchanger 1Add BLAST820

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi374N-linked (GlcNAc...) asparagineSequence analysis1
Modified residuei603PhosphoserineCombined sources1
Modified residuei606PhosphoserineCombined sources1
Modified residuei607PhosphothreonineCombined sources1
Modified residuei609PhosphoserineCombined sources1
Modified residuei697PhosphoserineCombined sources1
Modified residuei701PhosphoserineCombined sources1
Modified residuei707PhosphoserineCombined sources1
Modified residuei727PhosphoserineCombined sources1
Modified residuei730PhosphoserineCombined sources1
Modified residuei733PhosphoserineCombined sources1
Modified residuei755PhosphothreonineCombined sources1
Modified residuei790PhosphoserineCombined sources1
Modified residuei792PhosphoserineCombined sources1
Modified residuei801PhosphoserineBy similarity1

Post-translational modificationi

O-glycosylated.By similarity
Ubiquitinated, leading to its degradation by the proteasome. Ubiquitination is reduced by CHP1 (By similarity).By similarity

Keywords - PTMi

Glycoprotein, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiQ61165
PaxDbiQ61165
PRIDEiQ61165

PTM databases

iPTMnetiQ61165
PhosphoSitePlusiQ61165
SwissPalmiQ61165

Expressioni

Gene expression databases

BgeeiENSMUSG00000028854
ExpressionAtlasiQ61165 baseline and differential
GenevisibleiQ61165 MM

Interactioni

Subunit structurei

Oligomer. Interacts with calmodulin and TESC. Interacts (via the juxtamembrane region of the cytoplasmic C-terminal domain) with CHP1; the interaction occurs at the plasma membrane in a calcium-dependent manner. Interacts with CHP2; the interaction occurs in a calcium-dependent manner (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi203321, 1 interactor
IntActiQ61165, 1 interactor
STRINGi10090.ENSMUSP00000030669

Structurei

3D structure databases

ProteinModelPortaliQ61165
SMRiQ61165
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni520 – 543Interaction with CHP2By similarityAdd BLAST24

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1966 Eukaryota
COG0025 LUCA
GeneTreeiENSGT00760000119074
HOGENOMiHOG000247044
HOVERGENiHBG052615
InParanoidiQ61165
KOiK05742
OMAiYDRVGIV
OrthoDBiEOG091G02Q0
TreeFamiTF317212

Family and domain databases

InterProiView protein in InterPro
IPR006153 Cation/H_exchanger
IPR018422 Cation/H_exchanger_CPA1
IPR001970 Na/H_exchanger_1
IPR004709 NaH_exchanger
IPR032103 NHE_CaM-bd
PANTHERiPTHR10110 PTHR10110, 1 hit
PTHR10110:SF59 PTHR10110:SF59, 1 hit
PfamiView protein in Pfam
PF00999 Na_H_Exchanger, 1 hit
PF16644 NEXCaM_BD, 1 hit
PRINTSiPR01084 NAHEXCHNGR
PR01085 NAHEXCHNGR1
TIGRFAMsiTIGR00840 b_cpa1, 1 hit

Sequencei

Sequence statusi: Complete.

Q61165-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMLRWSGVWG FHPPRIFPSL LVVVALVGLL PVLRSHGLQH SPTASTIRGS
60 70 80 90 100
EPPRERSIGD VTTAPSEPLH RPDDHNLTNL IIEHGGKPSR KAFPVLDIDY
110 120 130 140 150
PHVRTPFEIS LWILLACLMK IGFHVIPTIS SIVPESCLLI VVGLLVGGLI
160 170 180 190 200
KGVGETPPFL QSDVFFLFLL PPIILDAGYF LPLRQFTENL GTILIFAVVG
210 220 230 240 250
TLWNAFFLGG LLYAVCLVGG EQINNIGLLD TLLFGSIISA VDPVAVLAVF
260 270 280 290 300
EEIHINELLH ILVFGESLLN DAVTVVLYHL FEEFASYDSV GISDIFLGFL
310 320 330 340 350
SFFVVALGGV FVGVVYGVIA AFTSRFTSHI RVIEPLFVFL YSYMAYLSAE
360 370 380 390 400
LFHLSGIMAL IASGVVMRPY VEANISHKSH TTIKYFLKMW SSVSETLIFI
410 420 430 440 450
FLGVSTVAGS HQWNWTFVIS TLLFCLIARV LGVLVLTWFI NKFRIVKLTP
460 470 480 490 500
KDQFIIAYGG LRGAIAFSLG YLLDKKHFPM CDLFLTAIIT VIFFTVFVQG
510 520 530 540 550
MTIRPLVDLL AVKKKQETKR SINEEIHTQF LDHLLTGIED ICGHYGHHHW
560 570 580 590 600
KDKLNRFNKK YVKKCLIAGE RSKEPQLIAF YHKMEMKQAI ELVESGGMGK
610 620 630 640 650
IPSAVSTVSM QNIHPKAVTS DRILPALSKD KEEEIRKILR SNLQKTRQRL
660 670 680 690 700
RSYNRHTLVA DPYEEAWNQM LLRRQKARQL EQKITNYLTV PAHKLDSPTL
710 720 730 740 750
SRARIGSDPL AYEPKADLPV ITIDPASPQS PESVDLVNEE LKGKVLGLNR
760 770 780 790 800
GPRVTPEEEE EDEDGIIMIR SKEPSSPGTD DVFTPGSSDS PSSQRIQRCL
810 820
SDPGPHPEPG EGEPFIPKGQ
Length:820
Mass (Da):91,468
Last modified:November 1, 1997 - v1
Checksum:i0589C4D08DD348BE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U51112 mRNA Translation: AAA92976.1
CCDSiCCDS38903.1
RefSeqiNP_058677.1, NM_016981.2
UniGeneiMm.4312

Genome annotation databases

EnsembliENSMUST00000030669; ENSMUSP00000030669; ENSMUSG00000028854
GeneIDi20544
KEGGimmu:20544
UCSCiuc008vcs.1 mouse

Entry informationi

Entry nameiSL9A1_MOUSE
AccessioniPrimary (citable) accession number: Q61165
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: May 23, 2018
This is version 158 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

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