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Q61165

- SL9A1_MOUSE

UniProt

Q61165 - SL9A1_MOUSE

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Protein

Sodium/hydrogen exchanger 1

Gene
Slc9a1, Nhe1
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Involved in pH regulation to eliminate acids generated by active metabolism or to counter adverse environmental conditions. Major proton extruding system driven by the inward sodium ion chemical gradient. Plays an important role in signal transduction.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei165 – 1651Channel pore-lining By similarity

GO - Molecular functioni

  1. sodium:proton antiporter activity Source: MGI

GO - Biological processi

  1. cardiac muscle cell differentiation Source: MGI
  2. cell growth Source: Ensembl
  3. cellular response to acidity Source: UniProtKB
  4. negative regulation of apoptotic process Source: Ensembl
  5. neuron death Source: Ensembl
  6. positive regulation of action potential Source: Ensembl
  7. positive regulation of cell growth Source: Ensembl
  8. positive regulation of mitochondrial membrane permeability Source: Ensembl
  9. protein oligomerization Source: UniProtKB
  10. regulation of intracellular pH Source: Ensembl
  11. regulation of pH Source: MGI
  12. regulation of sensory perception of pain Source: Ensembl
  13. regulation of the force of heart contraction Source: Ensembl
  14. response to acidity Source: MGI
  15. response to drug Source: Ensembl
  16. response to organic cyclic compound Source: Ensembl
  17. sodium ion export Source: UniProtKB
  18. sodium ion transport Source: MGI
Complete GO annotation...

Keywords - Biological processi

Antiport, Ion transport, Sodium transport, Transport

Keywords - Ligandi

Calmodulin-binding, Sodium

Enzyme and pathway databases

ReactomeiREACT_196554. Hyaluronan uptake and degradation.

Names & Taxonomyi

Protein namesi
Recommended name:
Sodium/hydrogen exchanger 1
Alternative name(s):
Na(+)/H(+) exchanger 1
Short name:
NHE-1
Solute carrier family 9 member 1
Gene namesi
Name:Slc9a1
Synonyms:Nhe1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 4

Organism-specific databases

MGIiMGI:102462. Slc9a1.

Subcellular locationi

Membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane; Multi-pass membrane protein By similarity. Cell membrane; Multi-pass membrane protein
Note: Colocalizes with CHP1 and CHP2 at the reticulum endoplasmic and plasma membrane By similarity.

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 1212Cytoplasmic Reviewed predictionAdd
BLAST
Transmembranei13 – 3321Helical; Name=M1; Reviewed predictionAdd
BLAST
Topological domaini34 – 10572Extracellular Reviewed predictionAdd
BLAST
Transmembranei106 – 12621Helical; Name=M2; Reviewed predictionAdd
BLAST
Topological domaini127 – 1337Cytoplasmic Reviewed prediction
Transmembranei134 – 15320Helical; Name=M3; Reviewed predictionAdd
BLAST
Topological domaini154 – 1585Extracellular Reviewed prediction
Transmembranei159 – 17820Helical; Name=M4; Reviewed predictionAdd
BLAST
Topological domaini179 – 19517Cytoplasmic Reviewed predictionAdd
BLAST
Transmembranei196 – 21520Helical; Name=M5; Reviewed predictionAdd
BLAST
Topological domaini216 – 23116Extracellular Reviewed predictionAdd
BLAST
Transmembranei232 – 25120Helical; Name=M6; Reviewed predictionAdd
BLAST
Topological domaini252 – 2609Cytoplasmic Reviewed prediction
Transmembranei261 – 28020Helical; Name=M7; Reviewed predictionAdd
BLAST
Topological domaini281 – 29818Extracellular Reviewed predictionAdd
BLAST
Transmembranei299 – 31921Helical; Name=M8; Reviewed predictionAdd
BLAST
Topological domaini320 – 34223Cytoplasmic Reviewed predictionAdd
BLAST
Transmembranei343 – 36220Helical; Name=M9; Reviewed predictionAdd
BLAST
Topological domaini363 – 38523Extracellular Reviewed predictionAdd
BLAST
Intramembranei386 – 40621Name=H10; By similarityAdd
BLAST
Topological domaini407 – 4148Extracellular Reviewed prediction
Transmembranei415 – 43420Helical; Name=M10; Reviewed predictionAdd
BLAST
Topological domaini435 – 45218Cytoplasmic Reviewed predictionAdd
BLAST
Transmembranei453 – 47422Helical; Name=M11; Reviewed predictionAdd
BLAST
Topological domaini475 – 4839Extracellular Reviewed prediction
Transmembranei484 – 50320Helical; Name=M12; Reviewed predictionAdd
BLAST
Topological domaini504 – 820317Cytoplasmic Reviewed predictionAdd
BLAST

GO - Cellular componenti

  1. apical plasma membrane Source: MGI
  2. basolateral plasma membrane Source: MGI
  3. cytoplasm Source: UniProtKB
  4. endoplasmic reticulum Source: UniProtKB
  5. endoplasmic reticulum membrane Source: UniProtKB-SubCell
  6. integral component of membrane Source: UniProtKB-KW
  7. membrane Source: MGI
  8. membrane raft Source: Ensembl
  9. mitochondrion Source: Ensembl
  10. plasma membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 820820Sodium/hydrogen exchanger 1PRO_0000052348Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi374 – 3741N-linked (GlcNAc...) Reviewed prediction
Modified residuei603 – 6031Phosphoserine1 Publication
Modified residuei609 – 6091Phosphoserine1 Publication
Modified residuei697 – 6971Phosphoserine2 Publications
Modified residuei701 – 7011Phosphoserine By similarity
Modified residuei707 – 7071Phosphoserine2 Publications
Modified residuei727 – 7271Phosphoserine By similarity
Modified residuei730 – 7301Phosphoserine By similarity
Modified residuei733 – 7331Phosphoserine By similarity
Modified residuei790 – 7901Phosphoserine By similarity

Post-translational modificationi

O-glycosylated By similarity.
Ubiquitinated, leading to its degradation by the proteasome. Ubiquitination is reduced by CHP1 By similarity.

Keywords - PTMi

Glycoprotein, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiQ61165.
PaxDbiQ61165.
PRIDEiQ61165.

PTM databases

PhosphoSiteiQ61165.

Expressioni

Gene expression databases

ArrayExpressiQ61165.
BgeeiQ61165.
GenevestigatoriQ61165.

Interactioni

Subunit structurei

Oligomer. Interacts with calmodulin and TESC. Interacts (via the juxtamembrane region of the cytoplasmic C-terminal domain) with CHP1; the interaction occurs at the plasma membrane in a calcium-dependent manner. Interacts with CHP2; the interaction occurs in a calcium-dependent manner By similarity.

Protein-protein interaction databases

BioGridi203321. 3 interactions.
IntActiQ61165. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliQ61165.
SMRiQ61165. Positions 159-184, 342-369, 451-476, 507-549, 626-689.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni520 – 54324Interaction with CHP2 By similarityAdd
BLAST

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0025.
GeneTreeiENSGT00560000077158.
HOGENOMiHOG000247044.
HOVERGENiHBG052615.
KOiK05742.
OMAiYDRVGIV.
OrthoDBiEOG7XWPN4.
TreeFamiTF317212.

Family and domain databases

InterProiIPR006153. Cation/H_exchanger.
IPR018422. Cation/H_exchanger_CPA1.
IPR001970. Na/H_exchanger_1.
IPR004709. NaH_exchanger.
[Graphical view]
PANTHERiPTHR10110. PTHR10110. 1 hit.
PfamiPF00999. Na_H_Exchanger. 1 hit.
[Graphical view]
PRINTSiPR01084. NAHEXCHNGR.
PR01085. NAHEXCHNGR1.
TIGRFAMsiTIGR00840. b_cpa1. 1 hit.

Sequencei

Sequence statusi: Complete.

Q61165-1 [UniParc]FASTAAdd to Basket

« Hide

MMLRWSGVWG FHPPRIFPSL LVVVALVGLL PVLRSHGLQH SPTASTIRGS    50
EPPRERSIGD VTTAPSEPLH RPDDHNLTNL IIEHGGKPSR KAFPVLDIDY 100
PHVRTPFEIS LWILLACLMK IGFHVIPTIS SIVPESCLLI VVGLLVGGLI 150
KGVGETPPFL QSDVFFLFLL PPIILDAGYF LPLRQFTENL GTILIFAVVG 200
TLWNAFFLGG LLYAVCLVGG EQINNIGLLD TLLFGSIISA VDPVAVLAVF 250
EEIHINELLH ILVFGESLLN DAVTVVLYHL FEEFASYDSV GISDIFLGFL 300
SFFVVALGGV FVGVVYGVIA AFTSRFTSHI RVIEPLFVFL YSYMAYLSAE 350
LFHLSGIMAL IASGVVMRPY VEANISHKSH TTIKYFLKMW SSVSETLIFI 400
FLGVSTVAGS HQWNWTFVIS TLLFCLIARV LGVLVLTWFI NKFRIVKLTP 450
KDQFIIAYGG LRGAIAFSLG YLLDKKHFPM CDLFLTAIIT VIFFTVFVQG 500
MTIRPLVDLL AVKKKQETKR SINEEIHTQF LDHLLTGIED ICGHYGHHHW 550
KDKLNRFNKK YVKKCLIAGE RSKEPQLIAF YHKMEMKQAI ELVESGGMGK 600
IPSAVSTVSM QNIHPKAVTS DRILPALSKD KEEEIRKILR SNLQKTRQRL 650
RSYNRHTLVA DPYEEAWNQM LLRRQKARQL EQKITNYLTV PAHKLDSPTL 700
SRARIGSDPL AYEPKADLPV ITIDPASPQS PESVDLVNEE LKGKVLGLNR 750
GPRVTPEEEE EDEDGIIMIR SKEPSSPGTD DVFTPGSSDS PSSQRIQRCL 800
SDPGPHPEPG EGEPFIPKGQ 820
Length:820
Mass (Da):91,468
Last modified:November 1, 1997 - v1
Checksum:i0589C4D08DD348BE
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U51112 mRNA. Translation: AAA92976.1.
CCDSiCCDS38903.1.
RefSeqiNP_058677.1. NM_016981.2.
UniGeneiMm.4312.

Genome annotation databases

EnsembliENSMUST00000030669; ENSMUSP00000030669; ENSMUSG00000028854.
GeneIDi20544.
KEGGimmu:20544.
UCSCiuc008vcs.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U51112 mRNA. Translation: AAA92976.1 .
CCDSi CCDS38903.1.
RefSeqi NP_058677.1. NM_016981.2.
UniGenei Mm.4312.

3D structure databases

ProteinModelPortali Q61165.
SMRi Q61165. Positions 159-184, 342-369, 451-476, 507-549, 626-689.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 203321. 3 interactions.
IntActi Q61165. 1 interaction.

PTM databases

PhosphoSitei Q61165.

Proteomic databases

MaxQBi Q61165.
PaxDbi Q61165.
PRIDEi Q61165.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000030669 ; ENSMUSP00000030669 ; ENSMUSG00000028854 .
GeneIDi 20544.
KEGGi mmu:20544.
UCSCi uc008vcs.1. mouse.

Organism-specific databases

CTDi 6548.
MGIi MGI:102462. Slc9a1.

Phylogenomic databases

eggNOGi COG0025.
GeneTreei ENSGT00560000077158.
HOGENOMi HOG000247044.
HOVERGENi HBG052615.
KOi K05742.
OMAi YDRVGIV.
OrthoDBi EOG7XWPN4.
TreeFami TF317212.

Enzyme and pathway databases

Reactomei REACT_196554. Hyaluronan uptake and degradation.

Miscellaneous databases

NextBioi 298831.
PROi Q61165.
SOURCEi Search...

Gene expression databases

ArrayExpressi Q61165.
Bgeei Q61165.
Genevestigatori Q61165.

Family and domain databases

InterProi IPR006153. Cation/H_exchanger.
IPR018422. Cation/H_exchanger_CPA1.
IPR001970. Na/H_exchanger_1.
IPR004709. NaH_exchanger.
[Graphical view ]
PANTHERi PTHR10110. PTHR10110. 1 hit.
Pfami PF00999. Na_H_Exchanger. 1 hit.
[Graphical view ]
PRINTSi PR01084. NAHEXCHNGR.
PR01085. NAHEXCHNGR1.
TIGRFAMsi TIGR00840. b_cpa1. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "cDNA cloning and expression of the mouse Na/H antiporter (NHE-1) and a potential splice variant."
    Dewey M.J., Ennis T.M., Bowman L.H.
    Mol. Biol. Rep. 28:111-117(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: BALB/c.
  2. "Qualitative and quantitative analyses of protein phosphorylation in naive and stimulated mouse synaptosomal preparations."
    Munton R.P., Tweedie-Cullen R., Livingstone-Zatchej M., Weinandy F., Waidelich M., Longo D., Gehrig P., Potthast F., Rutishauser D., Gerrits B., Panse C., Schlapbach R., Mansuy I.M.
    Mol. Cell. Proteomics 6:283-293(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain cortex.
  3. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-603; SER-609 AND SER-707, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.
  4. "The phagosomal proteome in interferon-gamma-activated macrophages."
    Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
    Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-697 AND SER-707, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  5. "Large scale localization of protein phosphorylation by use of electron capture dissociation mass spectrometry."
    Sweet S.M., Bailey C.M., Cunningham D.L., Heath J.K., Cooper H.J.
    Mol. Cell. Proteomics 8:904-912(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-697, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Embryonic fibroblast.

Entry informationi

Entry nameiSL9A1_MOUSE
AccessioniPrimary (citable) accession number: Q61165
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: September 3, 2014
This is version 127 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Caution

The region between transmembrane regions M4 and M5 and between M6 and M7 (also termed intracellular loops IL2 and IL4, respectively) seem to be localized at least in part in the membrane. The hydrophobic region H10 is proposed to be located within the membrane.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi