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Q61165 (SL9A1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 126. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Sodium/hydrogen exchanger 1
Alternative name(s):
Na(+)/H(+) exchanger 1
Short name=NHE-1
Solute carrier family 9 member 1
Gene names
Name:Slc9a1
Synonyms:Nhe1
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length820 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in pH regulation to eliminate acids generated by active metabolism or to counter adverse environmental conditions. Major proton extruding system driven by the inward sodium ion chemical gradient. Plays an important role in signal transduction.

Subunit structure

Oligomer. Interacts with calmodulin and TESC. Interacts (via the juxtamembrane region of the cytoplasmic C-terminal domain) with CHP1; the interaction occurs at the plasma membrane in a calcium-dependent manner. Interacts with CHP2; the interaction occurs in a calcium-dependent manner By similarity.

Subcellular location

Membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane; Multi-pass membrane protein By similarity. Cell membrane; Multi-pass membrane protein. Note: Colocalizes with CHP1 and CHP2 at the reticulum endoplasmic and plasma membrane By similarity.

Post-translational modification

O-glycosylated By similarity.

Ubiquitinated, leading to its degradation by the proteasome. Ubiquitination is reduced by CHP1 By similarity.

Sequence similarities

Belongs to the monovalent cation:proton antiporter 1 (CPA1) transporter (TC 2.A.36) family. [View classification]

Caution

The region between transmembrane regions M4 and M5 and between M6 and M7 (also termed intracellular loops IL2 and IL4, respectively) seem to be localized at least in part in the membrane. The hydrophobic region H10 is proposed to be located within the membrane.

Ontologies

Keywords
   Biological processAntiport
Ion transport
Sodium transport
Transport
   Cellular componentCell membrane
Endoplasmic reticulum
Membrane
   DomainTransmembrane
Transmembrane helix
   LigandCalmodulin-binding
Sodium
   PTMGlycoprotein
Phosphoprotein
Ubl conjugation
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcardiac muscle cell differentiation

Inferred from mutant phenotype PubMed 19011045. Source: MGI

cell growth

Inferred from electronic annotation. Source: Ensembl

cellular response to acidity

Inferred from sequence or structural similarity. Source: UniProtKB

negative regulation of apoptotic process

Inferred from electronic annotation. Source: Ensembl

neuron death

Inferred from electronic annotation. Source: Ensembl

positive regulation of action potential

Inferred from electronic annotation. Source: Ensembl

positive regulation of cell growth

Inferred from electronic annotation. Source: Ensembl

positive regulation of mitochondrial membrane permeability

Inferred from electronic annotation. Source: Ensembl

protein oligomerization

Inferred from sequence or structural similarity. Source: UniProtKB

regulation of intracellular pH

Inferred from electronic annotation. Source: Ensembl

regulation of pH

Inferred from direct assay PubMed 10666043. Source: MGI

regulation of sensory perception of pain

Inferred from electronic annotation. Source: Ensembl

regulation of the force of heart contraction

Inferred from electronic annotation. Source: Ensembl

response to acidity

Inferred from mutant phenotype PubMed 10673550. Source: MGI

response to drug

Inferred from electronic annotation. Source: Ensembl

response to organic cyclic compound

Inferred from electronic annotation. Source: Ensembl

sodium ion export

Inferred from sequence or structural similarity. Source: UniProtKB

sodium ion transport

Inferred from direct assay PubMed 10666043. Source: MGI

   Cellular_componentapical plasma membrane

Inferred from direct assay PubMed 21705333. Source: MGI

basolateral plasma membrane

Traceable author statement PubMed 10666043. Source: MGI

cytoplasm

Inferred from sequence or structural similarity. Source: UniProtKB

endoplasmic reticulum

Inferred from sequence or structural similarity. Source: UniProtKB

endoplasmic reticulum membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

integral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

membrane

Inferred from direct assay PubMed 12619893. Source: MGI

membrane raft

Inferred from electronic annotation. Source: Ensembl

mitochondrion

Inferred from electronic annotation. Source: Ensembl

plasma membrane

Inferred from sequence or structural similarity. Source: UniProtKB

   Molecular_functionsodium:proton antiporter activity

Inferred from direct assay PubMed 10666043. Source: MGI

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 820820Sodium/hydrogen exchanger 1
PRO_0000052348

Regions

Topological domain1 – 1212Cytoplasmic Potential
Transmembrane13 – 3321Helical; Name=M1; Potential
Topological domain34 – 10572Extracellular Potential
Transmembrane106 – 12621Helical; Name=M2; Potential
Topological domain127 – 1337Cytoplasmic Potential
Transmembrane134 – 15320Helical; Name=M3; Potential
Topological domain154 – 1585Extracellular Potential
Transmembrane159 – 17820Helical; Name=M4; Potential
Topological domain179 – 19517Cytoplasmic Potential
Transmembrane196 – 21520Helical; Name=M5; Potential
Topological domain216 – 23116Extracellular Potential
Transmembrane232 – 25120Helical; Name=M6; Potential
Topological domain252 – 2609Cytoplasmic Potential
Transmembrane261 – 28020Helical; Name=M7; Potential
Topological domain281 – 29818Extracellular Potential
Transmembrane299 – 31921Helical; Name=M8; Potential
Topological domain320 – 34223Cytoplasmic Potential
Transmembrane343 – 36220Helical; Name=M9; Potential
Topological domain363 – 38523Extracellular Potential
Intramembrane386 – 40621Name=H10; By similarity
Topological domain407 – 4148Extracellular Potential
Transmembrane415 – 43420Helical; Name=M10; Potential
Topological domain435 – 45218Cytoplasmic Potential
Transmembrane453 – 47422Helical; Name=M11; Potential
Topological domain475 – 4839Extracellular Potential
Transmembrane484 – 50320Helical; Name=M12; Potential
Topological domain504 – 820317Cytoplasmic Potential
Region520 – 54324Interaction with CHP2 By similarity

Sites

Site1651Channel pore-lining By similarity

Amino acid modifications

Modified residue6031Phosphoserine Ref.3
Modified residue6091Phosphoserine Ref.3
Modified residue6971Phosphoserine Ref.4 Ref.5
Modified residue7011Phosphoserine By similarity
Modified residue7071Phosphoserine Ref.3 Ref.4
Modified residue7271Phosphoserine By similarity
Modified residue7301Phosphoserine By similarity
Modified residue7331Phosphoserine By similarity
Modified residue7901Phosphoserine By similarity
Glycosylation3741N-linked (GlcNAc...) Potential

Sequences

Sequence LengthMass (Da)Tools
Q61165 [UniParc].

Last modified November 1, 1997. Version 1.
Checksum: 0589C4D08DD348BE

FASTA82091,468
        10         20         30         40         50         60 
MMLRWSGVWG FHPPRIFPSL LVVVALVGLL PVLRSHGLQH SPTASTIRGS EPPRERSIGD 

        70         80         90        100        110        120 
VTTAPSEPLH RPDDHNLTNL IIEHGGKPSR KAFPVLDIDY PHVRTPFEIS LWILLACLMK 

       130        140        150        160        170        180 
IGFHVIPTIS SIVPESCLLI VVGLLVGGLI KGVGETPPFL QSDVFFLFLL PPIILDAGYF 

       190        200        210        220        230        240 
LPLRQFTENL GTILIFAVVG TLWNAFFLGG LLYAVCLVGG EQINNIGLLD TLLFGSIISA 

       250        260        270        280        290        300 
VDPVAVLAVF EEIHINELLH ILVFGESLLN DAVTVVLYHL FEEFASYDSV GISDIFLGFL 

       310        320        330        340        350        360 
SFFVVALGGV FVGVVYGVIA AFTSRFTSHI RVIEPLFVFL YSYMAYLSAE LFHLSGIMAL 

       370        380        390        400        410        420 
IASGVVMRPY VEANISHKSH TTIKYFLKMW SSVSETLIFI FLGVSTVAGS HQWNWTFVIS 

       430        440        450        460        470        480 
TLLFCLIARV LGVLVLTWFI NKFRIVKLTP KDQFIIAYGG LRGAIAFSLG YLLDKKHFPM 

       490        500        510        520        530        540 
CDLFLTAIIT VIFFTVFVQG MTIRPLVDLL AVKKKQETKR SINEEIHTQF LDHLLTGIED 

       550        560        570        580        590        600 
ICGHYGHHHW KDKLNRFNKK YVKKCLIAGE RSKEPQLIAF YHKMEMKQAI ELVESGGMGK 

       610        620        630        640        650        660 
IPSAVSTVSM QNIHPKAVTS DRILPALSKD KEEEIRKILR SNLQKTRQRL RSYNRHTLVA 

       670        680        690        700        710        720 
DPYEEAWNQM LLRRQKARQL EQKITNYLTV PAHKLDSPTL SRARIGSDPL AYEPKADLPV 

       730        740        750        760        770        780 
ITIDPASPQS PESVDLVNEE LKGKVLGLNR GPRVTPEEEE EDEDGIIMIR SKEPSSPGTD 

       790        800        810        820 
DVFTPGSSDS PSSQRIQRCL SDPGPHPEPG EGEPFIPKGQ 

« Hide

References

« Hide 'large scale' references
[1]"cDNA cloning and expression of the mouse Na/H antiporter (NHE-1) and a potential splice variant."
Dewey M.J., Ennis T.M., Bowman L.H.
Mol. Biol. Rep. 28:111-117(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: BALB/c.
[2]"Qualitative and quantitative analyses of protein phosphorylation in naive and stimulated mouse synaptosomal preparations."
Munton R.P., Tweedie-Cullen R., Livingstone-Zatchej M., Weinandy F., Waidelich M., Longo D., Gehrig P., Potthast F., Rutishauser D., Gerrits B., Panse C., Schlapbach R., Mansuy I.M.
Mol. Cell. Proteomics 6:283-293(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Brain cortex.
[3]"Large-scale phosphorylation analysis of mouse liver."
Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-603; SER-609 AND SER-707, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Liver.
[4]"The phagosomal proteome in interferon-gamma-activated macrophages."
Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-697 AND SER-707, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[5]"Large scale localization of protein phosphorylation by use of electron capture dissociation mass spectrometry."
Sweet S.M., Bailey C.M., Cunningham D.L., Heath J.K., Cooper H.J.
Mol. Cell. Proteomics 8:904-912(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-697, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Embryonic fibroblast.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U51112 mRNA. Translation: AAA92976.1.
CCDSCCDS38903.1.
RefSeqNP_058677.1. NM_016981.2.
UniGeneMm.4312.

3D structure databases

ProteinModelPortalQ61165.
SMRQ61165. Positions 159-184, 342-369, 451-476, 507-549, 626-689.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid203321. 3 interactions.
IntActQ61165. 1 interaction.

PTM databases

PhosphoSiteQ61165.

Proteomic databases

MaxQBQ61165.
PaxDbQ61165.
PRIDEQ61165.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000030669; ENSMUSP00000030669; ENSMUSG00000028854.
GeneID20544.
KEGGmmu:20544.
UCSCuc008vcs.1. mouse.

Organism-specific databases

CTD6548.
MGIMGI:102462. Slc9a1.

Phylogenomic databases

eggNOGCOG0025.
GeneTreeENSGT00560000077158.
HOGENOMHOG000247044.
HOVERGENHBG052615.
KOK05742.
OMAYDRVGIV.
OrthoDBEOG7XWPN4.
TreeFamTF317212.

Gene expression databases

ArrayExpressQ61165.
BgeeQ61165.
GenevestigatorQ61165.

Family and domain databases

InterProIPR006153. Cation/H_exchanger.
IPR018422. Cation/H_exchanger_CPA1.
IPR001970. Na/H_exchanger_1.
IPR004709. NaH_exchanger.
[Graphical view]
PANTHERPTHR10110. PTHR10110. 1 hit.
PfamPF00999. Na_H_Exchanger. 1 hit.
[Graphical view]
PRINTSPR01084. NAHEXCHNGR.
PR01085. NAHEXCHNGR1.
TIGRFAMsTIGR00840. b_cpa1. 1 hit.
ProtoNetSearch...

Other

NextBio298831.
PROQ61165.
SOURCESearch...

Entry information

Entry nameSL9A1_MOUSE
AccessionPrimary (citable) accession number: Q61165
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: July 9, 2014
This is version 126 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot