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Protein

Type I iodothyronine deiodinase

Gene

Dio1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Responsible for the deiodination of T4 (3,5,3',5'-tetraiodothyronine) into T3 (3,5,3'-triiodothyronine) and of T3 into T2 (3,3'-diiodothyronine).

Catalytic activityi

3,5,3'-triiodo-L-thyronine + iodide + A + H+ = L-thyroxine + AH2.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei126 – 1261

GO - Molecular functioni

  1. selenium binding Source: MGI
  2. thyroxine 5'-deiodinase activity Source: UniProtKB

GO - Biological processi

  1. hormone biosynthetic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Thyroid hormones biosynthesis

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-16138.
BRENDAi1.97.1.10. 3474.
ReactomeiREACT_294831. Regulation of thyroid hormone activity.
SABIO-RKQ61153.

Names & Taxonomyi

Protein namesi
Recommended name:
Type I iodothyronine deiodinase (EC:1.97.1.10)
Alternative name(s):
5DI
DIOI
Type 1 DI
Type-I 5'-deiodinase
Gene namesi
Name:Dio1
Synonyms:Itdi1, Txdi1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:94896. Dio1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei13 – 3321HelicalSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. endoplasmic reticulum membrane Source: UniProtKB-SubCell
  2. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 257257Type I iodothyronine deiodinasePRO_0000154312Add
BLAST

Proteomic databases

MaxQBiQ61153.
PaxDbiQ61153.
PRIDEiQ61153.

Expressioni

Gene expression databases

BgeeiQ61153.
CleanExiMM_DIO1.
ExpressionAtlasiQ61153. baseline and differential.
GenevestigatoriQ61153.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000081007.

Structurei

3D structure databases

ProteinModelPortaliQ61153.
SMRiQ61153. Positions 85-246.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the iodothyronine deiodinase family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG67608.
HOGENOMiHOG000169547.
HOVERGENiHBG000099.
InParanoidiQ61153.
KOiK01562.
OrthoDBiEOG751NHS.
PhylomeDBiQ61153.
TreeFamiTF329721.

Family and domain databases

Gene3Di3.40.30.10. 1 hit.
InterProiIPR000643. Iodothyronine_deiodinase.
IPR008261. Iodothyronine_deiodinase_AS.
IPR027252. Iodothyronine_deiodinase_I/III.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PANTHERiPTHR11781. PTHR11781. 1 hit.
PfamiPF00837. T4_deiodinase. 1 hit.
[Graphical view]
PIRSFiPIRSF001330. IOD. 1 hit.
PIRSF500144. IODI_III. 1 hit.
SUPFAMiSSF52833. SSF52833. 1 hit.
PROSITEiPS01205. T4_DEIODINASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q61153-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGLPQLWLWL KRLVIFLQVA LEVAVGKVLM TLFPGRVKQS ILAMGQKTGM
60 70 80 90 100
ARNPRFAPDN WVPTFFSIQY FWFVLKVRWQ RLEDRAEFGG LAPNCTVVCL
110 120 130 140 150
SGQKCNIWDF IQGSRPLVLN FGSCTUPSFL LKFDQFKRLV DDFASTADFL
160 170 180 190 200
IIYIEEAHAT DGWAFKNNVD IRQHRSLQER VRAARLLLAR SPQCPVVVDT
210 220 230 240 250
MQNQSSQLYA ALPERLYVIQ EGRICYKGKA GPWNYNPEEV RAVLEKLCTP

PRHVPQL
Length:257
Mass (Da):29,568
Last modified:February 26, 2008 - v3
Checksum:iDF569BDFF7A4257A
GO

Sequence cautioni

The sequence AAB00228.1 differs from that shown. Reason: Erroneous termination at position 126. Translated as Sec.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti167 – 1671N → K in AAB00228 (PubMed:7476994).Curated
Sequence conflicti171 – 1711I → V in AAB00228 (PubMed:7476994).Curated
Sequence conflicti186 – 1861L → M in AAB00228 (PubMed:7476994).Curated
Sequence conflicti186 – 1861L → M in AAH89608 (PubMed:15489334).Curated
Sequence conflicti193 – 1931Q → R in AAH19786 (PubMed:15489334).Curated
Sequence conflicti225 – 2251C → R in AAH19786 (PubMed:15489334).Curated
Sequence conflicti240 – 2401V → L in AAB00228 (PubMed:7476994).Curated

Non-standard residue

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Non-standard residuei126 – 1261Selenocysteine

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U49861 mRNA. Translation: AAB00228.1. Sequence problems.
BC019786 mRNA. Translation: AAH19786.1.
BC089608 mRNA. Translation: AAH89608.1.
CCDSiCCDS18436.1.
RefSeqiNP_031886.3. NM_007860.3.
UniGeneiMm.148342.

Genome annotation databases

GeneIDi13370.
KEGGimmu:13370.
UCSCiuc008tzq.1. mouse.

Keywords - Coding sequence diversityi

Selenocysteine

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U49861 mRNA. Translation: AAB00228.1. Sequence problems.
BC019786 mRNA. Translation: AAH19786.1.
BC089608 mRNA. Translation: AAH89608.1.
CCDSiCCDS18436.1.
RefSeqiNP_031886.3. NM_007860.3.
UniGeneiMm.148342.

3D structure databases

ProteinModelPortaliQ61153.
SMRiQ61153. Positions 85-246.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000081007.

Proteomic databases

MaxQBiQ61153.
PaxDbiQ61153.
PRIDEiQ61153.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi13370.
KEGGimmu:13370.
UCSCiuc008tzq.1. mouse.

Organism-specific databases

CTDi1733.
MGIiMGI:94896. Dio1.

Phylogenomic databases

eggNOGiNOG67608.
HOGENOMiHOG000169547.
HOVERGENiHBG000099.
InParanoidiQ61153.
KOiK01562.
OrthoDBiEOG751NHS.
PhylomeDBiQ61153.
TreeFamiTF329721.

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-16138.
BRENDAi1.97.1.10. 3474.
ReactomeiREACT_294831. Regulation of thyroid hormone activity.
SABIO-RKQ61153.

Miscellaneous databases

ChiTaRSiDio1. mouse.
NextBioi283716.
PROiQ61153.
SOURCEiSearch...

Gene expression databases

BgeeiQ61153.
CleanExiMM_DIO1.
ExpressionAtlasiQ61153. baseline and differential.
GenevestigatoriQ61153.

Family and domain databases

Gene3Di3.40.30.10. 1 hit.
InterProiIPR000643. Iodothyronine_deiodinase.
IPR008261. Iodothyronine_deiodinase_AS.
IPR027252. Iodothyronine_deiodinase_I/III.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PANTHERiPTHR11781. PTHR11781. 1 hit.
PfamiPF00837. T4_deiodinase. 1 hit.
[Graphical view]
PIRSFiPIRSF001330. IOD. 1 hit.
PIRSF500144. IODI_III. 1 hit.
SUPFAMiSSF52833. SSF52833. 1 hit.
PROSITEiPS01205. T4_DEIODINASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Structural and functional differences in the dio1 gene in mice with inherited type 1 deiodinase deficiency."
    Maia A.L., Berry M.J., Sabbag R., Harney J.W., Larsen P.R.
    Mol. Endocrinol. 9:969-980(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: C57BL/6J.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Liver.

Entry informationi

Entry nameiIOD1_MOUSE
AccessioniPrimary (citable) accession number: Q61153
Secondary accession number(s): Q05CY5, Q5FW63
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: February 26, 2008
Last modified: April 1, 2015
This is version 115 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.