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Protein

Occludin

Gene

Ocln

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May play a role in the formation and regulation of the tight junction (TJ) paracellular permeability barrier.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Occludin
Gene namesi
Name:Ocln
Synonyms:Ocl
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 13

Organism-specific databases

MGIiMGI:106183. Ocln.

Subcellular locationi

  • Cell membrane By similarity; Multi-pass membrane protein Sequence analysis
  • Cell junctiontight junction By similarity

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 66CytoplasmicSequence analysisAdd BLAST66
Transmembranei67 – 89HelicalSequence analysisAdd BLAST23
Topological domaini90 – 133ExtracellularSequence analysisAdd BLAST44
Transmembranei134 – 158HelicalSequence analysisAdd BLAST25
Topological domaini159 – 168CytoplasmicSequence analysis10
Transmembranei169 – 193HelicalSequence analysisAdd BLAST25
Topological domaini194 – 241ExtracellularSequence analysisAdd BLAST48
Transmembranei242 – 263HelicalSequence analysisAdd BLAST22
Topological domaini264 – 521CytoplasmicSequence analysisAdd BLAST258

GO - Cellular componenti

  • apical plasma membrane Source: MGI
  • apicolateral plasma membrane Source: MGI
  • bicellular tight junction Source: UniProtKB
  • cell-cell junction Source: MGI
  • cell junction Source: MGI
  • cell surface Source: MGI
  • cytoplasmic vesicle Source: MGI
  • endocytic vesicle Source: UniProtKB
  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Tight junction

Pathology & Biotechi

Disruption phenotypei

Mice have a complex phenotype including abnormalities of salivary gland, gastric epithelium, bone, testis and intracranial calcification.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001467401 – 521OccludinAdd BLAST521

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei300PhosphoserineCombined sources1
Modified residuei303PhosphothreonineCombined sources1
Modified residuei311PhosphoserineCombined sources1
Modified residuei319PhosphoserineBy similarity1
Modified residuei338Phosphoserine; by PKC; in vitro1 Publication1
Modified residuei358PhosphoserineCombined sources1
Modified residuei367PhosphotyrosineBy similarity1
Modified residuei368PhosphoserineCombined sources1
Modified residuei369PhosphoserineCombined sources1
Modified residuei397PhosphotyrosineBy similarity1
Modified residuei401PhosphotyrosineBy similarity1
Modified residuei402Phosphothreonine; by PKC/PRKCHBy similarity1
Modified residuei403PhosphothreonineCombined sources1
Modified residuei407PhosphoserineCombined sources1
Modified residuei489PhosphoserineBy similarity1

Post-translational modificationi

Dephosphorylated by PTPRJ (By similarity). May be phosphorylated by PKC during translocation to cell-cell contacts.By similarity1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ61146.
PaxDbiQ61146.
PRIDEiQ61146.

PTM databases

iPTMnetiQ61146.
PhosphoSitePlusiQ61146.

Expressioni

Tissue specificityi

Localized at tight junctions of both epithelial and endothelial cells. Highly expressed in the testis, kidney, lung, liver and brain. Not detected in skeletal muscle, spleen and heart.

Developmental stagei

Found diffusely on the lateral membranes of Sertoli cells in the early prepubertal period. With development, became gradually concentrated at the most basal regions of Sertoli cells.

Gene expression databases

BgeeiENSMUSG00000021638.
CleanExiMM_OCLN.
ExpressionAtlasiQ61146. baseline and differential.
GenevisibleiQ61146. MM.

Interactioni

Subunit structurei

Interacts with TJP1/ZO1 and with VAPA.By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi201894. 6 interactors.
DIPiDIP-49005N.
STRINGi10090.ENSMUSP00000022140.

Structurei

3D structure databases

ProteinModelPortaliQ61146.
SMRiQ61146.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini60 – 267MARVELPROSITE-ProRule annotationAdd BLAST208

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili424 – 488Sequence analysisAdd BLAST65

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi92 – 129Gly/Tyr-richAdd BLAST38
Compositional biasi307 – 310Poly-Pro4

Domaini

The C-terminal is cytoplasmic and is important for interaction with ZO-1. Necessary for the tight junction localization. Involved in the regulation of the permeability barrier function of the tight junction (By similarity).By similarity

Sequence similaritiesi

Belongs to the ELL/occludin family.Curated
Contains 1 MARVEL domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IGK8. Eukaryota.
ENOG4110E0E. LUCA.
GeneTreeiENSGT00730000110989.
HOGENOMiHOG000233490.
HOVERGENiHBG004523.
InParanoidiQ61146.
KOiK06088.
OMAiYAPSNDV.
OrthoDBiEOG091G0BWN.
PhylomeDBiQ61146.
TreeFamiTF326161.

Family and domain databases

InterProiIPR031176. ELL/occludin.
IPR008253. Marvel.
IPR002958. Occludin.
IPR010844. Occludin_ELL.
[Graphical view]
PANTHERiPTHR23288. PTHR23288. 1 hit.
PTHR23288:SF4. PTHR23288:SF4. 1 hit.
PfamiPF01284. MARVEL. 1 hit.
PF07303. Occludin_ELL. 1 hit.
[Graphical view]
PIRSFiPIRSF005993. Occludin. 1 hit.
PRINTSiPR01258. OCCLUDIN.
PROSITEiPS51225. MARVEL. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q61146-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSVRPFESPP PYRPDEFKPN HYAPSNDMYG GEMHVRPMLS QPAYSFYPED
60 70 80 90 100
EILHFYKWTS PPGVIRILSM LIIVMCIAIF ACVASTLAWD RGYGTGLFGG
110 120 130 140 150
SLNYPYSGFG YGGGYGGGYG GYGYGYGGYT DPRAAKGFLL AMAAFCFIAS
160 170 180 190 200
LVIFVTSVIR SGMSRTRRYY LIVIIVSAIL GIMVFIATIV YIMGVNPTAQ
210 220 230 240 250
ASGSMYGSQI YMICNQFYTP GGTGLYVDQY LYHYCVVDPQ EAIAIVLGFM
260 270 280 290 300
IIVAFALIIF FAVKTRRKMD RYDKSNILWD KEHIYDEQPP NVEEWVKNVS
310 320 330 340 350
AGTQDMPPPP SDYAERVDSP MAYSSNGKVN GKRSYPESFY KSTPLVPEVA
360 370 380 390 400
QEIPLTLSVD DFRQPRYSSN GNLETPSKRA PTKGKAGKGK RTDPDHYETD
410 420 430 440 450
YTTGGESCEE LEEDWVREYP PITSDQQRQL YKRNFDAGLQ EYKSLQAELD
460 470 480 490 500
DVNKELSRLD KELDDYREES EEYMAAADEY NRLKQVKGSA DYKSKRNYCK
510 520
QLKSKLSHIK RMVGDYDRRK P
Length:521
Mass (Da):59,000
Last modified:November 1, 1996 - v1
Checksum:i21D62F370EB33E7D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U49185 mRNA. Translation: AAC52515.1.
AK019880 mRNA. Translation: BAB31900.1.
AK042576 mRNA. Translation: BAC31298.1.
CCDSiCCDS26731.1.
RefSeqiNP_032782.1. NM_008756.2.
XP_006517626.1. XM_006517563.3.
XP_006517627.1. XM_006517564.3.
XP_006517629.1. XM_006517566.2.
XP_011242936.1. XM_011244634.2.
UniGeneiMm.4807.

Genome annotation databases

EnsembliENSMUST00000022140; ENSMUSP00000022140; ENSMUSG00000021638.
ENSMUST00000069756; ENSMUSP00000065284; ENSMUSG00000021638.
ENSMUST00000160859; ENSMUSP00000124849; ENSMUSG00000021638.
GeneIDi18260.
KEGGimmu:18260.
UCSCiuc007rqy.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U49185 mRNA. Translation: AAC52515.1.
AK019880 mRNA. Translation: BAB31900.1.
AK042576 mRNA. Translation: BAC31298.1.
CCDSiCCDS26731.1.
RefSeqiNP_032782.1. NM_008756.2.
XP_006517626.1. XM_006517563.3.
XP_006517627.1. XM_006517564.3.
XP_006517629.1. XM_006517566.2.
XP_011242936.1. XM_011244634.2.
UniGeneiMm.4807.

3D structure databases

ProteinModelPortaliQ61146.
SMRiQ61146.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi201894. 6 interactors.
DIPiDIP-49005N.
STRINGi10090.ENSMUSP00000022140.

PTM databases

iPTMnetiQ61146.
PhosphoSitePlusiQ61146.

Proteomic databases

MaxQBiQ61146.
PaxDbiQ61146.
PRIDEiQ61146.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000022140; ENSMUSP00000022140; ENSMUSG00000021638.
ENSMUST00000069756; ENSMUSP00000065284; ENSMUSG00000021638.
ENSMUST00000160859; ENSMUSP00000124849; ENSMUSG00000021638.
GeneIDi18260.
KEGGimmu:18260.
UCSCiuc007rqy.1. mouse.

Organism-specific databases

CTDi100506658.
MGIiMGI:106183. Ocln.

Phylogenomic databases

eggNOGiENOG410IGK8. Eukaryota.
ENOG4110E0E. LUCA.
GeneTreeiENSGT00730000110989.
HOGENOMiHOG000233490.
HOVERGENiHBG004523.
InParanoidiQ61146.
KOiK06088.
OMAiYAPSNDV.
OrthoDBiEOG091G0BWN.
PhylomeDBiQ61146.
TreeFamiTF326161.

Miscellaneous databases

PROiQ61146.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000021638.
CleanExiMM_OCLN.
ExpressionAtlasiQ61146. baseline and differential.
GenevisibleiQ61146. MM.

Family and domain databases

InterProiIPR031176. ELL/occludin.
IPR008253. Marvel.
IPR002958. Occludin.
IPR010844. Occludin_ELL.
[Graphical view]
PANTHERiPTHR23288. PTHR23288. 1 hit.
PTHR23288:SF4. PTHR23288:SF4. 1 hit.
PfamiPF01284. MARVEL. 1 hit.
PF07303. Occludin_ELL. 1 hit.
[Graphical view]
PIRSFiPIRSF005993. Occludin. 1 hit.
PRINTSiPR01258. OCCLUDIN.
PROSITEiPS51225. MARVEL. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiOCLN_MOUSE
AccessioniPrimary (citable) accession number: Q61146
Secondary accession number(s): Q544A7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: November 1, 1996
Last modified: November 30, 2016
This is version 140 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.