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Q61143 (TRPC6_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 129. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Short transient receptor potential channel 6

Short name=TrpC6
Alternative name(s):
Calcium entry channel
Transient receptor protein 6
Short name=TRP-6
Gene names
Name:Trpc6
Synonyms:Trp6, Trrp6
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length930 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Thought to form a receptor-activated non-selective calcium permeant cation channel. Probably is operated by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases or G-protein coupled receptors. Activated by diacylglycerol (DAG) in a membrane-delimited fashion, independently of protein kinase C. Seems not to be activated by intracellular calcium store depletion.

Subunit structure

Interacts with MX1 and RNF24 By similarity.

Subcellular location

Membrane; Multi-pass membrane protein Probable.

Tissue specificity

Lung and brain.

Post-translational modification

Phosphorylated by FYN, leading to an increase of TRPC6 channel activity. Ref.6

N-glycosylated.

Sequence similarities

Belongs to the transient receptor (TC 1.A.4) family. STrpC subfamily. TRPC6 sub-subfamily. [View classification]

Contains 4 ANK repeats.

Ontologies

Keywords
   Biological processCalcium transport
Ion transport
Transport
   Cellular componentMembrane
   DomainANK repeat
Repeat
Transmembrane
Transmembrane helix
   LigandCalcium
   Molecular functionCalcium channel
Ion channel
   PTMGlycoprotein
Phosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processaging

Inferred from electronic annotation. Source: Ensembl

cellular response to hydrogen peroxide

Inferred from electronic annotation. Source: Ensembl

cellular response to hypoxia

Inferred from electronic annotation. Source: Ensembl

positive regulation of calcium ion transport

Inferred from sequence orthology PubMed 10998353. Source: MGI

positive regulation of cytosolic calcium ion concentration

Inferred from direct assay Ref.1. Source: MGI

positive regulation of ion transmembrane transporter activity

Inferred from sequence orthology PubMed 10998353. Source: MGI

   Cellular_componentintegral component of plasma membrane

Inferred from sequence alignment Ref.1. Source: MGI

plasma membrane

Inferred from direct assay PubMed 15924139. Source: MGI

slit diaphragm

Inferred from direct assay PubMed 15924139. Source: MGI

   Molecular_functioninositol 1,4,5 trisphosphate binding

Inferred from electronic annotation. Source: Ensembl

ion channel activity

Inferred from direct assay PubMed 15194687. Source: MGI

protein binding

Inferred from physical interaction PubMed 15924139. Source: MGI

store-operated calcium channel activity

Inferred from direct assay Ref.5. Source: MGI

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 930930Short transient receptor potential channel 6
PRO_0000215323

Regions

Topological domain1 – 437437Cytoplasmic Potential
Transmembrane438 – 45821Helical; Potential
Topological domain459 – 48628Extracellular Potential
Transmembrane487 – 50721Helical; Potential
Topological domain508 – 52013Cytoplasmic Potential
Transmembrane521 – 54121Helical; Potential
Topological domain542 – 59150Extracellular Potential
Transmembrane592 – 61221Helical; Potential
Topological domain613 – 63523Cytoplasmic Potential
Transmembrane636 – 65621Helical; Potential
Topological domain657 – 70549Extracellular Potential
Transmembrane706 – 72621Helical; Potential
Topological domain727 – 930204Cytoplasmic Potential
Repeat96 – 12530ANK 1
Repeat131 – 16030ANK 2
Repeat162 – 18827ANK 3
Repeat217 – 24630ANK 4

Amino acid modifications

Modified residue8141Phosphoserine By similarity
Glycosylation5601N-linked (GlcNAc...) Potential

Experimental info

Sequence conflict3 – 5654Missing Ref.2
Sequence conflict1051A → V in AAC06146. Ref.1
Sequence conflict1141R → W in AAC06146. Ref.1
Sequence conflict1341N → D in AAC64394. Ref.2
Sequence conflict1841A → S in AAC06146. Ref.1
Sequence conflict3711Y → D in AAC06146. Ref.1
Sequence conflict436 – 4372RG → PR in AAC06146. Ref.1
Sequence conflict9051T → S in AAC06146. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q61143 [UniParc].

Last modified July 27, 2011. Version 3.
Checksum: 9501C8D50FF33430

FASTA930106,681
        10         20         30         40         50         60 
MSQSPRFVTR RGGSLKAAPG AGTRRNESQD YLLMDELGDD GYPQLPLPPY GYYPSFRGNE 

        70         80         90        100        110        120 
NRLTHRRQTI LREKGRRLAN RGPAYMFNDH STSLSIEEER FLDAAEYGNI PVVRKMLEEC 

       130        140        150        160        170        180 
HSLNVNCVDY MGQNALQLAV ANEHLEITEL LLKKENLSRV GDALLLAISK GYVRIVEAIL 

       190        200        210        220        230        240 
NHPAFAEGKR LATSPSQSEL QQDDFYAYDE DGTRFSHDVT PIILAAHCQE YEIVHTLLRK 

       250        260        270        280        290        300 
GARIERPHDY FCKCTECSQK QKHDSFSHSR SRINAYKGLA SPAYLSLSSE DPVMTALELS 

       310        320        330        340        350        360 
NELAVLANIE KEFKNDYRKL SMQCKDFVVG LLDLCRNTEE VEAILNGDAE TRQPGDFGRP 

       370        380        390        400        410        420 
NLSRLKLAIK YEVKKFVAHP NCQQQLLSIW YENLSGLRQQ TMAVKFLVVL AVAIGLPFLA 

       430        440        450        460        470        480 
LIYWCAPCSK MGKILRGPFM KFVAHAASFT IFLGLLVMNA ADRFEGTKLL PNETSTDNAR 

       490        500        510        520        530        540 
QLFRMKTSCF SWMEMLIISW VIGMIWAECK EIWTQGPKEY LFELWNMLDF GMLAIFAASF 

       550        560        570        580        590        600 
IARFMAFWHA SKAQSIIDAN DTLKDLTKVT LGDNVKYYNL ARIKWDPTDP QIISEGLYAI 

       610        620        630        640        650        660 
AVVLSFSRIA YILPANESFG PLQISLGRTV KDIFKFMVIF IMVFVAFMIG MFNLYSYYIG 

       670        680        690        700        710        720 
AKQNEAFTTV EESFKTLFWA IFGLSEVKSV VINYNHKFIE NIGYVLYGVY NVTMVIVLLN 

       730        740        750        760        770        780 
MLIAMINSSF QEIEDDADVE WKFARAKLWF SYFEEGRTLP VPFNLVPSPK SLLYLLLKFK 

       790        800        810        820        830        840 
KWMCELIQGQ KQGFQEDAEM NKRNEEKKFG ISGSHEDLSK FSLDKNQLAH NKQSSTRSSE 

       850        860        870        880        890        900 
DYHLNSFSNP PRQYQKIMKR LIKRYVLQAQ IDKESDEVNE GELKEIKQDI SSLRYELLEE 

       910        920        930 
KSQNTEDLAE LIRKLGERLS LEPKLEESRR 

« Hide

References

« Hide 'large scale' references
[1]"Cloning and expression of a novel mammalian homolog of Drosophila transient receptor potential (Trp) involved in calcium entry secondary to activation of receptors coupled by the Gq class of G protein."
Boulay G., Zhu X., Peyton M., Jiang M., Hurst R., Stefani E., Birnbaumer L.
J. Biol. Chem. 272:29672-29680(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Brain.
[2]"Search for oncogenic regulators in an autocrine tumor model using differential display PCR: identification of novel candidate genes including the calcium channel mtrp6."
Buess M., Engler O., Hirsch H.H., Moroni C.
Oncogene 18:1487-1494(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: DBA/2.
[3]Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Embryo.
[5]"trp, a novel mammalian gene family essential for agonist-activated capacitative Ca2+ entry."
Zhu X., Jiang M., Peyton M., Boulay G., Hurst R., Stefani E., Birnbaumer L.
Cell 85:661-671(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 631-739.
Tissue: Brain.
[6]"Regulation of TRPC6 channel activity by tyrosine phosphorylation."
Hisatsune C., Kuroda Y., Nakamura K., Inoue T., Nakamura T., Michikawa T., Mizutani A., Mikoshiba K.
J. Biol. Chem. 279:18887-18894(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION BY FYN.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U49069 mRNA. Translation: AAC06146.1.
AF057748 mRNA. Translation: AAC64394.1.
CH466522 Genomic DNA. Translation: EDL24958.1.
BC141131 mRNA. Translation: AAI41132.1.
CCDSCCDS22815.1.
RefSeqNP_038866.2. NM_013838.2.
XP_006509913.1. XM_006509850.1.
UniGeneMm.325086.

3D structure databases

ProteinModelPortalQ61143.
SMRQ61143. Positions 99-253.
ModBaseSearch...
MobiDBSearch...

Chemistry

ChEMBLCHEMBL1795081.
GuidetoPHARMACOLOGY491.

PTM databases

PhosphoSiteQ61143.

Proteomic databases

PaxDbQ61143.
PRIDEQ61143.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000050433; ENSMUSP00000057965; ENSMUSG00000031997.
GeneID22068.
KEGGmmu:22068.
UCSCuc009odl.1. mouse.

Organism-specific databases

CTD7225.
MGIMGI:109523. Trpc6.

Phylogenomic databases

eggNOGNOG254238.
GeneTreeENSGT00680000099764.
HOGENOMHOG000020590.
HOVERGENHBG068337.
InParanoidQ61143.
KOK04969.
OMANRGPAYM.
OrthoDBEOG77HDD1.
TreeFamTF313147.

Gene expression databases

BgeeQ61143.
GenevestigatorQ61143.

Family and domain databases

Gene3D1.25.40.20. 1 hit.
InterProIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR005821. Ion_trans_dom.
IPR004729. TRP_channel.
IPR013555. TRP_dom.
IPR005462. TRPC6_channel.
IPR002153. TRPC_channel.
[Graphical view]
PANTHERPTHR10117. PTHR10117. 1 hit.
PTHR10117:SF7. PTHR10117:SF7. 1 hit.
PfamPF12796. Ank_2. 1 hit.
PF00520. Ion_trans. 1 hit.
PF08344. TRP_2. 1 hit.
[Graphical view]
PRINTSPR01097. TRNSRECEPTRP.
PR01647. TRPCHANNEL6.
SMARTSM00248. ANK. 3 hits.
[Graphical view]
SUPFAMSSF48403. SSF48403. 1 hit.
TIGRFAMsTIGR00870. trp. 1 hit.
PROSITEPS50297. ANK_REP_REGION. 2 hits.
PS50088. ANK_REPEAT. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio301892.
PROQ61143.
SOURCESearch...

Entry information

Entry nameTRPC6_MOUSE
AccessionPrimary (citable) accession number: Q61143
Secondary accession number(s): B9EIW2, Q9Z2J1
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: July 27, 2011
Last modified: July 9, 2014
This is version 129 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot