UniProtKB - Q61140 (BCAR1_MOUSE)
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Protein
Breast cancer anti-estrogen resistance protein 1
Gene
Bcar1
Organism
Mus musculus (Mouse)
Status
Functioni
Docking protein which plays a central coordinating role for tyrosine kinase-based signaling related to cell adhesion. Implicated in induction of cell migration (By similarity). Has been shown to be essential in cardiovascular development during embryogenesis.By similarity
GO - Molecular functioni
- protein kinase binding Source: MGI
- SH3 domain binding Source: UniProtKB-KW
- signal transducer activity Source: InterPro
GO - Biological processi
- actin filament organization Source: UniProtKB
- antigen receptor-mediated signaling pathway Source: UniProtKB
- B cell receptor signaling pathway Source: UniProtKB
- cell adhesion Source: UniProtKB-KW
- cell chemotaxis Source: MGI
- cell migration Source: MGI
- cellular response to hepatocyte growth factor stimulus Source: MGI
- epidermal growth factor receptor signaling pathway Source: UniProtKB
- G-protein coupled receptor signaling pathway Source: UniProtKB
- hepatocyte growth factor receptor signaling pathway Source: MGI
- insulin receptor signaling pathway Source: UniProtKB
- integrin-mediated signaling pathway Source: UniProtKB
- neurotrophin TRK receptor signaling pathway Source: UniProtKB
- platelet-derived growth factor receptor signaling pathway Source: UniProtKB
- positive regulation of cell migration Source: UniProtKB
- positive regulation of endothelial cell migration Source: MGI
- vascular endothelial growth factor receptor signaling pathway Source: MGI
Keywordsi
Biological process | Cell adhesion |
Enzyme and pathway databases
Reactomei | R-MMU-186763. Downstream signal transduction. R-MMU-372708. p130Cas linkage to MAPK signaling for integrins. R-MMU-4420097. VEGFA-VEGFR2 Pathway. R-MMU-8849471. PTK6 Regulates RHO GTPases, RAS GTPase and MAP kinases. |
Names & Taxonomyi
Protein namesi | Recommended name: Breast cancer anti-estrogen resistance protein 1Alternative name(s): CRK-associated substrate p130cas |
Gene namesi | Name:Bcar1 Synonyms:Cas, Crkas |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:108091. Bcar1. |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000064855 | 1 – 874 | Breast cancer anti-estrogen resistance protein 1Add BLAST | 874 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 1 | N-acetylmethionineBy similarity | 1 | |
Modified residuei | 132 | PhosphotyrosineCombined sources | 1 | |
Modified residuei | 143 | PhosphoserineBy similarity | 1 | |
Modified residuei | 238 | PhosphotyrosineCombined sources | 1 | |
Modified residuei | 253 | PhosphotyrosineCombined sources | 1 | |
Modified residuei | 273 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 296 | PhosphoserineBy similarity | 1 | |
Modified residuei | 366 | PhosphotyrosineCombined sources | 1 | |
Modified residuei | 376 | PhosphotyrosineCombined sources | 1 | |
Modified residuei | 414 | PhosphotyrosineCombined sources | 1 | |
Modified residuei | 432 | PhosphoserineBy similarity | 1 | |
Modified residuei | 441 | PhosphoserineBy similarity | 1 | |
Modified residuei | 643 | PhosphoserineBy similarity | 1 |
Post-translational modificationi
PTK2/FAK1 activation mediates phosphorylation at the YDYVHL motif; phosphorylation is most likely catalyzed by SRC family members. SRC-family kinases are recruited to the phosphorylated sites and can phosphorylate other tyrosine residues. Tyrosine phosphorylation is triggered by integrin mediated adhesion of cells to the extracellular matrix (By similarity).By similarity
Dephosphorylated by PTPN14 at Tyr-132.By similarity
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
PaxDbi | Q61140. |
PRIDEi | Q61140. |
PTM databases
iPTMneti | Q61140. |
PhosphoSitePlusi | Q61140. |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000031955. |
CleanExi | MM_BCAR1. |
Genevisiblei | Q61140. MM. |
Interactioni
Subunit structurei
Forms complexes in vivo with PTK2/FAK1, adapter protein CRKL and LYN kinase. Can heterodimerize with NEDD9. Interacts with activated CSPG4. Interacts with BMX, INPPL1/SHIP2 and PEAK1 (By similarity). Part of a collagen stimulated complex involved in cell migration composed of CDC42, CRK, TNK2 and BCAR1/p130cas (By similarity). Interacts with BCAR3, the interaction regulates adhesion-dependent serine phosphorylation. Interacts with NPHP1, PTK2B/PYK2 and SH2D3C.By similarity6 Publications
Binary interactionsi
GO - Molecular functioni
- protein kinase binding Source: MGI
- SH3 domain binding Source: UniProtKB-KW
Protein-protein interaction databases
BioGridi | 198886. 8 interactors. |
CORUMi | Q61140. |
IntActi | Q61140. 15 interactors. |
MINTi | Q61140. |
STRINGi | 10090.ENSMUSP00000129584. |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more detailsFeature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Helixi | 742 – 774 | Combined sources | 33 | |
Helixi | 779 – 806 | Combined sources | 28 | |
Helixi | 810 – 839 | Combined sources | 30 | |
Helixi | 844 – 872 | Combined sources | 29 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
5W93 | X-ray | 2.00 | A/B/C | 738-874 | [»] | |
ProteinModelPortali | Q61140. | |||||
SMRi | Q61140. | |||||
ModBasei | Search... | |||||
MobiDBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 3 – 65 | SH3PROSITE-ProRule annotationAdd BLAST | 63 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 119 – 420 | Substrate for kinasesBy similarityAdd BLAST | 302 | |
Regioni | 750 – 800 | Divergent helix-loop-helix motifAdd BLAST | 51 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 639 – 647 | SH3-bindingSequence analysis | 9 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 74 – 87 | Pro-richAdd BLAST | 14 | |
Compositional biasi | 426 – 618 | Ser-richAdd BLAST | 193 |
Domaini
Contains a central domain (substrate domain) containing multiple potential SH2-binding sites and a C-terminal domain containing a divergent helix-loop-helix (HLH) motif. The SH2-binding sites putatively bind CRK, NCK and ABL SH2 domains. The HLH motif is absolutely required for the induction of pseudohyphal growth in yeast and mediates heterodimerization with NEDD9.
A serine-rich region promotes activation of the serum response element (SRE).By similarity
The SH3 domain is necessary for the localization of the protein to focal adhesions and interacts with one proline-rich region of PTK2/FAK1.
Sequence similaritiesi
Belongs to the CAS family.Curated
Keywords - Domaini
SH3 domain, SH3-bindingPhylogenomic databases
eggNOGi | ENOG410IEDD. Eukaryota. ENOG410ZSUM. LUCA. |
GeneTreei | ENSGT00490000043324. |
HOGENOMi | HOG000261698. |
HOVERGENi | HBG004354. |
InParanoidi | Q61140. |
KOi | K05726. |
OMAi | TVEHNQP. |
OrthoDBi | EOG091G0RVA. |
TreeFami | TF328782. |
Family and domain databases
CDDi | cd12001. SH3_BCAR1. 1 hit. |
Gene3Di | 1.20.120.830. 1 hit. |
InterProi | View protein in InterPro IPR028848. BCAR1. IPR035745. BCAR1_SH3. IPR021901. CAS_C. IPR037362. CAS_fam. IPR014928. Serine_rich_dom. IPR038319. Serine_rich_sf. IPR036028. SH3-like_dom_sf. IPR001452. SH3_domain. |
PANTHERi | PTHR10654. PTHR10654. 1 hit. PTHR10654:SF15. PTHR10654:SF15. 1 hit. |
Pfami | View protein in Pfam PF12026. DUF3513. 1 hit. PF08824. Serine_rich. 1 hit. PF00018. SH3_1. 1 hit. |
PRINTSi | PR00452. SH3DOMAIN. |
SMARTi | View protein in SMART SM00326. SH3. 1 hit. |
SUPFAMi | SSF50044. SSF50044. 1 hit. |
PROSITEi | View protein in PROSITE PS50002. SH3. 1 hit. |
s (2)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basket
Isoform Cas-B (identifier: Q61140-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
10 20 30 40 50
MTVPNVLAKA LYDNVAESPD ELSFRKGDIM TVLERDTQGL DGWWLCSLHG
60 70 80 90 100
RQGIVPGNRL KILVGMYDKK PVGPGPGPPA TPPQPQPSLP QGVHAPVPPA
110 120 130 140 150
SQYSPMLPTA YQPQSDNVYL VPTPSKTQQG LYQAPGPNPQ FQSPPAKQTS
160 170 180 190 200
TFSKQTPHHS FPSPATDLYQ VPPGPGSPAQ DIYQVPPSAG IGHDIYQVPP
210 220 230 240 250
SLDTRGWEGT KPPAKVVVPT RVGQGYVYEA AQTEQDEYDT PRHLLAPGPQ
260 270 280 290 300
DIYDVPPVRG LLPNQYGQEV YDTPPMAVKG PNGRDPLLDV YDVPPSVEKG
310 320 330 340 350
LLSSSHHSVY DVPPSVSKDV PDGPLLREET YDVPPAFAKP KPFDPTRHPL
360 370 380 390 400
ILAAPPPDSP AAEDVYDVPP PAPDLYDVPP GLRRPGPGTL YDVPRERVLP
410 420 430 440 450
PEVADGSVVD DGVYAVPPPA EREAPTDGKR LSASSTGSTR SSQSASSLEV
460 470 480 490 500
VVPGREPLEL EVAVESLARL QQGVSTTVAH LLDLVGSASG PGGWRGTSEP
510 520 530 540 550
QEPPAQDLKA AVAAVHGAVH ELLEFARGAV SNATHTSDRT LHAKLSRQLQ
560 570 580 590 600
KMEDVYQTLV VHGQVLDSGR GSPGFTPEDL DRLVACSRAV PEDAKQLASF
610 620 630 640 650
LHGNASLLFR RTKAPGPGPE GSSSLHPNPT DKASSIQSRP LPSPPKFTSQ
660 670 680 690 700
DSPDGQYENS EGGWMEDYDY VHLQGKEEFE KTQKELLERG NIMRQGKGQL
710 720 730 740 750
ELQQLKQFER LEQEVSRPID HDLANWTPAQ PLVPGRTGGL GPSDRQLLLF
760 770 780 790 800
YLEQCEANLT TLTDAVDAFF TAVATNQPPK IFVAHSKFVI LSAHKLVFIG
810 820 830 840 850
DTLSRQAKAA DVRSQVTHYS NLLCDLLRGI VATTKAAALQ YPSPSAAQDM
860 870
VDRVKELGHS TQQFRRVLGQ LAAA
Isoform Cas-A (identifier: Q61140-2) [UniParc]FASTAAdd to basket
The sequence of this isoform differs from the canonical sequence as follows:
1-4: MTVP → MKYL
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 72 | V → A in AAA93381 (PubMed:7479864).Curated | 1 | |
Sequence conflicti | 72 | V → A in AAA93248 (PubMed:7479864).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_004134 | 1 – 4 | MTVP → MKYL in isoform Cas-A. 1 Publication | 4 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U48853 mRNA. Translation: AAA93381.1. U28151 mRNA. Translation: AAA93248.1. AK145863 mRNA. Translation: BAE26705.1. BC057578 mRNA. Translation: AAH57578.1. |
CCDSi | CCDS52675.1. [Q61140-1] CCDS85616.1. [Q61140-2] |
RefSeqi | NP_001185768.1. NM_001198839.1. [Q61140-2] NP_034084.2. NM_009954.3. [Q61140-1] |
UniGenei | Mm.3758. |
Genome annotation databases
Ensembli | ENSMUST00000166232; ENSMUSP00000129584; ENSMUSG00000031955. [Q61140-1] ENSMUST00000212349; ENSMUSP00000148364; ENSMUSG00000031955. [Q61140-2] |
GeneIDi | 12927. |
KEGGi | mmu:12927. |
UCSCi | uc009nmt.2. mouse. [Q61140-2] uc009nmu.2. mouse. [Q61140-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Entry informationi
Entry namei | BCAR1_MOUSE | |
Accessioni | Q61140Primary (citable) accession number: Q61140 Secondary accession number(s): Q60869, Q6PFF9 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 30, 2000 |
Last sequence update: | July 27, 2011 | |
Last modified: | March 28, 2018 | |
This is version 169 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |