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Protein

Breast cancer anti-estrogen resistance protein 1

Gene

Bcar1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Docking protein which plays a central coordinating role for tyrosine kinase-based signaling related to cell adhesion. Implicated in induction of cell migration (By similarity). Has been shown to be essential in cardiovascular development during embryogenesis.By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Cell adhesion

Enzyme and pathway databases

ReactomeiR-MMU-186763. Downstream signal transduction.
R-MMU-372708. p130Cas linkage to MAPK signaling for integrins.
R-MMU-4420097. VEGFA-VEGFR2 Pathway.
R-MMU-8849471. PTK6 Regulates RHO GTPases, RAS GTPase and MAP kinases.

Names & Taxonomyi

Protein namesi
Recommended name:
Breast cancer anti-estrogen resistance protein 1
Alternative name(s):
CRK-associated substrate
p130cas
Gene namesi
Name:Bcar1
Synonyms:Cas, Crkas
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 8

Organism-specific databases

MGIiMGI:108091. Bcar1.

Subcellular locationi

  • Cell junctionfocal adhesion By similarity
  • Cytoplasm By similarity

  • Note: Unphosphorylated form localizes in the cytoplasm and can move to the membrane upon tyrosine phosphorylation.By similarity

GO - Cellular componenti

  • actin cytoskeleton Source: MGI
  • cytoplasm Source: UniProtKB
  • cytosol Source: Reactome
  • focal adhesion Source: UniProtKB
  • lamellipodium Source: MGI
  • nucleolus Source: MGI
  • plasma membrane Source: MGI
  • ruffle Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000648551 – 874Breast cancer anti-estrogen resistance protein 1Add BLAST874

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei132PhosphotyrosineCombined sources1
Modified residuei143PhosphoserineBy similarity1
Modified residuei238PhosphotyrosineCombined sources1
Modified residuei253PhosphotyrosineCombined sources1
Modified residuei273PhosphothreonineBy similarity1
Modified residuei296PhosphoserineBy similarity1
Modified residuei366PhosphotyrosineCombined sources1
Modified residuei376PhosphotyrosineCombined sources1
Modified residuei414PhosphotyrosineCombined sources1
Modified residuei432PhosphoserineBy similarity1
Modified residuei441PhosphoserineBy similarity1
Modified residuei643PhosphoserineBy similarity1

Post-translational modificationi

PTK2/FAK1 activation mediates phosphorylation at the YDYVHL motif; phosphorylation is most likely catalyzed by SRC family members. SRC-family kinases are recruited to the phosphorylated sites and can phosphorylate other tyrosine residues. Tyrosine phosphorylation is triggered by integrin mediated adhesion of cells to the extracellular matrix (By similarity).By similarity
Dephosphorylated by PTPN14 at Tyr-132.By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ61140.
PRIDEiQ61140.

PTM databases

iPTMnetiQ61140.
PhosphoSitePlusiQ61140.

Expressioni

Gene expression databases

BgeeiENSMUSG00000031955.
CleanExiMM_BCAR1.
GenevisibleiQ61140. MM.

Interactioni

Subunit structurei

Forms complexes in vivo with PTK2/FAK1, adapter protein CRKL and LYN kinase. Can heterodimerize with NEDD9. Interacts with activated CSPG4. Interacts with BMX, INPPL1/SHIP2 and PEAK1 (By similarity). Part of a collagen stimulated complex involved in cell migration composed of CDC42, CRK, TNK2 and BCAR1/p130cas (By similarity). Interacts with BCAR3, the interaction regulates adhesion-dependent serine phosphorylation. Interacts with NPHP1, PTK2B/PYK2 and SH2D3C.By similarity6 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Nphp1Q9QY532EBI-77088,EBI-77230
Ptk2P341523EBI-77088,EBI-77070
PTPN1P180315EBI-77088,EBI-968788From a different organism.
Srcin1Q9QWI62EBI-77088,EBI-775592

GO - Molecular functioni

Protein-protein interaction databases

BioGridi198886. 7 interactors.
IntActiQ61140. 14 interactors.
MINTiMINT-100691.
STRINGi10090.ENSMUSP00000129584.

Structurei

3D structure databases

ProteinModelPortaliQ61140.
SMRiQ61140.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini6 – 26SH3PROSITE-ProRule annotationAdd BLAST21

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni119 – 420Substrate for kinasesBy similarityAdd BLAST302
Regioni750 – 800Divergent helix-loop-helix motifAdd BLAST51

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi639 – 647SH3-bindingSequence analysis9

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi74 – 87Pro-richAdd BLAST14
Compositional biasi426 – 618Ser-richAdd BLAST193

Domaini

Contains a central domain (substrate domain) containing multiple potential SH2-binding sites and a C-terminal domain containing a divergent helix-loop-helix (HLH) motif. The SH2-binding sites putatively bind CRK, NCK and ABL SH2 domains. The HLH motif is absolutely required for the induction of pseudohyphal growth in yeast and mediates heterodimerization with NEDD9.
A serine-rich region promotes activation of the serum response element (SRE).By similarity
The SH3 domain is necessary for the localization of the protein to focal adhesions and interacts with one proline-rich region of PTK2/FAK1.

Sequence similaritiesi

Belongs to the CAS family.Curated
Contains 1 SH3 domain.PROSITE-ProRule annotation

Keywords - Domaini

SH3 domain, SH3-binding

Phylogenomic databases

eggNOGiENOG410IEDD. Eukaryota.
ENOG410ZSUM. LUCA.
GeneTreeiENSGT00490000043324.
HOGENOMiHOG000261698.
HOVERGENiHBG004354.
InParanoidiQ61140.
KOiK05726.
OMAiAKAKPFD.
OrthoDBiEOG091G0RVA.
TreeFamiTF328782.

Family and domain databases

InterProiIPR028848. BCAR1.
IPR021901. CAS_DUF3513.
IPR014928. Serine_rich.
IPR001452. SH3_domain.
IPR013315. Spectrin_alpha_SH3.
[Graphical view]
PANTHERiPTHR10654:SF15. PTHR10654:SF15. 2 hits.
PfamiPF12026. DUF3513. 1 hit.
PF08824. Serine_rich. 1 hit.
PF00018. SH3_1. 1 hit.
[Graphical view]
PRINTSiPR00452. SH3DOMAIN.
PR01887. SPECTRNALPHA.
SMARTiSM00326. SH3. 1 hit.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 1 hit.
PROSITEiPS50002. SH3. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform Cas-B (identifier: Q61140-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MTVPNVLAKA LYDNVAESPD ELSFRKGDIM TVLERDTQGL DGWWLCSLHG
60 70 80 90 100
RQGIVPGNRL KILVGMYDKK PVGPGPGPPA TPPQPQPSLP QGVHAPVPPA
110 120 130 140 150
SQYSPMLPTA YQPQSDNVYL VPTPSKTQQG LYQAPGPNPQ FQSPPAKQTS
160 170 180 190 200
TFSKQTPHHS FPSPATDLYQ VPPGPGSPAQ DIYQVPPSAG IGHDIYQVPP
210 220 230 240 250
SLDTRGWEGT KPPAKVVVPT RVGQGYVYEA AQTEQDEYDT PRHLLAPGPQ
260 270 280 290 300
DIYDVPPVRG LLPNQYGQEV YDTPPMAVKG PNGRDPLLDV YDVPPSVEKG
310 320 330 340 350
LLSSSHHSVY DVPPSVSKDV PDGPLLREET YDVPPAFAKP KPFDPTRHPL
360 370 380 390 400
ILAAPPPDSP AAEDVYDVPP PAPDLYDVPP GLRRPGPGTL YDVPRERVLP
410 420 430 440 450
PEVADGSVVD DGVYAVPPPA EREAPTDGKR LSASSTGSTR SSQSASSLEV
460 470 480 490 500
VVPGREPLEL EVAVESLARL QQGVSTTVAH LLDLVGSASG PGGWRGTSEP
510 520 530 540 550
QEPPAQDLKA AVAAVHGAVH ELLEFARGAV SNATHTSDRT LHAKLSRQLQ
560 570 580 590 600
KMEDVYQTLV VHGQVLDSGR GSPGFTPEDL DRLVACSRAV PEDAKQLASF
610 620 630 640 650
LHGNASLLFR RTKAPGPGPE GSSSLHPNPT DKASSIQSRP LPSPPKFTSQ
660 670 680 690 700
DSPDGQYENS EGGWMEDYDY VHLQGKEEFE KTQKELLERG NIMRQGKGQL
710 720 730 740 750
ELQQLKQFER LEQEVSRPID HDLANWTPAQ PLVPGRTGGL GPSDRQLLLF
760 770 780 790 800
YLEQCEANLT TLTDAVDAFF TAVATNQPPK IFVAHSKFVI LSAHKLVFIG
810 820 830 840 850
DTLSRQAKAA DVRSQVTHYS NLLCDLLRGI VATTKAAALQ YPSPSAAQDM
860 870
VDRVKELGHS TQQFRRVLGQ LAAA
Length:874
Mass (Da):94,285
Last modified:July 27, 2011 - v2
Checksum:i81E56BC7AD87B095
GO
Isoform Cas-A (identifier: Q61140-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-4: MTVP → MKYL

Show »
Length:874
Mass (Da):94,392
Checksum:i25B8F5D1D61CC8B1
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti72V → A in AAA93381 (PubMed:7479864).Curated1
Sequence conflicti72V → A in AAA93248 (PubMed:7479864).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0041341 – 4MTVP → MKYL in isoform Cas-A. 1 Publication4

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U48853 mRNA. Translation: AAA93381.1.
U28151 mRNA. Translation: AAA93248.1.
AK145863 mRNA. Translation: BAE26705.1.
BC057578 mRNA. Translation: AAH57578.1.
CCDSiCCDS52675.1. [Q61140-1]
RefSeqiNP_001185768.1. NM_001198839.1. [Q61140-2]
NP_034084.2. NM_009954.3. [Q61140-1]
UniGeneiMm.3758.

Genome annotation databases

EnsembliENSMUST00000166232; ENSMUSP00000129584; ENSMUSG00000031955. [Q61140-1]
ENSMUST00000212349; ENSMUSP00000148364; ENSMUSG00000031955. [Q61140-2]
GeneIDi12927.
KEGGimmu:12927.
UCSCiuc009nmt.2. mouse. [Q61140-2]
uc009nmu.2. mouse. [Q61140-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U48853 mRNA. Translation: AAA93381.1.
U28151 mRNA. Translation: AAA93248.1.
AK145863 mRNA. Translation: BAE26705.1.
BC057578 mRNA. Translation: AAH57578.1.
CCDSiCCDS52675.1. [Q61140-1]
RefSeqiNP_001185768.1. NM_001198839.1. [Q61140-2]
NP_034084.2. NM_009954.3. [Q61140-1]
UniGeneiMm.3758.

3D structure databases

ProteinModelPortaliQ61140.
SMRiQ61140.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi198886. 7 interactors.
IntActiQ61140. 14 interactors.
MINTiMINT-100691.
STRINGi10090.ENSMUSP00000129584.

PTM databases

iPTMnetiQ61140.
PhosphoSitePlusiQ61140.

Proteomic databases

PaxDbiQ61140.
PRIDEiQ61140.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000166232; ENSMUSP00000129584; ENSMUSG00000031955. [Q61140-1]
ENSMUST00000212349; ENSMUSP00000148364; ENSMUSG00000031955. [Q61140-2]
GeneIDi12927.
KEGGimmu:12927.
UCSCiuc009nmt.2. mouse. [Q61140-2]
uc009nmu.2. mouse. [Q61140-1]

Organism-specific databases

CTDi9564.
MGIiMGI:108091. Bcar1.

Phylogenomic databases

eggNOGiENOG410IEDD. Eukaryota.
ENOG410ZSUM. LUCA.
GeneTreeiENSGT00490000043324.
HOGENOMiHOG000261698.
HOVERGENiHBG004354.
InParanoidiQ61140.
KOiK05726.
OMAiAKAKPFD.
OrthoDBiEOG091G0RVA.
TreeFamiTF328782.

Enzyme and pathway databases

ReactomeiR-MMU-186763. Downstream signal transduction.
R-MMU-372708. p130Cas linkage to MAPK signaling for integrins.
R-MMU-4420097. VEGFA-VEGFR2 Pathway.
R-MMU-8849471. PTK6 Regulates RHO GTPases, RAS GTPase and MAP kinases.

Miscellaneous databases

PROiQ61140.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000031955.
CleanExiMM_BCAR1.
GenevisibleiQ61140. MM.

Family and domain databases

InterProiIPR028848. BCAR1.
IPR021901. CAS_DUF3513.
IPR014928. Serine_rich.
IPR001452. SH3_domain.
IPR013315. Spectrin_alpha_SH3.
[Graphical view]
PANTHERiPTHR10654:SF15. PTHR10654:SF15. 2 hits.
PfamiPF12026. DUF3513. 1 hit.
PF08824. Serine_rich. 1 hit.
PF00018. SH3_1. 1 hit.
[Graphical view]
PRINTSiPR00452. SH3DOMAIN.
PR01887. SPECTRNALPHA.
SMARTiSM00326. SH3. 1 hit.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 1 hit.
PROSITEiPS50002. SH3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiBCAR1_MOUSE
AccessioniPrimary (citable) accession number: Q61140
Secondary accession number(s): Q60869, Q6PFF9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: July 27, 2011
Last modified: November 30, 2016
This is version 156 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.