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UniProtKB - Q61140 (BCAR1_MOUSE)

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Protein

Breast cancer anti-estrogen resistance protein 1

Gene

Bcar1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Docking protein which plays a central coordinating role for tyrosine kinase-based signaling related to cell adhesion. Implicated in induction of cell migration (By similarity). Has been shown to be essential in cardiovascular development during embryogenesis.By similarity

GO - Molecular functioni

View the complete GO annotation on QuickGO ...

GO - Biological processi

View the complete GO annotation on QuickGO ...

Keywordsi

Biological processCell adhesion

Enzyme and pathway databases

ReactomeiR-MMU-186763 Downstream signal transduction
R-MMU-372708 p130Cas linkage to MAPK signaling for integrins
R-MMU-4420097 VEGFA-VEGFR2 Pathway
R-MMU-8849471 PTK6 Regulates RHO GTPases, RAS GTPase and MAP kinases

Names & Taxonomyi

Protein namesi
Recommended name:
Breast cancer anti-estrogen resistance protein 1
Alternative name(s):
CRK-associated substrate
p130cas
Gene namesi
Name:Bcar1
Synonyms:Cas, Crkas
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus
Proteomesi
  • UP000000589 Componenti: Chromosome 8

Organism-specific databases

MGIiMGI:108091 Bcar1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell junction, Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000648551 – 874Breast cancer anti-estrogen resistance protein 1Add BLAST874

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei132PhosphotyrosineCombined sources1
Modified residuei143PhosphoserineBy similarity1
Modified residuei238PhosphotyrosineCombined sources1
Modified residuei253PhosphotyrosineCombined sources1
Modified residuei273PhosphothreonineBy similarity1
Modified residuei296PhosphoserineBy similarity1
Modified residuei366PhosphotyrosineCombined sources1
Modified residuei376PhosphotyrosineCombined sources1
Modified residuei414PhosphotyrosineCombined sources1
Modified residuei432PhosphoserineBy similarity1
Modified residuei441PhosphoserineBy similarity1
Modified residuei643PhosphoserineBy similarity1

Post-translational modificationi

PTK2/FAK1 activation mediates phosphorylation at the YDYVHL motif; phosphorylation is most likely catalyzed by SRC family members. SRC-family kinases are recruited to the phosphorylated sites and can phosphorylate other tyrosine residues. Tyrosine phosphorylation is triggered by integrin mediated adhesion of cells to the extracellular matrix (By similarity).By similarity
Dephosphorylated by PTPN14 at Tyr-132.By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ61140
PRIDEiQ61140

PTM databases

iPTMnetiQ61140
PhosphoSitePlusiQ61140

Expressioni

Gene expression databases

BgeeiENSMUSG00000031955
CleanExiMM_BCAR1
GenevisibleiQ61140 MM

Interactioni

Subunit structurei

Forms complexes in vivo with PTK2/FAK1, adapter protein CRKL and LYN kinase. Can heterodimerize with NEDD9. Interacts with activated CSPG4. Interacts with BMX, INPPL1/SHIP2 and PEAK1 (By similarity). Part of a collagen stimulated complex involved in cell migration composed of CDC42, CRK, TNK2 and BCAR1/p130cas (By similarity). Interacts with BCAR3, the interaction regulates adhesion-dependent serine phosphorylation. Interacts with NPHP1, PTK2B/PYK2 and SH2D3C.By similarity6 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

View the complete GO annotation on QuickGO ...

Protein-protein interaction databases

BioGridi198886, 8 interactors
CORUMiQ61140
IntActiQ61140, 14 interactors
MINTiQ61140
STRINGi10090.ENSMUSP00000129584

Structurei

Secondary structure

1874
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi742 – 774Combined sources33
Helixi779 – 806Combined sources28
Helixi810 – 839Combined sources30
Helixi844 – 872Combined sources29

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5W93X-ray2.00A/B/C738-874[»]
ProteinModelPortaliQ61140
SMRiQ61140
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini3 – 65SH3PROSITE-ProRule annotationAdd BLAST63

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni119 – 420Substrate for kinasesBy similarityAdd BLAST302
Regioni750 – 800Divergent helix-loop-helix motifAdd BLAST51

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi639 – 647SH3-bindingSequence analysis9

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi74 – 87Pro-richAdd BLAST14
Compositional biasi426 – 618Ser-richAdd BLAST193

Domaini

Contains a central domain (substrate domain) containing multiple potential SH2-binding sites and a C-terminal domain containing a divergent helix-loop-helix (HLH) motif. The SH2-binding sites putatively bind CRK, NCK and ABL SH2 domains. The HLH motif is absolutely required for the induction of pseudohyphal growth in yeast and mediates heterodimerization with NEDD9.
A serine-rich region promotes activation of the serum response element (SRE).By similarity
The SH3 domain is necessary for the localization of the protein to focal adhesions and interacts with one proline-rich region of PTK2/FAK1.

Sequence similaritiesi

Belongs to the CAS family.Curated

Keywords - Domaini

SH3 domain, SH3-binding

Phylogenomic databases

eggNOGiENOG410IEDD Eukaryota
ENOG410ZSUM LUCA
GeneTreeiENSGT00490000043324
HOGENOMiHOG000261698
HOVERGENiHBG004354
InParanoidiQ61140
KOiK05726
OMAiTVEHNQP
OrthoDBiEOG091G0RVA
TreeFamiTF328782

Family and domain databases

CDDicd12001 SH3_BCAR1, 1 hit
Gene3Di1.20.120.830, 1 hit
InterProiView protein in InterPro
IPR028848 BCAR1
IPR035745 BCAR1_SH3
IPR021901 CAS_C
IPR037362 CAS_fam
IPR014928 Serine_rich_dom
IPR038319 Serine_rich_sf
IPR036028 SH3-like_dom_sf
IPR001452 SH3_domain
PANTHERiPTHR10654 PTHR10654, 1 hit
PTHR10654:SF15 PTHR10654:SF15, 1 hit
PfamiView protein in Pfam
PF12026 DUF3513, 1 hit
PF08824 Serine_rich, 1 hit
PF00018 SH3_1, 1 hit
PRINTSiPR00452 SH3DOMAIN
SMARTiView protein in SMART
SM00326 SH3, 1 hit
SUPFAMiSSF50044 SSF50044, 1 hit
PROSITEiView protein in PROSITE
PS50002 SH3, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform Cas-B (identifier: Q61140-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MTVPNVLAKA LYDNVAESPD ELSFRKGDIM TVLERDTQGL DGWWLCSLHG 
        60         70         80         90        100
RQGIVPGNRL KILVGMYDKK PVGPGPGPPA TPPQPQPSLP QGVHAPVPPA 
       110        120        130        140        150
SQYSPMLPTA YQPQSDNVYL VPTPSKTQQG LYQAPGPNPQ FQSPPAKQTS 
       160        170        180        190        200
TFSKQTPHHS FPSPATDLYQ VPPGPGSPAQ DIYQVPPSAG IGHDIYQVPP 
       210        220        230        240        250
SLDTRGWEGT KPPAKVVVPT RVGQGYVYEA AQTEQDEYDT PRHLLAPGPQ 
       260        270        280        290        300
DIYDVPPVRG LLPNQYGQEV YDTPPMAVKG PNGRDPLLDV YDVPPSVEKG 
       310        320        330        340        350
LLSSSHHSVY DVPPSVSKDV PDGPLLREET YDVPPAFAKP KPFDPTRHPL 
       360        370        380        390        400
ILAAPPPDSP AAEDVYDVPP PAPDLYDVPP GLRRPGPGTL YDVPRERVLP 
       410        420        430        440        450
PEVADGSVVD DGVYAVPPPA EREAPTDGKR LSASSTGSTR SSQSASSLEV 
       460        470        480        490        500
VVPGREPLEL EVAVESLARL QQGVSTTVAH LLDLVGSASG PGGWRGTSEP 
       510        520        530        540        550
QEPPAQDLKA AVAAVHGAVH ELLEFARGAV SNATHTSDRT LHAKLSRQLQ 
       560        570        580        590        600
KMEDVYQTLV VHGQVLDSGR GSPGFTPEDL DRLVACSRAV PEDAKQLASF 
       610        620        630        640        650
LHGNASLLFR RTKAPGPGPE GSSSLHPNPT DKASSIQSRP LPSPPKFTSQ 
       660        670        680        690        700
DSPDGQYENS EGGWMEDYDY VHLQGKEEFE KTQKELLERG NIMRQGKGQL 
       710        720        730        740        750
ELQQLKQFER LEQEVSRPID HDLANWTPAQ PLVPGRTGGL GPSDRQLLLF 
       760        770        780        790        800
YLEQCEANLT TLTDAVDAFF TAVATNQPPK IFVAHSKFVI LSAHKLVFIG 
       810        820        830        840        850
DTLSRQAKAA DVRSQVTHYS NLLCDLLRGI VATTKAAALQ YPSPSAAQDM 
       860        870 
VDRVKELGHS TQQFRRVLGQ LAAA
Length:874
Mass (Da):94,285
Last modified:July 27, 2011 - v2
Checksum:i81E56BC7AD87B095
GO
Isoform Cas-A (identifier: Q61140-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-4: MTVP → MKYL

Show »
Length:874
Mass (Da):94,392
Checksum:i25B8F5D1D61CC8B1
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti72V → A in AAA93381 (PubMed:7479864).Curated1
Sequence conflicti72V → A in AAA93248 (PubMed:7479864).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0041341 – 4MTVP → MKYL in isoform Cas-A. 1 Publication4

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U48853 mRNA Translation: AAA93381.1
U28151 mRNA Translation: AAA93248.1
AK145863 mRNA Translation: BAE26705.1
BC057578 mRNA Translation: AAH57578.1
CCDSiCCDS52675.1 [Q61140-1]
CCDS85616.1 [Q61140-2]
RefSeqiNP_001185768.1, NM_001198839.1 [Q61140-2]
NP_034084.2, NM_009954.3 [Q61140-1]
UniGeneiMm.3758

Genome annotation databases

EnsembliENSMUST00000166232; ENSMUSP00000129584; ENSMUSG00000031955 [Q61140-1]
ENSMUST00000212349; ENSMUSP00000148364; ENSMUSG00000031955 [Q61140-2]
GeneIDi12927
KEGGimmu:12927
UCSCiuc009nmt.2 mouse [Q61140-2]
uc009nmu.2 mouse [Q61140-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiBCAR1_MOUSE
AccessioniPrimary (citable) accession number: Q61140
Secondary accession number(s): Q60869, Q6PFF9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: July 27, 2011
Last modified: June 20, 2018
This is version 171 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

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