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Q61139 (PCSK7_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 125. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Proprotein convertase subtilisin/kexin type 7

EC=3.4.21.-
Alternative name(s):
Prohormone convertase 7
Proprotein convertase 7
Short name=PC7
Subtilisin-like proprotein convertase 7
Short name=SPC7
Subtilisin/kexin-like protease PC7
Gene names
Name:Pcsk7
Synonyms:Pc7
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length770 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Likely to represent a ubiquitous endoprotease activity within constitutive secretory pathways and capable of cleavage at the RXXX[KR]R consensus motif.

Catalytic activity

Release of mature proteins from their proproteins by cleavage of Arg-Xaa-Xaa-Xaa-Yaa-Arg-|-Zaa bonds, where Xaa can be any amino acid and Yaa is Arg or Lys.

Cofactor

Calcium By similarity.

Enzyme regulation

Inhibited by zinc and copper By similarity.

Subcellular location

Golgi apparatustrans-Golgi network membrane; Single-pass type I membrane protein By similarity. Note: Seems to be localized intracellularly to the trans Golgi network By similarity.

Tissue specificity

Widely expressed. Expressed in brain, lung, muscle, heart, liver, kidney, spleen and thymus.

Developmental stage

Ubiquitously expressed at constitutive levels during embryogenesis. Ref.1

Sequence similarities

Belongs to the peptidase S8 family.

Contains 1 homo B/P domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 3636 By similarity
Propeptide37 – 140104 By similarity
PRO_0000027116
Chain141 – 770630Proprotein convertase subtilisin/kexin type 7
PRO_0000027117

Regions

Topological domain141 – 666526Extracellular Potential
Transmembrane667 – 68721Helical; Potential
Topological domain688 – 77083Cytoplasmic Potential
Domain480 – 616137Homo B/P
Region141 – 472332Catalytic

Sites

Active site1861Charge relay system By similarity
Active site2271Charge relay system By similarity
Active site4051Charge relay system By similarity
Site140 – 1412Cleavage; by autolysis By similarity

Amino acid modifications

Glycosylation1661N-linked (GlcNAc...) Potential
Glycosylation1741N-linked (GlcNAc...) Potential
Glycosylation2401N-linked (GlcNAc...) Potential
Glycosylation5101N-linked (GlcNAc...) Potential

Experimental info

Sequence conflict191 – 1933HTV → THR in AAB09725. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q61139 [UniParc].

Last modified June 28, 2011. Version 2.
Checksum: E612EB6B7A0E6BFA

FASTA77084,358
        10         20         30         40         50         60 
MPKGRQKVPH LDAHLGLPIC LWLELAIFFL VPQVMGLSEA GGLDILGTGG LSWAVHLDSL 

        70         80         90        100        110        120 
EGERKEESLT QQADAVAQAA GLVNAGRIGE LQGHYLFVQP TGHRQAMEVE AMRQQAEAVL 

       130        140        150        160        170        180 
ARHEAVRWHS EQTLLKRAKR SIHFNDPKYP QQWHLNNRRS PGRDINVTGV WERNVTGRGV 

       190        200        210        220        230        240 
TVVVVDDGVE HTVQDIAPNY SPEGSYDLNS NDPDPMPHPD EENGNHHGTR CAGEIAAVPN 

       250        260        270        280        290        300 
NSFCAVGVAY GSRIAGIRVL DGPLTDSMEA VAFNKHYQIN DIYSCSWGPD DDGKTVDGPH 

       310        320        330        340        350        360 
QLGKAALQHG VMAGRQGFGS IFVVASGNGG QHNDNCNYDG YANSIYTVTI GAVDEEGRMP 

       370        380        390        400        410        420 
FYAEECASML AVTFSGGDKM LRSIVTTDWD LQKGTGCTEG HTGTSAAAPL AAGMIALMLQ 

       430        440        450        460        470        480 
VRPCLTWRDV QHIIVFTAIQ YEDHHADWLT NEAGFSHSHQ HGFGLLNAWR LVNAAKIWTS 

       490        500        510        520        530        540 
VPYLASYVSP MLKENKAVPR SPHSLEVLWN VSRTDLEMSG LKTLEHVAVT VSITHPRRGS 

       550        560        570        580        590        600 
LELKLFCPSG MMSLIGAPRS MDSDPNGFNA WTFSTVRCWG ERARGVYRLV IRDVGDEPLQ 

       610        620        630        640        650        660 
MGILQQWQLT LYGSMWSPVD IKDRQSLLES AMSGKYLHDG FTLPCPPGLK IPEEDGYTIT 

       670        680        690        700        710        720 
PNTLKTLVLV GCFSVFWTIY YMLEVCLSQR NKASTHGCRK GCCPWAPRRQ NSKDAGTALE 

       730        740        750        760        770 
SMPLCSSKDL DGVDSEHGDC TTASSFLAPE LDCPPHQPPD LLQGKSGQIC 

« Hide

References

« Hide 'large scale' references
[1]"SPC4, SPC6, and the novel protease SPC7 are coexpressed with bone morphogenetic proteins at distinct sites during embryogenesis."
Constam D.B., Calfon M., Robertson E.J.
J. Cell Biol. 134:181-191(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], DEVELOPMENTAL STAGE.
Strain: C57BL/6.
Tissue: Embryo.
[2]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6J.
Tissue: Spleen.
[3]Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: FVB/N.
Tissue: Mammary tumor.
[5]Ebihara M., Meerabux J., Young B.D.
Submitted (OCT-1996) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-384.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U48830 mRNA. Translation: AAB09725.1.
AK148416 mRNA. Translation: BAE28541.1.
AK165389 mRNA. Translation: BAE38156.1.
CH466522 Genomic DNA. Translation: EDL25666.1.
BC006730 mRNA. Translation: AAH06730.1.
U75902 Genomic DNA. Translation: AAB51126.1.
RefSeqNP_001268863.1. NM_001281934.1.
NP_032820.2. NM_008794.2.
UniGeneMm.283283.
Mm.3255.

3D structure databases

ProteinModelPortalQ61139.
SMRQ61139. Positions 50-133, 146-614.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10090.ENSMUSP00000047508.

Protein family/group databases

MEROPSS08.077.

PTM databases

PhosphoSiteQ61139.

Proteomic databases

PRIDEQ61139.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000039059; ENSMUSP00000047508; ENSMUSG00000035382.
GeneID18554.
KEGGmmu:18554.
UCSCuc009pgp.1. mouse.

Organism-specific databases

CTD9159.
MGIMGI:107421. Pcsk7.

Phylogenomic databases

eggNOGCOG4935.
GeneTreeENSGT00750000117689.
HOGENOMHOG000192536.
HOVERGENHBG008705.
InParanoidQ922W4.
KOK08673.
OMASGPCHWP.
OrthoDBEOG73JKV3.
TreeFamTF314277.

Enzyme and pathway databases

ReactomeREACT_188257. Signal Transduction.

Gene expression databases

BgeeQ61139.
CleanExMM_PCSK7.
GenevestigatorQ61139.

Family and domain databases

Gene3D2.60.120.260. 1 hit.
3.40.50.200. 1 hit.
InterProIPR008979. Galactose-bd-like.
IPR000209. Peptidase_S8/S53_dom.
IPR022398. Peptidase_S8_His-AS.
IPR023828. Peptidase_S8_Ser-AS.
IPR015500. Peptidase_S8_subtilisin-rel.
IPR009020. Prot_inh_propept.
IPR002884. PrprotnconvertsP.
[Graphical view]
PANTHERPTHR10795. PTHR10795. 1 hit.
PfamPF01483. P_proprotein. 1 hit.
PF00082. Peptidase_S8. 1 hit.
[Graphical view]
PRINTSPR00723. SUBTILISIN.
SUPFAMSSF49785. SSF49785. 1 hit.
SSF52743. SSF52743. 1 hit.
SSF54897. SSF54897. 1 hit.
PROSITEPS00137. SUBTILASE_HIS. 1 hit.
PS00138. SUBTILASE_SER. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio294372.
PROQ61139.
SOURCESearch...

Entry information

Entry namePCSK7_MOUSE
AccessionPrimary (citable) accession number: Q61139
Secondary accession number(s): O08577, Q922W4
Entry history
Integrated into UniProtKB/Swiss-Prot: January 11, 2001
Last sequence update: June 28, 2011
Last modified: April 16, 2014
This is version 125 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot