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Protein

Proprotein convertase subtilisin/kexin type 7

Gene

Pcsk7

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Likely to represent a ubiquitous endoprotease activity within constitutive secretory pathways and capable of cleavage at the RXXX[KR]R consensus motif.

Catalytic activityi

Release of mature proteins from their proproteins by cleavage of Arg-Xaa-Xaa-Xaa-Yaa-Arg-|-Zaa bonds, where Xaa can be any amino acid and Yaa is Arg or Lys.

Cofactori

Ca2+By similarity

Enzyme regulationi

Inhibited by zinc and copper.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei186Charge relay systemBy similarity1
Active sitei227Charge relay systemBy similarity1
Active sitei405Charge relay systemBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Enzyme and pathway databases

ReactomeiR-MMU-167060. NGF processing.

Protein family/group databases

MEROPSiS08.077.

Names & Taxonomyi

Protein namesi
Recommended name:
Proprotein convertase subtilisin/kexin type 7 (EC:3.4.21.-)
Alternative name(s):
Prohormone convertase 7
Proprotein convertase 7
Short name:
PC7
Subtilisin-like proprotein convertase 7
Short name:
SPC7
Subtilisin/kexin-like protease PC7
Gene namesi
Name:Pcsk7
Synonyms:Pc7
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 9

Organism-specific databases

MGIiMGI:107421. Pcsk7.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini141 – 666ExtracellularSequence analysisAdd BLAST526
Transmembranei667 – 687HelicalSequence analysisAdd BLAST21
Topological domaini688 – 770CytoplasmicSequence analysisAdd BLAST83

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 36By similarityAdd BLAST36
PropeptideiPRO_000002711637 – 140By similarityAdd BLAST104
ChainiPRO_0000027117141 – 770Proprotein convertase subtilisin/kexin type 7Add BLAST630

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi166N-linked (GlcNAc...)Sequence analysis1
Glycosylationi174N-linked (GlcNAc...)Sequence analysis1
Glycosylationi240N-linked (GlcNAc...)Sequence analysis1
Glycosylationi510N-linked (GlcNAc...)Sequence analysis1

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei140 – 141Cleavage; by autolysisBy similarity2

Keywords - PTMi

Cleavage on pair of basic residues, Glycoprotein, Zymogen

Proteomic databases

PaxDbiQ61139.
PRIDEiQ61139.

PTM databases

iPTMnetiQ61139.
PhosphoSitePlusiQ61139.

Expressioni

Tissue specificityi

Widely expressed. Expressed in brain, lung, muscle, heart, liver, kidney, spleen and thymus.

Developmental stagei

Ubiquitously expressed at constitutive levels during embryogenesis.1 Publication

Gene expression databases

BgeeiENSMUSG00000035382.
CleanExiMM_PCSK7.
GenevisibleiQ61139. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000047508.

Structurei

3D structure databases

ProteinModelPortaliQ61139.
SMRiQ61139.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini181 – 473Peptidase S8Add BLAST293

Sequence similaritiesi

Belongs to the peptidase S8 family.Curated
Contains 1 peptidase S8 domain.Curated

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3525. Eukaryota.
COG1404. LUCA.
COG4935. LUCA.
GeneTreeiENSGT00750000117358.
HOGENOMiHOG000192536.
HOVERGENiHBG008705.
InParanoidiQ61139.
KOiK08673.
OMAiHEAVRWH.
OrthoDBiEOG091G01XU.
TreeFamiTF314277.

Family and domain databases

Gene3Di2.60.120.260. 1 hit.
3.30.70.850. 1 hit.
3.40.50.200. 1 hit.
InterProiIPR008979. Galactose-bd-like.
IPR000209. Peptidase_S8/S53_dom.
IPR022398. Peptidase_S8_His-AS.
IPR023828. Peptidase_S8_Ser-AS.
IPR015500. Peptidase_S8_subtilisin-rel.
IPR009020. Propept_inh.
IPR002884. PrprotnconvertsP.
IPR032815. S8_pro-domain.
[Graphical view]
PANTHERiPTHR10795. PTHR10795. 1 hit.
PfamiPF01483. P_proprotein. 1 hit.
PF00082. Peptidase_S8. 1 hit.
PF16470. S8_pro-domain. 1 hit.
[Graphical view]
PRINTSiPR00723. SUBTILISIN.
SUPFAMiSSF49785. SSF49785. 1 hit.
SSF52743. SSF52743. 1 hit.
SSF54897. SSF54897. 1 hit.
PROSITEiPS00137. SUBTILASE_HIS. 1 hit.
PS00138. SUBTILASE_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q61139-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPKGRQKVPH LDAHLGLPIC LWLELAIFFL VPQVMGLSEA GGLDILGTGG
60 70 80 90 100
LSWAVHLDSL EGERKEESLT QQADAVAQAA GLVNAGRIGE LQGHYLFVQP
110 120 130 140 150
TGHRQAMEVE AMRQQAEAVL ARHEAVRWHS EQTLLKRAKR SIHFNDPKYP
160 170 180 190 200
QQWHLNNRRS PGRDINVTGV WERNVTGRGV TVVVVDDGVE HTVQDIAPNY
210 220 230 240 250
SPEGSYDLNS NDPDPMPHPD EENGNHHGTR CAGEIAAVPN NSFCAVGVAY
260 270 280 290 300
GSRIAGIRVL DGPLTDSMEA VAFNKHYQIN DIYSCSWGPD DDGKTVDGPH
310 320 330 340 350
QLGKAALQHG VMAGRQGFGS IFVVASGNGG QHNDNCNYDG YANSIYTVTI
360 370 380 390 400
GAVDEEGRMP FYAEECASML AVTFSGGDKM LRSIVTTDWD LQKGTGCTEG
410 420 430 440 450
HTGTSAAAPL AAGMIALMLQ VRPCLTWRDV QHIIVFTAIQ YEDHHADWLT
460 470 480 490 500
NEAGFSHSHQ HGFGLLNAWR LVNAAKIWTS VPYLASYVSP MLKENKAVPR
510 520 530 540 550
SPHSLEVLWN VSRTDLEMSG LKTLEHVAVT VSITHPRRGS LELKLFCPSG
560 570 580 590 600
MMSLIGAPRS MDSDPNGFNA WTFSTVRCWG ERARGVYRLV IRDVGDEPLQ
610 620 630 640 650
MGILQQWQLT LYGSMWSPVD IKDRQSLLES AMSGKYLHDG FTLPCPPGLK
660 670 680 690 700
IPEEDGYTIT PNTLKTLVLV GCFSVFWTIY YMLEVCLSQR NKASTHGCRK
710 720 730 740 750
GCCPWAPRRQ NSKDAGTALE SMPLCSSKDL DGVDSEHGDC TTASSFLAPE
760 770
LDCPPHQPPD LLQGKSGQIC
Length:770
Mass (Da):84,358
Last modified:June 28, 2011 - v2
Checksum:iE612EB6B7A0E6BFA
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti191 – 193HTV → THR in AAB09725 (PubMed:8698813).Curated3

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U48830 mRNA. Translation: AAB09725.1.
AK148416 mRNA. Translation: BAE28541.1.
AK165389 mRNA. Translation: BAE38156.1.
CH466522 Genomic DNA. Translation: EDL25666.1.
BC006730 mRNA. Translation: AAH06730.1.
U75902 Genomic DNA. Translation: AAB51126.1.
CCDSiCCDS40609.1.
RefSeqiNP_001268863.1. NM_001281934.1.
NP_032820.2. NM_008794.2.
XP_017168699.1. XM_017313210.1.
UniGeneiMm.283283.
Mm.3255.

Genome annotation databases

EnsembliENSMUST00000039059; ENSMUSP00000047508; ENSMUSG00000035382.
GeneIDi18554.
KEGGimmu:18554.
UCSCiuc009pgp.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U48830 mRNA. Translation: AAB09725.1.
AK148416 mRNA. Translation: BAE28541.1.
AK165389 mRNA. Translation: BAE38156.1.
CH466522 Genomic DNA. Translation: EDL25666.1.
BC006730 mRNA. Translation: AAH06730.1.
U75902 Genomic DNA. Translation: AAB51126.1.
CCDSiCCDS40609.1.
RefSeqiNP_001268863.1. NM_001281934.1.
NP_032820.2. NM_008794.2.
XP_017168699.1. XM_017313210.1.
UniGeneiMm.283283.
Mm.3255.

3D structure databases

ProteinModelPortaliQ61139.
SMRiQ61139.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000047508.

Protein family/group databases

MEROPSiS08.077.

PTM databases

iPTMnetiQ61139.
PhosphoSitePlusiQ61139.

Proteomic databases

PaxDbiQ61139.
PRIDEiQ61139.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000039059; ENSMUSP00000047508; ENSMUSG00000035382.
GeneIDi18554.
KEGGimmu:18554.
UCSCiuc009pgp.1. mouse.

Organism-specific databases

CTDi9159.
MGIiMGI:107421. Pcsk7.

Phylogenomic databases

eggNOGiKOG3525. Eukaryota.
COG1404. LUCA.
COG4935. LUCA.
GeneTreeiENSGT00750000117358.
HOGENOMiHOG000192536.
HOVERGENiHBG008705.
InParanoidiQ61139.
KOiK08673.
OMAiHEAVRWH.
OrthoDBiEOG091G01XU.
TreeFamiTF314277.

Enzyme and pathway databases

ReactomeiR-MMU-167060. NGF processing.

Miscellaneous databases

PROiQ61139.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000035382.
CleanExiMM_PCSK7.
GenevisibleiQ61139. MM.

Family and domain databases

Gene3Di2.60.120.260. 1 hit.
3.30.70.850. 1 hit.
3.40.50.200. 1 hit.
InterProiIPR008979. Galactose-bd-like.
IPR000209. Peptidase_S8/S53_dom.
IPR022398. Peptidase_S8_His-AS.
IPR023828. Peptidase_S8_Ser-AS.
IPR015500. Peptidase_S8_subtilisin-rel.
IPR009020. Propept_inh.
IPR002884. PrprotnconvertsP.
IPR032815. S8_pro-domain.
[Graphical view]
PANTHERiPTHR10795. PTHR10795. 1 hit.
PfamiPF01483. P_proprotein. 1 hit.
PF00082. Peptidase_S8. 1 hit.
PF16470. S8_pro-domain. 1 hit.
[Graphical view]
PRINTSiPR00723. SUBTILISIN.
SUPFAMiSSF49785. SSF49785. 1 hit.
SSF52743. SSF52743. 1 hit.
SSF54897. SSF54897. 1 hit.
PROSITEiPS00137. SUBTILASE_HIS. 1 hit.
PS00138. SUBTILASE_SER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPCSK7_MOUSE
AccessioniPrimary (citable) accession number: Q61139
Secondary accession number(s): O08577, Q922W4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 11, 2001
Last sequence update: June 28, 2011
Last modified: November 2, 2016
This is version 149 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.