Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q61139

- PCSK7_MOUSE

UniProt

Q61139 - PCSK7_MOUSE

Protein

Proprotein convertase subtilisin/kexin type 7

Gene

Pcsk7

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 130 (01 Oct 2014)
      Sequence version 2 (28 Jun 2011)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Likely to represent a ubiquitous endoprotease activity within constitutive secretory pathways and capable of cleavage at the RXXX[KR]R consensus motif.

    Catalytic activityi

    Release of mature proteins from their proproteins by cleavage of Arg-Xaa-Xaa-Xaa-Yaa-Arg-|-Zaa bonds, where Xaa can be any amino acid and Yaa is Arg or Lys.

    Cofactori

    Calcium.By similarity

    Enzyme regulationi

    Inhibited by zinc and copper.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei140 – 1412Cleavage; by autolysisBy similarity
    Active sitei186 – 1861Charge relay systemBy similarity
    Active sitei227 – 2271Charge relay systemBy similarity
    Active sitei405 – 4051Charge relay systemBy similarity

    GO - Molecular functioni

    1. peptidase activity Source: MGI
    2. serine-type endopeptidase activity Source: RefGenome

    GO - Biological processi

    1. protein processing Source: MGI

    Keywords - Molecular functioni

    Hydrolase, Protease, Serine protease

    Enzyme and pathway databases

    ReactomeiREACT_206713. NGF processing.

    Protein family/group databases

    MEROPSiS08.077.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Proprotein convertase subtilisin/kexin type 7 (EC:3.4.21.-)
    Alternative name(s):
    Prohormone convertase 7
    Proprotein convertase 7
    Short name:
    PC7
    Subtilisin-like proprotein convertase 7
    Short name:
    SPC7
    Subtilisin/kexin-like protease PC7
    Gene namesi
    Name:Pcsk7
    Synonyms:Pc7
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 9

    Organism-specific databases

    MGIiMGI:107421. Pcsk7.

    Subcellular locationi

    Golgi apparatustrans-Golgi network membrane By similarity; Single-pass type I membrane protein By similarity
    Note: Seems to be localized intracellularly to the trans Golgi network.By similarity

    GO - Cellular componenti

    1. endoplasmic reticulum lumen Source: Reactome
    2. Golgi apparatus Source: RefGenome
    3. integral component of Golgi membrane Source: Ensembl

    Keywords - Cellular componenti

    Golgi apparatus, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 3636By similarityAdd
    BLAST
    Propeptidei37 – 140104By similarityPRO_0000027116Add
    BLAST
    Chaini141 – 770630Proprotein convertase subtilisin/kexin type 7PRO_0000027117Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi166 – 1661N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi174 – 1741N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi240 – 2401N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi510 – 5101N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Cleavage on pair of basic residues, Glycoprotein, Zymogen

    Proteomic databases

    PRIDEiQ61139.

    PTM databases

    PhosphoSiteiQ61139.

    Expressioni

    Tissue specificityi

    Widely expressed. Expressed in brain, lung, muscle, heart, liver, kidney, spleen and thymus.

    Developmental stagei

    Ubiquitously expressed at constitutive levels during embryogenesis.1 Publication

    Gene expression databases

    BgeeiQ61139.
    CleanExiMM_PCSK7.
    GenevestigatoriQ61139.

    Interactioni

    Protein-protein interaction databases

    STRINGi10090.ENSMUSP00000047508.

    Structurei

    3D structure databases

    ProteinModelPortaliQ61139.
    SMRiQ61139. Positions 146-614.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini141 – 666526ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini688 – 77083CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei667 – 68721HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini181 – 473293Peptidase S8Add
    BLAST

    Sequence similaritiesi

    Belongs to the peptidase S8 family.Curated
    Contains 1 peptidase S8 domain.Curated

    Keywords - Domaini

    Signal, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG4935.
    GeneTreeiENSGT00750000117689.
    HOGENOMiHOG000192536.
    HOVERGENiHBG008705.
    InParanoidiQ922W4.
    KOiK08673.
    OMAiSGPCHWP.
    OrthoDBiEOG73JKV3.
    TreeFamiTF314277.

    Family and domain databases

    Gene3Di2.60.120.260. 1 hit.
    3.40.50.200. 1 hit.
    InterProiIPR008979. Galactose-bd-like.
    IPR000209. Peptidase_S8/S53_dom.
    IPR022398. Peptidase_S8_His-AS.
    IPR023828. Peptidase_S8_Ser-AS.
    IPR015500. Peptidase_S8_subtilisin-rel.
    IPR009020. Prot_inh_propept.
    IPR002884. PrprotnconvertsP.
    [Graphical view]
    PANTHERiPTHR10795. PTHR10795. 1 hit.
    PfamiPF01483. P_proprotein. 1 hit.
    PF00082. Peptidase_S8. 1 hit.
    [Graphical view]
    PRINTSiPR00723. SUBTILISIN.
    SUPFAMiSSF49785. SSF49785. 1 hit.
    SSF52743. SSF52743. 1 hit.
    SSF54897. SSF54897. 1 hit.
    PROSITEiPS00137. SUBTILASE_HIS. 1 hit.
    PS00138. SUBTILASE_SER. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q61139-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPKGRQKVPH LDAHLGLPIC LWLELAIFFL VPQVMGLSEA GGLDILGTGG    50
    LSWAVHLDSL EGERKEESLT QQADAVAQAA GLVNAGRIGE LQGHYLFVQP 100
    TGHRQAMEVE AMRQQAEAVL ARHEAVRWHS EQTLLKRAKR SIHFNDPKYP 150
    QQWHLNNRRS PGRDINVTGV WERNVTGRGV TVVVVDDGVE HTVQDIAPNY 200
    SPEGSYDLNS NDPDPMPHPD EENGNHHGTR CAGEIAAVPN NSFCAVGVAY 250
    GSRIAGIRVL DGPLTDSMEA VAFNKHYQIN DIYSCSWGPD DDGKTVDGPH 300
    QLGKAALQHG VMAGRQGFGS IFVVASGNGG QHNDNCNYDG YANSIYTVTI 350
    GAVDEEGRMP FYAEECASML AVTFSGGDKM LRSIVTTDWD LQKGTGCTEG 400
    HTGTSAAAPL AAGMIALMLQ VRPCLTWRDV QHIIVFTAIQ YEDHHADWLT 450
    NEAGFSHSHQ HGFGLLNAWR LVNAAKIWTS VPYLASYVSP MLKENKAVPR 500
    SPHSLEVLWN VSRTDLEMSG LKTLEHVAVT VSITHPRRGS LELKLFCPSG 550
    MMSLIGAPRS MDSDPNGFNA WTFSTVRCWG ERARGVYRLV IRDVGDEPLQ 600
    MGILQQWQLT LYGSMWSPVD IKDRQSLLES AMSGKYLHDG FTLPCPPGLK 650
    IPEEDGYTIT PNTLKTLVLV GCFSVFWTIY YMLEVCLSQR NKASTHGCRK 700
    GCCPWAPRRQ NSKDAGTALE SMPLCSSKDL DGVDSEHGDC TTASSFLAPE 750
    LDCPPHQPPD LLQGKSGQIC 770
    Length:770
    Mass (Da):84,358
    Last modified:June 28, 2011 - v2
    Checksum:iE612EB6B7A0E6BFA
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti191 – 1933HTV → THR in AAB09725. (PubMed:8698813)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U48830 mRNA. Translation: AAB09725.1.
    AK148416 mRNA. Translation: BAE28541.1.
    AK165389 mRNA. Translation: BAE38156.1.
    CH466522 Genomic DNA. Translation: EDL25666.1.
    BC006730 mRNA. Translation: AAH06730.1.
    U75902 Genomic DNA. Translation: AAB51126.1.
    CCDSiCCDS40609.1.
    RefSeqiNP_001268863.1. NM_001281934.1.
    NP_032820.2. NM_008794.2.
    UniGeneiMm.283283.
    Mm.3255.

    Genome annotation databases

    EnsembliENSMUST00000039059; ENSMUSP00000047508; ENSMUSG00000035382.
    GeneIDi18554.
    KEGGimmu:18554.
    UCSCiuc009pgp.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U48830 mRNA. Translation: AAB09725.1 .
    AK148416 mRNA. Translation: BAE28541.1 .
    AK165389 mRNA. Translation: BAE38156.1 .
    CH466522 Genomic DNA. Translation: EDL25666.1 .
    BC006730 mRNA. Translation: AAH06730.1 .
    U75902 Genomic DNA. Translation: AAB51126.1 .
    CCDSi CCDS40609.1.
    RefSeqi NP_001268863.1. NM_001281934.1.
    NP_032820.2. NM_008794.2.
    UniGenei Mm.283283.
    Mm.3255.

    3D structure databases

    ProteinModelPortali Q61139.
    SMRi Q61139. Positions 146-614.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10090.ENSMUSP00000047508.

    Protein family/group databases

    MEROPSi S08.077.

    PTM databases

    PhosphoSitei Q61139.

    Proteomic databases

    PRIDEi Q61139.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000039059 ; ENSMUSP00000047508 ; ENSMUSG00000035382 .
    GeneIDi 18554.
    KEGGi mmu:18554.
    UCSCi uc009pgp.1. mouse.

    Organism-specific databases

    CTDi 9159.
    MGIi MGI:107421. Pcsk7.

    Phylogenomic databases

    eggNOGi COG4935.
    GeneTreei ENSGT00750000117689.
    HOGENOMi HOG000192536.
    HOVERGENi HBG008705.
    InParanoidi Q922W4.
    KOi K08673.
    OMAi SGPCHWP.
    OrthoDBi EOG73JKV3.
    TreeFami TF314277.

    Enzyme and pathway databases

    Reactomei REACT_206713. NGF processing.

    Miscellaneous databases

    NextBioi 294372.
    PROi Q61139.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q61139.
    CleanExi MM_PCSK7.
    Genevestigatori Q61139.

    Family and domain databases

    Gene3Di 2.60.120.260. 1 hit.
    3.40.50.200. 1 hit.
    InterProi IPR008979. Galactose-bd-like.
    IPR000209. Peptidase_S8/S53_dom.
    IPR022398. Peptidase_S8_His-AS.
    IPR023828. Peptidase_S8_Ser-AS.
    IPR015500. Peptidase_S8_subtilisin-rel.
    IPR009020. Prot_inh_propept.
    IPR002884. PrprotnconvertsP.
    [Graphical view ]
    PANTHERi PTHR10795. PTHR10795. 1 hit.
    Pfami PF01483. P_proprotein. 1 hit.
    PF00082. Peptidase_S8. 1 hit.
    [Graphical view ]
    PRINTSi PR00723. SUBTILISIN.
    SUPFAMi SSF49785. SSF49785. 1 hit.
    SSF52743. SSF52743. 1 hit.
    SSF54897. SSF54897. 1 hit.
    PROSITEi PS00137. SUBTILASE_HIS. 1 hit.
    PS00138. SUBTILASE_SER. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "SPC4, SPC6, and the novel protease SPC7 are coexpressed with bone morphogenetic proteins at distinct sites during embryogenesis."
      Constam D.B., Calfon M., Robertson E.J.
      J. Cell Biol. 134:181-191(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], DEVELOPMENTAL STAGE.
      Strain: C57BL/6.
      Tissue: Embryo.
    2. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Spleen.
    3. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
      Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: FVB/N.
      Tissue: Mammary tumor.
    5. Ebihara M., Meerabux J., Young B.D.
      Submitted (OCT-1996) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-384.

    Entry informationi

    Entry nameiPCSK7_MOUSE
    AccessioniPrimary (citable) accession number: Q61139
    Secondary accession number(s): O08577, Q922W4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 11, 2001
    Last sequence update: June 28, 2011
    Last modified: October 1, 2014
    This is version 130 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. Peptidase families
      Classification of peptidase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3