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Protein

Astrotactin-1

Gene

Astn1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Neuronal adhesion molecule that is required for normal migration of young postmitotic neuroblasts along glial fibers, especially in the cerebellum. Required for normal rate of migration of granule cells during brain development and for normal cerebellum development.2 Publications

GO - Biological processi

  • cell-cell adhesion Source: MGI
  • cell migration Source: MGI
  • locomotory behavior Source: MGI
  • neuron cell-cell adhesion Source: MGI
  • neuron migration Source: MGI

Keywordsi

Biological processCell adhesion

Names & Taxonomyi

Protein namesi
Recommended name:
Astrotactin-1
Alternative name(s):
Neuronal migration protein GC14
Gene namesi
Name:Astn1
Synonyms:Astn, Kiaa0289
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:1098567 Astn1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini22 – 153ExtracellularCuratedAdd BLAST132
Transmembranei154 – 174HelicalSequence analysisAdd BLAST21
Topological domaini175 – 388CytoplasmicCuratedAdd BLAST214
Transmembranei389 – 409HelicalSequence analysisAdd BLAST21
Topological domaini410 – 1302ExtracellularCurated1 PublicationAdd BLAST893

Keywords - Cellular componenti

Cell membrane, Cytoplasmic vesicle, Endosome, Membrane

Pathology & Biotechi

Disruption phenotypei

Slower than normal migration of granule cells in the developing cerebellum, leading to a decreased cerebellum size in adult mice and impaired skills in tasks that require coordinated movement and balance. Granule cells from mutant mice have a rounded shape and lack the elongated shape seen in wild-type. At 6 days after birth, increased granule cell apoptosis is observed, contrary to the situation in wild-type.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 21Sequence analysisAdd BLAST21
ChainiPRO_000000748222 – 1302Astrotactin-1Add BLAST1281

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi115N-linked (GlcNAc...) asparagineSequence analysis1
Modified residuei227PhosphoserineCombined sources1
Modified residuei337Omega-N-methylarginineCombined sources1
Glycosylationi453N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi463 ↔ 475By similarity
Disulfide bondi471 ↔ 490By similarity
Disulfide bondi492 ↔ 506By similarity
Disulfide bondi612 ↔ 625By similarity
Disulfide bondi619 ↔ 636By similarity
Disulfide bondi638 ↔ 651By similarity
Disulfide bondi660 ↔ 672By similarity
Disulfide bondi668 ↔ 692By similarity
Disulfide bondi694 ↔ 707By similarity
Glycosylationi729N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi742N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi785 ↔ 951By similarity
Glycosylationi804N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi876 ↔ 941By similarity
Disulfide bondi947 ↔ 954By similarity
Glycosylationi984N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi1000 ↔ 1011By similarity
Disulfide bondi1013 ↔ 1026By similarity
Disulfide bondi1101 ↔ 1121By similarity
Disulfide bondi1153 ↔ 1240By similarity
Disulfide bondi1261 ↔ 1284By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Methylation, Phosphoprotein

Proteomic databases

PaxDbiQ61137
PRIDEiQ61137

PTM databases

iPTMnetiQ61137
PhosphoSitePlusiQ61137

Expressioni

Tissue specificityi

Detected in brain (at protein level) (PubMed:11861479). Expressed specifically in the brain. Expressed in the cerebellum, hippocampus, cerebrum and olfactory bulb (PubMed:8602532).2 Publications

Developmental stagei

First detected in embryonic brain at 12 dpc. Expression increases thereafter, is highest in brain from pups 4 days after birth, and then decreases again. Expression is decreased 14 days after birth, and not detectable in adult brain.1 Publication

Gene expression databases

BgeeiENSMUSG00000026587
CleanExiMM_ASTN1
ExpressionAtlasiQ61137 baseline and differential
GenevisibleiQ61137 MM

Interactioni

Subunit structurei

Interacts with ASTN2; the interaction is not calcium-dependent.1 Publication

Protein-protein interaction databases

IntActiQ61137, 2 interactors
MINTiQ61137

Structurei

3D structure databases

ProteinModelPortaliQ61137
SMRiQ61137
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini459 – 507EGF-like 1Add BLAST49
Domaini608 – 652EGF-like 2Add BLAST45
Domaini656 – 708EGF-like 3Add BLAST53
Domaini1030 – 1145Fibronectin type-IIIAdd BLAST116

Sequence similaritiesi

Belongs to the astrotactin family.Curated

Keywords - Domaini

EGF-like domain, Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IHIU Eukaryota
ENOG4110VB2 LUCA
GeneTreeiENSGT00390000003140
HOGENOMiHOG000034112
HOVERGENiHBG050597
InParanoidiQ61137
OMAiEESCSIW
OrthoDBiEOG091G00IB
TreeFamiTF332034

Family and domain databases

CDDicd00063 FN3, 1 hit
InterProiView protein in InterPro
IPR026995 Astrotactin
IPR000742 EGF-like_dom
IPR003961 FN3_dom
IPR036116 FN3_sf
IPR020864 MACPF
PANTHERiPTHR16592 PTHR16592, 1 hit
SMARTiView protein in SMART
SM00181 EGF, 3 hits
SM00457 MACPF, 1 hit
SUPFAMiSSF49265 SSF49265, 2 hits

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q61137-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MALAGLCALF ACCWGPAAVL ATAAGDVDPS KELECKLKSI TVSALPFLRE
60 70 80 90 100
NDLSIMHSPS ASEPKLLFSV RNDFPGEMVV VDDLENTELP YFVLEISGNT
110 120 130 140 150
EDIPLVRWRQ QWLENGTLLF HIHHQDGAPS LPGQDPTEEP QHESAEEELR
160 170 180 190 200
ILHISVMGGM IALLLSILCL VMILYTRRRW CKRRRVPQPQ KSASAEAANE
210 220 230 240 250
IHYIPSVLIG GHGRESLRNA RVQGHNSSGT LSIRETPILD GYEYDITDLR
260 270 280 290 300
HHLQRECMNG GEDFASQVTR TLDSLQGCNE KSGMDLTPGS DNAKLSLMNK
310 320 330 340 350
YKDNIIATSP VDSNHQQATL LSHTSSSQRK RINNKARAGS AFLNPEGDSS
360 370 380 390 400
TEAENDPQLT FYTDPSRSRR RSRVGSPRSP VNKTTLTLIS VTSCVIGLVC
410 420 430 440 450
SSHVSCPLVV KITLHVPEHL IADGSRFILL EGSQLDASDW LNPAQVVLFS
460 470 480 490 500
QQNSSGPWAM DLCARRLLDP CEHQCDPETG RREHRAAGEC LCYEGYMKDP
510 520 530 540 550
VHKHLCIRNE WGTNQGPWPY TIFQRGFDLV LGEQPSDKIF RFTYTLGEGM
560 570 580 590 600
WLPLSKSFVI PPAELAINPS AKCKTDMTVM EDAVEVREEL MTSSSFDSLE
610 620 630 640 650
VLLDSFGPVR DCSKDNGGCS KNFRCISDRK LDSTGCVCPS GLSPMKDSSG
660 670 680 690 700
CYDRHIGVDC SDGFNGGCEQ LCLQQMAPFP EDPTLYNILM FCGCIEDYKL
710 720 730 740 750
GVDGRSCQLV TETCPEGGDC GESREVPMNQ TLFGEMFFGY NNQSKEVATG
760 770 780 790 800
QVLKGTFRQN NFARGLDQQL PDGLVVASVP LENQCLEEIS EPTPDPDFLT
810 820 830 840 850
GMVNFSEVSG YPVLQHWKVR SVMYHIKLNQ AAISQAFSNA LHSLDGATSR
860 870 880 890 900
ADFVALLDQF GNHYIQEAVY GFEESCSIWY PNKQVQRRLW LEYEDISKGN
910 920 930 940 950
SPSDESEERE RDPKVLTFPE YIASLSDSGT KRMAAGVRME CQSKGRCPSS
960 970 980 990 1000
CPLCHVTSSP ETPAEPVLLE VTRASPIYEL VTNNQTQRLL QEATMSSLWC
1010 1020 1030 1040 1050
SGTGDVIEDW CRCDSTAFGA DGLPTCAPLP QPVLRLSTVH EPSSNLVVLE
1060 1070 1080 1090 1100
WEHSEPPIGV QIVDYLIRQE KVTDRMDHSK VETETVLSFV DDIISGAKAP
1110 1120 1130 1140 1150
CAMPSQVPDK QLTTISLIIR CLEPDTIYMF TLWGVDNTGR RSRPSDVIVK
1160 1170 1180 1190 1200
TPCPVVDDVK AQEIADKIYN LFNGYTSGKE QQTAYNTLLD LGSPTLHRVL
1210 1220 1230 1240 1250
YHYNQHYESF GEFTWRCEDE LGPRKAGLIL SQLGDLSSWC NGLLQEPKIS
1260 1270 1280 1290 1300
LRRGSLKYLG CRYSEIKPYG LDWSELSRDL RKTCEEQTLS VPYNDYGDSK

DI
Length:1,302
Mass (Da):144,884
Last modified:October 3, 2012 - v4
Checksum:iAC917C4281401C97
GO
Isoform 2 (identifier: Q61137-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     480-487: Missing.

Note: No experimental confirmation available.
Show »
Length:1,294
Mass (Da):143,950
Checksum:i35EA0CADED41FFB1
GO

Sequence cautioni

The sequence AAC52516 differs from that shown. Reason: Frameshift at positions 84, 92, 185, 274, 370, 439, 469, 488, 592 and 595.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti216S → T in AAC52516 (PubMed:8602532).Curated1
Sequence conflicti369R → L in AAC52516 (PubMed:8602532).Curated1
Sequence conflicti396I → L in BAC41406 (PubMed:12465718).Curated1
Sequence conflicti421I → M in AAH94666 (PubMed:15489334).Curated1
Sequence conflicti437A → R in AAC52516 (PubMed:8602532).Curated1
Sequence conflicti681E → D in BAC41406 (PubMed:12465718).Curated1
Sequence conflicti1045N → T in BAC41406 (PubMed:12465718).Curated1
Sequence conflicti1067I → Y in AAC52516 (PubMed:8602532).Curated1
Sequence conflicti1214Missing in AAC52516 (PubMed:8602532).Curated1
Sequence conflicti1260 – 1261GC → AG in AAC52516 (PubMed:8602532).Curated2

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_014917480 – 487Missing in isoform 2. 2 Publications8

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC091266 Genomic DNA No translation available.
AC118728 Genomic DNA No translation available.
AC119867 Genomic DNA No translation available.
AC158910 Genomic DNA No translation available.
CH466520 Genomic DNA Translation: EDL39354.1
BC054545 mRNA Translation: AAH54545.1
BC094666 mRNA Translation: AAH94666.1
U48797 mRNA Translation: AAC52516.1 Frameshift.
AB093222 mRNA Translation: BAC41406.2
CCDSiCCDS56648.1 [Q61137-1]
CCDS78721.1 [Q61137-2]
RefSeqiNP_001192133.1, NM_001205204.1 [Q61137-1]
NP_031521.2, NM_007495.4 [Q61137-2]
UniGeneiMm.329586

Genome annotation databases

EnsembliENSMUST00000046110; ENSMUSP00000039711; ENSMUSG00000026587 [Q61137-2]
ENSMUST00000193042; ENSMUSP00000142322; ENSMUSG00000026587 [Q61137-1]
GeneIDi11899
KEGGimmu:11899
UCSCiuc007ddu.3 mouse [Q61137-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiASTN1_MOUSE
AccessioniPrimary (citable) accession number: Q61137
Secondary accession number(s): G5E8A1
, Q505A0, Q7TQG3, Q8CHH2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: October 3, 2012
Last modified: March 28, 2018
This is version 151 of the entry and version 4 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health