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Protein

Serine/threonine-protein kinase PRP4 homolog

Gene

Prpf4b

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Has a role in pre-mRNA splicing. Phosphorylates SF2/ASF.

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei717ATPPROSITE-ProRule annotation1
Active sitei815Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi693 – 701ATPPROSITE-ProRule annotation9

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • poly(A) RNA binding Source: MGI
  • protein kinase activity Source: MGI
  • protein serine/threonine kinase activity Source: UniProtKB-KW

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

mRNA processing, mRNA splicing

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.11.1. 3474.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase PRP4 homolog (EC:2.7.11.1)
Alternative name(s):
PRP4 pre-mRNA-processing factor 4 homolog
Pre-mRNA protein kinase
Gene namesi
Name:Prpf4b
Synonyms:Cbp143, Prp4h, Prp4k, Prp4m, Prpk
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 13

Organism-specific databases

MGIiMGI:109584. Prpf4b.

Subcellular locationi

GO - Cellular componenti

  • catalytic step 2 spliceosome Source: MGI
  • chromosome Source: MGI
  • nucleoplasm Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Nucleus, Spliceosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00000865872 – 1007Serine/threonine-protein kinase PRP4 homologAdd BLAST1006

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Modified residuei8PhosphoserineBy similarity1
Modified residuei21PhosphoserineCombined sources1
Modified residuei24PhosphoserineCombined sources1
Modified residuei33PhosphoserineCombined sources1
Modified residuei88PhosphoserineCombined sources1
Modified residuei94PhosphoserineCombined sources1
Modified residuei100N6-acetyllysineCombined sources1
Cross-linki118Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1)By similarity
Cross-linki118Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei132PhosphoserineBy similarity1
Modified residuei141PhosphotyrosineCombined sources1
Modified residuei143PhosphoserineCombined sources1
Modified residuei145PhosphoserineCombined sources1
Modified residuei167PhosphoserineBy similarity1
Cross-linki171Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei240PhosphoserineBy similarity1
Modified residuei242PhosphoserineBy similarity1
Modified residuei258PhosphoserineBy similarity1
Modified residuei278PhosphoserineBy similarity1
Modified residuei292PhosphoserineBy similarity1
Modified residuei294PhosphoserineBy similarity1
Modified residuei328PhosphoserineBy similarity1
Modified residuei354PhosphoserineBy similarity1
Modified residuei356PhosphoserineBy similarity1
Modified residuei366PhosphoserineBy similarity1
Modified residuei368PhosphoserineBy similarity1
Modified residuei385PhosphothreonineBy similarity1
Modified residuei387PhosphoserineBy similarity1
Modified residuei427PhosphoserineBy similarity1
Modified residuei431PhosphoserineCombined sources1
Modified residuei437PhosphoserineCombined sources1
Modified residuei518PhosphoserineBy similarity1
Modified residuei519PhosphoserineBy similarity1
Modified residuei520PhosphoserineBy similarity1
Modified residuei565PhosphoserineBy similarity1
Modified residuei569PhosphoserineBy similarity1
Modified residuei576PhosphoserineCombined sources1
Modified residuei578PhosphoserineCombined sources1
Modified residuei580PhosphoserineCombined sources1
Modified residuei717N6-acetyllysineBy similarity1
Modified residuei849PhosphotyrosineCombined sources1
Modified residuei852PhosphoserineBy similarity1

Post-translational modificationi

Phosphorylated by Clk1.

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ61136.
MaxQBiQ61136.
PaxDbiQ61136.
PRIDEiQ61136.

PTM databases

iPTMnetiQ61136.
PhosphoSitePlusiQ61136.

Expressioni

Gene expression databases

BgeeiENSMUSG00000021413.
GenevisibleiQ61136. MM.

Interactioni

Subunit structurei

Identified in the spliceosome C complex (By similarity). Interacts with Clk1 C-terminus.By similarity

Protein-protein interaction databases

BioGridi202400. 2 interactors.
IntActiQ61136. 3 interactors.
MINTiMINT-1751441.
STRINGi10090.ENSMUSP00000077019.

Structurei

3D structure databases

ProteinModelPortaliQ61136.
SMRiQ61136.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini687 – 1006Protein kinasePROSITE-ProRule annotationAdd BLAST320

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi40 – 497Arg/Lys-rich (basic)Add BLAST458
Compositional biasi41 – 79His-richAdd BLAST39

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0670. Eukaryota.
ENOG410XPAX. LUCA.
GeneTreeiENSGT00760000119032.
HOVERGENiHBG055182.
InParanoidiQ61136.
KOiK08827.
OMAiSGERDRY.
OrthoDBiEOG091G095F.
TreeFamiTF315246.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q61136-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAATEPPSLR EQAEMDDADN SEKSVNEENG EVSEDQSQNK HSRHKKKKHK
60 70 80 90 100
HRSKHKKHKH SSEEDRDKKH KHKHKHKKHK RKEVIEASDK EGLSPAKRTK
110 120 130 140 150
LDDLALLEDL EKQRALIKAE LDNELMEGKV QSGMGLILQG YESGSEEEGE
160 170 180 190 200
IHEKARNGNR SSTRSSSTRG KLEITDNKNS AKKRSKSRSK ERTRHRSDKR
210 220 230 240 250
KSKGAGEMLR EKANRSKSKE RRKSKSPSKR SKSQDQARKS KSPPLRRRSQ
260 270 280 290 300
EKVGKARSPA EEKMKSEEKG KIKDRKKSPI VNERSRDRSK KSKSPVDLRD
310 320 330 340 350
KSKDRRSRSK ERKSKRSEID KEKKPIKSPS KDASSGKENR SPSRRPGRSP
360 370 380 390 400
KRRSLSPKLR DKSRRSRSPL LNDRRSKQSK SPSRTLSPGR RAKSRSLERK
410 420 430 440 450
RREPERRRLS SPRTRPRDDI LGRCERSKDA SPINRWSPTR RRSRSPIRRR
460 470 480 490 500
SRSPLRRSRS PRRRSRSPRR RDRSRRSRSR LRRRSRSRGG HRRRSRSKVK
510 520 530 540 550
EDKFKGSLSE GMKVEQESSS DDNLEDFDVE EEDEEALIEQ RRIQRQAIVQ
560 570 580 590 600
KYKYLAEDSN ISVPSEPSSP QSSTRSRSPS PDDILERVAA DVKEYERENV
610 620 630 640 650
DTFEASVKAK HNLMTVEQNN GSSQKKILAP DMFTESDDMF AAYFDSARLR
660 670 680 690 700
AAGIGKDFKE NPNLRDNWTD AEGYYRVNIG EVLDKRYNVY GYTGQGVFSN
710 720 730 740 750
VVRARDNARA NQEVAVKIIR NNELMQKTGL KELEFLKKLN DADPDDKFHC
760 770 780 790 800
LRLFRHFYHK QHLCLVFEPL SMNLREVLKK YGKDVGLHIK AVRSYSQQLF
810 820 830 840 850
LALKLLKRCN ILHADIKPDN ILVNESKTIL KLCDFGSASH VADNDITPYL
860 870 880 890 900
VSRFYRAPEI IIGKSYDYGI DMWSVGCTLY ELYTGKILFP GKTNNHMLKL
910 920 930 940 950
AMDLKGKMPN KMIRKGVFKD QHFDQNLNFM YIEVDKVTER EKVTVMSTIN
960 970 980 990 1000
PTKDLLADLI GCQRLPEDQR KKVHQLKDLL DQILMLDPAK RISINQALQH

AFIQEKI
Length:1,007
Mass (Da):116,976
Last modified:July 27, 2011 - v3
Checksum:i18E1B7371E17AB4C
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti185 – 187SKS → IPG in AAC32042 (PubMed:9701556).Curated3
Sequence conflicti223K → I in AAC32042 (PubMed:9701556).Curated1
Sequence conflicti478R → K in AAM19102 (PubMed:12077342).Curated1
Sequence conflicti478R → K in AAC32042 (PubMed:9701556).Curated1
Sequence conflicti633F → L in AAB03269 (PubMed:9102632).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF283466 mRNA. Translation: AAM19102.1.
AK020579 mRNA. Translation: BAB32137.3.
AK021274 mRNA. Translation: BAB32358.3.
AK083926 mRNA. Translation: BAC39069.1.
CH466546 Genomic DNA. Translation: EDL40895.1.
BC141272 mRNA. Translation: AAI41273.1.
BC141273 mRNA. Translation: AAI41274.1.
AF033663 mRNA. Translation: AAC32042.1.
U48737 mRNA. Translation: AAB03269.1.
CCDSiCCDS26448.1.
RefSeqiNP_038858.2. NM_013830.2.
XP_017170914.1. XM_017315425.1.
XP_017170915.1. XM_017315426.1.
XP_017170916.1. XM_017315427.1.
XP_017170917.1. XM_017315428.1.
XP_017170918.1. XM_017315429.1.
XP_017170919.1. XM_017315430.1.
XP_017170920.1. XM_017315431.1.
XP_017170921.1. XM_017315432.1.
XP_017170922.1. XM_017315433.1.
XP_017170923.1. XM_017315434.1.
XP_017170924.1. XM_017315435.1.
XP_017170925.1. XM_017315436.1.
XP_017170926.1. XM_017315437.1.
UniGeneiMm.10027.
Mm.482253.

Genome annotation databases

EnsembliENSMUST00000077853; ENSMUSP00000077019; ENSMUSG00000021413.
GeneIDi19134.
KEGGimmu:19134.
UCSCiuc007qbp.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF283466 mRNA. Translation: AAM19102.1.
AK020579 mRNA. Translation: BAB32137.3.
AK021274 mRNA. Translation: BAB32358.3.
AK083926 mRNA. Translation: BAC39069.1.
CH466546 Genomic DNA. Translation: EDL40895.1.
BC141272 mRNA. Translation: AAI41273.1.
BC141273 mRNA. Translation: AAI41274.1.
AF033663 mRNA. Translation: AAC32042.1.
U48737 mRNA. Translation: AAB03269.1.
CCDSiCCDS26448.1.
RefSeqiNP_038858.2. NM_013830.2.
XP_017170914.1. XM_017315425.1.
XP_017170915.1. XM_017315426.1.
XP_017170916.1. XM_017315427.1.
XP_017170917.1. XM_017315428.1.
XP_017170918.1. XM_017315429.1.
XP_017170919.1. XM_017315430.1.
XP_017170920.1. XM_017315431.1.
XP_017170921.1. XM_017315432.1.
XP_017170922.1. XM_017315433.1.
XP_017170923.1. XM_017315434.1.
XP_017170924.1. XM_017315435.1.
XP_017170925.1. XM_017315436.1.
XP_017170926.1. XM_017315437.1.
UniGeneiMm.10027.
Mm.482253.

3D structure databases

ProteinModelPortaliQ61136.
SMRiQ61136.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi202400. 2 interactors.
IntActiQ61136. 3 interactors.
MINTiMINT-1751441.
STRINGi10090.ENSMUSP00000077019.

PTM databases

iPTMnetiQ61136.
PhosphoSitePlusiQ61136.

Proteomic databases

EPDiQ61136.
MaxQBiQ61136.
PaxDbiQ61136.
PRIDEiQ61136.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000077853; ENSMUSP00000077019; ENSMUSG00000021413.
GeneIDi19134.
KEGGimmu:19134.
UCSCiuc007qbp.2. mouse.

Organism-specific databases

CTDi8899.
MGIiMGI:109584. Prpf4b.

Phylogenomic databases

eggNOGiKOG0670. Eukaryota.
ENOG410XPAX. LUCA.
GeneTreeiENSGT00760000119032.
HOVERGENiHBG055182.
InParanoidiQ61136.
KOiK08827.
OMAiSGERDRY.
OrthoDBiEOG091G095F.
TreeFamiTF315246.

Enzyme and pathway databases

BRENDAi2.7.11.1. 3474.

Miscellaneous databases

ChiTaRSiPrpf4b. mouse.
PROiQ61136.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000021413.
GenevisibleiQ61136. MM.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPRP4B_MOUSE
AccessioniPrimary (citable) accession number: Q61136
Secondary accession number(s): B2RUN6
, O88378, Q8BND8, Q8R4Y5, Q9CTL9, Q9CTT0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: July 27, 2011
Last modified: November 2, 2016
This is version 153 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.