Reviewed, UniProtKB/Swiss-Prot Q61129 (CFAI_MOUSE)

Names and origin

Protein names

Recommended name:
    Complement factor I
    EC=3.4.21.45
Alternative name(s):
    C3B/C4B inactivator
Cleaved into the following 2 chains:
    1- Recommended name:
            Complement factor I heavy chain
    2- Recommended name:
            Complement factor I light chain

Gene names

Name:	Cfi
Synonyms:	If

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	603 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Responsible for cleaving the alpha-chains of C4b and C3b in the presence of the cofactors C4-binding protein and factor H respectively.
Catalytic activity	Inactivates complement subcomponents C3b, iC3b and C4b by proteolytic cleavage.
Subunit structure	Heterodimer of a light and heavy chains linked by disulfide bonds.
Subcellular location	Secreted › extracellular space.
Tissue specificity	Plasma.
Sequence similarities	Belongs to the peptidase S1 family. Contains 1 Kazal-like domain. Contains 2 LDL-receptor class A domains. Contains 1 peptidase S1 domain. Contains 1 SRCR domain.

Ontologies

Keywords
Biological process	Complement pathway Immune response Innate immunity
Cellular component	Secreted
Domain	Repeat Signal
Molecular function	Hydrolase Protease Serine protease
PTM	Cleavage on pair of basic residues Disulfide bond Glycoprotein
Gene Ontology (GO)
Biological process	complement activation, classical pathway Inferred from electronic annotation. Source: UniProtKB-KW innate immune response Inferred from electronic annotation. Source: UniProtKB-KW proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	extracellular space Inferred from electronic annotation. Source: UniProtKB-SubCell membrane Inferred from electronic annotation. Source: InterPro
Molecular function	scavenger receptor activity Inferred from electronic annotation. Source: InterPro serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 18	18	Potential
	Chain	19 – 603	585	Complement factor I		PRO_0000027571
	Chain	19 – 356	338	Complement factor I heavy chain		PRO_0000027572
	Chain	361 – 603	243	Complement factor I light chain		PRO_0000027573
Regions
	Domain	63 – 109	47	Kazal-like
	Domain	117 – 217	101	SRCR
	Domain	218 – 262	45	LDL-receptor class A 1
	Domain	263 – 299	37	LDL-receptor class A 2
	Domain	361 – 594	234	Peptidase S1
Sites
	Active site	401	1	Charge relay system By similarity
	Active site	449	1	Charge relay system By similarity
	Active site	545	1	Charge relay system By similarity
Amino acid modifications
	Glycosylation	106	1	N-linked (GlcNAc...) Potential
	Glycosylation	116	1	N-linked (GlcNAc...) Ref.3
	Glycosylation	174	1	N-linked (GlcNAc...) Potential
	Glycosylation	182	1	N-linked (GlcNAc...) Potential
	Glycosylation	267	1	N-linked (GlcNAc...) Potential
	Glycosylation	514	1	N-linked (GlcNAc...) Ref.4
	Glycosylation	556	1	N-linked (GlcNAc...) Potential
	Disulfide bond	157 ↔ 219		By similarity
	Disulfide bond	191 ↔ 201		By similarity
	Disulfide bond	234 ↔ 252		By similarity
	Disulfide bond	246 ↔ 261		By similarity
	Disulfide bond	264 ↔ 276		By similarity
	Disulfide bond	271 ↔ 289		By similarity
	Disulfide bond	283 ↔ 298		By similarity
	Disulfide bond	386 ↔ 402		By similarity
	Disulfide bond	487 ↔ 551		By similarity
	Disulfide bond	515 ↔ 530		By similarity
	Disulfide bond	541 ↔ 570		By similarity
Experimental info
	Sequence conflict	114	1	N → K in AAD32965. Ref.2
	Sequence conflict	236 – 252	17	NGKHI…GVNDC → MGSTFLRRKPATVSMTV in AAB00438. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q61129-1 [UniParc]. Last modified May 4, 2001. Version 2. Checksum: E2C1D43261C719E8	FASTA	603	67,234
	10 20 30 40 50 60 MKLAHLSLFL LALHLSSSRS PSASDLPQEE LVDQKCLLQK YTHRSCNKVF CQPWQRCIEG 70 80 90 100 110 120 TCICKLPYQC PRAGTPVCAM NGRSYPTYCH QKSFECLHPE IKFSHNGTCA AEGNFNVSLI 130 140 150 160 170 180 YGRTKTEGLV QVKLVDQDER MFICKNSWSM AEANVACVDL GFPLGVRDIQ GSFNISGNLH 190 200 210 220 230 240 INDTECLHVH CRGVETSLAE CAFTKRRTEL SNGLAGVVCY KQDADFPTSL SFQCVNGKHI 250 260 270 280 290 300 PQEKACNGVN DCGDQSDELC CKGCRGNASL CKSGVCIPDQ YKCNGEVDCI TGEDESRCEE 310 320 330 340 350 360 DRQQNIPKGL ARSAQGEAEI ETEETEMLTP GMDNERKRIK SLLPKLSCGV KRNTHTRRKR 370 380 390 400 410 420 VIGGKPANVG DYPWQVAIKD GQRITCGGIY IGGCWILTAA HCVRPSRAHS YQVWTALLDW 430 440 450 460 470 480 LKPNSQLGIQ TVKRVIVHEK YNGATFQNDI ALIEMKMHTG KKECELPNSV PACVPWSPYL 490 500 510 520 530 540 FQPNDRCIIS GWGRGKDNQK VYSLRWGEVD LIGNCSQFYP DRYYEKEMQC AGTRDGSIDA 550 560 570 580 590 600 CKGDSGGPLV CEDTNNVTYV WGIVSWGENC GKPEFPGVYT RVANYFDWIS YHVGRSLVSQ HNV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"cDNA cloning, sequencing and chromosomal assignment of the gene for mouse complement factor I (C3b/C4b inactivator): identification of a species specific divergent segment in factor I." Minta J.O., Wong M.J., Kozak C.A., Kunnath-Muglia L.M., Goldberger G. Mol. Immunol. 33:101-112(1996) [PubMed: 8604219] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: BALB/c.
[2]	"Cloning and characterization of the non-catalytic heavy chain of mouse complement factor I gene: structure comparison with the human homologue." Yun Y.-S., Goldberger G., Minta J.O. Biochem. Mol. Biol. Int. 47:493-500(1999) [PubMed: 10204086] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 114-334. Strain: 129/Sv. Tissue: Kidney.
[3]	"Proteome-wide characterization of N-glycosylation events by diagonal chromatography." Ghesquiere B., Van Damme J., Martens L., Vandekerckhove J., Gevaert K. J. Proteome Res. 5:2438-2447(2006) [PubMed: 16944957] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-116, MASS SPECTROMETRY. Tissue: Plasma.
[4]	"Enhanced analysis of the mouse plasma proteome using cysteine-containing tryptic glycopeptides." Bernhard O.K., Kapp E.A., Simpson R.J. J. Proteome Res. 6:987-995(2007) [PubMed: 17330941] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-514, MASS SPECTROMETRY. Tissue: Plasma.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U47810 mRNA. Translation: AAB00438.1. AF100565 , AF100559, AF100560, AF100561, AF100562, AF100563, AF100564 Genomic DNA. Translation: AAD32965.1.
IPI	IPI00320675.
UniGene	Mm.117180
3D structure databases
HSSP	HSSP built from PDB template 1RTF based on UniProtKB P00750.
ModBase	Search...
Proteomic databases
PRIDE	Q61129.
Genome annotation databases
Ensembl	ENSMUSG00000058952. Mus musculus. [Contig view]
Organism-specific databases
MGI	MGI:105937. Cfi.
Phylogenomic databases
HOGENOM	Q61129.
HOVERGEN	Q61129.
Enzyme and pathway databases
BRENDA	3.4.21.45. 244.
Gene expression databases
ArrayExpress	Q61129.
Bgee	Q61129.
CleanEx	MM_CFI.
GermOnline	ENSMUSG00000058952. Mus musculus.
Family and domain databases
InterPro	IPR003884. FacI_MAC. IPR002172. LDL_rcpt_classA_cys-rich. IPR018114. Peptidase_S1/S6_AS. IPR001254. Peptidase_S1_S6. IPR001314. Peptidase_S1A. IPR002350. Prot_inh_Kazal. IPR011497. Prot_Inh_Kazal_2. IPR001190. Srcr_rcpt. IPR017448. Srcr_rcpt-rel. [Graphical view]
Gene3D	G3DSA:4.10.400.10. LDL_rcpt_classA_cys-rich. 1 hit.
Pfam	PF07648. Kazal_2. 1 hit. PF00057. Ldl_recept_a. 2 hits. PF00530. SRCR. 1 hit. PF00089. Trypsin. 1 hit. [Graphical view]
PRINTS	PR00722. CHYMOTRYPSIN. PR00261. LDLRECEPTOR.
SMART	SM00057. FIMAC. 1 hit. SM00280. KAZAL. 1 hit. SM00192. LDLa. 2 hits. SM00202. SR. 1 hit. SM00020. Tryp_SPc. 1 hit. [Graphical view]
PROSITE	PS01209. LDLRA_1. 1 hit. PS50068. LDLRA_2. 2 hits. PS00420. SRCR_1. False negative. PS50287. SRCR_2. 1 hit. PS50240. TRYPSIN_DOM. 1 hit. PS00134. TRYPSIN_HIS. 1 hit. PS00135. TRYPSIN_SER. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
SOURCE	Search...

Entry information

Entry name

CFAI_MOUSE

Accession

Primary (citable) accession number: Q61129
Secondary accession number(s): Q9WU07

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 4, 2001
Last sequence update:	May 4, 2001
Last modified:	June 16, 2009
This is version 89 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)