SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q61129

- CFAI_MOUSE

UniProt

Q61129 - CFAI_MOUSE

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein
Complement factor I
Gene
Cfi, If
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Responsible for cleaving the alpha-chains of C4b and C3b in the presence of the cofactors C4-binding protein and factor H respectively.

Catalytic activityi

Inactivates complement subcomponents C3b, iC3b and C4b by proteolytic cleavage.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei401 – 4011Charge relay system By similarity
Active sitei449 – 4491Charge relay system By similarity
Active sitei545 – 5451Charge relay system By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Calcium bindingi244 – 25815 By similarity
Add
BLAST
Calcium bindingi281 – 29515 By similarity
Add
BLAST

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. scavenger receptor activity Source: InterPro
  3. serine-type endopeptidase activity Source: InterPro

GO - Biological processi

  1. complement activation, classical pathway Source: UniProtKB-KW
  2. innate immune response Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Keywords - Biological processi

Complement pathway, Immunity, Innate immunity

Keywords - Ligandi

Calcium, Metal-binding

Enzyme and pathway databases

BRENDAi3.4.21.45. 3474.
ReactomeiREACT_198562. Regulation of Complement cascade.

Protein family/group databases

MEROPSiS01.199.

Names & Taxonomyi

Protein namesi
Recommended name:
Complement factor I (EC:3.4.21.45)
Alternative name(s):
C3B/C4B inactivator
Cleaved into the following 2 chains:
Gene namesi
Name:Cfi
Synonyms:If
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 3

Organism-specific databases

MGIiMGI:105937. Cfi.

Subcellular locationi

GO - Cellular componenti

  1. extracellular space Source: UniProtKB-SubCell
  2. membrane Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1818 Reviewed prediction
Add
BLAST
Chaini19 – 603585Complement factor I
PRO_0000027571Add
BLAST
Chaini19 – 356338Complement factor I heavy chain
PRO_0000027572Add
BLAST
Chaini361 – 603243Complement factor I light chain
PRO_0000027573Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi36 ↔ 260 By similarity
Disulfide bondi46 ↔ 57 By similarity
Disulfide bondi51 ↔ 62 By similarity
Disulfide bondi64 ↔ 96 By similarity
Disulfide bondi70 ↔ 89 By similarity
Disulfide bondi78 ↔ 109 By similarity
Glycosylationi106 – 1061N-linked (GlcNAc...) Reviewed prediction
Glycosylationi116 – 1161N-linked (GlcNAc...)1 Publication
Disulfide bondi144 ↔ 186 By similarity
Disulfide bondi157 ↔ 219 By similarity
Glycosylationi174 – 1741N-linked (GlcNAc...) Reviewed prediction
Glycosylationi182 – 1821N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi191 ↔ 201 By similarity
Disulfide bondi234 ↔ 252 By similarity
Disulfide bondi264 ↔ 276 By similarity
Glycosylationi267 – 2671N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi271 ↔ 289 By similarity
Disulfide bondi283 ↔ 298 By similarity
Disulfide bondi348 ↔ 473Interchain (between heavy and light chains) By similarity
Disulfide bondi386 ↔ 402 By similarity
Disulfide bondi394 ↔ 464 By similarity
Disulfide bondi487 ↔ 551 By similarity
Glycosylationi514 – 5141N-linked (GlcNAc...)1 Publication
Disulfide bondi515 ↔ 530 By similarity
Disulfide bondi541 ↔ 570 By similarity
Glycosylationi556 – 5561N-linked (GlcNAc...) Reviewed prediction

Keywords - PTMi

Cleavage on pair of basic residues, Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiQ61129.
PaxDbiQ61129.
PRIDEiQ61129.

PTM databases

PhosphoSiteiQ61129.

Expressioni

Tissue specificityi

Plasma.

Gene expression databases

BgeeiQ61129.
CleanExiMM_CFI.
GenevestigatoriQ61129.

Interactioni

Subunit structurei

Heterodimer of a light and heavy chains; disulfide-linked. The fully processed and mature protein circulates as a zymogen, and is allosterically activated by substrate-induced remodeling of the active site By similarity.

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000077074.

Structurei

3D structure databases

ProteinModelPortaliQ61129.
SMRiQ61129. Positions 30-603.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini58 – 11154Kazal-like
Add
BLAST
Domaini117 – 217101SRCR
Add
BLAST
Domaini218 – 26245LDL-receptor class A 1
Add
BLAST
Domaini263 – 29937LDL-receptor class A 2
Add
BLAST
Domaini361 – 594234Peptidase S1
Add
BLAST

Sequence similaritiesi

Belongs to the peptidase S1 family.
Contains 1 Kazal-like domain.
Contains 1 SRCR domain.

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiCOG5640.
GeneTreeiENSGT00740000115494.
HOGENOMiHOG000060288.
HOVERGENiHBG005311.
InParanoidiB2RWX8.
KOiK01333.
OMAiLSCGVKN.
OrthoDBiEOG71G9TN.
TreeFamiTF330647.

Family and domain databases

Gene3Di3.10.250.10. 1 hit.
4.10.400.10. 2 hits.
InterProiIPR003884. FacI_MAC.
IPR002350. Kazal_dom.
IPR023415. LDLR_class-A_CS.
IPR002172. LDrepeatLR_classA_rpt.
IPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR001190. SRCR.
IPR017448. SRCR-like_dom.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamiPF07648. Kazal_2. 1 hit.
PF00057. Ldl_recept_a. 2 hits.
PF00530. SRCR. 1 hit.
PF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00057. FIMAC. 1 hit.
SM00280. KAZAL. 1 hit.
SM00192. LDLa. 2 hits.
SM00202. SR. 1 hit.
SM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
SSF56487. SSF56487. 1 hit.
SSF57424. SSF57424. 2 hits.
PROSITEiPS51465. KAZAL_2. 1 hit.
PS01209. LDLRA_1. 1 hit.
PS50068. LDLRA_2. 2 hits.
PS50287. SRCR_2. 1 hit.
PS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q61129-1 [UniParc]FASTAAdd to Basket

« Hide

MKLAHLSLFL LALHLSSSRS PSASDLPQEE LVDQKCLLQK YTHRSCNKVF    50
CQPWQRCIEG TCICKLPYQC PRAGTPVCAM NGRSYPTYCH QKSFECLHPE 100
IKFSHNGTCA AEGKFNVSLI YGRTKTEGLV QVKLVDQDER MFICKNSWSM 150
AEANVACVDL GFPLGVRDIQ GSFNISGNLH INDTECLHVH CRGVETSLAE 200
CAFTKRRTEL SNGLAGVVCY KQDADFPTSL SFQCVNGKHI PQEKACNGVN 250
DCGDQSDELC CKGCRGNASL CKSGVCIPDQ YKCNGEVDCI TGEDESRCEE 300
DRQQNIPKGL ARSAQGEAEI ETEETEMLTP GMDNERKRIK SLLPKLSCGV 350
KRNTHTRRKR VIGGKPANVG DYPWQVAIKD GQRITCGGIY IGGCWILTAA 400
HCVRPSRAHS YQVWTALLDW LKPNSQLGIQ TVKRVIVHEK YNGATFQNDI 450
ALIEMKMHTG KKECELPNSV PACVPWSPYL FQPNDRCIIS GWGRGKDNQK 500
VYSLRWGEVD LIGNCSQFYP DRYYEKEMQC AGTRDGSIDA CKGDSGGPLV 550
CEDINNVTYV WGIVSWGENC GKPEFPGVYT RVANYFDWIS YHVGRSLVSQ 600
HNV 603
Length:603
Mass (Da):67,261
Last modified:July 27, 2011 - v3
Checksum:iA9B90E1EC78AEE37
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti114 – 1141K → N in AAB00438. 1 Publication
Sequence conflicti236 – 25217NGKHI…GVNDC → MGSTFLRRKPATVSMTV in AAB00438. 1 Publication
Add
BLAST
Sequence conflicti554 – 5541I → T in AAB00438. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U47810 mRNA. Translation: AAB00438.1.
BC150751 mRNA. Translation: AAI50752.1.
AH007741 Genomic DNA. Translation: AAD32965.1.
CCDSiCCDS17835.1.
RefSeqiNP_031712.2. NM_007686.2.
XP_006501023.1. XM_006500960.1.
UniGeneiMm.117180.

Genome annotation databases

EnsembliENSMUST00000077918; ENSMUSP00000077074; ENSMUSG00000058952.
GeneIDi12630.
KEGGimmu:12630.
UCSCiuc008rin.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U47810 mRNA. Translation: AAB00438.1 .
BC150751 mRNA. Translation: AAI50752.1 .
AH007741 Genomic DNA. Translation: AAD32965.1 .
CCDSi CCDS17835.1.
RefSeqi NP_031712.2. NM_007686.2.
XP_006501023.1. XM_006500960.1.
UniGenei Mm.117180.

3D structure databases

ProteinModelPortali Q61129.
SMRi Q61129. Positions 30-603.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 10090.ENSMUSP00000077074.

Protein family/group databases

MEROPSi S01.199.

PTM databases

PhosphoSitei Q61129.

Proteomic databases

MaxQBi Q61129.
PaxDbi Q61129.
PRIDEi Q61129.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000077918 ; ENSMUSP00000077074 ; ENSMUSG00000058952 .
GeneIDi 12630.
KEGGi mmu:12630.
UCSCi uc008rin.1. mouse.

Organism-specific databases

CTDi 3426.
MGIi MGI:105937. Cfi.

Phylogenomic databases

eggNOGi COG5640.
GeneTreei ENSGT00740000115494.
HOGENOMi HOG000060288.
HOVERGENi HBG005311.
InParanoidi B2RWX8.
KOi K01333.
OMAi LSCGVKN.
OrthoDBi EOG71G9TN.
TreeFami TF330647.

Enzyme and pathway databases

BRENDAi 3.4.21.45. 3474.
Reactomei REACT_198562. Regulation of Complement cascade.

Miscellaneous databases

NextBioi 281812.
PROi Q61129.
SOURCEi Search...

Gene expression databases

Bgeei Q61129.
CleanExi MM_CFI.
Genevestigatori Q61129.

Family and domain databases

Gene3Di 3.10.250.10. 1 hit.
4.10.400.10. 2 hits.
InterProi IPR003884. FacI_MAC.
IPR002350. Kazal_dom.
IPR023415. LDLR_class-A_CS.
IPR002172. LDrepeatLR_classA_rpt.
IPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR001190. SRCR.
IPR017448. SRCR-like_dom.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view ]
Pfami PF07648. Kazal_2. 1 hit.
PF00057. Ldl_recept_a. 2 hits.
PF00530. SRCR. 1 hit.
PF00089. Trypsin. 1 hit.
[Graphical view ]
PRINTSi PR00722. CHYMOTRYPSIN.
SMARTi SM00057. FIMAC. 1 hit.
SM00280. KAZAL. 1 hit.
SM00192. LDLa. 2 hits.
SM00202. SR. 1 hit.
SM00020. Tryp_SPc. 1 hit.
[Graphical view ]
SUPFAMi SSF50494. SSF50494. 1 hit.
SSF56487. SSF56487. 1 hit.
SSF57424. SSF57424. 2 hits.
PROSITEi PS51465. KAZAL_2. 1 hit.
PS01209. LDLRA_1. 1 hit.
PS50068. LDLRA_2. 2 hits.
PS50287. SRCR_2. 1 hit.
PS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "cDNA cloning, sequencing and chromosomal assignment of the gene for mouse complement factor I (C3b/C4b inactivator): identification of a species specific divergent segment in factor I."
    Minta J.O., Wong M.J., Kozak C.A., Kunnath-Muglia L.M., Goldberger G.
    Mol. Immunol. 33:101-112(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: BALB/c.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain.
  3. "Cloning and characterization of the non-catalytic heavy chain of mouse complement factor I gene: structure comparison with the human homologue."
    Yun Y.-S., Goldberger G., Minta J.O.
    Biochem. Mol. Biol. Int. 47:493-500(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 114-334.
    Strain: 129/Sv.
    Tissue: Kidney.
  4. "Proteome-wide characterization of N-glycosylation events by diagonal chromatography."
    Ghesquiere B., Van Damme J., Martens L., Vandekerckhove J., Gevaert K.
    J. Proteome Res. 5:2438-2447(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-116.
    Strain: C57BL/6.
    Tissue: Plasma.
  5. "Enhanced analysis of the mouse plasma proteome using cysteine-containing tryptic glycopeptides."
    Bernhard O.K., Kapp E.A., Simpson R.J.
    J. Proteome Res. 6:987-995(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-514.
    Strain: C57BL/6.
    Tissue: Plasma.

Entry informationi

Entry nameiCFAI_MOUSE
AccessioniPrimary (citable) accession number: Q61129
Secondary accession number(s): B2RWX8, Q9WU07
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 4, 2001
Last sequence update: July 27, 2011
Last modified: September 3, 2014
This is version 137 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi