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Q61129

- CFAI_MOUSE

UniProt

Q61129 - CFAI_MOUSE

Protein

Complement factor I

Gene

Cfi

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 138 (01 Oct 2014)
      Sequence version 3 (27 Jul 2011)
      Previous versions | rss
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    Functioni

    Responsible for cleaving the alpha-chains of C4b and C3b in the presence of the cofactors C4-binding protein and factor H respectively.

    Catalytic activityi

    Inactivates complement subcomponents C3b, iC3b and C4b by proteolytic cleavage.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei401 – 4011Charge relay systemBy similarity
    Active sitei449 – 4491Charge relay systemBy similarity
    Active sitei545 – 5451Charge relay systemBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Calcium bindingi244 – 25815By similarityAdd
    BLAST
    Calcium bindingi281 – 29515By similarityAdd
    BLAST

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. scavenger receptor activity Source: InterPro
    3. serine-type endopeptidase activity Source: InterPro

    GO - Biological processi

    1. complement activation, classical pathway Source: UniProtKB-KW
    2. innate immune response Source: UniProtKB-KW

    Keywords - Molecular functioni

    Hydrolase, Protease, Serine protease

    Keywords - Biological processi

    Complement pathway, Immunity, Innate immunity

    Keywords - Ligandi

    Calcium, Metal-binding

    Enzyme and pathway databases

    BRENDAi3.4.21.45. 3474.
    ReactomeiREACT_198562. Regulation of Complement cascade.

    Protein family/group databases

    MEROPSiS01.199.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Complement factor I (EC:3.4.21.45)
    Alternative name(s):
    C3B/C4B inactivator
    Cleaved into the following 2 chains:
    Gene namesi
    Name:Cfi
    Synonyms:If
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 3

    Organism-specific databases

    MGIiMGI:105937. Cfi.

    Subcellular locationi

    GO - Cellular componenti

    1. extracellular space Source: UniProtKB-SubCell
    2. membrane Source: InterPro

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1818Sequence AnalysisAdd
    BLAST
    Chaini19 – 603585Complement factor IPRO_0000027571Add
    BLAST
    Chaini19 – 356338Complement factor I heavy chainPRO_0000027572Add
    BLAST
    Chaini361 – 603243Complement factor I light chainPRO_0000027573Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi36 ↔ 260By similarity
    Disulfide bondi46 ↔ 57By similarity
    Disulfide bondi51 ↔ 62By similarity
    Disulfide bondi64 ↔ 96By similarity
    Disulfide bondi70 ↔ 89By similarity
    Disulfide bondi78 ↔ 109By similarity
    Glycosylationi106 – 1061N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi116 – 1161N-linked (GlcNAc...)1 Publication
    Disulfide bondi144 ↔ 186By similarity
    Disulfide bondi157 ↔ 219By similarity
    Glycosylationi174 – 1741N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi182 – 1821N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi191 ↔ 201By similarity
    Disulfide bondi234 ↔ 252By similarity
    Disulfide bondi264 ↔ 276By similarity
    Glycosylationi267 – 2671N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi271 ↔ 289By similarity
    Disulfide bondi283 ↔ 298By similarity
    Disulfide bondi348 ↔ 473Interchain (between heavy and light chains)PROSITE-ProRule annotation
    Disulfide bondi386 ↔ 402By similarity
    Disulfide bondi394 ↔ 464By similarity
    Disulfide bondi487 ↔ 551By similarity
    Glycosylationi514 – 5141N-linked (GlcNAc...)1 Publication
    Disulfide bondi515 ↔ 530By similarity
    Disulfide bondi541 ↔ 570By similarity
    Glycosylationi556 – 5561N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Cleavage on pair of basic residues, Disulfide bond, Glycoprotein

    Proteomic databases

    MaxQBiQ61129.
    PaxDbiQ61129.
    PRIDEiQ61129.

    PTM databases

    PhosphoSiteiQ61129.

    Expressioni

    Tissue specificityi

    Plasma.

    Gene expression databases

    BgeeiQ61129.
    CleanExiMM_CFI.
    GenevestigatoriQ61129.

    Interactioni

    Subunit structurei

    Heterodimer of a light and heavy chains; disulfide-linked. The fully processed and mature protein circulates as a zymogen, and is allosterically activated by substrate-induced remodeling of the active site By similarity.By similarity

    Protein-protein interaction databases

    STRINGi10090.ENSMUSP00000077074.

    Structurei

    3D structure databases

    ProteinModelPortaliQ61129.
    SMRiQ61129. Positions 30-603.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini58 – 11154Kazal-likePROSITE-ProRule annotationAdd
    BLAST
    Domaini117 – 217101SRCRPROSITE-ProRule annotationAdd
    BLAST
    Domaini218 – 26245LDL-receptor class A 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini263 – 29937LDL-receptor class A 2PROSITE-ProRule annotationAdd
    BLAST
    Domaini361 – 594234Peptidase S1PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the peptidase S1 family.PROSITE-ProRule annotation
    Contains 1 Kazal-like domain.PROSITE-ProRule annotation
    Contains 2 LDL-receptor class A domains.PROSITE-ProRule annotation
    Contains 1 peptidase S1 domain.PROSITE-ProRule annotation
    Contains 1 SRCR domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat, Signal

    Phylogenomic databases

    eggNOGiCOG5640.
    GeneTreeiENSGT00740000115494.
    HOGENOMiHOG000060288.
    HOVERGENiHBG005311.
    InParanoidiB2RWX8.
    KOiK01333.
    OMAiLSCGVKN.
    OrthoDBiEOG71G9TN.
    TreeFamiTF330647.

    Family and domain databases

    Gene3Di3.10.250.10. 1 hit.
    4.10.400.10. 2 hits.
    InterProiIPR003884. FacI_MAC.
    IPR002350. Kazal_dom.
    IPR023415. LDLR_class-A_CS.
    IPR002172. LDrepeatLR_classA_rpt.
    IPR001254. Peptidase_S1.
    IPR018114. Peptidase_S1_AS.
    IPR001314. Peptidase_S1A.
    IPR001190. SRCR.
    IPR017448. SRCR-like_dom.
    IPR009003. Trypsin-like_Pept_dom.
    [Graphical view]
    PfamiPF07648. Kazal_2. 1 hit.
    PF00057. Ldl_recept_a. 2 hits.
    PF00530. SRCR. 1 hit.
    PF00089. Trypsin. 1 hit.
    [Graphical view]
    PRINTSiPR00722. CHYMOTRYPSIN.
    SMARTiSM00057. FIMAC. 1 hit.
    SM00280. KAZAL. 1 hit.
    SM00192. LDLa. 2 hits.
    SM00202. SR. 1 hit.
    SM00020. Tryp_SPc. 1 hit.
    [Graphical view]
    SUPFAMiSSF50494. SSF50494. 1 hit.
    SSF56487. SSF56487. 1 hit.
    SSF57424. SSF57424. 2 hits.
    PROSITEiPS51465. KAZAL_2. 1 hit.
    PS01209. LDLRA_1. 1 hit.
    PS50068. LDLRA_2. 2 hits.
    PS50287. SRCR_2. 1 hit.
    PS50240. TRYPSIN_DOM. 1 hit.
    PS00134. TRYPSIN_HIS. 1 hit.
    PS00135. TRYPSIN_SER. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q61129-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKLAHLSLFL LALHLSSSRS PSASDLPQEE LVDQKCLLQK YTHRSCNKVF    50
    CQPWQRCIEG TCICKLPYQC PRAGTPVCAM NGRSYPTYCH QKSFECLHPE 100
    IKFSHNGTCA AEGKFNVSLI YGRTKTEGLV QVKLVDQDER MFICKNSWSM 150
    AEANVACVDL GFPLGVRDIQ GSFNISGNLH INDTECLHVH CRGVETSLAE 200
    CAFTKRRTEL SNGLAGVVCY KQDADFPTSL SFQCVNGKHI PQEKACNGVN 250
    DCGDQSDELC CKGCRGNASL CKSGVCIPDQ YKCNGEVDCI TGEDESRCEE 300
    DRQQNIPKGL ARSAQGEAEI ETEETEMLTP GMDNERKRIK SLLPKLSCGV 350
    KRNTHTRRKR VIGGKPANVG DYPWQVAIKD GQRITCGGIY IGGCWILTAA 400
    HCVRPSRAHS YQVWTALLDW LKPNSQLGIQ TVKRVIVHEK YNGATFQNDI 450
    ALIEMKMHTG KKECELPNSV PACVPWSPYL FQPNDRCIIS GWGRGKDNQK 500
    VYSLRWGEVD LIGNCSQFYP DRYYEKEMQC AGTRDGSIDA CKGDSGGPLV 550
    CEDINNVTYV WGIVSWGENC GKPEFPGVYT RVANYFDWIS YHVGRSLVSQ 600
    HNV 603
    Length:603
    Mass (Da):67,261
    Last modified:July 27, 2011 - v3
    Checksum:iA9B90E1EC78AEE37
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti114 – 1141K → N in AAB00438. (PubMed:8604219)Curated
    Sequence conflicti236 – 25217NGKHI…GVNDC → MGSTFLRRKPATVSMTV in AAB00438. (PubMed:8604219)CuratedAdd
    BLAST
    Sequence conflicti554 – 5541I → T in AAB00438. (PubMed:8604219)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U47810 mRNA. Translation: AAB00438.1.
    BC150751 mRNA. Translation: AAI50752.1.
    AH007741 Genomic DNA. Translation: AAD32965.1.
    CCDSiCCDS17835.1.
    RefSeqiNP_031712.2. NM_007686.2.
    XP_006501023.1. XM_006500960.1.
    UniGeneiMm.117180.

    Genome annotation databases

    EnsembliENSMUST00000077918; ENSMUSP00000077074; ENSMUSG00000058952.
    GeneIDi12630.
    KEGGimmu:12630.
    UCSCiuc008rin.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U47810 mRNA. Translation: AAB00438.1 .
    BC150751 mRNA. Translation: AAI50752.1 .
    AH007741 Genomic DNA. Translation: AAD32965.1 .
    CCDSi CCDS17835.1.
    RefSeqi NP_031712.2. NM_007686.2.
    XP_006501023.1. XM_006500960.1.
    UniGenei Mm.117180.

    3D structure databases

    ProteinModelPortali Q61129.
    SMRi Q61129. Positions 30-603.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10090.ENSMUSP00000077074.

    Protein family/group databases

    MEROPSi S01.199.

    PTM databases

    PhosphoSitei Q61129.

    Proteomic databases

    MaxQBi Q61129.
    PaxDbi Q61129.
    PRIDEi Q61129.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000077918 ; ENSMUSP00000077074 ; ENSMUSG00000058952 .
    GeneIDi 12630.
    KEGGi mmu:12630.
    UCSCi uc008rin.1. mouse.

    Organism-specific databases

    CTDi 3426.
    MGIi MGI:105937. Cfi.

    Phylogenomic databases

    eggNOGi COG5640.
    GeneTreei ENSGT00740000115494.
    HOGENOMi HOG000060288.
    HOVERGENi HBG005311.
    InParanoidi B2RWX8.
    KOi K01333.
    OMAi LSCGVKN.
    OrthoDBi EOG71G9TN.
    TreeFami TF330647.

    Enzyme and pathway databases

    BRENDAi 3.4.21.45. 3474.
    Reactomei REACT_198562. Regulation of Complement cascade.

    Miscellaneous databases

    NextBioi 281812.
    PROi Q61129.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q61129.
    CleanExi MM_CFI.
    Genevestigatori Q61129.

    Family and domain databases

    Gene3Di 3.10.250.10. 1 hit.
    4.10.400.10. 2 hits.
    InterProi IPR003884. FacI_MAC.
    IPR002350. Kazal_dom.
    IPR023415. LDLR_class-A_CS.
    IPR002172. LDrepeatLR_classA_rpt.
    IPR001254. Peptidase_S1.
    IPR018114. Peptidase_S1_AS.
    IPR001314. Peptidase_S1A.
    IPR001190. SRCR.
    IPR017448. SRCR-like_dom.
    IPR009003. Trypsin-like_Pept_dom.
    [Graphical view ]
    Pfami PF07648. Kazal_2. 1 hit.
    PF00057. Ldl_recept_a. 2 hits.
    PF00530. SRCR. 1 hit.
    PF00089. Trypsin. 1 hit.
    [Graphical view ]
    PRINTSi PR00722. CHYMOTRYPSIN.
    SMARTi SM00057. FIMAC. 1 hit.
    SM00280. KAZAL. 1 hit.
    SM00192. LDLa. 2 hits.
    SM00202. SR. 1 hit.
    SM00020. Tryp_SPc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50494. SSF50494. 1 hit.
    SSF56487. SSF56487. 1 hit.
    SSF57424. SSF57424. 2 hits.
    PROSITEi PS51465. KAZAL_2. 1 hit.
    PS01209. LDLRA_1. 1 hit.
    PS50068. LDLRA_2. 2 hits.
    PS50287. SRCR_2. 1 hit.
    PS50240. TRYPSIN_DOM. 1 hit.
    PS00134. TRYPSIN_HIS. 1 hit.
    PS00135. TRYPSIN_SER. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "cDNA cloning, sequencing and chromosomal assignment of the gene for mouse complement factor I (C3b/C4b inactivator): identification of a species specific divergent segment in factor I."
      Minta J.O., Wong M.J., Kozak C.A., Kunnath-Muglia L.M., Goldberger G.
      Mol. Immunol. 33:101-112(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: BALB/c.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Brain.
    3. "Cloning and characterization of the non-catalytic heavy chain of mouse complement factor I gene: structure comparison with the human homologue."
      Yun Y.-S., Goldberger G., Minta J.O.
      Biochem. Mol. Biol. Int. 47:493-500(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 114-334.
      Strain: 129/Sv.
      Tissue: Kidney.
    4. "Proteome-wide characterization of N-glycosylation events by diagonal chromatography."
      Ghesquiere B., Van Damme J., Martens L., Vandekerckhove J., Gevaert K.
      J. Proteome Res. 5:2438-2447(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-116.
      Strain: C57BL/6.
      Tissue: Plasma.
    5. "Enhanced analysis of the mouse plasma proteome using cysteine-containing tryptic glycopeptides."
      Bernhard O.K., Kapp E.A., Simpson R.J.
      J. Proteome Res. 6:987-995(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-514.
      Strain: C57BL/6.
      Tissue: Plasma.

    Entry informationi

    Entry nameiCFAI_MOUSE
    AccessioniPrimary (citable) accession number: Q61129
    Secondary accession number(s): B2RWX8, Q9WU07
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 4, 2001
    Last sequence update: July 27, 2011
    Last modified: October 1, 2014
    This is version 138 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. Peptidase families
      Classification of peptidase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3