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Protein

Complement factor I

Gene

Cfi

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Responsible for cleaving the alpha-chains of C4b and C3b in the presence of the cofactors C4-binding protein and factor H respectively.

Catalytic activityi

Inactivates complement subcomponents C3b, iC3b and C4b by proteolytic cleavage.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei401Charge relay systemBy similarity1
Active sitei449Charge relay systemBy similarity1
Active sitei545Charge relay systemBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Calcium bindingi244 – 258By similarityAdd BLAST15
Calcium bindingi281 – 295By similarityAdd BLAST15

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Protease, Serine protease
Biological processComplement pathway, Immunity, Innate immunity
LigandCalcium, Metal-binding

Enzyme and pathway databases

BRENDAi3.4.21.45. 3474.
ReactomeiR-MMU-977606. Regulation of Complement cascade.

Protein family/group databases

MEROPSiS01.199.

Names & Taxonomyi

Protein namesi
Recommended name:
Complement factor I (EC:3.4.21.45)
Alternative name(s):
C3B/C4B inactivator
Cleaved into the following 2 chains:
Gene namesi
Name:Cfi
Synonyms:If
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 3

Organism-specific databases

MGIiMGI:105937. Cfi.

Subcellular locationi

GO - Cellular componenti

  • extracellular exosome Source: MGI
  • extracellular space Source: BHF-UCL
  • membrane Source: InterPro

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 18Sequence analysisAdd BLAST18
ChainiPRO_000002757119 – 603Complement factor IAdd BLAST585
ChainiPRO_000002757219 – 356Complement factor I heavy chainAdd BLAST338
ChainiPRO_0000027573361 – 603Complement factor I light chainAdd BLAST243

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi36 ↔ 260By similarity
Disulfide bondi46 ↔ 57By similarity
Disulfide bondi51 ↔ 62By similarity
Disulfide bondi64 ↔ 96By similarity
Disulfide bondi70 ↔ 89By similarity
Disulfide bondi78 ↔ 109By similarity
Glycosylationi106N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi116N-linked (GlcNAc...) asparagine1 Publication1
Disulfide bondi144 ↔ 186By similarity
Disulfide bondi157 ↔ 219By similarity
Glycosylationi174N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi182N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi191 ↔ 201By similarity
Disulfide bondi234 ↔ 252By similarity
Disulfide bondi264 ↔ 276By similarity
Glycosylationi267N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi271 ↔ 289By similarity
Disulfide bondi283 ↔ 298By similarity
Disulfide bondi348 ↔ 473Interchain (between heavy and light chains)PROSITE-ProRule annotation
Disulfide bondi386 ↔ 402By similarity
Disulfide bondi394 ↔ 464By similarity
Disulfide bondi487 ↔ 551By similarity
Glycosylationi514N-linked (GlcNAc...) asparagine1 Publication1
Disulfide bondi515 ↔ 530By similarity
Disulfide bondi541 ↔ 570By similarity
Glycosylationi556N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Cleavage on pair of basic residues, Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiQ61129.
PaxDbiQ61129.
PeptideAtlasiQ61129.
PRIDEiQ61129.

PTM databases

iPTMnetiQ61129.
PhosphoSitePlusiQ61129.

Expressioni

Tissue specificityi

Plasma.

Gene expression databases

BgeeiENSMUSG00000058952.
CleanExiMM_CFI.
ExpressionAtlasiQ61129. baseline and differential.
GenevisibleiQ61129. MM.

Interactioni

Subunit structurei

Heterodimer of a light and heavy chains; disulfide-linked. The fully processed and mature protein circulates as a zymogen, and is allosterically activated by substrate-induced remodeling of the active site (By similarity).By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000077074.

Structurei

3D structure databases

ProteinModelPortaliQ61129.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini58 – 111Kazal-likePROSITE-ProRule annotationAdd BLAST54
Domaini117 – 217SRCRPROSITE-ProRule annotationAdd BLAST101
Domaini218 – 262LDL-receptor class A 1PROSITE-ProRule annotationAdd BLAST45
Domaini263 – 299LDL-receptor class A 2PROSITE-ProRule annotationAdd BLAST37
Domaini361 – 594Peptidase S1PROSITE-ProRule annotationAdd BLAST234

Sequence similaritiesi

Belongs to the peptidase S1 family.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiKOG3627. Eukaryota.
COG5640. LUCA.
GeneTreeiENSGT00890000139364.
HOGENOMiHOG000060288.
HOVERGENiHBG005311.
InParanoidiQ61129.
KOiK01333.
OMAiWGREKDN.
OrthoDBiEOG091G03T2.
TreeFamiTF330647.

Family and domain databases

CDDicd00190. Tryp_SPc. 1 hit.
Gene3Di3.10.250.10. 1 hit.
InterProiView protein in InterPro
IPR003884. FacI_MAC.
IPR002350. Kazal_dom.
IPR023415. LDLR_class-A_CS.
IPR002172. LDrepeatLR_classA_rpt.
IPR009003. Peptidase_S1_PA.
IPR001314. Peptidase_S1A.
IPR001190. SRCR.
IPR017448. SRCR-like_dom.
IPR001254. Trypsin_dom.
IPR018114. TRYPSIN_HIS.
IPR033116. TRYPSIN_SER.
PfamiView protein in Pfam
PF07648. Kazal_2. 1 hit.
PF00057. Ldl_recept_a. 2 hits.
PF00530. SRCR. 1 hit.
PF00089. Trypsin. 1 hit.
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiView protein in SMART
SM00057. FIMAC. 1 hit.
SM00192. LDLa. 2 hits.
SM00202. SR. 1 hit.
SM00020. Tryp_SPc. 1 hit.
SUPFAMiSSF100895. SSF100895. 1 hit.
SSF50494. SSF50494. 1 hit.
SSF56487. SSF56487. 1 hit.
SSF57424. SSF57424. 2 hits.
PROSITEiView protein in PROSITE
PS51465. KAZAL_2. 1 hit.
PS01209. LDLRA_1. 1 hit.
PS50068. LDLRA_2. 2 hits.
PS50287. SRCR_2. 1 hit.
PS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q61129-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKLAHLSLFL LALHLSSSRS PSASDLPQEE LVDQKCLLQK YTHRSCNKVF
60 70 80 90 100
CQPWQRCIEG TCICKLPYQC PRAGTPVCAM NGRSYPTYCH QKSFECLHPE
110 120 130 140 150
IKFSHNGTCA AEGKFNVSLI YGRTKTEGLV QVKLVDQDER MFICKNSWSM
160 170 180 190 200
AEANVACVDL GFPLGVRDIQ GSFNISGNLH INDTECLHVH CRGVETSLAE
210 220 230 240 250
CAFTKRRTEL SNGLAGVVCY KQDADFPTSL SFQCVNGKHI PQEKACNGVN
260 270 280 290 300
DCGDQSDELC CKGCRGNASL CKSGVCIPDQ YKCNGEVDCI TGEDESRCEE
310 320 330 340 350
DRQQNIPKGL ARSAQGEAEI ETEETEMLTP GMDNERKRIK SLLPKLSCGV
360 370 380 390 400
KRNTHTRRKR VIGGKPANVG DYPWQVAIKD GQRITCGGIY IGGCWILTAA
410 420 430 440 450
HCVRPSRAHS YQVWTALLDW LKPNSQLGIQ TVKRVIVHEK YNGATFQNDI
460 470 480 490 500
ALIEMKMHTG KKECELPNSV PACVPWSPYL FQPNDRCIIS GWGRGKDNQK
510 520 530 540 550
VYSLRWGEVD LIGNCSQFYP DRYYEKEMQC AGTRDGSIDA CKGDSGGPLV
560 570 580 590 600
CEDINNVTYV WGIVSWGENC GKPEFPGVYT RVANYFDWIS YHVGRSLVSQ

HNV
Length:603
Mass (Da):67,261
Last modified:July 27, 2011 - v3
Checksum:iA9B90E1EC78AEE37
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti114K → N in AAB00438 (PubMed:8604219).Curated1
Sequence conflicti236 – 252NGKHI…GVNDC → MGSTFLRRKPATVSMTV in AAB00438 (PubMed:8604219).CuratedAdd BLAST17
Sequence conflicti554I → T in AAB00438 (PubMed:8604219).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U47810 mRNA. Translation: AAB00438.1.
BC150751 mRNA. Translation: AAI50752.1.
AH007741 Genomic DNA. Translation: AAD32965.1.
CCDSiCCDS17835.1.
RefSeqiNP_031712.2. NM_007686.3.
UniGeneiMm.117180.

Genome annotation databases

EnsembliENSMUST00000077918; ENSMUSP00000077074; ENSMUSG00000058952.
GeneIDi12630.
KEGGimmu:12630.
UCSCiuc008rin.1. mouse.

Similar proteinsi

Entry informationi

Entry nameiCFAI_MOUSE
AccessioniPrimary (citable) accession number: Q61129
Secondary accession number(s): B2RWX8, Q9WU07
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 4, 2001
Last sequence update: July 27, 2011
Last modified: August 30, 2017
This is version 165 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families