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Protein

Complement factor I

Gene

Cfi

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Responsible for cleaving the alpha-chains of C4b and C3b in the presence of the cofactors C4-binding protein and factor H respectively.

Catalytic activityi

Inactivates complement subcomponents C3b, iC3b and C4b by proteolytic cleavage.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei401Charge relay systemBy similarity1
Active sitei449Charge relay systemBy similarity1
Active sitei545Charge relay systemBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Calcium bindingi244 – 258By similarityAdd BLAST15
Calcium bindingi281 – 295By similarityAdd BLAST15

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Protease, Serine protease
Biological processComplement pathway, Immunity, Innate immunity
LigandCalcium, Metal-binding

Enzyme and pathway databases

BRENDAi3.4.21.45 3474
ReactomeiR-MMU-977606 Regulation of Complement cascade

Protein family/group databases

MEROPSiS01.199

Names & Taxonomyi

Protein namesi
Recommended name:
Complement factor I (EC:3.4.21.45)
Alternative name(s):
C3B/C4B inactivator
Cleaved into the following 2 chains:
Gene namesi
Name:Cfi
Synonyms:If
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 3

Organism-specific databases

MGIiMGI:105937 Cfi

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 18Sequence analysisAdd BLAST18
ChainiPRO_000002757119 – 603Complement factor IAdd BLAST585
ChainiPRO_000002757219 – 356Complement factor I heavy chainAdd BLAST338
ChainiPRO_0000027573361 – 603Complement factor I light chainAdd BLAST243

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi36 ↔ 260By similarity
Disulfide bondi46 ↔ 57By similarity
Disulfide bondi51 ↔ 62By similarity
Disulfide bondi64 ↔ 96By similarity
Disulfide bondi70 ↔ 89By similarity
Disulfide bondi78 ↔ 109By similarity
Glycosylationi106N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi116N-linked (GlcNAc...) asparagine1 Publication1
Disulfide bondi144 ↔ 186By similarity
Disulfide bondi157 ↔ 219By similarity
Glycosylationi174N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi182N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi191 ↔ 201By similarity
Disulfide bondi234 ↔ 252By similarity
Disulfide bondi264 ↔ 276By similarity
Glycosylationi267N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi271 ↔ 289By similarity
Disulfide bondi283 ↔ 298By similarity
Disulfide bondi348 ↔ 473Interchain (between heavy and light chains)PROSITE-ProRule annotation
Disulfide bondi386 ↔ 402By similarity
Disulfide bondi394 ↔ 464By similarity
Disulfide bondi487 ↔ 551By similarity
Glycosylationi514N-linked (GlcNAc...) asparagine1 Publication1
Disulfide bondi515 ↔ 530By similarity
Disulfide bondi541 ↔ 570By similarity
Glycosylationi556N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Cleavage on pair of basic residues, Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiQ61129
PaxDbiQ61129
PeptideAtlasiQ61129
PRIDEiQ61129

PTM databases

iPTMnetiQ61129
PhosphoSitePlusiQ61129

Expressioni

Tissue specificityi

Plasma.

Gene expression databases

BgeeiENSMUSG00000058952
CleanExiMM_CFI
ExpressionAtlasiQ61129 baseline and differential
GenevisibleiQ61129 MM

Interactioni

Subunit structurei

Heterodimer of a light and heavy chains; disulfide-linked. The fully processed and mature protein circulates as a zymogen, and is allosterically activated by substrate-induced remodeling of the active site (By similarity).By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000077074

Structurei

3D structure databases

ProteinModelPortaliQ61129
SMRiQ61129
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini58 – 111Kazal-likePROSITE-ProRule annotationAdd BLAST54
Domaini117 – 217SRCRPROSITE-ProRule annotationAdd BLAST101
Domaini218 – 262LDL-receptor class A 1PROSITE-ProRule annotationAdd BLAST45
Domaini263 – 299LDL-receptor class A 2PROSITE-ProRule annotationAdd BLAST37
Domaini361 – 594Peptidase S1PROSITE-ProRule annotationAdd BLAST234

Sequence similaritiesi

Belongs to the peptidase S1 family.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiKOG3627 Eukaryota
COG5640 LUCA
GeneTreeiENSGT00900000140784
HOGENOMiHOG000060288
HOVERGENiHBG005311
InParanoidiQ61129
KOiK01333
OMAiWGREKDN
OrthoDBiEOG091G03T2
TreeFamiTF330647

Family and domain databases

CDDicd00112 LDLa, 2 hits
cd00190 Tryp_SPc, 1 hit
Gene3Di3.10.250.10, 1 hit
InterProiView protein in InterPro
IPR003884 FacI_MAC
IPR002350 Kazal_dom
IPR036058 Kazal_dom_sf
IPR036055 LDL_receptor-like_sf
IPR023415 LDLR_class-A_CS
IPR002172 LDrepeatLR_classA_rpt
IPR009003 Peptidase_S1_PA
IPR001314 Peptidase_S1A
IPR001190 SRCR
IPR017448 SRCR-like_dom
IPR036772 SRCR-like_dom_sf
IPR001254 Trypsin_dom
IPR018114 TRYPSIN_HIS
IPR033116 TRYPSIN_SER
PfamiView protein in Pfam
PF07648 Kazal_2, 1 hit
PF00057 Ldl_recept_a, 2 hits
PF00530 SRCR, 1 hit
PF00089 Trypsin, 1 hit
PRINTSiPR00722 CHYMOTRYPSIN
SMARTiView protein in SMART
SM00057 FIMAC, 1 hit
SM00192 LDLa, 2 hits
SM00202 SR, 1 hit
SM00020 Tryp_SPc, 1 hit
SUPFAMiSSF100895 SSF100895, 1 hit
SSF50494 SSF50494, 1 hit
SSF56487 SSF56487, 1 hit
SSF57424 SSF57424, 2 hits
PROSITEiView protein in PROSITE
PS51465 KAZAL_2, 1 hit
PS01209 LDLRA_1, 1 hit
PS50068 LDLRA_2, 2 hits
PS50287 SRCR_2, 1 hit
PS50240 TRYPSIN_DOM, 1 hit
PS00134 TRYPSIN_HIS, 1 hit
PS00135 TRYPSIN_SER, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q61129-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKLAHLSLFL LALHLSSSRS PSASDLPQEE LVDQKCLLQK YTHRSCNKVF
60 70 80 90 100
CQPWQRCIEG TCICKLPYQC PRAGTPVCAM NGRSYPTYCH QKSFECLHPE
110 120 130 140 150
IKFSHNGTCA AEGKFNVSLI YGRTKTEGLV QVKLVDQDER MFICKNSWSM
160 170 180 190 200
AEANVACVDL GFPLGVRDIQ GSFNISGNLH INDTECLHVH CRGVETSLAE
210 220 230 240 250
CAFTKRRTEL SNGLAGVVCY KQDADFPTSL SFQCVNGKHI PQEKACNGVN
260 270 280 290 300
DCGDQSDELC CKGCRGNASL CKSGVCIPDQ YKCNGEVDCI TGEDESRCEE
310 320 330 340 350
DRQQNIPKGL ARSAQGEAEI ETEETEMLTP GMDNERKRIK SLLPKLSCGV
360 370 380 390 400
KRNTHTRRKR VIGGKPANVG DYPWQVAIKD GQRITCGGIY IGGCWILTAA
410 420 430 440 450
HCVRPSRAHS YQVWTALLDW LKPNSQLGIQ TVKRVIVHEK YNGATFQNDI
460 470 480 490 500
ALIEMKMHTG KKECELPNSV PACVPWSPYL FQPNDRCIIS GWGRGKDNQK
510 520 530 540 550
VYSLRWGEVD LIGNCSQFYP DRYYEKEMQC AGTRDGSIDA CKGDSGGPLV
560 570 580 590 600
CEDINNVTYV WGIVSWGENC GKPEFPGVYT RVANYFDWIS YHVGRSLVSQ

HNV
Length:603
Mass (Da):67,261
Last modified:July 27, 2011 - v3
Checksum:iA9B90E1EC78AEE37
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti114K → N in AAB00438 (PubMed:8604219).Curated1
Sequence conflicti236 – 252NGKHI…GVNDC → MGSTFLRRKPATVSMTV in AAB00438 (PubMed:8604219).CuratedAdd BLAST17
Sequence conflicti554I → T in AAB00438 (PubMed:8604219).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U47810 mRNA Translation: AAB00438.1
BC150751 mRNA Translation: AAI50752.1
AH007741 Genomic DNA Translation: AAD32965.1
CCDSiCCDS17835.1
RefSeqiNP_031712.2, NM_007686.3
UniGeneiMm.117180

Genome annotation databases

EnsembliENSMUST00000077918; ENSMUSP00000077074; ENSMUSG00000058952
GeneIDi12630
KEGGimmu:12630
UCSCiuc008rin.1 mouse

Similar proteinsi

Entry informationi

Entry nameiCFAI_MOUSE
AccessioniPrimary (citable) accession number: Q61129
Secondary accession number(s): B2RWX8, Q9WU07
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 4, 2001
Last sequence update: July 27, 2011
Last modified: May 23, 2018
This is version 168 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

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