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Q61129

- CFAI_MOUSE

UniProt

Q61129 - CFAI_MOUSE

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Protein

Complement factor I

Gene

Cfi

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Responsible for cleaving the alpha-chains of C4b and C3b in the presence of the cofactors C4-binding protein and factor H respectively.

Catalytic activityi

Inactivates complement subcomponents C3b, iC3b and C4b by proteolytic cleavage.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei401 – 4011Charge relay systemBy similarity
Active sitei449 – 4491Charge relay systemBy similarity
Active sitei545 – 5451Charge relay systemBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Calcium bindingi244 – 25815By similarityAdd
BLAST
Calcium bindingi281 – 29515By similarityAdd
BLAST

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. scavenger receptor activity Source: InterPro
  3. serine-type endopeptidase activity Source: InterPro

GO - Biological processi

  1. complement activation, classical pathway Source: UniProtKB-KW
  2. innate immune response Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Keywords - Biological processi

Complement pathway, Immunity, Innate immunity

Keywords - Ligandi

Calcium, Metal-binding

Enzyme and pathway databases

BRENDAi3.4.21.45. 3474.
ReactomeiREACT_198562. Regulation of Complement cascade.

Protein family/group databases

MEROPSiS01.199.

Names & Taxonomyi

Protein namesi
Recommended name:
Complement factor I (EC:3.4.21.45)
Alternative name(s):
C3B/C4B inactivator
Cleaved into the following 2 chains:
Gene namesi
Name:Cfi
Synonyms:If
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 3

Organism-specific databases

MGIiMGI:105937. Cfi.

Subcellular locationi

GO - Cellular componenti

  1. extracellular space Source: Ensembl
  2. extracellular vesicular exosome Source: Ensembl
  3. membrane Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1818Sequence AnalysisAdd
BLAST
Chaini19 – 603585Complement factor IPRO_0000027571Add
BLAST
Chaini19 – 356338Complement factor I heavy chainPRO_0000027572Add
BLAST
Chaini361 – 603243Complement factor I light chainPRO_0000027573Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi36 ↔ 260By similarity
Disulfide bondi46 ↔ 57By similarity
Disulfide bondi51 ↔ 62By similarity
Disulfide bondi64 ↔ 96By similarity
Disulfide bondi70 ↔ 89By similarity
Disulfide bondi78 ↔ 109By similarity
Glycosylationi106 – 1061N-linked (GlcNAc...)Sequence Analysis
Glycosylationi116 – 1161N-linked (GlcNAc...)1 Publication
Disulfide bondi144 ↔ 186By similarity
Disulfide bondi157 ↔ 219By similarity
Glycosylationi174 – 1741N-linked (GlcNAc...)Sequence Analysis
Glycosylationi182 – 1821N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi191 ↔ 201By similarity
Disulfide bondi234 ↔ 252By similarity
Disulfide bondi264 ↔ 276By similarity
Glycosylationi267 – 2671N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi271 ↔ 289By similarity
Disulfide bondi283 ↔ 298By similarity
Disulfide bondi348 ↔ 473Interchain (between heavy and light chains)PROSITE-ProRule annotation
Disulfide bondi386 ↔ 402By similarity
Disulfide bondi394 ↔ 464By similarity
Disulfide bondi487 ↔ 551By similarity
Glycosylationi514 – 5141N-linked (GlcNAc...)1 Publication
Disulfide bondi515 ↔ 530By similarity
Disulfide bondi541 ↔ 570By similarity
Glycosylationi556 – 5561N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Cleavage on pair of basic residues, Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiQ61129.
PaxDbiQ61129.
PRIDEiQ61129.

PTM databases

PhosphoSiteiQ61129.

Expressioni

Tissue specificityi

Plasma.

Gene expression databases

BgeeiQ61129.
CleanExiMM_CFI.
GenevestigatoriQ61129.

Interactioni

Subunit structurei

Heterodimer of a light and heavy chains; disulfide-linked. The fully processed and mature protein circulates as a zymogen, and is allosterically activated by substrate-induced remodeling of the active site (By similarity).By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000077074.

Structurei

3D structure databases

ProteinModelPortaliQ61129.
SMRiQ61129. Positions 30-603.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini58 – 11154Kazal-likePROSITE-ProRule annotationAdd
BLAST
Domaini117 – 217101SRCRPROSITE-ProRule annotationAdd
BLAST
Domaini218 – 26245LDL-receptor class A 1PROSITE-ProRule annotationAdd
BLAST
Domaini263 – 29937LDL-receptor class A 2PROSITE-ProRule annotationAdd
BLAST
Domaini361 – 594234Peptidase S1PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase S1 family.PROSITE-ProRule annotation
Contains 1 Kazal-like domain.PROSITE-ProRule annotation
Contains 2 LDL-receptor class A domains.PROSITE-ProRule annotation
Contains 1 peptidase S1 domain.PROSITE-ProRule annotation
Contains 1 SRCR domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiCOG5640.
GeneTreeiENSGT00770000120557.
HOGENOMiHOG000060288.
HOVERGENiHBG005311.
InParanoidiQ61129.
KOiK01333.
OMAiLSCGVKN.
OrthoDBiEOG71G9TN.
TreeFamiTF330647.

Family and domain databases

Gene3Di3.10.250.10. 1 hit.
4.10.400.10. 2 hits.
InterProiIPR003884. FacI_MAC.
IPR002350. Kazal_dom.
IPR023415. LDLR_class-A_CS.
IPR002172. LDrepeatLR_classA_rpt.
IPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR001190. SRCR.
IPR017448. SRCR-like_dom.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamiPF07648. Kazal_2. 1 hit.
PF00057. Ldl_recept_a. 2 hits.
PF00530. SRCR. 1 hit.
PF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00057. FIMAC. 1 hit.
SM00280. KAZAL. 1 hit.
SM00192. LDLa. 2 hits.
SM00202. SR. 1 hit.
SM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
SSF56487. SSF56487. 1 hit.
SSF57424. SSF57424. 2 hits.
PROSITEiPS51465. KAZAL_2. 1 hit.
PS01209. LDLRA_1. 1 hit.
PS50068. LDLRA_2. 2 hits.
PS50287. SRCR_2. 1 hit.
PS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q61129-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKLAHLSLFL LALHLSSSRS PSASDLPQEE LVDQKCLLQK YTHRSCNKVF
60 70 80 90 100
CQPWQRCIEG TCICKLPYQC PRAGTPVCAM NGRSYPTYCH QKSFECLHPE
110 120 130 140 150
IKFSHNGTCA AEGKFNVSLI YGRTKTEGLV QVKLVDQDER MFICKNSWSM
160 170 180 190 200
AEANVACVDL GFPLGVRDIQ GSFNISGNLH INDTECLHVH CRGVETSLAE
210 220 230 240 250
CAFTKRRTEL SNGLAGVVCY KQDADFPTSL SFQCVNGKHI PQEKACNGVN
260 270 280 290 300
DCGDQSDELC CKGCRGNASL CKSGVCIPDQ YKCNGEVDCI TGEDESRCEE
310 320 330 340 350
DRQQNIPKGL ARSAQGEAEI ETEETEMLTP GMDNERKRIK SLLPKLSCGV
360 370 380 390 400
KRNTHTRRKR VIGGKPANVG DYPWQVAIKD GQRITCGGIY IGGCWILTAA
410 420 430 440 450
HCVRPSRAHS YQVWTALLDW LKPNSQLGIQ TVKRVIVHEK YNGATFQNDI
460 470 480 490 500
ALIEMKMHTG KKECELPNSV PACVPWSPYL FQPNDRCIIS GWGRGKDNQK
510 520 530 540 550
VYSLRWGEVD LIGNCSQFYP DRYYEKEMQC AGTRDGSIDA CKGDSGGPLV
560 570 580 590 600
CEDINNVTYV WGIVSWGENC GKPEFPGVYT RVANYFDWIS YHVGRSLVSQ

HNV
Length:603
Mass (Da):67,261
Last modified:July 27, 2011 - v3
Checksum:iA9B90E1EC78AEE37
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti114 – 1141K → N in AAB00438. (PubMed:8604219)Curated
Sequence conflicti236 – 25217NGKHI…GVNDC → MGSTFLRRKPATVSMTV in AAB00438. (PubMed:8604219)CuratedAdd
BLAST
Sequence conflicti554 – 5541I → T in AAB00438. (PubMed:8604219)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U47810 mRNA. Translation: AAB00438.1.
BC150751 mRNA. Translation: AAI50752.1.
AH007741 Genomic DNA. Translation: AAD32965.1.
CCDSiCCDS17835.1.
RefSeqiNP_031712.2. NM_007686.2.
XP_006501023.1. XM_006500960.1.
UniGeneiMm.117180.

Genome annotation databases

EnsembliENSMUST00000077918; ENSMUSP00000077074; ENSMUSG00000058952.
GeneIDi12630.
KEGGimmu:12630.
UCSCiuc008rin.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U47810 mRNA. Translation: AAB00438.1 .
BC150751 mRNA. Translation: AAI50752.1 .
AH007741 Genomic DNA. Translation: AAD32965.1 .
CCDSi CCDS17835.1.
RefSeqi NP_031712.2. NM_007686.2.
XP_006501023.1. XM_006500960.1.
UniGenei Mm.117180.

3D structure databases

ProteinModelPortali Q61129.
SMRi Q61129. Positions 30-603.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 10090.ENSMUSP00000077074.

Protein family/group databases

MEROPSi S01.199.

PTM databases

PhosphoSitei Q61129.

Proteomic databases

MaxQBi Q61129.
PaxDbi Q61129.
PRIDEi Q61129.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000077918 ; ENSMUSP00000077074 ; ENSMUSG00000058952 .
GeneIDi 12630.
KEGGi mmu:12630.
UCSCi uc008rin.1. mouse.

Organism-specific databases

CTDi 3426.
MGIi MGI:105937. Cfi.

Phylogenomic databases

eggNOGi COG5640.
GeneTreei ENSGT00770000120557.
HOGENOMi HOG000060288.
HOVERGENi HBG005311.
InParanoidi Q61129.
KOi K01333.
OMAi LSCGVKN.
OrthoDBi EOG71G9TN.
TreeFami TF330647.

Enzyme and pathway databases

BRENDAi 3.4.21.45. 3474.
Reactomei REACT_198562. Regulation of Complement cascade.

Miscellaneous databases

NextBioi 281812.
PROi Q61129.
SOURCEi Search...

Gene expression databases

Bgeei Q61129.
CleanExi MM_CFI.
Genevestigatori Q61129.

Family and domain databases

Gene3Di 3.10.250.10. 1 hit.
4.10.400.10. 2 hits.
InterProi IPR003884. FacI_MAC.
IPR002350. Kazal_dom.
IPR023415. LDLR_class-A_CS.
IPR002172. LDrepeatLR_classA_rpt.
IPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR001190. SRCR.
IPR017448. SRCR-like_dom.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view ]
Pfami PF07648. Kazal_2. 1 hit.
PF00057. Ldl_recept_a. 2 hits.
PF00530. SRCR. 1 hit.
PF00089. Trypsin. 1 hit.
[Graphical view ]
PRINTSi PR00722. CHYMOTRYPSIN.
SMARTi SM00057. FIMAC. 1 hit.
SM00280. KAZAL. 1 hit.
SM00192. LDLa. 2 hits.
SM00202. SR. 1 hit.
SM00020. Tryp_SPc. 1 hit.
[Graphical view ]
SUPFAMi SSF50494. SSF50494. 1 hit.
SSF56487. SSF56487. 1 hit.
SSF57424. SSF57424. 2 hits.
PROSITEi PS51465. KAZAL_2. 1 hit.
PS01209. LDLRA_1. 1 hit.
PS50068. LDLRA_2. 2 hits.
PS50287. SRCR_2. 1 hit.
PS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "cDNA cloning, sequencing and chromosomal assignment of the gene for mouse complement factor I (C3b/C4b inactivator): identification of a species specific divergent segment in factor I."
    Minta J.O., Wong M.J., Kozak C.A., Kunnath-Muglia L.M., Goldberger G.
    Mol. Immunol. 33:101-112(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: BALB/c.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain.
  3. "Cloning and characterization of the non-catalytic heavy chain of mouse complement factor I gene: structure comparison with the human homologue."
    Yun Y.-S., Goldberger G., Minta J.O.
    Biochem. Mol. Biol. Int. 47:493-500(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 114-334.
    Strain: 129/Sv.
    Tissue: Kidney.
  4. "Proteome-wide characterization of N-glycosylation events by diagonal chromatography."
    Ghesquiere B., Van Damme J., Martens L., Vandekerckhove J., Gevaert K.
    J. Proteome Res. 5:2438-2447(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-116.
    Strain: C57BL/6.
    Tissue: Plasma.
  5. "Enhanced analysis of the mouse plasma proteome using cysteine-containing tryptic glycopeptides."
    Bernhard O.K., Kapp E.A., Simpson R.J.
    J. Proteome Res. 6:987-995(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-514.
    Strain: C57BL/6.
    Tissue: Plasma.

Entry informationi

Entry nameiCFAI_MOUSE
AccessioniPrimary (citable) accession number: Q61129
Secondary accession number(s): B2RWX8, Q9WU07
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 4, 2001
Last sequence update: July 27, 2011
Last modified: November 26, 2014
This is version 140 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3