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Q61127 (NAB2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 91. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
NGFI-A-binding protein 2
Alternative name(s):
EGR-1-binding protein 2
Gene names
Name:Nab2
OrganismMus musculus (Mouse)
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length525 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Acts as a transcriptional repressor for zinc finger transcription factors EGR1 and EGR2. Isoform 2 lacks repression ability.

Subunit structure

Homomultimers may associate with EGR1 bound to DNA By similarity.

Subcellular location

Nucleus. Note: Isoform 2 is not localized to the nucleus.

Tissue specificity

Highly expressed in brain and thymus, and at lower levels in spleen, kidney, heart and testis. Isoform 1 is predominantely expressed in testis, wheras isoform 3 is more abundant in thymus.

Induction

By serum stimulation.

Domain

The NAB conserved domain 1 (NCD1) interacts with EGR1 inhibitory domain and mediates multimerization.

The NAB conserved domain 2 (NCD2) is necessary for transcriptional repression.

Post-translational modification

Sumoylation by EGR2 represses EGR2 transcriptional activity in hindbrain By similarity.

Sequence similarities

Belongs to the NAB family.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q61127-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q61127-2)

The sequence of this isoform differs from the canonical sequence as follows:
     320-322: LTI → ASL
     323-525: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 525525NGFI-A-binding protein 2
PRO_0000077043

Regions

Region35 – 11379NCD1
Region267 – 35690NCD2
Region353 – 38432Necessary for nuclear localization By similarity

Amino acid modifications

Modified residue61Phosphoserine By similarity
Modified residue1591Phosphoserine Ref.5
Modified residue1621Phosphoserine Ref.5
Modified residue1711Phosphoserine Ref.5 Ref.6
Modified residue4791Phosphoserine By similarity
Cross-link379Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1) By similarity
Cross-link517Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1) By similarity

Natural variations

Alternative sequence320 – 3223LTI → ASL in isoform 2.
VSP_003388
Alternative sequence323 – 525203Missing in isoform 2.
VSP_003389

Experimental info

Sequence conflict2331G → C in AAC52650. Ref.1
Sequence conflict4651R → W in AAC52650. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified July 27, 2011. Version 2.
Checksum: 4FFA510C8E39DF70

FASTA52556,577
        10         20         30         40         50         60 
MHRAPSPTAE QPPGRGDNTR RTPQPRFKAS APAMALPRTL GELQLYRVLQ RANLLSYYET 

        70         80         90        100        110        120 
FIQQGGDDVQ QLCEAGEEEF LEIMALVGMA TKPLHVRRLQ KALREWATNP GLFSQPVPAV 

       130        140        150        160        170        180 
PVSSIPLFKI SETAGTRKGS MSNGHGSPGE KAGSARSFSP KSPLELGEKL SPLPGGPGAG 

       190        200        210        220        230        240 
DPRIWPGQST PESDVGAGGE EEAGSPPFSP PAGGGVSEGP GVGGVAAGGA GGGPDRLEPE 

       250        260        270        280        290        300 
MVRMVVESVE RIFRSFPRGD TGEIASLLKL NKKLARSVGH IFEMDDHDAQ KEEEIRKYSV 

       310        320        330        340        350        360 
IYGRLDSKRR EGKQLSLHEL TINEAAAQFC MRDNTLLLRR VELFSLSRQV ARESTYLSSL 

       370        380        390        400        410        420 
KGSRLHSEEL GGPPLKKLKQ EVGEQSHNEI QQPPPGPESY APPYRPSLEE DSASLSGESL 

       430        440        450        460        470        480 
DGHLQAVGSC PRLTPPPADL PLALPAHGLW SRHILQQTLM DEGLRLARLV SHDRVGRLSP 

       490        500        510        520 
CVPAKPPLAE FEEGLLDRCP APGPHPALVE GRRSSVKVEA EASRQ

« Hide

Isoform 2 [UniParc].

Checksum: 61C0C1BAF842719D
Show »

FASTA32234,376

References

« Hide 'large scale' references
[1]"NAB2, a corepressor of NGFI-A (Egr-1) and Krox20, is induced by proliferative and differentiative stimuli."
Svaren J., Sevetson B.R., Apel E.D., Zimonjic D.B., Popescu N.C., Milbrandt J.
Mol. Cell. Biol. 16:3545-3553(1996) [PubMed: 8668170] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2).
Tissue: Brain.
[2]"The Nab2 and Stat6 genes share a common transcription termination region."
Svaren J., Apel E.D., Simburger K.S., Jenkins N.A., Gilbert D.J., Copeland N.G., Milbrandt J.
Genomics 41:33-39(1997) [PubMed: 9126479] [Abstract]
Cited for: PARTIAL NUCLEOTIDE SEQUENCE (ISOFORM 2).
[3]Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Olfactory epithelium.
[5]"A differential phosphoproteomic analysis of retinoic acid-treated P19 cells."
Smith J.C., Duchesne M.A., Tozzi P., Ethier M., Figeys D.
J. Proteome Res. 6:3174-3186(2007) [PubMed: 17622165] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-159; SER-162 AND SER-171, MASS SPECTROMETRY.
Tissue: Teratocarcinoma.
[6]"Large scale localization of protein phosphorylation by use of electron capture dissociation mass spectrometry."
Sweet S.M., Bailey C.M., Cunningham D.L., Heath J.K., Cooper H.J.
Mol. Cell. Proteomics 8:904-912(2009) [PubMed: 19131326] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-171, MASS SPECTROMETRY.
Tissue: Embryonic fibroblast.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		U47543 mRNA. Translation: AAC52650.1.
CH466578 Genomic DNA. Translation: EDL24515.1.
BC045139 mRNA. Translation: AAH45139.1.
IPIIPI00117780.
IPI00230620.
RefSeqNP_001116367.1. NM_001122895.1.
NP_032694.2. NM_008668.2.
UniGeneMm.336898.
Mm.470656.

3D structure databases

ProteinModelPortalQ61127.
SMRQ61127. Positions 232-366.
ModBaseSearch...

Protein-protein interaction databases

IntActQ61127. 1 interaction.
MINTMINT-2737132.
STRINGQ61127.

PTM databases

PhosphoSiteQ61127.

Proteomic databases

PRIDEQ61127.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000026469; ENSMUSP00000026469; ENSMUSG00000025402.
GeneID17937.
KEGGmmu:17937.

Organism-specific databases

CTD4665.
MGIMGI:107563. Nab2.

Phylogenomic databases

GeneTreeENSGT00390000006330.
HOGENOMHBG447154.
HOVERGENHBG003127.
InParanoidQ61127.

Gene expression databases

ArrayExpressQ61127.
BgeeQ61127.
CleanExMM_NAB2.
GenevestigatorQ61127.
GermOnlineENSMUSG00000025402. Mus musculus.

Family and domain databases

InterProIPR006989. NAB_co-repressor_dom.
IPR006988. Nab_N.
[Graphical view]
PfamPF04904. NCD1. 1 hit.
PF04905. NCD2. 1 hit.
[Graphical view]
ProDomPD342192. Nab_N. 1 hit.
[Graphical view] [Entries sharing at least one domain]
ProtoNetSearch...

Other

NextBio292837.
SOURCESearch...

Entry information

Entry nameNAB2_MOUSE
AccessionPrimary (citable) accession number: Q61127
Secondary accession number(s): Q80VR9
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: July 27, 2011
Last modified: December 14, 2011
This is version 91 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

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