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Protein

NGFI-A-binding protein 2

Gene

Nab2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Acts as a transcriptional repressor for zinc finger transcription factors EGR1 and EGR2. Isoform 2 lacks repression ability.

GO - Molecular functioni

  • transcription factor binding Source: MGI

GO - Biological processi

  • endochondral ossification Source: MGI
  • myelination Source: MGI
  • negative regulation of transcription from RNA polymerase III promoter Source: MGI
  • regulation of epidermis development Source: MGI
  • regulation of transcription, DNA-templated Source: MGI
  • Schwann cell differentiation Source: MGI
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
NGFI-A-binding protein 2
Alternative name(s):
EGR-1-binding protein 2
Gene namesi
Name:Nab2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 10

Organism-specific databases

MGIiMGI:107563. Nab2.

Subcellular locationi

  • Nucleus

  • Note: Isoform 2 is not localized to the nucleus.

GO - Cellular componenti

  • nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 525525NGFI-A-binding protein 2PRO_0000077043Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei6 – 61PhosphoserineBy similarity
Modified residuei157 – 1571PhosphoserineCombined sources
Modified residuei159 – 1591PhosphoserineCombined sources
Modified residuei162 – 1621PhosphoserineCombined sources
Modified residuei171 – 1711PhosphoserineCombined sources
Cross-linki379 – 379Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1)By similarity
Modified residuei479 – 4791PhosphoserineCombined sources
Cross-linki517 – 517Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1)By similarity

Post-translational modificationi

Sumoylation by EGR2 represses EGR2 transcriptional activity in hindbrain.By similarity

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ61127.
MaxQBiQ61127.
PaxDbiQ61127.
PRIDEiQ61127.

PTM databases

iPTMnetiQ61127.
PhosphoSiteiQ61127.

Expressioni

Tissue specificityi

Highly expressed in brain and thymus, and at lower levels in spleen, kidney, heart and testis. Isoform 1 is predominantly expressed in testis, whereas isoform 3 is more abundant in thymus.

Inductioni

By serum stimulation.

Gene expression databases

BgeeiQ61127.
CleanExiMM_NAB2.
ExpressionAtlasiQ61127. baseline and differential.
GenevisibleiQ61127. MM.

Interactioni

Subunit structurei

Homomultimers may associate with EGR1 bound to DNA.By similarity

GO - Molecular functioni

  • transcription factor binding Source: MGI

Protein-protein interaction databases

BioGridi201681. 3 interactions.
IntActiQ61127. 4 interactions.
MINTiMINT-2737132.
STRINGi10090.ENSMUSP00000026469.

Structurei

3D structure databases

ProteinModelPortaliQ61127.
SMRiQ61127. Positions 228-356.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni35 – 11379NCD1Add
BLAST
Regioni267 – 35690NCD2Add
BLAST
Regioni353 – 38432Necessary for nuclear localizationBy similarityAdd
BLAST

Domaini

The NAB conserved domain 1 (NCD1) interacts with EGR1 inhibitory domain and mediates multimerization.
The NAB conserved domain 2 (NCD2) is necessary for transcriptional repression.

Sequence similaritiesi

Belongs to the NAB family.Curated

Phylogenomic databases

eggNOGiKOG3835. Eukaryota.
ENOG410YKFH. LUCA.
GeneTreeiENSGT00390000006330.
HOGENOMiHOG000026777.
HOVERGENiHBG003127.
InParanoidiQ61127.
OMAiHSELQQP.
OrthoDBiEOG776SPQ.
TreeFamiTF315501.

Family and domain databases

InterProiIPR006989. NAB_co-repressor_dom.
IPR006988. Nab_N.
[Graphical view]
PfamiPF04904. NCD1. 1 hit.
PF04905. NCD2. 1 hit.
[Graphical view]
ProDomiPD342192. Nab_N. 1 hit.
[Graphical view] [Entries sharing at least one domain]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q61127-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MHRAPSPTAE QPPGRGDNTR RTPQPRFKAS APAMALPRTL GELQLYRVLQ
60 70 80 90 100
RANLLSYYET FIQQGGDDVQ QLCEAGEEEF LEIMALVGMA TKPLHVRRLQ
110 120 130 140 150
KALREWATNP GLFSQPVPAV PVSSIPLFKI SETAGTRKGS MSNGHGSPGE
160 170 180 190 200
KAGSARSFSP KSPLELGEKL SPLPGGPGAG DPRIWPGQST PESDVGAGGE
210 220 230 240 250
EEAGSPPFSP PAGGGVSEGP GVGGVAAGGA GGGPDRLEPE MVRMVVESVE
260 270 280 290 300
RIFRSFPRGD TGEIASLLKL NKKLARSVGH IFEMDDHDAQ KEEEIRKYSV
310 320 330 340 350
IYGRLDSKRR EGKQLSLHEL TINEAAAQFC MRDNTLLLRR VELFSLSRQV
360 370 380 390 400
ARESTYLSSL KGSRLHSEEL GGPPLKKLKQ EVGEQSHNEI QQPPPGPESY
410 420 430 440 450
APPYRPSLEE DSASLSGESL DGHLQAVGSC PRLTPPPADL PLALPAHGLW
460 470 480 490 500
SRHILQQTLM DEGLRLARLV SHDRVGRLSP CVPAKPPLAE FEEGLLDRCP
510 520
APGPHPALVE GRRSSVKVEA EASRQ
Length:525
Mass (Da):56,577
Last modified:July 27, 2011 - v2
Checksum:i4FFA510C8E39DF70
GO
Isoform 2 (identifier: Q61127-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     320-322: LTI → ASL
     323-525: Missing.

Show »
Length:322
Mass (Da):34,376
Checksum:i61C0C1BAF842719D
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti233 – 2331G → C in AAC52650 (PubMed:8668170).Curated
Sequence conflicti465 – 4651R → W in AAC52650 (PubMed:8668170).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei320 – 3223LTI → ASL in isoform 2. 1 PublicationVSP_003388
Alternative sequencei323 – 525203Missing in isoform 2. 1 PublicationVSP_003389Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U47543 mRNA. Translation: AAC52650.1.
CH466578 Genomic DNA. Translation: EDL24515.1.
BC045139 mRNA. Translation: AAH45139.1.
CCDSiCCDS24247.1. [Q61127-1]
RefSeqiNP_001116367.1. NM_001122895.1.
NP_032694.2. NM_008668.2. [Q61127-1]
UniGeneiMm.336898.
Mm.470656.

Genome annotation databases

EnsembliENSMUST00000026469; ENSMUSP00000026469; ENSMUSG00000025402. [Q61127-1]
GeneIDi17937.
KEGGimmu:17937.
UCSCiuc011xpt.1. mouse. [Q61127-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U47543 mRNA. Translation: AAC52650.1.
CH466578 Genomic DNA. Translation: EDL24515.1.
BC045139 mRNA. Translation: AAH45139.1.
CCDSiCCDS24247.1. [Q61127-1]
RefSeqiNP_001116367.1. NM_001122895.1.
NP_032694.2. NM_008668.2. [Q61127-1]
UniGeneiMm.336898.
Mm.470656.

3D structure databases

ProteinModelPortaliQ61127.
SMRiQ61127. Positions 228-356.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi201681. 3 interactions.
IntActiQ61127. 4 interactions.
MINTiMINT-2737132.
STRINGi10090.ENSMUSP00000026469.

PTM databases

iPTMnetiQ61127.
PhosphoSiteiQ61127.

Proteomic databases

EPDiQ61127.
MaxQBiQ61127.
PaxDbiQ61127.
PRIDEiQ61127.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000026469; ENSMUSP00000026469; ENSMUSG00000025402. [Q61127-1]
GeneIDi17937.
KEGGimmu:17937.
UCSCiuc011xpt.1. mouse. [Q61127-1]

Organism-specific databases

CTDi4665.
MGIiMGI:107563. Nab2.

Phylogenomic databases

eggNOGiKOG3835. Eukaryota.
ENOG410YKFH. LUCA.
GeneTreeiENSGT00390000006330.
HOGENOMiHOG000026777.
HOVERGENiHBG003127.
InParanoidiQ61127.
OMAiHSELQQP.
OrthoDBiEOG776SPQ.
TreeFamiTF315501.

Miscellaneous databases

ChiTaRSiNab2. mouse.
PROiQ61127.
SOURCEiSearch...

Gene expression databases

BgeeiQ61127.
CleanExiMM_NAB2.
ExpressionAtlasiQ61127. baseline and differential.
GenevisibleiQ61127. MM.

Family and domain databases

InterProiIPR006989. NAB_co-repressor_dom.
IPR006988. Nab_N.
[Graphical view]
PfamiPF04904. NCD1. 1 hit.
PF04905. NCD2. 1 hit.
[Graphical view]
ProDomiPD342192. Nab_N. 1 hit.
[Graphical view] [Entries sharing at least one domain]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "NAB2, a corepressor of NGFI-A (Egr-1) and Krox20, is induced by proliferative and differentiative stimuli."
    Svaren J., Sevetson B.R., Apel E.D., Zimonjic D.B., Popescu N.C., Milbrandt J.
    Mol. Cell. Biol. 16:3545-3553(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2).
    Tissue: Brain.
  2. "The Nab2 and Stat6 genes share a common transcription termination region."
    Svaren J., Apel E.D., Simburger K.S., Jenkins N.A., Gilbert D.J., Copeland N.G., Milbrandt J.
    Genomics 41:33-39(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: PARTIAL NUCLEOTIDE SEQUENCE (ISOFORM 2).
  3. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Olfactory epithelium.
  5. "Large scale localization of protein phosphorylation by use of electron capture dissociation mass spectrometry."
    Sweet S.M., Bailey C.M., Cunningham D.L., Heath J.K., Cooper H.J.
    Mol. Cell. Proteomics 8:904-912(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-171, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Embryonic fibroblast.
  6. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-157; SER-159; SER-162; SER-171 AND SER-479, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Brown adipose tissue, Heart, Kidney, Lung, Spleen and Testis.

Entry informationi

Entry nameiNAB2_MOUSE
AccessioniPrimary (citable) accession number: Q61127
Secondary accession number(s): Q80VR9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: July 27, 2011
Last modified: June 8, 2016
This is version 122 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.