Reviewed, UniProtKB/Swiss-Prot Q61127 (NAB2_MOUSE)

Names and origin

Protein names

Recommended name:
    NGFI-A-binding protein 2
Alternative name(s):
    EGR-1-binding protein 2

Gene names

Name:

Nab2

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	525 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Acts as a transcriptional repressor for zinc finger transcription factors EGR1 and EGR2. Isoform 2 lacks repression ability.
Subunit structure	Homomultimers may associate with EGR1 bound to DNA By similarity.
Subcellular location	Nucleus. Note: Isoform 2 is not localized to the nucleus.
Tissue specificity	Highly expressed in brain and thymus, and at lower levels in spleen, kidney, heart and testis. Isoform 1 is predominantely expressed in testis, wheras isoform 3 is more abundant in thymus.
Induction	By serum stimulation.
Domain	The NAB conserved domain 1 (NCD1) interacts with EGR1 inhibitory domain and mediates multimerization. The NAB conserved domain 2 (NCD2) is necessary for transcriptional repression.
Sequence similarities	Belongs to the NAB family.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing
Molecular function	Repressor
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	Schwann cell differentiation Inferred from genetic interaction. Source: MGI endochondral ossification Inferred from genetic interaction. Source: MGI myelination Inferred from genetic interaction. Source: MGI negative regulation of transcription Inferred from electronic annotation. Source: InterPro regulation of epidermis development Inferred from genetic interaction. Source: MGI regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transcription Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	transcription repressor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q61127-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q61127-2)

The sequence of this isoform differs from the canonical sequence as follows:
     320-322: LTI → ASL
     323-525: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 525	525	NGFI-A-binding protein 2		PRO_0000077043
Regions
	Region	35 – 113	79	NCD1
	Region	267 – 356	90	NCD2
	Region	353 – 384	32	Necessary for nuclear localization By similarity
Amino acid modifications
	Modified residue	6	1	Phosphoserine By similarity
	Modified residue	159	1	Phosphoserine Ref.3
	Modified residue	162	1	Phosphoserine Ref.3
	Modified residue	171	1	Phosphoserine Ref.3
	Modified residue	479	1	Phosphoserine By similarity
Natural variations
	Alternative sequence	320 – 322	3	LTI → ASL in isoform 2.		VSP_003388
	Alternative sequence	323 – 525	203	Missing in isoform 2.		VSP_003389

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: FED428E94A8BD804	FASTA	525	56,653
	10 20 30 40 50 60 MHRAPSPTAE QPPGRGDNTR RTPQPRFKAS APAMALPRTL GELQLYRVLQ RANLLSYYET 70 80 90 100 110 120 FIQQGGDDVQ QLCEAGEEEF LEIMALVGMA TKPLHVRRLQ KALREWATNP GLFSQPVPAV 130 140 150 160 170 180 PVSSIPLFKI SETAGTRKGS MSNGHGSPGE KAGSARSFSP KSPLELGEKL SPLPGGPGAG 190 200 210 220 230 240 DPRIWPGQST PESDVGAGGE EEAGSPPFSP PAGGGVSEGP GVGGVAAGGA GGCPDRLEPE 250 260 270 280 290 300 MVRMVVESVE RIFRSFPRGD TGEIASLLKL NKKLARSVGH IFEMDDHDAQ KEEEIRKYSV 310 320 330 340 350 360 IYGRLDSKRR EGKQLSLHEL TINEAAAQFC MRDNTLLLRR VELFSLSRQV ARESTYLSSL 370 380 390 400 410 420 KGSRLHSEEL GGPPLKKLKQ EVGEQSHNEI QQPPPGPESY APPYRPSLEE DSASLSGESL 430 440 450 460 470 480 DGHLQAVGSC PRLTPPPADL PLALPAHGLW SRHILQQTLM DEGLWLARLV SHDRVGRLSP 490 500 510 520 CVPAKPPLAE FEEGLLDRCP APGPHPALVE GRRSSVKVEA EASRQ
	Isoform 2. Checksum: 61C485BEB80675D9	FASTA	322	34,422
	10 20 30 40 50 60 MHRAPSPTAE QPPGRGDNTR RTPQPRFKAS APAMALPRTL GELQLYRVLQ RANLLSYYET 70 80 90 100 110 120 FIQQGGDDVQ QLCEAGEEEF LEIMALVGMA TKPLHVRRLQ KALREWATNP GLFSQPVPAV 130 140 150 160 170 180 PVSSIPLFKI SETAGTRKGS MSNGHGSPGE KAGSARSFSP KSPLELGEKL SPLPGGPGAG 190 200 210 220 230 240 DPRIWPGQST PESDVGAGGE EEAGSPPFSP PAGGGVSEGP GVGGVAAGGA GGCPDRLEPE 250 260 270 280 290 300 MVRMVVESVE RIFRSFPRGD TGEIASLLKL NKKLARSVGH IFEMDDHDAQ KEEEIRKYSV 310 320 IYGRLDSKRR EGKQLSLHEA SL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"NAB2, a corepressor of NGFI-A (Egr-1) and Krox20, is induced by proliferative and differentiative stimuli." Svaren J., Sevetson B.R., Apel E.D., Zimonjic D.B., Popescu N.C., Milbrandt J. Mol. Cell. Biol. 16:3545-3553(1996) [PubMed: 8668170] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2). Tissue: Brain.
[2]	"The Nab2 and Stat6 genes share a common transcription termination region." Svaren J., Apel E.D., Simburger K.S., Jenkins N.A., Gilbert D.J., Copeland N.G., Milbrandt J. Genomics 41:33-39(1997) [PubMed: 9126479] [Abstract] Cited for: PARTIAL NUCLEOTIDE SEQUENCE (ISOFORM 2).
[3]	"A differential phosphoproteomic analysis of retinoic acid-treated P19 cells." Smith J.C., Duchesne M.A., Tozzi P., Ethier M., Figeys D. J. Proteome Res. 6:3174-3186(2007) [PubMed: 17622165] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-159; SER-162 AND SER-171, MASS SPECTROMETRY.
[4]	"Large scale localization of protein phosphorylation by use of electron capture dissociation mass spectrometry." Sweet S.M., Bailey C.M., Cunningham D.L., Heath J.K., Cooper H.J. Mol. Cell. Proteomics 8:904-912(2009) [PubMed: 19131326] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-171, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U47543 mRNA. Translation: AAC52650.1.
IPI	IPI00117780. IPI00875366.
UniGene	Mm.336898 Mm.470656
3D structure databases
ModBase	Search...
PTM databases
PhosphoSite	Q61127.
Proteomic databases
PRIDE	Q61127.
Genome annotation databases
Ensembl	ENSMUSG00000025402. Mus musculus. [Contig view]
Organism-specific databases
MGI	MGI:107563. Nab2.
Phylogenomic databases
HOGENOM	Q61127.
HOVERGEN	Q61127.
Gene expression databases
ArrayExpress	Q61127.
Bgee	Q61127.
CleanEx	MM_NAB2.
GermOnline	ENSMUSG00000025402. Mus musculus.
Family and domain databases
InterPro	IPR006989. Nab_centr. IPR006988. Nab_N. [Graphical view]
Pfam	PF04904. NCD1. 1 hit. PF04905. NCD2. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
SOURCE	Search...

Entry information

Entry name

NAB2_MOUSE

Accession

Primary (citable) accession number: Q61127

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	November 1, 1996
Last modified:	June 16, 2009
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)