Q61125 (BKRB1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
B1 bradykinin receptor
Short name=B1R
Short name=BK-1 receptor

Gene names

Name:	Bdkrb1
Synonyms:	Bdkrb

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	334 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	This is a receptor for bradykinin. Could be a factor in chronic pain and inflammation.
Subcellular location	Cell membrane; Multi-pass membrane protein.
Tissue specificity	Expressed in heart, liver and lung.
Induction	By lipopolysaccharide (LPS) in heart, liver and lung five hours after treatment. Ref.1
Sequence similarities	Belongs to the G-protein coupled receptor 1 family. Bradykinin receptor subfamily. BDKRB1 sub-subfamily.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	G-protein coupled receptor Receptor Transducer
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell migration Inferred from electronic annotation. Source: Compara elevation of cytosolic calcium ion concentration Inferred from electronic annotation. Source: InterPro inflammatory response Inferred from electronic annotation. Source: Compara negative regulation of blood pressure Inferred from electronic annotation. Source: Compara negative regulation of cell growth Inferred from electronic annotation. Source: Compara negative regulation of protein phosphorylation Inferred from electronic annotation. Source: Compara positive regulation of leukocyte migration Inferred from electronic annotation. Source: Compara positive regulation of release of sequestered calcium ion into cytosol Inferred from electronic annotation. Source: Compara protein kinase C-activating G-protein coupled receptor signaling pathway Inferred from electronic annotation. Source: Compara response to lipopolysaccharide Inferred from direct assay Ref.1. Source: MGI sensory perception of pain Inferred from electronic annotation. Source: Compara
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW neuron projection Inferred from electronic annotation. Source: Compara plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	bradykinin receptor activity Inferred from electronic annotation. Source: Compara peptide binding Inferred from direct assay Ref.1. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 334	334	B1 bradykinin receptor		PRO_0000069184
Regions
	Topological domain	1 – 41	41	Extracellular Potential
	Transmembrane	42 – 62	21	Helical; Name=1; Potential
	Topological domain	63 – 80	18	Cytoplasmic Potential
	Transmembrane	81 – 101	21	Helical; Name=2; Potential
	Topological domain	102 – 118	17	Extracellular Potential
	Transmembrane	119 – 139	21	Helical; Name=3; Potential
	Topological domain	140 – 161	22	Cytoplasmic Potential
	Transmembrane	162 – 182	21	Helical; Name=4; Potential
	Topological domain	183 – 214	32	Extracellular Potential
	Transmembrane	215 – 235	21	Helical; Name=5; Potential
	Topological domain	236 – 258	23	Cytoplasmic Potential
	Transmembrane	259 – 279	21	Helical; Name=6; Potential
	Topological domain	280 – 302	23	Extracellular Potential
	Transmembrane	303 – 323	21	Helical; Name=7; Potential
	Topological domain	324 – 334	11	Cytoplasmic Potential
Amino acid modifications
	Glycosylation	13	1	N-linked (GlcNAc...) Potential
	Glycosylation	21	1	N-linked (GlcNAc...) Potential
	Glycosylation	192	1	N-linked (GlcNAc...) Potential
	Disulfide bond	117 ↔ 196		By similarity
Experimental info
	Sequence conflict	216	1	L → V in AAD00029. Ref.2
	Sequence conflict	260	1	L → P Ref.2
	Sequence conflict	263 – 265	3	VAS → SSL Ref.2
	Sequence conflict	331 – 334	4	LGTL → WELYKRCTSLKNAVLPSRKE LFQLSCWN in AAD00029. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q61125 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 77C806203AC5C6CC	FASTA	334	37,886
	10 20 30 40 50 60 MASQASLKLQ PSNQSQQAPP NITSCEGAPE AWDLLCRVLP GFVITVCFFG LLGNLLVLSF 70 80 90 100 110 120 FLLPWRRWWQ QRRQRLTIAE IYLANLAASD LVFVLGLPFW AENVGNRFNW PFGSDLCRVV 130 140 150 160 170 180 SGVIKANLFI SIFLVVAISQ DRYRLLVYPM TSWGNRRRRQ AQVTCLLIWV AGGLLSTPTF 190 200 210 220 230 240 LLRSVKVVPD LNISACILLF PHEAWHFVRM VELNVLGFLL PLAAILYFNF HILASLRGQK 250 260 270 280 290 300 EASRTRCGGP KDSKTMGLIL TLVASFLVCW APYHFFAFLD FLVQVRVIQD CFWKELTDLG 310 320 330 LQLANFFAFV NSCLNPLIYV FAGRLFKTRV LGTL

References

[1]	"Molecular cloning and functional characterization of a mouse bradykinin B1 receptor gene." Pesquero J.B., Pesquero J.L., Oliveira S.M., Roscher A.A., Metzger R., Ganten D., Bader M. Biochem. Biophys. Res. Commun. 220:219-225(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], INDUCTION. Strain: 129/SvJ.
[2]	"Novel pharmacological selectivity of the cloned murine B1 bradykinin receptor." Yanagawa T., Prado G.N., Paquet J.L., Bestian S., Cousins B., Navarro J. Submitted (JAN-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U47281 Genomic DNA. Translation: AAA99778.1. U44436 Genomic DNA. Translation: AAD00029.1.
IPI	IPI00117779.
PIR	JC4681.
RefSeq	NP_031565.1. NM_007539.2.
UniGene	Mm.377078.
3D structure databases
ProteinModelPortal	Q61125.
SMR	Q61125. Positions 46-334.
ModBase	Search...
Protein family/group databases
GPCRDB	Search...
Proteomic databases
PRIDE	Q61125.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000041229; ENSMUSP00000045335; ENSMUSG00000041347.
GeneID	12061.
KEGG	mmu:12061.
UCSC	uc007oyn.1. mouse.
Organism-specific databases
CTD	623.
MGI	MGI:88144. Bdkrb1.
Phylogenomic databases
eggNOG	NOG327312.
GeneTree	ENSGT00700000104167.
HOGENOM	HOG000010314.
HOVERGEN	HBG103183.
InParanoid	Q61125.
KO	K03915.
OMA	PYHFFAF.
OrthoDB	EOG4WH8M4.
Gene expression databases
ArrayExpress	Q61125.
Bgee	Q61125.
CleanEx	MM_BDKRB1.
Genevestigator	Q61125.
GermOnline	ENSMUSG00000041347. Mus musculus.
Family and domain databases
InterPro	IPR001186. Brdyknn_1_rcpt. IPR000496. Brdyknn_rcpt. IPR000276. GPCR_Rhodpsn. IPR017452. GPCR_Rhodpsn_7TM. [Graphical view]
Pfam	PF00001. 7tm_1. 1 hit. [Graphical view]
PRINTS	PR00425. BRADYKININR. PR00993. BRADYKINNB1R. PR00237. GPCRRHODOPSN.
PROSITE	PS00237. G_PROTEIN_RECEP_F1_1. False negative. PS50262. G_PROTEIN_RECEP_F1_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
BindingDB	Q61125.
ChEMBL	CHEMBL1250407.
NextBio	280369.
SOURCE	Search...

Entry information

Entry name

BKRB1_MOUSE

Accession

Primary (citable) accession number: Q61125
Secondary accession number(s): Q9Z1F4

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 27, 2001
Last sequence update:	November 1, 1996
Last modified:	May 29, 2013
This is version 108 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program