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Protein

NGFI-A-binding protein 1

Gene

Nab1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Acts as a transcriptional repressor for zinc finger transcription factors EGR1 and EGR2.By similarity1 Publication

GO - Molecular functioni

  • transcription factor binding Source: MGI

GO - Biological processi

  • endochondral ossification Source: MGI
  • myelination Source: MGI
  • negative regulation of transcription, DNA-templated Source: InterPro
  • regulation of epidermis development Source: MGI
  • regulation of transcription, DNA-templated Source: MGI
  • Schwann cell differentiation Source: MGI
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
NGFI-A-binding protein 1
Alternative name(s):
EGR-1-binding protein 1
Gene namesi
Name:Nab1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:107564. Nab1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi33 – 331Q → R: Loss of transcriptional repression; when associated with Q-64. 1 Publication
Mutagenesisi51 – 511E → K: Loss of transcriptional repression. 1 Publication
Mutagenesisi64 – 641H → Q: Loss of transcriptional repression; when associated with R-33. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 486486NGFI-A-binding protein 1PRO_0000077040Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Cross-linki129 – 129Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei171 – 1711PhosphoserineCombined sources
Modified residuei182 – 1821PhosphoserineCombined sources
Modified residuei327 – 3271PhosphoserineBy similarity
Cross-linki332 – 332Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1)By similarity
Cross-linki332 – 332Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki354 – 354Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei405 – 4051PhosphoserineCombined sources
Cross-linki479 – 479Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1)By similarity
Cross-linki479 – 479Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ61122.
MaxQBiQ61122.
PaxDbiQ61122.
PRIDEiQ61122.

PTM databases

iPTMnetiQ61122.
PhosphoSiteiQ61122.

Expressioni

Tissue specificityi

Widely expressed in adult. In day 16 embryo highest levels in forebrain, thymus, salivary gland and cartilage.

Developmental stagei

In day 16 embryo highest levels in forebrain, thymus, salivary gland and cartilage.

Gene expression databases

BgeeiQ61122.
CleanExiMM_NAB1.
ExpressionAtlasiQ61122. baseline and differential.
GenevisibleiQ61122. MM.

Interactioni

Subunit structurei

Homomultimers may associate with EGR1 bound to DNA.

GO - Molecular functioni

  • transcription factor binding Source: MGI

Protein-protein interaction databases

IntActiQ61122. 1 interaction.
MINTiMINT-2737045.
STRINGi10090.ENSMUSP00000066696.

Structurei

3D structure databases

ProteinModelPortaliQ61122.
SMRiQ61122. Positions 181-316.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni4 – 8279NCD1Add
BLAST
Regioni220 – 30990NCD2Add
BLAST
Regioni306 – 33732Necessary for nuclear localizationBy similarityAdd
BLAST

Domaini

The NAB conserved domain 1 (NCD1) interacts with EGR1 inhibitory domain and mediates multimerization.
The NAB conserved domain 2 (NCD2) is necessary for transcriptional repression.

Sequence similaritiesi

Belongs to the NAB family.Curated

Phylogenomic databases

eggNOGiKOG3835. Eukaryota.
ENOG410YKFH. LUCA.
GeneTreeiENSGT00390000006330.
HOGENOMiHOG000026777.
HOVERGENiHBG003127.
InParanoidiQ61122.
OMAiHIFEMSD.
OrthoDBiEOG776SPQ.
TreeFamiTF315501.

Family and domain databases

InterProiIPR006986. Nab1_C.
IPR006989. NAB_co-repressor_dom.
IPR006988. Nab_N.
[Graphical view]
PfamiPF04902. Nab1. 1 hit.
PF04904. NCD1. 1 hit.
PF04905. NCD2. 1 hit.
[Graphical view]
ProDomiPD342192. Nab_N. 1 hit.
[Graphical view] [Entries sharing at least one domain]

Sequencei

Sequence statusi: Complete.

Q61122-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATALPRTLG ELQLYRILQK ANLLSYFDAF IQQGGDDVQQ LCEAGEEEFL
60 70 80 90 100
EIMALVGMAS KPLHVRRLQK ALRDWVTNPG LFNQPLTSLP VSSIPIYKLP
110 120 130 140 150
EGSPTWLGIS CNSYERSSSS REPHLKIPKC AATTCVQSLG QGKSEVGSLA
160 170 180 190 200
LQSVSDSRLW QGHHATESEH SLSPADLGSP ASPKESSEAL DAAAALSVAE
210 220 230 240 250
CVERMAPTLP KSDLSEVKEL LKNNKKLAKM IGHIFEMSDE DPHKEEEIRK
260 270 280 290 300
YSAIYGRFDS KRKDGKHLTL HELTVNEAAA QLCVKDNALL TRRDELFALA
310 320 330 340 350
RQVSREVTYK YTYRTTRLKC GERDELSPKR IKIEDGFPDF QESVPTLFQQ
360 370 380 390 400
ARAKSEELAG LGSQQAEKGM AKQMELLCAQ AGYERLQQER RLTAGLYRQS
410 420 430 440 450
SGEQSPDGGL PSDSSDGQGE RPLNLRIPSV QNRQPHHFVV DGELSRLYSS
460 470 480
EAKSHSSESL GILKDYPHSA FTLEKKVIKT EPEDSR
Length:486
Mass (Da):54,010
Last modified:July 27, 2011 - v2
Checksum:i535CC2ADD604219C
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti181 – 1811A → V in AAC52649 (PubMed:8668170).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U47008 mRNA. Translation: AAC52649.1.
CH466548 Genomic DNA. Translation: EDK99971.1.
BC005627 mRNA. Translation: AAH05627.1.
BC016886 mRNA. Translation: AAH16886.1.
CCDSiCCDS14944.1.
RefSeqiNP_032693.2. NM_008667.3.
XP_006495833.1. XM_006495770.2.
UniGeneiMm.25903.

Genome annotation databases

EnsembliENSMUST00000069792; ENSMUSP00000066696; ENSMUSG00000002881.
GeneIDi17936.
KEGGimmu:17936.
UCSCiuc007ayh.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U47008 mRNA. Translation: AAC52649.1.
CH466548 Genomic DNA. Translation: EDK99971.1.
BC005627 mRNA. Translation: AAH05627.1.
BC016886 mRNA. Translation: AAH16886.1.
CCDSiCCDS14944.1.
RefSeqiNP_032693.2. NM_008667.3.
XP_006495833.1. XM_006495770.2.
UniGeneiMm.25903.

3D structure databases

ProteinModelPortaliQ61122.
SMRiQ61122. Positions 181-316.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ61122. 1 interaction.
MINTiMINT-2737045.
STRINGi10090.ENSMUSP00000066696.

PTM databases

iPTMnetiQ61122.
PhosphoSiteiQ61122.

Proteomic databases

EPDiQ61122.
MaxQBiQ61122.
PaxDbiQ61122.
PRIDEiQ61122.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000069792; ENSMUSP00000066696; ENSMUSG00000002881.
GeneIDi17936.
KEGGimmu:17936.
UCSCiuc007ayh.2. mouse.

Organism-specific databases

CTDi4664.
MGIiMGI:107564. Nab1.

Phylogenomic databases

eggNOGiKOG3835. Eukaryota.
ENOG410YKFH. LUCA.
GeneTreeiENSGT00390000006330.
HOGENOMiHOG000026777.
HOVERGENiHBG003127.
InParanoidiQ61122.
OMAiHIFEMSD.
OrthoDBiEOG776SPQ.
TreeFamiTF315501.

Miscellaneous databases

PROiQ61122.
SOURCEiSearch...

Gene expression databases

BgeeiQ61122.
CleanExiMM_NAB1.
ExpressionAtlasiQ61122. baseline and differential.
GenevisibleiQ61122. MM.

Family and domain databases

InterProiIPR006986. Nab1_C.
IPR006989. NAB_co-repressor_dom.
IPR006988. Nab_N.
[Graphical view]
PfamiPF04902. Nab1. 1 hit.
PF04904. NCD1. 1 hit.
PF04905. NCD2. 1 hit.
[Graphical view]
ProDomiPD342192. Nab_N. 1 hit.
[Graphical view] [Entries sharing at least one domain]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Identification of NAB1, a repressor of NGFI-A- and Krox20-mediated transcription."
    Russo M.W., Sevetson B.R., Milbrandt J.
    Proc. Natl. Acad. Sci. U.S.A. 92:6873-6877(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Brain.
  2. "NAB2, a corepressor of NGFI-A (Egr-1) and Krox20, is induced by proliferative and differentiative stimuli."
    Svaren J., Sevetson B.R., Apel E.D., Zimonjic D.B., Popescu N.C., Milbrandt J.
    Mol. Cell. Biol. 16:3545-3553(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Brain.
  3. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: 129 and FVB/N.
    Tissue: Kidney and Mammary tumor.
  5. "Novel mutants of NAB corepressors enhance activation by Egr transactivators."
    Svaren J., Sevetson B.R., Golda T., Stanton J.J., Swirnoff A.H., Milbrandt J.
    EMBO J. 17:6010-6019(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, MUTAGENESIS.
  6. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.
  7. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-171; SER-182 AND SER-405, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Kidney and Testis.

Entry informationi

Entry nameiNAB1_MOUSE
AccessioniPrimary (citable) accession number: Q61122
Secondary accession number(s): Q99J24
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: July 27, 2011
Last modified: June 8, 2016
This is version 117 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.