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Protein

NGFI-A-binding protein 1

Gene

Nab1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Acts as a transcriptional repressor for zinc finger transcription factors EGR1 and EGR2.By similarity1 Publication

GO - Molecular functioni

  • transcription factor binding Source: MGI

GO - Biological processi

  • endochondral ossification Source: MGI
  • myelination Source: MGI
  • negative regulation of transcription, DNA-templated Source: InterPro
  • regulation of epidermis development Source: MGI
  • regulation of transcription, DNA-templated Source: MGI
  • Schwann cell differentiation Source: MGI
  • transcription, DNA-templated Source: UniProtKB-KW

Keywordsi

Molecular functionRepressor
Biological processTranscription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
NGFI-A-binding protein 1
Alternative name(s):
EGR-1-binding protein 1
Gene namesi
Name:Nab1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:107564. Nab1.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi33Q → R: Loss of transcriptional repression; when associated with Q-64. 1 Publication1
Mutagenesisi51E → K: Loss of transcriptional repression. 1 Publication1
Mutagenesisi64H → Q: Loss of transcriptional repression; when associated with R-33. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000770401 – 486NGFI-A-binding protein 1Add BLAST486

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki126Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki129Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki143Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei171PhosphoserineCombined sources1
Modified residuei182PhosphoserineCombined sources1
Cross-linki211Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei327PhosphoserineBy similarity1
Cross-linki332Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1)By similarity
Cross-linki332Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki354Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki368Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki372Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei405PhosphoserineCombined sources1
Cross-linki453Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki464Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki476Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki479Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1)By similarity
Cross-linki479Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ61122.
MaxQBiQ61122.
PaxDbiQ61122.
PRIDEiQ61122.

PTM databases

iPTMnetiQ61122.
PhosphoSitePlusiQ61122.

Expressioni

Tissue specificityi

Widely expressed in adult. In day 16 embryo highest levels in forebrain, thymus, salivary gland and cartilage.

Developmental stagei

In day 16 embryo highest levels in forebrain, thymus, salivary gland and cartilage.

Gene expression databases

BgeeiENSMUSG00000002881.
CleanExiMM_NAB1.
ExpressionAtlasiQ61122. baseline and differential.
GenevisibleiQ61122. MM.

Interactioni

Subunit structurei

Homomultimers may associate with EGR1 bound to DNA.

GO - Molecular functioni

  • transcription factor binding Source: MGI

Protein-protein interaction databases

IntActiQ61122. 1 interactor.
MINTiMINT-2737045.
STRINGi10090.ENSMUSP00000066696.

Structurei

3D structure databases

ProteinModelPortaliQ61122.
SMRiQ61122.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni4 – 82NCD1Add BLAST79
Regioni220 – 309NCD2Add BLAST90
Regioni306 – 337Necessary for nuclear localizationBy similarityAdd BLAST32

Domaini

The NAB conserved domain 1 (NCD1) interacts with EGR1 inhibitory domain and mediates multimerization.
The NAB conserved domain 2 (NCD2) is necessary for transcriptional repression.

Sequence similaritiesi

Belongs to the NAB family.Curated

Phylogenomic databases

eggNOGiKOG3835. Eukaryota.
ENOG410YKFH. LUCA.
GeneTreeiENSGT00390000006330.
HOGENOMiHOG000026777.
HOVERGENiHBG003127.
InParanoidiQ61122.
OMAiAAAQLCM.
OrthoDBiEOG091G0NQE.
TreeFamiTF315501.

Family and domain databases

InterProiView protein in InterPro
IPR006986. Nab1_C.
IPR006989. NAB_co-repressor_dom.
IPR006988. Nab_N.
PfamiView protein in Pfam
PF04902. Nab1. 1 hit.
PF04904. NCD1. 1 hit.
PF04905. NCD2. 1 hit.
ProDomiView protein in ProDom or Entries sharing at least one domain
PD342192. Nab_N. 1 hit.

Sequencei

Sequence statusi: Complete.

Q61122-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATALPRTLG ELQLYRILQK ANLLSYFDAF IQQGGDDVQQ LCEAGEEEFL
60 70 80 90 100
EIMALVGMAS KPLHVRRLQK ALRDWVTNPG LFNQPLTSLP VSSIPIYKLP
110 120 130 140 150
EGSPTWLGIS CNSYERSSSS REPHLKIPKC AATTCVQSLG QGKSEVGSLA
160 170 180 190 200
LQSVSDSRLW QGHHATESEH SLSPADLGSP ASPKESSEAL DAAAALSVAE
210 220 230 240 250
CVERMAPTLP KSDLSEVKEL LKNNKKLAKM IGHIFEMSDE DPHKEEEIRK
260 270 280 290 300
YSAIYGRFDS KRKDGKHLTL HELTVNEAAA QLCVKDNALL TRRDELFALA
310 320 330 340 350
RQVSREVTYK YTYRTTRLKC GERDELSPKR IKIEDGFPDF QESVPTLFQQ
360 370 380 390 400
ARAKSEELAG LGSQQAEKGM AKQMELLCAQ AGYERLQQER RLTAGLYRQS
410 420 430 440 450
SGEQSPDGGL PSDSSDGQGE RPLNLRIPSV QNRQPHHFVV DGELSRLYSS
460 470 480
EAKSHSSESL GILKDYPHSA FTLEKKVIKT EPEDSR
Length:486
Mass (Da):54,010
Last modified:July 27, 2011 - v2
Checksum:i535CC2ADD604219C
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti181A → V in AAC52649 (PubMed:8668170).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U47008 mRNA. Translation: AAC52649.1.
CH466548 Genomic DNA. Translation: EDK99971.1.
BC005627 mRNA. Translation: AAH05627.1.
BC016886 mRNA. Translation: AAH16886.1.
CCDSiCCDS14944.1.
RefSeqiNP_032693.2. NM_008667.3.
XP_006495833.1. XM_006495770.2.
UniGeneiMm.25903.

Genome annotation databases

EnsembliENSMUST00000069792; ENSMUSP00000066696; ENSMUSG00000002881.
GeneIDi17936.
KEGGimmu:17936.
UCSCiuc007ayh.2. mouse.

Similar proteinsi

Entry informationi

Entry nameiNAB1_MOUSE
AccessioniPrimary (citable) accession number: Q61122
Secondary accession number(s): Q99J24
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: July 27, 2011
Last modified: August 30, 2017
This is version 127 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families