Reviewed, UniProtKB/Swiss-Prot Q61122 (NAB1_MOUSE)

Names and origin

Protein names

Recommended name:
    NGFI-A-binding protein 1
Alternative name(s):
    EGR-1-binding protein 1

Gene names

Name:

Nab1

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	486 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Acts as a transcriptional repressor for zinc finger transcription factors EGR1 and EGR2 By similarity.
Subunit structure	Homomultimers may associate with EGR1 bound to DNA.
Subcellular location	Nucleus By similarity.
Tissue specificity	Widely expressed in adult. In day 16 embryo highest levels in forebrain, thymus, salivary gland and cartilage.
Developmental stage	In day 16 embryo highest levels in forebrain, thymus, salivary gland and cartilage.
Domain	The NAB conserved domain 1 (NCD1) interacts with EGR1 inhibitory domain and mediates multimerization. The NAB conserved domain 2 (NCD2) is necessary for transcriptional repression.
Sequence similarities	Belongs to the NAB family.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Molecular function	Repressor
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	Schwann cell differentiation Inferred from genetic interaction. Source: MGI endochondral ossification Inferred from genetic interaction. Source: MGI myelination Inferred from genetic interaction. Source: MGI negative regulation of transcription Inferred from electronic annotation. Source: InterPro regulation of epidermis development Inferred from genetic interaction. Source: MGI regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transcription Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	transcription repressor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 486	486	NGFI-A-binding protein 1		PRO_0000077040
Regions
	Region	4 – 82	79	NCD1
	Region	220 – 309	90	NCD2
	Region	306 – 337	32	Necessary for nuclear localization By similarity
Amino acid modifications
	Modified residue	327	1	Phosphoserine By similarity
	Modified residue	401	1	Phosphoserine Ref.4
Experimental info
	Mutagenesis	33	1	Q → R: Loss of transcriptional repression; when associated with Q-64.
	Mutagenesis	51	1	E → K: Loss of transcriptional repression.
	Mutagenesis	64	1	H → Q: Loss of transcriptional repression; when associated with R-33.

Sequences

	Sequence		Length	Mass (Da)
	Q61122-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: FB42E00B0B551B2A	FASTA	486	54,038
	10 20 30 40 50 60 MATALPRTLG ELQLYRILQK ANLLSYFDAF IQQGGDDVQQ LCEAGEEEFL EIMALVGMAS 70 80 90 100 110 120 KPLHVRRLQK ALRDWVTNPG LFNQPLTSLP VSSIPIYKLP EGSPTWLGIS CNSYERSSSS 130 140 150 160 170 180 REPHLKIPKC AATTCVQSLG QGKSEVGSLA LQSVSDSRLW QGHHATESEH SLSPADLGSP 190 200 210 220 230 240 VSPKESSEAL DAAAALSVAE CVERMAPTLP KSDLSEVKEL LKNNKKLAKM IGHIFEMSDE 250 260 270 280 290 300 DPHKEEEIRK YSAIYGRFDS KRKDGKHLTL HELTVNEAAA QLCVKDNALL TRRDELFALA 310 320 330 340 350 360 RQVSREVTYK YTYRTTRLKC GERDELSPKR IKIEDGFPDF QESVPTLFQQ ARAKSEELAG 370 380 390 400 410 420 LGSQQAEKGM AKQMELLCAQ AGYERLQQER RLTAGLYRQS SGEQSPDGGL PSDSSDGQGE 430 440 450 460 470 480 RPLNLRIPSV QNRQPHHFVV DGELSRLYSS EAKSHSSESL GILKDYPHSA FTLEKKVIKT EPEDSR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification of NAB1, a repressor of NGFI-A- and Krox20-mediated transcription." Russo M.W., Sevetson B.R., Milbrandt J. Proc. Natl. Acad. Sci. U.S.A. 92:6873-6877(1995) [PubMed: 7624335] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Brain.
[2]	"NAB2, a corepressor of NGFI-A (Egr-1) and Krox20, is induced by proliferative and differentiative stimuli." Svaren J., Sevetson B.R., Apel E.D., Zimonjic D.B., Popescu N.C., Milbrandt J. Mol. Cell. Biol. 16:3545-3553(1996) [PubMed: 8668170] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Brain.
[3]	"Novel mutants of NAB corepressors enhance activation by Egr transactivators." Svaren J., Sevetson B.R., Golda T., Stanton J.J., Swirnoff A.H., Milbrandt J. EMBO J. 17:6010-6019(1998) [PubMed: 9774344] [Abstract] Cited for: FUNCTION, MUTAGENESIS.
[4]	"Large-scale phosphorylation analysis of mouse liver." Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed: 17242355] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-401, MASS SPECTROMETRY. Tissue: Liver.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U47008 mRNA. Translation: AAC52649.1.
IPI	IPI00408414.
UniGene	Mm.25903
3D structure databases
ModBase	Search...
PTM databases
PhosphoSite	Q61122.
Genome annotation databases
Ensembl	ENSMUSG00000002881. Mus musculus. [Contig view]
Organism-specific databases
MGI	MGI:107564. Nab1.
Phylogenomic databases
HOGENOM	Q61122.
HOVERGEN	Q61122.
Gene expression databases
ArrayExpress	Q61122.
Bgee	Q61122.
CleanEx	MM_NAB1.
GermOnline	ENSMUSG00000002881. Mus musculus.
Family and domain databases
InterPro	IPR006986. Nab1_C. IPR006989. Nab_centr. IPR006988. Nab_N. [Graphical view]
Pfam	PF04902. Nab1. 1 hit. PF04904. NCD1. 1 hit. PF04905. NCD2. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
SOURCE	Search...

Entry information

Entry name

NAB1_MOUSE

Accession

Primary (citable) accession number: Q61122

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	November 1, 1996
Last modified:	June 16, 2009
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)