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Q61115 (PTC1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 117. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Protein patched homolog 1

Short name=PTC
Short name=PTC1
Gene names
Name:Ptch1
Synonyms:Ptch
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length1434 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Acts as a receptor for sonic hedgehog (SHH), indian hedgehog (IHH) and desert hedgehog (DHH). Associates with the smoothened protein (SMO) to transduce the hedgehog's proteins signal. Seems to have a tumor suppressor function, as inactivation of this protein is probably a necessary, if not sufficient step for tumorigenesis. Ref.2

Subunit structure

Interacts with SNX17 By similarity. Interacts with IHH. Ref.2

Subcellular location

Membrane; Multi-pass membrane protein.

Developmental stage

Expressed at low level at 7 dpc, higher level at 11 and 15 dpc, and persists at moderate levels at 17 dpc. In the developing embryo, PTC is first detected within the ventral neural tube and later in the somites and limb buds. Expression in the limb buds is restricted to the posterior ectoderm surrounding the zone of polarizing activity. In the adult, expression is seen in brain, lung, liver, kidney and ocular tissues; lower levels in heart, skeletal muscle, and testis.

Induction

Activated by Sonic hedgehog.

Post-translational modification

Glycosylation is necessary for SHH binding By similarity.

Sequence similarities

Belongs to the patched family.

Contains 1 SSD (sterol-sensing) domain.

Ontologies

Keywords
   Cellular componentMembrane
   DomainTransmembrane
Transmembrane helix
   Molecular functionReceptor
   PTMGlycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processbrain development

Inferred from mutant phenotype PubMed 11517919. Source: BHF-UCL

branching involved in ureteric bud morphogenesis

Inferred from mutant phenotype PubMed 19809516. Source: MGI

cell differentiation involved in kidney development

Inferred from genetic interaction PubMed 19809516. Source: MGI

cell proliferation involved in metanephros development

Inferred from mutant phenotype PubMed 19809516. Source: MGI

cellular response to cholesterol

Inferred from expression pattern PubMed 21931618. Source: BHF-UCL

dorsal/ventral neural tube patterning

Inferred from mutant phenotype PubMed 9262482. Source: MGI

dorsal/ventral pattern formation

Inferred from genetic interaction PubMed 15576403. Source: MGI

embryonic limb morphogenesis

Inferred from mutant phenotype PubMed 11784021. Source: MGI

embryonic organ development

Inferred from genetic interaction PubMed 24302887. Source: MGI

epidermis development

Inferred from mutant phenotype PubMed 17631878. Source: MGI

glucose homeostasis

Inferred from mutant phenotype PubMed 11044404. Source: MGI

heart morphogenesis

Inferred from mutant phenotype PubMed 9262482. Source: MGI

hindlimb morphogenesis

Inferred from mutant phenotype PubMed 9262482. Source: MGI

keratinocyte proliferation

Inferred from mutant phenotype PubMed 17631878. Source: MGI

mammary gland development

Inferred from mutant phenotype PubMed 10529434. Source: MGI

mammary gland duct morphogenesis

Inferred from mutant phenotype PubMed 10529434. Source: MGI

mammary gland epithelial cell differentiation

Inferred from mutant phenotype PubMed 10529434. Source: MGI

negative regulation of cell division

Inferred from mutant phenotype PubMed 11331587. Source: BHF-UCL

negative regulation of cell proliferation

Inferred from mutant phenotype PubMed 11331587. Source: BHF-UCL

negative regulation of epithelial cell proliferation

Inferred from mutant phenotype PubMed 17631878. Source: MGI

negative regulation of multicellular organism growth

Inferred from mutant phenotype PubMed 11784021. Source: MGI

negative regulation of osteoblast differentiation

Inferred from electronic annotation. Source: Ensembl

negative regulation of sequence-specific DNA binding transcription factor activity

Inferred from electronic annotation. Source: Ensembl

negative regulation of smoothened signaling pathway

Inferred from genetic interaction PubMed 21931618. Source: BHF-UCL

negative regulation of transcription from RNA polymerase II promoter

Inferred from electronic annotation. Source: Ensembl

neural plate axis specification

Inferred from mutant phenotype PubMed 11517919. Source: BHF-UCL

neural tube closure

Inferred from mutant phenotype PubMed 9262482. Source: MGI

neural tube formation

Inferred from direct assay PubMed 10500113. Source: Roslin

organ morphogenesis

Inferred from mutant phenotype PubMed 12917290. Source: MGI

pattern specification process

Inferred from mutant phenotype PubMed 9262482. Source: MGI

pharyngeal system development

Inferred from electronic annotation. Source: Ensembl

positive regulation of cholesterol efflux

Inferred from electronic annotation. Source: Ensembl

protein processing

Inferred from mutant phenotype PubMed 16061793. Source: MGI

protein targeting to plasma membrane

Inferred from electronic annotation. Source: Ensembl

regulation of cell proliferation

Inferred from mutant phenotype PubMed 19809516. Source: MGI

regulation of growth

Inferred from mutant phenotype PubMed 9262482. Source: MGI

regulation of mitotic cell cycle

Inferred from mutant phenotype PubMed 16489008. Source: MGI

regulation of protein localization

Inferred from direct assay PubMed 11331587. Source: BHF-UCL

regulation of smoothened signaling pathway

Inferred from mutant phenotype PubMed 11784021. Source: MGI

response to chlorate

Inferred from direct assay PubMed 11476578. Source: MGI

response to drug

Inferred from electronic annotation. Source: Ensembl

response to estradiol

Inferred from electronic annotation. Source: Ensembl

response to mechanical stimulus

Inferred from electronic annotation. Source: Ensembl

response to retinoic acid

Inferred from electronic annotation. Source: Ensembl

signal transduction

Inferred from direct assay PubMed 10500113. Source: Roslin

smoothened signaling pathway

Inferred from direct assay PubMed 11493558PubMed 9006067. Source: MGI

smoothened signaling pathway involved in dorsal/ventral neural tube patterning

Inferred from mutant phenotype PubMed 19684112. Source: MGI

somite development

Inferred from mutant phenotype PubMed 11517919. Source: BHF-UCL

spinal cord motor neuron differentiation

Inferred from genetic interaction PubMed 21552265. Source: MGI

   Cellular_componentGolgi apparatus

Inferred from electronic annotation. Source: Ensembl

axonal growth cone

Inferred from electronic annotation. Source: Ensembl

caveola

Inferred from electronic annotation. Source: Ensembl

dendritic growth cone

Inferred from electronic annotation. Source: Ensembl

extracellular region

Traceable author statement PubMed 10500113. Source: Roslin

integral component of plasma membrane

Traceable author statement PubMed 11784021. Source: MGI

midbody

Inferred from direct assay PubMed 15166316. Source: UniProtKB

perinuclear region of cytoplasm

Inferred from electronic annotation. Source: Ensembl

plasma membrane

Inferred from direct assay PubMed 11331587PubMed 21931618. Source: BHF-UCL

postsynaptic density

Inferred from electronic annotation. Source: Ensembl

primary cilium

Inferred from direct assay PubMed 19654211PubMed 19684112. Source: MGI

   Molecular_functioncholesterol binding

Traceable author statement PubMed 10500113. Source: Roslin

cyclin binding

Inferred from physical interaction PubMed 11331587. Source: BHF-UCL

hedgehog receptor activity

Traceable author statement PubMed 11784021. Source: MGI

heparin binding

Inferred from direct assay PubMed 10500113. Source: Roslin

patched binding

Inferred from direct assay PubMed 10500113. Source: Roslin

protein binding

Inferred from physical interaction PubMed 10500113Ref.2. Source: UniProtKB

protein complex binding

Inferred from direct assay PubMed 11331587. Source: BHF-UCL

zinc ion binding

Non-traceable author statement PubMed 10500113. Source: Roslin

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 14341434Protein patched homolog 1
PRO_0000205965

Regions

Topological domain1 – 8686Cytoplasmic Potential
Transmembrane87 – 10721Helical; Potential
Topological domain108 – 422315Extracellular Potential
Transmembrane423 – 44321Helical; Potential
Topological domain444 – 45815Cytoplasmic Potential
Transmembrane459 – 47921Helical; Potential
Topological domain480 – 4878Extracellular Potential
Transmembrane488 – 50821Helical; Potential
Topological domain509 – 53325Cytoplasmic Potential
Transmembrane534 – 55421Helical; Potential
Topological domain555 – 5639Extracellular Potential
Transmembrane564 – 58421Helical; Potential
Topological domain585 – 734150Cytoplasmic Potential
Transmembrane735 – 75521Helical; Potential
Topological domain756 – 1013258Extracellular Potential
Transmembrane1014 – 103421Helical; Potential
Topological domain1035 – 10395Cytoplasmic Potential
Transmembrane1040 – 106021Helical; Potential
Topological domain1061 – 10699Extracellular Potential
Transmembrane1070 – 109021Helical; Potential
Topological domain1091 – 110717Cytoplasmic Potential
Transmembrane1108 – 112821Helical; Potential
Topological domain1129 – 114012Extracellular Potential
Transmembrane1141 – 116121Helical; Potential
Topological domain1162 – 1434273Cytoplasmic Potential
Domain424 – 584161SSD

Amino acid modifications

Glycosylation1271N-linked (GlcNAc...) Potential
Glycosylation2981N-linked (GlcNAc...) Potential
Glycosylation3351N-linked (GlcNAc...) Potential
Glycosylation4001N-linked (GlcNAc...) Potential
Glycosylation8611N-linked (GlcNAc...) Potential
Glycosylation9861N-linked (GlcNAc...) Potential

Sequences

Sequence LengthMass (Da)Tools
Q61115 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: A5E9189E633173D0

FASTA1,434159,273
        10         20         30         40         50         60 
MASAGNAAGA LGRQAGGGRR RRTGGPHRAA PDRDYLHRPS YCDAAFALEQ ISKGKATGRK 

        70         80         90        100        110        120 
APLWLRAKFQ RLLFKLGCYI QKNCGKFLVV GLLIFGAFAV GLKAANLETN VEELWVEVGG 

       130        140        150        160        170        180 
RVSRELNYTR QKIGEEAMFN PQLMIQTPKE EGANVLTTEA LLQHLDSALQ ASRVHVYMYN 

       190        200        210        220        230        240 
RQWKLEHLCY KSGELITETG YMDQIIEYLY PCLIITPLDC FWEGAKLQSG TAYLLGKPPL 

       250        260        270        280        290        300 
RWTNFDPLEF LEELKKINYQ VDSWEEMLNK AEVGHGYMDR PCLNPADPDC PATAPNKNST 

       310        320        330        340        350        360 
KPLDVALVLN GGCQGLSRKY MHWQEELIVG GTVKNATGKL VSAHALQTMF QLMTPKQMYE 

       370        380        390        400        410        420 
HFRGYDYVSH INWNEDRAAA ILEAWQRTYV EVVHQSVAPN STQKVLPFTT TTLDDILKSF 

       430        440        450        460        470        480 
SDVSVIRVAS GYLLMLAYAC LTMLRWDCSK SQGAVGLAGV LLVALSVAAG LGLCSLIGIS 

       490        500        510        520        530        540 
FNAATTQVLP FLALGVGVDD VFLLAHAFSE TGQNKRIPFE DRTGECLKRT GASVALTSIS 

       550        560        570        580        590        600 
NVTAFFMAAL IPIPALRAFS LQAAVVVVFN FAMVLLIFPA ILSMDLYRRE DRRLDIFCCF 

       610        620        630        640        650        660 
TSPCVSRVIQ VEPQAYTEPH SNTRYSPPPP YTSHSFAHET HITMQSTVQL RTEYDPHTHV 

       670        680        690        700        710        720 
YYTTAEPRSE ISVQPVTVTQ DNLSCQSPES TSSTRDLLSQ FSDSSLHCLE PPCTKWTLSS 

       730        740        750        760        770        780 
FAEKHYAPFL LKPKAKVVVI LLFLGLLGVS LYGTTRVRDG LDLTDIVPRE TREYDFIAAQ 

       790        800        810        820        830        840 
FKYFSFYNMY IVTQKADYPN IQHLLYDLHK SFSNVKYVML EENKQLPQMW LHYFRDWLQG 

       850        860        870        880        890        900 
LQDAFDSDWE TGRIMPNNYK NGSDDGVLAY KLLVQTGSRD KPIDISQLTK QRLVDADGII 

       910        920        930        940        950        960 
NPSAFYIYLT AWVSNDPVAY AASQANIRPH RPEWVHDKAD YMPETRLRIP AAEPIEYAQF 

       970        980        990       1000       1010       1020 
PFYLNGLRDT SDFVEAIEKV RVICNNYTSL GLSSYPNGYP FLFWEQYISL RHWLLLSISV 

      1030       1040       1050       1060       1070       1080 
VLACTFLVCA VFLLNPWTAG IIVMVLALMT VELFGMMGLI GIKLSAVPVV ILIASVGIGV 

      1090       1100       1110       1120       1130       1140 
EFTVHVALAF LTAIGDKNHR AMLALEHMFA PVLDGAVSTL LGVLMLAGSE FDFIVRYFFA 

      1150       1160       1170       1180       1190       1200 
VLAILTVLGV LNGLVLLPVL LSFFGPCPEV SPANGLNRLP TPSPEPPPSV VRFAVPPGHT 

      1210       1220       1230       1240       1250       1260 
NNGSDSSDSE YSSQTTVSGI SEELRQYEAQ QGAGGPAHQV IVEATENPVF ARSTVVHPDS 

      1270       1280       1290       1300       1310       1320 
RHQPPLTPRQ QPHLDSGSLS PGRQGQQPRR DPPREGLRPP PYRPRRDAFE ISTEGHSGPS 

      1330       1340       1350       1360       1370       1380 
NRDRSGPRGA RSHNPRNPTS TAMGSSVPSY CQPITTVTAS ASVTVAVHPP PGPGRNPRGG 

      1390       1400       1410       1420       1430 
PCPGYESYPE TDHGVFEDPH VPFHVRCERR DSKVEVIELQ DVECEERPWG SSSN 

« Hide

References

[1]"Conservation of the hedgehog/patched signaling pathway from flies to mice: induction of a mouse patched gene by Hedgehog."
Goodrich L.V., Johnson R.L., Milenkovic L., McMahon J.A., Scott M.P.
Genes Dev. 10:301-312(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Embryo.
[2]"Indian hedgehog mutations causing brachydactyly type A1 impair Hedgehog signal transduction at multiple levels."
Ma G., Yu J., Xiao Y., Chan D., Gao B., Hu J., He Y., Guo S., Zhou J., Zhang L., Gao L., Zhang W., Kang Y., Cheah K.S., Feng G., Guo X., Wang Y., Zhou C.Z., He L.
Cell Res. 21:1343-1357(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH IHH.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U46155 mRNA. Translation: AAC98798.1.
CCDSCCDS26592.1.
PIRT30172.
RefSeqNP_032983.1. NM_008957.2.
XP_006517222.1. XM_006517159.1.
UniGeneMm.228798.
Mm.488634.

3D structure databases

ProteinModelPortalQ61115.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

DIPDIP-60268N.

PTM databases

PhosphoSiteQ61115.

Proteomic databases

PRIDEQ61115.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000021921; ENSMUSP00000021921; ENSMUSG00000021466.
GeneID19206.
KEGGmmu:19206.
UCSCuc007qxv.1. mouse.

Organism-specific databases

CTD5727.
MGIMGI:105373. Ptch1.

Phylogenomic databases

eggNOGNOG313603.
GeneTreeENSGT00680000099777.
HOGENOMHOG000231331.
HOVERGENHBG003801.
InParanoidQ61115.
KOK06225.
OMAPDRDYLH.
OrthoDBEOG7HMS06.
PhylomeDBQ61115.
TreeFamTF106489.

Enzyme and pathway databases

ReactomeREACT_188257. Signal Transduction.

Gene expression databases

ArrayExpressQ61115.
BgeeQ61115.
CleanExMM_PTCH1.
GenevestigatorQ61115.

Family and domain databases

InterProIPR003392. Patched.
IPR000731. SSD.
IPR004766. TM_rcpt_patched.
[Graphical view]
PfamPF02460. Patched. 1 hit.
[Graphical view]
TIGRFAMsTIGR00918. 2A060602. 1 hit.
PROSITEPS50156. SSD. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio295938.
PROQ61115.
SOURCESearch...

Entry information

Entry namePTC1_MOUSE
AccessionPrimary (citable) accession number: Q61115
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 1, 1996
Last modified: July 9, 2014
This is version 117 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot