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Protein

Protein patched homolog 1

Gene

Ptch1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Acts as a receptor for sonic hedgehog (SHH), indian hedgehog (IHH) and desert hedgehog (DHH). Associates with the smoothened protein (SMO) to transduce the hedgehog's proteins signal. Seems to have a tumor suppressor function, as inactivation of this protein is probably a necessary, if not sufficient step for tumorigenesis.1 Publication

GO - Molecular functioni

  • cholesterol binding Source: Roslin
  • cyclin binding Source: BHF-UCL
  • hedgehog family protein binding Source: MGI
  • hedgehog receptor activity Source: MGI
  • heparin binding Source: Roslin
  • patched binding Source: Roslin
  • protein complex binding Source: BHF-UCL
  • smoothened binding Source: MGI
  • zinc ion binding Source: Roslin

GO - Biological processi

  • animal organ morphogenesis Source: MGI
  • brain development Source: BHF-UCL
  • branching involved in ureteric bud morphogenesis Source: MGI
  • cell differentiation involved in kidney development Source: MGI
  • cell fate determination Source: MGI
  • cell proliferation involved in metanephros development Source: MGI
  • cellular response to cholesterol Source: BHF-UCL
  • dorsal/ventral neural tube patterning Source: MGI
  • dorsal/ventral pattern formation Source: MGI
  • embryonic limb morphogenesis Source: MGI
  • embryonic organ development Source: MGI
  • epidermal cell fate specification Source: MGI
  • epidermis development Source: MGI
  • glucose homeostasis Source: MGI
  • heart morphogenesis Source: MGI
  • hindlimb morphogenesis Source: MGI
  • in utero embryonic development Source: MGI
  • keratinocyte proliferation Source: MGI
  • limb morphogenesis Source: MGI
  • mammary gland development Source: MGI
  • mammary gland duct morphogenesis Source: MGI
  • mammary gland epithelial cell differentiation Source: MGI
  • negative regulation of cell division Source: BHF-UCL
  • negative regulation of cell proliferation Source: BHF-UCL
  • negative regulation of epithelial cell proliferation Source: MGI
  • negative regulation of multicellular organism growth Source: MGI
  • negative regulation of osteoblast differentiation Source: MGI
  • negative regulation of sequence-specific DNA binding transcription factor activity Source: MGI
  • negative regulation of smoothened signaling pathway Source: BHF-UCL
  • negative regulation of transcription, DNA-templated Source: CACAO
  • negative regulation of transcription from RNA polymerase II promoter Source: MGI
  • neural plate axis specification Source: BHF-UCL
  • neural tube closure Source: MGI
  • neural tube formation Source: Roslin
  • neural tube patterning Source: MGI
  • pattern specification process Source: MGI
  • pharyngeal system development Source: MGI
  • positive regulation of cholesterol efflux Source: MGI
  • positive regulation of epidermal cell differentiation Source: MGI
  • positive regulation of transcription, DNA-templated Source: CACAO
  • protein processing Source: MGI
  • protein targeting to plasma membrane Source: MGI
  • regulation of cell proliferation Source: MGI
  • regulation of growth Source: MGI
  • regulation of mitotic cell cycle Source: MGI
  • regulation of protein localization Source: BHF-UCL
  • regulation of smoothened signaling pathway Source: MGI
  • response to chlorate Source: MGI
  • response to drug Source: Ensembl
  • response to estradiol Source: Ensembl
  • response to mechanical stimulus Source: Ensembl
  • response to retinoic acid Source: Ensembl
  • signal transduction Source: Roslin
  • smoothened signaling pathway Source: MGI
  • smoothened signaling pathway involved in dorsal/ventral neural tube patterning Source: MGI
  • somite development Source: BHF-UCL
  • spinal cord motor neuron differentiation Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Receptor

Enzyme and pathway databases

ReactomeiR-MMU-5610787. Hedgehog 'off' state.
R-MMU-5632681. Ligand-receptor interactions.
R-MMU-5632684. Hedgehog 'on' state.
R-MMU-5635838. Activation of SMO.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein patched homolog 1
Short name:
PTC
Short name:
PTC1
Gene namesi
Name:Ptch1
Synonyms:Ptch
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 13

Organism-specific databases

MGIiMGI:105373. Ptch1.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 86CytoplasmicSequence analysisAdd BLAST86
Transmembranei87 – 107HelicalSequence analysisAdd BLAST21
Topological domaini108 – 422ExtracellularSequence analysisAdd BLAST315
Transmembranei423 – 443HelicalSequence analysisAdd BLAST21
Topological domaini444 – 458CytoplasmicSequence analysisAdd BLAST15
Transmembranei459 – 479HelicalSequence analysisAdd BLAST21
Topological domaini480 – 487ExtracellularSequence analysis8
Transmembranei488 – 508HelicalSequence analysisAdd BLAST21
Topological domaini509 – 533CytoplasmicSequence analysisAdd BLAST25
Transmembranei534 – 554HelicalSequence analysisAdd BLAST21
Topological domaini555 – 563ExtracellularSequence analysis9
Transmembranei564 – 584HelicalSequence analysisAdd BLAST21
Topological domaini585 – 734CytoplasmicSequence analysisAdd BLAST150
Transmembranei735 – 755HelicalSequence analysisAdd BLAST21
Topological domaini756 – 1013ExtracellularSequence analysisAdd BLAST258
Transmembranei1014 – 1034HelicalSequence analysisAdd BLAST21
Topological domaini1035 – 1039CytoplasmicSequence analysis5
Transmembranei1040 – 1060HelicalSequence analysisAdd BLAST21
Topological domaini1061 – 1069ExtracellularSequence analysis9
Transmembranei1070 – 1090HelicalSequence analysisAdd BLAST21
Topological domaini1091 – 1107CytoplasmicSequence analysisAdd BLAST17
Transmembranei1108 – 1128HelicalSequence analysisAdd BLAST21
Topological domaini1129 – 1140ExtracellularSequence analysisAdd BLAST12
Transmembranei1141 – 1161HelicalSequence analysisAdd BLAST21
Topological domaini1162 – 1434CytoplasmicSequence analysisAdd BLAST273

GO - Cellular componenti

  • axonal growth cone Source: Ensembl
  • caveola Source: MGI
  • cilium Source: MGI
  • dendritic growth cone Source: Ensembl
  • extracellular region Source: Roslin
  • Golgi apparatus Source: Ensembl
  • integral component of plasma membrane Source: MGI
  • intracellular membrane-bounded organelle Source: MGI
  • midbody Source: UniProtKB
  • perinuclear region of cytoplasm Source: MGI
  • plasma membrane Source: BHF-UCL
  • postsynaptic density Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi1413K → R: Accumulates at the plasma membrane, increased half-life and increased CASP9-mediated cell death. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002059651 – 1434Protein patched homolog 1Add BLAST1434

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi127N-linked (GlcNAc...)Sequence analysis1
Glycosylationi298N-linked (GlcNAc...)Sequence analysis1
Glycosylationi335N-linked (GlcNAc...)Sequence analysis1
Glycosylationi400N-linked (GlcNAc...)Sequence analysis1
Glycosylationi861N-linked (GlcNAc...)Sequence analysis1
Glycosylationi986N-linked (GlcNAc...)Sequence analysis1
Modified residuei1181PhosphothreonineCombined sources1
Modified residuei1183PhosphoserineCombined sources1
Cross-linki1413Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication

Post-translational modificationi

Glycosylation is necessary for SHH binding.By similarity
In the absence of Hh ligands, ubiquitination by ITCH at Lys-1413 promotes endocytosis and both proteasomal and lysosomal degradation.1 Publication

Keywords - PTMi

Glycoprotein, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ61115.
PRIDEiQ61115.

PTM databases

iPTMnetiQ61115.
PhosphoSitePlusiQ61115.

Expressioni

Developmental stagei

Expressed at low level at 7 dpc, higher level at 11 and 15 dpc, and persists at moderate levels at 17 dpc. In the developing embryo, PTC is first detected within the ventral neural tube and later in the somites and limb buds. Expression in the limb buds is restricted to the posterior ectoderm surrounding the zone of polarizing activity. In the adult, expression is seen in brain, lung, liver, kidney and ocular tissues; lower levels in heart, skeletal muscle, and testis.

Inductioni

Activated by Sonic hedgehog.

Gene expression databases

BgeeiENSMUSG00000021466.
CleanExiMM_PTCH1.
ExpressionAtlasiQ61115. baseline and differential.
GenevisibleiQ61115. MM.

Interactioni

Subunit structurei

Interacts with SNX17 (By similarity). Interacts with IHH.By similarity1 Publication

GO - Molecular functioni

  • cyclin binding Source: BHF-UCL
  • hedgehog family protein binding Source: MGI
  • patched binding Source: Roslin
  • protein complex binding Source: BHF-UCL
  • smoothened binding Source: MGI

Protein-protein interaction databases

DIPiDIP-60268N.
STRINGi10090.ENSMUSP00000021921.

Structurei

3D structure databases

ProteinModelPortaliQ61115.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini424 – 584SSDPROSITE-ProRule annotationAdd BLAST161

Sequence similaritiesi

Belongs to the patched family.Curated
Contains 1 SSD (sterol-sensing) domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1935. Eukaryota.
ENOG410XRKU. LUCA.
GeneTreeiENSGT00800000124043.
HOGENOMiHOG000231331.
HOVERGENiHBG003801.
InParanoidiQ61115.
KOiK06225.
OMAiHVIKNDN.
OrthoDBiEOG091G0LGQ.
PhylomeDBiQ61115.
TreeFamiTF106489.

Family and domain databases

InterProiIPR003392. Ptc/Disp.
IPR000731. SSD.
IPR004766. TM_rcpt_patched.
[Graphical view]
PfamiPF02460. Patched. 1 hit.
PF12349. Sterol-sensing. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00918. 2A060602. 1 hit.
PROSITEiPS50156. SSD. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q61115-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASAGNAAGA LGRQAGGGRR RRTGGPHRAA PDRDYLHRPS YCDAAFALEQ
60 70 80 90 100
ISKGKATGRK APLWLRAKFQ RLLFKLGCYI QKNCGKFLVV GLLIFGAFAV
110 120 130 140 150
GLKAANLETN VEELWVEVGG RVSRELNYTR QKIGEEAMFN PQLMIQTPKE
160 170 180 190 200
EGANVLTTEA LLQHLDSALQ ASRVHVYMYN RQWKLEHLCY KSGELITETG
210 220 230 240 250
YMDQIIEYLY PCLIITPLDC FWEGAKLQSG TAYLLGKPPL RWTNFDPLEF
260 270 280 290 300
LEELKKINYQ VDSWEEMLNK AEVGHGYMDR PCLNPADPDC PATAPNKNST
310 320 330 340 350
KPLDVALVLN GGCQGLSRKY MHWQEELIVG GTVKNATGKL VSAHALQTMF
360 370 380 390 400
QLMTPKQMYE HFRGYDYVSH INWNEDRAAA ILEAWQRTYV EVVHQSVAPN
410 420 430 440 450
STQKVLPFTT TTLDDILKSF SDVSVIRVAS GYLLMLAYAC LTMLRWDCSK
460 470 480 490 500
SQGAVGLAGV LLVALSVAAG LGLCSLIGIS FNAATTQVLP FLALGVGVDD
510 520 530 540 550
VFLLAHAFSE TGQNKRIPFE DRTGECLKRT GASVALTSIS NVTAFFMAAL
560 570 580 590 600
IPIPALRAFS LQAAVVVVFN FAMVLLIFPA ILSMDLYRRE DRRLDIFCCF
610 620 630 640 650
TSPCVSRVIQ VEPQAYTEPH SNTRYSPPPP YTSHSFAHET HITMQSTVQL
660 670 680 690 700
RTEYDPHTHV YYTTAEPRSE ISVQPVTVTQ DNLSCQSPES TSSTRDLLSQ
710 720 730 740 750
FSDSSLHCLE PPCTKWTLSS FAEKHYAPFL LKPKAKVVVI LLFLGLLGVS
760 770 780 790 800
LYGTTRVRDG LDLTDIVPRE TREYDFIAAQ FKYFSFYNMY IVTQKADYPN
810 820 830 840 850
IQHLLYDLHK SFSNVKYVML EENKQLPQMW LHYFRDWLQG LQDAFDSDWE
860 870 880 890 900
TGRIMPNNYK NGSDDGVLAY KLLVQTGSRD KPIDISQLTK QRLVDADGII
910 920 930 940 950
NPSAFYIYLT AWVSNDPVAY AASQANIRPH RPEWVHDKAD YMPETRLRIP
960 970 980 990 1000
AAEPIEYAQF PFYLNGLRDT SDFVEAIEKV RVICNNYTSL GLSSYPNGYP
1010 1020 1030 1040 1050
FLFWEQYISL RHWLLLSISV VLACTFLVCA VFLLNPWTAG IIVMVLALMT
1060 1070 1080 1090 1100
VELFGMMGLI GIKLSAVPVV ILIASVGIGV EFTVHVALAF LTAIGDKNHR
1110 1120 1130 1140 1150
AMLALEHMFA PVLDGAVSTL LGVLMLAGSE FDFIVRYFFA VLAILTVLGV
1160 1170 1180 1190 1200
LNGLVLLPVL LSFFGPCPEV SPANGLNRLP TPSPEPPPSV VRFAVPPGHT
1210 1220 1230 1240 1250
NNGSDSSDSE YSSQTTVSGI SEELRQYEAQ QGAGGPAHQV IVEATENPVF
1260 1270 1280 1290 1300
ARSTVVHPDS RHQPPLTPRQ QPHLDSGSLS PGRQGQQPRR DPPREGLRPP
1310 1320 1330 1340 1350
PYRPRRDAFE ISTEGHSGPS NRDRSGPRGA RSHNPRNPTS TAMGSSVPSY
1360 1370 1380 1390 1400
CQPITTVTAS ASVTVAVHPP PGPGRNPRGG PCPGYESYPE TDHGVFEDPH
1410 1420 1430
VPFHVRCERR DSKVEVIELQ DVECEERPWG SSSN
Length:1,434
Mass (Da):159,273
Last modified:November 1, 1996 - v1
Checksum:iA5E9189E633173D0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U46155 mRNA. Translation: AAC98798.1.
CCDSiCCDS26592.1.
PIRiT30172.
RefSeqiNP_032983.1. NM_008957.3.
XP_006517222.1. XM_006517159.3.
UniGeneiMm.228798.
Mm.488634.

Genome annotation databases

EnsembliENSMUST00000021921; ENSMUSP00000021921; ENSMUSG00000021466.
GeneIDi19206.
KEGGimmu:19206.
UCSCiuc007qxv.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U46155 mRNA. Translation: AAC98798.1.
CCDSiCCDS26592.1.
PIRiT30172.
RefSeqiNP_032983.1. NM_008957.3.
XP_006517222.1. XM_006517159.3.
UniGeneiMm.228798.
Mm.488634.

3D structure databases

ProteinModelPortaliQ61115.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-60268N.
STRINGi10090.ENSMUSP00000021921.

PTM databases

iPTMnetiQ61115.
PhosphoSitePlusiQ61115.

Proteomic databases

PaxDbiQ61115.
PRIDEiQ61115.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000021921; ENSMUSP00000021921; ENSMUSG00000021466.
GeneIDi19206.
KEGGimmu:19206.
UCSCiuc007qxv.1. mouse.

Organism-specific databases

CTDi5727.
MGIiMGI:105373. Ptch1.

Phylogenomic databases

eggNOGiKOG1935. Eukaryota.
ENOG410XRKU. LUCA.
GeneTreeiENSGT00800000124043.
HOGENOMiHOG000231331.
HOVERGENiHBG003801.
InParanoidiQ61115.
KOiK06225.
OMAiHVIKNDN.
OrthoDBiEOG091G0LGQ.
PhylomeDBiQ61115.
TreeFamiTF106489.

Enzyme and pathway databases

ReactomeiR-MMU-5610787. Hedgehog 'off' state.
R-MMU-5632681. Ligand-receptor interactions.
R-MMU-5632684. Hedgehog 'on' state.
R-MMU-5635838. Activation of SMO.

Miscellaneous databases

ChiTaRSiPtch1. mouse.
PROiQ61115.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000021466.
CleanExiMM_PTCH1.
ExpressionAtlasiQ61115. baseline and differential.
GenevisibleiQ61115. MM.

Family and domain databases

InterProiIPR003392. Ptc/Disp.
IPR000731. SSD.
IPR004766. TM_rcpt_patched.
[Graphical view]
PfamiPF02460. Patched. 1 hit.
PF12349. Sterol-sensing. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00918. 2A060602. 1 hit.
PROSITEiPS50156. SSD. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPTC1_MOUSE
AccessioniPrimary (citable) accession number: Q61115
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 1, 1996
Last modified: November 30, 2016
This is version 138 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.