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Protein

45 kDa calcium-binding protein

Gene

Sdf4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May regulate calcium-dependent activities in the endoplasmic reticulum lumen or post-ER compartment.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Calcium bindingi110 – 121121PROSITE-ProRule annotationAdd
BLAST
Calcium bindingi149 – 160122PROSITE-ProRule annotationAdd
BLAST
Calcium bindingi208 – 219123PROSITE-ProRule annotationAdd
BLAST
Calcium bindingi245 – 256124PROSITE-ProRule annotationAdd
BLAST
Calcium bindingi290 – 301125PROSITE-ProRule annotationAdd
BLAST
Calcium bindingi326 – 337126PROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  • calcium ion binding Source: BHF-UCL

GO - Biological processi

  • cerebellum development Source: BHF-UCL
  • fat cell differentiation Source: BHF-UCL
  • response to ethanol Source: BHF-UCL
  • UV protection Source: BHF-UCL
Complete GO annotation...

Keywords - Ligandi

Calcium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
45 kDa calcium-binding protein
Short name:
Cab45
Alternative name(s):
Stromal cell-derived factor 4
Short name:
SDF-4
Gene namesi
Name:Sdf4
Synonyms:Cab45
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:108079. Sdf4.

Subcellular locationi

GO - Cellular componenti

  • extracellular exosome Source: MGI
  • Golgi apparatus Source: MGI
  • Golgi lumen Source: BHF-UCL
  • late endosome Source: BHF-UCL
  • membrane Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3535Sequence analysisAdd
BLAST
Chaini36 – 36132645 kDa calcium-binding proteinPRO_0000004157Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi39 – 391N-linked (GlcNAc...)Sequence analysis
Modified residuei192 – 1921PhosphothreonineBy similarity
Modified residuei216 – 2161PhosphothreonineBy similarity
Modified residuei264 – 2641PhosphothreonineBy similarity
Modified residuei298 – 2981PhosphothreonineBy similarity

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

EPDiQ61112.
MaxQBiQ61112.
PaxDbiQ61112.
PRIDEiQ61112.

PTM databases

PhosphoSiteiQ61112.

Expressioni

Tissue specificityi

Ubiquitous.1 Publication

Gene expression databases

BgeeiQ61112.
CleanExiMM_SDF4.
GenevisibleiQ61112. MM.

Interactioni

Protein-protein interaction databases

BioGridi203141. 1 interaction.
STRINGi10090.ENSMUSP00000053175.

Structurei

3D structure databases

ProteinModelPortaliQ61112.
SMRiQ61112. Positions 98-165, 203-341.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini97 – 13236EF-hand 1PROSITE-ProRule annotationAdd
BLAST
Domaini136 – 17136EF-hand 2PROSITE-ProRule annotationAdd
BLAST
Domaini196 – 23136EF-hand 3PROSITE-ProRule annotationAdd
BLAST
Domaini232 – 26736EF-hand 4PROSITE-ProRule annotationAdd
BLAST
Domaini277 – 31236EF-hand 5PROSITE-ProRule annotationAdd
BLAST
Domaini313 – 34836EF-hand 6PROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni308 – 36154Necessary for intracellular retention in Golgi apparatus lumenAdd
BLAST

Domaini

Binds calcium, probably via its EF-hands.

Sequence similaritiesi

Belongs to the CREC family.Curated
Contains 6 EF-hand domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiKOG4251. Eukaryota.
ENOG410XSH7. LUCA.
GeneTreeiENSGT00550000074546.
HOGENOMiHOG000007329.
HOVERGENiHBG097344.
InParanoidiQ61112.
KOiK19934.
OMAiNKMHFRA.
OrthoDBiEOG7M0NSC.
PhylomeDBiQ61112.
TreeFamiTF314849.

Family and domain databases

Gene3Di1.10.238.10. 3 hits.
InterProiIPR027240. CAB45.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
[Graphical view]
PANTHERiPTHR10827:SF51. PTHR10827:SF51. 1 hit.
PfamiPF13202. EF-hand_5. 1 hit.
PF13499. EF-hand_7. 1 hit.
[Graphical view]
SMARTiSM00054. EFh. 5 hits.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 2 hits.
PROSITEiPS00018. EF_HAND_1. 5 hits.
PS50222. EF_HAND_2. 5 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q61112-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MVWLVAMTPR QSSLCGLAAH GLWFLGLVLL MDATARPANH SSTRERAANR
60 70 80 90 100
EENEIMPPDH LNGVKLEMDG HLNKDFHQEV FLGKDMDGFD EDSEPRRSRR
110 120 130 140 150
KLMVIFSKVD VNTDRRISAK EMQHWIMEKT AEHFQEAVKE NKLHFRAVDP
160 170 180 190 200
DGDGHVSWDE YKVKFLASKG HNEREIAEAI KNHEELKVDE ETQEVLGNLR
210 220 230 240 250
DRWYQADNPP ADLLLTEDEF LSFLHPEHSR GMLKFMVKEI FRDLDQDGDK
260 270 280 290 300
QLSLPEFISL PVGTVENQQG QDIDDNWVKD RKKEFEELID SNHDGIVTME
310 320 330 340 350
ELENYMDPMN EYNALNEAKQ MIAIADENQN HHLEPEEILK YSEFFTGSKL
360
MDYARNVHEE F
Length:361
Mass (Da):42,064
Last modified:November 1, 1997 - v1
Checksum:i2599E99F5F81A913
GO
Isoform 2 (identifier: Q61112-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     192-218: TQEVLGNLRDRWYQADNPPADLLLTED → IALHFLLLTGGPLSCSSGETSICLSRL
     219-361: Missing.

Show »
Length:218
Mass (Da):24,843
Checksum:i4E2CEDDA099881ED
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti281 – 2811R → K in CAB45A. 1 Publication

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei192 – 21827TQEVL…LLTED → IALHFLLLTGGPLSCSSGET SICLSRL in isoform 2. 1 PublicationVSP_037450Add
BLAST
Alternative sequencei219 – 361143Missing in isoform 2. 1 PublicationVSP_037451Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U45977 mRNA. Translation: AAB01812.1.
U45978 mRNA. Translation: AAB01813.1.
D50461 mRNA. Translation: BAA09052.1.
AK157860 mRNA. Translation: BAE34238.1.
AK163400 mRNA. Translation: BAE37334.1.
AK167113 mRNA. Translation: BAE39261.1.
AL627204 Genomic DNA. Translation: CAM13857.1.
AL627204 Genomic DNA. Translation: CAO78017.1.
CH466594 Genomic DNA. Translation: EDL15066.1.
BC068152 mRNA. Translation: AAH68152.1.
CCDSiCCDS19054.1. [Q61112-1]
CCDS80198.1. [Q61112-2]
RefSeqiNP_001289396.1. NM_001302467.1. [Q61112-1]
NP_001289397.1. NM_001302468.1. [Q61112-1]
NP_001289398.1. NM_001302469.1. [Q61112-2]
NP_035471.1. NM_011341.5. [Q61112-1]
UniGeneiMm.293517.

Genome annotation databases

EnsembliENSMUST00000050078; ENSMUSP00000053175; ENSMUSG00000029076. [Q61112-1]
ENSMUST00000097734; ENSMUSP00000095340; ENSMUSG00000029076. [Q61112-2]
ENSMUST00000105578; ENSMUSP00000101203; ENSMUSG00000029076. [Q61112-1]
ENSMUST00000105579; ENSMUSP00000101204; ENSMUSG00000029076. [Q61112-1]
GeneIDi20318.
KEGGimmu:20318.
UCSCiuc008wfr.2. mouse. [Q61112-1]
uc056zys.1. mouse. [Q61112-2]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U45977 mRNA. Translation: AAB01812.1.
U45978 mRNA. Translation: AAB01813.1.
D50461 mRNA. Translation: BAA09052.1.
AK157860 mRNA. Translation: BAE34238.1.
AK163400 mRNA. Translation: BAE37334.1.
AK167113 mRNA. Translation: BAE39261.1.
AL627204 Genomic DNA. Translation: CAM13857.1.
AL627204 Genomic DNA. Translation: CAO78017.1.
CH466594 Genomic DNA. Translation: EDL15066.1.
BC068152 mRNA. Translation: AAH68152.1.
CCDSiCCDS19054.1. [Q61112-1]
CCDS80198.1. [Q61112-2]
RefSeqiNP_001289396.1. NM_001302467.1. [Q61112-1]
NP_001289397.1. NM_001302468.1. [Q61112-1]
NP_001289398.1. NM_001302469.1. [Q61112-2]
NP_035471.1. NM_011341.5. [Q61112-1]
UniGeneiMm.293517.

3D structure databases

ProteinModelPortaliQ61112.
SMRiQ61112. Positions 98-165, 203-341.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi203141. 1 interaction.
STRINGi10090.ENSMUSP00000053175.

PTM databases

PhosphoSiteiQ61112.

Proteomic databases

EPDiQ61112.
MaxQBiQ61112.
PaxDbiQ61112.
PRIDEiQ61112.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000050078; ENSMUSP00000053175; ENSMUSG00000029076. [Q61112-1]
ENSMUST00000097734; ENSMUSP00000095340; ENSMUSG00000029076. [Q61112-2]
ENSMUST00000105578; ENSMUSP00000101203; ENSMUSG00000029076. [Q61112-1]
ENSMUST00000105579; ENSMUSP00000101204; ENSMUSG00000029076. [Q61112-1]
GeneIDi20318.
KEGGimmu:20318.
UCSCiuc008wfr.2. mouse. [Q61112-1]
uc056zys.1. mouse. [Q61112-2]

Organism-specific databases

CTDi51150.
MGIiMGI:108079. Sdf4.

Phylogenomic databases

eggNOGiKOG4251. Eukaryota.
ENOG410XSH7. LUCA.
GeneTreeiENSGT00550000074546.
HOGENOMiHOG000007329.
HOVERGENiHBG097344.
InParanoidiQ61112.
KOiK19934.
OMAiNKMHFRA.
OrthoDBiEOG7M0NSC.
PhylomeDBiQ61112.
TreeFamiTF314849.

Miscellaneous databases

ChiTaRSiSdf4. mouse.
NextBioi298107.
PROiQ61112.
SOURCEiSearch...

Gene expression databases

BgeeiQ61112.
CleanExiMM_SDF4.
GenevisibleiQ61112. MM.

Family and domain databases

Gene3Di1.10.238.10. 3 hits.
InterProiIPR027240. CAB45.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
[Graphical view]
PANTHERiPTHR10827:SF51. PTHR10827:SF51. 1 hit.
PfamiPF13202. EF-hand_5. 1 hit.
PF13499. EF-hand_7. 1 hit.
[Graphical view]
SMARTiSM00054. EFh. 5 hits.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 2 hits.
PROSITEiPS00018. EF_HAND_1. 5 hits.
PS50222. EF_HAND_2. 5 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cab45, a novel (Ca2+)-binding protein localized to the Golgi lumen."
    Scherer P.E., Lederkremer G.Z., Williams S., Fogliano M., Baldini G., Lodish H.F.
    J. Cell Biol. 133:257-268(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY, VARIANT LYS-281.
  2. "Characterization of novel secreted and membrane proteins isolated by the signal sequence trap method."
    Shirozu M., Tada H., Tashiro K., Nakamura T., Lopez N.D., Nazarea M., Hamada T., Sato T., Nakano T., Honjo T.
    Genomics 37:273-280(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
  3. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Strain: C57BL/6J.
    Tissue: Bone marrow and Egg.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  5. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: C57BL/6J.
    Tissue: Brain.
  7. "Lumenal protein sorting to the constitutive secretory pathway of a regulated secretory cell."
    Lara-Lemus R., Liu M., Turner M.D., Scherer P., Stenbeck G., Iyengar P., Arvan P.
    J. Cell Sci. 119:1833-1842(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
  8. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Kidney, Liver, Pancreas and Testis.

Entry informationi

Entry nameiCAB45_MOUSE
AccessioniPrimary (citable) accession number: Q61112
Secondary accession number(s): Q3TK84, Q3TQP8, Q61113
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: May 11, 2016
This is version 123 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.