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Protein

Zinc finger protein ubi-d4

Gene

Dpf2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May be a transcription factor required for the apoptosis response following survival factor withdrawal from myeloid cells. Might also have a role in the development and maturation of lymphoid cells.1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri209 – 23224C2H2-typePROSITE-ProRule annotationAdd
BLAST
Zinc fingeri270 – 33061PHD-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri327 – 37751PHD-type 2PROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Apoptosis, Transcription, Transcription regulation

Keywords - Ligandi

Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Zinc finger protein ubi-d4
Alternative name(s):
Apoptosis response zinc finger protein
BRG1-associated factor 45D
Short name:
BAF45D
D4, zinc and double PHD fingers family 2
Protein requiem
Gene namesi
Name:Dpf2
Synonyms:Baf45d, Req, Ubid4
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 19

Organism-specific databases

MGIiMGI:109529. Dpf2.

Subcellular locationi

GO - Cellular componenti

  • BAF-type complex Source: MGI
  • centrosome Source: MGI
  • cytoplasm Source: MGI
  • intracellular membrane-bounded organelle Source: MGI
  • nuclear chromatin Source: MGI
  • nucleoplasm Source: MGI
  • nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 391390Zinc finger protein ubi-d4PRO_0000168150Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineBy similarity
Cross-linki10 – 10Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki99 – 99Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki107 – 107Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei142 – 1421PhosphoserineCombined sources
Modified residuei172 – 1721PhosphotyrosineBy similarity
Modified residuei176 – 1761PhosphothreonineCombined sources
Modified residuei200 – 2001PhosphoserineBy similarity
Modified residuei244 – 2441PhosphoserineBy similarity
Modified residuei280 – 2801PhosphoserineBy similarity

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ61103.
MaxQBiQ61103.
PaxDbiQ61103.
PeptideAtlasiQ61103.
PRIDEiQ61103.

PTM databases

iPTMnetiQ61103.
PhosphoSiteiQ61103.

Expressioni

Tissue specificityi

In embryo, highest levels are seen in brain, eyes, thymus and olfactory epithelium in nose, whereas several other tissues, including the musculoskeletal system, show moderate expression. In adult, higher expression in testis, medium in thymus and spleen, lower in certain parts of the brain as the hippocampus. No expression in adult heart, lung, liver, duodenum and kidney.1 Publication

Developmental stagei

Already detected at embryonic day 8.5. Expressed ubiquitously throughout the developing spinal cord, brain and other embryonic tissues at E10.5-E16.5.1 Publication

Gene expression databases

BgeeiQ61103.
CleanExiMM_DPF2.
ExpressionAtlasiQ61103. baseline and differential.
GenevisibleiQ61103. MM.

Interactioni

Protein-protein interaction databases

BioGridi202862. 10 interactions.
DIPiDIP-59247N.
IntActiQ61103. 5 interactions.
MINTiMINT-4132218.
STRINGi10090.ENSMUSP00000120125.

Structurei

3D structure databases

ProteinModelPortaliQ61103.
SMRiQ61103. Positions 203-234, 271-383.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the requiem/DPF family.Curated
Contains 1 C2H2-type zinc finger.PROSITE-ProRule annotation
Contains 2 PHD-type zinc fingers.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri209 – 23224C2H2-typePROSITE-ProRule annotationAdd
BLAST
Zinc fingeri270 – 33061PHD-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri327 – 37751PHD-type 2PROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG1244. Eukaryota.
ENOG410YDAG. LUCA.
GeneTreeiENSGT00530000063194.
HOVERGENiHBG004475.
InParanoidiQ61103.
KOiK13196.
OrthoDBiEOG7JHM5F.
PhylomeDBiQ61103.
TreeFamiTF318971.

Family and domain databases

Gene3Di3.30.40.10. 2 hits.
InterProiIPR025750. Requiem/DPF_N_dom.
IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR011011. Znf_FYVE_PHD.
IPR001965. Znf_PHD.
IPR019787. Znf_PHD-finger.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PfamiPF00628. PHD. 1 hit.
PF14051. Requiem_N. 1 hit.
[Graphical view]
SMARTiSM00249. PHD. 2 hits.
SM00355. ZnF_C2H2. 1 hit.
[Graphical view]
SUPFAMiSSF57903. SSF57903. 2 hits.
PROSITEiPS01359. ZF_PHD_1. 1 hit.
PS50016. ZF_PHD_2. 2 hits.
PS00028. ZINC_FINGER_C2H2_1. 1 hit.
PS50157. ZINC_FINGER_C2H2_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q61103-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAVVENVVK LLGEQYYKDA MEQCHNYNAR LCAERSVRLP FLDSQTGVAQ
60 70 80 90 100
SNCYIWMEKR HRGPGLASGQ LYSYPARRWR KKRRAHPPED PRLSFPSIKP
110 120 130 140 150
DTDQTLKKEG LISQDGSSLE ALLRTDPLEK RGAPDPRVDD DSLGEFPVSN
160 170 180 190 200
SRARKRIIEP DDFLDDLDDE DYEEDTPKRR GKGKSKSKGV SSARKKLDAS
210 220 230 240 250
ILEDRDKPYA CDICGKRYKN RPGLSYHYAH SHLAEEEGED KEDSRPPTPV
260 270 280 290 300
SQRSEEQKSK KGPDGLALPN NYCDFCLGDS KINKKTGQPE ELVSCSDCGR
310 320 330 340 350
SGHPSCLQFT PVMMAAVKTY RWQCIECKCC NLCGTSENDD QLLFCDDCDR
360 370 380 390
GYHMYCLTPS MSEPPEGSWS CHLCLDLLKE KASIYQNQNS S
Length:391
Mass (Da):44,230
Last modified:November 1, 1996 - v1
Checksum:i25598DBC80915BF2
GO

Sequence cautioni

The sequence AAA64637.1 differs from that shown. Reason: Erroneous initiation. Curated
The sequence AAH07188.1 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti176 – 1761T → R in AAA64637 (PubMed:7961935).Curated
Sequence conflicti187 – 1882Missing in AAH07188 (PubMed:15489334).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U43921 mRNA. Translation: AAC52783.1.
AF108134 Genomic DNA. Translation: AAF21306.1.
AK004812 mRNA. Translation: BAB23583.1.
AK138047 mRNA. Translation: BAE23543.1.
AK144106 mRNA. Translation: BAE25702.1.
AK144848 mRNA. Translation: BAE26098.1.
AK144954 mRNA. Translation: BAE26153.1.
BC007188 mRNA. Translation: AAH07188.1. Different initiation.
BC012709 mRNA. Translation: AAH12709.1.
U10435 mRNA. Translation: AAA64637.1. Different initiation.
CCDSiCCDS37892.1.
RefSeqiNP_001278007.1. NM_001291078.1.
NP_035392.1. NM_011262.5.
UniGeneiMm.2651.

Genome annotation databases

EnsembliENSMUST00000136983; ENSMUSP00000120125; ENSMUSG00000024826.
GeneIDi19708.
KEGGimmu:19708.
UCSCiuc008gfw.3. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U43921 mRNA. Translation: AAC52783.1.
AF108134 Genomic DNA. Translation: AAF21306.1.
AK004812 mRNA. Translation: BAB23583.1.
AK138047 mRNA. Translation: BAE23543.1.
AK144106 mRNA. Translation: BAE25702.1.
AK144848 mRNA. Translation: BAE26098.1.
AK144954 mRNA. Translation: BAE26153.1.
BC007188 mRNA. Translation: AAH07188.1. Different initiation.
BC012709 mRNA. Translation: AAH12709.1.
U10435 mRNA. Translation: AAA64637.1. Different initiation.
CCDSiCCDS37892.1.
RefSeqiNP_001278007.1. NM_001291078.1.
NP_035392.1. NM_011262.5.
UniGeneiMm.2651.

3D structure databases

ProteinModelPortaliQ61103.
SMRiQ61103. Positions 203-234, 271-383.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi202862. 10 interactions.
DIPiDIP-59247N.
IntActiQ61103. 5 interactions.
MINTiMINT-4132218.
STRINGi10090.ENSMUSP00000120125.

PTM databases

iPTMnetiQ61103.
PhosphoSiteiQ61103.

Proteomic databases

EPDiQ61103.
MaxQBiQ61103.
PaxDbiQ61103.
PeptideAtlasiQ61103.
PRIDEiQ61103.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000136983; ENSMUSP00000120125; ENSMUSG00000024826.
GeneIDi19708.
KEGGimmu:19708.
UCSCiuc008gfw.3. mouse.

Organism-specific databases

CTDi5977.
MGIiMGI:109529. Dpf2.

Phylogenomic databases

eggNOGiKOG1244. Eukaryota.
ENOG410YDAG. LUCA.
GeneTreeiENSGT00530000063194.
HOVERGENiHBG004475.
InParanoidiQ61103.
KOiK13196.
OrthoDBiEOG7JHM5F.
PhylomeDBiQ61103.
TreeFamiTF318971.

Miscellaneous databases

ChiTaRSiDpf2. mouse.
PROiQ61103.
SOURCEiSearch...

Gene expression databases

BgeeiQ61103.
CleanExiMM_DPF2.
ExpressionAtlasiQ61103. baseline and differential.
GenevisibleiQ61103. MM.

Family and domain databases

Gene3Di3.30.40.10. 2 hits.
InterProiIPR025750. Requiem/DPF_N_dom.
IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR011011. Znf_FYVE_PHD.
IPR001965. Znf_PHD.
IPR019787. Znf_PHD-finger.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PfamiPF00628. PHD. 1 hit.
PF14051. Requiem_N. 1 hit.
[Graphical view]
SMARTiSM00249. PHD. 2 hits.
SM00355. ZnF_C2H2. 1 hit.
[Graphical view]
SUPFAMiSSF57903. SSF57903. 2 hits.
PROSITEiPS01359. ZF_PHD_1. 1 hit.
PS50016. ZF_PHD_2. 2 hits.
PS00028. ZINC_FINGER_C2H2_1. 1 hit.
PS50157. ZINC_FINGER_C2H2_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Structure and expression of two members of the d4 gene family in mouse."
    Mertsalov I.B., Kulikova D.A., Alimova-Kost M.V., Ninkina N.N., Korochkin L.I., Buchman V.L.
    Mamm. Genome 11:72-74(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
    Strain: CD-1.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Lung, Mammary gland, Pancreas and Thymus.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Colon and Mammary tumor.
  4. "Requiem: a novel zinc finger gene essential for apoptosis in myeloid cells."
    Gabig T.G., Mantel P.L., Rosli R., Crean C.D.
    J. Biol. Chem. 269:29515-29519(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 6-391, FUNCTION.
    Strain: C57BL/6J.
    Tissue: Spleen.
  5. Cited for: TISSUE SPECIFICITY.
  6. "An essential switch in subunit composition of a chromatin remodeling complex during neural development."
    Lessard J., Wu J.I., Ranish J.A., Wan M., Winslow M.M., Staahl B.T., Wu H., Aebersold R., Graef I.A., Crabtree G.R.
    Neuron 55:201-215(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY, DEVELOPMENTAL STAGE.
  7. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-142, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.
  8. "Large scale localization of protein phosphorylation by use of electron capture dissociation mass spectrometry."
    Sweet S.M., Bailey C.M., Cunningham D.L., Heath J.K., Cooper H.J.
    Mol. Cell. Proteomics 8:904-912(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Embryonic fibroblast.
  9. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-142 AND THR-176, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Brown adipose tissue, Kidney, Lung, Pancreas, Spleen and Testis.

Entry informationi

Entry nameiREQU_MOUSE
AccessioniPrimary (citable) accession number: Q61103
Secondary accession number(s): Q3UNP5, Q60663, Q9QYA3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: July 6, 2016
This is version 143 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.