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Protein

Myeloblastin

Gene

Prtn3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Polymorphonuclear leukocyte serine protease that degrades elastin, fibronectin, laminin, vitronectin, and collagen types I, III, and IV (in vitro) and causes emphysema when administered by tracheal insufflation to hamsters.By similarity

Catalytic activityi

Hydrolysis of proteins, including elastin, by preferential cleavage: -Ala-|-Xaa- > -Val-|-Xaa-.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei73 – 731Charge relay systemBy similarity
Active sitei120 – 1201Charge relay systemBy similarity
Active sitei205 – 2051Charge relay systemBy similarity

GO - Molecular functioni

  1. enzyme binding Source: MGI
  2. serine-type endopeptidase activity Source: InterPro

GO - Biological processi

  1. collagen catabolic process Source: UniProtKB-KW
  2. mature conventional dendritic cell differentiation Source: MGI
  3. negative regulation of phagocytosis Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Keywords - Biological processi

Collagen degradation

Enzyme and pathway databases

BRENDAi3.4.21.76. 3474.

Protein family/group databases

MEROPSiS01.134.

Names & Taxonomyi

Protein namesi
Recommended name:
Myeloblastin (EC:3.4.21.76)
Alternative name(s):
Proteinase 3
Short name:
PR-3
Gene namesi
Name:Prtn3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 10

Organism-specific databases

MGIiMGI:893580. Prtn3.

Subcellular locationi

GO - Cellular componenti

  1. cytosol Source: MGI
  2. extracellular matrix Source: Ensembl
  3. extracellular space Source: MGI
  4. extracellular vesicular exosome Source: MGI
  5. plasma membrane Source: MGI
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2727By similarityAdd
BLAST
Propeptidei28 – 292By similarityPRO_0000027710
Chaini30 – 250221MyeloblastinPRO_0000027711Add
BLAST
Propeptidei251 – 2544By similarityPRO_0000027712

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi58 ↔ 74PROSITE-ProRule annotation
Glycosylationi127 – 1271N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi154 ↔ 211PROSITE-ProRule annotation
Glycosylationi176 – 1761N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi184 ↔ 190PROSITE-ProRule annotation
Disulfide bondi201 ↔ 226PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

PaxDbiQ61096.
PRIDEiQ61096.

Expressioni

Gene expression databases

BgeeiQ61096.
CleanExiMM_PRTN3.
ExpressionAtlasiQ61096. baseline and differential.
GenevestigatoriQ61096.

Interactioni

Protein-protein interaction databases

IntActiQ61096. 1 interaction.
MINTiMINT-4108835.

Structurei

3D structure databases

ProteinModelPortaliQ61096.
SMRiQ61096. Positions 30-250.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini30 – 250221Peptidase S1PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase S1 family. Elastase subfamily.PROSITE-ProRule annotation
Contains 1 peptidase S1 domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG5640.
GeneTreeiENSGT00760000118895.
HOGENOMiHOG000251820.
HOVERGENiHBG013304.
InParanoidiQ61096.
KOiK01350.
OrthoDBiEOG7MKW6Q.
PhylomeDBiQ61096.
TreeFamiTF335284.

Family and domain databases

InterProiIPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q61096-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSGSYPSPKG IHPFLLLALV VGGAVQASKI VGGHEARPHS RPYVASLQLS
60 70 80 90 100
RFPGSHFCGG TLIHPRFVLT AAHCLQDISW QLVTVVLGAH DLLSSEPEQQ
110 120 130 140 150
KFTISQVFQN NYNPEENLND VLLLQLNRTA SLGKEVAVAS LPQQDQTLSQ
160 170 180 190 200
GTQCLAMGWG RLGTQAPTPR VLQELNVTVV TFLCREHNVC TLVPRRAAGI
210 220 230 240 250
CFGDSGGPLI CNGILHGVDS FVIRECASLQ FPDFFARVSM YVDWIQNVLR

GAEP
Length:254
Mass (Da):27,626
Last modified:August 1, 1999 - v2
Checksum:i00CEB989A3CB79CA
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti2 – 21S → A in AAB58055. (PubMed:9187364)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF082186 Genomic DNA. Translation: AAC79701.1.
U97073 mRNA. Translation: AAB58055.1.
U43525 mRNA. Translation: AAB67271.1.
CCDSiCCDS23993.1.
RefSeqiNP_035308.2. NM_011178.2.
UniGeneiMm.2364.

Genome annotation databases

EnsembliENSMUST00000006679; ENSMUSP00000006679; ENSMUSG00000057729.
GeneIDi19152.
KEGGimmu:19152.
UCSCiuc007gah.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF082186 Genomic DNA. Translation: AAC79701.1.
U97073 mRNA. Translation: AAB58055.1.
U43525 mRNA. Translation: AAB67271.1.
CCDSiCCDS23993.1.
RefSeqiNP_035308.2. NM_011178.2.
UniGeneiMm.2364.

3D structure databases

ProteinModelPortaliQ61096.
SMRiQ61096. Positions 30-250.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ61096. 1 interaction.
MINTiMINT-4108835.

Chemistry

BindingDBiQ61096.
ChEMBLiCHEMBL5384.

Protein family/group databases

MEROPSiS01.134.

Proteomic databases

PaxDbiQ61096.
PRIDEiQ61096.

Protocols and materials databases

DNASUi19152.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000006679; ENSMUSP00000006679; ENSMUSG00000057729.
GeneIDi19152.
KEGGimmu:19152.
UCSCiuc007gah.1. mouse.

Organism-specific databases

CTDi5657.
MGIiMGI:893580. Prtn3.

Phylogenomic databases

eggNOGiCOG5640.
GeneTreeiENSGT00760000118895.
HOGENOMiHOG000251820.
HOVERGENiHBG013304.
InParanoidiQ61096.
KOiK01350.
OrthoDBiEOG7MKW6Q.
PhylomeDBiQ61096.
TreeFamiTF335284.

Enzyme and pathway databases

BRENDAi3.4.21.76. 3474.

Miscellaneous databases

ChiTaRSiPrtn3. mouse.
NextBioi295792.
PROiQ61096.
SOURCEiSearch...

Gene expression databases

BgeeiQ61096.
CleanExiMM_PRTN3.
ExpressionAtlasiQ61096. baseline and differential.
GenevestigatoriQ61096.

Family and domain databases

InterProiIPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Characterization and localization of the genes for mouse proteinase-3 (Prtn3) and neutrophil elastase (Ela2)."
    Sturrock A., Franklin K.F., Wu S.-Q., Hoidal J.R.
    Cytogenet. Cell Genet. 83:104-108(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 129/SvJ.
  2. "Cloning and functional expression of the murine homologue of proteinase 3: implications for the design of murine models of vasculitis."
    Jenne D.E., Froehlich L., Hummel A.M., Specks U.
    FEBS Lett. 408:187-190(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: 129/Sv.
  3. "Characterization of cDNA clones encoding mouse proteinase 3 (myeloblastine) and cathepsin G."
    Aveskogh M., Lutzelschwab C., Huang M.R., Hellman L.
    Immunogenetics 46:181-191(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 2-254.
    Strain: BALB/c.

Entry informationi

Entry nameiPRTN3_MOUSE
AccessioniPrimary (citable) accession number: Q61096
Secondary accession number(s): O08809
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 30, 2002
Last sequence update: August 1, 1999
Last modified: February 4, 2015
This is version 110 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.