Q61096 (PRTN3_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Myeloblastin
EC=3.4.21.76

Alternative name(s):
Proteinase 3
Short name=PR-3

Gene names

Name:

Prtn3

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	254 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Polymorphonuclear leukocyte serine protease that degrades elastin, fibronectin, laminin, vitronectin, and collagen types I, III, and IV (in vitro) and causes emphysema when administered by tracheal insufflation to hamsters By similarity.
Catalytic activity	Hydrolysis of proteins, including elastin, by preferential cleavage: -Ala-|-Xaa- > -Val-|-Xaa-.
Sequence similarities	Belongs to the peptidase S1 family. Elastase subfamily. Contains 1 peptidase S1 domain.

Ontologies

Keywords
Biological process	Collagen degradation
Domain	Signal
Molecular function	Hydrolase Protease Serine protease
PTM	Disulfide bond Glycoprotein Zymogen
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	collagen catabolic process Inferred from electronic annotation. Source: UniProtKB-KW mature dendritic cell differentiation Inferred from electronic annotation. Source: Compara negative regulation of phagocytosis Inferred from electronic annotation. Source: Compara proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytosol Inferred from electronic annotation. Source: Compara extracellular matrix Inferred from electronic annotation. Source: Compara plasma membrane Inferred from electronic annotation. Source: Compara
Molecular_function	serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 27	27	By similarity
	Propeptide	28 – 29	2	By similarity		PRO_0000027710
	Chain	30 – 250	221	Myeloblastin		PRO_0000027711
	Propeptide	251 – 254	4	By similarity		PRO_0000027712
Regions
	Domain	30 – 250	221	Peptidase S1
Sites
	Active site	73	1	Charge relay system By similarity
	Active site	120	1	Charge relay system By similarity
	Active site	205	1	Charge relay system By similarity
Amino acid modifications
	Glycosylation	127	1	N-linked (GlcNAc...) Potential
	Glycosylation	176	1	N-linked (GlcNAc...) Potential
	Disulfide bond	58 ↔ 74		By similarity
	Disulfide bond	154 ↔ 211		By similarity
	Disulfide bond	184 ↔ 190		By similarity
	Disulfide bond	201 ↔ 226		By similarity
Experimental info
	Sequence conflict	2	1	S → A in AAB58055. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q61096 [UniParc]. Last modified August 1, 1999. Version 2. Checksum: 00CEB989A3CB79CA	FASTA	254	27,626
	10 20 30 40 50 60 MSGSYPSPKG IHPFLLLALV VGGAVQASKI VGGHEARPHS RPYVASLQLS RFPGSHFCGG 70 80 90 100 110 120 TLIHPRFVLT AAHCLQDISW QLVTVVLGAH DLLSSEPEQQ KFTISQVFQN NYNPEENLND 130 140 150 160 170 180 VLLLQLNRTA SLGKEVAVAS LPQQDQTLSQ GTQCLAMGWG RLGTQAPTPR VLQELNVTVV 190 200 210 220 230 240 TFLCREHNVC TLVPRRAAGI CFGDSGGPLI CNGILHGVDS FVIRECASLQ FPDFFARVSM 250 YVDWIQNVLR GAEP

References

[1]	"Characterization and localization of the genes for mouse proteinase-3 (Prtn3) and neutrophil elastase (Ela2)." Sturrock A., Franklin K.F., Wu S.-Q., Hoidal J.R. Cytogenet. Cell Genet. 83:104-108(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 129/SvJ.
[2]	"Cloning and functional expression of the murine homologue of proteinase 3: implications for the design of murine models of vasculitis." Jenne D.E., Froehlich L., Hummel A.M., Specks U. FEBS Lett. 408:187-190(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: 129/Sv.
[3]	"Characterization of cDNA clones encoding mouse proteinase 3 (myeloblastine) and cathepsin G." Aveskogh M., Lutzelschwab C., Huang M.R., Hellman L. Immunogenetics 46:181-191(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 2-254. Strain: BALB/c.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF082186 Genomic DNA. Translation: AAC79701.1. U97073 mRNA. Translation: AAB58055.1. U43525 mRNA. Translation: AAB67271.1.
IPI	IPI00117121.
RefSeq	NP_035308.2. NM_011178.2.
UniGene	Mm.2364.
3D structure databases
ProteinModelPortal	Q61096.
SMR	Q61096. Positions 30-250.
ModBase	Search...
Protein family/group databases
MEROPS	S01.134.
Proteomic databases
PaxDb	Q61096.
PRIDE	Q61096.
Protocols and materials databases
DNASU	19152.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000006679; ENSMUSP00000006679; ENSMUSG00000057729.
GeneID	19152.
KEGG	mmu:19152.
UCSC	uc007gah.1. mouse.
Organism-specific databases
CTD	5657.
MGI	MGI:893580. Prtn3.
Phylogenomic databases
eggNOG	COG5640.
GeneTree	ENSGT00700000104045.
HOGENOM	HOG000251820.
HOVERGEN	HBG013304.
InParanoid	Q61096.
KO	K01350.
OrthoDB	EOG4XSKQQ.
Enzyme and pathway databases
BRENDA	3.4.21.76. 3474.
Gene expression databases
ArrayExpress	Q61096.
Bgee	Q61096.
CleanEx	MM_PRTN3.
Genevestigator	Q61096.
GermOnline	ENSMUSG00000057729. Mus musculus.
Family and domain databases
InterPro	IPR001254. Peptidase_S1. IPR018114. Peptidase_S1_AS. IPR001314. Peptidase_S1A. IPR009003. Trypsin-like_Pept_dom. [Graphical view]
Pfam	PF00089. Trypsin. 1 hit. [Graphical view]
PRINTS	PR00722. CHYMOTRYPSIN.
SMART	SM00020. Tryp_SPc. 1 hit. [Graphical view]
SUPFAM	SSF50494. Pept_Ser_Cys. 1 hit.
PROSITE	PS50240. TRYPSIN_DOM. 1 hit. PS00134. TRYPSIN_HIS. 1 hit. PS00135. TRYPSIN_SER. 1 hit. [Graphical view]
ProtoNet	Search...
Other
BindingDB	Q61096.
ChEMBL	CHEMBL5384.
ChiTaRS	PRTN3. mouse.
NextBio	295792.
SOURCE	Search...

Entry information

Entry name

PRTN3_MOUSE

Accession

Primary (citable) accession number: Q61096
Secondary accession number(s): O08809

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 30, 2002
Last sequence update:	August 1, 1999
Last modified:	April 3, 2013
This is version 95 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program