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Protein

Cytochrome b-245 heavy chain

Gene

Cybb

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Critical component of the membrane-bound oxidase of phagocytes that generates superoxide. It is the terminal component of a respiratory chain that transfers single electrons from cytoplasmic NADPH across the plasma membrane to molecular oxygen on the exterior. Also functions as a voltage-gated proton channel that mediates the H+ currents of resting phagocytes.1 Publication

Cofactori

FADCurated

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi101Iron (heme axial ligand)Curated1
Metal bindingi115Iron (heme axial ligand)Curated1
Metal bindingi209Iron (heme axial ligand)Curated1
Metal bindingi222Iron (heme axial ligand)Curated1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi338 – 344FADSequence analysis7

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ion channel, Oxidoreductase, Voltage-gated channel

Keywords - Biological processi

Electron transport, Ion transport, Transport

Keywords - Ligandi

FAD, Flavoprotein, Heme, Iron, Metal-binding, NADP

Enzyme and pathway databases

ReactomeiR-MMU-1222556. ROS, RNS production in response to bacteria.
R-MMU-1236973. Cross-presentation of particulate exogenous antigens (phagosomes).
R-MMU-3299685. Detoxification of Reactive Oxygen Species.
R-MMU-4420097. VEGFA-VEGFR2 Pathway.
R-MMU-5668599. RHO GTPases Activate NADPH Oxidases.
R-MMU-6798695. Neutrophil degranulation.

Protein family/group databases

PeroxiBasei5957. MmNOx02.

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome b-245 heavy chain (EC:1.-.-.-)
Alternative name(s):
CGD91-phox
Cytochrome b(558) subunit beta
Short name:
Cytochrome b558 subunit beta
Heme-binding membrane glycoprotein gp91phox
Neutrophil cytochrome b 91 kDa polypeptide
gp91-1
gp91-phox
p22 phagocyte B-cytochrome
Gene namesi
Name:Cybb
Synonyms:Cgd
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome X

Organism-specific databases

MGIiMGI:88574. Cybb.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini2 – 8CytoplasmicSequence analysis7
Transmembranei9 – 29HelicalSequence analysisAdd BLAST21
Topological domaini30 – 48ExtracellularSequence analysisAdd BLAST19
Transmembranei49 – 69HelicalSequence analysisAdd BLAST21
Topological domaini70 – 102CytoplasmicSequence analysisAdd BLAST33
Transmembranei103 – 123HelicalSequence analysisAdd BLAST21
Topological domaini124 – 169ExtracellularSequence analysisAdd BLAST46
Transmembranei170 – 190HelicalSequence analysisAdd BLAST21
Topological domaini191 – 200CytoplasmicSequence analysis10
Transmembranei201 – 221HelicalSequence analysisAdd BLAST21
Topological domaini222 – 261ExtracellularSequence analysisAdd BLAST40
Transmembranei262 – 282HelicalSequence analysisAdd BLAST21
Topological domaini283 – 570CytoplasmicSequence analysisAdd BLAST288

GO - Cellular componenti

  • cytoplasm Source: ParkinsonsUK-UCL
  • dendrite Source: Ensembl
  • Golgi apparatus Source: Ensembl
  • integral component of plasma membrane Source: MGI
  • mitochondrion Source: MGI
  • NADPH oxidase complex Source: UniProtKB
  • neuronal cell body Source: Ensembl
  • nuclear envelope Source: Ensembl
  • perinuclear region of cytoplasm Source: Ensembl
  • phagocytic vesicle Source: ParkinsonsUK-UCL
  • plasma membrane Source: MGI
  • rough endoplasmic reticulum Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00002101462 – 570Cytochrome b-245 heavy chainAdd BLAST569

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi40N-linked (GlcNAc...)Sequence analysis1

Post-translational modificationi

Glycosylated.
Phosphorylated on Ser and Thr residues.By similarity

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiQ61093.
PaxDbiQ61093.
PRIDEiQ61093.

PTM databases

iPTMnetiQ61093.
PhosphoSitePlusiQ61093.

Expressioni

Gene expression databases

BgeeiENSMUSG00000015340.
CleanExiMM_CYBB.
ExpressionAtlasiQ61093. baseline and differential.
GenevisibleiQ61093. MM.

Interactioni

Subunit structurei

Composed of a heavy chain (beta) and a light chain (alpha). Component of an NADPH oxidase complex composed of a heterodimer formed by the membrane proteins CYBA and CYBB and the cytosolic subunits NCF1, NCF2 and NCF4. Interacts with NCF1. Interacts with calprotectin (S100A8/9) (By similarity). Interacts with NRROS; the interaction is direct and impairs formation of a stable NADPH oxidase complex.By similarity1 Publication

GO - Molecular functioni

Protein-protein interaction databases

DIPiDIP-60841N.
IntActiQ61093. 3 interactors.
STRINGi10090.ENSMUSP00000015484.

Structurei

3D structure databases

ProteinModelPortaliQ61093.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini54 – 286Ferric oxidoreductaseAdd BLAST233
Domaini287 – 397FAD-binding FR-typePROSITE-ProRule annotationAdd BLAST111

Sequence similaritiesi

Contains 1 FAD-binding FR-type domain.PROSITE-ProRule annotation
Contains 1 ferric oxidoreductase domain.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0039. Eukaryota.
ENOG410XNZY. LUCA.
GeneTreeiENSGT00550000074350.
HOGENOMiHOG000216669.
HOVERGENiHBG003760.
InParanoidiQ61093.
KOiK08008.
OMAiEPQEAWK.
OrthoDBiEOG091G09RV.
PhylomeDBiQ61093.
TreeFamiTF105354.

Family and domain databases

InterProiIPR000778. Cyt_b245_heavy_chain.
IPR013112. FAD-bd_8.
IPR017927. Fd_Rdtase_FAD-bd.
IPR013130. Fe3_Rdtase_TM_dom.
IPR013121. Fe_red_NAD-bd_6.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view]
PfamiPF08022. FAD_binding_8. 1 hit.
PF01794. Ferric_reduct. 1 hit.
PF08030. NAD_binding_6. 1 hit.
[Graphical view]
PRINTSiPR00466. GP91PHOX.
SUPFAMiSSF63380. SSF63380. 1 hit.
PROSITEiPS51384. FAD_FR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q61093-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGNWAVNEGL SIFVILVWLG LNVFLFINYY KVYDDGPKYN YTRKLLGSAL
60 70 80 90 100
ALARAPAACL NFNCMLILLP VCRNLLSFLR GSSACCSTRI RRQLDRNLTF
110 120 130 140 150
HKMVAWMIAL HTAIHTIAHL FNVEWCVNAR VGISDRYSIA LSDIGDNENE
160 170 180 190 200
EYLNFAREKI KNPEGGLYVA VTRLAGITGI VITLCLILII TSSTKTIRRS
210 220 230 240 250
YFEVFWYTHH LFVIFFIGLA IHGAERIVRG QTAESLEEHN LDICADKIEE
260 270 280 290 300
WGKIKECPVP KFAGNPPMTW KWIVGPMFLY LCERLVRFWR SQQKVVITKV
310 320 330 340 350
VTHPFKTIEL QMKKKGFKME VGQYIFVKCP KVSKLEWHPF TLTSAPEEDF
360 370 380 390 400
FSIHIRIVGD WTEGLFNACG CDKQEFQDAW KLPKIAVDGP FGTASEDVFS
410 420 430 440 450
YEVVMLVGAG IGVTPFASIL KSVWYKYCDN ATSLKLKKIY FYWLCRDTHA
460 470 480 490 500
FEWFADLLQL LETQMQERNN ANFLSYNIYL TGWDESQANH FAVHHDEEKD
510 520 530 540 550
VITGLKQKTL YGRPNWDNEF KTIASEHPNT TIGVFLCGPE ALAETLSKQS
560 570
ISNSESGPRG VHFIFNKENF
Length:570
Mass (Da):65,305
Last modified:November 1, 1996 - v1
Checksum:i8816274E3D253DFA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U43384 mRNA. Translation: AAB05997.1.
BC071229 mRNA. Translation: AAH71229.1.
CCDSiCCDS30010.1.
RefSeqiNP_031833.3. NM_007807.5.
XP_006527628.1. XM_006527565.3.
UniGeneiMm.200362.

Genome annotation databases

EnsembliENSMUST00000015484; ENSMUSP00000015484; ENSMUSG00000015340.
GeneIDi13058.
KEGGimmu:13058.
UCSCiuc009spv.3. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U43384 mRNA. Translation: AAB05997.1.
BC071229 mRNA. Translation: AAH71229.1.
CCDSiCCDS30010.1.
RefSeqiNP_031833.3. NM_007807.5.
XP_006527628.1. XM_006527565.3.
UniGeneiMm.200362.

3D structure databases

ProteinModelPortaliQ61093.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-60841N.
IntActiQ61093. 3 interactors.
STRINGi10090.ENSMUSP00000015484.

Protein family/group databases

PeroxiBasei5957. MmNOx02.

PTM databases

iPTMnetiQ61093.
PhosphoSitePlusiQ61093.

Proteomic databases

MaxQBiQ61093.
PaxDbiQ61093.
PRIDEiQ61093.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000015484; ENSMUSP00000015484; ENSMUSG00000015340.
GeneIDi13058.
KEGGimmu:13058.
UCSCiuc009spv.3. mouse.

Organism-specific databases

CTDi1536.
MGIiMGI:88574. Cybb.

Phylogenomic databases

eggNOGiKOG0039. Eukaryota.
ENOG410XNZY. LUCA.
GeneTreeiENSGT00550000074350.
HOGENOMiHOG000216669.
HOVERGENiHBG003760.
InParanoidiQ61093.
KOiK08008.
OMAiEPQEAWK.
OrthoDBiEOG091G09RV.
PhylomeDBiQ61093.
TreeFamiTF105354.

Enzyme and pathway databases

ReactomeiR-MMU-1222556. ROS, RNS production in response to bacteria.
R-MMU-1236973. Cross-presentation of particulate exogenous antigens (phagosomes).
R-MMU-3299685. Detoxification of Reactive Oxygen Species.
R-MMU-4420097. VEGFA-VEGFR2 Pathway.
R-MMU-5668599. RHO GTPases Activate NADPH Oxidases.
R-MMU-6798695. Neutrophil degranulation.

Miscellaneous databases

ChiTaRSiCybb. mouse.
PROiQ61093.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000015340.
CleanExiMM_CYBB.
ExpressionAtlasiQ61093. baseline and differential.
GenevisibleiQ61093. MM.

Family and domain databases

InterProiIPR000778. Cyt_b245_heavy_chain.
IPR013112. FAD-bd_8.
IPR017927. Fd_Rdtase_FAD-bd.
IPR013130. Fe3_Rdtase_TM_dom.
IPR013121. Fe_red_NAD-bd_6.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view]
PfamiPF08022. FAD_binding_8. 1 hit.
PF01794. Ferric_reduct. 1 hit.
PF08030. NAD_binding_6. 1 hit.
[Graphical view]
PRINTSiPR00466. GP91PHOX.
SUPFAMiSSF63380. SSF63380. 1 hit.
PROSITEiPS51384. FAD_FR. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCY24B_MOUSE
AccessioniPrimary (citable) accession number: Q61093
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 1, 1996
Last modified: November 30, 2016
This is version 136 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.