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Q61093

- CY24B_MOUSE

UniProt

Q61093 - CY24B_MOUSE

Protein

Cytochrome b-245 heavy chain

Gene

Cybb

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 115 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Critical component of the membrane-bound oxidase of phagocytes that generates superoxide. It is the terminal component of a respiratory chain that transfers single electrons from cytoplasmic NADPH across the plasma membrane to molecular oxygen on the exterior. Also functions as a voltage-gated proton channel that mediates the H+ currents of resting phagocytes.1 Publication

    Cofactori

    FAD.Curated

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi101 – 1011Iron (heme axial ligand)Curated
    Metal bindingi115 – 1151Iron (heme axial ligand)Curated
    Metal bindingi209 – 2091Iron (heme axial ligand)Curated
    Metal bindingi222 – 2221Iron (heme axial ligand)Curated

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi338 – 3447FADSequence Analysis

    GO - Molecular functioni

    1. electron carrier activity Source: Ensembl
    2. flavin adenine dinucleotide binding Source: Ensembl
    3. heme binding Source: Ensembl
    4. metal ion binding Source: UniProtKB-KW
    5. protein binding Source: UniProtKB
    6. superoxide-generating NADPH oxidase activity Source: MGI
    7. voltage-gated ion channel activity Source: UniProtKB-KW

    GO - Biological processi

    1. hydrogen peroxide biosynthetic process Source: MGI
    2. inflammatory response Source: UniProtKB
    3. response to drug Source: Ensembl
    4. response to nutrient Source: Ensembl
    5. superoxide anion generation Source: Ensembl
    6. superoxide metabolic process Source: UniProtKB

    Keywords - Molecular functioni

    Ion channel, Oxidoreductase, Voltage-gated channel

    Keywords - Biological processi

    Electron transport, Ion transport, Transport

    Keywords - Ligandi

    FAD, Flavoprotein, Heme, Iron, Metal-binding, NADP

    Enzyme and pathway databases

    ReactomeiREACT_198345. Phagosomal maturation (early endosomal stage).
    REACT_199093. Cross-presentation of particulate exogenous antigens (phagosomes).

    Protein family/group databases

    PeroxiBasei5957. MmNOx02.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cytochrome b-245 heavy chain (EC:1.-.-.-)
    Alternative name(s):
    CGD91-phox
    Cytochrome b(558) subunit beta
    Short name:
    Cytochrome b558 subunit beta
    Heme-binding membrane glycoprotein gp91phox
    Neutrophil cytochrome b 91 kDa polypeptide
    gp91-1
    gp91-phox
    p22 phagocyte B-cytochrome
    Gene namesi
    Name:Cybb
    Synonyms:Cgd
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome X

    Organism-specific databases

    MGIiMGI:88574. Cybb.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: MGI
    2. dendrite Source: Ensembl
    3. Golgi apparatus Source: Ensembl
    4. mitochondrion Source: MGI
    5. NADPH oxidase complex Source: UniProtKB
    6. neuronal cell body Source: Ensembl
    7. plasma membrane Source: MGI
    8. rough endoplasmic reticulum Source: Ensembl

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 570569Cytochrome b-245 heavy chainPRO_0000210146Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi40 – 401N-linked (GlcNAc...)Sequence Analysis

    Post-translational modificationi

    Glycosylated.
    Phosphorylated on Ser and Thr residues.By similarity

    Keywords - PTMi

    Glycoprotein, Phosphoprotein

    Proteomic databases

    MaxQBiQ61093.
    PaxDbiQ61093.
    PRIDEiQ61093.

    PTM databases

    PhosphoSiteiQ61093.

    Expressioni

    Gene expression databases

    ArrayExpressiQ61093.
    BgeeiQ61093.
    CleanExiMM_CYBB.
    GenevestigatoriQ61093.

    Interactioni

    Subunit structurei

    Composed of a heavy chain (beta) and a light chain (alpha). Component of an NADPH oxidase complex composed of a heterodimer formed by the membrane proteins CYBA and CYBB and the cytosolic subunits NCF1, NCF2 and NCF4. Interacts with NCF1. Interacts with calprotectin (S100A8/9) By similarity. Interacts with NRROS; the interaction is direct and impairs formation of a stable NADPH oxidase complex.By similarity1 Publication

    Protein-protein interaction databases

    DIPiDIP-60841N.
    IntActiQ61093. 3 interactions.

    Structurei

    3D structure databases

    ProteinModelPortaliQ61093.
    SMRiQ61093. Positions 385-570.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini2 – 87CytoplasmicSequence Analysis
    Topological domaini30 – 4819ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini70 – 10233CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini124 – 16946ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini191 – 20010CytoplasmicSequence Analysis
    Topological domaini222 – 26140ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini283 – 570288CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei9 – 2921HelicalSequence AnalysisAdd
    BLAST
    Transmembranei49 – 6921HelicalSequence AnalysisAdd
    BLAST
    Transmembranei103 – 12321HelicalSequence AnalysisAdd
    BLAST
    Transmembranei170 – 19021HelicalSequence AnalysisAdd
    BLAST
    Transmembranei201 – 22121HelicalSequence AnalysisAdd
    BLAST
    Transmembranei262 – 28221HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini54 – 286233Ferric oxidoreductaseAdd
    BLAST
    Domaini287 – 397111FAD-binding FR-typePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 FAD-binding FR-type domain.PROSITE-ProRule annotation
    Contains 1 ferric oxidoreductase domain.Curated

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG287712.
    GeneTreeiENSGT00550000074350.
    HOGENOMiHOG000216669.
    HOVERGENiHBG003760.
    InParanoidiQ61093.
    KOiK08008.
    OMAiERNNANF.
    OrthoDBiEOG71P299.
    PhylomeDBiQ61093.
    TreeFamiTF105354.

    Family and domain databases

    InterProiIPR000778. Cyt_b245_heavy_chain.
    IPR013112. FAD-bd_8.
    IPR017927. Fd_Rdtase_FAD-bd.
    IPR013130. Fe3_Rdtase_TM_dom.
    IPR013121. Fe_red_NAD-bd_6.
    IPR017938. Riboflavin_synthase-like_b-brl.
    [Graphical view]
    PfamiPF08022. FAD_binding_8. 1 hit.
    PF01794. Ferric_reduct. 1 hit.
    PF08030. NAD_binding_6. 1 hit.
    [Graphical view]
    PRINTSiPR00466. GP91PHOX.
    SUPFAMiSSF63380. SSF63380. 1 hit.
    PROSITEiPS51384. FAD_FR. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q61093-1 [UniParc]FASTAAdd to Basket

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    MGNWAVNEGL SIFVILVWLG LNVFLFINYY KVYDDGPKYN YTRKLLGSAL    50
    ALARAPAACL NFNCMLILLP VCRNLLSFLR GSSACCSTRI RRQLDRNLTF 100
    HKMVAWMIAL HTAIHTIAHL FNVEWCVNAR VGISDRYSIA LSDIGDNENE 150
    EYLNFAREKI KNPEGGLYVA VTRLAGITGI VITLCLILII TSSTKTIRRS 200
    YFEVFWYTHH LFVIFFIGLA IHGAERIVRG QTAESLEEHN LDICADKIEE 250
    WGKIKECPVP KFAGNPPMTW KWIVGPMFLY LCERLVRFWR SQQKVVITKV 300
    VTHPFKTIEL QMKKKGFKME VGQYIFVKCP KVSKLEWHPF TLTSAPEEDF 350
    FSIHIRIVGD WTEGLFNACG CDKQEFQDAW KLPKIAVDGP FGTASEDVFS 400
    YEVVMLVGAG IGVTPFASIL KSVWYKYCDN ATSLKLKKIY FYWLCRDTHA 450
    FEWFADLLQL LETQMQERNN ANFLSYNIYL TGWDESQANH FAVHHDEEKD 500
    VITGLKQKTL YGRPNWDNEF KTIASEHPNT TIGVFLCGPE ALAETLSKQS 550
    ISNSESGPRG VHFIFNKENF 570
    Length:570
    Mass (Da):65,305
    Last modified:November 1, 1996 - v1
    Checksum:i8816274E3D253DFA
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U43384 mRNA. Translation: AAB05997.1.
    BC071229 mRNA. Translation: AAH71229.1.
    CCDSiCCDS30010.1.
    RefSeqiNP_031833.3. NM_007807.5.
    XP_006527628.1. XM_006527565.1.
    UniGeneiMm.200362.

    Genome annotation databases

    EnsembliENSMUST00000015484; ENSMUSP00000015484; ENSMUSG00000015340.
    GeneIDi13058.
    KEGGimmu:13058.
    UCSCiuc009spv.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U43384 mRNA. Translation: AAB05997.1 .
    BC071229 mRNA. Translation: AAH71229.1 .
    CCDSi CCDS30010.1.
    RefSeqi NP_031833.3. NM_007807.5.
    XP_006527628.1. XM_006527565.1.
    UniGenei Mm.200362.

    3D structure databases

    ProteinModelPortali Q61093.
    SMRi Q61093. Positions 385-570.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-60841N.
    IntActi Q61093. 3 interactions.

    Protein family/group databases

    PeroxiBasei 5957. MmNOx02.

    PTM databases

    PhosphoSitei Q61093.

    Proteomic databases

    MaxQBi Q61093.
    PaxDbi Q61093.
    PRIDEi Q61093.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000015484 ; ENSMUSP00000015484 ; ENSMUSG00000015340 .
    GeneIDi 13058.
    KEGGi mmu:13058.
    UCSCi uc009spv.2. mouse.

    Organism-specific databases

    CTDi 1536.
    MGIi MGI:88574. Cybb.

    Phylogenomic databases

    eggNOGi NOG287712.
    GeneTreei ENSGT00550000074350.
    HOGENOMi HOG000216669.
    HOVERGENi HBG003760.
    InParanoidi Q61093.
    KOi K08008.
    OMAi ERNNANF.
    OrthoDBi EOG71P299.
    PhylomeDBi Q61093.
    TreeFami TF105354.

    Enzyme and pathway databases

    Reactomei REACT_198345. Phagosomal maturation (early endosomal stage).
    REACT_199093. Cross-presentation of particulate exogenous antigens (phagosomes).

    Miscellaneous databases

    ChiTaRSi CYBB. mouse.
    NextBioi 282986.
    PROi Q61093.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q61093.
    Bgeei Q61093.
    CleanExi MM_CYBB.
    Genevestigatori Q61093.

    Family and domain databases

    InterProi IPR000778. Cyt_b245_heavy_chain.
    IPR013112. FAD-bd_8.
    IPR017927. Fd_Rdtase_FAD-bd.
    IPR013130. Fe3_Rdtase_TM_dom.
    IPR013121. Fe_red_NAD-bd_6.
    IPR017938. Riboflavin_synthase-like_b-brl.
    [Graphical view ]
    Pfami PF08022. FAD_binding_8. 1 hit.
    PF01794. Ferric_reduct. 1 hit.
    PF08030. NAD_binding_6. 1 hit.
    [Graphical view ]
    PRINTSi PR00466. GP91PHOX.
    SUPFAMi SSF63380. SSF63380. 1 hit.
    PROSITEi PS51384. FAD_FR. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning of murine gp91phox cDNA and functional expression in a human X-linked chronic granulomatous disease cell line."
      Bjorgvinsdottir H., Zhen L., Dinauer M.C.
      Blood 87:2005-2010(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: NMRI.
      Tissue: Mammary gland.
    3. Cited for: INTERACTION WITH NRROS, FUNCTION.

    Entry informationi

    Entry nameiCY24B_MOUSE
    AccessioniPrimary (citable) accession number: Q61093
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 30, 2000
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 115 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3