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Q61093

- CY24B_MOUSE

UniProt

Q61093 - CY24B_MOUSE

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Protein

Cytochrome b-245 heavy chain

Gene

Cybb

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Critical component of the membrane-bound oxidase of phagocytes that generates superoxide. It is the terminal component of a respiratory chain that transfers single electrons from cytoplasmic NADPH across the plasma membrane to molecular oxygen on the exterior. Also functions as a voltage-gated proton channel that mediates the H+ currents of resting phagocytes.1 Publication

Cofactori

FAD.Curated

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi101 – 1011Iron (heme axial ligand)Curated
Metal bindingi115 – 1151Iron (heme axial ligand)Curated
Metal bindingi209 – 2091Iron (heme axial ligand)Curated
Metal bindingi222 – 2221Iron (heme axial ligand)Curated

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi338 – 3447FADSequence Analysis

GO - Molecular functioni

  1. electron carrier activity Source: Ensembl
  2. flavin adenine dinucleotide binding Source: Ensembl
  3. heme binding Source: Ensembl
  4. metal ion binding Source: UniProtKB-KW
  5. superoxide-generating NADPH oxidase activity Source: MGI
  6. voltage-gated ion channel activity Source: UniProtKB-KW

GO - Biological processi

  1. hydrogen peroxide biosynthetic process Source: MGI
  2. inflammatory response Source: UniProtKB
  3. response to drug Source: Ensembl
  4. response to nutrient Source: Ensembl
  5. superoxide anion generation Source: Ensembl
  6. superoxide metabolic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Ion channel, Oxidoreductase, Voltage-gated channel

Keywords - Biological processi

Electron transport, Ion transport, Transport

Keywords - Ligandi

FAD, Flavoprotein, Heme, Iron, Metal-binding, NADP

Enzyme and pathway databases

ReactomeiREACT_198345. Phagosomal maturation (early endosomal stage).
REACT_199093. Cross-presentation of particulate exogenous antigens (phagosomes).

Protein family/group databases

PeroxiBasei5957. MmNOx02.

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome b-245 heavy chain (EC:1.-.-.-)
Alternative name(s):
CGD91-phox
Cytochrome b(558) subunit beta
Short name:
Cytochrome b558 subunit beta
Heme-binding membrane glycoprotein gp91phox
Neutrophil cytochrome b 91 kDa polypeptide
gp91-1
gp91-phox
p22 phagocyte B-cytochrome
Gene namesi
Name:Cybb
Synonyms:Cgd
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome X

Organism-specific databases

MGIiMGI:88574. Cybb.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: MGI
  2. dendrite Source: Ensembl
  3. Golgi apparatus Source: Ensembl
  4. mitochondrion Source: MGI
  5. NADPH oxidase complex Source: UniProtKB
  6. neuronal cell body Source: Ensembl
  7. plasma membrane Source: MGI
  8. rough endoplasmic reticulum Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 570569Cytochrome b-245 heavy chainPRO_0000210146Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi40 – 401N-linked (GlcNAc...)Sequence Analysis

Post-translational modificationi

Glycosylated.
Phosphorylated on Ser and Thr residues.By similarity

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiQ61093.
PaxDbiQ61093.
PRIDEiQ61093.

PTM databases

PhosphoSiteiQ61093.

Expressioni

Gene expression databases

BgeeiQ61093.
CleanExiMM_CYBB.
ExpressionAtlasiQ61093. baseline and differential.
GenevestigatoriQ61093.

Interactioni

Subunit structurei

Composed of a heavy chain (beta) and a light chain (alpha). Component of an NADPH oxidase complex composed of a heterodimer formed by the membrane proteins CYBA and CYBB and the cytosolic subunits NCF1, NCF2 and NCF4. Interacts with NCF1. Interacts with calprotectin (S100A8/9) (By similarity). Interacts with NRROS; the interaction is direct and impairs formation of a stable NADPH oxidase complex.By similarity1 Publication

Protein-protein interaction databases

DIPiDIP-60841N.
IntActiQ61093. 3 interactions.

Structurei

3D structure databases

ProteinModelPortaliQ61093.
SMRiQ61093. Positions 385-570.
ModBaseiSearch...
MobiDBiSearch...

Topological domain

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini2 – 87CytoplasmicSequence Analysis
Topological domaini30 – 4819ExtracellularSequence AnalysisAdd
BLAST
Topological domaini70 – 10233CytoplasmicSequence AnalysisAdd
BLAST
Topological domaini124 – 16946ExtracellularSequence AnalysisAdd
BLAST
Topological domaini191 – 20010CytoplasmicSequence Analysis
Topological domaini222 – 26140ExtracellularSequence AnalysisAdd
BLAST
Topological domaini283 – 570288CytoplasmicSequence AnalysisAdd
BLAST

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei9 – 2921HelicalSequence AnalysisAdd
BLAST
Transmembranei49 – 6921HelicalSequence AnalysisAdd
BLAST
Transmembranei103 – 12321HelicalSequence AnalysisAdd
BLAST
Transmembranei170 – 19021HelicalSequence AnalysisAdd
BLAST
Transmembranei201 – 22121HelicalSequence AnalysisAdd
BLAST
Transmembranei262 – 28221HelicalSequence AnalysisAdd
BLAST

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini54 – 286233Ferric oxidoreductaseAdd
BLAST
Domaini287 – 397111FAD-binding FR-typePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 FAD-binding FR-type domain.PROSITE-ProRule annotation
Contains 1 ferric oxidoreductase domain.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG287712.
GeneTreeiENSGT00550000074350.
HOGENOMiHOG000216669.
HOVERGENiHBG003760.
InParanoidiQ61093.
KOiK08008.
OMAiERNNANF.
OrthoDBiEOG71P299.
PhylomeDBiQ61093.
TreeFamiTF105354.

Family and domain databases

InterProiIPR000778. Cyt_b245_heavy_chain.
IPR013112. FAD-bd_8.
IPR017927. Fd_Rdtase_FAD-bd.
IPR013130. Fe3_Rdtase_TM_dom.
IPR013121. Fe_red_NAD-bd_6.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view]
PfamiPF08022. FAD_binding_8. 1 hit.
PF01794. Ferric_reduct. 1 hit.
PF08030. NAD_binding_6. 1 hit.
[Graphical view]
PRINTSiPR00466. GP91PHOX.
SUPFAMiSSF63380. SSF63380. 1 hit.
PROSITEiPS51384. FAD_FR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q61093-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MGNWAVNEGL SIFVILVWLG LNVFLFINYY KVYDDGPKYN YTRKLLGSAL
60 70 80 90 100
ALARAPAACL NFNCMLILLP VCRNLLSFLR GSSACCSTRI RRQLDRNLTF
110 120 130 140 150
HKMVAWMIAL HTAIHTIAHL FNVEWCVNAR VGISDRYSIA LSDIGDNENE
160 170 180 190 200
EYLNFAREKI KNPEGGLYVA VTRLAGITGI VITLCLILII TSSTKTIRRS
210 220 230 240 250
YFEVFWYTHH LFVIFFIGLA IHGAERIVRG QTAESLEEHN LDICADKIEE
260 270 280 290 300
WGKIKECPVP KFAGNPPMTW KWIVGPMFLY LCERLVRFWR SQQKVVITKV
310 320 330 340 350
VTHPFKTIEL QMKKKGFKME VGQYIFVKCP KVSKLEWHPF TLTSAPEEDF
360 370 380 390 400
FSIHIRIVGD WTEGLFNACG CDKQEFQDAW KLPKIAVDGP FGTASEDVFS
410 420 430 440 450
YEVVMLVGAG IGVTPFASIL KSVWYKYCDN ATSLKLKKIY FYWLCRDTHA
460 470 480 490 500
FEWFADLLQL LETQMQERNN ANFLSYNIYL TGWDESQANH FAVHHDEEKD
510 520 530 540 550
VITGLKQKTL YGRPNWDNEF KTIASEHPNT TIGVFLCGPE ALAETLSKQS
560 570
ISNSESGPRG VHFIFNKENF
Length:570
Mass (Da):65,305
Last modified:November 1, 1996 - v1
Checksum:i8816274E3D253DFA
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U43384 mRNA. Translation: AAB05997.1.
BC071229 mRNA. Translation: AAH71229.1.
CCDSiCCDS30010.1.
RefSeqiNP_031833.3. NM_007807.5.
XP_006527628.1. XM_006527565.1.
UniGeneiMm.200362.

Genome annotation databases

EnsembliENSMUST00000015484; ENSMUSP00000015484; ENSMUSG00000015340.
GeneIDi13058.
KEGGimmu:13058.
UCSCiuc009spv.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U43384 mRNA. Translation: AAB05997.1 .
BC071229 mRNA. Translation: AAH71229.1 .
CCDSi CCDS30010.1.
RefSeqi NP_031833.3. NM_007807.5.
XP_006527628.1. XM_006527565.1.
UniGenei Mm.200362.

3D structure databases

ProteinModelPortali Q61093.
SMRi Q61093. Positions 385-570.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-60841N.
IntActi Q61093. 3 interactions.

Protein family/group databases

PeroxiBasei 5957. MmNOx02.

PTM databases

PhosphoSitei Q61093.

Proteomic databases

MaxQBi Q61093.
PaxDbi Q61093.
PRIDEi Q61093.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000015484 ; ENSMUSP00000015484 ; ENSMUSG00000015340 .
GeneIDi 13058.
KEGGi mmu:13058.
UCSCi uc009spv.2. mouse.

Organism-specific databases

CTDi 1536.
MGIi MGI:88574. Cybb.

Phylogenomic databases

eggNOGi NOG287712.
GeneTreei ENSGT00550000074350.
HOGENOMi HOG000216669.
HOVERGENi HBG003760.
InParanoidi Q61093.
KOi K08008.
OMAi ERNNANF.
OrthoDBi EOG71P299.
PhylomeDBi Q61093.
TreeFami TF105354.

Enzyme and pathway databases

Reactomei REACT_198345. Phagosomal maturation (early endosomal stage).
REACT_199093. Cross-presentation of particulate exogenous antigens (phagosomes).

Miscellaneous databases

ChiTaRSi CYBB. mouse.
NextBioi 282986.
PROi Q61093.
SOURCEi Search...

Gene expression databases

Bgeei Q61093.
CleanExi MM_CYBB.
ExpressionAtlasi Q61093. baseline and differential.
Genevestigatori Q61093.

Family and domain databases

InterProi IPR000778. Cyt_b245_heavy_chain.
IPR013112. FAD-bd_8.
IPR017927. Fd_Rdtase_FAD-bd.
IPR013130. Fe3_Rdtase_TM_dom.
IPR013121. Fe_red_NAD-bd_6.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view ]
Pfami PF08022. FAD_binding_8. 1 hit.
PF01794. Ferric_reduct. 1 hit.
PF08030. NAD_binding_6. 1 hit.
[Graphical view ]
PRINTSi PR00466. GP91PHOX.
SUPFAMi SSF63380. SSF63380. 1 hit.
PROSITEi PS51384. FAD_FR. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning of murine gp91phox cDNA and functional expression in a human X-linked chronic granulomatous disease cell line."
    Bjorgvinsdottir H., Zhen L., Dinauer M.C.
    Blood 87:2005-2010(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: NMRI.
    Tissue: Mammary gland.
  3. Cited for: INTERACTION WITH NRROS, FUNCTION.

Entry informationi

Entry nameiCY24B_MOUSE
AccessioniPrimary (citable) accession number: Q61093
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 1, 1996
Last modified: October 29, 2014
This is version 116 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3