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Protein

Cytochrome b-245 heavy chain

Gene

Cybb

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Critical component of the membrane-bound oxidase of phagocytes that generates superoxide. It is the terminal component of a respiratory chain that transfers single electrons from cytoplasmic NADPH across the plasma membrane to molecular oxygen on the exterior. Also functions as a voltage-gated proton channel that mediates the H+ currents of resting phagocytes.1 Publication

Cofactori

FADCurated

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi101Iron (heme axial ligand)Curated1
Metal bindingi115Iron (heme axial ligand)Curated1
Metal bindingi209Iron (heme axial ligand)Curated1
Metal bindingi222Iron (heme axial ligand)Curated1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi338 – 344FADSequence analysis7

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionIon channel, Oxidoreductase, Voltage-gated channel
Biological processElectron transport, Ion transport, Transport
LigandFAD, Flavoprotein, Heme, Iron, Metal-binding, NADP

Enzyme and pathway databases

ReactomeiR-MMU-1222556 ROS, RNS production in phagocytes
R-MMU-1236973 Cross-presentation of particulate exogenous antigens (phagosomes)
R-MMU-3299685 Detoxification of Reactive Oxygen Species
R-MMU-4420097 VEGFA-VEGFR2 Pathway
R-MMU-5668599 RHO GTPases Activate NADPH Oxidases
R-MMU-6798695 Neutrophil degranulation

Protein family/group databases

PeroxiBasei5957 MmNOx02

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome b-245 heavy chain (EC:1.-.-.-)
Alternative name(s):
CGD91-phox
Cytochrome b(558) subunit beta
Short name:
Cytochrome b558 subunit beta
Heme-binding membrane glycoprotein gp91phox
Neutrophil cytochrome b 91 kDa polypeptide
gp91-1
gp91-phox
p22 phagocyte B-cytochrome
Gene namesi
Name:CybbImported
Synonyms:Cgd
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome X

Organism-specific databases

MGIiMGI:88574 Cybb

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 8CytoplasmicSequence analysis8
Transmembranei9 – 29HelicalSequence analysisAdd BLAST21
Topological domaini30 – 48ExtracellularSequence analysisAdd BLAST19
Transmembranei49 – 69HelicalSequence analysisAdd BLAST21
Topological domaini70 – 102CytoplasmicSequence analysisAdd BLAST33
Transmembranei103 – 123HelicalSequence analysisAdd BLAST21
Topological domaini124 – 169ExtracellularSequence analysisAdd BLAST46
Transmembranei170 – 190HelicalSequence analysisAdd BLAST21
Topological domaini191 – 200CytoplasmicSequence analysis10
Transmembranei201 – 221HelicalSequence analysisAdd BLAST21
Topological domaini222 – 261ExtracellularSequence analysisAdd BLAST40
Transmembranei262 – 282HelicalSequence analysisAdd BLAST21
Topological domaini283 – 570CytoplasmicSequence analysisAdd BLAST288

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002101461 – 570Cytochrome b-245 heavy chainAdd BLAST570

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi40N-linked (GlcNAc...) asparagineSequence analysis1
Cross-linki159Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication
Cross-linki161Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication
Cross-linki255Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication
Cross-linki294Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication
Cross-linki299Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication
Cross-linki306Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication
Cross-linki328Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication
Cross-linki334Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication
Cross-linki381Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication
Cross-linki506Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication
Cross-linki567Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication

Post-translational modificationi

Glycosylated.
Phosphorylated on Ser and Thr residues.By similarity
Undergoes 'Lys-48'-linked polyubiquitination, likely by RNF145, triggering endoplasmic reticulum-associated degradation.1 Publication

Keywords - PTMi

Glycoprotein, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiQ61093
PaxDbiQ61093
PRIDEiQ61093

PTM databases

iPTMnetiQ61093
PhosphoSitePlusiQ61093
SwissPalmiQ61093

Expressioni

Gene expression databases

BgeeiENSMUSG00000015340
CleanExiMM_CYBB
ExpressionAtlasiQ61093 baseline and differential
GenevisibleiQ61093 MM

Interactioni

Subunit structurei

Composed of a heavy chain (beta) and a light chain (alpha). Component of an NADPH oxidase complex composed of a heterodimer formed by the membrane proteins CYBA and CYBB and the cytosolic subunits NCF1, NCF2 and NCF4. Interacts with NCF1. Interacts with calprotectin (S100A8/9) (By similarity). Interacts with NRROS; the interaction is direct and impairs formation of a stable NADPH oxidase complex.By similarity1 Publication

Binary interactionsi

Show more details

GO - Molecular functioni

Protein-protein interaction databases

BioGridi198990, 1 interactor
DIPiDIP-60841N
IntActiQ61093, 5 interactors
STRINGi10090.ENSMUSP00000015484

Structurei

3D structure databases

ProteinModelPortaliQ61093
SMRiQ61093
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini54 – 286Ferric oxidoreductaseAdd BLAST233
Domaini287 – 397FAD-binding FR-typePROSITE-ProRule annotationAdd BLAST111

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0039 Eukaryota
ENOG410XNZY LUCA
GeneTreeiENSGT00550000074350
HOGENOMiHOG000216669
HOVERGENiHBG003760
InParanoidiQ61093
KOiK21421
OMAiKAGQWLF
OrthoDBiEOG091G09RV
PhylomeDBiQ61093
TreeFamiTF105354

Family and domain databases

InterProiView protein in InterPro
IPR000778 Cyt_b245_heavy_chain
IPR013112 FAD-bd_8
IPR017927 Fd_Rdtase_FAD-bd
IPR013130 Fe3_Rdtase_TM_dom
IPR013121 Fe_red_NAD-bd_6
IPR017938 Riboflavin_synthase-like_b-brl
PfamiView protein in Pfam
PF08022 FAD_binding_8, 1 hit
PF01794 Ferric_reduct, 1 hit
PF08030 NAD_binding_6, 1 hit
PRINTSiPR00466 GP91PHOX
SUPFAMiSSF63380 SSF63380, 1 hit
PROSITEiView protein in PROSITE
PS51384 FAD_FR, 1 hit

Sequencei

Sequence statusi: Complete.

Q61093-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGNWAVNEGL SIFVILVWLG LNVFLFINYY KVYDDGPKYN YTRKLLGSAL
60 70 80 90 100
ALARAPAACL NFNCMLILLP VCRNLLSFLR GSSACCSTRI RRQLDRNLTF
110 120 130 140 150
HKMVAWMIAL HTAIHTIAHL FNVEWCVNAR VGISDRYSIA LSDIGDNENE
160 170 180 190 200
EYLNFAREKI KNPEGGLYVA VTRLAGITGI VITLCLILII TSSTKTIRRS
210 220 230 240 250
YFEVFWYTHH LFVIFFIGLA IHGAERIVRG QTAESLEEHN LDICADKIEE
260 270 280 290 300
WGKIKECPVP KFAGNPPMTW KWIVGPMFLY LCERLVRFWR SQQKVVITKV
310 320 330 340 350
VTHPFKTIEL QMKKKGFKME VGQYIFVKCP KVSKLEWHPF TLTSAPEEDF
360 370 380 390 400
FSIHIRIVGD WTEGLFNACG CDKQEFQDAW KLPKIAVDGP FGTASEDVFS
410 420 430 440 450
YEVVMLVGAG IGVTPFASIL KSVWYKYCDN ATSLKLKKIY FYWLCRDTHA
460 470 480 490 500
FEWFADLLQL LETQMQERNN ANFLSYNIYL TGWDESQANH FAVHHDEEKD
510 520 530 540 550
VITGLKQKTL YGRPNWDNEF KTIASEHPNT TIGVFLCGPE ALAETLSKQS
560 570
ISNSESGPRG VHFIFNKENF
Length:570
Mass (Da):65,305
Last modified:November 1, 1996 - v1
Checksum:i8816274E3D253DFA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U43384 mRNA Translation: AAB05997.1
BC071229 mRNA Translation: AAH71229.1
CCDSiCCDS30010.1
RefSeqiNP_031833.3, NM_007807.5
XP_006527628.1, XM_006527565.3
UniGeneiMm.200362

Genome annotation databases

EnsembliENSMUST00000015484; ENSMUSP00000015484; ENSMUSG00000015340
GeneIDi13058
KEGGimmu:13058
UCSCiuc009spv.3 mouse

Similar proteinsi

Entry informationi

Entry nameiCY24B_MOUSE
AccessioniPrimary (citable) accession number: Q61093
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 1, 1996
Last modified: June 20, 2018
This is version 149 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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