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Q61090 (FZD7_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 112. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Frizzled-7

Short name=Fz-7
Short name=mFz7
Gene names
Name:Fzd7
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length572 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues. Activation by Wnt8 induces expression of beta-catenin target genes. Ref.5 Ref.6

Subunit structure

Interacts with MAGI3 and DVL1. Interacts with MYOC By similarity. Ref.6 Ref.7

Subcellular location

Membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein Ref.5 Ref.6.

Domain

Lys-Thr-X-X-X-Trp motif interacts with the PDZ doman of Dvl (Disheveled) family members and is involved in the activation of the Wnt/beta-catenin signaling pathway By similarity.

The FZ domain is involved in binding with Wnt ligands By similarity.

Post-translational modification

Ubiquitinated by ZNRF3, leading to its degradation by the proteasome By similarity.

Sequence similarities

Belongs to the G-protein coupled receptor Fz/Smo family.

Contains 1 FZ (frizzled) domain.

Ontologies

Keywords
   Biological processWnt signaling pathway
   Cellular componentCell membrane
Membrane
   DomainSignal
Transmembrane
Transmembrane helix
   Molecular functionDevelopmental protein
G-protein coupled receptor
Receptor
Transducer
   PTMDisulfide bond
Glycoprotein
Ubl conjugation
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processG-protein coupled receptor signaling pathway coupled to cGMP nucleotide second messenger

Inferred from Biological aspect of Ancestor. Source: RefGenome

T cell differentiation in thymus

Inferred from mutant phenotype PubMed 11265645. Source: MGI

Wnt signaling pathway, calcium modulating pathway

Inferred from Biological aspect of Ancestor. Source: RefGenome

axonogenesis

Inferred from Biological aspect of Ancestor. Source: RefGenome

brain development

Inferred from Biological aspect of Ancestor. Source: RefGenome

canonical Wnt signaling pathway

Inferred from mutant phenotype PubMed 11265645. Source: MGI

embryo development

Inferred from Biological aspect of Ancestor. Source: RefGenome

epithelial cell differentiation

Inferred from Biological aspect of Ancestor. Source: RefGenome

gonad development

Inferred from Biological aspect of Ancestor. Source: RefGenome

mesenchymal to epithelial transition

Inferred from electronic annotation. Source: Ensembl

negative regulation of cell-substrate adhesion

Inferred from mutant phenotype PubMed 18156211. Source: BHF-UCL

negative regulation of ectodermal cell fate specification

Inferred from electronic annotation. Source: Ensembl

non-canonical Wnt signaling pathway

Inferred from genetic interaction PubMed 19497282. Source: MGI

non-canonical Wnt signaling pathway via JNK cascade

Inferred from electronic annotation. Source: Ensembl

positive regulation of epithelial cell proliferation involved in wound healing

Inferred from electronic annotation. Source: Ensembl

positive regulation of phosphorylation

Inferred from electronic annotation. Source: Ensembl

positive regulation of transcription, DNA-templated

Inferred from electronic annotation. Source: Ensembl

regulation of catenin import into nucleus

Inferred from electronic annotation. Source: Ensembl

regulation of transcription from RNA polymerase II promoter

Inferred from Biological aspect of Ancestor. Source: RefGenome

satellite cell maintenance involved in skeletal muscle regeneration

Inferred from mutant phenotype PubMed 19497282. Source: MGI

somatic stem cell division

Inferred from mutant phenotype PubMed 19497282. Source: MGI

substrate adhesion-dependent cell spreading

Inferred from mutant phenotype PubMed 18156211. Source: MGI

vasculature development

Inferred from Biological aspect of Ancestor. Source: RefGenome

   Cellular_componentapical part of cell

Inferred from Biological aspect of Ancestor. Source: RefGenome

cytoplasm

Inferred from Biological aspect of Ancestor. Source: RefGenome

integral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

neuron projection membrane

Inferred from Biological aspect of Ancestor. Source: RefGenome

plasma membrane

Inferred from mutant phenotype PubMed 18156211. Source: BHF-UCL

   Molecular_functionG-protein coupled receptor activity

Inferred from electronic annotation. Source: UniProtKB-KW

PDZ domain binding

Inferred from Biological aspect of Ancestor. Source: RefGenome

Wnt-activated receptor activity

Inferred from mutant phenotype PubMed 19497282. Source: MGI

Wnt-protein binding

Inferred from physical interaction PubMed 19497282. Source: MGI

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Dlg4Q621084EBI-8473104,EBI-300895

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 3232 Potential
Chain33 – 572540Frizzled-7
PRO_0000012997

Regions

Topological domain33 – 254222Extracellular Potential
Transmembrane255 – 27521Helical; Name=1; Potential
Topological domain276 – 28611Cytoplasmic Potential
Transmembrane287 – 30721Helical; Name=2; Potential
Topological domain308 – 33427Extracellular Potential
Transmembrane335 – 35521Helical; Name=3; Potential
Topological domain356 – 37722Cytoplasmic Potential
Transmembrane378 – 39821Helical; Name=4; Potential
Topological domain399 – 42123Extracellular Potential
Transmembrane422 – 44221Helical; Name=5; Potential
Topological domain443 – 46826Cytoplasmic Potential
Transmembrane469 – 48921Helical; Name=6; Potential
Topological domain490 – 52637Extracellular Potential
Transmembrane527 – 54721Helical; Name=7; Potential
Topological domain548 – 57225Cytoplasmic Potential
Domain44 – 163120FZ
Motif550 – 5556Lys-Thr-X-X-X-Trp motif, mediates interaction with the PDZ domain of Dvl family members By similarity
Motif570 – 5723PDZ-binding

Amino acid modifications

Glycosylation631N-linked (GlcNAc...) Potential
Glycosylation1641N-linked (GlcNAc...) Potential
Disulfide bond49 ↔ 110 By similarity
Disulfide bond57 ↔ 103 By similarity
Disulfide bond94 ↔ 131 By similarity
Disulfide bond120 ↔ 160 By similarity
Disulfide bond124 ↔ 148 By similarity

Experimental info

Sequence conflict2621C → S in AAC52432. Ref.1
Sequence conflict5161G → R in AAC52432. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q61090 [UniParc].

Last modified October 3, 2012. Version 2.
Checksum: D0B7DF37B9736183

FASTA57263,733
        10         20         30         40         50         60 
MRGPGTAASH SPLGLCALVL ALLGALPTDT RAQPYHGEKG ISVPDHGFCQ PISIPLCTDI 

        70         80         90        100        110        120 
AYNQTILPNL LGHTNQEDAG LEVHQFYPLV KVQCSPELRF FLCSMYAPVC TVLDQAIPPC 

       130        140        150        160        170        180 
RSLCERARQG CEALMNKFGF QWPERLRCEN FPVHGAGEIC VGQNTSDGSG GAGGSPTAYP 

       190        200        210        220        230        240 
TAPYLPDPPF TAMSPSDGRG RLSFPFSCPR QLKVPPYLGY RFLGERDCGA PCEPGRANGL 

       250        260        270        280        290        300 
MYFKEEERRF ARLWVGVWSV LCCASTLFTV LTYLVDMRRF SYPERPIIFL SGCYFMVAVA 

       310        320        330        340        350        360 
HVAGFLLEDR AVCVERFSDD GYRTVAQGTK KEGCTILFMV LYFFGMASSI WWVILSLTWF 

       370        380        390        400        410        420 
LAAGMKWGHE AIEANSQYFH LAAWAVPAVK TITILAMGQV DGDLLSGVCY VGLSSVDALR 

       430        440        450        460        470        480 
GFVLAPLFVY LFIGTSFLLA GFVSLFRIRT IMKHDGTKTE KLEKLMVRIG VFSVLYTVPA 

       490        500        510        520        530        540 
TIVLACYFYE QAFREHWERT WLLQTCKSYA VPCPPGHFSP MSPDFTVFMI KYLMTMIVGI 

       550        560        570 
TTGFWIWSGK TLQSWRRFYH RLSHSSKGET AV 

« Hide

References

« Hide 'large scale' references
[1]"A large family of putative transmembrane receptors homologous to the product of the Drosophila tissue polarity gene frizzled."
Wang Y., Macke J.P., Abella B.S., Andreasson K., Worley P., Gilbert D.J., Copeland N.G., Jenkins N.A., Nathans J.
J. Biol. Chem. 271:4468-4476(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[3]Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]"Protein kinase C is differentially stimulated by Wnt and Frizzled homologs in a G-protein-dependent manner."
Sheldahl L.C., Park M., Malbon C.C., Moon R.T.
Curr. Biol. 9:695-698(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: COUPLING TO BETA-CATENIN PATHWAY.
[5]"Biochemical characterization of Wnt-frizzled interactions using a soluble, biologically active vertebrate Wnt protein."
Hsieh J.C., Rattner A., Smallwood P.M., Nathans J.
Proc. Natl. Acad. Sci. U.S.A. 96:3546-3551(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION.
[6]"Characterization of function of three domains in dishevelled-1: DEP domain is responsible for membrane translocation of dishevelled-1."
Pan W.J., Pang S.Z., Huang T., Guo H.Y., Wu D., Li L.
Cell Res. 14:324-330(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH DVL1.
[7]"MAGI-3 is involved in the regulation of the JNK signaling pathway as a scaffold protein for frizzled and Ltap."
Yao R., Natsume Y., Noda T.
Oncogene 23:6023-6030(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH MAGI3.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U43320 mRNA. Translation: AAC52432.1.
AC132574 Genomic DNA. No translation available.
CH466548 Genomic DNA. Translation: EDL00127.1.
RefSeqNP_032083.3. NM_008057.3.
UniGeneMm.297906.

3D structure databases

ProteinModelPortalQ61090.
SMRQ61090. Positions 49-155, 228-562.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActQ61090. 4 interactions.
MINTMINT-1780171.

Protein family/group databases

MEROPSI93.001.
GPCRDBSearch...

PTM databases

PhosphoSiteQ61090.

Proteomic databases

PRIDEQ61090.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000037105; ENSMUSP00000041825; ENSMUSG00000041075.
ENSMUST00000114246; ENSMUSP00000109884; ENSMUSG00000041075.
GeneID14369.
KEGGmmu:14369.
UCSCuc011wlq.1. mouse.

Organism-specific databases

CTD8324.
MGIMGI:108570. Fzd7.

Phylogenomic databases

eggNOGNOG257258.
GeneTreeENSGT00740000114899.
HOVERGENHBG006977.
InParanoidQ61090.
KOK02432.
OMACVERFSE.
OrthoDBEOG7M3J01.
TreeFamTF317907.

Gene expression databases

CleanExMM_FZD7.
GenevestigatorQ61090.

Family and domain databases

Gene3D1.10.2000.10. 1 hit.
InterProIPR000539. Frizzled.
IPR015526. Frizzled/SFRP.
IPR020067. Frizzled_dom.
IPR026552. FZD7.
IPR017981. GPCR_2-like.
[Graphical view]
PANTHERPTHR11309. PTHR11309. 1 hit.
PTHR11309:SF31. PTHR11309:SF31. 1 hit.
PfamPF01534. Frizzled. 1 hit.
PF01392. Fz. 1 hit.
[Graphical view]
PRINTSPR00489. FRIZZLED.
SMARTSM00063. FRI. 1 hit.
[Graphical view]
SUPFAMSSF63501. SSF63501. 1 hit.
PROSITEPS50038. FZ. 1 hit.
PS50261. G_PROTEIN_RECEP_F2_4. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio285849.
PROQ61090.
SOURCESearch...

Entry information

Entry nameFZD7_MOUSE
AccessionPrimary (citable) accession number: Q61090
Secondary accession number(s): G3X953
Entry history
Integrated into UniProtKB/Swiss-Prot: December 5, 2001
Last sequence update: October 3, 2012
Last modified: April 16, 2014
This is version 112 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

7-transmembrane G-linked receptors

List of 7-transmembrane G-linked receptor entries