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Protein

Frizzled-7

Gene

Fzd7

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues. Activation by Wnt8 induces expression of beta-catenin target genes.2 Publications

GO - Molecular functioni

  1. frizzled binding Source: MGI
  2. G-protein coupled receptor activity Source: GO_Central
  3. PDZ domain binding Source: MGI
  4. Wnt-activated receptor activity Source: MGI
  5. Wnt-protein binding Source: MGI

GO - Biological processi

  1. canonical Wnt signaling pathway Source: MGI
  2. mesenchymal to epithelial transition Source: MGI
  3. negative regulation of cell-substrate adhesion Source: BHF-UCL
  4. negative regulation of ectodermal cell fate specification Source: MGI
  5. non-canonical Wnt signaling pathway Source: MGI
  6. non-canonical Wnt signaling pathway via JNK cascade Source: MGI
  7. positive regulation of epithelial cell proliferation involved in wound healing Source: MGI
  8. positive regulation of phosphorylation Source: MGI
  9. positive regulation of transcription, DNA-templated Source: MGI
  10. regulation of catenin import into nucleus Source: MGI
  11. regulation of transcription, DNA-templated Source: MGI
  12. skeletal muscle satellite cell maintenance involved in skeletal muscle regeneration Source: MGI
  13. somatic stem cell division Source: MGI
  14. stem cell maintenance Source: MGI
  15. substrate adhesion-dependent cell spreading Source: MGI
  16. T cell differentiation in thymus Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, G-protein coupled receptor, Receptor, Transducer

Keywords - Biological processi

Wnt signaling pathway

Enzyme and pathway databases

ReactomeiREACT_214043. PCP/CE pathway.
REACT_250061. Class B/2 (Secretin family receptors).

Protein family/group databases

MEROPSiI93.001.

Names & Taxonomyi

Protein namesi
Recommended name:
Frizzled-7
Short name:
Fz-7
Short name:
mFz7
Gene namesi
Name:Fzd7
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 1

Organism-specific databases

MGIiMGI:108570. Fzd7.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini33 – 254222ExtracellularSequence AnalysisAdd
BLAST
Transmembranei255 – 27521Helical; Name=1Sequence AnalysisAdd
BLAST
Topological domaini276 – 28611CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei287 – 30721Helical; Name=2Sequence AnalysisAdd
BLAST
Topological domaini308 – 33427ExtracellularSequence AnalysisAdd
BLAST
Transmembranei335 – 35521Helical; Name=3Sequence AnalysisAdd
BLAST
Topological domaini356 – 37722CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei378 – 39821Helical; Name=4Sequence AnalysisAdd
BLAST
Topological domaini399 – 42123ExtracellularSequence AnalysisAdd
BLAST
Transmembranei422 – 44221Helical; Name=5Sequence AnalysisAdd
BLAST
Topological domaini443 – 46826CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei469 – 48921Helical; Name=6Sequence AnalysisAdd
BLAST
Topological domaini490 – 52637ExtracellularSequence AnalysisAdd
BLAST
Transmembranei527 – 54721Helical; Name=7Sequence AnalysisAdd
BLAST
Topological domaini548 – 57225CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: GO_Central
  2. plasma membrane Source: BHF-UCL
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3232Sequence AnalysisAdd
BLAST
Chaini33 – 572540Frizzled-7PRO_0000012997Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi49 ↔ 110PROSITE-ProRule annotation
Disulfide bondi57 ↔ 103PROSITE-ProRule annotation
Glycosylationi63 – 631N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi94 ↔ 131PROSITE-ProRule annotation
Disulfide bondi120 ↔ 160PROSITE-ProRule annotation
Disulfide bondi124 ↔ 148PROSITE-ProRule annotation
Glycosylationi164 – 1641N-linked (GlcNAc...)Sequence Analysis

Post-translational modificationi

Ubiquitinated by ZNRF3, leading to its degradation by the proteasome.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Ubl conjugation

Proteomic databases

MaxQBiQ61090.
PRIDEiQ61090.

PTM databases

PhosphoSiteiQ61090.

Expressioni

Gene expression databases

CleanExiMM_FZD7.
ExpressionAtlasiQ61090. baseline and differential.
GenevestigatoriQ61090.

Interactioni

Subunit structurei

Interacts with MAGI3 and DVL1. Interacts with MYOC (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
Dlg4Q621084EBI-8473104,EBI-300895

Protein-protein interaction databases

IntActiQ61090. 4 interactions.
MINTiMINT-1780171.

Structurei

3D structure databases

ProteinModelPortaliQ61090.
SMRiQ61090. Positions 49-155, 228-563.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini44 – 163120FZPROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi550 – 5556Lys-Thr-X-X-X-Trp motif, mediates interaction with the PDZ domain of Dvl family membersBy similarity
Motifi570 – 5723PDZ-binding

Domaini

Lys-Thr-X-X-X-Trp motif interacts with the PDZ doman of Dvl (Disheveled) family members and is involved in the activation of the Wnt/beta-catenin signaling pathway.By similarity
The FZ domain is involved in binding with Wnt ligands.By similarity

Sequence similaritiesi

Contains 1 FZ (frizzled) domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG257258.
GeneTreeiENSGT00760000118864.
HOVERGENiHBG006977.
InParanoidiQ61090.
KOiK02432.
OMAiCVERFSE.
OrthoDBiEOG7M3J01.
TreeFamiTF317907.

Family and domain databases

Gene3Di1.10.2000.10. 1 hit.
InterProiIPR000539. Frizzled.
IPR015526. Frizzled/SFRP.
IPR020067. Frizzled_dom.
IPR026552. FZD7.
IPR017981. GPCR_2-like.
[Graphical view]
PANTHERiPTHR11309. PTHR11309. 1 hit.
PTHR11309:SF31. PTHR11309:SF31. 1 hit.
PfamiPF01534. Frizzled. 1 hit.
PF01392. Fz. 1 hit.
[Graphical view]
PRINTSiPR00489. FRIZZLED.
SMARTiSM00063. FRI. 1 hit.
[Graphical view]
SUPFAMiSSF63501. SSF63501. 1 hit.
PROSITEiPS50038. FZ. 1 hit.
PS50261. G_PROTEIN_RECEP_F2_4. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q61090-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRGPGTAASH SPLGLCALVL ALLGALPTDT RAQPYHGEKG ISVPDHGFCQ
60 70 80 90 100
PISIPLCTDI AYNQTILPNL LGHTNQEDAG LEVHQFYPLV KVQCSPELRF
110 120 130 140 150
FLCSMYAPVC TVLDQAIPPC RSLCERARQG CEALMNKFGF QWPERLRCEN
160 170 180 190 200
FPVHGAGEIC VGQNTSDGSG GAGGSPTAYP TAPYLPDPPF TAMSPSDGRG
210 220 230 240 250
RLSFPFSCPR QLKVPPYLGY RFLGERDCGA PCEPGRANGL MYFKEEERRF
260 270 280 290 300
ARLWVGVWSV LCCASTLFTV LTYLVDMRRF SYPERPIIFL SGCYFMVAVA
310 320 330 340 350
HVAGFLLEDR AVCVERFSDD GYRTVAQGTK KEGCTILFMV LYFFGMASSI
360 370 380 390 400
WWVILSLTWF LAAGMKWGHE AIEANSQYFH LAAWAVPAVK TITILAMGQV
410 420 430 440 450
DGDLLSGVCY VGLSSVDALR GFVLAPLFVY LFIGTSFLLA GFVSLFRIRT
460 470 480 490 500
IMKHDGTKTE KLEKLMVRIG VFSVLYTVPA TIVLACYFYE QAFREHWERT
510 520 530 540 550
WLLQTCKSYA VPCPPGHFSP MSPDFTVFMI KYLMTMIVGI TTGFWIWSGK
560 570
TLQSWRRFYH RLSHSSKGET AV
Length:572
Mass (Da):63,733
Last modified:October 3, 2012 - v2
Checksum:iD0B7DF37B9736183
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti262 – 2621C → S in AAC52432 (PubMed:8626800).Curated
Sequence conflicti516 – 5161G → R in AAC52432 (PubMed:8626800).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U43320 mRNA. Translation: AAC52432.1.
AC132574 Genomic DNA. No translation available.
CH466548 Genomic DNA. Translation: EDL00127.1.
CCDSiCCDS14985.1.
RefSeqiNP_032083.3. NM_008057.3.
UniGeneiMm.297906.

Genome annotation databases

EnsembliENSMUST00000114246; ENSMUSP00000109884; ENSMUSG00000041075.
GeneIDi14369.
KEGGimmu:14369.
UCSCiuc011wlq.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U43320 mRNA. Translation: AAC52432.1.
AC132574 Genomic DNA. No translation available.
CH466548 Genomic DNA. Translation: EDL00127.1.
CCDSiCCDS14985.1.
RefSeqiNP_032083.3. NM_008057.3.
UniGeneiMm.297906.

3D structure databases

ProteinModelPortaliQ61090.
SMRiQ61090. Positions 49-155, 228-563.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ61090. 4 interactions.
MINTiMINT-1780171.

Protein family/group databases

MEROPSiI93.001.
GPCRDBiSearch...

PTM databases

PhosphoSiteiQ61090.

Proteomic databases

MaxQBiQ61090.
PRIDEiQ61090.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000114246; ENSMUSP00000109884; ENSMUSG00000041075.
GeneIDi14369.
KEGGimmu:14369.
UCSCiuc011wlq.1. mouse.

Organism-specific databases

CTDi8324.
MGIiMGI:108570. Fzd7.

Phylogenomic databases

eggNOGiNOG257258.
GeneTreeiENSGT00760000118864.
HOVERGENiHBG006977.
InParanoidiQ61090.
KOiK02432.
OMAiCVERFSE.
OrthoDBiEOG7M3J01.
TreeFamiTF317907.

Enzyme and pathway databases

ReactomeiREACT_214043. PCP/CE pathway.
REACT_250061. Class B/2 (Secretin family receptors).

Miscellaneous databases

NextBioi285849.
PROiQ61090.
SOURCEiSearch...

Gene expression databases

CleanExiMM_FZD7.
ExpressionAtlasiQ61090. baseline and differential.
GenevestigatoriQ61090.

Family and domain databases

Gene3Di1.10.2000.10. 1 hit.
InterProiIPR000539. Frizzled.
IPR015526. Frizzled/SFRP.
IPR020067. Frizzled_dom.
IPR026552. FZD7.
IPR017981. GPCR_2-like.
[Graphical view]
PANTHERiPTHR11309. PTHR11309. 1 hit.
PTHR11309:SF31. PTHR11309:SF31. 1 hit.
PfamiPF01534. Frizzled. 1 hit.
PF01392. Fz. 1 hit.
[Graphical view]
PRINTSiPR00489. FRIZZLED.
SMARTiSM00063. FRI. 1 hit.
[Graphical view]
SUPFAMiSSF63501. SSF63501. 1 hit.
PROSITEiPS50038. FZ. 1 hit.
PS50261. G_PROTEIN_RECEP_F2_4. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "A large family of putative transmembrane receptors homologous to the product of the Drosophila tissue polarity gene frizzled."
    Wang Y., Macke J.P., Abella B.S., Andreasson K., Worley P., Gilbert D.J., Copeland N.G., Jenkins N.A., Nathans J.
    J. Biol. Chem. 271:4468-4476(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "Protein kinase C is differentially stimulated by Wnt and Frizzled homologs in a G-protein-dependent manner."
    Sheldahl L.C., Park M., Malbon C.C., Moon R.T.
    Curr. Biol. 9:695-698(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: COUPLING TO BETA-CATENIN PATHWAY.
  5. "Biochemical characterization of Wnt-frizzled interactions using a soluble, biologically active vertebrate Wnt protein."
    Hsieh J.C., Rattner A., Smallwood P.M., Nathans J.
    Proc. Natl. Acad. Sci. U.S.A. 96:3546-3551(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.
  6. "Characterization of function of three domains in dishevelled-1: DEP domain is responsible for membrane translocation of dishevelled-1."
    Pan W.J., Pang S.Z., Huang T., Guo H.Y., Wu D., Li L.
    Cell Res. 14:324-330(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH DVL1.
  7. "MAGI-3 is involved in the regulation of the JNK signaling pathway as a scaffold protein for frizzled and Ltap."
    Yao R., Natsume Y., Noda T.
    Oncogene 23:6023-6030(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH MAGI3.

Entry informationi

Entry nameiFZD7_MOUSE
AccessioniPrimary (citable) accession number: Q61090
Secondary accession number(s): G3X953
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 5, 2001
Last sequence update: October 3, 2012
Last modified: February 4, 2015
This is version 120 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.