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Protein

Laminin subunit beta-3

Gene

Lamb3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.

GO - Molecular functioni

GO - Biological processi

  • brown fat cell differentiation Source: MGI
  • cell adhesion Source: UniProtKB-KW
  • endodermal cell differentiation Source: Ensembl

Keywordsi

Biological processCell adhesion

Enzyme and pathway databases

ReactomeiR-MMU-1474228 Degradation of the extracellular matrix
R-MMU-2022090 Assembly of collagen fibrils and other multimeric structures
R-MMU-2214320 Anchoring fibril formation
R-MMU-3000157 Laminin interactions
R-MMU-446107 Type I hemidesmosome assembly
R-MMU-8874081 MET activates PTK2 signaling

Names & Taxonomyi

Protein namesi
Recommended name:
Laminin subunit beta-3
Alternative name(s):
Epiligrin subunit bata
Kalinin B1 chain
Kalinin subunit beta
Laminin-5 subunit beta
Nicein subunit beta
Gene namesi
Name:Lamb3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:99915 Lamb3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Basement membrane, Extracellular matrix, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 17Sequence analysisAdd BLAST17
ChainiPRO_000001707218 – 1168Laminin subunit beta-3Add BLAST1151

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi220N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi250 ↔ 259PROSITE-ProRule annotation
Disulfide bondi252 ↔ 276PROSITE-ProRule annotation
Disulfide bondi278 ↔ 287PROSITE-ProRule annotation
Disulfide bondi290 ↔ 310PROSITE-ProRule annotation
Disulfide bondi313 ↔ 322PROSITE-ProRule annotation
Disulfide bondi315 ↔ 340PROSITE-ProRule annotation
Disulfide bondi343 ↔ 352PROSITE-ProRule annotation
Disulfide bondi355 ↔ 373PROSITE-ProRule annotation
Disulfide bondi376 ↔ 389PROSITE-ProRule annotation
Disulfide bondi378 ↔ 396PROSITE-ProRule annotation
Disulfide bondi398 ↔ 407PROSITE-ProRule annotation
Disulfide bondi410 ↔ 425PROSITE-ProRule annotation
Disulfide bondi428 ↔ 441PROSITE-ProRule annotation
Disulfide bondi430 ↔ 448PROSITE-ProRule annotation
Disulfide bondi450 ↔ 459PROSITE-ProRule annotation
Disulfide bondi462 ↔ 475PROSITE-ProRule annotation
Disulfide bondi478 ↔ 490PROSITE-ProRule annotation
Disulfide bondi480 ↔ 497PROSITE-ProRule annotation
Disulfide bondi499 ↔ 508PROSITE-ProRule annotation
Disulfide bondi516 ↔ 528PROSITE-ProRule annotation
Disulfide bondi531 ↔ 543PROSITE-ProRule annotation
Disulfide bondi533 ↔ 550PROSITE-ProRule annotation
Disulfide bondi552 ↔ 561PROSITE-ProRule annotation
Disulfide bondi564 ↔ 575PROSITE-ProRule annotation
Disulfide bondi578InterchainCurated
Disulfide bondi581InterchainCurated
Glycosylationi601N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi806N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi1167InterchainCurated

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiQ61087
PaxDbiQ61087
PeptideAtlasiQ61087
PRIDEiQ61087

PTM databases

iPTMnetiQ61087
PhosphoSitePlusiQ61087

Expressioni

Tissue specificityi

Found in the basement membranes (major component).

Gene expression databases

BgeeiENSMUSG00000026639
CleanExiMM_LAMB3
ExpressionAtlasiQ61087 baseline and differential
GenevisibleiQ61087 MM

Interactioni

Subunit structurei

Laminin is a complex glycoprotein, consisting of three different polypeptide chains (alpha, beta, gamma), which are bound to each other by disulfide bonds into a cross-shaped molecule comprising one long and three short arms with globules at each end. Beta-3 is a subunit of laminin-5 (laminin-332 or epiligrin/kalinin/nicein). Interacts with ECM1 (By similarity).By similarity

Protein-protein interaction databases

IntActiQ61087, 1 interactor
MINTiQ61087
STRINGi10090.ENSMUSP00000016315

Structurei

3D structure databases

ProteinModelPortaliQ61087
SMRiQ61087
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini22 – 249Laminin N-terminalPROSITE-ProRule annotationAdd BLAST228
Domaini250 – 312Laminin EGF-like 1PROSITE-ProRule annotationAdd BLAST63
Domaini313 – 375Laminin EGF-like 2PROSITE-ProRule annotationAdd BLAST63
Domaini376 – 427Laminin EGF-like 3PROSITE-ProRule annotationAdd BLAST52
Domaini428 – 477Laminin EGF-like 4PROSITE-ProRule annotationAdd BLAST50
Domaini478 – 530Laminin EGF-like 5PROSITE-ProRule annotationAdd BLAST53
Domaini531 – 577Laminin EGF-like 6PROSITE-ProRule annotationAdd BLAST47

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni576 – 781Domain IIAdd BLAST206
Regioni782 – 812Domain alphaAdd BLAST31
Regioni813 – 1168Domain IAdd BLAST356

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili732 – 754Sequence analysisAdd BLAST23
Coiled coili827 – 879Sequence analysisAdd BLAST53
Coiled coili944 – 1129Sequence analysisAdd BLAST186

Domaini

The alpha-helical domains I and II are thought to interact with other laminin chains to form a coiled coil structure.
Domain VI is globular.

Keywords - Domaini

Coiled coil, Laminin EGF-like domain, Repeat, Signal

Phylogenomic databases

eggNOGiKOG0994 Eukaryota
ENOG410XPEG LUCA
GeneTreeiENSGT00780000121851
HOGENOMiHOG000113279
HOVERGENiHBG052302
InParanoidiQ61087
KOiK06244
OMAiWWQSQND
OrthoDBiEOG091G019I
TreeFamiTF352481

Family and domain databases

Gene3Di2.60.120.1490, 1 hit
InterProiView protein in InterPro
IPR000742 EGF-like_dom
IPR002049 Laminin_EGF
IPR008211 Laminin_N
IPR038684 Laminin_N_sf
PfamiView protein in Pfam
PF00053 Laminin_EGF, 6 hits
PF00055 Laminin_N, 1 hit
SMARTiView protein in SMART
SM00181 EGF, 4 hits
SM00180 EGF_Lam, 6 hits
SM00136 LamNT, 1 hit
PROSITEiView protein in PROSITE
PS00022 EGF_1, 5 hits
PS01186 EGF_2, 2 hits
PS01248 EGF_LAM_1, 4 hits
PS50027 EGF_LAM_2, 6 hits
PS51117 LAMININ_NTER, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q61087-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTAFFLLWLA LPGFLCAQQA CSRGACYPPV GDLLIGRTQL LRASSTCGLT
60 70 80 90 100
KPETYCTQYG QWQMKCCKCD SRLPRNYNSH RVENVASSSG PMRWWQSQND
110 120 130 140 150
VSPVSLQLDL DKRMQLQDIM MDFKGLTPAG MLIERSSDFG KTWRVYQYLA
160 170 180 190 200
TDCASTFPQV HQGQPKNWQD VRCRPLSQRP NGHLTGGKVQ LNLMDLASAI
210 220 230 240 250
PASQSKKIQE LGDITNLRVN FTKLAPVPQR GSYPPSAYFA VSQLRLQGSC
260 270 280 290 300
FCHGHADRCA PNPGGSTTAV QVNNVCVCQH NTAGPNCDRC APFYNNRPWR
310 320 330 340 350
PAEGQDTHEC QRCDCNGHSE TCHFDPAVFA ASQGTNGGVC DNCRDHTEGK
360 370 380 390 400
NCERCQLHYF RNRRPSAPIH ETCIPCECDP DGAVQGAPCD RLTGQCVCKE
410 420 430 440 450
YVQGERCDLC KPGFTGLTFA NPKGCHACDC SILGARKDMP CEEETGRCLC
460 470 480 490 500
LPNVVGPKCD QCAPSHWKLA SGLGCEPCAC DPRNSLSSQC NQFTGQCPCR
510 520 530 540 550
EGFGGLTCSS AAIRQCPDQT YGHVATGCRA CDCDFRGTEG PGCDKASGRC
560 570 580 590 600
LCRPGFTGPR CDQCQRGHCD RYPVCVACHS CFQAYDTDLQ EQARRLHSLR
610 620 630 640 650
NATEGLWTGT GLEDHGLASR LLDAKSKIEQ IRQILEGTSV TEQDVAQVAN
660 670 680 690 700
GILSIRRTLQ GLPLDLPLEE EMESFSGDLG NLDRSFSRLL LMYRSKKEQF
710 720 730 740 750
EKLSSEDPSG AFRMLTMAYE QSSRAAQQVS DSSSLLSQLR DSRREAEGLE
760 770 780 790 800
RQAGGGGTGG AQLMALRLEM ASLPDLTPTI NKLCGRSRQT ACTPGDCPGE
810 820 830 840 850
LCPQDNGTAC GSHCRGALPR AKGAFHMAGR VAEQLRNFNT QLQQTRQMIR
860 870 880 890 900
AAEEAASRVQ ADAQRLETQV STSRLLMEED VQRTRLLIQQ VRGFLTDPDT
910 920 930 940 950
DAATIQQVSE AVLALWLPTD SATVLRKMKE IQAIAARLPN VDSVLSQTKQ
960 970 980 990 1000
DIARARRLQA EAEQARSRAH AVEGQVDDVV GNLRQGTVAL QEAQDTMQGT
1010 1020 1030 1040 1050
GRSLRLIQER VGEVQQVLVP AERLVKGMKE QMSGFWARMK ELRRQAQEEQ
1060 1070 1080 1090 1100
AQAMQARQLA EGASQQAMNA QEGFKRLKQK YTELKDRLGQ SPVLGEQGNR
1110 1120 1130 1140 1150
ILSIKMEAEE LFGETMEMMD KMKDMESELL RGSQAIMLRS ADLSGLEKRV
1160
EQIRSYINGR VLYYATCK
Length:1,168
Mass (Da):128,900
Last modified:July 27, 2011 - v2
Checksum:i66EB2BE1AA232215
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti34L → F in AAA85255 (PubMed:7898049).Curated1
Sequence conflicti271Q → R in AAA85255 (PubMed:7898049).Curated1
Sequence conflicti284G → A in AAA85255 (PubMed:7898049).Curated1
Sequence conflicti320E → L in AAA85255 (PubMed:7898049).Curated1
Sequence conflicti354R → P in AAA85255 (PubMed:7898049).Curated1
Sequence conflicti361R → W in AAA85255 (PubMed:7898049).Curated1
Sequence conflicti420A → D in AAA85255 (PubMed:7898049).Curated1
Sequence conflicti525A → P in AAA85255 (PubMed:7898049).Curated1
Sequence conflicti542G → A in AAA85255 (PubMed:7898049).Curated1
Sequence conflicti646A → P in AAA85255 (PubMed:7898049).Curated1
Sequence conflicti1079Q → P in AAA85255 (PubMed:7898049).Curated1
Sequence conflicti1115T → S in AAA85255 (PubMed:7898049).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U43298 mRNA Translation: AAA85255.1
AK155769 mRNA Translation: BAE33428.1
CH466555 Genomic DNA Translation: EDL12945.1
CH466555 Genomic DNA Translation: EDL12946.1
BC008516 mRNA Translation: AAH08516.1
CCDSiCCDS15637.1
RefSeqiNP_001264857.1, NM_001277928.1
NP_032510.2, NM_008484.2
XP_006497295.1, XM_006497232.1
XP_006497296.1, XM_006497233.2
UniGeneiMm.435441

Genome annotation databases

EnsembliENSMUST00000016315; ENSMUSP00000016315; ENSMUSG00000026639
ENSMUST00000159955; ENSMUSP00000123875; ENSMUSG00000026639
ENSMUST00000194677; ENSMUSP00000142053; ENSMUSG00000026639
GeneIDi16780
KEGGimmu:16780
UCSCiuc007eee.1 mouse

Similar proteinsi

Entry informationi

Entry nameiLAMB3_MOUSE
AccessioniPrimary (citable) accession number: Q61087
Secondary accession number(s): Q91V90
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: July 27, 2011
Last modified: May 23, 2018
This is version 153 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

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