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Q61084

- M3K3_MOUSE

UniProt

Q61084 - M3K3_MOUSE

Protein

Mitogen-activated protein kinase kinase kinase 3

Gene

Map3k3

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 129 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Component of a protein kinase signal transduction cascade. Mediates activation of the NF-kappa-B, AP1 and DDIT3 transcriptional regulators.1 Publication

    Catalytic activityi

    ATP + a protein = ADP + a phosphoprotein.

    Cofactori

    Magnesium.

    Enzyme regulationi

    Activated by phosphorylation on Thr-530.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei391 – 3911ATPPROSITE-ProRule annotation
    Active sitei489 – 4891Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi368 – 3769ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. MAP kinase kinase kinase activity Source: UniProtKB-EC
    3. metal ion binding Source: UniProtKB-KW
    4. protein binding Source: IntAct
    5. protein kinase activity Source: MGI

    GO - Biological processi

    1. intracellular signal transduction Source: MGI
    2. positive regulation of I-kappaB kinase/NF-kappaB signaling Source: Ensembl
    3. protein autophosphorylation Source: MGI

    Keywords - Molecular functioni

    Kinase, Serine/threonine-protein kinase, Transferase

    Keywords - Ligandi

    ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    BRENDAi2.7.12.2. 3474.
    ReactomeiREACT_224208. Interleukin-1 signaling.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Mitogen-activated protein kinase kinase kinase 3 (EC:2.7.11.25)
    Alternative name(s):
    MAPK/ERK kinase kinase 3
    Short name:
    MEK kinase 3
    Short name:
    MEKK 3
    Gene namesi
    Name:Map3k3
    Synonyms:Mekk3
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 11

    Organism-specific databases

    MGIiMGI:1346874. Map3k3.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 626626Mitogen-activated protein kinase kinase kinase 3PRO_0000086246Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei166 – 1661Phosphoserine; by SGK1
    Modified residuei250 – 2501PhosphoserineBy similarity
    Modified residuei337 – 3371Phosphoserine; by SGK11 Publication
    Modified residuei340 – 3401Phosphoserine1 Publication

    Post-translational modificationi

    Phosphorylation at Ser-166 and Ser-337 by SGK1 inhibits its activity.By similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiQ61084.
    PRIDEiQ61084.

    PTM databases

    PhosphoSiteiQ61084.

    Expressioni

    Gene expression databases

    BgeeiQ61084.
    CleanExiMM_MAP3K3.
    GenevestigatoriQ61084.

    Interactioni

    Subunit structurei

    Binds both upstream activators and downstream substrates in multimolecular complexes. Part of a complex with MAP2K3, RAC1 and CCM2. Interacts with MAP2K5 and SPAG9.2 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    Map2k5Q9WVS715EBI-446250,EBI-446144
    Traf6P701965EBI-446250,EBI-448028

    Protein-protein interaction databases

    BioGridi204959. 1 interaction.
    IntActiQ61084. 4 interactions.
    MINTiMINT-1531397.

    Structurei

    3D structure databases

    ProteinModelPortaliQ61084.
    SMRiQ61084. Positions 42-126, 336-624.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini44 – 12380OPRAdd
    BLAST
    Domaini362 – 622261Protein kinasePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 OPR domain.Curated
    Contains 1 protein kinase domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0515.
    GeneTreeiENSGT00750000117243.
    HOGENOMiHOG000049130.
    HOVERGENiHBG006303.
    InParanoidiQ61084.
    KOiK04421.
    OMAiQALHSIM.
    OrthoDBiEOG747PHJ.
    PhylomeDBiQ61084.
    TreeFamiTF105113.

    Family and domain databases

    InterProiIPR011009. Kinase-like_dom.
    IPR000270. OPR_PB1.
    IPR000719. Prot_kinase_dom.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    [Graphical view]
    PfamiPF00564. PB1. 1 hit.
    PF00069. Pkinase. 1 hit.
    [Graphical view]
    SMARTiSM00666. PB1. 1 hit.
    SM00220. S_TKc. 1 hit.
    [Graphical view]
    SUPFAMiSSF56112. SSF56112. 1 hit.
    PROSITEiPS50011. PROTEIN_KINASE_DOM. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q61084-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDEQEALDSI MKDLVALQMS RRTRLSGYET MKNKDTGHPN RQSDVRIKFE    50
    HNGERRIIAF SRPVRYEDVE HKVTTVFGQP LDLHYMNNEL SILLKNQDDL 100
    DKAIDILDRS SSMKSLRILL LSQDRNHTSS SPHSGVSRQV RIKPSQSAGD 150
    INTIYQAPEP RSRHLSVSSQ NPGRSSPPPG YVPERQQHIA RQGSYTSINS 200
    EGEFIPETSE QCMLDPLSSA ENSLSGSCQS LDRSADSPSF RKSQMSRARS 250
    FPDNRKECSD RETQLYDKGV KGGTYPRRYH VSVHHKDYND GRRTFPRIRR 300
    HQGNLFTLVP SSRSLSTNGE NMGVAVQYLD PRGRLRSADS ENALTVQERN 350
    VPTKSPSAPI NWRRGKLLGQ GAFGRVYLCY DVDTGRELAS KQVQFDPDSP 400
    ETSKEVSALE CEIQLLKNLQ HERIVQYYGC LRDRAEKILT IFMEYMPGGS 450
    VKDQLKAYGA LTESVTRKYT RQILEGMSYL HSNMIVHRDI KGANILRDSA 500
    GNVKLGDFGA SKRLQTICMS GTGIRSVTGT PYWMSPEVIS GEGYGRKADV 550
    WSLGCTVVEM LTEKPPWAEY EAMAAIFKIA TQPTNPQLPS HISEHGRDFL 600
    RRIFVEARQR PSAEELLTHH FAQLVY 626
    Length:626
    Mass (Da):70,776
    Last modified:November 1, 1996 - v1
    Checksum:i00EF2442C9E56E0B
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U43187 mRNA. Translation: AAB03535.1.
    BC023781 mRNA. Translation: AAH23781.1.
    CCDSiCCDS25548.1.
    RefSeqiNP_036077.1. NM_011947.3.
    UniGeneiMm.27041.

    Genome annotation databases

    EnsembliENSMUST00000002044; ENSMUSP00000002044; ENSMUSG00000020700.
    GeneIDi26406.
    KEGGimmu:26406.
    UCSCiuc007lyc.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U43187 mRNA. Translation: AAB03535.1 .
    BC023781 mRNA. Translation: AAH23781.1 .
    CCDSi CCDS25548.1.
    RefSeqi NP_036077.1. NM_011947.3.
    UniGenei Mm.27041.

    3D structure databases

    ProteinModelPortali Q61084.
    SMRi Q61084. Positions 42-126, 336-624.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 204959. 1 interaction.
    IntActi Q61084. 4 interactions.
    MINTi MINT-1531397.

    PTM databases

    PhosphoSitei Q61084.

    Proteomic databases

    PaxDbi Q61084.
    PRIDEi Q61084.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000002044 ; ENSMUSP00000002044 ; ENSMUSG00000020700 .
    GeneIDi 26406.
    KEGGi mmu:26406.
    UCSCi uc007lyc.1. mouse.

    Organism-specific databases

    CTDi 4215.
    MGIi MGI:1346874. Map3k3.

    Phylogenomic databases

    eggNOGi COG0515.
    GeneTreei ENSGT00750000117243.
    HOGENOMi HOG000049130.
    HOVERGENi HBG006303.
    InParanoidi Q61084.
    KOi K04421.
    OMAi QALHSIM.
    OrthoDBi EOG747PHJ.
    PhylomeDBi Q61084.
    TreeFami TF105113.

    Enzyme and pathway databases

    BRENDAi 2.7.12.2. 3474.
    Reactomei REACT_224208. Interleukin-1 signaling.

    Miscellaneous databases

    ChiTaRSi MAP3K3. mouse.
    NextBioi 304381.
    PROi Q61084.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q61084.
    CleanExi MM_MAP3K3.
    Genevestigatori Q61084.

    Family and domain databases

    InterProi IPR011009. Kinase-like_dom.
    IPR000270. OPR_PB1.
    IPR000719. Prot_kinase_dom.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    [Graphical view ]
    Pfami PF00564. PB1. 1 hit.
    PF00069. Pkinase. 1 hit.
    [Graphical view ]
    SMARTi SM00666. PB1. 1 hit.
    SM00220. S_TKc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56112. SSF56112. 1 hit.
    PROSITEi PS50011. PROTEIN_KINASE_DOM. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning of mitogen-activated protein/ERK kinase kinases (MEKK) 2 and 3. Regulation of sequential phosphorylation pathways involving mitogen-activated protein kinase and c-Jun kinase."
      Blank J.L., Gerwins P., Elliott E.M., Sather S., Johnson G.L.
      J. Biol. Chem. 271:5361-5368(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION.
      Tissue: Brain.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: FVB/N.
      Tissue: Mammary gland.
    3. "JLP: a scaffolding protein that tethers JNK/p38MAPK signaling modules and transcription factors."
      Lee C.M., Onesime D., Reddy C.D., Dhanasekaran N., Reddy E.P.
      Proc. Natl. Acad. Sci. U.S.A. 99:14189-14194(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH SPAG9.
    4. Cited for: INTERACTION WITH MAP2K3; RAC1 AND CCM2.
    5. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-337 AND SER-340, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Liver.

    Entry informationi

    Entry nameiM3K3_MOUSE
    AccessioniPrimary (citable) accession number: Q61084
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 129 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. Human and mouse protein kinases
      Human and mouse protein kinases: classification and index
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3