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Q61084 (M3K3_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 127. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Mitogen-activated protein kinase kinase kinase 3

EC=2.7.11.25
Alternative name(s):
MAPK/ERK kinase kinase 3
Short name=MEK kinase 3
Short name=MEKK 3
Gene names
Name:Map3k3
Synonyms:Mekk3
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length626 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Component of a protein kinase signal transduction cascade. Mediates activation of the NF-kappa-B, AP1 and DDIT3 transcriptional regulators. Ref.1

Catalytic activity

ATP + a protein = ADP + a phosphoprotein.

Cofactor

Magnesium.

Enzyme regulation

Activated by phosphorylation on Thr-530 By similarity.

Subunit structure

Binds both upstream activators and downstream substrates in multimolecular complexes. Part of a complex with MAP2K3, RAC1 and CCM2. Interacts with MAP2K5 and SPAG9. Ref.3 Ref.4

Post-translational modification

Phosphorylation at Ser-166 and Ser-337 by SGK1 inhibits its activity By similarity.

Sequence similarities

Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.

Contains 1 OPR domain.

Contains 1 protein kinase domain.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Map2k5Q9WVS715EBI-446250,EBI-446144
Traf6P701965EBI-446250,EBI-448028

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 626626Mitogen-activated protein kinase kinase kinase 3
PRO_0000086246

Regions

Domain44 – 12380OPR
Domain362 – 622261Protein kinase
Nucleotide binding368 – 3769ATP By similarity

Sites

Active site4891Proton acceptor By similarity
Binding site3911ATP By similarity

Amino acid modifications

Modified residue1661Phosphoserine; by SGK1
Modified residue2501Phosphoserine By similarity
Modified residue3371Phosphoserine; by SGK1 Ref.5
Modified residue3401Phosphoserine Ref.5

Sequences

Sequence LengthMass (Da)Tools
Q61084 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 00EF2442C9E56E0B

FASTA62670,776
        10         20         30         40         50         60 
MDEQEALDSI MKDLVALQMS RRTRLSGYET MKNKDTGHPN RQSDVRIKFE HNGERRIIAF 

        70         80         90        100        110        120 
SRPVRYEDVE HKVTTVFGQP LDLHYMNNEL SILLKNQDDL DKAIDILDRS SSMKSLRILL 

       130        140        150        160        170        180 
LSQDRNHTSS SPHSGVSRQV RIKPSQSAGD INTIYQAPEP RSRHLSVSSQ NPGRSSPPPG 

       190        200        210        220        230        240 
YVPERQQHIA RQGSYTSINS EGEFIPETSE QCMLDPLSSA ENSLSGSCQS LDRSADSPSF 

       250        260        270        280        290        300 
RKSQMSRARS FPDNRKECSD RETQLYDKGV KGGTYPRRYH VSVHHKDYND GRRTFPRIRR 

       310        320        330        340        350        360 
HQGNLFTLVP SSRSLSTNGE NMGVAVQYLD PRGRLRSADS ENALTVQERN VPTKSPSAPI 

       370        380        390        400        410        420 
NWRRGKLLGQ GAFGRVYLCY DVDTGRELAS KQVQFDPDSP ETSKEVSALE CEIQLLKNLQ 

       430        440        450        460        470        480 
HERIVQYYGC LRDRAEKILT IFMEYMPGGS VKDQLKAYGA LTESVTRKYT RQILEGMSYL 

       490        500        510        520        530        540 
HSNMIVHRDI KGANILRDSA GNVKLGDFGA SKRLQTICMS GTGIRSVTGT PYWMSPEVIS 

       550        560        570        580        590        600 
GEGYGRKADV WSLGCTVVEM LTEKPPWAEY EAMAAIFKIA TQPTNPQLPS HISEHGRDFL 

       610        620 
RRIFVEARQR PSAEELLTHH FAQLVY 

« Hide

References

« Hide 'large scale' references
[1]"Molecular cloning of mitogen-activated protein/ERK kinase kinases (MEKK) 2 and 3. Regulation of sequential phosphorylation pathways involving mitogen-activated protein kinase and c-Jun kinase."
Blank J.L., Gerwins P., Elliott E.M., Sather S., Johnson G.L.
J. Biol. Chem. 271:5361-5368(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION.
Tissue: Brain.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: FVB/N.
Tissue: Mammary gland.
[3]"JLP: a scaffolding protein that tethers JNK/p38MAPK signaling modules and transcription factors."
Lee C.M., Onesime D., Reddy C.D., Dhanasekaran N., Reddy E.P.
Proc. Natl. Acad. Sci. U.S.A. 99:14189-14194(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH SPAG9.
[4]"Rac-MEKK3-MKK3 scaffolding for p38 MAPK activation during hyperosmotic shock."
Uhlik M.T., Abell A.N., Johnson N.L., Sun W., Cuevas B.D., Lobel-Rice K.E., Horne E.A., Dell'Acqua M.L., Johnson G.L.
Nat. Cell Biol. 5:1104-1110(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH MAP2K3; RAC1 AND CCM2.
[5]"Large-scale phosphorylation analysis of mouse liver."
Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-337 AND SER-340, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Liver.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U43187 mRNA. Translation: AAB03535.1.
BC023781 mRNA. Translation: AAH23781.1.
CCDSCCDS25548.1.
RefSeqNP_036077.1. NM_011947.3.
UniGeneMm.27041.

3D structure databases

ProteinModelPortalQ61084.
SMRQ61084. Positions 42-126, 336-624.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid204959. 1 interaction.
IntActQ61084. 4 interactions.
MINTMINT-1531397.

PTM databases

PhosphoSiteQ61084.

Proteomic databases

PaxDbQ61084.
PRIDEQ61084.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000002044; ENSMUSP00000002044; ENSMUSG00000020700.
GeneID26406.
KEGGmmu:26406.
UCSCuc007lyc.1. mouse.

Organism-specific databases

CTD4215.
MGIMGI:1346874. Map3k3.

Phylogenomic databases

eggNOGCOG0515.
GeneTreeENSGT00750000117243.
HOGENOMHOG000049130.
HOVERGENHBG006303.
InParanoidQ61084.
KOK04421.
OMAQALHSIM.
OrthoDBEOG747PHJ.
PhylomeDBQ61084.
TreeFamTF105113.

Enzyme and pathway databases

BRENDA2.7.12.2. 3474.

Gene expression databases

BgeeQ61084.
CleanExMM_MAP3K3.
GenevestigatorQ61084.

Family and domain databases

InterProIPR011009. Kinase-like_dom.
IPR000270. OPR_PB1.
IPR000719. Prot_kinase_dom.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
[Graphical view]
PfamPF00564. PB1. 1 hit.
PF00069. Pkinase. 1 hit.
[Graphical view]
SMARTSM00666. PB1. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMSSF56112. SSF56112. 1 hit.
PROSITEPS50011. PROTEIN_KINASE_DOM. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSMAP3K3. mouse.
NextBio304381.
PROQ61084.
SOURCESearch...

Entry information

Entry nameM3K3_MOUSE
AccessionPrimary (citable) accession number: Q61084
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: July 9, 2014
This is version 127 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Human and mouse protein kinases

Human and mouse protein kinases: classification and index

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot