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Protein

Mitogen-activated protein kinase kinase kinase 3

Gene

Map3k3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of a protein kinase signal transduction cascade. Mediates activation of the NF-kappa-B, AP1 and DDIT3 transcriptional regulators.1 Publication

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Cofactori

Enzyme regulationi

Activated by phosphorylation on Thr-530.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei391ATPPROSITE-ProRule annotation1
Active sitei489Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi368 – 376ATPPROSITE-ProRule annotation9

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Serine/threonine-protein kinase, Transferase
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.12.2 3474
ReactomeiR-MMU-9020702 Interleukin-1 signaling

Names & Taxonomyi

Protein namesi
Recommended name:
Mitogen-activated protein kinase kinase kinase 3 (EC:2.7.11.25)
Alternative name(s):
MAPK/ERK kinase kinase 3
Short name:
MEK kinase 3
Short name:
MEKK 3
Gene namesi
Name:Map3k3
Synonyms:Mekk3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:1346874 Map3k3

Subcellular locationi

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000862461 – 626Mitogen-activated protein kinase kinase kinase 3Add BLAST626

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei147PhosphoserineCombined sources1
Modified residuei166PhosphoserineCombined sources1
Modified residuei250PhosphoserineBy similarity1
Modified residuei312PhosphoserineBy similarity1
Modified residuei337Phosphoserine; by SGK1Combined sources1
Modified residuei340PhosphoserineCombined sources1

Post-translational modificationi

Phosphorylation at Ser-166 and Ser-337 by SGK1 inhibits its activity.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ61084
MaxQBiQ61084
PaxDbiQ61084
PRIDEiQ61084

PTM databases

iPTMnetiQ61084
PhosphoSitePlusiQ61084

Expressioni

Gene expression databases

BgeeiENSMUSG00000020700
CleanExiMM_MAP3K3
GenevisibleiQ61084 MM

Interactioni

Subunit structurei

Binds both upstream activators and downstream substrates in multimolecular complexes. Part of a complex with MAP2K3, RAC1 and CCM2. Interacts with MAP2K5 and SPAG9.2 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi204959, 1 interactor
CORUMiQ61084
IntActiQ61084, 4 interactors
MINTiQ61084
STRINGi10090.ENSMUSP00000002044

Structurei

3D structure databases

ProteinModelPortaliQ61084
SMRiQ61084
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini44 – 123PB1PROSITE-ProRule annotationAdd BLAST80
Domaini362 – 622Protein kinasePROSITE-ProRule annotationAdd BLAST261

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0198 Eukaryota
ENOG410XQGS LUCA
GeneTreeiENSGT00800000124036
HOGENOMiHOG000049130
HOVERGENiHBG006303
InParanoidiQ61084
KOiK04421
OMAiRIRRPQG
OrthoDBiEOG091G038L
PhylomeDBiQ61084
TreeFamiTF105113

Family and domain databases

CDDicd06405 PB1_Mekk2_3, 1 hit
InterProiView protein in InterPro
IPR011009 Kinase-like_dom_sf
IPR000270 PB1_dom
IPR034879 PB1_MEKK2/3
IPR000719 Prot_kinase_dom
PfamiView protein in Pfam
PF00564 PB1, 1 hit
PF00069 Pkinase, 1 hit
SMARTiView protein in SMART
SM00666 PB1, 1 hit
SM00220 S_TKc, 1 hit
SUPFAMiSSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS51745 PB1, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit

Sequencei

Sequence statusi: Complete.

Q61084-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDEQEALDSI MKDLVALQMS RRTRLSGYET MKNKDTGHPN RQSDVRIKFE
60 70 80 90 100
HNGERRIIAF SRPVRYEDVE HKVTTVFGQP LDLHYMNNEL SILLKNQDDL
110 120 130 140 150
DKAIDILDRS SSMKSLRILL LSQDRNHTSS SPHSGVSRQV RIKPSQSAGD
160 170 180 190 200
INTIYQAPEP RSRHLSVSSQ NPGRSSPPPG YVPERQQHIA RQGSYTSINS
210 220 230 240 250
EGEFIPETSE QCMLDPLSSA ENSLSGSCQS LDRSADSPSF RKSQMSRARS
260 270 280 290 300
FPDNRKECSD RETQLYDKGV KGGTYPRRYH VSVHHKDYND GRRTFPRIRR
310 320 330 340 350
HQGNLFTLVP SSRSLSTNGE NMGVAVQYLD PRGRLRSADS ENALTVQERN
360 370 380 390 400
VPTKSPSAPI NWRRGKLLGQ GAFGRVYLCY DVDTGRELAS KQVQFDPDSP
410 420 430 440 450
ETSKEVSALE CEIQLLKNLQ HERIVQYYGC LRDRAEKILT IFMEYMPGGS
460 470 480 490 500
VKDQLKAYGA LTESVTRKYT RQILEGMSYL HSNMIVHRDI KGANILRDSA
510 520 530 540 550
GNVKLGDFGA SKRLQTICMS GTGIRSVTGT PYWMSPEVIS GEGYGRKADV
560 570 580 590 600
WSLGCTVVEM LTEKPPWAEY EAMAAIFKIA TQPTNPQLPS HISEHGRDFL
610 620
RRIFVEARQR PSAEELLTHH FAQLVY
Length:626
Mass (Da):70,776
Last modified:November 1, 1996 - v1
Checksum:i00EF2442C9E56E0B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U43187 mRNA Translation: AAB03535.1
BC023781 mRNA Translation: AAH23781.1
CCDSiCCDS25548.1
RefSeqiNP_036077.1, NM_011947.3
UniGeneiMm.27041

Genome annotation databases

EnsembliENSMUST00000002044; ENSMUSP00000002044; ENSMUSG00000020700
GeneIDi26406
KEGGimmu:26406
UCSCiuc007lyc.1 mouse

Similar proteinsi

Entry informationi

Entry nameiM3K3_MOUSE
AccessioniPrimary (citable) accession number: Q61084
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: May 23, 2018
This is version 161 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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