SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q61084

- M3K3_MOUSE

UniProt

Q61084 - M3K3_MOUSE

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein
Mitogen-activated protein kinase kinase kinase 3
Gene
Map3k3, Mekk3
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Component of a protein kinase signal transduction cascade. Mediates activation of the NF-kappa-B, AP1 and DDIT3 transcriptional regulators.1 Publication

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Cofactori

Magnesium.

Enzyme regulationi

Activated by phosphorylation on Thr-530 By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei391 – 3911ATP By similarity
Active sitei489 – 4891Proton acceptor By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi368 – 3769ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. MAP kinase kinase kinase activity Source: UniProtKB-EC
  3. metal ion binding Source: UniProtKB-KW
  4. protein binding Source: IntAct
  5. protein kinase activity Source: MGI

GO - Biological processi

  1. intracellular signal transduction Source: MGI
  2. positive regulation of I-kappaB kinase/NF-kappaB signaling Source: Ensembl
  3. protein autophosphorylation Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.12.2. 3474.
ReactomeiREACT_224208. Interleukin-1 signaling.

Names & Taxonomyi

Protein namesi
Recommended name:
Mitogen-activated protein kinase kinase kinase 3 (EC:2.7.11.25)
Alternative name(s):
MAPK/ERK kinase kinase 3
Short name:
MEK kinase 3
Short name:
MEKK 3
Gene namesi
Name:Map3k3
Synonyms:Mekk3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 11

Organism-specific databases

MGIiMGI:1346874. Map3k3.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 626626Mitogen-activated protein kinase kinase kinase 3
PRO_0000086246Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei166 – 1661Phosphoserine; by SGK1
Modified residuei250 – 2501Phosphoserine By similarity
Modified residuei337 – 3371Phosphoserine; by SGK11 Publication
Modified residuei340 – 3401Phosphoserine1 Publication

Post-translational modificationi

Phosphorylation at Ser-166 and Ser-337 by SGK1 inhibits its activity By similarity.

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ61084.
PRIDEiQ61084.

PTM databases

PhosphoSiteiQ61084.

Expressioni

Gene expression databases

BgeeiQ61084.
CleanExiMM_MAP3K3.
GenevestigatoriQ61084.

Interactioni

Subunit structurei

Binds both upstream activators and downstream substrates in multimolecular complexes. Part of a complex with MAP2K3, RAC1 and CCM2. Interacts with MAP2K5 and SPAG9.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Map2k5Q9WVS715EBI-446250,EBI-446144
Traf6P701965EBI-446250,EBI-448028

Protein-protein interaction databases

BioGridi204959. 1 interaction.
IntActiQ61084. 4 interactions.
MINTiMINT-1531397.

Structurei

3D structure databases

ProteinModelPortaliQ61084.
SMRiQ61084. Positions 42-126, 336-624.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini44 – 12380OPR
Add
BLAST
Domaini362 – 622261Protein kinase
Add
BLAST

Sequence similaritiesi

Contains 1 OPR domain.

Phylogenomic databases

eggNOGiCOG0515.
GeneTreeiENSGT00750000117243.
HOGENOMiHOG000049130.
HOVERGENiHBG006303.
InParanoidiQ61084.
KOiK04421.
OMAiQALHSIM.
OrthoDBiEOG747PHJ.
PhylomeDBiQ61084.
TreeFamiTF105113.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000270. OPR_PB1.
IPR000719. Prot_kinase_dom.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
[Graphical view]
PfamiPF00564. PB1. 1 hit.
PF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00666. PB1. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS50011. PROTEIN_KINASE_DOM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q61084-1 [UniParc]FASTAAdd to Basket

« Hide

MDEQEALDSI MKDLVALQMS RRTRLSGYET MKNKDTGHPN RQSDVRIKFE    50
HNGERRIIAF SRPVRYEDVE HKVTTVFGQP LDLHYMNNEL SILLKNQDDL 100
DKAIDILDRS SSMKSLRILL LSQDRNHTSS SPHSGVSRQV RIKPSQSAGD 150
INTIYQAPEP RSRHLSVSSQ NPGRSSPPPG YVPERQQHIA RQGSYTSINS 200
EGEFIPETSE QCMLDPLSSA ENSLSGSCQS LDRSADSPSF RKSQMSRARS 250
FPDNRKECSD RETQLYDKGV KGGTYPRRYH VSVHHKDYND GRRTFPRIRR 300
HQGNLFTLVP SSRSLSTNGE NMGVAVQYLD PRGRLRSADS ENALTVQERN 350
VPTKSPSAPI NWRRGKLLGQ GAFGRVYLCY DVDTGRELAS KQVQFDPDSP 400
ETSKEVSALE CEIQLLKNLQ HERIVQYYGC LRDRAEKILT IFMEYMPGGS 450
VKDQLKAYGA LTESVTRKYT RQILEGMSYL HSNMIVHRDI KGANILRDSA 500
GNVKLGDFGA SKRLQTICMS GTGIRSVTGT PYWMSPEVIS GEGYGRKADV 550
WSLGCTVVEM LTEKPPWAEY EAMAAIFKIA TQPTNPQLPS HISEHGRDFL 600
RRIFVEARQR PSAEELLTHH FAQLVY 626
Length:626
Mass (Da):70,776
Last modified:November 1, 1996 - v1
Checksum:i00EF2442C9E56E0B
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U43187 mRNA. Translation: AAB03535.1.
BC023781 mRNA. Translation: AAH23781.1.
CCDSiCCDS25548.1.
RefSeqiNP_036077.1. NM_011947.3.
UniGeneiMm.27041.

Genome annotation databases

EnsembliENSMUST00000002044; ENSMUSP00000002044; ENSMUSG00000020700.
GeneIDi26406.
KEGGimmu:26406.
UCSCiuc007lyc.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U43187 mRNA. Translation: AAB03535.1 .
BC023781 mRNA. Translation: AAH23781.1 .
CCDSi CCDS25548.1.
RefSeqi NP_036077.1. NM_011947.3.
UniGenei Mm.27041.

3D structure databases

ProteinModelPortali Q61084.
SMRi Q61084. Positions 42-126, 336-624.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 204959. 1 interaction.
IntActi Q61084. 4 interactions.
MINTi MINT-1531397.

PTM databases

PhosphoSitei Q61084.

Proteomic databases

PaxDbi Q61084.
PRIDEi Q61084.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000002044 ; ENSMUSP00000002044 ; ENSMUSG00000020700 .
GeneIDi 26406.
KEGGi mmu:26406.
UCSCi uc007lyc.1. mouse.

Organism-specific databases

CTDi 4215.
MGIi MGI:1346874. Map3k3.

Phylogenomic databases

eggNOGi COG0515.
GeneTreei ENSGT00750000117243.
HOGENOMi HOG000049130.
HOVERGENi HBG006303.
InParanoidi Q61084.
KOi K04421.
OMAi QALHSIM.
OrthoDBi EOG747PHJ.
PhylomeDBi Q61084.
TreeFami TF105113.

Enzyme and pathway databases

BRENDAi 2.7.12.2. 3474.
Reactomei REACT_224208. Interleukin-1 signaling.

Miscellaneous databases

ChiTaRSi MAP3K3. mouse.
NextBioi 304381.
PROi Q61084.
SOURCEi Search...

Gene expression databases

Bgeei Q61084.
CleanExi MM_MAP3K3.
Genevestigatori Q61084.

Family and domain databases

InterProi IPR011009. Kinase-like_dom.
IPR000270. OPR_PB1.
IPR000719. Prot_kinase_dom.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
[Graphical view ]
Pfami PF00564. PB1. 1 hit.
PF00069. Pkinase. 1 hit.
[Graphical view ]
SMARTi SM00666. PB1. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view ]
SUPFAMi SSF56112. SSF56112. 1 hit.
PROSITEi PS50011. PROTEIN_KINASE_DOM. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning of mitogen-activated protein/ERK kinase kinases (MEKK) 2 and 3. Regulation of sequential phosphorylation pathways involving mitogen-activated protein kinase and c-Jun kinase."
    Blank J.L., Gerwins P., Elliott E.M., Sather S., Johnson G.L.
    J. Biol. Chem. 271:5361-5368(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION.
    Tissue: Brain.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Mammary gland.
  3. "JLP: a scaffolding protein that tethers JNK/p38MAPK signaling modules and transcription factors."
    Lee C.M., Onesime D., Reddy C.D., Dhanasekaran N., Reddy E.P.
    Proc. Natl. Acad. Sci. U.S.A. 99:14189-14194(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH SPAG9.
  4. Cited for: INTERACTION WITH MAP2K3; RAC1 AND CCM2.
  5. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-337 AND SER-340, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.

Entry informationi

Entry nameiM3K3_MOUSE
AccessioniPrimary (citable) accession number: Q61084
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: September 3, 2014
This is version 128 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi