Reviewed,
UniProtKB/Swiss-Prot Q61084 (M3K3_MOUSE)
Last modified
February 9, 2010.
Version 88.
History...
Clusters with 100%,
90%,
50% identity |
Documents (3) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Mitogen-activated protein kinase kinase kinase 3 EC=2.7.11.25 Alternative name(s): MAPK/ERK kinase kinase 3 Short name=MEK kinase 3 Short name=MEKK 3 | ||||
| Gene names |
| ||||
| Organism | Mus musculus (Mouse) | ||||
| Taxonomic identifier | 10090 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus |
Protein attributes
| Sequence length | 626 AA. |
| Sequence status | Complete. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Component of a protein kinase signal transduction cascade. Mediates activation of the NF-kappa-B, AP1 and DDIT3 transcriptional regulators. Ref.1 |
| Catalytic activity | ATP + a protein = ADP + a phosphoprotein. |
| Cofactor | Magnesium. |
| Enzyme regulation | Activated by phosphorylation on Thr-530 By similarity. |
| Subunit structure | Binds both upstream activators and downstream substrates in multimolecular complexes. Part of a complex with MAP2K3, RAC1 and CCM2. Interacts with MAP2K5 and SPAG9. Ref.3 Ref.4 |
| Sequence similarities | Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily. Contains 1 OPR domain. Contains 1 protein kinase domain. |
Ontologies
| Keywords | |
|---|---|
| Ligand | ATP-binding Magnesium Metal-binding Nucleotide-binding |
| Molecular function | Kinase Serine/threonine-protein kinase Transferase |
| PTM | Phosphoprotein |
| Gene Ontology (GO) | |
| Biological process | protein amino acid phosphorylation Inferred from electronic annotation. Source: InterPro |
| Molecular function | ATP binding Inferred from electronic annotation. Source: UniProtKB-KW MAP kinase kinase kinase activityInferred from electronic annotation. Source: EC magnesium ion bindingInferred from electronic annotation. Source: UniProtKB-KW protein bindingInferred from physical interaction. Source: IntAct |
| Complete GO annotation... | |
Binary interactions
With | Entry | #Exp. | IntAct | Notes |
|---|---|---|---|---|
| Map2k5 | Q9WVS7 | 4 | EBI-446250,EBI-446144 | |
| Traf6 | P70196 | 1 | EBI-446250,EBI-448028 |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 626 | 626 | Mitogen-activated protein kinase kinase kinase 3 | PRO_0000086246 | |||||
Regions | |||||||||
| Domain | 44 – 123 | 80 | OPR | ||||||
| Domain | 362 – 622 | 261 | Protein kinase | ||||||
| Nucleotide binding | 368 – 376 | 9 | ATP By similarity | ||||||
Sites | |||||||||
| Active site | 489 | 1 | Proton acceptor By similarity | ||||||
| Binding site | 391 | 1 | ATP By similarity | ||||||
Amino acid modifications | |||||||||
| Modified residue | 129 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 130 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 131 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 145 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 147 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 166 | 1 | Phosphoserine Ref.6 | ||||||
| Modified residue | 168 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 175 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 176 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 234 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 237 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 250 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 288 | 1 | Phosphotyrosine By similarity | ||||||
| Modified residue | 294 | 1 | Phosphothreonine By similarity | ||||||
| Modified residue | 311 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 314 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 316 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 317 | 1 | Phosphothreonine By similarity | ||||||
| Modified residue | 337 | 1 | Phosphoserine Ref.5 | ||||||
| Modified residue | 340 | 1 | Phosphoserine Ref.5 | ||||||
| Modified residue | 353 | 1 | Phosphothreonine By similarity | ||||||
| Modified residue | 355 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 464 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 526 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 530 | 1 | Phosphothreonine By similarity | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Molecular cloning of mitogen-activated protein/ERK kinase kinases (MEKK) 2 and 3. Regulation of sequential phosphorylation pathways involving mitogen-activated protein kinase and c-Jun kinase." Blank J.L., Gerwins P., Elliott E.M., Sather S., Johnson G.L. J. Biol. Chem. 271:5361-5368(1996) [PubMed: 8621389] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION. Tissue: Brain. |
| [2] | "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: FVB/N. Tissue: Mammary gland. |
| [3] | "JLP: a scaffolding protein that tethers JNK/p38MAPK signaling modules and transcription factors." Lee C.M., Onesime D., Reddy C.D., Dhanasekaran N., Reddy E.P. Proc. Natl. Acad. Sci. U.S.A. 99:14189-14194(2002) [PubMed: 12391307] [Abstract] Cited for: INTERACTION WITH SPAG9. |
| [4] | "Rac-MEKK3-MKK3 scaffolding for p38 MAPK activation during hyperosmotic shock." Uhlik M.T., Abell A.N., Johnson N.L., Sun W., Cuevas B.D., Lobel-Rice K.E., Horne E.A., Dell'Acqua M.L., Johnson G.L. Nat. Cell Biol. 5:1104-1110(2003) [PubMed: 14634666] [Abstract] Cited for: INTERACTION WITH MAP2K3; RAC1 AND CCM2. |
| [5] | "Large-scale phosphorylation analysis of mouse liver." Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed: 17242355] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-337 AND SER-340, MASS SPECTROMETRY. Tissue: Liver. |
| [6] | "Solid tumor proteome and phosphoproteome analysis by high resolution mass spectrometry." Zanivan S., Gnad F., Wickstroem S.A., Geiger T., Macek B., Cox J., Faessler R., Mann M. J. Proteome Res. 7:5314-5326(2008) [PubMed: 18973353] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-166, MASS SPECTROMETRY. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | U43187 mRNA. Translation: AAB03535.1. BC023781 mRNA. Translation: AAH23781.1. |
| IPI | IPI00117089. |
| RefSeq | NP_036077.1. |
| UniGene | Mm.27041 |
3D structure databases | |
| SMR | Q61084. Positions 42-126, 361-622. |
| ModBase | Search... |
Protein-protein interaction databases | |
| IntAct | Q61084. 3 interactions. |
| STRING | Q61084. |
PTM databases | |
| PhosphoSite | Q61084. |
Proteomic databases | |
| PRIDE | Q61084. |
Genome annotation databases | |
| Ensembl | ENSMUST00000002044; ENSMUSP00000002044; ENSMUSG00000020700; Mus musculus. [Genome view] |
| GeneID | 26406. |
| KEGG | mmu:26406. |
| UCSC | uc007lyc.1. mouse. |
Organism-specific databases | |
| CTD | 26406. |
| MGI | MGI:1346874. Map3k3. |
Phylogenomic databases | |
| HOGENOM | HBG446160. |
| HOVERGEN | Q61084. |
| InParanoid | Q61084. |
| OMA | SGYETMK. |
| OrthoDB | EOG9PRW95. |
Enzyme and pathway databases | |
| BRENDA | 2.7.11.25. 244. 2.7.12.2. 244. |
Gene expression databases | |
| ArrayExpress | Q61084. |
| Bgee | Q61084. |
| CleanEx | MM_MAP3K3. |
| Genevestigator | Q61084. |
| GermOnline | ENSMUSG00000020700. Mus musculus. |
Family and domain databases | |
| InterPro | IPR011009. Kinase-like_dom. IPR015748. MAPKKK3. IPR000270. OPR_PB1. IPR000719. Prot_kinase_cat_dom. IPR017442. Se/Thr_prot_kinase-like_dom. IPR002290. Ser/Thr_prot_kinase_dom. [Graphical view] |
| PANTHER | PTHR22986:SF64. MAPKKK3. 1 hit. |
| Pfam | PF00564. PB1. 1 hit. PF00069. Pkinase. 1 hit. [Graphical view] |
| SMART | SM00666. PB1. 1 hit. SM00220. S_TKc. 1 hit. [Graphical view] |
| PROSITE | PS00107. PROTEIN_KINASE_ATP. False negative. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. False negative. [Graphical view] |
| ProtoNet | Search... |
Other Resources | |
| NextBio | 304381. |
| SOURCE | Search... |
Entry information
| Entry name | M3K3_MOUSE | ||||||||
| Accession | Primary (citable) accession number: Q61084 | ||||||||
| Entry history |
| ||||||||
| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||
Relevant documents
| MGD cross-references Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot |
| Human and mouse protein kinases Human and mouse protein kinases: classification and index |
| SIMILARITY comments Index of protein domains and families |

Clusters with


