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Q61079

- SIM2_MOUSE

UniProt

Q61079 - SIM2_MOUSE

Protein

Single-minded homolog 2

Gene

Sim2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 134 (01 Oct 2014)
      Sequence version 1 (01 Nov 1997)
      Previous versions | rss
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    Functioni

    Transcription factor that may be a master gene of CNS development in cooperation with Arnt. It may have pleiotropic effects in the tissues expressed during development.

    GO - Molecular functioni

    1. DNA binding Source: MGI
    2. protein binding Source: MGI
    3. protein heterodimerization activity Source: UniProtKB
    4. sequence-specific DNA binding transcription factor activity Source: InterPro
    5. signal transducer activity Source: InterPro

    GO - Biological processi

    1. cell differentiation Source: UniProtKB-KW
    2. embryonic pattern specification Source: MGI
    3. lung development Source: MGI
    4. negative regulation of transcription, DNA-templated Source: MGI
    5. negative regulation of transcription from RNA polymerase II promoter Source: MGI
    6. nervous system development Source: UniProtKB-KW
    7. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Molecular functioni

    Developmental protein

    Keywords - Biological processi

    Differentiation, Neurogenesis, Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Single-minded homolog 2
    Alternative name(s):
    SIM transcription factor
    Short name:
    mSIM
    Gene namesi
    Name:Sim2
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 16

    Organism-specific databases

    MGIiMGI:98307. Sim2.

    Subcellular locationi

    Nucleus PROSITE-ProRule annotation

    GO - Cellular componenti

    1. nucleus Source: MGI

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 657657Single-minded homolog 2PRO_0000127442Add
    BLAST

    Proteomic databases

    PRIDEiQ61079.

    PTM databases

    PhosphoSiteiQ61079.

    Expressioni

    Tissue specificityi

    Transcripts were detected in high levels in kidney followed by skeletal muscle and lung. Low levels were found in testis, brain and heart. In early fetal development it is found in CNS, developing kidney, tongue epithelium and cartilage primordia.

    Gene expression databases

    BgeeiQ61079.
    CleanExiMM_SIM2.
    GenevestigatoriQ61079.

    Interactioni

    Subunit structurei

    Efficient DNA binding requires dimerization with another bHLH protein. Heterodimer of SIM2 and ARNT.1 Publication

    Protein-protein interaction databases

    IntActiQ61079. 2 interactions.

    Structurei

    3D structure databases

    ProteinModelPortaliQ61079.
    SMRiQ61079. Positions 2-333.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini1 – 5353bHLHPROSITE-ProRule annotationAdd
    BLAST
    Domaini77 – 14771PAS 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini218 – 28871PACAdd
    BLAST
    Domaini218 – 28871PAS 2PROSITE-ProRule annotationAdd
    BLAST
    Domaini336 – 657322Single-minded C-terminalPROSITE-ProRule annotationAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi367 – 38620Nuclear localization signalBy similarityAdd
    BLAST

    Sequence similaritiesi

    Contains 1 bHLH (basic helix-loop-helix) domain.PROSITE-ProRule annotation
    Contains 2 PAS (PER-ARNT-SIM) domains.PROSITE-ProRule annotation
    Contains 1 Single-minded C-terminal domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiNOG310284.
    GeneTreeiENSGT00730000110507.
    HOGENOMiHOG000236298.
    HOVERGENiHBG003936.
    InParanoidiQ61079.
    KOiK09100.
    OMAiSDLLYAP.
    OrthoDBiEOG790G0R.
    PhylomeDBiQ61079.
    TreeFamiTF317772.

    Family and domain databases

    InterProiIPR011598. bHLH_dom.
    IPR001610. PAC.
    IPR000014. PAS.
    IPR013767. PAS_fold.
    IPR013655. PAS_fold_3.
    IPR010578. SIM_C.
    [Graphical view]
    PfamiPF00989. PAS. 1 hit.
    PF08447. PAS_3. 1 hit.
    PF06621. SIM_C. 1 hit.
    [Graphical view]
    SMARTiSM00353. HLH. 1 hit.
    SM00086. PAC. 1 hit.
    SM00091. PAS. 2 hits.
    [Graphical view]
    SUPFAMiSSF47459. SSF47459. 1 hit.
    SSF55785. SSF55785. 2 hits.
    PROSITEiPS50888. BHLH. 1 hit.
    PS50112. PAS. 2 hits.
    PS51302. SIM_C. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q61079-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKEKSKNAAK TRREKENGEF YELAKLLPLP SAITSQLDKA SIIRLTTSYL    50
    KMRAVFPEGL GDAWGQPSRT GPLDSVAKEL GSHLLQTLDG FVFVVASDGK 100
    IMYISETASV HLGLSQVELT GNSIYEYIHP SDHDEMTAVL TAHPPLHHHL 150
    LQEYEIERSF FLRMKCVLAK RNAGLTCSGY KVIHCSGYLK IRQYMLDMSL 200
    YDSCYQIVGL VAVGQSLPPS AITEIKLHSN MFMFRASLDL KLIFLDSRVT 250
    ELTGYEPQDL IEKTLYHHVH GCDTFHLRYA HHLLLVKGQV TTKYYRLLSK 300
    LGGWVWVQSY ATVVHNSRSS RPHCIVSVNY VLTDVEYKEL QLSLDQVSTS 350
    KSQESWRTTL STSQETRKSA KPKNTKMKTK LRTNPYPPQQ YSSFQMDKLE 400
    CSQVGNWRTS PPTNAVAPPE QQLHSEASDL LYGPPYSLPF SYHYGHFPLD 450
    SHVFSSKKPG LPAKFGQPQG SPCEVARFFL STLPASSECQ WHCANSLVPS 500
    SSSPAKNLSE PSPVNAARHG LVPNYEAPSA AARRFCEDPA PPSFPSCGHY 550
    REEPALGPAK APRQASRDAA RLALARAPPE CCAPPAPEPQ APAQLPFVLL 600
    NYHRVLARRG PLGSAAPGAP EAAGSLRPRH PGPVAASAPG APRPHYLGAS 650
    VIITNGR 657
    Length:657
    Mass (Da):72,513
    Last modified:November 1, 1997 - v1
    Checksum:iC7904CD24C0ABBAF
    GO

    Sequence cautioni

    The sequence AAA91202.1 differs from that shown. Reason: Frameshift at positions 382, 390, 430 and 641.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti263 – 2631K → R in BAA09700. (PubMed:8561800)Curated
    Sequence conflicti263 – 2631K → R in AAB84099. (PubMed:8812055)Curated
    Sequence conflicti336 – 3361E → G in AAA91202. (PubMed:8812055)Curated
    Sequence conflicti501 – 5011S → T in AAA91202. (PubMed:8812055)Curated
    Sequence conflicti512 – 5121S → P in AAB84099. (PubMed:8812055)Curated
    Sequence conflicti541 – 5411P → R in AAA91202. (PubMed:8812055)Curated
    Sequence conflicti561 – 58525APRQA…CCAPP → VLARRPGRARCMWES in AAA91202. (PubMed:8812055)CuratedAdd
    BLAST
    Sequence conflicti590 – 5912QA → HG in AAA91202. (PubMed:8812055)Curated
    Sequence conflicti638 – 6381A → R in BAA09700. (PubMed:8561800)Curated
    Sequence conflicti638 – 6381A → R in AAB84099. (PubMed:8812055)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U42554 mRNA. Translation: AAB19098.1.
    D63383 mRNA. Translation: BAA09700.1.
    U40576 mRNA. Translation: AAA91202.1. Frameshift.
    AF023873
    , AF023864, AF023865, AF023869, AF023871, AF023870, AF023868, AF023867, AF023866, AF023872 Genomic DNA. Translation: AAB84099.1.
    D64135 mRNA. Translation: BAA11013.1.
    CCDSiCCDS37406.1.
    RefSeqiNP_035507.2. NM_011377.2.
    UniGeneiMm.4775.

    Genome annotation databases

    EnsembliENSMUST00000072182; ENSMUSP00000072043; ENSMUSG00000062713.
    GeneIDi20465.
    KEGGimmu:20465.
    UCSCiuc008aae.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U42554 mRNA. Translation: AAB19098.1 .
    D63383 mRNA. Translation: BAA09700.1 .
    U40576 mRNA. Translation: AAA91202.1 . Frameshift.
    AF023873
    , AF023864 , AF023865 , AF023869 , AF023871 , AF023870 , AF023868 , AF023867 , AF023866 , AF023872 Genomic DNA. Translation: AAB84099.1 .
    D64135 mRNA. Translation: BAA11013.1 .
    CCDSi CCDS37406.1.
    RefSeqi NP_035507.2. NM_011377.2.
    UniGenei Mm.4775.

    3D structure databases

    ProteinModelPortali Q61079.
    SMRi Q61079. Positions 2-333.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q61079. 2 interactions.

    PTM databases

    PhosphoSitei Q61079.

    Proteomic databases

    PRIDEi Q61079.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000072182 ; ENSMUSP00000072043 ; ENSMUSG00000062713 .
    GeneIDi 20465.
    KEGGi mmu:20465.
    UCSCi uc008aae.1. mouse.

    Organism-specific databases

    CTDi 6493.
    MGIi MGI:98307. Sim2.

    Phylogenomic databases

    eggNOGi NOG310284.
    GeneTreei ENSGT00730000110507.
    HOGENOMi HOG000236298.
    HOVERGENi HBG003936.
    InParanoidi Q61079.
    KOi K09100.
    OMAi SDLLYAP.
    OrthoDBi EOG790G0R.
    PhylomeDBi Q61079.
    TreeFami TF317772.

    Miscellaneous databases

    NextBioi 298563.
    PROi Q61079.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q61079.
    CleanExi MM_SIM2.
    Genevestigatori Q61079.

    Family and domain databases

    InterProi IPR011598. bHLH_dom.
    IPR001610. PAC.
    IPR000014. PAS.
    IPR013767. PAS_fold.
    IPR013655. PAS_fold_3.
    IPR010578. SIM_C.
    [Graphical view ]
    Pfami PF00989. PAS. 1 hit.
    PF08447. PAS_3. 1 hit.
    PF06621. SIM_C. 1 hit.
    [Graphical view ]
    SMARTi SM00353. HLH. 1 hit.
    SM00086. PAC. 1 hit.
    SM00091. PAS. 2 hits.
    [Graphical view ]
    SUPFAMi SSF47459. SSF47459. 1 hit.
    SSF55785. SSF55785. 2 hits.
    PROSITEi PS50888. BHLH. 1 hit.
    PS50112. PAS. 2 hits.
    PS51302. SIM_C. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Characterization of msim, a murine homologue of the Drosophila sim transcription factor."
      Moffett P., Dayo M., Reece M., McCormack M.K., Pelletier J.
      Genomics 35:144-155(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Fetal kidney.
    2. "cDNA cloning of a murine homologue of Drosophila single-minded, its mRNA expression in mouse development, and chromosome localization."
      Ema M., Suzuki M., Morita M., Hirose K., Sogawa K., Matsuda Y., Gotoh O., Saijoh Y., Fujii H., Hamada H., Fujii-Kuriyama Y.
      Biochem. Biophys. Res. Commun. 218:588-594(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: C57BL/6J.
    3. "Expression patterns of two murine homologs of Drosophila single-minded suggest possible roles in embryonic patterning and in the pathogenesis of Down syndrome."
      Fan C.-M., Kuwana E., Bulfone A., Fletcher C.F., Copeland N.G., Jenkins N.A., Crews S., Martinez S., Puelles L., Rubenstein J.L., Tessier-Lavigne M.
      Mol. Cell. Neurosci. 7:1-16(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
      Strain: 129/Sv and Swiss Webster.
      Tissue: Embryonic brain.
    4. "The mammalian single-minded (SIM) gene: mouse cDNA structure and diencephalic expression indicate a candidate gene for Down syndrome."
      Yamaki A., Noda S., Kudoh J., Shindoh N., Maeda H., Minoshima S., Kawasaki K., Shimizu Y., Shimizu N.
      Genomics 35:136-143(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: ICR X Swiss Webster.
      Tissue: Embryo.
    5. "Two murine homologs of the Drosophila single-minded protein that interact with the mouse aryl hydrocarbon receptor nuclear translocator protein."
      Probst M.R., Fan C.-M., Tessier-Lavigne M., Hankinson O.
      J. Biol. Chem. 272:4451-4457(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBUNIT.

    Entry informationi

    Entry nameiSIM2_MOUSE
    AccessioniPrimary (citable) accession number: Q61079
    Secondary accession number(s): O35391, Q61046, Q61904
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: November 1, 1997
    Last modified: October 1, 2014
    This is version 134 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3