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Q61079 (SIM2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 133. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Single-minded homolog 2
Alternative name(s):
SIM transcription factor
Short name=mSIM
Gene names
Name:Sim2
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length657 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Transcription factor that may be a master gene of CNS development in cooperation with Arnt. It may have pleiotropic effects in the tissues expressed during development.

Subunit structure

Efficient DNA binding requires dimerization with another bHLH protein. Heterodimer of SIM2 and ARNT. Ref.5

Subcellular location

Nucleus By similarity.

Tissue specificity

Transcripts were detected in high levels in kidney followed by skeletal muscle and lung. Low levels were found in testis, brain and heart. In early fetal development it is found in CNS, developing kidney, tongue epithelium and cartilage primordia.

Sequence similarities

Contains 1 bHLH (basic helix-loop-helix) domain.

Contains 1 PAC (PAS-associated C-terminal) domain.

Contains 2 PAS (PER-ARNT-SIM) domains.

Contains 1 Single-minded C-terminal domain.

Sequence caution

The sequence AAA91202.1 differs from that shown. Reason: Frameshift at positions 382, 390, 430 and 641.

Ontologies

Keywords
   Biological processDifferentiation
Neurogenesis
Transcription
Transcription regulation
   Cellular componentNucleus
   DomainRepeat
   LigandDNA-binding
   Molecular functionDevelopmental protein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcell differentiation

Inferred from electronic annotation. Source: UniProtKB-KW

embryonic pattern specification

Inferred from mutant phenotype PubMed 12024028. Source: MGI

lung development

Inferred from mutant phenotype PubMed 12024028. Source: MGI

negative regulation of transcription from RNA polymerase II promoter

Inferred from genetic interaction PubMed 14701734. Source: MGI

negative regulation of transcription, DNA-templated

Inferred from direct assay PubMed 11782478. Source: MGI

nervous system development

Inferred from electronic annotation. Source: UniProtKB-KW

transcription, DNA-templated

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentnucleus

Inferred from direct assay PubMed 11782478. Source: MGI

   Molecular_functionDNA binding

Inferred from direct assay PubMed 11782478. Source: MGI

protein binding

Inferred from physical interaction PubMed 11782478. Source: MGI

protein heterodimerization activity

Inferred from physical interaction PubMed 8927054. Source: UniProtKB

sequence-specific DNA binding transcription factor activity

Inferred from electronic annotation. Source: InterPro

signal transducer activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 657657Single-minded homolog 2
PRO_0000127442

Regions

Domain1 – 5353bHLH
Domain77 – 14771PAS 1
Domain218 – 28871PAC
Domain218 – 28871PAS 2
Domain336 – 657322Single-minded C-terminal
Motif367 – 38620Nuclear localization signal By similarity

Experimental info

Sequence conflict2631K → R in BAA09700. Ref.2
Sequence conflict2631K → R in AAB84099. Ref.3
Sequence conflict3361E → G in AAA91202. Ref.3
Sequence conflict5011S → T in AAA91202. Ref.3
Sequence conflict5121S → P in AAB84099. Ref.3
Sequence conflict5411P → R in AAA91202. Ref.3
Sequence conflict561 – 58525APRQA…CCAPP → VLARRPGRARCMWES in AAA91202. Ref.3
Sequence conflict590 – 5912QA → HG in AAA91202. Ref.3
Sequence conflict6381A → R in BAA09700. Ref.2
Sequence conflict6381A → R in AAB84099. Ref.3

Sequences

Sequence LengthMass (Da)Tools
Q61079 [UniParc].

Last modified November 1, 1997. Version 1.
Checksum: C7904CD24C0ABBAF

FASTA65772,513
        10         20         30         40         50         60 
MKEKSKNAAK TRREKENGEF YELAKLLPLP SAITSQLDKA SIIRLTTSYL KMRAVFPEGL 

        70         80         90        100        110        120 
GDAWGQPSRT GPLDSVAKEL GSHLLQTLDG FVFVVASDGK IMYISETASV HLGLSQVELT 

       130        140        150        160        170        180 
GNSIYEYIHP SDHDEMTAVL TAHPPLHHHL LQEYEIERSF FLRMKCVLAK RNAGLTCSGY 

       190        200        210        220        230        240 
KVIHCSGYLK IRQYMLDMSL YDSCYQIVGL VAVGQSLPPS AITEIKLHSN MFMFRASLDL 

       250        260        270        280        290        300 
KLIFLDSRVT ELTGYEPQDL IEKTLYHHVH GCDTFHLRYA HHLLLVKGQV TTKYYRLLSK 

       310        320        330        340        350        360 
LGGWVWVQSY ATVVHNSRSS RPHCIVSVNY VLTDVEYKEL QLSLDQVSTS KSQESWRTTL 

       370        380        390        400        410        420 
STSQETRKSA KPKNTKMKTK LRTNPYPPQQ YSSFQMDKLE CSQVGNWRTS PPTNAVAPPE 

       430        440        450        460        470        480 
QQLHSEASDL LYGPPYSLPF SYHYGHFPLD SHVFSSKKPG LPAKFGQPQG SPCEVARFFL 

       490        500        510        520        530        540 
STLPASSECQ WHCANSLVPS SSSPAKNLSE PSPVNAARHG LVPNYEAPSA AARRFCEDPA 

       550        560        570        580        590        600 
PPSFPSCGHY REEPALGPAK APRQASRDAA RLALARAPPE CCAPPAPEPQ APAQLPFVLL 

       610        620        630        640        650 
NYHRVLARRG PLGSAAPGAP EAAGSLRPRH PGPVAASAPG APRPHYLGAS VIITNGR 

« Hide

References

[1]"Characterization of msim, a murine homologue of the Drosophila sim transcription factor."
Moffett P., Dayo M., Reece M., McCormack M.K., Pelletier J.
Genomics 35:144-155(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Fetal kidney.
[2]"cDNA cloning of a murine homologue of Drosophila single-minded, its mRNA expression in mouse development, and chromosome localization."
Ema M., Suzuki M., Morita M., Hirose K., Sogawa K., Matsuda Y., Gotoh O., Saijoh Y., Fujii H., Hamada H., Fujii-Kuriyama Y.
Biochem. Biophys. Res. Commun. 218:588-594(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: C57BL/6J.
[3]"Expression patterns of two murine homologs of Drosophila single-minded suggest possible roles in embryonic patterning and in the pathogenesis of Down syndrome."
Fan C.-M., Kuwana E., Bulfone A., Fletcher C.F., Copeland N.G., Jenkins N.A., Crews S., Martinez S., Puelles L., Rubenstein J.L., Tessier-Lavigne M.
Mol. Cell. Neurosci. 7:1-16(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
Strain: 129/Sv and Swiss Webster.
Tissue: Embryonic brain.
[4]"The mammalian single-minded (SIM) gene: mouse cDNA structure and diencephalic expression indicate a candidate gene for Down syndrome."
Yamaki A., Noda S., Kudoh J., Shindoh N., Maeda H., Minoshima S., Kawasaki K., Shimizu Y., Shimizu N.
Genomics 35:136-143(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: ICR X Swiss Webster.
Tissue: Embryo.
[5]"Two murine homologs of the Drosophila single-minded protein that interact with the mouse aryl hydrocarbon receptor nuclear translocator protein."
Probst M.R., Fan C.-M., Tessier-Lavigne M., Hankinson O.
J. Biol. Chem. 272:4451-4457(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBUNIT.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U42554 mRNA. Translation: AAB19098.1.
D63383 mRNA. Translation: BAA09700.1.
U40576 mRNA. Translation: AAA91202.1. Frameshift.
AF023873 expand/collapse EMBL AC list , AF023864, AF023865, AF023869, AF023871, AF023870, AF023868, AF023867, AF023866, AF023872 Genomic DNA. Translation: AAB84099.1.
D64135 mRNA. Translation: BAA11013.1.
CCDSCCDS37406.1.
RefSeqNP_035507.2. NM_011377.2.
UniGeneMm.4775.

3D structure databases

ProteinModelPortalQ61079.
SMRQ61079. Positions 2-333.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActQ61079. 2 interactions.

PTM databases

PhosphoSiteQ61079.

Proteomic databases

PRIDEQ61079.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000072182; ENSMUSP00000072043; ENSMUSG00000062713.
GeneID20465.
KEGGmmu:20465.
UCSCuc008aae.1. mouse.

Organism-specific databases

CTD6493.
MGIMGI:98307. Sim2.

Phylogenomic databases

eggNOGNOG310284.
GeneTreeENSGT00730000110507.
HOGENOMHOG000236298.
HOVERGENHBG003936.
InParanoidQ61079.
KOK09100.
OMASDLLYAP.
OrthoDBEOG790G0R.
PhylomeDBQ61079.
TreeFamTF317772.

Gene expression databases

BgeeQ61079.
CleanExMM_SIM2.
GenevestigatorQ61079.

Family and domain databases

InterProIPR011598. bHLH_dom.
IPR001610. PAC.
IPR000014. PAS.
IPR013767. PAS_fold.
IPR013655. PAS_fold_3.
IPR010578. SIM_C.
[Graphical view]
PfamPF00989. PAS. 1 hit.
PF08447. PAS_3. 1 hit.
PF06621. SIM_C. 1 hit.
[Graphical view]
SMARTSM00353. HLH. 1 hit.
SM00086. PAC. 1 hit.
SM00091. PAS. 2 hits.
[Graphical view]
SUPFAMSSF47459. SSF47459. 1 hit.
SSF55785. SSF55785. 2 hits.
PROSITEPS50888. BHLH. 1 hit.
PS50112. PAS. 2 hits.
PS51302. SIM_C. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio298563.
PROQ61079.
SOURCESearch...

Entry information

Entry nameSIM2_MOUSE
AccessionPrimary (citable) accession number: Q61079
Secondary accession number(s): O35391, Q61046, Q61904
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: July 9, 2014
This is version 133 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot