Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Disintegrin and metalloproteinase domain-containing protein 9

Gene

Adam9

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Probable zinc protease. May mediate cell-cell or cell-matrix interactions.

Cofactori

Zn2+CuratedNote: Binds 1 zinc ion per subunit.Curated

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi347 – 3471Zinc; catalyticBy similarity
Active sitei348 – 3481PROSITE-ProRule annotationBy similarity
Metal bindingi351 – 3511Zinc; catalyticBy similarity
Metal bindingi357 – 3571Zinc; catalyticBy similarity

GO - Molecular functioni

  • collagen binding Source: MGI
  • integrin binding Source: MGI
  • laminin binding Source: MGI
  • metalloendopeptidase activity Source: BHF-UCL
  • metallopeptidase activity Source: MGI
  • protein kinase C binding Source: BHF-UCL
  • SH3 domain binding Source: BHF-UCL
  • zinc ion binding Source: InterPro

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Metalloprotease, Protease

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BRENDAi3.4.24.B9. 3474.
ReactomeiREACT_313067. Collagen degradation.

Protein family/group databases

MEROPSiM12.209.

Names & Taxonomyi

Protein namesi
Recommended name:
Disintegrin and metalloproteinase domain-containing protein 9 (EC:3.4.24.-)
Short name:
ADAM 9
Alternative name(s):
Meltrin-gamma
Metalloprotease/disintegrin/cysteine-rich protein 9
Myeloma cell metalloproteinase
Gene namesi
Name:Adam9Imported
Synonyms:Kiaa0021Imported, Mdc9, Mltng
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 8

Organism-specific databases

MGIiMGI:105376. Adam9.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini30 – 697668ExtracellularSequence AnalysisAdd
BLAST
Transmembranei698 – 71821HelicalSequence AnalysisAdd
BLAST
Topological domaini719 – 845127CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  • cell surface Source: BHF-UCL
  • cytoplasm Source: MGI
  • extracellular exosome Source: MGI
  • extracellular space Source: BHF-UCL
  • focal adhesion Source: MGI
  • integral component of membrane Source: UniProtKB-KW
  • intrinsic component of external side of plasma membrane Source: BHF-UCL
  • plasma membrane Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2929Sequence AnalysisAdd
BLAST
Chaini30 – 845816Disintegrin and metalloproteinase domain-containing protein 9PRO_0000029063Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi144 – 1441N-linked (GlcNAc...)Sequence Analysis
Glycosylationi154 – 1541N-linked (GlcNAc...)Sequence Analysis
Glycosylationi231 – 2311N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi322 ↔ 401By similarity
Disulfide bondi363 ↔ 385By similarity
Disulfide bondi365 ↔ 370By similarity
Glycosylationi381 – 3811N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi473 ↔ 493By similarity
Glycosylationi487 – 4871N-linked (GlcNAc...)Sequence Analysis
Glycosylationi636 – 6361N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi644 ↔ 656By similarity
Disulfide bondi650 ↔ 662By similarity
Disulfide bondi664 ↔ 673By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiQ61072.
PaxDbiQ61072.
PRIDEiQ61072.

PTM databases

PhosphoSiteiQ61072.

Expressioni

Gene expression databases

BgeeiQ61072.
ExpressionAtlasiQ61072. baseline and differential.
GenevisibleiQ61072. MM.

Interactioni

Subunit structurei

Interacts with SH3GL2 and SNX9 through its cytoplasmic tail.1 Publication

Protein-protein interaction databases

IntActiQ61072. 2 interactions.
MINTiMINT-252904.
STRINGi10090.ENSMUSP00000081048.

Structurei

3D structure databases

ProteinModelPortaliQ61072.
SMRiQ61072. Positions 212-638.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini212 – 406195Peptidase M12BPROSITE-ProRule annotationAdd
BLAST
Domaini414 – 50188DisintegrinPROSITE-ProRule annotationAdd
BLAST
Domaini644 – 69855EGF-likePROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi505 – 634130Cys-richAdd
BLAST

Sequence similaritiesi

Contains 1 disintegrin domain.PROSITE-ProRule annotation
Contains 1 EGF-like domain.PROSITE-ProRule annotation
Contains 1 peptidase M12B domain.PROSITE-ProRule annotation

Keywords - Domaini

EGF-like domain, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG294463.
GeneTreeiENSGT00760000118888.
HOGENOMiHOG000230883.
HOVERGENiHBG006978.
InParanoidiQ61072.
OMAiSSQFCQP.
OrthoDBiEOG7F7W89.
TreeFamiTF314733.

Family and domain databases

Gene3Di3.40.390.10. 1 hit.
4.10.70.10. 1 hit.
InterProiIPR006586. ADAM_Cys-rich.
IPR001762. Blood-coag_inhib_Disintegrin.
IPR018358. Disintegrin_CS.
IPR000742. EG-like_dom.
IPR013032. EGF-like_CS.
IPR024079. MetalloPept_cat_dom.
IPR001590. Peptidase_M12B.
IPR002870. Peptidase_M12B_N.
[Graphical view]
PfamiPF08516. ADAM_CR. 1 hit.
PF00200. Disintegrin. 1 hit.
PF01562. Pep_M12B_propep. 1 hit.
PF01421. Reprolysin. 1 hit.
[Graphical view]
PRINTSiPR00289. DISINTEGRIN.
SMARTiSM00608. ACR. 1 hit.
SM00050. DISIN. 1 hit.
SM00181. EGF. 1 hit.
[Graphical view]
SUPFAMiSSF57552. SSF57552. 1 hit.
PROSITEiPS50215. ADAM_MEPRO. 1 hit.
PS00427. DISINTEGRIN_1. 1 hit.
PS50214. DISINTEGRIN_2. 1 hit.
PS01186. EGF_2. 1 hit.
PS50026. EGF_3. 1 hit.
PS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q61072-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGPRALSPLA SLRLRWLLAC GLLGPVLEAG RPDLEQTVHL SSYEIITPWR
60 70 80 90 100
LTRERREALG PSSQQISYVI QAQGKQHIIH LERNTDLLPN DFVVYTYDKE
110 120 130 140 150
GSLLSDHPNV QSHCHYRGYV EGVQNSAVAV SACFGLRGLL HLENASFGIE
160 170 180 190 200
PLHNSSHFEH IFYPMDGIHQ EPLRCGVSNR DTEKEGTQGD EEEHPSVTQL
210 220 230 240 250
LRRRRAVLPQ TRYVELFIVV DKERYDMMGR NQTAVREEMI RLANYLDSMY
260 270 280 290 300
IMLNIRIVLV GLEIWTDRNP INIIGGAGDV LGNFVQWREK FLITRWRHDS
310 320 330 340 350
AQLVLKKGFG GTAGMAFVGT VCSRSHAGGI NVFGQITVET FASIVAHELG
360 370 380 390 400
HNLGMNHDDG RECFCGAKSC IMNSGASGSR NFSSCSAEDF EKLTLNKGGS
410 420 430 440 450
CLLNIPKPDE AYSAPSCGNK LVDPGEECDC GTAKECEVDP CCEGSTCKLK
460 470 480 490 500
SFAECAYGDC CKDCQFLPGG SMCRGKTSEC DVPEYCNGSS QFCPPDVFIQ
510 520 530 540 550
NGYPCQNSKA YCYNGMCQYY DAQCQVIFGS KAKAAPRDCF IEVNSKGDRF
560 570 580 590 600
GNCGFSGSEY KKCATGNALC GKLQCENVQD MPVFGIVPAI IQTPSRGTKC
610 620 630 640 650
WGVDFQLGSD VPDPGMVNEG TKCDAGKICR NFQCVNASVL NYDCDIQGKC
660 670 680 690 700
HGHGVCNSNK NCHCEDGWAP PHCDTKGYGG SVDSGPTYNA KSTALRDGLL
710 720 730 740 750
VFFFLIVPLV AAAIFLFIKR DELRKTFRKK RSQMSDGRNQ ANVSRQPGDP
760 770 780 790 800
SISRPPGGPN VSRPPGGPGV SRPPGGPGVS RPPGGPGVSR PPPGHGNRFP
810 820 830 840
VPTYAAKQPA QFPSRPPPPQ PKISSQGNLI PARPAPAPPL YSSLT
Length:845
Mass (Da):92,079
Last modified:July 27, 2011 - v2
Checksum:i38C40F89D4A77725
GO

Sequence cautioni

The sequence BAC65470.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti296 – 2961W → R in AAC52446 (PubMed:8647900).Curated
Sequence conflicti296 – 2961W → R in BAC65470 (PubMed:12693553).Curated
Sequence conflicti296 – 2961W → R in AAH47156 (PubMed:15489334).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U41765 mRNA. Translation: AAC52446.1.
AK122188 mRNA. Translation: BAC65470.1. Different initiation.
AC156553 Genomic DNA. No translation available.
BC047156 mRNA. Translation: AAH47156.1.
D50412 mRNA. Translation: BAA08913.1.
U06145 mRNA. Translation: AAA18424.1.
PIRiI48943.
S60259.
RefSeqiNP_031430.2. NM_007404.2.
UniGeneiMm.28908.

Genome annotation databases

EnsembliENSMUST00000084035; ENSMUSP00000081048; ENSMUSG00000031555.
GeneIDi11502.
UCSCiuc009lfk.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U41765 mRNA. Translation: AAC52446.1.
AK122188 mRNA. Translation: BAC65470.1. Different initiation.
AC156553 Genomic DNA. No translation available.
BC047156 mRNA. Translation: AAH47156.1.
D50412 mRNA. Translation: BAA08913.1.
U06145 mRNA. Translation: AAA18424.1.
PIRiI48943.
S60259.
RefSeqiNP_031430.2. NM_007404.2.
UniGeneiMm.28908.

3D structure databases

ProteinModelPortaliQ61072.
SMRiQ61072. Positions 212-638.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ61072. 2 interactions.
MINTiMINT-252904.
STRINGi10090.ENSMUSP00000081048.

Protein family/group databases

MEROPSiM12.209.

PTM databases

PhosphoSiteiQ61072.

Proteomic databases

MaxQBiQ61072.
PaxDbiQ61072.
PRIDEiQ61072.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000084035; ENSMUSP00000081048; ENSMUSG00000031555.
GeneIDi11502.
UCSCiuc009lfk.2. mouse.

Organism-specific databases

CTDi8754.
MGIiMGI:105376. Adam9.
RougeiSearch...

Phylogenomic databases

eggNOGiNOG294463.
GeneTreeiENSGT00760000118888.
HOGENOMiHOG000230883.
HOVERGENiHBG006978.
InParanoidiQ61072.
OMAiSSQFCQP.
OrthoDBiEOG7F7W89.
TreeFamiTF314733.

Enzyme and pathway databases

BRENDAi3.4.24.B9. 3474.
ReactomeiREACT_313067. Collagen degradation.

Miscellaneous databases

NextBioi278906.
PROiQ61072.
SOURCEiSearch...

Gene expression databases

BgeeiQ61072.
ExpressionAtlasiQ61072. baseline and differential.
GenevisibleiQ61072. MM.

Family and domain databases

Gene3Di3.40.390.10. 1 hit.
4.10.70.10. 1 hit.
InterProiIPR006586. ADAM_Cys-rich.
IPR001762. Blood-coag_inhib_Disintegrin.
IPR018358. Disintegrin_CS.
IPR000742. EG-like_dom.
IPR013032. EGF-like_CS.
IPR024079. MetalloPept_cat_dom.
IPR001590. Peptidase_M12B.
IPR002870. Peptidase_M12B_N.
[Graphical view]
PfamiPF08516. ADAM_CR. 1 hit.
PF00200. Disintegrin. 1 hit.
PF01562. Pep_M12B_propep. 1 hit.
PF01421. Reprolysin. 1 hit.
[Graphical view]
PRINTSiPR00289. DISINTEGRIN.
SMARTiSM00608. ACR. 1 hit.
SM00050. DISIN. 1 hit.
SM00181. EGF. 1 hit.
[Graphical view]
SUPFAMiSSF57552. SSF57552. 1 hit.
PROSITEiPS50215. ADAM_MEPRO. 1 hit.
PS00427. DISINTEGRIN_1. 1 hit.
PS50214. DISINTEGRIN_2. 1 hit.
PS01186. EGF_2. 1 hit.
PS50026. EGF_3. 1 hit.
PS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "MDC9, a widely expressed cellular disintegrin containing cytoplasmic SH3 ligand domains."
    Weskamp G., Kraetzschmar J., Reid M.S., Blobel C.P.
    J. Cell Biol. 132:717-726(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Lung.
  2. "Prediction of the coding sequences of mouse homologues of KIAA gene: II. The complete nucleotide sequences of 400 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
    Okazaki N., Kikuno R., Ohara R., Inamoto S., Aizawa H., Yuasa S., Nakajima D., Nagase T., Ohara O., Koga H.
    DNA Res. 10:35-48(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: BrainImported.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N-3.
    Tissue: Mammary gland.
  5. "A metalloprotease-disintegrin participating in myoblast fusion."
    Yagami-Hiromasa T., Sato T., Kurisaki T., Kamijo K., Nabeshima Y., Fujisawa-Sehara A.
    Nature 377:652-656(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 426-575.
  6. "A family of cellular proteins related to snake venom disintegrins."
    Weskamp G., Blobel C.P.
    Proc. Natl. Acad. Sci. U.S.A. 91:2748-2751(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 432-478.
    Strain: BALB/c.
  7. "Interaction of the metalloprotease disintegrins MDC9 and MDC15 with two SH3 domain-containing proteins, endophilin I and SH3PX1."
    Howard L., Nelson K.K., Maciewicz R.A., Blobel C.P.
    J. Biol. Chem. 274:31693-31699(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH SH3GL2 AND SNX9.

Entry informationi

Entry nameiADAM9_MOUSE
AccessioniPrimary (citable) accession number: Q61072
Secondary accession number(s): E9QPP2
, Q60618, Q61853, Q80U94
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2004
Last sequence update: July 27, 2011
Last modified: July 22, 2015
This is version 130 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.