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Q61072

- ADAM9_MOUSE

UniProt

Q61072 - ADAM9_MOUSE

Protein

Disintegrin and metalloproteinase domain-containing protein 9

Gene

Adam9

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 122 (01 Oct 2014)
      Sequence version 2 (27 Jul 2011)
      Previous versions | rss
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    Functioni

    Probable zinc protease. May mediate cell-cell or cell-matrix interactions.

    Cofactori

    Binds 1 zinc ion per subunit.Curated

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi347 – 3471Zinc; catalyticBy similarity
    Active sitei348 – 3481By similarityPROSITE-ProRule annotation
    Metal bindingi351 – 3511Zinc; catalyticBy similarity
    Metal bindingi357 – 3571Zinc; catalyticBy similarity

    GO - Molecular functioni

    1. metalloendopeptidase activity Source: BHF-UCL
    2. protein binding Source: BHF-UCL
    3. protein kinase C binding Source: BHF-UCL
    4. SH3 domain binding Source: BHF-UCL
    5. zinc ion binding Source: InterPro

    GO - Biological processi

    1. activation of MAPKK activity Source: Ensembl
    2. cell-cell adhesion mediated by integrin Source: Ensembl
    3. cell-matrix adhesion Source: Ensembl
    4. cellular response to lipopolysaccharide Source: Ensembl
    5. keratinocyte differentiation Source: Ensembl
    6. membrane protein ectodomain proteolysis Source: BHF-UCL
    7. monocyte activation Source: Ensembl
    8. PMA-inducible membrane protein ectodomain proteolysis Source: Ensembl
    9. positive regulation of cell adhesion mediated by integrin Source: Ensembl
    10. positive regulation of keratinocyte migration Source: Ensembl
    11. positive regulation of macrophage fusion Source: Ensembl
    12. positive regulation of membrane protein ectodomain proteolysis Source: BHF-UCL
    13. positive regulation of protein secretion Source: Ensembl
    14. response to calcium ion Source: Ensembl
    15. response to glucocorticoid Source: BHF-UCL
    16. response to hydrogen peroxide Source: Ensembl
    17. response to laminar fluid shear stress Source: Ensembl
    18. response to manganese ion Source: Ensembl
    19. response to tumor necrosis factor Source: Ensembl
    20. transforming growth factor beta receptor signaling pathway Source: BHF-UCL

    Keywords - Molecular functioni

    Hydrolase, Metalloprotease, Protease

    Keywords - Ligandi

    Metal-binding, Zinc

    Enzyme and pathway databases

    ReactomeiREACT_199055. Collagen degradation.

    Protein family/group databases

    MEROPSiM12.209.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Disintegrin and metalloproteinase domain-containing protein 9 (EC:3.4.24.-)
    Short name:
    ADAM 9
    Alternative name(s):
    Meltrin-gamma
    Metalloprotease/disintegrin/cysteine-rich protein 9
    Myeloma cell metalloproteinase
    Gene namesi
    Name:Adam9Imported
    Synonyms:Kiaa0021Imported, Mdc9, Mltng
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 8

    Organism-specific databases

    MGIiMGI:105376. Adam9.

    Subcellular locationi

    GO - Cellular componenti

    1. basolateral plasma membrane Source: Ensembl
    2. cell surface Source: BHF-UCL
    3. cytoplasm Source: MGI
    4. extracellular space Source: BHF-UCL
    5. integral component of membrane Source: UniProtKB-KW
    6. intrinsic component of external side of plasma membrane Source: BHF-UCL
    7. plasma membrane Source: MGI

    Keywords - Cellular componenti

    Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2929Sequence AnalysisAdd
    BLAST
    Chaini30 – 845816Disintegrin and metalloproteinase domain-containing protein 9PRO_0000029063Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi144 – 1441N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi154 – 1541N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi231 – 2311N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi322 ↔ 401By similarity
    Disulfide bondi363 ↔ 385By similarity
    Disulfide bondi365 ↔ 370By similarity
    Glycosylationi381 – 3811N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi473 ↔ 493By similarity
    Glycosylationi487 – 4871N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi636 – 6361N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi644 ↔ 656By similarity
    Disulfide bondi650 ↔ 662By similarity
    Disulfide bondi664 ↔ 673By similarity

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    PaxDbiQ61072.
    PRIDEiQ61072.

    PTM databases

    PhosphoSiteiQ61072.

    Expressioni

    Gene expression databases

    ArrayExpressiQ61072.
    BgeeiQ61072.
    GenevestigatoriQ61072.

    Interactioni

    Subunit structurei

    Interacts with SH3GL2 and SNX9 through its cytoplasmic tail.1 Publication

    Protein-protein interaction databases

    IntActiQ61072. 2 interactions.
    MINTiMINT-252904.

    Structurei

    3D structure databases

    ProteinModelPortaliQ61072.
    SMRiQ61072. Positions 212-638, 647-675.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini30 – 697668ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini719 – 845127CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei698 – 71821HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini212 – 406195Peptidase M12BPROSITE-ProRule annotationAdd
    BLAST
    Domaini414 – 50188DisintegrinPROSITE-ProRule annotationAdd
    BLAST
    Domaini644 – 69855EGF-likePROSITE-ProRule annotationAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi505 – 634130Cys-richAdd
    BLAST

    Sequence similaritiesi

    Contains 1 disintegrin domain.PROSITE-ProRule annotation
    Contains 1 EGF-like domain.PROSITE-ProRule annotation
    Contains 1 peptidase M12B domain.PROSITE-ProRule annotation

    Keywords - Domaini

    EGF-like domain, Signal, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG294463.
    GeneTreeiENSGT00750000117231.
    HOGENOMiHOG000230883.
    HOVERGENiHBG006978.
    InParanoidiQ61072.
    KOiK06834.
    OMAiFCQPDVF.
    OrthoDBiEOG7F7W89.
    TreeFamiTF314733.

    Family and domain databases

    Gene3Di3.40.390.10. 1 hit.
    4.10.70.10. 1 hit.
    InterProiIPR006586. ADAM_Cys-rich.
    IPR001762. Blood-coag_inhib_Disintegrin.
    IPR018358. Disintegrin_CS.
    IPR000742. EG-like_dom.
    IPR013032. EGF-like_CS.
    IPR024079. MetalloPept_cat_dom.
    IPR001590. Peptidase_M12B.
    IPR002870. Peptidase_M12B_N.
    [Graphical view]
    PfamiPF08516. ADAM_CR. 1 hit.
    PF00200. Disintegrin. 1 hit.
    PF01562. Pep_M12B_propep. 1 hit.
    PF01421. Reprolysin. 1 hit.
    [Graphical view]
    PRINTSiPR00289. DISINTEGRIN.
    SMARTiSM00608. ACR. 1 hit.
    SM00050. DISIN. 1 hit.
    SM00181. EGF. 1 hit.
    [Graphical view]
    SUPFAMiSSF57552. SSF57552. 1 hit.
    PROSITEiPS50215. ADAM_MEPRO. 1 hit.
    PS00427. DISINTEGRIN_1. 1 hit.
    PS50214. DISINTEGRIN_2. 1 hit.
    PS01186. EGF_2. 1 hit.
    PS50026. EGF_3. 1 hit.
    PS00142. ZINC_PROTEASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q61072-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGPRALSPLA SLRLRWLLAC GLLGPVLEAG RPDLEQTVHL SSYEIITPWR    50
    LTRERREALG PSSQQISYVI QAQGKQHIIH LERNTDLLPN DFVVYTYDKE 100
    GSLLSDHPNV QSHCHYRGYV EGVQNSAVAV SACFGLRGLL HLENASFGIE 150
    PLHNSSHFEH IFYPMDGIHQ EPLRCGVSNR DTEKEGTQGD EEEHPSVTQL 200
    LRRRRAVLPQ TRYVELFIVV DKERYDMMGR NQTAVREEMI RLANYLDSMY 250
    IMLNIRIVLV GLEIWTDRNP INIIGGAGDV LGNFVQWREK FLITRWRHDS 300
    AQLVLKKGFG GTAGMAFVGT VCSRSHAGGI NVFGQITVET FASIVAHELG 350
    HNLGMNHDDG RECFCGAKSC IMNSGASGSR NFSSCSAEDF EKLTLNKGGS 400
    CLLNIPKPDE AYSAPSCGNK LVDPGEECDC GTAKECEVDP CCEGSTCKLK 450
    SFAECAYGDC CKDCQFLPGG SMCRGKTSEC DVPEYCNGSS QFCPPDVFIQ 500
    NGYPCQNSKA YCYNGMCQYY DAQCQVIFGS KAKAAPRDCF IEVNSKGDRF 550
    GNCGFSGSEY KKCATGNALC GKLQCENVQD MPVFGIVPAI IQTPSRGTKC 600
    WGVDFQLGSD VPDPGMVNEG TKCDAGKICR NFQCVNASVL NYDCDIQGKC 650
    HGHGVCNSNK NCHCEDGWAP PHCDTKGYGG SVDSGPTYNA KSTALRDGLL 700
    VFFFLIVPLV AAAIFLFIKR DELRKTFRKK RSQMSDGRNQ ANVSRQPGDP 750
    SISRPPGGPN VSRPPGGPGV SRPPGGPGVS RPPGGPGVSR PPPGHGNRFP 800
    VPTYAAKQPA QFPSRPPPPQ PKISSQGNLI PARPAPAPPL YSSLT 845
    Length:845
    Mass (Da):92,079
    Last modified:July 27, 2011 - v2
    Checksum:i38C40F89D4A77725
    GO

    Sequence cautioni

    The sequence BAC65470.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti296 – 2961W → R in AAC52446. (PubMed:8647900)Curated
    Sequence conflicti296 – 2961W → R in BAC65470. (PubMed:12693553)Curated
    Sequence conflicti296 – 2961W → R in AAH47156. (PubMed:15489334)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U41765 mRNA. Translation: AAC52446.1.
    AK122188 mRNA. Translation: BAC65470.1. Different initiation.
    AC156553 Genomic DNA. No translation available.
    BC047156 mRNA. Translation: AAH47156.1.
    D50412 mRNA. Translation: BAA08913.1.
    U06145 mRNA. Translation: AAA18424.1.
    PIRiI48943.
    S60259.
    RefSeqiNP_031430.2. NM_007404.2.
    UniGeneiMm.28908.

    Genome annotation databases

    EnsembliENSMUST00000084035; ENSMUSP00000081048; ENSMUSG00000031555.
    GeneIDi11502.
    KEGGimmu:11502.
    UCSCiuc009lfk.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U41765 mRNA. Translation: AAC52446.1 .
    AK122188 mRNA. Translation: BAC65470.1 . Different initiation.
    AC156553 Genomic DNA. No translation available.
    BC047156 mRNA. Translation: AAH47156.1 .
    D50412 mRNA. Translation: BAA08913.1 .
    U06145 mRNA. Translation: AAA18424.1 .
    PIRi I48943.
    S60259.
    RefSeqi NP_031430.2. NM_007404.2.
    UniGenei Mm.28908.

    3D structure databases

    ProteinModelPortali Q61072.
    SMRi Q61072. Positions 212-638, 647-675.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q61072. 2 interactions.
    MINTi MINT-252904.

    Protein family/group databases

    MEROPSi M12.209.

    PTM databases

    PhosphoSitei Q61072.

    Proteomic databases

    PaxDbi Q61072.
    PRIDEi Q61072.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000084035 ; ENSMUSP00000081048 ; ENSMUSG00000031555 .
    GeneIDi 11502.
    KEGGi mmu:11502.
    UCSCi uc009lfk.2. mouse.

    Organism-specific databases

    CTDi 8754.
    MGIi MGI:105376. Adam9.
    Rougei Search...

    Phylogenomic databases

    eggNOGi NOG294463.
    GeneTreei ENSGT00750000117231.
    HOGENOMi HOG000230883.
    HOVERGENi HBG006978.
    InParanoidi Q61072.
    KOi K06834.
    OMAi FCQPDVF.
    OrthoDBi EOG7F7W89.
    TreeFami TF314733.

    Enzyme and pathway databases

    Reactomei REACT_199055. Collagen degradation.

    Miscellaneous databases

    NextBioi 278906.
    PROi Q61072.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q61072.
    Bgeei Q61072.
    Genevestigatori Q61072.

    Family and domain databases

    Gene3Di 3.40.390.10. 1 hit.
    4.10.70.10. 1 hit.
    InterProi IPR006586. ADAM_Cys-rich.
    IPR001762. Blood-coag_inhib_Disintegrin.
    IPR018358. Disintegrin_CS.
    IPR000742. EG-like_dom.
    IPR013032. EGF-like_CS.
    IPR024079. MetalloPept_cat_dom.
    IPR001590. Peptidase_M12B.
    IPR002870. Peptidase_M12B_N.
    [Graphical view ]
    Pfami PF08516. ADAM_CR. 1 hit.
    PF00200. Disintegrin. 1 hit.
    PF01562. Pep_M12B_propep. 1 hit.
    PF01421. Reprolysin. 1 hit.
    [Graphical view ]
    PRINTSi PR00289. DISINTEGRIN.
    SMARTi SM00608. ACR. 1 hit.
    SM00050. DISIN. 1 hit.
    SM00181. EGF. 1 hit.
    [Graphical view ]
    SUPFAMi SSF57552. SSF57552. 1 hit.
    PROSITEi PS50215. ADAM_MEPRO. 1 hit.
    PS00427. DISINTEGRIN_1. 1 hit.
    PS50214. DISINTEGRIN_2. 1 hit.
    PS01186. EGF_2. 1 hit.
    PS50026. EGF_3. 1 hit.
    PS00142. ZINC_PROTEASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "MDC9, a widely expressed cellular disintegrin containing cytoplasmic SH3 ligand domains."
      Weskamp G., Kraetzschmar J., Reid M.S., Blobel C.P.
      J. Cell Biol. 132:717-726(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Lung.
    2. "Prediction of the coding sequences of mouse homologues of KIAA gene: II. The complete nucleotide sequences of 400 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
      Okazaki N., Kikuno R., Ohara R., Inamoto S., Aizawa H., Yuasa S., Nakajima D., Nagase T., Ohara O., Koga H.
      DNA Res. 10:35-48(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: BrainImported.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: FVB/N-3.
      Tissue: Mammary gland.
    5. "A metalloprotease-disintegrin participating in myoblast fusion."
      Yagami-Hiromasa T., Sato T., Kurisaki T., Kamijo K., Nabeshima Y., Fujisawa-Sehara A.
      Nature 377:652-656(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 426-575.
    6. "A family of cellular proteins related to snake venom disintegrins."
      Weskamp G., Blobel C.P.
      Proc. Natl. Acad. Sci. U.S.A. 91:2748-2751(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 432-478.
      Strain: BALB/c.
    7. "Interaction of the metalloprotease disintegrins MDC9 and MDC15 with two SH3 domain-containing proteins, endophilin I and SH3PX1."
      Howard L., Nelson K.K., Maciewicz R.A., Blobel C.P.
      J. Biol. Chem. 274:31693-31699(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH SH3GL2 AND SNX9.

    Entry informationi

    Entry nameiADAM9_MOUSE
    AccessioniPrimary (citable) accession number: Q61072
    Secondary accession number(s): E9QPP2
    , Q60618, Q61853, Q80U94
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 19, 2004
    Last sequence update: July 27, 2011
    Last modified: October 1, 2014
    This is version 122 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. Peptidase families
      Classification of peptidase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3