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Q61072 (ADAM9_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 120. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Disintegrin and metalloproteinase domain-containing protein 9

Short name=ADAM 9
EC=3.4.24.-
Alternative name(s):
Meltrin-gamma
Metalloprotease/disintegrin/cysteine-rich protein 9
Myeloma cell metalloproteinase
Gene names
Name:Adam9
Synonyms:Kiaa0021, Mdc9, Mltng
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length845 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Probable zinc protease. May mediate cell-cell or cell-matrix interactions.

Cofactor

Binds 1 zinc ion per subunit Probable.

Subunit structure

Interacts with SH3GL2 and SNX9 through its cytoplasmic tail. Ref.7

Subcellular location

Membrane; Single-pass type I membrane protein Probable.

Sequence similarities

Contains 1 disintegrin domain.

Contains 1 EGF-like domain.

Contains 1 peptidase M12B domain.

Sequence caution

The sequence BAC65470.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Ontologies

Keywords
   Cellular componentMembrane
   DomainEGF-like domain
Signal
Transmembrane
Transmembrane helix
   LigandMetal-binding
Zinc
   Molecular functionHydrolase
Metalloprotease
Protease
   PTMDisulfide bond
Glycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processPMA-inducible membrane protein ectodomain proteolysis

Inferred from electronic annotation. Source: Ensembl

activation of MAPKK activity

Inferred from electronic annotation. Source: Ensembl

cell-cell adhesion mediated by integrin

Inferred from electronic annotation. Source: Ensembl

cell-matrix adhesion

Inferred from electronic annotation. Source: Ensembl

cellular response to lipopolysaccharide

Inferred from electronic annotation. Source: Ensembl

keratinocyte differentiation

Inferred from electronic annotation. Source: Ensembl

membrane protein ectodomain proteolysis

Inferred from direct assay PubMed 22480688. Source: BHF-UCL

monocyte activation

Inferred from electronic annotation. Source: Ensembl

positive regulation of cell adhesion mediated by integrin

Inferred from electronic annotation. Source: Ensembl

positive regulation of keratinocyte migration

Inferred from electronic annotation. Source: Ensembl

positive regulation of macrophage fusion

Inferred from electronic annotation. Source: Ensembl

positive regulation of membrane protein ectodomain proteolysis

Inferred from direct assay PubMed 9857183. Source: BHF-UCL

positive regulation of protein secretion

Inferred from electronic annotation. Source: Ensembl

response to calcium ion

Inferred from electronic annotation. Source: Ensembl

response to glucocorticoid

Inferred from mutant phenotype PubMed 16311053. Source: BHF-UCL

response to hydrogen peroxide

Inferred from electronic annotation. Source: Ensembl

response to laminar fluid shear stress

Inferred from electronic annotation. Source: Ensembl

response to manganese ion

Inferred from electronic annotation. Source: Ensembl

response to tumor necrosis factor

Inferred from electronic annotation. Source: Ensembl

transforming growth factor beta receptor signaling pathway

Inferred from mutant phenotype PubMed 11955914. Source: BHF-UCL

   Cellular_componentbasolateral plasma membrane

Inferred from electronic annotation. Source: Ensembl

cell surface

Inferred from direct assay Ref.1. Source: BHF-UCL

cytoplasm

Inferred from direct assay PubMed 15739225. Source: MGI

extracellular space

Inferred from mutant phenotype PubMed 9920899. Source: BHF-UCL

integral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

intrinsic component of external side of plasma membrane

Inferred from direct assay PubMed 9920899. Source: BHF-UCL

plasma membrane

Inferred from direct assay PubMed 15739225. Source: MGI

   Molecular_functionSH3 domain binding

Inferred from direct assay Ref.1. Source: BHF-UCL

metalloendopeptidase activity

Inferred from direct assay PubMed 22480688. Source: BHF-UCL

protein binding

Inferred from physical interaction Ref.7. Source: BHF-UCL

protein kinase C binding

Inferred from physical interaction PubMed 9857183. Source: BHF-UCL

zinc ion binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2929 Potential
Chain30 – 845816Disintegrin and metalloproteinase domain-containing protein 9
PRO_0000029063

Regions

Topological domain30 – 697668Extracellular Potential
Transmembrane698 – 71821Helical; Potential
Topological domain719 – 845127Cytoplasmic Potential
Domain212 – 406195Peptidase M12B
Domain414 – 50188Disintegrin
Domain644 – 69855EGF-like
Compositional bias505 – 634130Cys-rich

Sites

Active site3481 By similarity UniProtKB P78536
Metal binding3471Zinc; catalytic By similarity UniProtKB P78536
Metal binding3511Zinc; catalytic By similarity UniProtKB P78536
Metal binding3571Zinc; catalytic By similarity UniProtKB P78536

Amino acid modifications

Glycosylation1441N-linked (GlcNAc...) Potential
Glycosylation1541N-linked (GlcNAc...) Potential
Glycosylation2311N-linked (GlcNAc...) Potential
Glycosylation3811N-linked (GlcNAc...) Potential
Glycosylation4871N-linked (GlcNAc...) Potential
Glycosylation6361N-linked (GlcNAc...) Potential
Disulfide bond322 ↔ 401 By similarity
Disulfide bond363 ↔ 385 By similarity UniProtKB P78536
Disulfide bond365 ↔ 370 By similarity
Disulfide bond473 ↔ 493 By similarity
Disulfide bond644 ↔ 656 By similarity
Disulfide bond650 ↔ 662 By similarity
Disulfide bond664 ↔ 673 By similarity

Experimental info

Sequence conflict2961W → R in AAC52446. Ref.1
Sequence conflict2961W → R in BAC65470. Ref.2
Sequence conflict2961W → R in AAH47156. Ref.4

Sequences

Sequence LengthMass (Da)Tools
Q61072 [UniParc].

Last modified July 27, 2011. Version 2.
Checksum: 38C40F89D4A77725

FASTA84592,079
        10         20         30         40         50         60 
MGPRALSPLA SLRLRWLLAC GLLGPVLEAG RPDLEQTVHL SSYEIITPWR LTRERREALG 

        70         80         90        100        110        120 
PSSQQISYVI QAQGKQHIIH LERNTDLLPN DFVVYTYDKE GSLLSDHPNV QSHCHYRGYV 

       130        140        150        160        170        180 
EGVQNSAVAV SACFGLRGLL HLENASFGIE PLHNSSHFEH IFYPMDGIHQ EPLRCGVSNR 

       190        200        210        220        230        240 
DTEKEGTQGD EEEHPSVTQL LRRRRAVLPQ TRYVELFIVV DKERYDMMGR NQTAVREEMI 

       250        260        270        280        290        300 
RLANYLDSMY IMLNIRIVLV GLEIWTDRNP INIIGGAGDV LGNFVQWREK FLITRWRHDS 

       310        320        330        340        350        360 
AQLVLKKGFG GTAGMAFVGT VCSRSHAGGI NVFGQITVET FASIVAHELG HNLGMNHDDG 

       370        380        390        400        410        420 
RECFCGAKSC IMNSGASGSR NFSSCSAEDF EKLTLNKGGS CLLNIPKPDE AYSAPSCGNK 

       430        440        450        460        470        480 
LVDPGEECDC GTAKECEVDP CCEGSTCKLK SFAECAYGDC CKDCQFLPGG SMCRGKTSEC 

       490        500        510        520        530        540 
DVPEYCNGSS QFCPPDVFIQ NGYPCQNSKA YCYNGMCQYY DAQCQVIFGS KAKAAPRDCF 

       550        560        570        580        590        600 
IEVNSKGDRF GNCGFSGSEY KKCATGNALC GKLQCENVQD MPVFGIVPAI IQTPSRGTKC 

       610        620        630        640        650        660 
WGVDFQLGSD VPDPGMVNEG TKCDAGKICR NFQCVNASVL NYDCDIQGKC HGHGVCNSNK 

       670        680        690        700        710        720 
NCHCEDGWAP PHCDTKGYGG SVDSGPTYNA KSTALRDGLL VFFFLIVPLV AAAIFLFIKR 

       730        740        750        760        770        780 
DELRKTFRKK RSQMSDGRNQ ANVSRQPGDP SISRPPGGPN VSRPPGGPGV SRPPGGPGVS 

       790        800        810        820        830        840 
RPPGGPGVSR PPPGHGNRFP VPTYAAKQPA QFPSRPPPPQ PKISSQGNLI PARPAPAPPL 


YSSLT 

« Hide

References

« Hide 'large scale' references
[1]"MDC9, a widely expressed cellular disintegrin containing cytoplasmic SH3 ligand domains."
Weskamp G., Kraetzschmar J., Reid M.S., Blobel C.P.
J. Cell Biol. 132:717-726(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Lung.
[2]"Prediction of the coding sequences of mouse homologues of KIAA gene: II. The complete nucleotide sequences of 400 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
Okazaki N., Kikuno R., Ohara R., Inamoto S., Aizawa H., Yuasa S., Nakajima D., Nagase T., Ohara O., Koga H.
DNA Res. 10:35-48(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Brain.
[3]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: FVB/N-3.
Tissue: Mammary gland.
[5]"A metalloprotease-disintegrin participating in myoblast fusion."
Yagami-Hiromasa T., Sato T., Kurisaki T., Kamijo K., Nabeshima Y., Fujisawa-Sehara A.
Nature 377:652-656(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 426-575.
[6]"A family of cellular proteins related to snake venom disintegrins."
Weskamp G., Blobel C.P.
Proc. Natl. Acad. Sci. U.S.A. 91:2748-2751(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 432-478.
Strain: BALB/c.
[7]"Interaction of the metalloprotease disintegrins MDC9 and MDC15 with two SH3 domain-containing proteins, endophilin I and SH3PX1."
Howard L., Nelson K.K., Maciewicz R.A., Blobel C.P.
J. Biol. Chem. 274:31693-31699(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH SH3GL2 AND SNX9.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U41765 mRNA. Translation: AAC52446.1.
AK122188 mRNA. Translation: BAC65470.1. Different initiation.
AC156553 Genomic DNA. No translation available.
BC047156 mRNA. Translation: AAH47156.1.
D50412 mRNA. Translation: BAA08913.1.
U06145 mRNA. Translation: AAA18424.1.
PIRI48943.
S60259.
RefSeqNP_031430.2. NM_007404.2.
UniGeneMm.28908.

3D structure databases

ProteinModelPortalQ61072.
SMRQ61072. Positions 212-638, 647-675.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActQ61072. 2 interactions.
MINTMINT-252904.

Protein family/group databases

MEROPSM12.209.

PTM databases

PhosphoSiteQ61072.

Proteomic databases

PaxDbQ61072.
PRIDEQ61072.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000084035; ENSMUSP00000081048; ENSMUSG00000031555.
GeneID11502.
KEGGmmu:11502.
UCSCuc009lfk.2. mouse.

Organism-specific databases

CTD8754.
MGIMGI:105376. Adam9.
RougeSearch...

Phylogenomic databases

eggNOGNOG294463.
GeneTreeENSGT00750000117231.
HOGENOMHOG000230883.
HOVERGENHBG006978.
InParanoidQ61072.
KOK06834.
OMAFCQPDVF.
OrthoDBEOG7F7W89.
TreeFamTF314733.

Gene expression databases

ArrayExpressQ61072.
BgeeQ61072.
GenevestigatorQ61072.

Family and domain databases

Gene3D3.40.390.10. 1 hit.
4.10.70.10. 1 hit.
InterProIPR006586. ADAM_Cys-rich.
IPR001762. Blood-coag_inhib_Disintegrin.
IPR018358. Disintegrin_CS.
IPR000742. EG-like_dom.
IPR013032. EGF-like_CS.
IPR024079. MetalloPept_cat_dom.
IPR001590. Peptidase_M12B.
IPR002870. Peptidase_M12B_N.
[Graphical view]
PfamPF08516. ADAM_CR. 1 hit.
PF00200. Disintegrin. 1 hit.
PF01562. Pep_M12B_propep. 1 hit.
PF01421. Reprolysin. 1 hit.
[Graphical view]
PRINTSPR00289. DISINTEGRIN.
SMARTSM00608. ACR. 1 hit.
SM00050. DISIN. 1 hit.
SM00181. EGF. 1 hit.
[Graphical view]
SUPFAMSSF57552. SSF57552. 1 hit.
PROSITEPS50215. ADAM_MEPRO. 1 hit.
PS00427. DISINTEGRIN_1. 1 hit.
PS50214. DISINTEGRIN_2. 1 hit.
PS01186. EGF_2. 1 hit.
PS50026. EGF_3. 1 hit.
PS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio278906.
PROQ61072.
SOURCESearch...

Entry information

Entry nameADAM9_MOUSE
AccessionPrimary (citable) accession number: Q61072
Secondary accession number(s): E9QPP2 expand/collapse secondary AC list , Q60618, Q61853, Q80U94
Entry history
Integrated into UniProtKB/Swiss-Prot: July 19, 2004
Last sequence update: July 27, 2011
Last modified: June 11, 2014
This is version 120 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot