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Protein

Etoposide-induced protein 2.4

Gene

Ei24

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Acts as a negative growth regulator via p53-mediated apoptosis pathway. Regulates formation of degradative autolysosomes during autophagy (By similarity).By similarity2 Publications

GO - Biological processi

  • autophagy Source: MGI
  • cellular response to UV-C Source: MGI
  • intrinsic apoptotic signaling pathway in response to DNA damage Source: MGI
  • macroautophagy Source: GO_Central
  • negative regulation of cell growth Source: MGI
  • negative regulation of protein import into nucleus Source: MGI
  • neuromuscular process controlling balance Source: MGI
  • positive regulation of intrinsic apoptotic signaling pathway Source: MGI
  • response to drug Source: MGI
Complete GO annotation...

Keywords - Biological processi

Apoptosis, Autophagy

Names & Taxonomyi

Protein namesi
Recommended name:
Etoposide-induced protein 2.4
Alternative name(s):
p53-induced gene 8 protein
Gene namesi
Name:Ei24
Synonyms:Pig8
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:108090. Ei24.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei77 – 97HelicalSequence analysisAdd BLAST21
Transmembranei117 – 137HelicalSequence analysisAdd BLAST21
Transmembranei179 – 199HelicalSequence analysisAdd BLAST21
Transmembranei238 – 255HelicalSequence analysisAdd BLAST18
Transmembranei257 – 277HelicalSequence analysisAdd BLAST21

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Endoplasmic reticulum, Membrane, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00000869462 – 340Etoposide-induced protein 2.4Add BLAST339

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Modified residuei46PhosphoserineBy similarity1
Modified residuei47PhosphoserineBy similarity1
Modified residuei56PhosphoserineCombined sources1
Modified residuei320PhosphoserineCombined sources1
Modified residuei326PhosphoserineCombined sources1
Modified residuei330PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ61070.
MaxQBiQ61070.
PaxDbiQ61070.
PeptideAtlasiQ61070.
PRIDEiQ61070.

PTM databases

iPTMnetiQ61070.
PhosphoSitePlusiQ61070.

Expressioni

Tissue specificityi

Found in all the examined tissues. High expression was found in liver, skeletal muscle, pancreas, kidney heart and to a lesser extent in brain, placenta and lung.

Inductioni

By etoposide treatment, induction requires p53. Etoposide induces DNA damage in cells by inhibiting DNA topoisomerase II, and ultimately causes apoptotic cell death.2 Publications

Gene expression databases

BgeeiENSMUSG00000062762.
CleanExiMM_EI24.

Interactioni

Subunit structurei

Interacts with BCL2.1 Publication

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000110738.

Structurei

3D structure databases

ProteinModelPortaliQ61070.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni52 – 115Interaction with BH3 domain of BCL2Add BLAST64

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi41 – 44Poly-Arg4
Compositional biasi316 – 320Poly-Ser5

Sequence similaritiesi

Belongs to the EI24 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3966. Eukaryota.
ENOG410XRPU. LUCA.
HOGENOMiHOG000293197.
HOVERGENiHBG001857.
InParanoidiQ61070.
KOiK10134.
PhylomeDBiQ61070.
TreeFamiTF314441.

Family and domain databases

InterProiIPR009890. EI24.
[Graphical view]
PANTHERiPTHR21389. PTHR21389. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q61070-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MADSVKTFLQ DLGRGIKDSI WGICTISKLD ARIQQKREEQ RRRRASSLLA
60 70 80 90 100
QRRPQSVERK QESEPRIVSR IFQCCAWNGG VFWFSLLLFY RVFIPVLQSV
110 120 130 140 150
TARIIGDPSL HGDVWSWLEF FLTSIFSALW VLPLFVLSKV VNAIWFQDIA
160 170 180 190 200
DLAFEVSGRK PHPFPSVSKI IADMLFNLLL QALFLIQGMF VSLFPIHLVG
210 220 230 240 250
QLVSLLHMSL LYSLYCFEYR WFNKGIEMHQ RLSNIERNWP YYFGFGLPLA
260 270 280 290 300
FLTAMQSSYI ISGCLFSILF PLFIISANEA KTPGKAYLFQ LRLFSLVVFL
310 320 330 340
SNRLFHKTVY LQSALSSSSS AEKFPSPHPS PAKLKAAAGH
Length:340
Mass (Da):38,933
Last modified:May 5, 2009 - v3
Checksum:iAA3B824B2CE330BC
GO

Sequence cautioni

The sequence AAC52483 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
The sequence BAE28130 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
The sequence BAE35489 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
The sequence BAE42899 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti78N → D in BAE28130 (PubMed:16141072).Curated1
Sequence conflicti144I → F in BAE35489 (PubMed:16141072).Curated1
Sequence conflicti320S → P in BAE42899 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U41751 mRNA. Translation: AAC52483.2. Different initiation.
AK147776 mRNA. Translation: BAE28130.1. Different initiation.
AK159927 mRNA. Translation: BAE35489.1. Different initiation.
AK172233 mRNA. Translation: BAE42899.1. Different initiation.
RefSeqiNP_001186423.1. NM_001199494.1.
NP_031941.1. NM_007915.5.
UniGeneiMm.4337.

Genome annotation databases

GeneIDi13663.
KEGGimmu:13663.
UCSCiuc009oua.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U41751 mRNA. Translation: AAC52483.2. Different initiation.
AK147776 mRNA. Translation: BAE28130.1. Different initiation.
AK159927 mRNA. Translation: BAE35489.1. Different initiation.
AK172233 mRNA. Translation: BAE42899.1. Different initiation.
RefSeqiNP_001186423.1. NM_001199494.1.
NP_031941.1. NM_007915.5.
UniGeneiMm.4337.

3D structure databases

ProteinModelPortaliQ61070.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000110738.

PTM databases

iPTMnetiQ61070.
PhosphoSitePlusiQ61070.

Proteomic databases

EPDiQ61070.
MaxQBiQ61070.
PaxDbiQ61070.
PeptideAtlasiQ61070.
PRIDEiQ61070.

Protocols and materials databases

DNASUi13663.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi13663.
KEGGimmu:13663.
UCSCiuc009oua.2. mouse.

Organism-specific databases

CTDi9538.
MGIiMGI:108090. Ei24.

Phylogenomic databases

eggNOGiKOG3966. Eukaryota.
ENOG410XRPU. LUCA.
HOGENOMiHOG000293197.
HOVERGENiHBG001857.
InParanoidiQ61070.
KOiK10134.
PhylomeDBiQ61070.
TreeFamiTF314441.

Miscellaneous databases

ChiTaRSiEi24. mouse.
PROiQ61070.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000062762.
CleanExiMM_EI24.

Family and domain databases

InterProiIPR009890. EI24.
[Graphical view]
PANTHERiPTHR21389. PTHR21389. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiEI24_MOUSE
AccessioniPrimary (citable) accession number: Q61070
Secondary accession number(s): Q3T9X1, Q3TVX9, Q3UGS7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: May 5, 2009
Last modified: November 2, 2016
This is version 116 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.