Q61068 (UBPW_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ubiquitin carboxyl-terminal hydrolase DUB-1
EC=3.4.19.12

Alternative name(s):
Deubiquitinating enzyme 1
Ubiquitin thioesterase DUB-1
Ubiquitin-specific-processing protease DUB-1

Gene names

Name:	Dub1
Synonyms:	Dub-1

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	526 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Has growth-suppressing activity, induces arrest in G1 phase upon controlled expression.
Catalytic activity	Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
Induction	By interleukin-3.
Sequence similarities	Belongs to the peptidase C19 family.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Molecular function	Hydrolase Protease Thiol protease
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	protein deubiquitination Inferred from direct assay Ref.1. Source: MGI ubiquitin-dependent protein catabolic process Inferred from electronic annotation. Source: InterPro
Molecular_function	ubiquitin thiolesterase activity Inferred from electronic annotation. Source: InterPro ubiquitin-specific protease activity Inferred from direct assay Ref.1. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 526	526	Ubiquitin carboxyl-terminal hydrolase DUB-1		PRO_0000080683
Sites
	Active site	60	1	Nucleophile
	Active site	307	1	Proton acceptor By similarity
Experimental info
	Mutagenesis	60	1	C → S: Loss of activity. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q61068 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 263AA7B7579694EA	FASTA	526	59,073
	10 20 30 40 50 60 MVVALSFPEA DPALSSPDAP ELHQDEAQVV EELTVNGKHS LSWESPQGPG CGLQNTGNSC 70 80 90 100 110 120 YLNAALQCLT HTPPLADYML SQEHSQTCCS PEGCKLCAME ALVTQSLLHS HSGDVMKPSH 130 140 150 160 170 180 ILTSAFHKHQ QEDAHEFLMF TLETMHESCL QVHRQSKPTS EDSSPIHDIF GGWWRSQIKC 190 200 210 220 230 240 LLCQGTSDTY DRFLDIPLDI SSAQSVKQAL WDTEKSEELC GDNAYYCGKC RQKMPASKTL 250 260 270 280 290 300 HVHIAPKVLM VVLNRFSAFT GNKLDRKVSY PEFLDLKPYL SEPTGGPLPY ALYAVLVHDG 310 320 330 340 350 360 ATSHSGHYFC CVKAGHGKWY KMDDTKVTRC DVTSVLNENA YVLFYVQQAN LKQVSIDMPE 370 380 390 400 410 420 GRINEVLDPE YQLKKSRRKK HKKKSPFTED LGEPCENRDK RAIKETSLGK GKVLQEVNHK 430 440 450 460 470 480 KAGQKHGNTK LMPQKQNHQK AGQNLRNTEV ELDLPADAIV IHQPRSTANW GRDSPDKENQ 490 500 510 520 PLHNADRLLT SQGPVNTWQL CRQEGRRRSK KGQNKNKQGQ RLLLVC

References

[1]	"DUB-1, a deubiquitinating enzyme with growth-suppressing activity." Zhu Y., Carroll M., Papa F.R., Hochstrasser M., D'Andrea A.D. Proc. Natl. Acad. Sci. U.S.A. 93:3275-3279(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], MUTAGENESIS OF CYS-60.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U41636 mRNA. Translation: AAC52532.1.
IPI	IPI00117061.
PIR	JC6133.
RefSeq	NP_031913.1. NM_007887.2.
UniGene	Mm.389929.
3D structure databases
ProteinModelPortal	Q61068.
SMR	Q61068. Positions 52-345.
ModBase	Search...
Protein family/group databases
MEROPS	C19.031.
Proteomic databases
PRIDE	Q61068.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000067695; ENSMUSP00000068997; ENSMUSG00000054568.
GeneID	13531.
KEGG	mmu:13531.
UCSC	uc009iwz.2. mouse.
Organism-specific databases
CTD	13531.
MGI	MGI:107699. Dub1.
Phylogenomic databases
eggNOG	COG5533.
GeneTree	ENSGT00670000097591.
HOGENOM	HOG000144638.
HOVERGEN	HBG007129.
InParanoid	Q61068.
KO	K11845.
Gene expression databases
Bgee	Q61068.
CleanEx	MM_DUB1.
Genevestigator	Q61068.
GermOnline	ENSMUSG00000054568. Mus musculus.
Family and domain databases
InterPro	IPR018200. Pept_C19ubi-hydrolase_C_CS. IPR001394. Peptidase_C19. [Graphical view]
Pfam	PF00443. UCH. 1 hit. [Graphical view]
PROSITE	PS00972. UCH_2_1. 1 hit. PS00973. UCH_2_2. 1 hit. PS50235. UCH_2_3. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	284126.
SOURCE	Search...

Entry information

Entry name

UBPW_MOUSE

Accession

Primary (citable) accession number: Q61068

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	May 1, 2013
This is version 94 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program