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Protein

Nuclear receptor subfamily 0 group B member 1

Gene

Nr0b1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Orphan nuclear receptor. Component of a cascade required for the development of the hypothalamic-pituitary-adrenal-gonadal axis. Acts as a coregulatory protein that inhibits the transcriptional activity of other nuclear receptors through heterodimeric interactions. May also have a role in the development of the embryo and in the maintenance of embryonic stem cell pluripotency (By similarity).By similarity

GO - Molecular functioni

  1. AF-2 domain binding Source: MGI
  2. DNA binding Source: MGI
  3. DNA hairpin binding Source: MGI
  4. ligand-activated sequence-specific DNA binding RNA polymerase II transcription factor activity Source: MGI
  5. protein domain specific binding Source: MGI
  6. protein homodimerization activity Source: MGI
  7. RNA binding Source: HGNC
  8. sequence-specific DNA binding Source: MGI
  9. steroid hormone receptor activity Source: InterPro
  10. steroid hormone receptor binding Source: MGI
  11. transcription corepressor activity Source: MGI
  12. transcription factor binding Source: MGI

GO - Biological processi

  1. adrenal gland development Source: MGI
  2. cell differentiation Source: MGI
  3. gonad development Source: MGI
  4. intracellular receptor signaling pathway Source: MGI
  5. Leydig cell differentiation Source: MGI
  6. male gonad development Source: MGI
  7. male sex determination Source: MGI
  8. negative regulation of cell differentiation Source: MGI
  9. negative regulation of intracellular steroid hormone receptor signaling pathway Source: MGI
  10. negative regulation of sequence-specific DNA binding transcription factor activity Source: MGI
  11. negative regulation of transcription, DNA-templated Source: MGI
  12. negative regulation of transcription from RNA polymerase II promoter Source: MGI
  13. protein localization Source: MGI
  14. response to immobilization stress Source: MGI
  15. Sertoli cell differentiation Source: MGI
  16. sex determination Source: MGI
  17. spermatogenesis Source: MGI
  18. steroid biosynthetic process Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Receptor, Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Enzyme and pathway databases

ReactomeiREACT_276696. Nuclear Receptor transcription pathway.

Names & Taxonomyi

Protein namesi
Recommended name:
Nuclear receptor subfamily 0 group B member 1
Alternative name(s):
Nuclear receptor DAX-1
Gene namesi
Name:Nr0b1
Synonyms:Ahch, Dax1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome X

Organism-specific databases

MGIiMGI:1352460. Nr0b1.

Subcellular locationi

Nucleus By similarity. Cytoplasm By similarity
Note: Shuttles between the cytoplasm and nucleus. Homodimers exits in the cytoplasm and in the nucleus (By similarity).By similarity

GO - Cellular componenti

  1. cytoplasm Source: MGI
  2. membrane Source: MGI
  3. nucleus Source: HGNC
  4. polysomal ribosome Source: HGNC
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 472472Nuclear receptor subfamily 0 group B member 1PRO_0000053749Add
BLAST

Proteomic databases

PRIDEiQ61066.

PTM databases

PhosphoSiteiQ61066.

Expressioni

Tissue specificityi

Expressed in adult cerebral cortex, spinal cord, thymus, heart, lung, ovary, testis, adrenal gland, hypothalamus, spleen and kidney.1 Publication

Developmental stagei

Expressed in E5.5 throughout the embryo except in the proximal visceral endoderm.1 Publication

Gene expression databases

BgeeiQ61066.
CleanExiMM_NR0B1.
ExpressionAtlasiQ61066. baseline and differential.
GenevestigatoriQ61066.

Interactioni

Subunit structurei

Homodimer. Interacts with NR5A1, NR5A2, NR0B2 and with COPS2 (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
EsrrbQ615392EBI-2312665,EBI-2312731
NanogQ80Z647EBI-2312665,EBI-2312517
Pou5f1P202635EBI-2312665,EBI-1606219
Rif1Q6PR542EBI-2312665,EBI-2312621
Slc8a1P704142EBI-2312665,EBI-2312694

Protein-protein interaction databases

BioGridi198035. 17 interactions.
DIPiDIP-29929N.
IntActiQ61066. 13 interactions.

Structurei

Secondary structure

1
472
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi253 – 27220Combined sources
Helixi275 – 2784Combined sources
Helixi282 – 30322Combined sources
Helixi359 – 37214Combined sources
Helixi377 – 38812Combined sources
Helixi399 – 42022Combined sources
Helixi426 – 43813Combined sources
Helixi445 – 4506Combined sources
Helixi452 – 4554Combined sources
Helixi460 – 4678Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3F5CX-ray3.00B/C205-472[»]
ProteinModelPortaliQ61066.
SMRiQ61066. Positions 251-472.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ61066.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati1 – 67671Add
BLAST
Repeati68 – 135682Add
BLAST
Repeati136 – 202673Add
BLAST
Repeati203 – 255534; truncatedAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 2552554 X 67 AA tandem repeatsAdd
BLAST
Regioni256 – 472217Ligand-bindingBy similarityAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi13 – 175LXXLL motif 1
Motifi80 – 845LXXLL motif 2
Motifi148 – 1525LXXLL motif 3
Motifi463 – 4686AF-2 motif

Domaini

Homodimerization involved an interaction between amino and carboxy termini involving LXXLL motifs and steroid binding domain (AF-2 motif). Heterodimerizes with NR5A1 and NROB2 through its N-terminal LXXLL motifs (By similarity).By similarity

Sequence similaritiesi

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiNOG238310.
GeneTreeiENSGT00390000015719.
HOGENOMiHOG000231151.
HOVERGENiHBG005453.
InParanoidiQ61066.
KOiK08562.
OMAiCCFCGED.
OrthoDBiEOG7NSB35.
PhylomeDBiQ61066.
TreeFamiTF332386.

Family and domain databases

Gene3Di1.10.565.10. 1 hit.
InterProiIPR008946. Nucl_hormone_rcpt_ligand-bd.
IPR000536. Nucl_hrmn_rcpt_lig-bd_core.
IPR025900. Nuclear_receptor_repeat.
IPR001723. Str_hrmn_rcpt.
[Graphical view]
PfamiPF00104. Hormone_recep. 1 hit.
PF14046. NR_Repeat. 4 hits.
[Graphical view]
PRINTSiPR00398. STRDHORMONER.
SMARTiSM00430. HOLI. 1 hit.
[Graphical view]
SUPFAMiSSF48508. SSF48508. 1 hit.

Sequencei

Sequence statusi: Complete.

Q61066-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGEDHPWQG SILYNLLMSA KQKHASQEER EVRLGAQCWG CACGAQPVLG
60 70 80 90 100
GERLSGGQAR SLLYRCCFCG ENHPRQGGIL YSMLTNARQP SVATQAPRAR
110 120 130 140 150
FGAPCWGCAC GSAEPLVGRE GLPAGQAPSL LYRCCFCGEE HPRQGSILYS
160 170 180 190 200
LLTSAQQTHV SREAPEAHRR GEWWQLSYCT QSVGGPEGLQ STQAMAFLYR
210 220 230 240 250
SYVCGEEQPQ QISVASGTPV SADQTPATPQ EQPRAPWWDA SPGVQRLITL
260 270 280 290 300
KDPQVVCEAA SAGLLKTLRF VKYLPCFQIL PLDQQLVLVR SCWAPLLMLE
310 320 330 340 350
LAQDHLHFEM MEIPETNTTQ EMLTTRRQET EGPEPAEPQA TEQPQMVSAE
360 370 380 390 400
AGHLLPAAAV QAIKSFFFKC WSLNIDTKEY AYLKGTVLFN PDLPGLQCVK
410 420 430 440 450
YIEGLQWRTQ QILTEHIRMM QREYQIRSAE LNSALFLLRF INSDVVTELF
460 470
FRPIIGAVSM DDMMLEMLCA KL
Length:472
Mass (Da):52,576
Last modified:November 1, 1996 - v1
Checksum:iDE1FB01C30F883CF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S83180, S83178 Genomic DNA. Translation: AAB46875.1.
U41568 mRNA. Translation: AAC52920.1.
CCDSiCCDS30260.1.
PIRiJC5199.
RefSeqiNP_031456.1. NM_007430.4.
UniGeneiMm.5180.

Genome annotation databases

EnsembliENSMUST00000026036; ENSMUSP00000026036; ENSMUSG00000025056.
GeneIDi11614.
KEGGimmu:11614.
UCSCiuc009tsd.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S83180, S83178 Genomic DNA. Translation: AAB46875.1.
U41568 mRNA. Translation: AAC52920.1.
CCDSiCCDS30260.1.
PIRiJC5199.
RefSeqiNP_031456.1. NM_007430.4.
UniGeneiMm.5180.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3F5CX-ray3.00B/C205-472[»]
ProteinModelPortaliQ61066.
SMRiQ61066. Positions 251-472.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi198035. 17 interactions.
DIPiDIP-29929N.
IntActiQ61066. 13 interactions.

PTM databases

PhosphoSiteiQ61066.

Proteomic databases

PRIDEiQ61066.

Protocols and materials databases

DNASUi11614.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000026036; ENSMUSP00000026036; ENSMUSG00000025056.
GeneIDi11614.
KEGGimmu:11614.
UCSCiuc009tsd.1. mouse.

Organism-specific databases

CTDi190.
MGIiMGI:1352460. Nr0b1.

Phylogenomic databases

eggNOGiNOG238310.
GeneTreeiENSGT00390000015719.
HOGENOMiHOG000231151.
HOVERGENiHBG005453.
InParanoidiQ61066.
KOiK08562.
OMAiCCFCGED.
OrthoDBiEOG7NSB35.
PhylomeDBiQ61066.
TreeFamiTF332386.

Enzyme and pathway databases

ReactomeiREACT_276696. Nuclear Receptor transcription pathway.

Miscellaneous databases

EvolutionaryTraceiQ61066.
NextBioi279166.
PROiQ61066.
SOURCEiSearch...

Gene expression databases

BgeeiQ61066.
CleanExiMM_NR0B1.
ExpressionAtlasiQ61066. baseline and differential.
GenevestigatoriQ61066.

Family and domain databases

Gene3Di1.10.565.10. 1 hit.
InterProiIPR008946. Nucl_hormone_rcpt_ligand-bd.
IPR000536. Nucl_hrmn_rcpt_lig-bd_core.
IPR025900. Nuclear_receptor_repeat.
IPR001723. Str_hrmn_rcpt.
[Graphical view]
PfamiPF00104. Hormone_recep. 1 hit.
PF14046. NR_Repeat. 4 hits.
[Graphical view]
PRINTSiPR00398. STRDHORMONER.
SMARTiSM00430. HOLI. 1 hit.
[Graphical view]
SUPFAMiSSF48508. SSF48508. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Ahch, the mouse homologue of DAX1: cloning, characterization and synteny with GyK, the glycerol kinase locus."
    Guo W., Lovell R.S., Zhang Y.H., Huang B.L., Burris T.P., Craigen W.J., McCabe E.R.B.
    Gene 178:31-34(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: CD-1.
    Tissue: Testis.
  2. "Characterization of the mouse DAX-1 gene reveals evolutionary conservation of a unique amino-terminal motif and widespread expression in mouse tissue."
    Bae D.S., Schaefer M.L., Partan B.W., Muglia L.
    Endocrinology 137:3921-3927(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], TISSUE SPECIFICITY.
  3. "Novel role for the orphan nuclear receptor Dax1 in embryogenesis, different from steroidogenesis."
    Niakan K.K., Davis E.C., Clipsham R.C., Jiang M., Dehart D.B., Sulik K.K., McCabe E.R.B.
    Mol. Genet. Metab. 88:261-271(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: DEVELOPMENTAL STAGE, FUNCTION.

Entry informationi

Entry nameiNR0B1_MOUSE
AccessioniPrimary (citable) accession number: Q61066
Secondary accession number(s): O09068, O09147
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 1, 1996
Last modified: April 1, 2015
This is version 133 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.