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Protein

Nuclear receptor subfamily 0 group B member 1

Gene

Nr0b1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Orphan nuclear receptor. Component of a cascade required for the development of the hypothalamic-pituitary-adrenal-gonadal axis. Acts as a coregulatory protein that inhibits the transcriptional activity of other nuclear receptors through heterodimeric interactions. May also have a role in the development of the embryo and in the maintenance of embryonic stem cell pluripotency (By similarity).By similarity1 Publication

GO - Molecular functioni

GO - Biological processi

  • adrenal gland development Source: MGI
  • cell differentiation Source: MGI
  • gonad development Source: MGI
  • Leydig cell differentiation Source: MGI
  • male gonad development Source: MGI
  • male sex determination Source: MGI
  • negative regulation of cell differentiation Source: MGI
  • negative regulation of intracellular steroid hormone receptor signaling pathway Source: MGI
  • negative regulation of sequence-specific DNA binding transcription factor activity Source: MGI
  • negative regulation of transcription, DNA-templated Source: MGI
  • negative regulation of transcription from RNA polymerase II promoter Source: MGI
  • protein localization Source: MGI
  • response to immobilization stress Source: MGI
  • Sertoli cell differentiation Source: MGI
  • sex determination Source: MGI
  • spermatogenesis Source: MGI
  • steroid biosynthetic process Source: MGI
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Receptor, Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-MMU-383280. Nuclear Receptor transcription pathway.

Names & Taxonomyi

Protein namesi
Recommended name:
Nuclear receptor subfamily 0 group B member 1
Alternative name(s):
Nuclear receptor DAX-1
Gene namesi
Name:Nr0b1
Synonyms:Ahch, Dax1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome X

Organism-specific databases

MGIiMGI:1352460. Nr0b1.

Subcellular locationi

  • Nucleus By similarity
  • Cytoplasm By similarity

  • Note: Shuttles between the cytoplasm and nucleus. Homodimers exits in the cytoplasm and in the nucleus (By similarity).By similarity

GO - Cellular componenti

  • cytoplasm Source: MGI
  • membrane Source: MGI
  • nucleoplasm Source: MGI
  • nucleus Source: HGNC
  • polysomal ribosome Source: HGNC
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000537491 – 472Nuclear receptor subfamily 0 group B member 1Add BLAST472

Proteomic databases

PaxDbiQ61066.
PRIDEiQ61066.

PTM databases

iPTMnetiQ61066.
PhosphoSitePlusiQ61066.

Expressioni

Tissue specificityi

Expressed in adult cerebral cortex, spinal cord, thymus, heart, lung, ovary, testis, adrenal gland, hypothalamus, spleen and kidney.1 Publication

Developmental stagei

Expressed in E5.5 throughout the embryo except in the proximal visceral endoderm.1 Publication

Gene expression databases

BgeeiENSMUSG00000025056.
CleanExiMM_NR0B1.
ExpressionAtlasiQ61066. baseline and differential.
GenevisibleiQ61066. MM.

Interactioni

Subunit structurei

Homodimer. Interacts with NR5A1, NR5A2, NR0B2 and with COPS2 (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
EsrrbQ615392EBI-2312665,EBI-2312731
NanogQ80Z647EBI-2312665,EBI-2312517
Pou5f1P202635EBI-2312665,EBI-1606219
Rif1Q6PR542EBI-2312665,EBI-2312621
Slc8a1P704142EBI-2312665,EBI-2312694

GO - Molecular functioni

Protein-protein interaction databases

BioGridi198035. 17 interactors.
DIPiDIP-29929N.
IntActiQ61066. 13 interactors.
STRINGi10090.ENSMUSP00000026036.

Structurei

Secondary structure

1472
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi253 – 272Combined sources20
Helixi275 – 278Combined sources4
Helixi282 – 303Combined sources22
Helixi359 – 372Combined sources14
Helixi377 – 388Combined sources12
Helixi399 – 420Combined sources22
Helixi426 – 438Combined sources13
Helixi445 – 450Combined sources6
Helixi452 – 455Combined sources4
Helixi460 – 467Combined sources8

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3F5CX-ray3.00B/C205-472[»]
ProteinModelPortaliQ61066.
SMRiQ61066.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ61066.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati1 – 671Add BLAST67
Repeati68 – 1352Add BLAST68
Repeati136 – 2023Add BLAST67
Repeati203 – 2554; truncatedAdd BLAST53

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 2554 X 67 AA tandem repeatsAdd BLAST255
Regioni256 – 472Ligand-bindingBy similarityAdd BLAST217

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi13 – 17LXXLL motif 15
Motifi80 – 84LXXLL motif 25
Motifi148 – 152LXXLL motif 35
Motifi463 – 468AF-2 motif6

Domaini

Homodimerization involved an interaction between amino and carboxy termini involving LXXLL motifs and steroid binding domain (AF-2 motif). Heterodimerizes with NR5A1 and NROB2 through its N-terminal LXXLL motifs (By similarity).By similarity

Sequence similaritiesi

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG3575. Eukaryota.
ENOG410XRZC. LUCA.
GeneTreeiENSGT00390000015719.
HOGENOMiHOG000231151.
HOVERGENiHBG005453.
InParanoidiQ61066.
KOiK08562.
OMAiGPCWGCS.
OrthoDBiEOG091G0FGK.
PhylomeDBiQ61066.
TreeFamiTF332386.

Family and domain databases

Gene3Di1.10.565.10. 1 hit.
InterProiIPR033544. NR0B1.
IPR000536. Nucl_hrmn_rcpt_lig-bd.
IPR001723. Nuclear_hrmn_rcpt.
IPR025900. Nuclear_receptor_repeat.
[Graphical view]
PANTHERiPTHR24081:SF1. PTHR24081:SF1. 3 hits.
PfamiPF00104. Hormone_recep. 1 hit.
PF14046. NR_Repeat. 4 hits.
[Graphical view]
PRINTSiPR00398. STRDHORMONER.
SMARTiSM00430. HOLI. 1 hit.
[Graphical view]
SUPFAMiSSF48508. SSF48508. 1 hit.

Sequencei

Sequence statusi: Complete.

Q61066-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGEDHPWQG SILYNLLMSA KQKHASQEER EVRLGAQCWG CACGAQPVLG
60 70 80 90 100
GERLSGGQAR SLLYRCCFCG ENHPRQGGIL YSMLTNARQP SVATQAPRAR
110 120 130 140 150
FGAPCWGCAC GSAEPLVGRE GLPAGQAPSL LYRCCFCGEE HPRQGSILYS
160 170 180 190 200
LLTSAQQTHV SREAPEAHRR GEWWQLSYCT QSVGGPEGLQ STQAMAFLYR
210 220 230 240 250
SYVCGEEQPQ QISVASGTPV SADQTPATPQ EQPRAPWWDA SPGVQRLITL
260 270 280 290 300
KDPQVVCEAA SAGLLKTLRF VKYLPCFQIL PLDQQLVLVR SCWAPLLMLE
310 320 330 340 350
LAQDHLHFEM MEIPETNTTQ EMLTTRRQET EGPEPAEPQA TEQPQMVSAE
360 370 380 390 400
AGHLLPAAAV QAIKSFFFKC WSLNIDTKEY AYLKGTVLFN PDLPGLQCVK
410 420 430 440 450
YIEGLQWRTQ QILTEHIRMM QREYQIRSAE LNSALFLLRF INSDVVTELF
460 470
FRPIIGAVSM DDMMLEMLCA KL
Length:472
Mass (Da):52,576
Last modified:November 1, 1996 - v1
Checksum:iDE1FB01C30F883CF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S83180, S83178 Genomic DNA. Translation: AAB46875.1.
U41568 mRNA. Translation: AAC52920.1.
CCDSiCCDS30260.1.
PIRiJC5199.
RefSeqiNP_031456.1. NM_007430.5.
UniGeneiMm.5180.

Genome annotation databases

EnsembliENSMUST00000026036; ENSMUSP00000026036; ENSMUSG00000025056.
GeneIDi11614.
KEGGimmu:11614.
UCSCiuc009tsd.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S83180, S83178 Genomic DNA. Translation: AAB46875.1.
U41568 mRNA. Translation: AAC52920.1.
CCDSiCCDS30260.1.
PIRiJC5199.
RefSeqiNP_031456.1. NM_007430.5.
UniGeneiMm.5180.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3F5CX-ray3.00B/C205-472[»]
ProteinModelPortaliQ61066.
SMRiQ61066.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi198035. 17 interactors.
DIPiDIP-29929N.
IntActiQ61066. 13 interactors.
STRINGi10090.ENSMUSP00000026036.

PTM databases

iPTMnetiQ61066.
PhosphoSitePlusiQ61066.

Proteomic databases

PaxDbiQ61066.
PRIDEiQ61066.

Protocols and materials databases

DNASUi11614.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000026036; ENSMUSP00000026036; ENSMUSG00000025056.
GeneIDi11614.
KEGGimmu:11614.
UCSCiuc009tsd.1. mouse.

Organism-specific databases

CTDi190.
MGIiMGI:1352460. Nr0b1.

Phylogenomic databases

eggNOGiKOG3575. Eukaryota.
ENOG410XRZC. LUCA.
GeneTreeiENSGT00390000015719.
HOGENOMiHOG000231151.
HOVERGENiHBG005453.
InParanoidiQ61066.
KOiK08562.
OMAiGPCWGCS.
OrthoDBiEOG091G0FGK.
PhylomeDBiQ61066.
TreeFamiTF332386.

Enzyme and pathway databases

ReactomeiR-MMU-383280. Nuclear Receptor transcription pathway.

Miscellaneous databases

EvolutionaryTraceiQ61066.
PROiQ61066.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000025056.
CleanExiMM_NR0B1.
ExpressionAtlasiQ61066. baseline and differential.
GenevisibleiQ61066. MM.

Family and domain databases

Gene3Di1.10.565.10. 1 hit.
InterProiIPR033544. NR0B1.
IPR000536. Nucl_hrmn_rcpt_lig-bd.
IPR001723. Nuclear_hrmn_rcpt.
IPR025900. Nuclear_receptor_repeat.
[Graphical view]
PANTHERiPTHR24081:SF1. PTHR24081:SF1. 3 hits.
PfamiPF00104. Hormone_recep. 1 hit.
PF14046. NR_Repeat. 4 hits.
[Graphical view]
PRINTSiPR00398. STRDHORMONER.
SMARTiSM00430. HOLI. 1 hit.
[Graphical view]
SUPFAMiSSF48508. SSF48508. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiNR0B1_MOUSE
AccessioniPrimary (citable) accession number: Q61066
Secondary accession number(s): O09068, O09147
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 1, 1996
Last modified: November 2, 2016
This is version 146 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.