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Q61066

- NR0B1_MOUSE

UniProt

Q61066 - NR0B1_MOUSE

Protein

Nuclear receptor subfamily 0 group B member 1

Gene

Nr0b1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 128 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Orphan nuclear receptor. Component of a cascade required for the development of the hypothalamic-pituitary-adrenal-gonadal axis. Acts as a coregulatory protein that inhibits the transcriptional activity of other nuclear receptors through heterodimeric interactions. May also have a role in the development of the embryo and in the maintenance of embryonic stem cell pluripotency By similarity.By similarity

    GO - Molecular functioni

    1. DNA hairpin binding Source: Ensembl
    2. ligand-activated sequence-specific DNA binding RNA polymerase II transcription factor activity Source: Ensembl
    3. protein binding Source: IntAct
    4. RNA binding Source: HGNC
    5. sequence-specific DNA binding Source: Ensembl
    6. steroid hormone receptor activity Source: InterPro
    7. transcription corepressor activity Source: MGI

    GO - Biological processi

    1. adrenal gland development Source: MGI
    2. cell differentiation Source: MGI
    3. Leydig cell differentiation Source: MGI
    4. male gonad development Source: MGI
    5. male sex determination Source: MGI
    6. negative regulation of cell differentiation Source: MGI
    7. negative regulation of intracellular steroid hormone receptor signaling pathway Source: Ensembl
    8. negative regulation of sequence-specific DNA binding transcription factor activity Source: Ensembl
    9. negative regulation of transcription, DNA-templated Source: MGI
    10. negative regulation of transcription from RNA polymerase II promoter Source: MGI
    11. protein localization Source: Ensembl
    12. response to immobilization stress Source: MGI
    13. Sertoli cell differentiation Source: MGI
    14. sex determination Source: MGI
    15. spermatogenesis Source: MGI
    16. steroid biosynthetic process Source: Ensembl

    Keywords - Molecular functioni

    Receptor, Repressor

    Keywords - Biological processi

    Transcription, Transcription regulation

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Nuclear receptor subfamily 0 group B member 1
    Alternative name(s):
    Nuclear receptor DAX-1
    Gene namesi
    Name:Nr0b1
    Synonyms:Ahch, Dax1
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome X

    Organism-specific databases

    MGIiMGI:1352460. Nr0b1.

    Subcellular locationi

    Nucleus By similarity. Cytoplasm By similarity
    Note: Shuttles between the cytoplasm and nucleus. Homodimers exits in the cytoplasm and in the nucleus By similarity.By similarity

    GO - Cellular componenti

    1. cytoplasm Source: MGI
    2. membrane Source: Ensembl
    3. nucleus Source: HGNC
    4. polysomal ribosome Source: HGNC

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 472472Nuclear receptor subfamily 0 group B member 1PRO_0000053749Add
    BLAST

    Proteomic databases

    PRIDEiQ61066.

    PTM databases

    PhosphoSiteiQ61066.

    Expressioni

    Tissue specificityi

    Expressed in adult cerebral cortex, spinal cord, thymus, heart, lung, ovary, testis, adrenal gland, hypothalamus, spleen and kidney.1 Publication

    Developmental stagei

    Expressed in E5.5 throughout the embryo except in the proximal visceral endoderm.1 Publication

    Gene expression databases

    BgeeiQ61066.
    CleanExiMM_NR0B1.
    GenevestigatoriQ61066.

    Interactioni

    Subunit structurei

    Homodimer. Interacts with NR5A1, NR5A2, NR0B2 and with COPS2 By similarity.By similarity

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    EsrrbQ615392EBI-2312665,EBI-2312731
    NanogQ80Z647EBI-2312665,EBI-2312517
    Pou5f1P202635EBI-2312665,EBI-1606219
    Rif1Q6PR542EBI-2312665,EBI-2312621
    Slc8a1P704142EBI-2312665,EBI-2312694

    Protein-protein interaction databases

    BioGridi198035. 17 interactions.
    DIPiDIP-29929N.
    IntActiQ61066. 13 interactions.

    Structurei

    Secondary structure

    1
    472
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi253 – 27220
    Helixi275 – 2784
    Helixi282 – 30322
    Helixi359 – 37214
    Helixi377 – 38812
    Helixi399 – 42022
    Helixi426 – 43813
    Helixi445 – 4506
    Helixi452 – 4554
    Helixi460 – 4678

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3F5CX-ray3.00B/C205-472[»]
    ProteinModelPortaliQ61066.
    SMRiQ61066. Positions 251-472.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ61066.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati1 – 67671Add
    BLAST
    Repeati68 – 135682Add
    BLAST
    Repeati136 – 202673Add
    BLAST
    Repeati203 – 255534; truncatedAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 2552554 X 67 AA tandem repeatsAdd
    BLAST
    Regioni256 – 472217Ligand-bindingBy similarityAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi13 – 175LXXLL motif 1
    Motifi80 – 845LXXLL motif 2
    Motifi148 – 1525LXXLL motif 3
    Motifi463 – 4686AF-2 motif

    Domaini

    Homodimerization involved an interaction between amino and carboxy termini involving LXXLL motifs and steroid binding domain (AF-2 motif). Heterodimerizes with NR5A1 and NROB2 through its N-terminal LXXLL motifs By similarity.By similarity

    Sequence similaritiesi

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiNOG238310.
    GeneTreeiENSGT00390000015719.
    HOGENOMiHOG000231151.
    HOVERGENiHBG005453.
    InParanoidiQ61066.
    KOiK08562.
    OMAiCCFCGED.
    OrthoDBiEOG7NSB35.
    PhylomeDBiQ61066.
    TreeFamiTF332386.

    Family and domain databases

    Gene3Di1.10.565.10. 1 hit.
    InterProiIPR008946. Nucl_hormone_rcpt_ligand-bd.
    IPR000536. Nucl_hrmn_rcpt_lig-bd_core.
    IPR025900. Nuclear_receptor_repeat.
    IPR001723. Str_hrmn_rcpt.
    [Graphical view]
    PfamiPF00104. Hormone_recep. 1 hit.
    PF14046. NR_Repeat. 4 hits.
    [Graphical view]
    PRINTSiPR00398. STRDHORMONER.
    SMARTiSM00430. HOLI. 1 hit.
    [Graphical view]
    SUPFAMiSSF48508. SSF48508. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q61066-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAGEDHPWQG SILYNLLMSA KQKHASQEER EVRLGAQCWG CACGAQPVLG    50
    GERLSGGQAR SLLYRCCFCG ENHPRQGGIL YSMLTNARQP SVATQAPRAR 100
    FGAPCWGCAC GSAEPLVGRE GLPAGQAPSL LYRCCFCGEE HPRQGSILYS 150
    LLTSAQQTHV SREAPEAHRR GEWWQLSYCT QSVGGPEGLQ STQAMAFLYR 200
    SYVCGEEQPQ QISVASGTPV SADQTPATPQ EQPRAPWWDA SPGVQRLITL 250
    KDPQVVCEAA SAGLLKTLRF VKYLPCFQIL PLDQQLVLVR SCWAPLLMLE 300
    LAQDHLHFEM MEIPETNTTQ EMLTTRRQET EGPEPAEPQA TEQPQMVSAE 350
    AGHLLPAAAV QAIKSFFFKC WSLNIDTKEY AYLKGTVLFN PDLPGLQCVK 400
    YIEGLQWRTQ QILTEHIRMM QREYQIRSAE LNSALFLLRF INSDVVTELF 450
    FRPIIGAVSM DDMMLEMLCA KL 472
    Length:472
    Mass (Da):52,576
    Last modified:November 1, 1996 - v1
    Checksum:iDE1FB01C30F883CF
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    S83180, S83178 Genomic DNA. Translation: AAB46875.1.
    U41568 mRNA. Translation: AAC52920.1.
    CCDSiCCDS30260.1.
    PIRiJC5199.
    RefSeqiNP_031456.1. NM_007430.4.
    UniGeneiMm.5180.

    Genome annotation databases

    EnsembliENSMUST00000026036; ENSMUSP00000026036; ENSMUSG00000025056.
    GeneIDi11614.
    KEGGimmu:11614.
    UCSCiuc009tsd.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    S83180 , S83178 Genomic DNA. Translation: AAB46875.1 .
    U41568 mRNA. Translation: AAC52920.1 .
    CCDSi CCDS30260.1.
    PIRi JC5199.
    RefSeqi NP_031456.1. NM_007430.4.
    UniGenei Mm.5180.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3F5C X-ray 3.00 B/C 205-472 [» ]
    ProteinModelPortali Q61066.
    SMRi Q61066. Positions 251-472.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 198035. 17 interactions.
    DIPi DIP-29929N.
    IntActi Q61066. 13 interactions.

    PTM databases

    PhosphoSitei Q61066.

    Proteomic databases

    PRIDEi Q61066.

    Protocols and materials databases

    DNASUi 11614.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000026036 ; ENSMUSP00000026036 ; ENSMUSG00000025056 .
    GeneIDi 11614.
    KEGGi mmu:11614.
    UCSCi uc009tsd.1. mouse.

    Organism-specific databases

    CTDi 190.
    MGIi MGI:1352460. Nr0b1.

    Phylogenomic databases

    eggNOGi NOG238310.
    GeneTreei ENSGT00390000015719.
    HOGENOMi HOG000231151.
    HOVERGENi HBG005453.
    InParanoidi Q61066.
    KOi K08562.
    OMAi CCFCGED.
    OrthoDBi EOG7NSB35.
    PhylomeDBi Q61066.
    TreeFami TF332386.

    Miscellaneous databases

    EvolutionaryTracei Q61066.
    NextBioi 279166.
    PROi Q61066.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q61066.
    CleanExi MM_NR0B1.
    Genevestigatori Q61066.

    Family and domain databases

    Gene3Di 1.10.565.10. 1 hit.
    InterProi IPR008946. Nucl_hormone_rcpt_ligand-bd.
    IPR000536. Nucl_hrmn_rcpt_lig-bd_core.
    IPR025900. Nuclear_receptor_repeat.
    IPR001723. Str_hrmn_rcpt.
    [Graphical view ]
    Pfami PF00104. Hormone_recep. 1 hit.
    PF14046. NR_Repeat. 4 hits.
    [Graphical view ]
    PRINTSi PR00398. STRDHORMONER.
    SMARTi SM00430. HOLI. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48508. SSF48508. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Ahch, the mouse homologue of DAX1: cloning, characterization and synteny with GyK, the glycerol kinase locus."
      Guo W., Lovell R.S., Zhang Y.H., Huang B.L., Burris T.P., Craigen W.J., McCabe E.R.B.
      Gene 178:31-34(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: CD-1.
      Tissue: Testis.
    2. "Characterization of the mouse DAX-1 gene reveals evolutionary conservation of a unique amino-terminal motif and widespread expression in mouse tissue."
      Bae D.S., Schaefer M.L., Partan B.W., Muglia L.
      Endocrinology 137:3921-3927(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], TISSUE SPECIFICITY.
    3. "Novel role for the orphan nuclear receptor Dax1 in embryogenesis, different from steroidogenesis."
      Niakan K.K., Davis E.C., Clipsham R.C., Jiang M., Dehart D.B., Sulik K.K., McCabe E.R.B.
      Mol. Genet. Metab. 88:261-271(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: DEVELOPMENTAL STAGE, FUNCTION.

    Entry informationi

    Entry nameiNR0B1_MOUSE
    AccessioniPrimary (citable) accession number: Q61066
    Secondary accession number(s): O09068, O09147
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 30, 2000
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 128 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3