Nuclear receptor subfamily 0 group B member 1

Contribute

Send feedback

Read comments (?) or add your own

Q61066 (NR0B1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 117. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

text xml rdf/xml gff fasta

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Nuclear receptor subfamily 0 group B member 1

Alternative name(s):
Nuclear receptor DAX-1

Gene names

Name:	Nr0b1
Synonyms:	Ahch, Dax1

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	472 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Orphan nuclear receptor. Component of a cascade required for the development of the hypothalamic-pituitary-adrenal-gonadal axis. Acts as a coregulatory protein that inhibits the transcriptional activity of other nuclear receptors through heterodimeric interactions. May also have a role in the development of the embryo and in the maintenance of embryonic stem cell pluripotency By similarity. Ref.3
Subunit structure	Homodimer. Interacts with NR5A1, NR5A2, NR0B2 and with COPS2 By similarity.
Subcellular location	Nucleus By similarity. Cytoplasm By similarity. Note: Shuttles between the cytoplasm and nucleus. Homodimers exits in the cytoplasm and in the nucleus By similarity.
Tissue specificity	Expressed in adult cerebral cortex, spinal cord, thymus, heart, lung, ovary, testis, adrenal gland, hypothalamus, spleen and kidney. Ref.2
Developmental stage	Expressed in E5.5 throughout the embryo except in the proximal visceral endoderm. Ref.3
Domain	Homodimerization involved an interaction between amino and carboxy termini involving LXXLL motifs and steroid binding domain (AF-2 motif). Heterodimerizes with NR5A1 and NROB2 through its N-terminal LXXLL motifs By similarity.
Sequence similarities	Belongs to the nuclear hormone receptor family. NR0 subfamily.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Cytoplasm Nucleus
Domain	Repeat
Molecular function	Receptor Repressor
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	Leydig cell differentiation Inferred from mutant phenotype PubMed 11564714. Source: MGI Sertoli cell differentiation Inferred from mutant phenotype PubMed 11564714. Source: MGI adrenal gland development Inferred from genetic interaction PubMed 11796523. Source: MGI male sex determination Inferred from mutant phenotype PubMed 12679814 PubMed 15944188. Source: MGI negative regulation of cell differentiation Inferred from mutant phenotype Ref.3. Source: MGI negative regulation of intracellular steroid hormone receptor signaling pathway Inferred from electronic annotation. Source: Compara negative regulation of sequence-specific DNA binding transcription factor activity Inferred from electronic annotation. Source: Compara negative regulation of transcription from RNA polymerase II promoter Inferred from mutant phenotype PubMed 15155786. Source: MGI protein localization Inferred from electronic annotation. Source: Compara response to stress Inferred from mutant phenotype PubMed 11796523. Source: MGI spermatogenesis Inferred from mutant phenotype PubMed 11356697 PubMed 9843206. Source: MGI steroid biosynthetic process Inferred from electronic annotation. Source: Compara
Cellular_component	membrane Inferred from electronic annotation. Source: Compara nucleus Inferred from direct assay PubMed 10848616. Source: HGNC polysomal ribosome Inferred from direct assay PubMed 10848616. Source: HGNC
Molecular_function	DNA hairpin binding Inferred from electronic annotation. Source: Compara RNA binding Inferred from direct assay PubMed 10848616. Source: HGNC ligand-activated sequence-specific DNA binding RNA polymerase II transcription factor activity Inferred from electronic annotation. Source: Compara sequence-specific DNA binding Inferred from electronic annotation. Source: Compara steroid hormone receptor activity Inferred from electronic annotation. Source: InterPro transcription corepressor activity Inferred from genetic interaction PubMed 15155786. Source: MGI
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct
Esrrb	Q61539	2	EBI-2312665,EBI-2312731
Nanog	Q80Z64	6	EBI-2312665,EBI-2312517
Pou5f1	P20263	5	EBI-2312665,EBI-1606219
Rif1	Q6PR54	2	EBI-2312665,EBI-2312621
Slc8a1	P70414	2	EBI-2312665,EBI-2312694

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 472

472

Nuclear receptor subfamily 0 group B member 1

PRO_0000053749

Regions

Repeat

Repeat

Repeat

Repeat

4; truncated

Region

1 – 255

255

4 X 67 AA tandem repeats

Region

256 – 472

217

Ligand-binding By similarity

Motif

13 – 17

LXXLL motif 1

Motif

80 – 84

LXXLL motif 2

Motif

148 – 152

LXXLL motif 3

Motif

463 – 468

AF-2 motif

Secondary structure

472

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

Q61066 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: DE1FB01C30F883CF
Show »

FASTA

472

52,576

        10         20         30         40         50         60 
MAGEDHPWQG SILYNLLMSA KQKHASQEER EVRLGAQCWG CACGAQPVLG GERLSGGQAR 

        70         80         90        100        110        120 
SLLYRCCFCG ENHPRQGGIL YSMLTNARQP SVATQAPRAR FGAPCWGCAC GSAEPLVGRE 

       130        140        150        160        170        180 
GLPAGQAPSL LYRCCFCGEE HPRQGSILYS LLTSAQQTHV SREAPEAHRR GEWWQLSYCT 

       190        200        210        220        230        240 
QSVGGPEGLQ STQAMAFLYR SYVCGEEQPQ QISVASGTPV SADQTPATPQ EQPRAPWWDA 

       250        260        270        280        290        300 
SPGVQRLITL KDPQVVCEAA SAGLLKTLRF VKYLPCFQIL PLDQQLVLVR SCWAPLLMLE 

       310        320        330        340        350        360 
LAQDHLHFEM MEIPETNTTQ EMLTTRRQET EGPEPAEPQA TEQPQMVSAE AGHLLPAAAV 

       370        380        390        400        410        420 
QAIKSFFFKC WSLNIDTKEY AYLKGTVLFN PDLPGLQCVK YIEGLQWRTQ QILTEHIRMM 

       430        440        450        460        470 
QREYQIRSAE LNSALFLLRF INSDVVTELF FRPIIGAVSM DDMMLEMLCA KL

« Hide

References

[1]	"Ahch, the mouse homologue of DAX1: cloning, characterization and synteny with GyK, the glycerol kinase locus." Guo W., Lovell R.S., Zhang Y.H., Huang B.L., Burris T.P., Craigen W.J., McCabe E.R.B. Gene 178:31-34(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: CD-1. Tissue: Testis.
[2]	"Characterization of the mouse DAX-1 gene reveals evolutionary conservation of a unique amino-terminal motif and widespread expression in mouse tissue." Bae D.S., Schaefer M.L., Partan B.W., Muglia L. Endocrinology 137:3921-3927(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], TISSUE SPECIFICITY.
[3]	"Novel role for the orphan nuclear receptor Dax1 in embryogenesis, different from steroidogenesis." Niakan K.K., Davis E.C., Clipsham R.C., Jiang M., Dehart D.B., Sulik K.K., McCabe E.R.B. Mol. Genet. Metab. 88:261-271(2006) [PubMed] [Europe PMC] [Abstract] Cited for: DEVELOPMENTAL STAGE, FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

S83180, S83178 Genomic DNA. Translation: AAB46875.1.
U41568 mRNA. Translation: AAC52920.1.

IPI

IPI00117060.

PIR

JC5199.

RefSeq

NP_031456.1. NM_007430.4.

UniGene

Mm.5180.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3F5C	X-ray	3.00	B/C	205-472	[»]

ProteinModelPortal

Q61066.

SMR

Q61066. Positions 251-472.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-29929N.

IntAct

Q61066. 13 interactions.

PTM databases

PhosphoSite

Q61066.

Proteomic databases

PRIDE

Q61066.

Protocols and materials databases

DNASU

11614.

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENSMUST00000026036; ENSMUSP00000026036; ENSMUSG00000025056.

GeneID

11614.

KEGG

mmu:11614.

Organism-specific databases

CTD

190.

MGI

MGI:1352460. Nr0b1.

Phylogenomic databases

eggNOG

NOG238310.

GeneTree

ENSGT00390000015719.

HOGENOM

HOG000231151.

HOVERGEN

HBG005453.

InParanoid

Q61066.

K08562.

OrthoDB

EOG48D0V9.

Gene expression databases

ArrayExpress

Q61066.

Bgee

Q61066.

CleanEx

MM_NR0B1.

Genevestigator

Q61066.

GermOnline

ENSMUSG00000025056. Mus musculus.

Family and domain databases

Gene3D

1.10.565.10. 1 hit.

InterPro

IPR008946. Nucl_hormone_rcpt_ligand-bd.
IPR000536. Nucl_hrmn_rcpt_lig-bd_core.
IPR025900. Nuclear_receptor_repeat.
IPR001723. Str_hrmn_rcpt.
[Graphical view]

Pfam

PF00104. Hormone_recep. 1 hit.
PF14046. NR_Repeat. 4 hits.
[Graphical view]

PRINTS

PR00398. STRDHORMONER.

SMART

SM00430. HOLI. 1 hit.
[Graphical view]

SUPFAM

SSF48508. Str_ncl_receptor. 1 hit.

ProtoNet

Search...

Other

EvolutionaryTrace

Q61066.

NextBio

279166.

SOURCE

Search...

Entry information

Entry name

NR0B1_MOUSE

Accession

Primary (citable) accession number: Q61066
Secondary accession number(s): O09068, O09147

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	November 1, 1996
Last modified:	May 1, 2013
This is version 117 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents