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Q61066 (NR0B1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 127. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Nuclear receptor subfamily 0 group B member 1
Alternative name(s):
Nuclear receptor DAX-1
Gene names
Name:Nr0b1
Synonyms:Ahch, Dax1
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length472 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Orphan nuclear receptor. Component of a cascade required for the development of the hypothalamic-pituitary-adrenal-gonadal axis. Acts as a coregulatory protein that inhibits the transcriptional activity of other nuclear receptors through heterodimeric interactions. May also have a role in the development of the embryo and in the maintenance of embryonic stem cell pluripotency By similarity. Ref.3

Subunit structure

Homodimer. Interacts with NR5A1, NR5A2, NR0B2 and with COPS2 By similarity.

Subcellular location

Nucleus By similarity. Cytoplasm By similarity. Note: Shuttles between the cytoplasm and nucleus. Homodimers exits in the cytoplasm and in the nucleus By similarity.

Tissue specificity

Expressed in adult cerebral cortex, spinal cord, thymus, heart, lung, ovary, testis, adrenal gland, hypothalamus, spleen and kidney. Ref.2

Developmental stage

Expressed in E5.5 throughout the embryo except in the proximal visceral endoderm. Ref.3

Domain

Homodimerization involved an interaction between amino and carboxy termini involving LXXLL motifs and steroid binding domain (AF-2 motif). Heterodimerizes with NR5A1 and NROB2 through its N-terminal LXXLL motifs By similarity.

Sequence similarities

Belongs to the nuclear hormone receptor family. NR0 subfamily.

Ontologies

Keywords
   Biological processTranscription
Transcription regulation
   Cellular componentCytoplasm
Nucleus
   DomainRepeat
   Molecular functionReceptor
Repressor
   Technical term3D-structure
Complete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processLeydig cell differentiation

Inferred from mutant phenotype PubMed 11564714. Source: MGI

Sertoli cell differentiation

Inferred from mutant phenotype PubMed 11564714. Source: MGI

adrenal gland development

Inferred from genetic interaction PubMed 11796523. Source: MGI

cell differentiation

Inferred from genetic interaction PubMed 15829514. Source: MGI

male gonad development

Inferred from mutant phenotype PubMed 12679814. Source: MGI

male sex determination

Inferred from mutant phenotype PubMed 12679814PubMed 15944188. Source: MGI

negative regulation of cell differentiation

Inferred from mutant phenotype Ref.3. Source: MGI

negative regulation of intracellular steroid hormone receptor signaling pathway

Inferred from electronic annotation. Source: Ensembl

negative regulation of sequence-specific DNA binding transcription factor activity

Inferred from electronic annotation. Source: Ensembl

negative regulation of transcription from RNA polymerase II promoter

Inferred from mutant phenotype PubMed 15155786. Source: MGI

negative regulation of transcription, DNA-templated

Inferred from direct assay PubMed 15062575. Source: MGI

protein localization

Inferred from electronic annotation. Source: Ensembl

response to immobilization stress

Inferred from mutant phenotype PubMed 11796523. Source: MGI

sex determination

Inferred from genetic interaction PubMed 9486644. Source: MGI

spermatogenesis

Inferred from mutant phenotype PubMed 11356697PubMed 9843206. Source: MGI

steroid biosynthetic process

Inferred from electronic annotation. Source: Ensembl

   Cellular_componentcytoplasm

Inferred from direct assay PubMed 12610109PubMed 14678822Ref.3. Source: MGI

membrane

Inferred from electronic annotation. Source: Ensembl

nucleus

Inferred from direct assay PubMed 10848616. Source: HGNC

polysomal ribosome

Inferred from direct assay PubMed 10848616. Source: HGNC

   Molecular_functionDNA hairpin binding

Inferred from electronic annotation. Source: Ensembl

RNA binding

Inferred from direct assay PubMed 10848616. Source: HGNC

ligand-activated sequence-specific DNA binding RNA polymerase II transcription factor activity

Inferred from electronic annotation. Source: Ensembl

protein binding

Inferred from physical interaction PubMed 17093407PubMed 20362541PubMed 23892456. Source: IntAct

sequence-specific DNA binding

Inferred from electronic annotation. Source: Ensembl

steroid hormone receptor activity

Inferred from electronic annotation. Source: InterPro

transcription corepressor activity

Inferred from genetic interaction PubMed 15155786. Source: MGI

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 472472Nuclear receptor subfamily 0 group B member 1
PRO_0000053749

Regions

Repeat1 – 67671
Repeat68 – 135682
Repeat136 – 202673
Repeat203 – 255534; truncated
Region1 – 2552554 X 67 AA tandem repeats
Region256 – 472217Ligand-binding By similarity
Motif13 – 175LXXLL motif 1
Motif80 – 845LXXLL motif 2
Motif148 – 1525LXXLL motif 3
Motif463 – 4686AF-2 motif

Secondary structure

..................... 472
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q61066 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: DE1FB01C30F883CF

FASTA47252,576
        10         20         30         40         50         60 
MAGEDHPWQG SILYNLLMSA KQKHASQEER EVRLGAQCWG CACGAQPVLG GERLSGGQAR 

        70         80         90        100        110        120 
SLLYRCCFCG ENHPRQGGIL YSMLTNARQP SVATQAPRAR FGAPCWGCAC GSAEPLVGRE 

       130        140        150        160        170        180 
GLPAGQAPSL LYRCCFCGEE HPRQGSILYS LLTSAQQTHV SREAPEAHRR GEWWQLSYCT 

       190        200        210        220        230        240 
QSVGGPEGLQ STQAMAFLYR SYVCGEEQPQ QISVASGTPV SADQTPATPQ EQPRAPWWDA 

       250        260        270        280        290        300 
SPGVQRLITL KDPQVVCEAA SAGLLKTLRF VKYLPCFQIL PLDQQLVLVR SCWAPLLMLE 

       310        320        330        340        350        360 
LAQDHLHFEM MEIPETNTTQ EMLTTRRQET EGPEPAEPQA TEQPQMVSAE AGHLLPAAAV 

       370        380        390        400        410        420 
QAIKSFFFKC WSLNIDTKEY AYLKGTVLFN PDLPGLQCVK YIEGLQWRTQ QILTEHIRMM 

       430        440        450        460        470 
QREYQIRSAE LNSALFLLRF INSDVVTELF FRPIIGAVSM DDMMLEMLCA KL 

« Hide

References

[1]"Ahch, the mouse homologue of DAX1: cloning, characterization and synteny with GyK, the glycerol kinase locus."
Guo W., Lovell R.S., Zhang Y.H., Huang B.L., Burris T.P., Craigen W.J., McCabe E.R.B.
Gene 178:31-34(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: CD-1.
Tissue: Testis.
[2]"Characterization of the mouse DAX-1 gene reveals evolutionary conservation of a unique amino-terminal motif and widespread expression in mouse tissue."
Bae D.S., Schaefer M.L., Partan B.W., Muglia L.
Endocrinology 137:3921-3927(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], TISSUE SPECIFICITY.
[3]"Novel role for the orphan nuclear receptor Dax1 in embryogenesis, different from steroidogenesis."
Niakan K.K., Davis E.C., Clipsham R.C., Jiang M., Dehart D.B., Sulik K.K., McCabe E.R.B.
Mol. Genet. Metab. 88:261-271(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: DEVELOPMENTAL STAGE, FUNCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
S83180, S83178 Genomic DNA. Translation: AAB46875.1.
U41568 mRNA. Translation: AAC52920.1.
CCDSCCDS30260.1.
PIRJC5199.
RefSeqNP_031456.1. NM_007430.4.
UniGeneMm.5180.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
3F5CX-ray3.00B/C205-472[»]
ProteinModelPortalQ61066.
SMRQ61066. Positions 251-472.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid198035. 17 interactions.
DIPDIP-29929N.
IntActQ61066. 13 interactions.

PTM databases

PhosphoSiteQ61066.

Proteomic databases

PRIDEQ61066.

Protocols and materials databases

DNASU11614.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000026036; ENSMUSP00000026036; ENSMUSG00000025056.
GeneID11614.
KEGGmmu:11614.
UCSCuc009tsd.1. mouse.

Organism-specific databases

CTD190.
MGIMGI:1352460. Nr0b1.

Phylogenomic databases

eggNOGNOG238310.
GeneTreeENSGT00390000015719.
HOGENOMHOG000231151.
HOVERGENHBG005453.
InParanoidQ61066.
KOK08562.
OMACCFCGED.
OrthoDBEOG7NSB35.
PhylomeDBQ61066.
TreeFamTF332386.

Gene expression databases

BgeeQ61066.
CleanExMM_NR0B1.
GenevestigatorQ61066.

Family and domain databases

Gene3D1.10.565.10. 1 hit.
InterProIPR008946. Nucl_hormone_rcpt_ligand-bd.
IPR000536. Nucl_hrmn_rcpt_lig-bd_core.
IPR025900. Nuclear_receptor_repeat.
IPR001723. Str_hrmn_rcpt.
[Graphical view]
PfamPF00104. Hormone_recep. 1 hit.
PF14046. NR_Repeat. 4 hits.
[Graphical view]
PRINTSPR00398. STRDHORMONER.
SMARTSM00430. HOLI. 1 hit.
[Graphical view]
SUPFAMSSF48508. SSF48508. 1 hit.
ProtoNetSearch...

Other

EvolutionaryTraceQ61066.
NextBio279166.
PROQ61066.
SOURCESearch...

Entry information

Entry nameNR0B1_MOUSE
AccessionPrimary (citable) accession number: Q61066
Secondary accession number(s): O09068, O09147
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 1, 1996
Last modified: July 9, 2014
This is version 127 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot