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Protein

WW domain-binding protein 4

Gene

Wbp4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Promotes pre-mRNA splicing. A spliceosome-associated protein; may play a role in cross-intron bridging of U1 and U2 snRNPs in the mammalian A complex.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri11 – 42Matrin-typePROSITE-ProRule annotationAdd BLAST32

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

mRNA processing, mRNA splicing

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-MMU-72163. mRNA Splicing - Major Pathway.

Names & Taxonomyi

Protein namesi
Recommended name:
WW domain-binding protein 4
Short name:
WBP-4
Alternative name(s):
Formin-binding protein 21
WW domain-containing-binding protein 4
Gene namesi
Name:Wbp4
Synonyms:Fbp21, Fnbp21
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 14

Organism-specific databases

MGIiMGI:109568. Wbp4.

Subcellular locationi

  • Nucleus speckle PROSITE-ProRule annotation1 Publication

GO - Cellular componenti

  • nuclear speck Source: UniProtKB
  • nucleoplasm Source: MGI
  • plasma membrane Source: MGI
  • spliceosomal complex Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Nucleus, Spliceosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000760661 – 376WW domain-binding protein 4Add BLAST376

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei228PhosphoserineBy similarity1
Modified residuei230PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ61048.
MaxQBiQ61048.
PaxDbiQ61048.
PRIDEiQ61048.

PTM databases

iPTMnetiQ61048.
PhosphoSitePlusiQ61048.

Expressioni

Gene expression databases

BgeeiENSMUSG00000022023.
CleanExiMM_WBP4.
GenevisibleiQ61048. MM.

Interactioni

Subunit structurei

Associated with U2 snRNPs. Interacts via the WW domains with the Pro-rich domains of WBP11 (By similarity). Binds splicing factors SNRPB, SNRPC and SF1. Interacts via the WW domains with the Pro-rich domains of KHDRBS1/SAM68.By similarity2 Publications

GO - Molecular functioni

Protein-protein interaction databases

BioGridi204548. 1 interactor.
STRINGi10090.ENSMUSP00000022601.

Structurei

3D structure databases

ProteinModelPortaliQ61048.
SMRiQ61048.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini123 – 156WW 1PROSITE-ProRule annotationAdd BLAST34
Domaini164 – 197WW 2PROSITE-ProRule annotationAdd BLAST34

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi113 – 122Poly-Lys10
Compositional biasi115 – 126Lys-richAdd BLAST12

Domaini

The WW domain recognizes the proline, glycine and methionine-rich (PGM) motif present in the splicing factors, as well as the Arg/Gly-rich-flanked Pro-rich domains found in several WW domain-binding proteins.

Sequence similaritiesi

Contains 1 matrin-type zinc finger.PROSITE-ProRule annotation
Contains 2 WW domains.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri11 – 42Matrin-typePROSITE-ProRule annotationAdd BLAST32

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG0150. Eukaryota.
ENOG4111SKN. LUCA.
GeneTreeiENSGT00390000013956.
HOGENOMiHOG000067962.
HOVERGENiHBG053152.
InParanoidiQ61048.
KOiK13220.
OMAiNPYGAWE.
OrthoDBiEOG091G0GZ4.
TreeFamiTF316671.

Family and domain databases

InterProiIPR001202. WW_dom.
IPR000690. Znf_C2H2_matrin.
IPR003604. Znf_U1.
IPR013085. Znf_U1-C.
[Graphical view]
PfamiPF00397. WW. 2 hits.
PF06220. zf-U1. 1 hit.
[Graphical view]
SMARTiSM00456. WW. 2 hits.
SM00451. ZnF_U1. 1 hit.
[Graphical view]
SUPFAMiSSF51045. SSF51045. 2 hits.
PROSITEiPS01159. WW_DOMAIN_1. 1 hit.
PS50020. WW_DOMAIN_2. 2 hits.
PS50171. ZF_MATRIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q61048-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MADYWKSQPK KFCDYCKCWI ADNRPSVEFH ERGKNHKENV ARRISEIKQK
60 70 80 90 100
SLDKAKEEEK ASKEFAAMEA AALKAYQEDL KRLGLPLPSD ISEPTVSPVI
110 120 130 140 150
STVQPTPTSN QQKEKKKKKK KKEASKGGWV EGVTADGHCY YYDLITGASQ
160 170 180 190 200
WEKPEGFQGN LKKTAAKAVW VEGLSEDGYT YYYNTETGES KWEKPEDFIP
210 220 230 240 250
HGGDVLSSKD SGKLPDTLED AKSSDSHSDS EGEQKKAGEA STETKKLIIK
260 270 280 290 300
FKEKNKSTEK RIGPEIQKEK STPKQNPSNT NEEKPKTLKK STNPYGEWQE
310 320 330 340 350
IKQEAESQEE VDLELPSTEG ECLSTSEAGV GEIKVVFKEK TVSSLGVAAD
360 370
GVAPVFKKRR LENGKSRNLR QRGDDE
Length:376
Mass (Da):42,137
Last modified:July 27, 2011 - v4
Checksum:iECA7288FC94F3F83
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti80 – 83LKRL → SEKA in AAC34810 (PubMed:9724750).Curated4
Sequence conflicti155E → K in AAH34851 (Ref. 3) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF071184 mRNA. Translation: AAC34810.1.
AK019160 mRNA. Translation: BAB31575.3.
AK135215 mRNA. Translation: BAE22462.1.
AK160559 mRNA. Translation: BAE35873.1.
AK162009 mRNA. Translation: BAE36679.1.
CH466535 Genomic DNA. Translation: EDL35764.1.
BC034851 mRNA. Translation: AAH34851.1.
CCDSiCCDS27300.1.
PIRiS64714.
RefSeqiNP_061235.2. NM_018765.3.
UniGeneiMm.476813.

Genome annotation databases

EnsembliENSMUST00000022601; ENSMUSP00000022601; ENSMUSG00000022023.
GeneIDi22380.
KEGGimmu:22380.
UCSCiuc007uta.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF071184 mRNA. Translation: AAC34810.1.
AK019160 mRNA. Translation: BAB31575.3.
AK135215 mRNA. Translation: BAE22462.1.
AK160559 mRNA. Translation: BAE35873.1.
AK162009 mRNA. Translation: BAE36679.1.
CH466535 Genomic DNA. Translation: EDL35764.1.
BC034851 mRNA. Translation: AAH34851.1.
CCDSiCCDS27300.1.
PIRiS64714.
RefSeqiNP_061235.2. NM_018765.3.
UniGeneiMm.476813.

3D structure databases

ProteinModelPortaliQ61048.
SMRiQ61048.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi204548. 1 interactor.
STRINGi10090.ENSMUSP00000022601.

PTM databases

iPTMnetiQ61048.
PhosphoSitePlusiQ61048.

Proteomic databases

EPDiQ61048.
MaxQBiQ61048.
PaxDbiQ61048.
PRIDEiQ61048.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000022601; ENSMUSP00000022601; ENSMUSG00000022023.
GeneIDi22380.
KEGGimmu:22380.
UCSCiuc007uta.1. mouse.

Organism-specific databases

CTDi11193.
MGIiMGI:109568. Wbp4.

Phylogenomic databases

eggNOGiKOG0150. Eukaryota.
ENOG4111SKN. LUCA.
GeneTreeiENSGT00390000013956.
HOGENOMiHOG000067962.
HOVERGENiHBG053152.
InParanoidiQ61048.
KOiK13220.
OMAiNPYGAWE.
OrthoDBiEOG091G0GZ4.
TreeFamiTF316671.

Enzyme and pathway databases

ReactomeiR-MMU-72163. mRNA Splicing - Major Pathway.

Miscellaneous databases

ChiTaRSiWbp4. mouse.
PROiQ61048.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000022023.
CleanExiMM_WBP4.
GenevisibleiQ61048. MM.

Family and domain databases

InterProiIPR001202. WW_dom.
IPR000690. Znf_C2H2_matrin.
IPR003604. Znf_U1.
IPR013085. Znf_U1-C.
[Graphical view]
PfamiPF00397. WW. 2 hits.
PF06220. zf-U1. 1 hit.
[Graphical view]
SMARTiSM00456. WW. 2 hits.
SM00451. ZnF_U1. 1 hit.
[Graphical view]
SUPFAMiSSF51045. SSF51045. 2 hits.
PROSITEiPS01159. WW_DOMAIN_1. 1 hit.
PS50020. WW_DOMAIN_2. 2 hits.
PS50171. ZF_MATRIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiWBP4_MOUSE
AccessioniPrimary (citable) accession number: Q61048
Secondary accession number(s): Q3TUU8, Q8K1Z9, Q9CS45
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 7, 2005
Last sequence update: July 27, 2011
Last modified: November 2, 2016
This is version 118 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.